Heterozygous Essentials B isolates Release 1 Synced to SGD on Fri Aug 1 09:00:53 2008 plate row column ORF Gene Alias Description GO Aspect GO Term record no. strain RG notes DB NOTES 401 A 2 YAL043C PTA1 FUN39 Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII C, F, P RNA binding, RNA metabolic process, nucleus, transcription 20362 chr1_1 - - 401 A 3 YAL041W CDC24 CLS4 Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing C, F, P anatomical structure morphogenesis, cell budding, cell cycle, cellular bud, conjugation, enzyme regulator activity, nucleus, other, pseudohyphal growth, response to chemical stimulus, signal transduction, site of polarized growth 20365 chr1_1 met- did not mate 401 A 4 YAL038W CDC19 PYK1 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration C, F, P carbohydrate metabolic process, cytoplasm, generation of precursor metabolites and energy, other, transferase activity 20368 chr1_1 - - 401 A 5 YAL034W-a - - - - - 20372 chr1_1 - - 401 A 6 YAL035C-a - - - - - 20373 chr1_1 - - 401 A 7 YAL033W POP5 FUN53 Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends C, F, P RNA metabolic process, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 20375 chr1_1 - - 401 A 8 YAL032C PRP45 FUN20 Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 20376 chr1_1 - - 401 A 9 YAL025C MAK16 - Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus C, F, P RNA metabolic process, molecular_function, nucleolus, organelle organization and biogenesis, ribosome biogenesis 20383 chr1_1 - - 401 A 10 YAL003W EFB1 TEF5 Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site C, F, P cytoplasm, ribosome, translation, translation regulator activity 20404 chr1_1 - - 401 A 11 YAL001C TFC3 TSV115|FUN24 Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding C, F, P RNA metabolic process, mitochondrion, nucleus, transcription, transcription regulator activity 20406 chr1_1 - - 401 A 12 YAR007C RFA1 RPA1|FUN3|BUF2 Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination C, F, P DNA binding, DNA metabolic process, cell cycle, chromosome, cytoplasm, meiosis, nucleus, organelle organization and biogenesis, other, response to stress 20409 chr1_1 - - 401 B 1 YAR008W SEN34 FUN4 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease C, F, P RNA metabolic process, cytoplasm, endomembrane system, hydrolase activity, membrane, mitochondrial envelope, mitochondrion, nucleus 20410 chr1_1 - - 401 B 2 YAR019C CDC15 LYT1 Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p C, F, P cell cycle, cellular bud, cytokinesis, cytoplasm, cytoskeleton, microtubule organizing center, protein kinase activity, protein modification process, site of polarized growth 20416 chr1_1 - - 401 B 3 YLL003W SFI1 - Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C C, F, P cell cycle, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, molecular_function, organelle organization and biogenesis 21491 chr12_1 - - 401 B 4 YLL004W ORC3 - Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing C, F, P DNA binding, DNA metabolic process, RNA metabolic process, chromosome, nucleus, organelle organization and biogenesis, other, transcription 21492 chr12_1 - - 401 B 5 YLL008W DRS1 - Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles C, F, P RNA metabolic process, helicase activity, hydrolase activity, nucleolus, organelle organization and biogenesis, ribosome biogenesis 21496 chr12_1 - - 401 B 6 YLL011W SOF1 - Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 21499 chr12_1 - - 401 B 7 YLL018C DPS1 - Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation C, F, P RNA binding, cytoplasm, ligase activity, translation 21506 chr12_1 - - 401 B 8 YLL031C GPI13 MPC1 ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein C, F, P endoplasmic reticulum, lipid metabolic process, protein modification process, transferase activity 21519 chr12_1 - - 401 B 9 YLL034C RIX7 - Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions C, F, P hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transport 21522 chr12_1 - - 401 B 10 YLL035W GRC3 - Protein of unknown function, required for cell growth and possibly involved in rRNA processing; mRNA is cell cycle regulated C, F, P RNA metabolic process, cellular_component, molecular_function, organelle organization and biogenesis, ribosome biogenesis 21523 chr12_1 - - 401 B 11 YLL036C PRP19 PSO4 Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases C, F, P RNA metabolic process, cytoplasm, mitochondrion, nucleus, other, protein catabolic process 21524 chr12_1 - - 401 B 12 YLL037W - - Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor C, F, P biological_process, cellular_component, molecular_function 21525 chr12_1 - - 401 C 1 YLL050C COF1 - Cofilin, promotes actin filament depolarization in a pH-dependent manner; binds both actin monomers and filaments and severs filaments; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes C, F, P cell cortex, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, organelle organization and biogenesis, protein binding 21538 chr12_1 - - 401 C 2 YLR002C NOC3 - Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation C, F, P DNA metabolic process, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, other, protein binding, ribosome biogenesis 21557 chr12_1 - - 401 C 3 YLR005W SSL1 - Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p C, F, P DNA metabolic process, RNA metabolic process, cell cycle, nucleus, other, response to stress, transcription, transcription regulator activity 21560 chr12_1 - - 401 C 4 YLR007W NSE1 - Essential subunit of the Mms21-Smc5-Smc6 complex; nuclear protein required for DNA repair and growth; has a nonstructural role in the maintenance of chromosomes C, F, P DNA metabolic process, chromosome, molecular_function, nucleus, other, response to stress 21562 chr12_1 - - 401 C 5 YLR008C PAM18 TIM14 J-protein co-chaperone of the mitochondrial import motor associated with the presequence translocase, with Ssc1p, Tim44p, Mge1p, and Pam16p; stimulates ATPase activity of Ssc1p to drive mitochondrial import; activity is inhibited by Pam16p C, F, P cytoplasm, enzyme regulator activity, membrane, mitochondrial envelope, mitochondrion, organelle organization and biogenesis, protein binding, transport, transporter activity 21563 chr12_1 - - 401 C 6 YLR009W RLP24 - Essential protein with similarity to Rpl24Ap and Rpl24Bp, associated with pre-60S ribosomal subunits and required for ribosomal large subunit biogenesis C, F, P molecular_function, nucleolus, organelle organization and biogenesis, ribosome biogenesis 21564 chr12_1 - - 401 C 7 YLR010C TEN1 - Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p C, F, P DNA binding, DNA metabolic process, chromosome, nucleus, organelle organization and biogenesis 21565 chr12_1 - - 401 C 8 YLR022C SDO1 - Essential protein involved in 60S ribosome maturation; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archae and eukaryotes C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis, transport 21577 chr12_1 - - 401 C 9 YLR026C SED5 - cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins C, F, P Golgi apparatus, cytoplasm, membrane organization and biogenesis, organelle organization and biogenesis, protein binding, transport, vesicle-mediated transport 21581 chr12_1 - - 401 C 10 YLR029C RPL15A RPL10A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA C, F, P RNA binding, cytoplasm, ribosome, structural molecule activity, translation 21584 chr12_1 - - 401 C 11 YLR045C STU2 - Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p C, F, P cell cortex, cell cycle, chromosome, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, nucleus, organelle organization and biogenesis, protein binding, structural molecule activity 22656 chr12_2 - - 401 C 12 YLR051C FCF2 - Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon C, F, P RNA metabolic process, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 22662 chr12_2 - - 401 D 1 YLR060W FRS1 - Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; sequence is evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase (Msf1p), but substrate binding is similar C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, translation 22671 chr12_2 - - 401 D 2 YLR066W SPC3 - Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, hydrolase activity, membrane, peptidase activity, protein modification process, transport 22677 chr12_2 - - 401 D 3 YLR071C RGR1 MED14 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 22682 chr12_2 - - 401 D 4 YLR075W RPL10 QSR1|GRC5 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family C, F, P cytoplasm, organelle organization and biogenesis, ribosome, ribosome biogenesis, structural molecule activity, translation 22686 chr12_2 - - 401 D 5 YLR076C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 C, F, P biological_process, cellular_component, molecular_function 22687 chr12_2 - - 401 D 6 YLR078C BOS1 SEC32 v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, membrane, protein binding, transport, vesicle-mediated transport 22689 chr12_2 - - 401 D 7 YLR086W SMC4 - Subunit of the condensin complex, which reorganizes chromosomes during cell division, forms a stable complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity and promotes knotting of circular DNA; potential Cdc28p substrate C, F, P cell cycle, chromosome, hydrolase activity, nucleus, organelle organization and biogenesis, other 22697 chr12_2 - - 401 D 8 YLR088W GAA1 END2 Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, hydrolase activity, lipid metabolic process, membrane, protein modification process 22699 chr12_2 - - 401 D 9 YLR100W ERG27 - 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, lipid metabolic process, membrane, mitochondrial envelope, mitochondrion, oxidoreductase activity 22711 chr12_2 - - 401 D 10 YLR101C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W C, F, P biological_process, cellular_component, molecular_function 22712 chr12_2 - - 401 D 11 YLR103C CDC45 SLD4 DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes C, F, P DNA binding, DNA metabolic process, RNA metabolic process, cell cycle, chromosome, nucleus, organelle organization and biogenesis, other, transcription 22714 chr12_2 - - 401 D 12 YLR105C SEN2 - Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease C, F, P RNA metabolic process, cytoplasm, endomembrane system, hydrolase activity, membrane, mitochondrial envelope, mitochondrion, nucleus 22716 chr12_2 - - 401 E 1 YLR106C MDN1 REA1 Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependent remodeling of 60S subunits and subsequent export from nucleoplasm to cytoplasm C, F, P RNA metabolic process, hydrolase activity, mitochondrion, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 22717 chr12_2 - - 401 E 2 YLR115W CFT2 YDH1 Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. C, F, P RNA binding, RNA metabolic process, nucleus 22726 chr12_2 - - 401 E 3 YLR116W MSL5 - Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA C, F, P RNA binding, RNA metabolic process, nucleus 22727 chr12_2 - - 401 E 4 YLR117C CLF1 NTC77|SYF3 Essential splicesome assembly factor; contains multiple tetratricopeptide repeat (TPR) protein-binding motifs and interacts specifically with many spliceosome components, may serve as a scaffold during splicesome assembly C, F, P DNA metabolic process, RNA metabolic process, chromosome, nucleus, other 22728 chr12_2 - - 401 E 5 YML043C RRN11 - Protein required for rDNA transcription by RNA polymerase I, component of the core factor (CF) of rDNA transcription factor, which also contains Rrn6p and Rrn7p C, F, P RNA metabolic process, nucleolus, nucleus, transcription, transcription regulator activity 20528 chr13_1b - - 401 E 6 YML049C RSE1 - Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport C, F, P RNA binding, RNA metabolic process, nucleus 20522 chr13_1b - - 401 E 7 YML064C TEM1 - GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis C, F, P cell cycle, cytoplasm, cytoskeleton, hydrolase activity, microtubule organizing center, protein binding 20506 chr13_1b - - 401 E 8 YML065W ORC1 - Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity C, F, P DNA binding, DNA metabolic process, RNA metabolic process, chromosome, hydrolase activity, nucleus, organelle organization and biogenesis, other, transcription 20505 chr13_1b - - 401 E 9 YML010W SPT5 - Protein that forms a complex with Spt4p and mediates both activation and inhibition of transcription elongation; Spt4p-Spt5p complex also plays a role in pre-mRNA processing C, F, P DNA metabolic process, RNA metabolic process, mitochondrion, nucleus, organelle organization and biogenesis, transcription, transcription regulator activity, transposition 20564 chr13_2 - - 401 E 10 YML025C YML6 - Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins C, F, P cytoplasm, mitochondrion, organelle organization and biogenesis, ribosome, ribosome biogenesis, structural molecule activity, translation 20548 chr13_2 - - 401 E 11 YMR001C CDC5 PKX2|MSD2 Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate C, F, P DNA metabolic process, cell cycle, cellular bud, cytoskeleton, meiosis, nucleus, protein kinase activity, protein modification process, site of polarized growth 20576 chr13_2 - - 401 E 12 YMR013C SEC59 - Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, membrane, protein modification process, transferase activity 20588 chr13_2 - - 401 F 1 YMR028W TAP42 - Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits C, F, P cellular_component, protein binding, signal transduction 20603 chr13_2 - - 401 F 2 YMR043W MCM1 FUN80 Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes C, F, P DNA binding, DNA metabolic process, RNA metabolic process, chromosome, nucleus, transcription, transcription regulator activity 20619 chr13_2 - - 401 F 3 YMR146C TIF34 - Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation C, F, P cytoplasm, translation, translation regulator activity 20727 chr13_4 - - 401 F 4 YMR149W SWP1 - Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, membrane, protein modification process, transferase activity 20730 chr13_4 - - 401 F 5 YMR168C CEP3 CSL1|CBF3B|CBF3 Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain C, F, P DNA binding, cell cycle, chromosome, cytoskeleton organization and biogenesis, nucleus, organelle organization and biogenesis 20751 chr13_4 - - 401 F 6 YMR197C VTI1 - Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways C, F, P Golgi apparatus, cytoplasm, endomembrane system, membrane, membrane organization and biogenesis, organelle organization and biogenesis, protein binding, transport, vesicle-mediated transport 20783 chr13_4 - - 401 F 7 YMR200W ROT1 - Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations C, F, P cell budding, cell cycle, cell wall organization and biogenesis, cytokinesis, cytoplasm, cytoskeleton organization and biogenesis, endomembrane system, endoplasmic reticulum, membrane, organelle organization and biogenesis, protein binding, protein folding 20786 chr13_4 - - 401 F 8 YMR203W TOM40 MOM38|ISP42 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore C, F, P cytoplasm, membrane, mitochondrial envelope, mitochondrion, organelle organization and biogenesis, transport, transporter activity 20789 chr13_4 - - 401 F 9 YMR208W ERG12 RAR1 Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate C, F, P cytoplasm, lipid metabolic process, transferase activity 20794 chr13_4 - - 401 F 10 YJL061W NUP82 HRB187 Nucleoporin, subunit of the nuclear pore complex (NPC); forms a subcomplex with Nup159p and Nsp1p, interacts with Nup116p and is required for proper localization of Nup116p in the NPC C, F, P endomembrane system, membrane, nuclear organization and biogenesis, nucleus, organelle organization and biogenesis, structural molecule activity, transport 21362 chr10_2 - - 401 F 11 YMR213W CEF1 NTC85 Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p C, F, P RNA metabolic process, nucleus, other 20799 chr13_4 - - 401 F 12 YMR218C TRS130 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1 C, F, P Golgi apparatus, cytoplasm, enzyme regulator activity, signal transduction, transport, vesicle-mediated transport 20804 chr13_4 - - 401 G 1 YMR220W ERG8 - Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate C, F, P cytoplasm, lipid metabolic process, transferase activity 20806 chr13_4 - - 401 G 2 YMR229C RRP5 - RNA binding protein with preference for single stranded tracts of U's involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 20815 chr13_4 - - 401 G 3 YMR235C RNA1 - GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport C, F, P RNA metabolic process, cytoplasm, enzyme regulator activity, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription, transport 20821 chr13_5 - - 401 G 4 YMR236W TAF9 TAF17 Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 C, F, P RNA metabolic process, cell cycle, nucleus, organelle organization and biogenesis, protein modification process, transcription, transcription regulator activity 20822 chr13_5 - - 401 G 5 YMR239C RNT1 - RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end C, F, P RNA metabolic process, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 20825 chr13_5 - - 401 G 6 YMR240C CUS1 - Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p C, F, P RNA binding, RNA metabolic process, nucleus, protein binding 20826 chr13_5 - - 401 G 7 YMR260C TIF11 - Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 C, F, P RNA binding, ribosome, translation, translation regulator activity 20846 chr13_5 - - 401 G 8 YMR268C PRP24 - Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling C, F, P RNA metabolic process, nucleus, other 20854 chr13_5 - - 401 G 9 YMR270C RRN9 - Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I C, F, P DNA binding, RNA metabolic process, nucleolus, nucleus, transcription, transcription regulator activity 20856 chr13_5 - - 401 G 10 YMR281W GPI12 - ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, hydrolase activity, lipid metabolic process, membrane, protein modification process 20867 chr13_5 - - 401 G 11 YMR288W HSH155 - U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 C, F, P RNA binding, RNA metabolic process, nucleus 20874 chr13_5 - - 401 G 12 YMR290C HAS1 - ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles C, F, P RNA binding, RNA metabolic process, cytoplasm, endomembrane system, helicase activity, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 20877 chr13_5 - - 401 H 1 YMR290W-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase C, F, P biological_process, cellular_component, molecular_function 20876 chr13_5 - - 401 H 2 YMR296C LCB1 TSC2|END8 Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, lipid metabolic process, membrane, membrane fraction, transferase activity 20884 chr13_5 met- did not mate 401 H 3 YMR298W LIP1 - Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, lipid metabolic process, membrane, nucleus, transferase activity 20886 chr13_5 - - 401 H 4 YMR301C ATM1 - Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol C, F, P cellular homeostasis, cytoplasm, hydrolase activity, membrane, mitochondrial envelope, mitochondrion, transporter activity 20889 chr13_5 - - 401 H 6 YMR308C PSE1 KAP121 Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p C, F, P cytoplasm, nucleus, protein binding, transport, transporter activity 20898 chr13_5 - - 401 H 7 YMR309C NIP1 - Subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection C, F, P cytoplasm, ribosome biogenesis, translation, translation regulator activity 20899 chr13_5 - - 401 H 8 YOR004W UTP23 - Essential nucleolar protein that is a component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function C, F, P RNA metabolic process, cytoplasm, mitochondrion, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 21780 chr15_1 - - 401 H 9 YOR020C HSP10 CPN10 Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES C, F, P cytoplasm, mitochondrion, protein binding, protein folding 21796 chr15_1 met- did not mate 401 H 10 YOR046C DBP5 RAT8 Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family involved in mRNA export from the nucleus; involved in translation termination C, F, P cytoplasm, endomembrane system, helicase activity, membrane, nucleus, other, translation, transport 21822 chr15_1 - - 401 H 11 YOR048C RAT1 XRN2|TAP1|HKE1 Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as mRNA transcription termination C, F, P RNA metabolic process, hydrolase activity, mitochondrion, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 21824 chr15_1 - - 401 H 12 YOR056C NOB1 YOR29-07 Essential nuclear protein involved in proteasome maturation and synthesis of 40S ribosomal subunits; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleus, organelle organization and biogenesis, other, protein binding, ribosome biogenesis 21832 chr15_1 - - 402 A 1 YOR057W SGT1 YOR29-08 Probable cochaperone, regulates activity of Cyr1p (adenylyl cyclase); involved in assembly of the kinetochore complex, associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex C, F, P cell cycle, organelle organization and biogenesis, other, protein binding, protein modification process, signal transduction 21833 chr15_1 - - 402 A 3 YOR060C - YOR29-11 Protein of unknown function required for cell viability; deletion mutant harbors aberrant mitochondria C, F, P cytoplasm, cytoskeleton, endomembrane system, endoplasmic reticulum, microtubule organizing center, molecular_function, nucleus, organelle organization and biogenesis 21836 chr15_1 - - 402 A 4 YOR063W RPL3 YOR29-14|TCM1|MAK8 Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribosomal proteins; involved in the replication and maintenance of killer double stranded RNA virus C, F, P cytoplasm, organelle organization and biogenesis, ribosome, ribosome biogenesis, structural molecule activity, translation 21839 chr15_1 - - 402 A 5 YOR074C CDC21 YOR29-25|TMP1|CRT9 Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S C, F, P DNA metabolic process, nucleus, other, transferase activity 21850 chr15_1 - - 402 A 6 YOR075W UFE1 YOR29-26 t-SNARE required for ER membrane fusion and vesicular traffic, integral membrane protein that constitutes with Sec20p and Use1p the trimeric acceptor for R/v-SNAREs on Golgi-derived vesicles at the ER; part of Dsl1p complex C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, membrane, membrane organization and biogenesis, organelle organization and biogenesis, protein binding, transport, vesicle-mediated transport 21851 chr15_1 - - 402 A 7 YOR077W RTS2 YOR29-28 Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication C, F, P biological_process, cytoplasm, molecular_function, nucleus 21853 chr15_1 - - 402 A 8 YOR095C RKI1 - Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis C, F, P carbohydrate metabolic process, cofactor metabolic process, cytoplasm, isomerase activity, nucleus, ribosome biogenesis, vitamin metabolic process 21871 chr15_1 - - 402 A 9 YOR294W RRS1 - Essential protein that binds ribosomal protein L11 and is required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; mouse homolog shows altered expression in Huntington's disease model mice C, F, P RNA metabolic process, molecular_function, nucleus, organelle organization and biogenesis, ribosome biogenesis 21590 chr15_4 - - 402 A 10 YOR310C NOP58 NOP5 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 21607 chr15_4 met- did not mate 402 A 11 YJL072C PSF2 CDC102 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery C, F, P DNA metabolic process, chromosome, molecular_function, nucleus 21351 chr10_2 - - 402 A 12 YOR326W MYO2 CDC66 One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle C, F, P cell budding, cell cycle, cellular bud, cytokinesis, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, membrane, membrane organization and biogenesis, motor activity, organelle organization and biogenesis, other, protein binding, site of polarized growth, transport, vacuole, vesicle-mediated transport 21623 chr15_4 - - 402 B 1 YOR329C SCD5 FTB1 Protein required for normal cortical actin organization and endocytosis; multicopy suppressor of clathrin deficiency; acts as a targeting subunit for protein phosphatase type 1 C, F, P cell cortex, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, membrane fraction, membrane organization and biogenesis, organelle organization and biogenesis, protein binding, transport, vesicle-mediated transport 21626 chr15_4 - - 402 B 2 YOR335C ALA1 CDC64 Cytoplasmic alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, mitochondrion, translation 21632 chr15_4 - - 402 B 3 YJL069C UTP18 - Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 21354 chr10_2 - - 402 B 4 YOR341W RPA190 RRN1 RNA polymerase I subunit; largest subunit of RNA polymerase I C, F, P RNA metabolic process, nucleolus, nucleotidyltransferase activity, nucleus, transcription, transferase activity 21638 chr15_4 - - 402 B 5 YOR353C SOG2 - Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis C, F, P anatomical structure morphogenesis, cellular bud, cytokinesis, cytoplasm, molecular_function, other, signal transduction, site of polarized growth 21650 chr15_4 - - 402 B 6 YOR361C PRT1 DNA26|CDC63 Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes C, F, P cytoplasm, translation, translation regulator activity 21658 chr15_4 - - 402 B 7 YOR362C PRE10 - Alpha 7 subunit of the 20S proteasome C, F, P cytoplasm, hydrolase activity, other, peptidase activity, protein catabolic process 21659 chr15_4 - - 402 B 8 YOR370C MRS6 MSI4 Rab escort protein, forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) C, F, P cytoplasm, enzyme regulator activity, membrane, protein binding, transport 21667 chr15_4 - - 402 B 9 YOR372C NDD1 - Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes C, F, P RNA metabolic process, cell cycle, nucleus, protein binding, transcription, transcription regulator activity 21669 chr15_4 - - 402 B 10 YOR373W NUD1 - Component of the spindle pole body outer plaque, required for exit from mitosis C, F, P cell cycle, cytoplasm, cytoskeleton, meiosis, microtubule organizing center, structural molecule activity 21670 chr15_4 - - 402 B 11 YOR336W KRE5 - Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects C, F, P carbohydrate metabolic process, cell wall organization and biogenesis, endoplasmic reticulum, transferase activity 21633 chr15_4 - - 402 B 12 YOL005C RPB11 - RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit C, F, P RNA metabolic process, nucleotidyltransferase activity, nucleus, transcription, transferase activity 21696 chr15_5 - - 402 C 1 YOL010W RCL1 - RNA terminal phosphate cyclase-like protein involved in rRNA processing at sites A0, A1, and A2; does not possess detectable RNA cyclase activity C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 21701 chr15_5 - - 402 C 2 YOL021C DIS3 RRP44 Catalytic component of the exosome, involved in RNA processing and degradation; binds Gsp1p/Ran and enhances the GEF activity of Srm1p; implicated in mitotic control; homologous to the E. coli RNase R of the RNase II family C, F, P RNA metabolic process, cytoplasm, hydrolase activity, mitochondrion, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 21712 chr15_5 - - 402 C 3 YOL022C - - Cytoplasmic protein of unknown function; essential gene in S288C, and non-essential with reduced growth rate in CEN.PK2; Null mutant accumulates 20S pre-rRNA C, F, P biological_process, cytoplasm, molecular_function 21713 chr15_5 - - 402 C 4 YOL026C MIM1 TOM13 Mitochondrial outer membrane protein, required for assembly of the translocase of the outer membrane (TOM) complex and thereby for mitochondrial protein import; N terminus is exposed to the cytosol: transmembrane segment is highly conserved C, F, P Golgi apparatus, cytoplasm, endomembrane system, membrane, mitochondrial envelope, mitochondrion, molecular_function, organelle organization and biogenesis, other, transport 21717 chr15_5 - - 402 C 5 YOL034W SMC5 - Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair C, F, P DNA metabolic process, chromosome, molecular_function, nucleus, other, response to stress 21725 chr15_5 - - 402 C 6 YOL038W PRE6 - Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress C, F, P cytoplasm, hydrolase activity, mitochondrion, other, peptidase activity, protein catabolic process 21729 chr15_5 - - 402 C 7 YOL040C RPS15 RPS21 Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ribosomal proteins C, F, P cytoplasm, organelle organization and biogenesis, ribosome, ribosome biogenesis, structural molecule activity, translation, transport 21731 chr15_5 - - 402 C 8 YOL066C RIB2 PUS8 DRAP deaminase, catalyzes the third step of the riboflavin biosynthesis pathway; cytoplasmic tRNA pseudouridine synthase involved in pseudouridylation of cytoplasmic tRNAs at position 32 C, F, P RNA metabolic process, cytoplasm, isomerase activity, other, vitamin metabolic process 21758 chr15_5 - - 402 C 9 YOL069W NUF2 - Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering C, F, P chromosome, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, nucleus, organelle organization and biogenesis, other, structural molecule activity 21761 chr15_5 - - 402 C 10 YOL077C BRX1 - Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif C, F, P RNA binding, nucleolus, organelle organization and biogenesis, ribosome biogenesis 21768 chr15_5 - - 402 C 11 YPL190C NAB3 HMD1 Single stranded RNA binding protein; acidic ribonucleoprotein; required for termination of non-poly(A) transcripts and efficient splicing; interacts with Nrd1p C, F, P RNA binding, RNA metabolic process, nucleus, transcription 21102 chr16_1 - - 402 C 12 YPL204W HRR25 KTI14 Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) C, F, P DNA metabolic process, cell cycle, cellular bud, chromosome, cytoskeleton organization and biogenesis, meiosis, nucleus, organelle organization and biogenesis, plasma membrane, protein kinase activity, protein modification process, response to stress, ribosome biogenesis, site of polarized growth, transferase activity, transport, vesicle-mediated transport 21088 chr16_1 - - 402 D 1 YPL209C IPL1 PAC15 Aurora kinase involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; associates with Sli15p, which promotes Ipl1p association with mitotic spindle C, F, P cell cycle, chromosome, cytokinesis, cytoskeleton, cytoskeleton organization and biogenesis, meiosis, nucleus, organelle organization and biogenesis, other, protein kinase activity 21083 chr16_1 - - 402 D 2 YPL210C SRP72 - Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane C, F, P cytoplasm, other, transport 21082 chr16_1 - - 402 D 3 YPL211W NIP7 - Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, organelle organization and biogenesis, ribosome, ribosome biogenesis 21081 chr16_1 - - 402 D 4 YPL218W SAR1 - GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport C, F, P Golgi apparatus, cytoplasm, cytoplasmic membrane-bounded vesicle, endomembrane system, hydrolase activity, membrane, transport, vesicle-mediated transport 21074 chr16_1 - - 402 D 5 YPL228W CET1 CES5 Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide C, F, P RNA metabolic process, hydrolase activity, nucleus 21064 chr16_1 - - 402 D 6 YPL231W FAS2 - Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities; phosphorylated C, F, P cytoplasm, lipid metabolic process, mitochondrion, oxidoreductase activity, transferase activity 21061 chr16_1 - - 402 D 7 YPL233W NSL1 - Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation C, F, P chromosome, cytoskeleton, molecular_function, nucleus, other 21059 chr16_1 - - 402 D 8 YPL235W RVB2 TIP49|TIH2|TIP49B Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family C, F, P RNA metabolic process, helicase activity, hydrolase activity, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 21057 chr16_1 - - 402 D 9 YPL237W SUI3 - Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding C, F, P cytoplasm, ribosome, translation, translation regulator activity 21054 chr16_1 - - 402 D 10 YPL238C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W C, F, P biological_process, cellular_component, molecular_function 21055 chr16_1 - - 402 D 11 YPL242C IQG1 CYK1 Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family C, F, P cell cortex, cellular bud, cytokinesis, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, organelle organization and biogenesis, protein binding, response to stress, site of polarized growth 21050 chr16_1 - - 402 D 12 YPL243W SRP68 - Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane C, F, P cytoplasm, other, transport 21049 chr16_1 - - 402 E 1 YPL251W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C C, F, P biological_process, cellular_component, molecular_function 21040 chr16_1 - - 402 E 2 YPL252C YAH1 - Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin C, F, P cofactor metabolic process, cytoplasm, heterocycle metabolic process, mitochondrion, oxidoreductase activity 21041 chr16_1 - - 402 E 3 YPL255W BBP1 - Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p C, F, P cell cycle, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, organelle organization and biogenesis, structural molecule activity 21037 chr16_1 - - 402 E 4 YPL266W DIM1 CDH1 Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing C, F, P RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transferase activity 21026 chr16_1 - - 402 E 5 YPL093W NOG1 - Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins C, F, P RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis, transport 22159 chr16_2 - - 402 E 6 YPL094C SEC62 LPG14 Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, membrane, protein binding, transport, transporter activity 22158 chr16_2 - - 402 E 7 YPL117C IDI1 LPH10|BOT2 Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability C, F, P cytoplasm, isomerase activity, lipid metabolic process 22135 chr16_2 - - 402 E 8 YPL122C TFB2 - Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH C, F, P DNA metabolic process, RNA metabolic process, cell cycle, nucleus, other, response to stress, transcription, transcription regulator activity 22130 chr16_2 - - 402 E 9 YPL124W SPC29 NIP29|LPH3 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication C, F, P cell cycle, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, organelle organization and biogenesis, structural molecule activity 22128 chr16_2 - - 402 E 10 YPL126W NAN1 UTP17 U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA C, F, P RNA binding, RNA metabolic process, chromosome, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 22126 chr16_2 - - 402 E 11 YPL128C TBF1 LPI16 Telobox-containing general regulatory factor; binds to TTAGGG repeats within subtelomeric anti-silencing regions (STARs) and possibly throughout the genome and mediates their insulating capacity by blocking silent chromatin propagation C, F, P DNA binding, RNA metabolic process, chromosome, nucleus, organelle organization and biogenesis, transcription, transcription regulator activity 22124 chr16_2 - - 402 E 12 YPL142C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit C, F, P biological_process, cellular_component, molecular_function 22110 chr16_2 - - 402 F 1 YPL143W RPL33A RPL37A N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Bp and has similarity to rat L35a; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable C, F, P cytoplasm, ribosome, structural molecule activity, translation 22109 chr16_2 - - 402 F 2 YPL151C PRP46 NTC50 Splicing factor that is found in the Cef1p subcomplex of the spliceosome C, F, P RNA metabolic process, molecular_function, nucleus 22101 chr16_2 - - 402 F 3 YPL153C RAD53 SPK1|MEC2|LSD1 Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication C, F, P DNA binding, DNA metabolic process, nucleus, other, protein kinase activity, response to stress, transferase activity 22099 chr16_2 - - 402 F 4 YPL160W CDC60 - Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, translation 22092 chr16_2 - - 402 F 5 YPL169C MEX67 - Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP C, F, P RNA binding, cytoplasm, endomembrane system, membrane, nucleus, organelle organization and biogenesis, protein binding, ribosome biogenesis, structural molecule activity, transport 22083 chr16_2 - - 402 F 6 YPL175W SPT14 GPI3|CWH6 UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins C, F, P cytoplasm, endoplasmic reticulum, lipid metabolic process, protein modification process, transferase activity 22077 chr16_2 - - 402 F 7 YBR190W - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W C, F, P biological_process, cellular_component, molecular_function 23330 chr2_4 - - 402 F 8 YBR192W RIM2 PYT1 Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family C, F, P mitochondrion, organelle organization and biogenesis, transport, transporter activity 23332 chr2_4 - - 402 F 9 YBR193C MED8 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 23333 chr2_4 - - 402 F 10 YBR196C PGI1 CDC30 Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation C, F, P carbohydrate metabolic process, cofactor metabolic process, cytoplasm, generation of precursor metabolites and energy, isomerase activity, mitochondrion, vitamin metabolic process 23336 chr2_4 - - 402 F 11 YBR198C TAF5 TAF90 Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification C, F, P RNA metabolic process, cell cycle, nucleus, organelle organization and biogenesis, protein modification process, transcription, transcription regulator activity 23338 chr2_4 - - 402 F 12 YBR202W MCM7 CDC47 Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase C, F, P DNA binding, DNA metabolic process, RNA metabolic process, cell cycle, chromosome, cytoplasm, helicase activity, hydrolase activity, nucleus, other, protein binding, transcription, transcription regulator activity 23342 chr2_4 - - 402 G 1 YBR211C AME1 ARP100 Essential kinetochore protein associated with microtubules and spindle pole bodies; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance C, F, P chromosome, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, molecular_function, nucleus, organelle organization and biogenesis 23351 chr2_4 - - 402 G 2 YBR234C ARC40 - Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches C, F, P cytoskeleton, cytoskeleton organization and biogenesis, organelle organization and biogenesis, structural molecule activity 23374 chr2_4 - - 402 G 3 YBR236C ABD1 - Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA C, F, P RNA metabolic process, nucleus, transferase activity 23376 chr2_4 - - 402 G 4 YBR237W PRP5 RNA5 RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA C, F, P RNA metabolic process, helicase activity, hydrolase activity, nucleus, other 23377 chr2_4 - - 402 G 5 YBR243C ALG7 TUR1 UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin C, F, P cellular respiration, endoplasmic reticulum, generation of precursor metabolites and energy, protein modification process, transferase activity 23383 chr2_4 - - 402 G 6 YBR247C ENP1 MEG1 Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 23387 chr2_4 - - 402 G 7 YBR252W DUT1 - dUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability C, F, P cytoplasm, hydrolase activity, nucleus, other 23392 chr2_4 - - 402 G 8 YBR253W SRB6 MED22 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C, F, P RNA metabolic process, nucleus, structural molecule activity, transcription, transcription regulator activity 23393 chr2_4 met- did not mate 402 G 9 YBR254C TRS20 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder C, F, P Golgi apparatus, cytoplasm, enzyme regulator activity, transport, vesicle-mediated transport 23394 chr2_4 - - 402 G 10 YBR256C RIB5 - Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway C, F, P other, transferase activity, vitamin metabolic process 23396 chr2_4 - - 402 G 11 YBR257W POP4 - Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in RNase P C, F, P RNA metabolic process, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 23397 chr2_4 - - 402 G 12 YBR265W TSC10 - 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family C, F, P cytoplasm, endoplasmic reticulum, lipid metabolic process, membrane, mitochondrial envelope, mitochondrion, oxidoreductase activity 23405 chr2_4 - - 402 H 1 YBR189W RPS9B SUP46|RPS13A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins C, F, P cytoplasm, nucleolus, nucleus, ribosome, structural molecule activity, translation 23329 chr2_4 - - 402 H 2 YBR191W RPL21A URP1 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein C, F, P cytoplasm, ribosome, structural molecule activity, translation 23331 chr2_4 - - 402 H 3 YDR052C DBF4 DNA52|LSD7 Regulatory subunit of Cdc7p-Dbf4p kinase complex, required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated C, F, P DNA binding, DNA metabolic process, enzyme regulator activity, other 23988 chr4_4 - - 402 H 4 YDR053W - - Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex C, F, P biological_process, cellular_component, molecular_function 23989 chr4_4 - - 402 H 6 YDR054C CDC34 UBC3|DNA6 Ubiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation C, F, P cell cycle, cytoplasm, ligase activity, nucleus, other, protein binding, protein catabolic process, protein modification process 23990 chr4_4 - - 402 H 7 YDR060W MAK21 NOC1 Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein C, F, P cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 23995 chr4_4 - - 402 H 8 YDR062W LCB2 TSC1|SCS1 Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, lipid metabolic process, membrane, membrane fraction, transferase activity 23997 chr4_4 - - 402 H 9 YDR064W RPS13 RPS13C|RPS13B Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins C, F, P cytoplasm, nucleolus, nucleus, ribosome, structural molecule activity, translation 23999 chr4_4 - - 402 H 10 YDR081C PDC2 - Transcription factor required for the synthesis of the glycolytic enzyme pyruvate decarboxylase, required for high level expression of both the THI and the PDC genes C, F, P RNA metabolic process, generation of precursor metabolites and energy, nucleus, transcription, transcription regulator activity, vitamin metabolic process 24016 chr4_4 - - 402 H 11 YDR082W STN1 - Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping C, F, P DNA binding, DNA metabolic process, chromosome, nucleus, organelle organization and biogenesis 24017 chr4_4 - - 402 H 12 YDR086C SSS1 - Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, membrane, protein binding, transport, transporter activity 24021 chr4_4 - - 403 A 1 YDR087C RRP1 - Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles C, F, P RNA metabolic process, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24022 chr4_4 - - 403 A 2 YDR088C SLU7 SLT17 RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain C, F, P RNA metabolic process, nucleus, other 24023 chr4_4 met- did not mate 403 A 4 YDR091C RLI1 - Essential iron-sulfur protein required for ribosome biogenesis and translation initiation; facilitates binding of a multifactor complex (MFC) of translation initiation factors to the small ribosomal subunit; predicted ABC family ATPase C, F, P cytoplasm, hydrolase activity, nucleus, organelle organization and biogenesis, other, ribosome, ribosome biogenesis, translation, transport 24026 chr4_4 - - 403 A 5 YDR113C PDS1 - Securin that inhibits anaphase by binding separin Esp1p, also blocks cyclin destruction and mitotic exit, essential for cell cycle arrest in mitosis in the presence of DNA damage or aberrant mitotic spindles; also present in meiotic nuclei C, F, P DNA metabolic process, cell cycle, cytoskeleton, nucleus, organelle organization and biogenesis, other, protein binding, response to stress 24047 chr4_4 - - 403 A 6 YDR118W APC4 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition C, F, P cell cycle, cytoskeleton organization and biogenesis, ligase activity, nucleus, organelle organization and biogenesis, protein binding, protein catabolic process, protein modification process 24052 chr4_4 - - 403 A 7 YDR141C DOP1 - Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C, F, P anatomical structure morphogenesis, cytoplasm, mitochondrion, molecular_function, organelle organization and biogenesis, transport, vesicle-mediated transport 24075 chr4_4 - - 403 A 8 YDR145W TAF12 TAF68|TAF61 Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A C, F, P RNA metabolic process, cell cycle, nucleus, organelle organization and biogenesis, protein modification process, transcription, transcription regulator activity 24079 chr4_4 met- did not mate 403 A 9 YDR339C FCF1 UTP24 Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24178 chr4_7 - - 403 A 10 YDR341C - RRS1 Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, mitochondrion, translation 24180 chr4_7 - - 403 A 11 YDR353W TRR1 - Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress C, F, P cellular homeostasis, cytoplasm, oxidoreductase activity, response to chemical stimulus, response to stress 24190 chr4_7 - - 403 A 12 YDR355C - - Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W C, F, P biological_process, cellular_component, molecular_function 24192 chr4_7 - - 403 B 1 YDR356W SPC110 NUF1|XCM1 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner C, F, P conjugation, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, nuclear organization and biogenesis, organelle organization and biogenesis, structural molecule activity 24193 chr4_7 - - 403 B 2 YDR361C BCP1 - Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport C, F, P cytoplasm, molecular_function, nucleus, organelle organization and biogenesis, ribosome biogenesis, transport 24198 chr4_7 met- did not mate 403 B 3 YDR362C TFC6 - One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 24199 chr4_7 - - 403 B 4 YDR365C ESF1 - Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels C, F, P RNA binding, RNA metabolic process, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24202 chr4_7 - - 403 B 5 YDR367W - - Component of the inositol phosphorylceramide synthase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C, F, P Golgi apparatus, biological_process, cytoplasm, molecular_function 24203 chr4_7 - - 403 B 6 YDR373W FRQ1 - N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily C, F, P enzyme regulator activity, membrane, other, signal transduction 24209 chr4_7 - - 403 B 7 YDR376W ARH1 - Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability C, F, P cellular homeostasis, cytoplasm, membrane, mitochondrial envelope, mitochondrion, oxidoreductase activity 24212 chr4_7 - - 403 B 8 YDR381W YRA1 SHE11 Nuclear protein that binds to RNA and to Mex67p, required for export of poly(A)+ mRNA from the nucleus; member of the REF (RNA and export factor binding proteins) family; another family member, Yra2p, can substitute for Yra1p function C, F, P DNA metabolic process, RNA binding, nucleus, response to stress, transport 24217 chr4_7 - - 403 B 9 YDR390C UBA2 UAL1 Nuclear protein that acts as a heterodimer with Aos1p to activate Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability C, F, P nucleus, other, protein modification process 24226 chr4_7 - - 403 B 10 YDR394W RPT3 YTA2|YNT1 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B C, F, P hydrolase activity, other, peptidase activity, protein catabolic process 24230 chr4_7 - - 403 B 11 YDR396W - - Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex C, F, P biological_process, cellular_component, molecular_function 24232 chr4_7 - - 403 B 12 YDR397C NCB2 YDR1 Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta C, F, P RNA metabolic process, nucleus, protein binding, transcription, transcription regulator activity 24233 chr4_7 - - 403 C 1 YDR398W UTP5 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C, F, P RNA binding, RNA metabolic process, chromosome, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 24234 chr4_7 - - 403 C 2 YDR404C RPB7 - RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p C, F, P RNA metabolic process, cytoplasm, nucleotidyltransferase activity, nucleus, transcription, transferase activity 24240 chr4_7 - - 403 C 3 YDR407C TRS120 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic C, F, P Golgi apparatus, cytoplasm, molecular_function, signal transduction, transport, vesicle-mediated transport 24243 chr4_7 - - 403 C 4 YDR412W RRP17 - Component of the pre-60S pre-ribosomal particle; required for cell viability C, F, P RNA metabolic process, cytoplasm, molecular_function, organelle organization and biogenesis, ribosome biogenesis 24248 chr4_7 - - 403 C 5 YDR413C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing C, F, P biological_process, cellular_component, molecular_function 24249 chr4_7 met- did not mate 403 C 6 YDR416W SYF1 NTC90 Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression; similar to Drosophila crooked neck protein C, F, P RNA metabolic process, cell cycle, nucleus, other 24252 chr4_7 - - 403 C 7 YDR429C TIF35 - Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation C, F, P cytoplasm, translation, translation regulator activity 24265 chr4_7 - - 403 C 8 YDR434W GPI17 - Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Sp homolog C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, hydrolase activity, lipid metabolic process, membrane, protein modification process 24270 chr4_7 - - 403 C 9 YDR427W RPN9 - Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects C, F, P cytoplasm, other, protein catabolic process, structural molecule activity 24263 chr4_7 - - 403 C 10 YEL002C WBP1 - Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum C, F, P cell cycle, cytoplasm, endomembrane system, endoplasmic reticulum, membrane, nucleus, protein modification process, transferase activity 20242 chr5_2 - - 403 C 11 YEL019C MMS21 NSE2 SUMO ligase involved in chromosomal organization and DNA repair; essential subunit of the Mms21-Smc5-Smc6 complex; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination C, F, P DNA metabolic process, chromosome, cytoplasm, endomembrane system, ligase activity, nucleus, response to stress 20260 chr5_2 - - 403 C 12 YEL026W SNU13 - RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 20267 chr5_2 - - 403 D 1 YEL032W MCM3 - Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex C, F, P DNA binding, DNA metabolic process, cell cycle, chromosome, cytoplasm, hydrolase activity, nucleus, other 20273 chr5_2 - - 403 D 2 YEL035C UTR5 - Protein of unknown function; transcription may be regulated by Gcr1p C, F, P biological_process, cellular_component, molecular_function 20276 chr5_2 - - 403 D 3 YEL055C POL5 - DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA C, F, P RNA metabolic process, nucleolus, nucleotidyltransferase activity, transcription, transferase activity 20296 chr5_2 - - 403 D 4 YEL058W PCM1 AGM1 Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors C, F, P carbohydrate metabolic process, cell wall organization and biogenesis, cytoplasm, isomerase activity, nucleus 20299 chr5_2 - - 403 D 5 YER003C PMI40 PMI Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation C, F, P carbohydrate metabolic process, cell wall organization and biogenesis, cytoplasm, isomerase activity, nucleus, protein modification process 20324 chr5_2 - - 403 D 6 YER006W NUG1 - GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus C, F, P RNA metabolic process, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 20327 chr5_2 - - 403 D 7 YER009W NTF2 - Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport C, F, P endomembrane system, nucleus, protein binding, transport 20331 chr5_2 - - 403 D 8 YER012W PRE1 - Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle C, F, P hydrolase activity, nucleus, other, peptidase activity, protein catabolic process, response to stress, sporulation 20334 chr5_2 - - 403 D 9 YER013W PRP22 - DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes C, F, P RNA metabolic process, helicase activity, hydrolase activity, nucleus, other 20335 chr5_2 - - 403 D 10 YER021W RPN3 SUN2 Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control C, F, P molecular_function, other, protein catabolic process 20153 chr5_3 - - 403 D 11 YER022W SRB4 MED17 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 20154 chr5_3 - - 403 D 12 YER023W PRO3 ORE2 Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis C, F, P amino acid and derivative metabolic process, cytoplasm, oxidoreductase activity 20155 chr5_3 - - 403 E 1 YER025W GCD11 SUI4 Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met C, F, P cytoplasm, ribosome, translation, translation regulator activity 20157 chr5_3 - - 403 E 2 YER036C ARB1 - ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p C, F, P cytoplasm, hydrolase activity, nucleus, organelle organization and biogenesis, ribosome, ribosome biogenesis, transport 20168 chr5_3 - - 403 E 3 YER038C KRE29 - Essential subunit of the Mms21-Smc5-Smc6 complex; protein of unknown function; required for growth and DNA repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance C, F, P DNA metabolic process, chromosome, cytoplasm, molecular_function, nucleus, response to stress 20170 chr5_3 met- did not mate 403 E 4 YER043C SAH1 - S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor C, F, P amino acid and derivative metabolic process, cytoplasm, hydrolase activity 20176 chr5_3 - - 403 E 5 YER082C UTP7 KRE31 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 20222 chr5_3 - - 403 E 6 YGR128C UTP8 - Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C, F, P RNA binding, RNA metabolic process, chromosome, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 24758 chr7_5 - - 403 E 7 YGR140W CBF2 CSL5|NDC10|CTF14|CEP2|CBF3A Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo C, F, P DNA binding, cell cycle, chromosome, cytoskeleton, cytoskeleton organization and biogenesis, nucleus, organelle organization and biogenesis, other 24770 chr7_5 - - 403 E 8 YGR147C NAT2 - Protein with an apparent role in acetylation of N-terminal methionine residues C, F, P mitochondrion, molecular_function, protein modification process 24777 chr7_5 - - 403 E 9 YGR156W PTI1 - Pta1p Interacting protein C, F, P RNA binding, RNA metabolic process, nucleus, transcription 24786 chr7_5 - - 403 E 10 YGR158C MTR3 - 3'5' exoribonuclease, exosome subunit; nucleolar protein involved in export of mRNA and ribosomal subunits; homologous to the E. coli exonuclease RNase PH C, F, P RNA metabolic process, cytoplasm, hydrolase activity, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 24788 chr7_5 - - 403 E 11 YGR172C YIP1 - Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles C, F, P Golgi apparatus, cytoplasm, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, membrane, membrane organization and biogenesis, molecular_function, organelle organization and biogenesis, transport, vesicle-mediated transport 24802 chr7_5 - - 403 E 12 YGR175C ERG1 - Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine C, F, P cytoplasm, endoplasmic reticulum, lipid metabolic process, other, oxidoreductase activity 24805 chr7_5 - - 403 F 1 YGR179C OKP1 - Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly C, F, P chromosome, nucleus, other, protein binding 24809 chr7_5 - - 403 F 2 YGR185C TYS1 TyrRS|TTS1 Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, nucleus, translation 24815 chr7_5 - - 403 F 3 YGR186W TFG1 RAP74|SSU71 TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 24816 chr7_5 - - 403 F 4 YGR190C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W C, F, P biological_process, cellular_component, molecular_function 24820 chr7_5 - - 403 F 5 YGR191W HIP1 - High-affinity histidine permease, also involved in the transport of manganese ions C, F, P plasma membrane, transport, transporter activity 24821 chr7_5 - - 403 F 6 YGR195W SKI6 RRP41|ECM20 3'-to-5' phosphorolytic exoribonuclease that is a subunit of the exosome; required for 3' processing of the 5.8S rRNA; involved in 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs C, F, P RNA metabolic process, cytoplasm, hydrolase activity, nucleus, organelle organization and biogenesis, other, response to chemical stimulus, ribosome biogenesis 24825 chr7_5 - - 403 F 7 YGR198W YPP1 - Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene C, F, P cell cortex, cytoplasm, cytoskeleton, membrane organization and biogenesis, molecular_function, plasma membrane, response to chemical stimulus, ribosome, signal transduction, transport, vesicle-mediated transport 24828 chr7_5 - - 403 F 8 YGR211W ZPR1 - Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p) C, F, P cell cycle, cytoplasm, nucleus, protein binding, response to stress 24841 chr7_5 - - 403 F 9 YGR216C GPI1 - Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs C, F, P cytoplasm, endoplasmic reticulum, lipid metabolic process, membrane, protein modification process, transferase activity 24846 chr7_5 - - 403 F 10 YGR218W CRM1 XPO1|KAP124 Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus C, F, P nucleus, organelle organization and biogenesis, protein binding, ribosome biogenesis, transport, transporter activity 24848 chr7_5 - - 403 F 11 YHR019C DED81 - Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, other, translation 20982 chr8_1 - - 403 F 12 YHR024C MAS2 MIF2 Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins C, F, P cytoplasm, hydrolase activity, mitochondrion, organelle organization and biogenesis, peptidase activity, protein modification process, transport 20987 chr8_1 - - 403 G 1 YHR036W BRL1 - Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export C, F, P endomembrane system, endoplasmic reticulum, membrane organization and biogenesis, molecular_function, nuclear organization and biogenesis, nucleus, organelle organization and biogenesis, transport 20999 chr8_1 - - 403 G 2 YHR040W BCD1 - Essential protein required for the accumulation of box C/D snoRNA C, F, P RNA metabolic process, cytoplasm, nucleus, other 21003 chr8_1 met- did not mate 403 G 3 YHR042W NCP1 CPR1 NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p C, F, P cytoplasm, lipid metabolic process, membrane, membrane fraction, mitochondrial envelope, mitochondrion, other 21005 chr8_1 - - 403 G 4 YHR062C RPP1 - Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends C, F, P RNA metabolic process, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 21890 chr8_2 - - 403 G 5 YHR065C RRP3 - Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity C, F, P RNA metabolic process, helicase activity, hydrolase activity, nucleolus, organelle organization and biogenesis, ribosome biogenesis 21893 chr8_2 - - 403 G 6 YHR068W DYS1 - Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric C, F, P amino acid and derivative metabolic process, cytoplasm, protein modification process, transferase activity 21896 chr8_2 - - 403 G 7 YHR069C RRP4 - 3'-5' exoribonuclease involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp43p and Dis3p C, F, P RNA binding, RNA metabolic process, cytoplasm, hydrolase activity, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 21897 chr8_2 - - 403 G 8 YHR070W TRM5 - tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya C, F, P RNA metabolic process, cytoplasm, mitochondrion, nucleus, ribosome biogenesis, transferase activity 21898 chr8_2 - - 403 G 9 YHR072W ERG7 - Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis C, F, P cytoplasm, endoplasmic reticulum, isomerase activity, lipid metabolic process, plasma membrane 21900 chr8_2 - - 403 G 10 YHR074W QNS1 - Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide C, F, P cofactor metabolic process, cytoplasm, hydrolase activity, ligase activity, nucleus, vitamin metabolic process 21902 chr8_2 - - 403 G 11 YHR083W SAM35 TOM38|TOB38|FMP20 Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane C, F, P cytoplasm, membrane, membrane organization and biogenesis, mitochondrial envelope, mitochondrion, organelle organization and biogenesis, protein binding, transport 21911 chr8_2 - - 403 G 12 YHR085W IPI1 - Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles C, F, P RNA metabolic process, molecular_function, nucleus, organelle organization and biogenesis, ribosome biogenesis 21913 chr8_2 - - 403 H 1 YHR088W RPF1 - Nucleolar protein involved in the assembly of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA C, F, P RNA binding, RNA metabolic process, nucleolus, organelle organization and biogenesis, ribosome biogenesis 21916 chr8_2 - - 403 H 2 YHR101C BIG1 - Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan C, F, P cell wall organization and biogenesis, cytoplasm, endomembrane system, endoplasmic reticulum, membrane, molecular_function 21929 chr8_2 - - 403 H 3 YHR107C CDC12 PSL7|CLA10 Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM C, F, P anatomical structure morphogenesis, cell budding, cell cortex, cell wall organization and biogenesis, cellular bud, conjugation, cytokinesis, cytoplasm, cytoskeleton, hydrolase activity, lipid binding, site of polarized growth, structural molecule activity 21935 chr8_2 - - 403 H 4 YHR118C ORC6 - Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p C, F, P DNA binding, DNA metabolic process, RNA metabolic process, chromosome, nucleus, organelle organization and biogenesis, transcription 21946 chr8_2 - - 403 H 6 YHR122W - - Protein of unknown function required for establishment of sister chromatid cohesion; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; may be involved in transcription; YHR122W is an essential gene C, F, P cell cycle, cytoplasm, molecular_function, nucleus, organelle organization and biogenesis, transcription 21950 chr8_2 - - 403 H 7 YHR084W STE12 - Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth C, F, P DNA binding, RNA metabolic process, conjugation, nucleus, other, pseudohyphal growth, response to chemical stimulus, transcription, transcription regulator activity 21912 chr8_2 - - 403 H 8 YHR143W-A RPC10 RPB12 RNA polymerase subunit, found in RNA polymerase complexes I, II, and III C, F, P RNA metabolic process, nucleolus, nucleotidyltransferase activity, nucleus, transcription, transferase activity 22837 chr8_3 - - 403 H 9 YHR148W IMP3 - Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 22842 chr8_3 - - 403 H 10 YHR164C DNA2 WEB2 Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate C, F, P DNA metabolic process, chromosome, helicase activity, hydrolase activity, nucleus, organelle organization and biogenesis, other, response to stress 22858 chr8_3 - - 403 H 11 YHR166C CDC23 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition C, F, P cell cycle, cytoskeleton organization and biogenesis, ligase activity, nucleus, organelle organization and biogenesis, protein binding, protein catabolic process, protein modification process 22860 chr8_3 met- did not mate 403 H 12 YHR172W SPC97 - Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque C, F, P cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, organelle organization and biogenesis, structural molecule activity 22866 chr8_3 - - 404 A 1 YHR186C KOG1 LAS24 Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors C, F, P cytoplasm, membrane, membrane fraction, mitochondrion, molecular_function, other, plasma membrane, response to stress, signal transduction, vacuole 22880 chr8_3 - - 404 A 2 YHR188C GPI16 - Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, hydrolase activity, lipid metabolic process, membrane, protein modification process 22882 chr8_3 - - 404 A 3 YHR190W ERG9 - Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway C, F, P cytoplasm, endoplasmic reticulum, lipid metabolic process, membrane, mitochondrial envelope, mitochondrion, transferase activity 22884 chr8_3 - - 404 A 5 YHR196W UTP9 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C, F, P RNA binding, RNA metabolic process, chromosome, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transcription 22890 chr8_3 - - 404 A 6 YHR197W RIX1 IPI2 Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles C, F, P RNA metabolic process, molecular_function, nucleus, organelle organization and biogenesis, ribosome biogenesis, transport 22891 chr8_3 - - 404 A 7 YCL003W - - - - - 23411 chr3_1 - - 404 A 8 YCL004W PGS1 YCL003W|PEL1 Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis C, F, P lipid metabolic process, mitochondrion, transferase activity 23412 chr3_1 - - 404 A 9 YCL017C NFS1 SPL1 Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria C, F, P RNA metabolic process, cellular homeostasis, cofactor metabolic process, lyase activity, mitochondrion, nucleus, transferase activity 23424 chr3_1 - - 404 A 10 YCL031C RRP7 - Essential protein involved in rRNA processing and ribosome biogenesis C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 23438 chr3_1 - - 404 A 11 YCL043C PDI1 TRG1|MFP1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds C, F, P cytoplasm, endoplasmic reticulum, isomerase activity, oxidoreductase activity, protein folding 23450 chr3_1 - - 404 A 12 YCL052C PBN1 - Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X C, F, P endoplasmic reticulum, lipid metabolic process, protein catabolic process, protein modification process, transferase activity 23459 chr3_1 - - 404 B 1 YCL054W SPB1 - AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants C, F, P RNA metabolic process, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis, transferase activity 23461 chr3_1 - - 404 B 2 YCL059C KRR1 - Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 23466 chr3_1 met- did not mate 404 B 3 YCR012W PGK1 - 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis C, F, P carbohydrate metabolic process, cytoplasm, generation of precursor metabolites and energy, mitochondrion, transferase activity 23492 chr3_1 - - 404 B 4 YCR013C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant C, F, P biological_process, cellular_component, molecular_function 23493 chr3_1 - - 404 B 5 YLR127C APC2 TID2|RSI1 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p C, F, P cell cycle, cytoskeleton organization and biogenesis, ligase activity, nucleus, organelle organization and biogenesis, protein binding, protein catabolic process, protein modification process 24084 chr12_3 - - 404 B 6 YLR129W DIP2 UTP12 Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24086 chr12_3 - - 404 B 7 YLR132C - - Protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene C, F, P RNA metabolic process, cellular respiration, cytoplasm, generation of precursor metabolites and energy, mitochondrion, molecular_function, nucleus 24089 chr12_3 - - 404 B 8 YLR140W - - Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I C, F, P biological_process, cellular_component, molecular_function 24097 chr12_3 - - 404 B 9 YLR141W RRN5 - Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p C, F, P DNA binding, RNA metabolic process, nucleolus, nucleus, transcription, transcription regulator activity 24098 chr12_3 - - 404 B 10 YLR145W RMP1 - Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P C, F, P RNA metabolic process, cytoplasm, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 24102 chr12_3 - - 404 B 11 YLR147C SMD3 SLT16 Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 C, F, P RNA binding, RNA metabolic process, nucleus, other 24104 chr12_3 - - 404 B 12 YLR163C MAS1 MIF1 Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins C, F, P cytoplasm, hydrolase activity, mitochondrion, organelle organization and biogenesis, peptidase activity, protein modification process, transport 24112 chr12_3 - - 404 C 1 YLR166C SEC10 - Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis C, F, P anatomical structure morphogenesis, cell budding, cell cortex, cytokinesis, cytoplasm, membrane organization and biogenesis, organelle organization and biogenesis, protein binding, transport, vesicle-mediated transport 24115 chr12_3 - - 404 C 2 YLR167W RPS31 UBI3|RPS37 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B C, F, P cytoplasm, organelle organization and biogenesis, other, protein modification process, ribosome, ribosome biogenesis, structural molecule activity, translation 24116 chr12_3 - - 404 C 3 YLR175W CBF5 - Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2 C, F, P RNA metabolic process, cytoplasm, isomerase activity, lyase activity, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24124 chr12_3 - - 404 C 4 YLR186W EMG1 NEP1 Member of the alpha/beta knot fold methyltransferase superfamily; required for maturation of 18S rRNA and for 40S ribosome production; interacts with RNA and with S-adenosylmethionine; associates with spindle/microtubules; forms homodimers C, F, P RNA binding, RNA metabolic process, cytoplasm, cytoskeleton, nucleolus, nucleus, organelle organization and biogenesis, protein binding, ribosome biogenesis 24135 chr12_3 - - 404 C 5 YLR195C NMT1 CDC72 N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction C, F, P cytoplasm, other, protein modification process, transferase activity 24144 chr12_3 - - 404 C 6 YLR196W PWP1 - Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24145 chr12_3 - - 404 C 7 YLR197W SIK1 NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24146 chr12_3 - - 404 C 8 YLR198C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W C, F, P biological_process, cellular_component, molecular_function 24147 chr12_3 - - 404 C 9 YLR208W SEC13 ANU3 Component of both the Nup84 nuclear pore sub-complex and of the COPII complex (Sar1p, Sec13p, Sec16p, Sec23p, Sec24p, Sec31p, Sfb2p, and Sfb3p) which is important for the formation of ER to Golgi transport vesicles C, F, P Golgi apparatus, cytoplasm, cytoplasmic membrane-bounded vesicle, endomembrane system, membrane, membrane organization and biogenesis, nuclear organization and biogenesis, nucleus, organelle organization and biogenesis, protein catabolic process, structural molecule activity, transport, vesicle-mediated transport 24157 chr12_3 - - 404 C 10 YLR212C TUB4 - Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body C, F, P cell cycle, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, organelle organization and biogenesis, structural molecule activity 24161 chr12_3 - - 404 C 11 YLR222C UTP13 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24171 chr12_3 - - 404 C 12 YLR223C IFH1 - Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate C, F, P DNA binding, RNA metabolic process, nucleolus, nucleus, organelle organization and biogenesis, transcription, transcription regulator activity 24172 chr12_3 - - 404 D 1 YKL004W AUR1 - Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance C, F, P Golgi apparatus, lipid metabolic process, transferase activity 24852 chr11_1 - - 404 D 2 YKL006C-A SFT1 - Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment C, F, P Golgi apparatus, cytoplasm, endomembrane system, membrane, protein binding, transport, vesicle-mediated transport 24854 chr11_1 - - 404 D 3 YKL012W PRP40 - U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex C, F, P RNA binding, RNA metabolic process, nucleus 24861 chr11_1 - - 404 D 4 YKL013C ARC19 - Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches C, F, P cytoskeleton, cytoskeleton organization and biogenesis, organelle organization and biogenesis, structural molecule activity 24862 chr11_1 - - 404 D 5 YKL014C URB1 NPA1 Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit C, F, P RNA metabolic process, molecular_function, nucleolus, organelle organization and biogenesis, ribosome biogenesis 24863 chr11_1 - - 404 D 6 YKL018W SWD2 SAF37|CPS35 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination C, F, P RNA metabolic process, nucleus, organelle organization and biogenesis, protein modification process, transcription, transferase activity 24867 chr11_1 - - 404 D 7 YKL019W RAM2 - Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor C, F, P cytoplasm, other, protein modification process, transferase activity 24868 chr11_1 - - 404 D 8 YKL021C MAK11 - Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats C, F, P RNA metabolic process, membrane fraction, molecular_function, organelle organization and biogenesis, ribosome biogenesis 24870 chr11_1 - - 404 D 9 YKL022C CDC16 - Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation C, F, P DNA metabolic process, cell cycle, cytoskeleton organization and biogenesis, ligase activity, nucleus, organelle organization and biogenesis, protein binding, protein catabolic process, protein modification process 24871 chr11_1 - - 404 D 10 YKL024C URA6 SOC8 Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) C, F, P aromatic compound metabolic process, cytoplasm, heterocycle metabolic process, nucleus, other, transferase activity 24873 chr11_1 - - 404 D 11 YKL028W TFA1 - TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening C, F, P RNA metabolic process, mitochondrion, nucleus, transcription, transcription regulator activity 24877 chr11_1 - - 404 D 12 YKL033W - FMP47 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YKL033W is an essential gene C, F, P biological_process, cytoplasm, mitochondrion, molecular_function 24882 chr11_1 - - 404 E 1 YKL035W UGP1 - UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p C, F, P carbohydrate metabolic process, cell wall organization and biogenesis, cytoplasm, generation of precursor metabolites and energy, nucleotidyltransferase activity, protein modification process, transferase activity 24884 chr11_1 - - 404 E 2 YKL036C - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W C, F, P biological_process, cellular_component, molecular_function 24885 chr11_1 - - 404 E 3 YKL042W SPC42 - Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane C, F, P cell cycle, cytoplasm, cytoskeleton, cytoskeleton organization and biogenesis, microtubule organizing center, organelle organization and biogenesis, structural molecule activity 24891 chr11_1 - - 404 E 4 YKL045W PRI2 - Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair C, F, P DNA metabolic process, RNA metabolic process, chromosome, endomembrane system, nucleotidyltransferase activity, nucleus, response to stress, transferase activity 24894 chr11_1 - - 404 E 5 YKL049C CSE4 CSL2 Centromere protein that resembles histones, required for proper kinetochore function; homolog of human CENP-A C, F, P DNA binding, cell cycle, chromosome, nucleus, organelle organization and biogenesis, other 24898 chr11_1 - - 404 E 6 YKL052C ASK1 - Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases C, F, P cell cycle, chromosome, cytoskeleton, cytoskeleton organization and biogenesis, nucleus, organelle organization and biogenesis, other, structural molecule activity 24901 chr11_1 - - 404 E 7 YKL058W TOA2 - TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 24907 chr11_1 - - 404 E 8 YKL059C MPE1 - Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif C, F, P RNA binding, RNA metabolic process, nucleus 24908 chr11_1 - - 404 E 9 YKL060C FBA1 LOT1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress C, F, P carbohydrate metabolic process, cytoplasm, generation of precursor metabolites and energy, lyase activity, mitochondrion 24909 chr11_1 - - 404 E 10 YKL078W DHR2 - Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis C, F, P helicase activity, nucleolus, ribosome biogenesis 24927 chr11_1 - - 404 E 11 YKL083W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 C, F, P biological_process, cellular_component, molecular_function 24932 chr11_1 - - 404 E 12 YKL088W - - Predicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p C, F, P cofactor metabolic process, cytoplasm, lyase activity, other, response to stress 24937 chr11_1 - - 404 F 1 YKL089W MIF2 - Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p C, F, P DNA binding, cell cycle, chromosome, cytoskeleton organization and biogenesis, nucleus, organelle organization and biogenesis, other 24938 chr11_1 - - 404 F 2 YKL095W YJU2 CWC16 Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing C, F, P RNA metabolic process, molecular_function, nucleus 24944 chr11_1 met- did not mate 404 F 3 YKL099C UTP11 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C, F, P RNA binding, RNA metabolic process, cytoplasm, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24949 chr11_2 - - 404 F 4 YKL104C GFA1 - Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis C, F, P carbohydrate metabolic process, cell wall organization and biogenesis, cellular_component, transferase activity 24954 chr11_2 - - 404 F 5 YKL108W SLD2 DRC1 Protein required for DNA replication, phosphorylated in S phase by S-phase cyclin-dependent kinases (Cdks), phosphorylation is essential for DNA replication and for complex formation with Dpb11p; potential Cdc28p substrate C, F, P DNA metabolic process, chromosome, molecular_function, nucleus 24958 chr11_2 - - 404 F 6 YKL111C - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 C, F, P biological_process, cellular_component, molecular_function 24961 chr11_2 - - 404 F 7 YKL112W ABF1 SBF1|REB2|OBF1|BAF1 DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair C, F, P DNA binding, DNA metabolic process, RNA metabolic process, chromosome, nucleus, organelle organization and biogenesis, other, response to stress, transcription, transcription regulator activity 24962 chr11_2 - - 404 F 8 YKL122C SRP21 - Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm C, F, P cytoplasm, nucleus, other, transport 24972 chr11_2 - - 404 F 9 YKL125W RRN3 - Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA C, F, P RNA metabolic process, nucleolus, nucleus, transcription, transcription regulator activity 24975 chr11_2 - - 404 F 10 YKL144C RPC25 YKL1 RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p C, F, P RNA metabolic process, nucleotidyltransferase activity, nucleus, transcription, transferase activity 24994 chr11_2 - - 404 F 11 YKL145W RPT1 YTA3|CIM5 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p C, F, P hydrolase activity, other, peptidase activity, protein catabolic process 24995 chr11_2 - - 404 F 12 YKL152C GPM1 - Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis C, F, P carbohydrate metabolic process, cytoplasm, generation of precursor metabolites and energy, isomerase activity, mitochondrion 25002 chr11_2 - - 404 G 1 YKL153W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant C, F, P biological_process, cellular_component, molecular_function 25003 chr11_2 - - 404 G 2 YKL154W SRP102 - Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors Srp101p to the ER membrane C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, hydrolase activity, membrane, other, transport 25004 chr11_2 - - 404 G 3 YKL165C MCD4 FSR2|SSU21|ZRG16 Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes C, F, P cell wall, cytoplasm, endoplasmic reticulum, lipid metabolic process, protein modification process, transferase activity, transport, vacuole 25015 chr11_2 - - 404 G 4 YKL172W EBP2 - Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles C, F, P RNA metabolic process, molecular_function, nucleolus, organelle organization and biogenesis, ribosome biogenesis 25022 chr11_2 - - 404 G 5 YKL173W SNU114 GIN10 GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 C, F, P RNA binding, RNA metabolic process, hydrolase activity, nucleus, other 25023 chr11_2 - - 404 G 6 YKL180W RPL17A RPL17 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the Dam1 complex (aka DASH complex) C, F, P cytoplasm, ribosome, structural molecule activity, translation 25030 chr11_2 - - 404 G 7 YKL186C MTR2 - mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA C, F, P endomembrane system, membrane, nucleus, organelle organization and biogenesis, protein binding, ribosome biogenesis, transport 25036 chr11_2 - - 404 G 8 YKL189W HYM1 - Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response C, F, P anatomical structure morphogenesis, cellular bud, cytokinesis, molecular_function, other, site of polarized growth, transcription 25039 chr11_2 - - 404 G 9 YKL139W CTK1 - Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing C, F, P RNA metabolic process, nucleus, protein kinase activity, protein modification process, transcription, transferase activity 24989 chr11_2 - - 404 G 10 YKL141W SDH3 YKL4|CYB3 Cytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone C, F, P cellular respiration, cofactor metabolic process, cytoplasm, generation of precursor metabolites and energy, membrane, mitochondrial envelope, mitochondrion, oxidoreductase activity 24991 chr11_2 - - 404 G 11 YKL181W PRS1 PRP1 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes C, F, P carbohydrate metabolic process, cell wall organization and biogenesis, cytoplasm, transferase activity 25031 chr11_2 - - 404 G 12 YKL182W FAS1 - Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities C, F, P cytoplasm, lipid metabolic process, lyase activity, mitochondrion, oxidoreductase activity, transferase activity 25032 chr11_2 - - 404 H 1 YGR029W ERV1 - Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) C, F, P cellular homeostasis, cytoplasm, mitochondrial envelope, mitochondrion, organelle organization and biogenesis, oxidoreductase activity, response to chemical stimulus, response to stress, transport 24659 chr7_4 - - 404 H 2 YGR030C POP6 - Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends C, F, P RNA metabolic process, hydrolase activity, nucleolus, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 24660 chr7_4 - - 404 H 3 YGR047C TFC4 PCF1 One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 C, F, P RNA metabolic process, nucleus, transcription, transcription regulator activity 24677 chr7_4 - - 404 H 4 YGR060W ERG25 - C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol C, F, P cytoplasm, endomembrane system, endoplasmic reticulum, lipid metabolic process, membrane, oxidoreductase activity, plasma membrane 24690 chr7_4 - - 404 H 6 YGR065C VHT1 - High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin C, F, P plasma membrane, transport, transporter activity 24695 chr7_4 - - 404 H 7 YGR073C - - Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein C, F, P biological_process, cellular_component, molecular_function 24703 chr7_4 - - 404 H 8 YGR074W SMD1 SPP92 Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1 C, F, P RNA binding, RNA metabolic process, nucleus, other 24704 chr7_4 - - 404 H 9 YGR075C PRP38 - Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly C, F, P RNA metabolic process, nucleus, other 24705 chr7_4 - - 404 H 10 YGR083C GCD2 GCD12 Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression C, F, P cytoplasm, enzyme regulator activity, other, translation, translation regulator activity 24713 chr7_4 met- did not mate 404 H 11 YGR090W UTP22 - Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data C, F, P RNA metabolic process, cytoplasm, molecular_function, nucleolus, nucleus, organelle organization and biogenesis, ribosome biogenesis 24720 chr7_4 - - 404 H 12 YGR091W PRP31 - Splicing factor, component of the U4/U6-U5 snRNP complex C, F, P RNA metabolic process, mitochondrion, nucleus, other 24721 chr7_4 - - 405 A 1 YGR094W VAS1 - Mitochondrial and cytoplasmic valyl-tRNA synthetase C, F, P RNA metabolic process, amino acid and derivative metabolic process, cytoplasm, ligase activity, mitochondrion, translation 24724 chr7_4 - - 405 A 2 YGR095C RRP46 - Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex C, F, P RNA metabolic process, cytoplasm, hydrolase activity, nucleus, organelle organization and biogenesis, other, ribosome biogenesis 24725 chr7_4 - - 405 A 3 YGR098C ESP1 - Separase, a caspase-like cysteine protease that promotes sister chromatid separ