mat a Synced to SGD on Wed Feb 1 10:00:10 2012 Plate # Row Column ORF Gene Alias Description GO Biological Process GO Molecular Function GO Cellular Component background [00-1] A 1 - - - - - - - - [00-1] A 2 YAL064C-A TDA8 YAL065C-A Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] A 3 - - - - - - - - [00-1] A 4 YBL091C-A SCS22 - Protein involved in regulation of phospholipid metabolism; homolog of Scs2p; similar to D. melanogaster inturned protein lipid metabolic process molecular_function cellular_component BY4742 [00-1] A 5 YBR269C FMP21 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-1] A 6 - - - - - - - - [00-1] A 7 YBR271W - - S-adenosylmethionine-dependent methyltransferase; belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis biological_process methyltransferase activity cytoplasm BY4742 [00-1] A 8 - - - - - - - - [00-1] A 9 YBR273C UBX7 CUI3 UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p sporulation, proteolysis involved in cellular protein catabolic process molecular_function nucleus, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-1] A 10 YBR274W CHK1 - Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase response to DNA damage stimulus, protein phosphorylation, DNA replication, regulation of DNA metabolic process, regulation of cell cycle kinase activity nucleus BY4742 [00-1] A 11 - - - - - - - - [00-1] A 12 - - - - - - - - [00-1] B 1 YBR277C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W biological_process molecular_function cellular_component BY4742 [00-1] B 2 YBR278W DPB3 - Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle response to DNA damage stimulus, DNA repair, DNA replication DNA binding, nucleotidyltransferase activity nucleus, chromosome BY4742 [00-1] B 3 YBR279W PAF1 - Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 transcription from RNA polymerase I promoter, peptidyl-amino acid modification, mitotic cell cycle, snoRNA processing, chromatin organization, response to DNA damage stimulus, DNA recombination, transcription elongation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter, DNA repair, protein alkylation, protein phosphorylation, rRNA processing, regulation of organelle organization, histone modification, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process nucleic acid binding transcription factor activity, enzyme binding, protein binding transcription factor activity nucleus, chromosome BY4742 [00-1] B 4 - - - - - - - - [00-1] B 5 YBR281C DUG2 - Probable di- and tri-peptidase; forms a complex with Dug1p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) cellular amino acid metabolic process peptidase activity nucleus, cytoplasm BY4742 [00-1] B 6 YBR282W MRPL27 YmL27 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-1] B 7 YBR283C SSH1 - Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential protein targeting protein transporter activity, transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-1] B 8 YBR284W - - Putative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin and synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component BY4742 [00-1] B 9 YBR285W - - Putative protein of unknown function; YBR285W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] B 10 YBR286W APE3 APY1 Vacuolar aminopeptidase Y, processed to mature form by Prb1p other peptidase activity vacuole, cytoplasm BY4742 [00-1] B 11 - - - - - - - - [00-1] B 12 - - - - - - - - [00-1] C 1 - - - - - - - - [00-1] C 2 YBR290W BSD2 - Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification protein targeting, ion transport, proteolysis involved in cellular protein catabolic process other vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] C 3 YBR291C CTP1 - Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-1] C 4 YBR292C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] C 5 YBR293W VBA2 - Permease of basic amino acids in the vacuolar membrane transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-1] C 6 - - - - - - - - [00-1] C 7 YBR295W PCA1 PAY2|CAD2 Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function transmembrane transport, ion transport, cellular ion homeostasis transmembrane transporter activity, ion binding, ATPase activity membrane, plasma membrane BY4742 [00-1] C 8 YBR296C PHO89 ITN1 Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-1] C 9 YBR297W MAL33 MALR|MAL3R MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C carbohydrate metabolic process nucleic acid binding transcription factor activity nucleus BY4742 [00-1] C 10 YBR298C MAL31 MALT|MAL3T Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, membrane fraction BY4742 [00-1] C 11 YBR300C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] C 12 - - - - - - - - [00-1] D 1 YCL001W-A - - Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III biological_process molecular_function cellular_component BY4742 [00-1] D 2 - - - - - - - - [00-1] D 3 YCR020W-B HTL1 - Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance response to DNA damage stimulus, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, regulation of cell cycle other nucleus BY4742 [00-1] D 4 YCR024C SLM5 - Mitochondrial asparaginyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-1] D 5 YCR024C-A PMP1 - Small single-membrane span proteolipid that functions as a regulatory subunit of the plasma membrane H(+)-ATPase Pma1p, forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines ion transport enzyme regulator activity membrane, plasma membrane BY4742 [00-1] D 6 YCR025C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] D 7 YCR026C NPP1 - Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation response to starvation, nucleobase-containing small molecule metabolic process nuclease activity cellular_component BY4742 [00-1] D 8 YCR027C RHB1 RSG1 Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins endosome transport, amino acid transport GTPase activity membrane, plasma membrane BY4742 [00-1] D 9 YCR028C FEN2 - Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph membrane invagination, transmembrane transport, amino acid transport, endocytosis transmembrane transporter activity membrane, plasma membrane BY4742 [00-1] D 10 - - - - - - - - [00-1] D 11 - - - - - - - - [00-1] D 12 YCR031C RPS14A rp59A|S14A|RPL59|CRY1 Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity, mRNA binding ribosome, cytoplasm BY4742 [00-1] E 1 - - - - - - - - [00-1] E 2 - - - - - - - - [00-1] E 3 YCR034W FEN1 VBM2|GNS1|ELO2 Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway lipid metabolic process transferase activity, transferring acyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-1] E 4 - - - - - - - - [00-1] E 5 YCR036W RBK1 - Putative ribokinase carbohydrate metabolic process kinase activity nucleus, cytoplasm BY4742 [00-1] E 6 YCR037C PHO87 - Low-affinity inorganic phosphate (Pi) transporter, involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments transmembrane transport, ion transport transmembrane transporter activity membrane BY4742 [00-1] E 7 YCR043C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene biological_process molecular_function site of polarized growth, cytoplasm, Golgi apparatus BY4742 [00-1] E 8 YCR044C PER1 COS16 Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 cellular ion homeostasis, lipid metabolic process, protein lipidation molecular_function vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] E 9 YCR045C RRT12 OSW3 Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores sporulation, cell wall organization or biogenesis peptidase activity cell wall, nucleus, endomembrane system BY4742 [00-1] E 10 - - - - - - - - [00-1] E 11 - - - - - - - - [00-1] E 12 - - - - - - - - [00-1] F 1 YCR049C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-1] F 2 YCR050C - - Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component BY4742 [00-1] F 3 YCR051W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [00-1] F 4 - - - - - - - - [00-1] F 5 - - - - - - - - [00-1] F 6 - - - - - - - - [00-1] F 7 - - - - - - - - [00-1] F 8 YCR059C YIH1 - Protein that inhibits activation of Gcn2p, an eIF2 alpha subunit protein kinase, by competing for Gcn1p binding, thus impacting gene expression in response to starvation; has sequence and functional similarity to the mouse IMPACT gene cellular amino acid metabolic process molecular_function nucleus, cytoplasm BY4742 [00-1] F 9 - - - - - - - - [00-1] F 10 YCR061W - YCR062W Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation other molecular_function membrane, cytoplasm BY4742 [00-1] F 11 - - - - - - - - [00-1] F 12 YCR063W BUD31 CWC14 Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern RNA splicing, cytokinesis, mRNA processing molecular_function nucleus BY4742 [00-1] G 1 - - - - - - - - [00-1] G 2 YCR065W HCM1 - Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role mitotic cell cycle, mitochondrion organization, cytoskeleton organization, response to oxidative stress, response to chemical stimulus, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome, cytoplasm BY4742 [00-1] G 3 YCR066W RAD18 - E3 ubiqutin ligase, forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair DNA repair, response to DNA damage stimulus, protein modification by small protein conjugation or removal ligase activity, DNA binding, ATPase activity nucleus, chromosome BY4742 [00-1] G 4 - - - - - - - - [00-1] G 5 YCR068W ATG15 CVT17|AUT5 Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway membrane invagination, protein maturation other membrane, vacuole, cytoplasm BY4742 [00-1] G 6 - - - - - - - - [00-1] G 7 YCR071C IMG2 - Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-1] G 8 - - - - - - - - [00-1] G 9 - - - - - - - - [00-1] G 10 YCR073W-A SOL2 YCRX13W Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol1p, Sol3p, and Sol4p nucleobase-containing compound transport, nuclear transport molecular_function cytoplasm BY4742 [00-1] G 11 - - - - - - - - [00-1] G 12 YCR076C - - Putative protein of unknown function; YCR076C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] H 1 YCR077C PAT1 MRT1 Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p RNA catabolic process, translational initiation, regulation of translation, chromosome segregation molecular_function ribosome, cytoplasm BY4742 [00-1] H 2 YCR079W PTC6 PPP2|AUP1 Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase protein dephosphorylation, response to starvation phosphatase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-1] H 3 YCR081W SRB8 MED12|YCR080W|SSN5|NUT6|GIG1 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression transcription from RNA polymerase II promoter, response to chemical stimulus protein binding transcription factor activity nucleus BY4742 [00-1] H 4 YCR082W AHC2 - Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups nucleus, cytoplasm BY4742 [00-1] H 5 - - - - - - - - [00-1] H 6 - - - - - - - - [00-1] H 7 YCR085W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-1] H 8 YCR086W CSM1 - Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation mitotic cell cycle, meiotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, endomembrane system, chromosome, nucleolus BY4742 [00-1] H 9 YCR087C-A - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene biological_process molecular_function nucleus, nucleolus BY4742 [00-1] H 10 YCR087W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene biological_process molecular_function membrane BY4742 [00-1] H 11 - - - - - - - - [00-1] H 12 - - - - - - - - [00-2] A 1 YCR090C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [00-2] A 2 YCR091W KIN82 FPK2 Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p lipid transport, protein phosphorylation, response to chemical stimulus kinase activity cellular_component BY4742 [00-2] A 3 YCR092C MSH3 - Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination DNA binding nucleus, cytoplasm BY4742 [00-2] A 4 - - - - - - - - [00-2] A 5 YCR094W CDC50 - Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Ynr048wp and Lem3p lipid transport, membrane invagination, endocytosis ATPase activity membrane fraction, cytoplasm, Golgi apparatus BY4742 [00-2] A 6 - - - - - - - - [00-2] A 7 YCR098C GIT1 - Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-2] A 8 YCR099C - - Putative protein of unknown function biological_process molecular_function membrane BY4742 [00-2] A 9 YCR100C - - Putative protein of unknown function biological_process molecular_function membrane BY4742 [00-2] A 10 YCR101C - - Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene biological_process molecular_function membrane, membrane fraction BY4742 [00-2] A 11 YCR102C - - Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to chemical stimulus molecular_function cellular_component BY4742 [00-2] A 12 - - - - - - - - [00-2] B 1 YCR105W ADH7 ADHVII NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance other oxidoreductase activity other BY4742 [00-2] B 2 YCR106W RDS1 - Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide response to chemical stimulus DNA binding nucleus BY4742 [00-2] B 3 YDL130W-A STF1 AIS2 Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p may act as stabilizing factors that enhance inhibitory action of the Inh1p protein nucleobase-containing small molecule metabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] B 4 - - - - - - - - [00-2] B 5 - - - - - - - - [00-2] B 6 - - - - - - - - [00-2] B 7 - - - - - - - - [00-2] B 8 YDR363W-A SEM1 HOD1|DSS1 Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1 proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, protein complex biogenesis, exocytosis, nuclear transport, regulation of cell cycle molecular_function nucleus, membrane fraction, cytoplasm BY4742 [00-2] B 9 YDR525W-A SNA2 - Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-2] B 10 YDR535C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. biological_process molecular_function cellular_component BY4742 [00-2] B 11 YDR536W STL1 - Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-2] B 12 - - - - - - - - [00-2] C 1 YDR538W PAD1 POF1 Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX other lyase activity, RNA binding, mRNA binding mitochondrion, cytoplasm BY4742 [00-2] C 2 YDR539W FDC1 - Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm other molecular_function cytoplasm BY4742 [00-2] C 3 YDR540C IRC4 - Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus DNA recombination molecular_function nucleus, cytoplasm BY4742 [00-2] C 4 YDR541C - - Putative dihydrokaempferol 4-reductase biological_process oxidoreductase activity cellular_component BY4742 [00-2] C 5 - - - - - - - - [00-2] C 6 YER039C-A - - Putative protein of unknown function; YER039C-A is not an essential gene biological_process molecular_function membrane BY4742 [00-2] C 7 - - - - - - - - [00-2] C 8 - - - - - - - - [00-2] C 9 YER091C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-2] C 10 YER144C UBP5 - Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck protein modification by small protein conjugation or removal peptidase activity cellular bud, site of polarized growth BY4742 [00-2] C 11 YER188W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p biological_process molecular_function cellular_component BY4742 [00-2] C 12 YFL034C-A RPL22B rp4|l1c|YL31|L22B|YFL035C-B Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Ap and to rat L22 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-2] D 1 YFR032C RRT5 - Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation biological_process molecular_function cellular_component BY4742 [00-2] D 2 YFR032C-A RPL29 YL43|L29 Protein component of the large (60S) ribosomal subunit, has similarity to rat L29 ribosomal protein; not essential for translation, but required for proper joining of the large and small ribosomal subunits and for normal translation rate cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-2] D 3 YFR033C QCR6 UCR6|COR3 Subunit 6 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1 generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-2] D 4 YFR034C PHO4 phoD Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability chromatin organization, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-2] D 5 YFR035C - - Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component BY4742 [00-2] D 6 YFR036W CDC26 SCD26|HIT3 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus BY4742 [00-2] D 7 - - - - - - - - [00-2] D 8 YFR038W IRC5 - Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci DNA recombination molecular_function cellular_component BY4742 [00-2] D 9 - - - - - - - - [00-2] D 10 YFR040W SAP155 - Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p mitotic cell cycle phosphatase activity cytoplasm BY4742 [00-2] D 11 YFR041C ERJ5 - Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response protein folding molecular_function endoplasmic reticulum, cytoplasm BY4742 [00-2] D 12 - - - - - - - - [00-2] E 1 YFR043C IRC6 - Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component BY4742 [00-2] E 2 YFR044C DUG1 - Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) cellular amino acid metabolic process peptidase activity ribosome, mitochondrion, cytoplasm BY4742 [00-2] E 3 YFR045W - - Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white other other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] E 4 YFR046C CNN1 - Kinetochore protein of unknown function; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by both Clb5-Cdk1 and, to a lesser extent, Clb2-Cdk1. chromosome segregation molecular_function nucleus, chromosome BY4742 [00-2] E 5 YFR047C BNA6 QPT1 Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm BY4742 [00-2] E 6 YFR048W RMD8 - Cytosolic protein required for sporulation biological_process molecular_function cytoplasm BY4742 [00-2] E 7 YFR049W YMR31 - Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-2] E 8 - - - - - - - - [00-2] E 9 - - - - - - - - [00-2] E 10 - - - - - - - - [00-2] E 11 YFR053C HXK1 - Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p carbohydrate metabolic process, carbohydrate transport, generation of precursor metabolites and energy kinase activity mitochondrion, cytoplasm BY4742 [00-2] E 12 YFR054C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-2] F 1 YFR055W IRC7 - Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner cellular amino acid metabolic process lyase activity cellular_component BY4742 [00-2] F 2 YFR056C - - Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W biological_process molecular_function cellular_component BY4742 [00-2] F 3 YFR057W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-2] F 4 YGR219W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C biological_process molecular_function cellular_component BY4742 [00-2] F 5 YGR220C MRPL9 YmL9 Mitochondrial ribosomal protein of the large subunit cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-2] F 6 YGR221C TOS2 - Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p cell budding molecular_function cellular bud, site of polarized growth BY4742 [00-2] F 7 YGR222W PET54 - Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing mitochondrial translation, RNA splicing, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis RNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-2] F 8 YGR223C HSV2 - Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization membrane invagination lipid binding membrane fraction, vacuole, cytoplasm BY4742 [00-2] F 9 YGR224W AZR1 - Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole response to chemical stimulus transmembrane transporter activity membrane, plasma membrane BY4742 [00-2] F 10 YGR225W AMA1 SPO70 Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis meiotic cell cycle, sporulation, cell wall organization or biogenesis enzyme regulator activity nucleus BY4742 [00-2] F 11 YGR226C - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W biological_process molecular_function cellular_component BY4742 [00-2] F 12 YGR227W DIE2 ALG10 Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 carbohydrate metabolic process, protein glycosylation, oligosaccharide metabolic process transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-2] G 1 YGR228W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C biological_process molecular_function membrane BY4742 [00-2] G 2 YGR229C SMI1 KNR4 Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity mitotic cell cycle, cell wall organization or biogenesis, regulation of cell cycle molecular_function cellular bud, site of polarized growth BY4742 [00-2] G 3 YGR230W BNS1 - Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis meiotic cell cycle molecular_function cellular_component BY4742 [00-2] G 4 YGR231C PHB2 - Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation protein folding, organelle inheritance, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] G 5 YGR232W NAS6 - Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [00-2] G 6 YGR233C PHO81 VAC6|phoS Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p other enzyme regulator activity nucleus, cytoplasm BY4742 [00-2] G 7 YGR234W YHB1 YHB4 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses other oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-2] G 8 YGR235C MOS2 MCS29|MIO27 Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] G 9 YGR236C SPG1 - Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-2] G 10 YGR237C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-2] G 11 - - - - - - - - [00-2] G 12 - - - - - - - - [00-2] H 1 YGR240C PFK1 - Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes carbohydrate metabolic process, generation of precursor metabolites and energy kinase activity mitochondrion, cytoplasm BY4742 [00-2] H 2 YGR241C YAP1802 - Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1801p, member of the AP180 protein family membrane invagination, endocytosis other cell cortex, cytoskeleton, cytoplasm BY4742 [00-2] H 3 YGR242W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C biological_process molecular_function ribosome, cytoplasm BY4742 [00-2] H 4 YGR243W FMP43 - Putative protein of unknown function; expression regulated by osmotic and alkaline stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-2] H 5 YGR244C LSC2 - Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration ligase activity mitochondrion, cytoplasm BY4742 [00-2] H 6 - - - - - - - - [00-2] H 7 - - - - - - - - [00-2] H 8 YGR247W CPD1 - Cyclic nucleotide phosphodiesterase, hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression nucleobase-containing small molecule metabolic process other cytoplasmic membrane-bounded vesicle, cytoplasm BY4742 [00-2] H 9 - - - - - - - - [00-2] H 10 YGR249W MGA1 - Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants other nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-2] H 11 - - - - - - - - [00-2] H 12 - - - - - - - - [00-3] A 1 - - - - - - - - [00-3] A 2 - - - - - - - - [00-3] A 3 - - - - - - - - [00-3] A 4 YGR256W GND2 - 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity membrane fraction, cytoplasm BY4742 [00-3] A 5 - - - - - - - - [00-3] A 6 - - - - - - - - [00-3] A 7 YGR259C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W biological_process molecular_function cellular_component BY4742 [00-3] A 8 YGR260W TNA1 - High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) nucleobase-containing compound transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-3] A 9 YGR261C APL6 YKS5 Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Golgi vesicle transport molecular_function membrane, cytoplasm BY4742 [00-3] A 10 YGR262C BUD32 LDB14 Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription telomere organization, protein phosphorylation, cytokinesis, nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter kinase activity nucleus, cytoplasm BY4742 [00-3] A 11 YGR263C SAY1 - Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum lipid metabolic process, response to chemical stimulus other membrane, endoplasmic reticulum, cytoplasm BY4742 [00-3] A 12 - - - - - - - - [00-3] B 1 - - - - - - - - [00-3] B 2 YGR266W - - Protein of unknown function, predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm BY4742 [00-3] B 3 - - - - - - - - [00-3] B 4 YGR268C HUA1 - Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm BY4742 [00-3] B 5 YGR269W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C biological_process molecular_function cellular_component BY4742 [00-3] B 6 YGR270W YTA7 - Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p transcription from RNA polymerase II promoter histone binding nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-3] B 7 - - - - - - - - [00-3] B 8 - - - - - - - - [00-3] B 9 - - - - - - - - [00-3] B 10 - - - - - - - - [00-3] B 11 YGR275W RTT102 - Component of both the SWI/SNF and RSC chromatin remodeling complexes, suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, chromosome segregation molecular_function nucleus BY4742 [00-3] B 12 - - - - - - - - [00-3] C 1 - - - - - - - - [00-3] C 2 - - - - - - - - [00-3] C 3 YGR279C SCW4 - Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating conjugation hydrolase activity, acting on glycosyl bonds cell wall, extracellular region BY4742 [00-3] C 4 - - - - - - - - [00-3] C 5 YGR281W YOR1 YRS1 Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) response to chemical stimulus ATPase activity, transmembrane transporter activity membrane, membrane fraction, plasma membrane BY4742 [00-3] C 6 YGR282C BGL2 - Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds cell wall BY4742 [00-3] C 7 YGR283C - - Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus biological_process methyltransferase activity nucleus, nucleolus, ribosome, cytoplasm BY4742 [00-3] C 8 YGR284C ERV29 - Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm BY4742 [00-3] C 9 YGR285C ZUO1 zuotin Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p regulation of translation, rRNA processing, translational elongation, ribosomal subunit export from nucleus, protein folding, nuclear transport unfolded protein binding nucleus, ribosome, mitochondrion, nucleolus, cytoplasm BY4742 [00-3] C 10 YGR286C BIO2 - Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant cofactor metabolic process, vitamin metabolic process other mitochondrion, cytoplasm BY4742 [00-3] C 11 YGR287C IMA1 - Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization; has specificity for isomaltose, palatinose, and methyl-alpha-glucoside; member of the IMA isomaltase family carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, acting on glycosyl bonds ribosome, mitochondrion, cytoplasm BY4742 [00-3] C 12 YGR288W MAL13 MALR MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C carbohydrate metabolic process nucleic acid binding transcription factor activity nucleus BY4742 [00-3] D 1 - - - - - - - - [00-3] D 2 YGR290W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) biological_process molecular_function membrane BY4742 [00-3] D 3 - - - - - - - - [00-3] D 4 - - - - - - - - [00-3] D 5 - - - - - - - - [00-3] D 6 - - - - - - - - [00-3] D 7 YHR021W-A ECM12 - Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity cell wall organization or biogenesis molecular_function cellular_component BY4742 [00-3] D 8 YHR039C-B - - - - - - BY4742 [00-3] D 9 YHR079C-B - - - - - - BY4742 [00-3] D 10 YIL009C-A EST3 - Component of the telomerase holoenzyme, involved in telomere replication telomere organization, DNA replication GTPase activity, DNA binding, ATPase activity, helicase activity nucleus, chromosome BY4742 [00-3] D 11 YIR017C MET28 - Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, transcription factor binding nucleus BY4742 [00-3] D 12 YIR018W YAP5 - Basic leucine zipper (bZIP) iron-sensing transcription factor transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity nucleus, chromosome BY4742 [00-3] E 1 YIR019C MUC1 STA4|FLO11 GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain invasive growth in response to glucose limitation, pseudohyphal growth molecular_function cellular bud, membrane, extracellular region, site of polarized growth, plasma membrane BY4742 [00-3] E 2 YIR020C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-3] E 3 YIR020W-B - - - - - - BY4742 [00-3] E 4 YIR021W MRS1 PET157 Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA RNA splicing RNA binding mitochondrion, cytoplasm BY4742 [00-3] E 5 - - - - - - - - [00-3] E 6 - - - - - - - - [00-3] E 7 YIR024C - GIF1 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-3] E 8 YIR025W MND2 - Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, protein modification by small protein conjugation or removal, DNA recombination, regulation of cell cycle molecular_function nucleus BY4742 [00-3] E 9 YIR026C YVH1 - Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases meiotic cell cycle, sporulation, protein dephosphorylation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly, cell wall organization or biogenesis, signaling phosphatase activity nucleus, cytoplasm BY4742 [00-3] E 10 YIR027C DAL1 - Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component BY4742 [00-3] E 11 YIR028W DAL4 - Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other transmembrane transporter activity membrane BY4742 [00-3] E 12 YIR029W DAL2 ALC1 Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component BY4742 [00-3] F 1 - - - - - - - - [00-3] F 2 YIR031C DAL7 MSL2|MLS2 Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other transferase activity, transferring acyl groups cellular_component BY4742 F 3 - - - - - - - - [00-3] F 4 YIR033W MGA2 - ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting response to chemical stimulus, lipid metabolic process, transcription from RNA polymerase II promoter molecular_function nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-3] F 5 YIR034C LYS1 - Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity cellular amino acid metabolic process RNA binding, mRNA binding, oxidoreductase activity cytoplasm BY4742 [00-3] F 6 YIR035C - - Putative cytoplasmic protein of unknown function biological_process molecular_function membrane, cytoplasm BY4742 [00-3] F 7 YIR036C IRC24 - Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci biological_process molecular_function membrane, cytoplasm BY4742 [00-3] F 8 YIR037W HYR1 ORP1|GPX3 Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor response to oxidative stress, response to chemical stimulus oxidoreductase activity other BY4742 [00-3] F 9 YIR038C GTT1 - ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p cellular amino acid metabolic process oxidoreductase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups membrane fraction, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [00-3] F 10 YIR039C YPS6 - Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance cell wall organization or biogenesis peptidase activity cell wall BY4742 [00-3] F 11 YIR042C - - Putative protein of unknown function; YIR042C is a non-essential gene biological_process molecular_function cellular_component BY4742 F 12 - - - - - - - - G 1 - - - - - - - - [00-3] G 2 YKL033W-A - - Putative protein of unknown function; similar to uncharacterized proteins from other fungi biological_process molecular_function cellular_component BY4742 G 3 - - - - - - - - [00-3] G 4 YKL162C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-3] G 5 YKR035W-A DID2 VPL30|VPS46|CHM1|FTI1 Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors protein targeting, endosome transport molecular_function cytoplasm BY4742 [00-3] G 6 YKR066C CCP1 - Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-3] G 7 YKR067W GPT2 GAT1 Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis lipid metabolic process transferase activity, transferring acyl groups membrane, membrane fraction, endoplasmic reticulum, cytoplasm BY4742 G 8 - - - - - - - - [00-3] G 9 YKR069W MET1 MET20 S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis cofactor metabolic process, cellular amino acid metabolic process methyltransferase activity other BY4742 [00-3] G 10 YKR070W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 G 11 - - - - - - - - [00-3] G 12 YKR072C SIS2 HAL3 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis mitotic cell cycle, cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to osmotic stress, regulation of cell cycle enzyme regulator activity, lyase activity nucleus, cytoplasm BY4742 [00-3] H 1 YKR073C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-3] H 2 YKR074W AIM29 - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cytoplasm BY4742 [00-3] H 3 YKR075C - - Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm BY4742 [00-3] H 4 YKR076W ECM4 GTO2 Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm cell wall organization or biogenesis, cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm BY4742 [00-3] H 5 YKR077W MSA2 - Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation mitotic cell cycle, transcription from RNA polymerase II promoter molecular_function nucleus, cytoplasm BY4742 [00-3] H 6 YKR078W - - Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) biological_process lipid binding cytoplasm BY4742 H 7 - - - - - - - - [00-3] H 8 YKR080W MTD1 - NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity cytoplasm BY4742 H 9 - - - - - - - - [00-3] H 10 YKR082W NUP133 RAT3 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; required also for transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay response to heat, protein targeting, nucleobase-containing compound transport, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane BY4742 H 11 - - - - - - - - [00-3] H 12 YKR084C HBS1 - GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay RNA catabolic process GTPase activity cytoplasm BY4742 [00-4] A 1 - - - - - - - - [00-4] A 2 - - - - - - - - [00-4] A 3 YKR087C OMA1 - Metalloendopeptidase of the mitochondrial inner membrane, involved in turnover of membrane-embedded proteins; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes proteolysis involved in cellular protein catabolic process peptidase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-4] A 4 YKR088C TVP38 - Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern mitotic cell cycle, cytoskeleton organization molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-4] A 5 YKR089C TGL4 STC1 Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p lipid metabolic process, cell budding transferase activity, transferring acyl groups cytoplasm BY4742 [00-4] A 6 YKR090W PXL1 - LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin signaling enzyme regulator activity cellular bud, site of polarized growth, cytoplasm BY4742 [00-4] A 7 YKR091W SRL3 - Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate other molecular_function cytoplasm BY4742 [00-4] A 8 YKR092C SRP40 - Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 nuclear transport molecular_function nucleus, nucleolus BY4742 [00-4] A 9 YKR093W PTR2 - Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p other other membrane, membrane fraction, plasma membrane BY4742 [00-4] A 10 - - - - - - - - [00-4] A 11 - - - - - - - - [00-4] A 12 - - - - - - - - [00-4] B 1 YKR097W PCK1 PPC1|JPM2 Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol carbohydrate metabolic process lyase activity cytoplasm BY4742 [00-4] B 2 YKR098C UBP11 - Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins biological_process peptidase activity cellular_component BY4742 [00-4] B 3 YKR099W BAS1 - Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-4] B 4 YKR100C SKG1 YKR099C-A Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell cell wall organization or biogenesis molecular_function cellular bud, site of polarized growth BY4742 [00-4] B 5 YKR101W SIR1 - Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin chromatin organization DNA binding nucleus BY4742 [00-4] B 6 - - - - - - - - [00-4] B 7 YKR103W NFT1 - Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. other ATPase activity, transmembrane transporter activity membrane BY4742 [00-4] B 8 YKR104W - NFT1 Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds other ATPase activity, transmembrane transporter activity membrane BY4742 [00-4] B 9 YKR105C VBA5 - Putative transporter of the Major Facilitator Superfamily (MFS); proposed role as a basic amino acid permease based on phylogeny biological_process molecular_function membrane BY4742 [00-4] B 10 YLL018C-A COX19 - Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrion organization, ion transport, protein complex biogenesis ion binding mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-4] B 11 YLR262C-A TMA7 RBF7 Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin cytoplasmic translation molecular_function nucleus, cytoplasm BY4742 [00-4] B 12 - - - - - - - - [00-4] C 1 YLR422W - - Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein biological_process molecular_function cytoplasm BY4742 [00-4] C 2 YLR423C ATG17 APG17 Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells vacuole organization, protein phosphorylation, organelle assembly, peroxisome organization, regulation of protein modification process, membrane invagination, response to starvation enzyme regulator activity, structural molecule activity cytoplasm BY4742 [00-4] C 3 - - - - - - - - [00-4] C 4 YLR425W TUS1 SOP10 Guanine nucleotide exchange factor (GEF) that functions to modulate Rho1p activity as part of the cell integrity signaling pathway; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate cell wall organization or biogenesis, signaling enzyme regulator activity cellular_component BY4742 [00-4] C 5 YLR426W TDA5 - Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele response to chemical stimulus molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-4] C 6 YLR427W MAG2 - Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 biological_process molecular_function cytoplasm BY4742 [00-4] C 7 YLR428C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 biological_process molecular_function cellular_component BY4742 [00-4] C 8 YLR429W CRN1 - Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly cytoskeleton organization, regulation of organelle organization, protein complex biogenesis protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm BY4742 [00-4] C 9 - - - - - - - - [00-4] C 10 YLR431C ATG23 CVT23 Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p protein targeting, response to starvation molecular_function membrane fraction, cytoplasm BY4742 [00-4] C 11 YLR432W IMD3 - Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed nucleobase-containing small molecule metabolic process oxidoreductase activity membrane fraction, cytoplasm BY4742 [00-4] C 12 YLR433C CNA1 CMP1 Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 cellular ion homeostasis, conjugation, cell wall organization or biogenesis, signaling, response to chemical stimulus phosphatase activity cytoplasm BY4742 [00-4] D 1 YLR434C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W biological_process molecular_function cellular_component BY4742 [00-4] D 2 YLR435W TSR2 - Protein with a potential role in pre-rRNA processing rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, cytoplasm BY4742 [00-4] D 3 YLR436C ECM30 - Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm cell wall organization or biogenesis molecular_function cytoplasm BY4742 [00-4] D 4 YLR437C DIF1 - Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein biological_process molecular_function cytoplasm BY4742 [00-4] D 5 - - - - - - - - [00-4] D 6 YLR438W CAR2 cargB L-ornithine transaminase (OTAse), catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive cellular amino acid metabolic process other nucleus, cytoplasm BY4742 [00-4] D 7 - - - - - - - - [00-4] D 8 - - - - - - - - [00-4] D 9 YLR441C RPS1A rp10A|S1A|RP10A Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-4] D 10 - - - - - - - - [00-4] D 11 YLR443W ECM7 ZRG15 Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency ion transport, cell wall organization or biogenesis molecular_function membrane BY4742 [00-4] D 12 YLR444C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-4] E 1 YLR445W - - Putative protein of unknown function; transcription is regulated by Ume6p and induced in response to alpha factor biological_process molecular_function cellular_component BY4742 [00-4] E 2 YLR446W - - Putative protein of unknown function with similarity to hexokinases; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-4] E 3 YLR447C VMA6 - Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found in the endomembrane system; stabilizes VO subunits; required for V1 domain assembly on the vacuolar membrane cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-4] E 4 YLR448W RPL6B rp18|YL16|L6B|L17B Protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Ap and to rat L6 ribosomal protein; binds to 5.8S rRNA cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-4] E 5 YLR449W FPR4 - Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones peptidyl-amino acid modification, protein alkylation, chromatin organization, regulation of organelle organization, histone modification, regulation of protein modification process isomerase activity nucleus, chromosome BY4742 [00-4] E 6 YLR450W HMG2 - One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks lipid metabolic process oxidoreductase activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-4] E 7 YLR452C SST2 - GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family conjugation, signaling, response to chemical stimulus enzyme regulator activity membrane, plasma membrane BY4742 [00-4] E 8 YLR453C RIF2 - Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation telomere organization, DNA replication DNA binding nucleus, chromosome BY4742 [00-4] E 9 YLR454W FMP27 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-4] E 10 - - - - - - - - [00-4] E 11 YLR456W - - Putative protein of unknown function; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component BY4742 [00-4] E 12 - - - - - - - - [00-4] F 1 - - - - - - - - [00-4] F 2 - - - - - - - - [00-4] F 3 YLR460C - - Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine biological_process molecular_function cellular_component BY4742 [00-4] F 4 YLR461W PAU4 - Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component BY4742 [00-4] F 5 YML009C MRPL39 YmL39 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-4] F 6 YML010C-B - - - - - - BY4742 [00-4] F 7 YML021C UNG1 - Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus response to DNA damage stimulus, DNA repair hydrolase activity, acting on glycosyl bonds nucleus, mitochondrion, cytoplasm BY4742 [00-4] F 8 YML081C-A ATP18 - Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-4] F 9 YMR060C SAM37 TOM37|PET3027|MAS37 Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability protein targeting, mitochondrion organization, protein complex biogenesis, lipid transport molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-4] F 10 YMR158C-B - - - - - - BY4742 [00-4] F 11 YMR169C ALD3 - Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [00-4] F 12 YMR174C PAI3 IA3 Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact other enzyme regulator activity cytoplasm BY4742 [00-4] G 1 YMR175W SIP18 - Phospholipid-binding hydrophilin with a role in dessication resistance; expression is induced by osmotic stress response to chemical stimulus lipid binding cytoplasm BY4742 [00-4] G 2 YMR194C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-4] G 3 YMR326C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 biological_process molecular_function cellular_component BY4742 [00-4] G 4 YNR032C-A HUB1 - Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear RNA splicing, cell morphogenesis, conjugation, mRNA processing, response to chemical stimulus other other BY4742 [00-4] G 5 YNR050C LYS9 LYS13 Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [00-4] G 6 YNR051C BRE5 - Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Golgi vesicle transport, protein modification by small protein conjugation or removal, regulation of transport molecular_function cytoplasm BY4742 [00-4] G 7 - - - - - - - - [00-4] G 8 - - - - - - - - [00-4] G 9 - - - - - - - - [00-4] G 10 - - - - - - - - [00-4] G 11 YNR056C BIO5 - Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis cofactor metabolic process, vitamin metabolic process transmembrane transporter activity membrane, plasma membrane BY4742 [00-4] G 12 YNR057C BIO4 - Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels cofactor metabolic process, vitamin metabolic process ligase activity cytoplasm BY4742 [00-4] H 1 YNR058W BIO3 - 7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis cofactor metabolic process, vitamin metabolic process other cytoplasm BY4742 [00-4] H 2 YNR059W MNT4 - Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cellular_component BY4742 [00-4] H 3 YNR060W FRE4 - Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels cellular ion homeostasis, ion transport oxidoreductase activity membrane, plasma membrane BY4742 [00-4] H 4 YNR061C - - Putative protein of unknown function biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-4] H 5 YNR062C - - Putative membrane protein of unknown function biological_process molecular_function membrane BY4742 [00-4] H 6 YNR063W - - Putative zinc-cluster protein of unknown function biological_process DNA binding cellular_component BY4742 [00-4] H 7 YNR064C - - Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides response to chemical stimulus other other BY4742 [00-4] H 8 YNR065C - YSN1 Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene biological_process molecular_function membrane BY4742 [00-4] H 9 YNR066C - - Putative membrane-localized protein of unknown function biological_process molecular_function membrane, membrane fraction BY4742 [00-4] H 10 YNR067C DSE4 ENG1 Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother cytokinesis hydrolase activity, acting on glycosyl bonds cell wall, extracellular region BY4742 [00-4] H 11 YNR068C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-4] H 12 - - - - - - - - [00-5] A 1 - - - - - - - - [00-5] A 2 - - - - - - - - [00-5] A 3 YER101C AST2 - Protein that may have a role in targeting of plasma membrane [H+]ATPase (Pma1p) to the plasma membrane, as suggested by analysis of genetic interactions biological_process molecular_function cytoplasm BY4742 [00-5] A 4 - - - - - - - - [00-5] A 5 YER103W SSA4 YG107 Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation protein targeting, protein folding, transmembrane transport unfolded protein binding, ATPase activity nucleus, cytoplasm BY4742 [00-5] A 6 - - - - - - - - [00-5] A 7 - - - - - - - - [00-5] A 8 YER106W MAM1 - Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle meiotic cell cycle, chromosome segregation molecular_function nucleus, chromosome BY4742 [00-5] A 9 - - - - - - - - [00-5] A 10 YER108C - - - - - - BY4742 [00-5] A 11 YER109C FLO8 STA10|YER108C|PHD5 Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene carbohydrate metabolic process, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-5] A 12 YER110C KAP123 YRB4 Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 protein targeting, nuclear transport protein transporter activity nucleus, membrane, endomembrane system, cytoplasm BY4742 [00-5] B 1 YER111C SWI4 ART1 DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair other nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome BY4742 [00-5] B 2 - - - - - - - - [00-5] B 3 YER113C TMN3 - Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation cellular ion homeostasis, invasive growth in response to glucose limitation, pseudohyphal growth molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [00-5] B 4 YER114C BOI2 BEB1 Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain cell budding, cytokinesis, signaling lipid binding cellular bud, site of polarized growth BY4742 [00-5] B 5 YER115C SPR6 - Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation sporulation molecular_function cellular_component BY4742 [00-5] B 6 YER116C SLX8 - Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain response to DNA damage stimulus, telomere organization, protein modification by small protein conjugation or removal ligase activity nucleus BY4742 [00-5] B 7 YER117W RPL23B YL32|L23B|L17aB Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E. coli L14 and rat L23 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-5] B 8 YER118C SHO1 SSU81 Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p response to heat, signaling, response to osmotic stress structural molecule activity, signal transducer activity cellular bud, membrane, site of polarized growth, plasma membrane BY4742 [00-5] B 9 YER119C AVT6 - Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-5] B 10 YER119C-A - - Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 biological_process molecular_function cellular_component BY4742 [00-5] B 11 YER120W SCS2 - Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also involved in telomeric silencing, disruption causes inositol auxotrophy above 34 degrees C, VAP homolog protein targeting, transmembrane transport, regulation of transport, lipid transport, lipid metabolic process, organelle inheritance, nuclear transport lipid binding nucleus, endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm BY4742 [00-5] B 12 YER121W - - Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy biological_process molecular_function cellular_component BY4742 [00-5] C 1 YER122C GLO3 - ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p vesicle organization, Golgi vesicle transport enzyme regulator activity endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm BY4742 [00-5] C 2 YER123W YCK3 CKI3 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway protein phosphorylation kinase activity nucleus, membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-5] C 3 YER124C DSE1 - Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall invasive growth in response to glucose limitation, cytokinesis, response to chemical stimulus, conjugation, signaling molecular_function cellular bud, site of polarized growth BY4742 [00-5] C 4 - - - - - - - - [00-5] C 5 - - - - - - - - [00-5] C 6 - - - - - - - - [00-5] C 7 YER128W VFA1 - Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology other molecular_function cytoplasm BY4742 [00-5] C 8 YER129W SAK1 PAK1 Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome carbohydrate metabolic process, protein phosphorylation, DNA replication kinase activity cytoplasm BY4742 [00-5] C 9 YER130C - - Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; the C. Albicans homolog (MNL1) plays a role in adaptation to stress biological_process DNA binding cellular_component BY4742 [00-5] C 10 YER131W RPS26B S26B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similarity to rat S26 ribosomal protein cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-5] C 11 YER132C PMD1 - Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions sporulation molecular_function cytoplasm BY4742 [00-5] C 12 - - - - - - - - [00-5] D 1 YER134C - MDP-1 Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene biological_process phosphatase activity nucleus, cytoplasm BY4742 [00-5] D 2 YER135C - - Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-5] D 3 - - - - - - - - [00-5] D 4 YER137C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-5] D 5 YER139C RTR1 - CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm protein dephosphorylation, transcription from RNA polymerase II promoter phosphatase activity nucleus, cytoplasm BY4742 [00-5] D 6 YER140W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; genetic interactions suggest a role in folding of ER membrane proteins protein folding molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-5] D 7 YER141W COX15 - Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase cofactor metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-5] D 8 YER142C MAG1 MMS5 3-methyl-adenine DNA glycosylase involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired response to DNA damage stimulus, DNA repair hydrolase activity, acting on glycosyl bonds nucleus BY4742 [00-5] D 9 YER143W DDI1 VSM1 DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains proteolysis involved in cellular protein catabolic process peptidase activity, small conjugating protein binding membrane, plasma membrane BY4742 [00-5] D 10 YER145C FTR1 - High affinity iron permease involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-5] D 11 - - - - - - - - [00-5] D 12 - - - - - - - - [00-5] E 1 - - - - - - - - [00-5] E 2 YER149C PEA2 PPF2|DFG9 Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth cell morphogenesis, cell budding, cytoskeleton organization, cytokinesis, response to chemical stimulus, conjugation, pseudohyphal growth, signaling cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [00-5] E 3 YER150W SPI1 - GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p response to chemical stimulus molecular_function cell wall BY4742 [00-5] E 4 YER151C UBP3 BLM3 Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity regulation of transport, Golgi vesicle transport, protein modification by small protein conjugation or removal, response to osmotic stress peptidase activity, RNA binding, mRNA binding cytoplasm BY4742 [00-5] E 5 YER152C - - Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene biological_process other nucleus, cytoplasm BY4742 [00-5] E 6 YER153C PET122 - Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis RNA binding, translation factor activity, nucleic acid binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-5] E 7 YER154W OXA1 PET1402 Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals mitochondrion organization, protein complex biogenesis protein transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-5] E 8 YER155C BEM2 TSL1|SUP9|IPL2 Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence cytoskeleton organization, cell wall organization or biogenesis, signaling enzyme regulator activity cell cortex, mitochondrion, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [00-5] E 9 YER156C - - Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 biological_process molecular_function nucleus, cytoplasm BY4742 [00-5] E 10 - - - - - - - - [00-5] E 11 YER158C - - Protein of unknown function, has similarity to Afr1p; potentially phosphorylated by Cdc28p biological_process molecular_function cellular_component BY4742 [00-5] E 12 - - - - - - - - [00-5] F 1 YER161C SPT2 EXA1|SIN1 Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter DNA binding nucleus BY4742 [00-5] F 2 YER162C RAD4 - Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins response to DNA damage stimulus, DNA repair, proteolysis involved in cellular protein catabolic process DNA binding nucleus, cytoplasm BY4742 [00-5] F 3 YER163C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle; weak similarity to bacterial cation transport protein biological_process molecular_function nucleus, cytoplasm BY4742 [00-5] F 4 YER164W CHD1 - Chromatin remodeler that regulates various aspects of transcription; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes transcription from RNA polymerase I promoter, peptidyl-amino acid modification, protein acylation, chromatin organization, DNA replication, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, regulation of organelle organization, histone modification, protein complex biogenesis, regulation of protein modification process, transcription initiation, DNA-dependent, transcription termination, DNA-dependent, regulation of DNA metabolic process histone binding, DNA binding, ATPase activity nucleus, chromosome, mitochondrion, nucleolus, cytoplasm BY4742 [00-5] F 5 - - - - - - - - [00-5] F 6 YER166W DNF1 - Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase lipid transport, membrane invagination, endocytosis transmembrane transporter activity, ATPase activity mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [00-5] F 7 YER167W BCK2 CTR7 Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations mitotic cell cycle, regulation of cell cycle molecular_function nucleus, cytoplasm BY4742 [00-5] F 8 - - - - - - - - [00-5] F 9 YER169W RPH1 KDM4 JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway chromatin organization, transcription from RNA polymerase II promoter, histone modification nucleic acid binding transcription factor activity, DNA binding, oxidoreductase activity nucleus BY4742 [00-5] F 10 YER170W ADK2 PAK3|AKY3 Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background nucleobase-containing small molecule metabolic process kinase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-5] F 11 - - - - - - - - [00-5] F 12 - - - - - - - - [00-5] G 1 YER173W RAD24 RS1 Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination, regulation of cell cycle DNA binding, ATPase activity nucleus, chromosome BY4742 [00-5] G 2 YER174C GRX4 - Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance cellular ion homeostasis, cytoskeleton organization, response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus BY4742 [00-5] G 3 YER175C TMT1 TAM1 Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle biological_process methyltransferase activity cytoplasm BY4742 [00-5] G 4 YER176W ECM32 MTT1|HEL1 DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes regulation of translation helicase activity other BY4742 [00-5] G 5 YER177W BMH1 APR6 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling mitotic cell cycle, carbohydrate metabolic process, sporulation, generation of precursor metabolites and energy, response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, pseudohyphal growth, cell wall organization or biogenesis, signaling, regulation of cell cycle DNA binding nucleus, membrane fraction BY4742 [00-5] G 6 YER178W PDA1 - E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose cofactor metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-5] G 7 YER179W DMC1 ISC2 Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein meiotic cell cycle, DNA recombination DNA binding nucleus, chromosome BY4742 [00-5] G 8 YER180C ISC10 - Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells sporulation molecular_function cellular_component BY4742 [00-5] G 9 YER181C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-5] G 10 YER182W FMP10 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-5] G 11 YER183C FAU1 - 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis cofactor metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-5] G 12 YER184C - - Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source biological_process DNA binding cellular_component BY4742 [00-5] H 1 YER185W PUG1 - Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins other molecular_function membrane, plasma membrane BY4742 [00-5] H 2 YER186C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-5] H 3 YER187W - - Putative protein of unknown function; induced in respiratory-deficient cells biological_process molecular_function cellular_component BY4742 [00-5] H 4 - - - - - - - - [00-5] H 5 YMR052C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [00-5] H 6 YMR052W FAR3 - Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum conjugation, signaling, response to chemical stimulus molecular_function endoplasmic reticulum, cytoplasm BY4742 [00-5] H 7 YMR053C STB2 - Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p biological_process molecular_function nucleus, chromosome BY4742 [00-5] H 8 YMR054W STV1 - Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm, Golgi apparatus BY4742 [00-5] H 9 YMR055C BUB2 PAC7 Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage mitotic cell cycle, regulation of cell cycle enzyme regulator activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-5] H 10 YMR056C AAC1 - Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-5] H 11 YMR057C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 biological_process molecular_function cellular_component BY4742 [00-5] H 12 YMR058W FET3 - Ferro-O2-oxidoreductase required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases transmembrane transport, ion transport, response to chemical stimulus, cellular ion homeostasis transmembrane transporter activity, oxidoreductase activity membrane, plasma membrane BY4742 [00-6] A 1 - - - - - - - - [00-6] A 2 - - - - - - - - [00-6] A 3 YMR062C ARG7 ECM40 Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine cell wall organization or biogenesis, cellular amino acid metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm BY4742 [00-6] A 4 YMR063W RIM9 - Protein of unknown function, involved in the proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans PalI; putative membrane protein sporulation molecular_function membrane BY4742 [00-6] A 5 YMR064W AEP1 NCA1 Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase cytoplasmic translation molecular_function mitochondrion, cytoplasm BY4742 [00-6] A 6 YMR065W KAR5 FIG3 Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone organelle fusion, conjugation, nucleus organization molecular_function endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-6] A 7 YMR066W SOV1 - Mitochondrial protein of unknown function biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-6] A 8 YMR067C UBX4 CUI1 UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome sporulation, proteolysis involved in cellular protein catabolic process other nucleus, membrane fraction, cytoplasm BY4742 [00-6] A 9 YMR068W AVO2 - Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth cytoskeleton organization, cell wall organization or biogenesis, signaling molecular_function cytoplasm BY4742 [00-6] A 10 YMR069W NAT4 NAA40 N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups nucleus, cytoplasm BY4742 [00-6] A 11 YMR070W MOT3 [MOT3]|[MOT3+]|ROX7 Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion lipid metabolic process, transcription from RNA polymerase II promoter, response to osmotic stress DNA binding, transcription factor binding nucleus BY4742 [00-6] A 12 YMR071C TVP18 - Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-6] B 1 YMR072W ABF2 - Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation organelle inheritance, mitochondrion organization DNA binding chromosome, mitochondrion, cytoplasm BY4742 [00-6] B 2 YMR073C IRC21 - Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study response to chemical stimulus molecular_function cytoplasm BY4742 [00-6] B 3 - - - - - - - - [00-6] B 4 YMR075C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W biological_process molecular_function cellular_component BY4742 [00-6] B 5 YMR075W RCO1 - Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p chromatin organization, DNA replication, histone modification, regulation of DNA metabolic process, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter molecular_function nucleus, chromosome BY4742 [00-6] B 6 - - - - - - - - [00-6] B 7 YMR077C VPS20 VPL10|VPT20|CHM6 Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes endosome transport, vesicle organization, proteolysis involved in cellular protein catabolic process other membrane, cytoplasm BY4742 [00-6] B 8 YMR078C CTF18 CHL12 Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint mitotic cell cycle, DNA repair, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, DNA recombination molecular_function nucleus, chromosome, mitochondrion, cytoplasm BY4742 [00-6] B 9 - - - - - - - - [00-6] B 10 YMR080C NAM7 SUP113|UPF1|MOF4|IFS2 ATP-dependent RNA helicase of the SFI superfamily involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance RNA catabolic process, regulation of translation, protein modification by small protein conjugation or removal helicase activity, ATPase activity cytoplasm BY4742 [00-6] B 11 YMR081C ISF1 MBR3 Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant generation of precursor metabolites and energy, cellular respiration molecular_function cellular_component BY4742 [00-6] B 12 YMR082C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-6] C 1 YMR083W ADH3 - Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-6] C 2 YMR084W - - Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains biological_process molecular_function cellular_component BY4742 [00-6] C 3 YMR085W - - Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains biological_process molecular_function cellular_component BY4742 [00-6] C 4 YMR086C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-6] C 5 YMR086W - - Component of the eisosome with unknown function; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cell periphery; expression is repressed by cAMP; similar to A. gossypii SEG gene which is important for stabilizing eisosomes biological_process molecular_function cell cortex, ribosome, cytoplasm BY4742 [00-6] C 6 YMR087W - - Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain RNA splicing, tRNA processing phosphatase activity cellular_component BY4742 [00-6] C 7 YMR088C VBA1 - Permease of basic amino acids in the vacuolar membrane amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-6] C 8 YMR089C YTA12 RCA1 Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes protein targeting, mitochondrion organization, transmembrane transport, protein maturation, protein complex biogenesis peptidase activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-6] C 9 YMR090W - - Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability biological_process molecular_function cytoplasm BY4742 [00-6] C 10 YMR091C NPL6 RSC7 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions; involved in nuclear protein import and maintenance of proper telomere length protein targeting, chromatin organization, response to DNA damage stimulus, transcription elongation, DNA-dependent, nuclear transport, transcription from RNA polymerase II promoter ATPase activity nucleus BY4742 [00-6] C 11 YMR092C AIP1 - Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats cytoskeleton organization, response to osmotic stress cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [00-6] C 12 - - - - - - - - [00-6] D 1 YNR070W PDR18 - Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies other ATPase activity, transmembrane transporter activity membrane, mitochondrion, cytoplasm BY4742 [00-6] D 2 YNR071C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-6] D 3 YNR072W HXT17 - Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose carbohydrate transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-6] D 4 YNR073C - - Putative mannitol dehydrogenase biological_process oxidoreductase activity cellular_component BY4742 [00-6] D 5 YNR074C AIF1 CPD1 Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [00-6] D 6 YNR075W COS10 - Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins membrane invagination, endocytosis molecular_function nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-6] D 7 YOL013W-A - YOL013W-B Putative protein of unknown function; identified by SAGE biological_process molecular_function membrane BY4742 [00-6] D 8 YOL086C ADH1 ADC1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process oxidoreductase activity membrane fraction, cytoplasm BY4742 - [00-6] D 9 YOL087C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid biological_process small conjugating protein binding cytoplasm BY4742 [00-6] D 10 YOL088C MPD2 - Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p protein folding isomerase activity, oxidoreductase activity endoplasmic reticulum, cytoplasm BY4742 [00-6] D 11 YOL089C HAL9 - Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression transcription from RNA polymerase II promoter, response to osmotic stress DNA binding nucleus, mitochondrion, cytoplasm BY4742 [00-6] D 12 YOL090W MSH2 PMS5 Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity; Msh2p-Msh6p binds to and hydrolyzes ATP meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity nucleus, chromosome BY4742 [00-6] E 1 YOL091W SPO21 MPC70 Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation meiotic cell cycle, sporulation, cell wall organization or biogenesis structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-6] E 2 YOL092W - - Putative protein of unknown function; predicted to contain six transmembrane domains and is 58% similar to the uncharacterized ORF YBR147W biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-6] E 3 YOL093W TRM10 - tRNA methyltransferase, methylates the N-1 position of guanosine in tRNAs tRNA processing, RNA modification methyltransferase activity nucleus, cytoplasm BY4742 [00-6] E 4 - - - - - - - - [00-6] E 5 YOL095C HMI1 - Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD mitochondrion organization helicase activity, ATPase activity mitochondrion, cytoplasm BY4742 [00-6] E 6 YOL096C COQ3 - O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein cofactor metabolic process methyltransferase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-6] E 7 - - - - - - - - [00-6] E 8 YOL098C - - Putative metalloprotease biological_process molecular_function cytoplasm BY4742 [00-6] E 9 YOL099C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes biological_process molecular_function ribosome, cytoplasm BY4742 [00-6] E 10 YOL100W PKH2 - Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p protein phosphorylation, RNA catabolic process, cell wall organization or biogenesis, mRNA processing, signaling kinase activity nucleus, cell cortex, cytoplasm BY4742 [00-6] E 11 YOL101C IZH4 - Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism lipid metabolic process, cellular ion homeostasis ion binding membrane BY4742 [00-6] E 12 - - - - - - - - [00-6] F 1 YOL103W ITR2 HRB612 Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively other transmembrane transporter activity membrane, plasma membrane BY4742 [00-6] F 2 YOL104C NDJ1 TAM1 Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement meiotic cell cycle, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle DNA binding nucleus, chromosome BY4742 [00-6] F 3 YOL105C WSC3 - Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis response to heat, cytoskeleton organization, cell wall organization or biogenesis, peroxisome organization, signaling signal transducer activity site of polarized growth, membrane fraction BY4742 [00-6] F 4 YOL106W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [00-6] F 5 YOL107W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein biological_process molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [00-6] F 6 YOL108C INO4 - Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-6] F 7 YOL109W ZEO1 - Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria cell wall organization or biogenesis molecular_function membrane fraction, mitochondrion, membrane, mitochondrial envelope, cytoplasm, plasma membrane BY4742 [00-6] F 8 YOL110W SHR5 ERF4 Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization protein targeting, protein acylation, protein lipidation transferase activity, transferring acyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-6] F 9 YOL111C MDY2 GET5|TMA24 Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes protein targeting, cell morphogenesis, response to chemical stimulus, conjugation molecular_function nucleus, ribosome, cytoplasm BY4742 [00-6] F 10 YOL112W MSB4 - GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization cytoskeleton organization enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoplasm BY4742 [00-6] F 11 YOL113W SKM1 - Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth protein phosphorylation, transmembrane transport, cytokinesis, lipid transport kinase activity nucleus, membrane, plasma membrane BY4742 [00-6] F 12 YOL114C - - Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-6] G 1 YOL115W PAP2 TRF4 Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p DNA repair, snoRNA processing, RNA catabolic process, response to DNA damage stimulus nucleotidyltransferase activity, lyase activity nucleus, nucleolus BY4742 [00-6] G 2 YOL116W MSN1 PHD2|MSS10|HRB382|FUP1 Transcriptional activator involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus response to heat, carbohydrate metabolic process, invasive growth in response to glucose limitation, response to chemical stimulus, pseudohyphal growth, transcription from RNA polymerase II promoter, response to osmotic stress DNA binding nucleus BY4742 [00-6] G 3 YOL117W RRI2 CSN10 Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response protein modification by small protein conjugation or removal, conjugation, signaling, response to chemical stimulus molecular_function nucleus BY4742 [00-6] G 4 YOL118C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-6] G 5 YOL119C MCH4 - Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport other other membrane, vacuole, cytoplasm BY4742 [00-6] G 6 - - - - - - - - [00-6] G 7 YOL121C RPS19A rp55a|YS16A|S19A|S16aA Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Bp cytoplasmic translation, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-6] G 8 YOL122C SMF1 SBS1 Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins cellular ion homeostasis, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-6] G 9 - - - - - - - - [00-6] G 10 YOL124C TRM11 - Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain tRNA processing, RNA modification RNA binding, methyltransferase activity cytoplasm BY4742 [00-6] G 11 - - - - - - - - [00-6] G 12 YOL126C MDH2 - Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 carbohydrate metabolic process, protein targeting, peroxisome organization oxidoreductase activity cytoplasm BY4742 [00-6] H 1 - - - - - - - - [00-6] H 2 YOL128C YGK3 - Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation protein phosphorylation kinase activity cellular_component BY4742 [00-6] H 3 YOL129W VPS68 - Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria protein targeting, endosome transport molecular_function membrane, vacuole, mitochondrion, cytoplasm BY4742 [00-6] H 4 - - - - - - - - [00-6] H 5 YOL131W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-6] H 6 YOL132W GAS4 - 1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall sporulation, cell wall organization or biogenesis other cell wall, membrane BY4742 [00-6] H 7 - - - - - - - - [00-6] H 8 - - - - - - - - [00-6] H 9 - - - - - - - - [00-6] H 10 YOL136C PFK27 PFK-2 6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A carbohydrate metabolic process, generation of precursor metabolites and energy kinase activity cytoplasm BY4742 [00-6] H 11 YOL137W BSC6 - Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [00-6] H 12 YOL138C RTC1 SEA2 Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif biological_process molecular_function membrane, vacuole, ribosome, cytoplasm BY4742 [00-8] A 1 YBR232C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] A 2 - - - - - - - - [00-8] A 3 YDR424C DYN2 SLC1 Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex protein complex biogenesis, nucleus organization molecular_function peroxisome, nucleus, cytoskeleton, endomembrane system, membrane, cytoplasm BY4742 [00-8] A 4 YEL011W GLC3 GHA1 Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups cytoplasm BY4742 [00-8] A 5 - - - - - - - - [00-8] A 6 YER064C VHR2 - Non-essential nuclear protein; null mutation has global effects on transcription other molecular_function nucleus BY4742 [00-8] A 7 YER077C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-8] A 8 YER078C ICP55 - Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family protein maturation peptidase activity nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-8] A 9 YER088C DOT6 PBF2 Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; similar to Tod6p; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation cell morphogenesis, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome, cytoplasm BY4742 [00-8] A 10 - - - - - - - - [00-8] A 11 YER090W TRP2 - Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-8] A 12 YER091C MET6 - Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs cellular amino acid metabolic process methyltransferase activity membrane fraction, cytoplasm BY4742 [00-8] B 1 YER092W IES5 - Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions biological_process molecular_function nucleus BY4742 [00-8] B 2 - - - - - - - - [00-8] B 3 YER093C-A AIM11 GEP8 Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron biological_process molecular_function cellular_component BY4742 [00-8] B 4 - - - - - - - - [00-8] B 5 YER095W RAD51 MUT5 Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein meiotic cell cycle, telomere organization, DNA recombination DNA binding, ATPase activity nucleus, chromosome BY4742 [00-8] B 6 YER096W SHC1 - Sporulation-specific activator of Chs3p (chitin synthase III), required for the synthesis of the chitosan layer of ascospores; has similarity to Skt5p, which activates Chs3p during vegetative growth; transcriptionally induced at alkaline pH carbohydrate metabolic process, sporulation enzyme regulator activity membrane BY4742 [00-8] B 7 YER097W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] B 8 YER098W UBP9 - Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions protein modification by small protein conjugation or removal peptidase activity cytoplasm BY4742 [00-8] B 9 YGR134W CAF130 - Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation transcription from RNA polymerase II promoter molecular_function nucleus BY4742 [00-8] B 10 - - - - - - - - [00-8] B 11 YGR210C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-8] B 12 YHL024W RIM4 - Putative RNA-binding protein required for the expression of early and middle sporulation genes meiotic cell cycle, DNA replication, sporulation, DNA recombination RNA binding cytoplasm BY4742 [00-8] C 1 YHL025W SNF6 - Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p response to DNA damage stimulus, DNA repair, chromatin organization molecular_function nucleus BY4742 [00-8] C 2 YHR016C YSC84 LSB4 Actin-binding protein involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p membrane invagination, cytoskeleton organization, endocytosis cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm BY4742 [00-8] C 3 YHR017W YSC83 - Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-8] C 4 YHR032W ERC1 - Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine cofactor metabolic process, transmembrane transport, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transmembrane transporter activity membrane BY4742 [00-8] C 5 YHR045W - - Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum biological_process molecular_function endoplasmic reticulum, cytoplasm BY4742 [00-8] C 6 YHR064C SSZ1 PDR13 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP cytoplasmic translation, regulation of translation, rRNA processing, translational elongation unfolded protein binding cytoplasm BY4742 [00-8] C 7 YHR140W - - Putative integral membrane protein of unknown function biological_process molecular_function membrane BY4742 [00-8] C 8 YHR162W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-8] C 9 YHR168W MTG2 - Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly cytoplasmic translation GTPase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-8] C 10 - - - - - - - - [00-8] C 11 - - - - - - - - [00-8] C 12 YHR181W SVP26 ERV26 Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment carbohydrate metabolic process, protein glycosylation, Golgi vesicle transport, cell wall organization or biogenesis protein binding, bridging endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-8] D 1 YHR191C CTF8 - Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion mitotic cell cycle, organelle fission, chromosome segregation molecular_function chromosome BY4742 [00-8] D 2 YHR193C EGD2 - Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes protein folding unfolded protein binding, lipid binding cytoplasm BY4742 [00-8] D 3 YLL030C RRT7 - Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] D 4 YLL044W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant biological_process molecular_function cellular_component BY4742 [00-8] D 5 YLL048C YBT1 BAT1 Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters other ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-8] D 6 YLL049W LDB18 - Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 mitotic cell cycle, cytoskeleton organization molecular_function cell cortex, cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-8] D 7 YLL059C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] D 8 YLR030W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-8] D 9 YLR031W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-8] D 10 YLR032W RAD5 SNM2|REV2 DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p DNA repair, protein complex biogenesis, response to DNA damage stimulus, protein modification by small protein conjugation or removal DNA binding, ATPase activity, helicase activity nucleus, chromosome BY4742 [00-8] D 11 - - - - - - - - [00-8] D 12 YLR034C SMF3 - Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins cellular ion homeostasis, ion transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-8] E 1 YLR035C MLH2 - Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination molecular_function nucleus BY4742 [00-8] E 2 YLR036C - - Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein biological_process molecular_function membrane BY4742 [00-8] E 3 YLR037C PAU23 DAN2 Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function cell wall BY4742 [00-8] E 4 YLR038C COX12 - Subunit VIb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated mitochondrion organization, protein complex biogenesis transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-8] E 5 YLR039C RIC1 - Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes endosome transport enzyme regulator activity nucleus, membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-8] E 6 YLR040C - - Putative protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; YLR040C is not essential biological_process molecular_function cell wall BY4742 [00-8] E 7 YLR041W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C biological_process molecular_function cellular_component BY4742 [00-8] E 8 YLR050C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene biological_process molecular_function membrane, endoplasmic reticulum, cytoplasm BY4742 [00-8] E 9 YLR052W IES3 - Subunit of the INO80 chromatin remodeling complex telomere organization, chromatin organization molecular_function nucleus, chromosome BY4742 [00-8] E 10 - - - - - - - - [00-8] E 11 YMR142C RPL13B L13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential for viability; has similarity to rat L13 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-8] E 12 YMR158W MRPS8 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-8] F 1 YMR171C EAR1 - Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process molecular_function cytoplasm BY4742 [00-8] F 2 YMR181C - - Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); YMR181C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-8] F 3 YMR209C - - Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene biological_process methyltransferase activity cellular_component BY4742 [00-8] F 4 YMR271C URA10 - Minor orotate phosphoribosyltransferase (OPRTase) isozyme that catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; major OPRTase encoded by URA5 nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups cytoplasm BY4742 [00-8] F 5 YMR279C - - Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; paralog of the efflux pump ATR1 transmembrane transport, ion transport transmembrane transporter activity membrane BY4742 [00-8] F 6 YMR306W FKS3 - Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies sporulation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups membrane, mitochondrion, cytoplasm BY4742 [00-8] F 7 YMR311C GLC8 - Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2 carbohydrate metabolic process, generation of precursor metabolites and energy, chromosome segregation enzyme regulator activity nucleus, cytoplasm BY4742 [00-8] F 8 YMR312W ELP6 KTI4|TOT6|HAP3 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity tRNA processing, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, RNA modification molecular_function other BY4742 [00-8] F 9 YMR313C TGL3 - Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation lipid metabolic process, cell budding transferase activity, transferring acyl groups cytoplasm BY4742 [00-8] F 10 - - - - - - - - [00-8] F 11 YMR315W - - Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-8] F 12 YMR316C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene DIA1/YMR316W biological_process molecular_function cellular_component BY4742 [00-8] G 1 YMR316C-B - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] G 2 YMR316W DIA1 - Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern invasive growth in response to glucose limitation, pseudohyphal growth molecular_function cytoplasm BY4742 [00-8] G 3 YMR317W - - Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-8] G 4 YMR318C ADH6 ADHVI NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance other oxidoreductase activity other BY4742 [00-8] G 5 YMR319C FET4 - Low-affinity Fe(II) transporter of the plasma membrane transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-8] G 6 YMR320W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] G 7 - - - - - - - - [00-8] G 8 YNL250W RAD50 - Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining meiotic cell cycle, DNA repair, telomere organization, response to DNA damage stimulus, DNA recombination kinase activity, DNA binding, ATPase activity nucleus, mitochondrion, cytoplasm BY4742 [00-8] G 9 YNL252C MRPL17 YmL17|YmL30|MRPL30 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-8] G 10 - - - - - - - - [00-8] G 11 - - - - - - - - [00-8] G 12 YNL279W PRM1 - Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p membrane fusion, conjugation, response to chemical stimulus molecular_function membrane, site of polarized growth BY4742 [00-8] H 1 - - - - - - - - [00-8] H 2 YNL300W TOS6 - Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid biological_process molecular_function cell wall BY4742 [00-8] H 3 - - - - - - - - [00-8] H 4 YNL316C PHA2 - Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-8] H 5 YOL016C CMK2 - Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk1p and mammalian Cam Kinase II protein phosphorylation, signaling kinase activity cytoplasm BY4742 [00-8] H 6 - - - - - - - - [00-8] H 7 YOR096W RPS7A rp30|S7A|RPS30 Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-8] H 8 YOR306C MCH5 - Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport other transmembrane transporter activity membrane, plasma membrane BY4742 [00-8] H 9 YOR317W FAA1 - Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles cofactor metabolic process, lipid transport ligase activity membrane fraction, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-8] H 10 - - - - - - - - [00-8] H 11 YPL132W COX11 PSO7|LPI13 Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration ion binding ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-8] H 12 YPL134C ODC1 2-oxodicarboxylate transporter Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation other transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] A 1 - - - - - - - - [00-9] A 2 - - - - - - - - [00-9] A 3 YML070W DAK1 - Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation carbohydrate metabolic process kinase activity cytoplasm BY4742 [00-9] A 4 YML071C COG8 DOR1 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting molecular_function cytoplasm, Golgi apparatus BY4742 [00-9] A 5 - - - - - - - - [00-9] A 6 YML074C FPR3 NPI46 Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p meiotic cell cycle, regulation of cell cycle isomerase activity nucleus, nucleolus BY4742 [00-9] A 7 YML090W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source biological_process molecular_function membrane BY4742 [00-9] A 8 - - - - - - - - [00-9] A 9 - - - - - - - - [00-9] A 10 - - - - - - - - [00-9] A 11 YML094W GIM5 PFD5 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it protein complex biogenesis cytoskeletal protein binding cytoplasm BY4742 [00-9] A 12 YML095C RAD10 - Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination DNA binding, nuclease activity nucleus BY4742 [00-9] B 1 YML095C-A - - - - - - BY4742 [00-9] B 2 YML096W - - Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 biological_process molecular_function cytoplasm BY4742 [00-9] B 3 YML097C VPS9 VPT9|VPL31 A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin vacuole organization, protein targeting, organelle inheritance small conjugating protein binding, enzyme regulator activity cytoplasm BY4742 [00-9] B 4 - - - - - - - - [00-9] B 5 YML099C ARG81 ARGRII|ARGR2 Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p exocytosis, regulation of transport, cellular amino acid metabolic process protein binding transcription factor activity nucleus BY4742 [00-9] B 6 YML100W TSL1 - Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, similar to Tps3p and may share function; mutant has aneuploidy tolerance carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, phosphatase activity, enzyme regulator activity cytoplasm BY4742 [00-9] B 7 YML100W-A - - Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component BY4742 [00-9] B 8 YML101C CUE4 - Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination biological_process molecular_function endoplasmic reticulum, cytoplasm BY4742 [00-9] B 9 YML102C-A - - - - - - BY4742 [00-9] B 10 YML102W CAC2 - Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription chromatin organization histone binding nucleus, chromosome BY4742 [00-9] B 11 YML103C NUP188 - Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p response to heat, protein targeting, nucleobase-containing compound transport, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane BY4742 [00-9] B 12 YML104C MDM1 - Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) organelle inheritance, mitochondrion organization structural molecule activity, lipid binding membrane, cytoskeleton, cytoplasm BY4742 [00-9] C 1 - - - - - - - - [00-9] C 2 YML106W URA5 PYR5 Major orotate phosphoribosyltransferase (OPRTase) isozyme that catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; minor OPRTase encoded by URA10 nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm BY4742 [00-9] C 3 YML107C PML39 - Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes other molecular_function nucleus, membrane, endomembrane system, ribosome, cytoplasm BY4742 [00-9] C 4 YML108W - - Putative protein of unknown function whose structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [00-9] C 5 YML109W ZDS2 CES4 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; paralog of Zds1p mitotic cell cycle, regulation of cell cycle molecular_function cellular bud, nucleus, site of polarized growth, cytoplasm BY4742 [00-9] C 6 YML110C COQ5 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase|DBI56 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration methyltransferase activity mitochondrion, cytoplasm BY4742 [00-9] C 7 YML111W BUL2 - Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1 protein modification by small protein conjugation or removal ligase activity cytoplasm BY4742 [00-9] C 8 YML112W CTK3 - Gamma subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity transcription from RNA polymerase I promoter, regulation of translation, protein phosphorylation, transcription elongation, DNA-dependent, mRNA processing, translational elongation kinase activity nucleus, nucleolus BY4742 [00-9] C 9 YML113W DAT1 - DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability transcription from RNA polymerase II promoter DNA binding nucleus BY4742 [00-9] C 10 - - - - - - - - [00-9] C 11 - - - - - - - - [00-9] C 12 YML116W ATR1 SNQ1 Multidrug efflux pump of the major facilitator superfamily, required for resistance to aminotriazole and 4-nitroquinoline-N-oxide transmembrane transport, ion transport, response to chemical stimulus transmembrane transporter activity vacuole, membrane, cytoplasm, plasma membrane BY4742 [00-9] D 1 YML117W NAB6 - Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP biological_process molecular_function cytoplasm BY4742 [00-9] D 2 YML117W-A - - - - - - BY4742 [00-9] D 3 YML118W NGL3 - 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p RNA catabolic process nuclease activity cellular_component BY4742 [00-9] D 4 YML119W - - Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate biological_process molecular_function cellular_component BY4742 [00-9] D 5 YML120C NDI1 - NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-9] D 6 YML121W GTR1 - Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB transcription from RNA polymerase I promoter, ion transport, transcription from RNA polymerase III promoter other nucleus, membrane, vacuole, cytoplasm BY4742 [00-9] D 7 YML122C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-9] D 8 YML123C PHO84 phoT High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-9] D 9 YML124C TUB3 - Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p mitotic cell cycle, meiotic cell cycle, organelle fission, chromosome segregation, conjugation structural molecule activity nucleus, cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-9] D 10 - - - - - - - - [00-9] D 11 - - - - - - - - [00-9] D 12 - - - - - - - - [00-9] E 1 YML128C MSC1 - Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated meiotic cell cycle, DNA recombination molecular_function membrane fraction, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-9] E 2 YML129C COX14 - Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] E 3 - - - - - - - - [00-9] E 4 YML131W - - Putative protein of unknown function with similarity to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-9] E 5 YMR004W MVP1 - Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions protein targeting lipid binding nucleus, cytoplasm BY4742 [00-9] E 6 - - - - - - - - [00-9] E 7 YMR095C SNO1 - Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase vitamin metabolic process molecular_function cytoplasm BY4742 [00-9] E 8 YMR096W SNZ1 - Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins vitamin metabolic process other cellular_component BY4742 [00-9] E 9 YMR097C MTG1 - Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals mitochondrial translation, mitochondrion organization GTPase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] E 10 YMR098C ATP25 LRC4 Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] E 11 YMR099C - hexose-6-phosphate mutarotase|glucose-6-phosphate 1-epimerase Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS carbohydrate metabolic process isomerase activity nucleus, cytoplasm BY4742 [00-9] E 12 YMR100W MUB1 - MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene cell budding, protein modification by small protein conjugation or removal molecular_function cellular_component BY4742 [00-9] F 1 YMR101C SRT1 - Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase protein glycosylation, carbohydrate metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm BY4742 [00-9] F 2 YMR102C - - Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-9] F 3 YMR103C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-9] F 4 - - - - - - - - [00-9] F 5 YMR105C PGM2 GAL5 Phosphoglucomutase, catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase carbohydrate metabolic process, cellular ion homeostasis, generation of precursor metabolites and energy, oligosaccharide metabolic process isomerase activity cytoplasm BY4742 [00-9] F 6 YMR106C YKU80 HDF2 Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair DNA repair, telomere organization, chromatin organization, response to DNA damage stimulus, DNA recombination RNA binding, DNA binding nucleus, chromosome BY4742 [00-9] F 7 YMR107W SPG4 - Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component BY4742 [00-9] F 8 - - - - - - - - [00-9] F 9 YMR109W MYO5 - One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization cell budding, cytokinesis, membrane invagination, exocytosis, cell wall organization or biogenesis, response to osmotic stress, endocytosis other cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [00-9] F 10 YMR110C HFD1 - Putative fatty aldehyde dehydrogenase, located in the mitochondrial outer membrane and also in lipid particles; has similarity to human fatty aldehyde dehydrogenase (FALDH) which is implicated in Sjogren-Larsson syndrome other oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] F 11 YMR111C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene biological_process molecular_function nucleus BY4742 [00-9] F 12 - - - - - - - - [00-9] G 1 - - - - - - - - [00-9] G 2 YMR114C - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene biological_process molecular_function nucleus, ribosome, cytoplasm BY4742 [00-9] G 3 YMR115W MGR3 FMP24 Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] G 4 YMR116C ASC1 NAD1|CPC2 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; represses Gcn4p in the absence of amino acid starvation regulation of translation, invasive growth in response to glucose limitation, response to chemical stimulus, signaling enzyme regulator activity, signal transducer activity ribosome, cytoplasm BY4742 [00-9] G 5 - - - - - - - - [00-9] G 6 - - - - - - - - [00-9] G 7 - - - - - - - - [00-9] G 8 YMR119W-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-9] G 9 YMR120C ADE17 - Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade16p; ade16 ade17 mutants require adenine and histidine nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds membrane fraction, cytoplasm BY4742 [00-9] G 10 YMR121C RPL15B rp15R|YL10|L15B|L13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-9] G 11 YMR122C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-9] G 12 YMR123W PKR1 - V-ATPase assembly factor, functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0) protein complex biogenesis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-9] H 1 YMR124W - - Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization biological_process molecular_function cytoplasm BY4742 [00-9] H 2 YMR125W STO1 SUT1|CBP80|GCR3|CBC1 Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 RNA catabolic process, RNA splicing, mRNA processing, response to osmotic stress RNA binding, mRNA binding nucleus BY4742 [00-9] H 3 YMR126C DLT1 - Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) biological_process molecular_function cellular_component BY4742 [00-9] H 4 YMR127C SAS2 KAT8 Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups nucleus, chromosome BY4742 [00-9] H 5 - - - - - - - - [00-9] H 6 YMR129W POM152 - Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase; protein targeting, cytoskeleton organization, nuclear transport, nucleus organization other nucleus, endomembrane system, mitochondrion, membrane, cytoplasm BY4742 [00-9] H 7 YMR130W - - Putative protein of unknown function; YMR130W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-9] H 8 - - - - - - - - [00-9] H 9 YMR132C JLP2 - Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) biological_process molecular_function cytoplasm BY4742 [00-9] H 10 YMR133W REC114 - Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division meiotic cell cycle, DNA recombination molecular_function nucleus, chromosome BY4742 [00-9] H 11 - - - - - - - - [00-9] H 12 YMR135C GID8 DCR1 Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains LisH and CTLH domains, like Vid30p; dosage-dependent regulator of START mitotic cell cycle, carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process, regulation of cell cycle molecular_function nucleus, cytoplasm BY4742 [00-10] A 1 YEL012W UBC8 GID3 Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro carbohydrate metabolic process, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity cytoplasm BY4742 [00-10] A 2 - - - - - - - - [00-10] A 3 - - - - - - - - [00-10] A 4 - - - - - - - - [00-10] A 5 - - - - - - - - [00-10] A 6 - - - - - - - - [00-10] A 7 YER031C YPT31 YPT8 Rab family GTPase, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi Golgi vesicle transport, exocytosis, endosome transport GTPase activity mitochondrion, membrane, mitochondrial envelope, Golgi apparatus, cytoplasm BY4742 [00-10] A 8 YER046W SPO73 - Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis sporulation, cell wall organization or biogenesis molecular_function cytoplasm BY4742 [00-10] A 9 YER063W THO1 - Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation nucleobase-containing compound transport, nuclear transport RNA binding, DNA binding nucleus BY4742 [00-10] A 10 YER066W RRT13 - Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component BY4742 [00-10] A 11 - - - - - - - - [00-10] A 12 - - - - - - - - [00-10] B 1 YGR188C BUB1 - Protein kinase involved in the cell cycle checkpoint into anaphase; forms complex with Mad1p and Bub3p crucial to preventing cell cycle progression into anaphase in the presence of spindle damage; CDC28-mediated phosphorylation at Bub1p-T566 is important for its degradation in anaphase and adaptation of the checkpoint to prolonged mitotic arrest, but not to its kinase activity or chromosome segregation function; associates with centromere DNA via Skp1p mitotic cell cycle, protein phosphorylation, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle kinase activity nucleus, chromosome BY4742 [00-10] B 2 YGR201C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-10] B 3 YGR204W ADE3 - Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ligase activity, oxidoreductase activity nucleus, cytoplasm BY4742 [00-10] B 4 - - - - - - - - [00-10] B 5 YHL002W HSE1 - Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes protein targeting, endosome transport small conjugating protein binding cytoplasm BY4742 [00-10] B 6 - - - - - - - - [00-10] B 7 YHL011C PRS3 - 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes carbohydrate metabolic process, cell wall organization or biogenesis other cytoplasm BY4742 [00-10] B 8 - - - - - - - - [00-10] B 9 YHL039W EFM1 - Probable lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm peptidyl-amino acid modification, protein alkylation methyltransferase activity cytoplasm BY4742 [00-10] B 10 - - - - - - - - [00-10] B 11 - - - - - - - - [00-10] B 12 YHR003C - - Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-10] C 1 YHR004C NEM1 - Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard lipid metabolic process, sporulation, nucleus organization phosphatase activity membrane, endomembrane system, mitochondrion, cytoplasm BY4742 [00-10] C 2 YHR005C GPA1 SCG1|DAC1|CDC70 GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome protein targeting, regulation of transport, organelle fusion, response to chemical stimulus, conjugation, nucleobase-containing small molecule metabolic process, nuclear transport, signaling, nucleus organization GTPase activity membrane, cytoplasm, plasma membrane BY4742 [00-10] C 3 YHR006W STP2 - Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-10] C 4 - - - - - - - - [00-10] C 5 YHR008C SOD2 - Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-10] C 6 YHR009C TDA3 BTN3 Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 endosome transport molecular_function cytoplasm BY4742 [00-10] C 7 - - - - - - - - [00-10] C 8 YHR025W THR1 - Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway cellular amino acid metabolic process kinase activity cellular_component BY4742 [00-10] C 9 YHR026W VMA16 PPA1 Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-10] C 10 - - - - - - - - [00-10] C 11 YHR041C SRB2 MED20|HRS2 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, response to chemical stimulus protein binding transcription factor activity nucleus BY4742 [00-10] C 12 - - - - - - - - [00-10] D 1 YHR059W FYV4 - Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-10] D 2 - - - - - - - - [00-10] D 3 YHR067W HTD2 RMD12 Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology lipid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [00-10] D 4 - - - - - - - - [00-10] D 5 - - - - - - - - [00-10] D 6 - - - - - - - - [00-10] D 7 - - - - - - - - [00-10] D 8 - - - - - - - - [00-10] D 9 - - - - - - - - [00-10] D 10 - - - - - - - - [00-10] D 11 YHR127W - HSN1 Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis mitotic cell cycle, cytoskeleton organization molecular_function nucleus BY4742 [00-10] D 12 - - - - - - - - [00-10] E 1 YHR131C - - Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study biological_process molecular_function cytoplasm BY4742 [00-10] E 2 - - - - - - - - [00-10] E 3 - - - - - - - - [00-10] E 4 - - - - - - - - [00-10] E 5 - - - - - - - - [00-10] E 6 - - - - - - - - [00-10] E 7 - - - - - - - - [00-10] E 8 - - - - - - - - [00-10] E 9 YHR180W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-10] E 10 YHR185C PFS1 ADY1 Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation sporulation, cell wall organization or biogenesis molecular_function cellular_component BY4742 [00-10] E 11 - - - - - - - - [00-10] E 12 - - - - - - - - [00-10] F 1 YHR194W MDM31 - Mitochondrial inner membrane protein with similarity to Mdm32p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 mitochondrion organization, cellular ion homeostasis, organelle inheritance molecular_function nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-10] F 2 - - - - - - - - [00-10] F 3 - - - - - - - - [00-10] F 4 - - - - - - - - [00-10] F 5 YLL007C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene biological_process molecular_function cytoplasm BY4742 [00-10] F 6 - - - - - - - - [00-10] F 7 YMR154C RIM13 CPL1 Calpain-like cysteine protease involved in proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans palB protein maturation peptidase activity cellular_component BY4742 [00-10] F 8 - - - - - - - - [00-10] F 9 YNL274C GOR1 - Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies other oxidoreductase activity nucleus, mitochondrion, cytoplasm BY4742 [00-10] F 10 - - - - - - - - [00-10] F 11 - - - - - - - - [00-10] F 12 - - - - - - - - [00-10] G 1 - - - - - - - - [00-10] G 2 YOL141W PPM2 TYW4 AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p tRNA processing, nucleobase-containing small molecule metabolic process, RNA modification methyltransferase activity mitochondrion, cytoplasm BY4742 [00-10] G 3 - - - - - - - - [00-10] G 4 YOL143C RIB4 - Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin vitamin metabolic process isomerase activity nucleus, cytoplasm BY4742 [00-10] G 5 - - - - - - - - [00-10] G 6 - - - - - - - - [00-10] G 7 - - - - - - - - [00-10] G 8 - - - - - - - - [00-10] G 9 - - - - - - - - [00-10] G 10 - - - - - - - - [00-10] G 11 YOL150C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-10] G 12 - - - - - - - - [00-10] H 1 - - - - - - - - [00-10] H 2 - - - - - - - - [00-10] H 3 - - - - - - - - [00-10] H 4 YOL158C ENB1 ARN4 Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment cellular ion homeostasis, ion transport transmembrane transporter activity cellular bud, membrane, plasma membrane BY4742 [00-10] H 5 YOL159C - - Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA biological_process molecular_function other BY4742 [00-10] H 6 YOL160W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-10] H 7 YOL162W - - Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity membrane BY4742 [00-10] H 8 YOL163W - - Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity membrane BY4742 [00-10] H 9 - - - - - - - - [00-10] H 10 - - - - - - - - [00-10] H 11 - - - - - - - - [00-10] H 12 YOR008C-A - - Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres biological_process molecular_function cellular_component BY4742 [00-11] A 1 - - - - - - - - [00-11] A 2 - - - - - - - - [00-11] A 3 - - - - - - - - [00-11] A 4 - - - - - - - - [00-11] A 5 - - - - - - - - [00-11] A 6 - - - - - - - - [00-11] A 7 YBR191W RPL21A L21A|URP1 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-11] A 8 - - - - - - - - [00-11] A 9 YCL035C GRX1 - Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage response to oxidative stress, response to chemical stimulus transferase activity, transferring alkyl or aryl (other than methyl) groups, oxidoreductase activity nucleus, cytoplasm BY4742 [00-11] A 10 - - - - - - - - [00-11] A 11 - - - - - - - - [00-11] A 12 - - - - - - - - [00-11] B 1 - - - - - - - - [00-11] B 2 - - - - - - - - [00-11] B 3 - - - - - - - - [00-11] B 4 - - - - - - - - [00-11] B 5 - - - - - - - - [00-11] B 6 - - - - - - - - [00-11] B 7 YDR071C PAA1 - Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication chromatin organization transferase activity, transferring acyl groups cytoplasm BY4742 [00-11] B 8 YDR074W TPS2 PFK3|HOG2 Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway carbohydrate metabolic process, oligosaccharide metabolic process phosphatase activity mitochondrion, cytoplasm BY4742 [00-11] B 9 - - - - - - - - [00-11] B 10 YER027C GAL83 SPM1 One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain protein phosphorylation, invasive growth in response to glucose limitation, protein complex biogenesis, signaling kinase activity nucleus, endomembrane system, cytoplasm BY4742 [00-11] B 11 YER037W PHM8 - Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p response to starvation phosphatase activity nucleus, cytoplasm BY4742 [00-11] B 12 YGR155W CYS4 VMA41|STR4|NHS5 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; mutations in human ortholog cause homocystinuria cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [00-11] C 1 - - - - - - - - [00-11] C 2 - - - - - - - - [00-11] C 3 - - - - - - - - [00-11] C 4 - - - - - - - - [00-11] C 5 - - - - - - - - [00-11] C 6 - - - - - - - - [00-11] C 7 - - - - - - - - [00-11] C 8 YLR192C HCR1 - Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis RNA binding, translation factor activity, nucleic acid binding cytoplasm BY4742 [00-11] C 9 - - - - - - - - [00-11] C 10 YLR237W THI7 THI10 Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia other transmembrane transporter activity membrane, plasma membrane BY4742 [00-11] C 11 YLR246W ERF2 - Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole protein targeting, protein acylation, protein lipidation transferase activity, transferring acyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-11] C 12 - - - - - - - - [00-11] D 1 YLR334C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here biological_process molecular_function membrane BY4742 [00-11] D 2 - - - - - - - - [00-11] D 3 YLR346C - - Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-11] D 4 YLR358C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSC2/YLR357W biological_process molecular_function cellular_component BY4742 [00-11] D 5 YLR361C DCR2 - Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START mitotic cell cycle, protein dephosphorylation, signaling, response to chemical stimulus, regulation of cell cycle phosphatase activity cellular_component BY4742 [00-11] D 6 YLR370C ARC18 - Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches organelle inheritance, mitochondrion organization, cytoskeleton organization molecular_function cytoskeleton, mitochondrion, cytoplasm BY4742 [00-11] D 7 YLR382C NAM2 LeuRS|mitochondrial leucyl-tRNA synthetase|MSL1 Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance mitochondrial translation, RNA splicing, mitochondrion organization, cellular amino acid metabolic process, tRNA aminoacylation for protein translation RNA binding, ligase activity, mRNA binding mitochondrion, cytoplasm BY4742 [00-11] D 8 YLR394W CST9 ZIP3 SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate meiotic cell cycle, protein modification by small protein conjugation or removal, DNA recombination ligase activity nucleus, chromosome BY4742 [00-11] D 9 YLR406C RPL31B YL28|L34B|L31B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality cytoplasmic translation, regulation of translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-11] D 10 - - - - - - - - [00-11] D 11 YML022W APT1 - Adenine phosphoribosyltransferase, catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm BY4742 [00-11] D 12 - - - - - - - - [00-11] E 1 YML027W YOX1 - Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate mitotic cell cycle, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus, chromosome BY4742 [00-11] E 2 - - - - - - - - [00-11] E 3 - - - - - - - - [00-11] E 4 - - - - - - - - [00-11] E 5 YML036W CGI121 - Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification telomere organization, transcription from RNA polymerase II promoter molecular_function other BY4742 [00-11] E 6 YML038C YMD8 - Putative nucleotide sugar transporter, has similarity to Vrg4p transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [00-11] E 7 YML041C VPS71 SWC6 Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting protein targeting, chromatin organization other nucleus, chromosome BY4742 [00-11] E 8 YML042W CAT2 YCAT Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes cellular amino acid metabolic process transferase activity, transferring acyl groups peroxisome, mitochondrion, cytoplasm BY4742 [00-11] E 9 YML047C PRM6 - Pheromone-regulated protein, predicted to have 2 transmembrane segments; regulated by Ste12p during mating biological_process molecular_function membrane BY4742 [00-11] E 10 - - - - - - - - [00-11] E 11 - - - - - - - - [00-11] E 12 - - - - - - - - [00-11] F 1 YML075C HMG1 - One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; localizes to the nuclear envelope; overproduction induces the formation of karmellae cofactor metabolic process, lipid metabolic process oxidoreductase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-11] F 2 YML076C WAR1 - Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively response to chemical stimulus nucleic acid binding transcription factor activity nucleus, mitochondrion, cytoplasm BY4742 [00-11] F 3 - - - - - - - - [00-11] F 4 YML086C ALO1 - D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-11] F 5 - - - - - - - - [00-11] F 6 - - - - - - - - [00-11] F 7 - - - - - - - - [00-11] F 8 YMR048W CSM3 - Replication fork associated factor, required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [00-11] F 9 YMR135W-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-11] F 10 - - - - - - - - [00-11] F 11 YMR137C PSO2 SNM1 Nuclease required for a post-incision step in the repair of DNA single and double-strand breaks that result from interstrand crosslinks produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation response to DNA damage stimulus, DNA repair DNA binding, nuclease activity nucleus BY4742 [00-11] F 12 YMR138W CIN4 UGX1|GTP1 GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog protein folding other cytoplasm BY4742 [00-11] G 1 YMR139W RIM11 MDS1|GSK3 Protein kinase required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta protein phosphorylation, sporulation kinase activity cytoplasm BY4742 [00-11] G 2 YMR160W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-11] G 3 - - - - - - - - [00-11] G 4 YMR173W DDR48 FSP DNA damage-responsive protein, expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS response to DNA damage stimulus, DNA repair GTPase activity, ATPase activity cytoplasm BY4742 [00-11] G 5 - - - - - - - - [00-11] G 6 - - - - - - - - [00-11] G 7 YMR198W CIK1 - Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, chromosome segregation, conjugation, nucleus organization other cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-11] G 8 - - - - - - - - [00-11] G 9 YOR298C-A MBF1 SUF13 Transcriptional coactivator that bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus, mitochondrion, cytoplasm BY4742 [00-11] G 10 - - - - - - - - [00-11] G 11 - - - - - - - - [00-11] G 12 - - - - - - - - [00-11] H 1 YOR364W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C biological_process molecular_function cellular_component BY4742 [00-11] H 2 - - - - - - - - [00-11] H 3 - - - - - - - - [00-11] H 4 YPL148C PPT2 - Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation other other mitochondrion, cytoplasm BY4742 [00-11] H 5 - - - - - - - - [00-11] H 6 YPL183C RTT10 ERE2 WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endosome transport molecular_function cytoplasm BY4742 [00-11] H 7 YPL183W-A RTC6 TAE4|GON5 Protein involved translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein encoded in the nucleus mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-11] H 8 YPL189W GUP2 - Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog carbohydrate transport transferase activity, transferring acyl groups membrane BY4742 [00-11] H 9 - - - - - - - - [00-11] H 10 - - - - - - - - [00-11] H 11 YPL224C MMT2 MFT2 Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p cellular ion homeostasis molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-11] H 12 - - - - - - - - [00-12] A 1 YAL012W CYS3 STR1|FUN35|CYI1 Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-12] A 2 YAL016W TPD3 FUN32 Regulatory subunit A of the heterotrimeric protein phosphatase 2A (PP2A), which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III mitotic cell cycle, regulation of translation, organelle fission, cell budding, protein dephosphorylation, cytoskeleton organization, regulation of organelle organization, regulation of cell cycle phosphatase activity nucleus, cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm BY4742 [00-12] A 3 YAL047C SPC72 LDB4 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation, regulation of cell cycle structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-12] A 4 YAL054C ACS1 FUN44 Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions peptidyl-amino acid modification, protein acylation, cofactor metabolic process, chromatin organization, generation of precursor metabolites and energy, histone modification ligase activity mitochondrion, membrane, cytoplasm BY4742 [00-12] A 5 YAL056C-A - YAL058C-A Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] A 6 YAR050W FLO1 FLO4|FLO2 Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p other other cell wall BY4742 [00-12] A 7 YCL006C - - - - - - BY4742 [00-12] A 8 - - - - - - - - [00-12] A 9 - - - - - - - - [00-12] A 10 YCL022C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W biological_process molecular_function cellular_component BY4742 [00-12] A 11 YCL023C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 biological_process molecular_function cellular_component BY4742 [00-12] A 12 YCL038C ATG22 AUT4 Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation transmembrane transport, amino acid transport molecular_function membrane, vacuole, cytoplasm BY4742 [00-12] B 1 YCL058C FYV5 MDF1 Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis cellular ion homeostasis, conjugation, signaling, response to chemical stimulus molecular_function membrane BY4742 [00-12] B 2 YCL074W - - - - - - BY4742 [00-12] B 3 YCL075W - - - - - - BY4742 [00-12] B 4 YCL076W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] B 5 YGL199C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W biological_process molecular_function cellular_component BY4742 [00-12] B 6 YGL214W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C biological_process molecular_function cellular_component BY4742 [00-12] B 7 YGL217C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W biological_process molecular_function cellular_component BY4742 [00-12] B 8 YGL218W - SRF1 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component BY4742 [00-12] B 9 YGL235W - - Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component BY4742 [00-12] B 10 - - - - - - - - [00-12] B 11 YGR011W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] B 12 YGR018C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YGR017W biological_process molecular_function cellular_component BY4742 [00-12] C 1 YGR022C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W biological_process molecular_function cellular_component BY4742 [00-12] C 2 YGR025W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] C 3 - - - - - - - - [00-12] C 4 - - - - - - - - [00-12] C 5 YJR069C HAM1 - Conserved protein with deoxyribonucleoside triphosphate pyrophosphohydrolase activity, mediates exclusion of noncanonical purines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine response to DNA damage stimulus, nucleobase-containing small molecule metabolic process other nucleus, cytoplasm BY4742 [00-12] C 6 YJR070C LIA1 MMD1 Deoxyhypusine hydroxylase, a HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning peptidyl-amino acid modification, cytoskeleton organization, cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] C 7 - - - - - - - - [00-12] C 8 YJR074W MOG1 - Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p protein targeting, nuclear transport enzyme binding nucleus BY4742 [00-12] C 9 YJR077C MIR1 PTP Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-12] C 10 YJR080C AIM24 FMP26 Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-12] C 11 YJR084W - CSN12 Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome RNA splicing, conjugation, mRNA processing, signaling, response to chemical stimulus molecular_function chromosome BY4742 [00-12] C 12 - - - - - - - - [00-12] D 1 YJR087W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 biological_process molecular_function cellular_component BY4742 [00-12] D 2 YJR090C GRR1 SDC1|SSU2|COT2|CAT80 F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification mitotic cell cycle, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, response to DNA damage stimulus, protein modification by small protein conjugation or removal, conjugation, regulation of cell cycle protein binding, bridging, ligase activity nucleus, cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [00-12] D 3 YJR091C JSN1 PUF1 Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl RNA catabolic process RNA binding, mRNA binding membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-12] D 4 - - - - - - - - [00-12] D 5 YJR094C IME1 - Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p meiotic cell cycle, pseudohyphal growth, transcription from RNA polymerase II promoter, regulation of cell cycle protein binding transcription factor activity nucleus BY4742 [00-12] D 6 YJR094W-A RPL43B L43B Protein component of the large (60S) ribosomal subunit, identical to Rpl43Ap and has similarity to rat L37a ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-12] D 7 YJR095W SFC1 ACR1 Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-12] D 8 YJR096W - - Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] D 9 YJR097W JJJ3 DPH4 Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein peptidyl-amino acid modification molecular_function nucleus, cytoplasm BY4742 [00-12] D 10 YJR098C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-12] D 11 YJR099W YUH1 - Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p protein modification by small protein conjugation or removal peptidase activity cytoplasm BY4742 [00-12] D 12 YJR100C AIM25 - Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-12] E 1 YJR104C SOD1 CRS4 Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans response to oxidative stress, response to chemical stimulus, cellular ion homeostasis, cell wall organization or biogenesis oxidoreductase activity nucleus, mitochondrion, mitochondrial envelope, cytoplasm BY4742 [00-12] E 2 YJR106W ECM27 - Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p cell wall organization or biogenesis molecular_function membrane BY4742 [00-12] E 3 YJR107W - - Putative protein of unknown function; has sequence or structural similarity to lipases biological_process other cellular_component BY4742 [00-12] E 4 YJR109C CPA2 - Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor cellular amino acid metabolic process ligase activity cytoplasm BY4742 [00-12] E 5 - - - - - - - - [00-12] E 6 YJR113C RSM7 - Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-12] E 7 YJR116W TDA4 - Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function membrane BY4742 [00-12] E 8 YJR117W STE24 PIO2|AFC1 Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans protein maturation peptidase activity nucleus, endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [00-12] E 9 YJR118C ILM1 - Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth mitochondrion organization molecular_function membrane, endoplasmic reticulum, cytoplasm BY4742 [00-12] E 10 YJR119C JHD2 KDM5|KDM5 JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation chromatin organization, histone modification other nucleus, cytoplasm BY4742 [00-12] E 11 YJR120W - - Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p lipid transport, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration molecular_function cellular_component BY4742 [00-12] E 12 YJR121W ATP2 - Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-12] F 1 YJR122W IBA57 CAF17 Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system cofactor metabolic process molecular_function mitochondrion, cytoplasm BY4742 [00-12] F 2 - - - - - - - - [00-12] F 3 YJR124C - - Putative protein of unknown function; expression induced under calcium shortage biological_process molecular_function membrane BY4742 [00-12] F 4 YJR125C ENT3 - Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p cytoskeleton organization, Golgi vesicle transport, membrane invagination, endosome transport, endocytosis lipid binding cell cortex, cytoskeleton, endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm BY4742 [00-12] F 5 YJR126C VPS70 - Protein of unknown function involved in vacuolar protein sorting protein targeting molecular_function cellular_component BY4742 [00-12] F 6 YJR131W MNS1 - Alpha-1,2-mannosidase involved in ER quality control; catalyzes the removal of one mannose residue from Man9GlcNAc to produce a single isomer of Man8GlcNAc in N-linked oligosaccharide biosynthesis; integral to ER membrane protein glycosylation, carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process hydrolase activity, acting on glycosyl bonds endoplasmic reticulum, cytoplasm BY4742 [00-12] F 7 YJR133W XPT1 - Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups cytoplasm BY4742 [00-12] F 8 YJR134C SGM1 - Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus biological_process molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [00-12] F 9 YJR139C HOM6 - Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] F 10 YJR140C HIR3 HPC1 Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein mitotic cell cycle, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus BY4742 [00-12] F 11 - - - - - - - - [00-12] F 12 YJR142W - - Putative protein of unknown function; GST fusion protein is a Dbf2-Mob1 phosphoylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition biological_process molecular_function cellular_component BY4742 [00-12] G 1 YJR144W MGM101 MGM9 Protein with a role in mitochondrial DNA recombinational repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage response to DNA damage stimulus, DNA repair, DNA recombination DNA binding mitochondrion, cytoplasm BY4742 [00-12] G 2 YJR145C RPS4A rp5|YS6|S7A|S4A Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-12] G 3 YJR148W BAT2 ECA40|TWT2 Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase cellular amino acid metabolic process other nucleus, cytoplasm BY4742 [00-12] G 4 YJR150C DAN1 CCW13 Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth lipid transport molecular_function cell wall BY4742 [00-12] G 5 YJR153W PGU1 PSM1|PGL1 Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins carbohydrate metabolic process, pseudohyphal growth hydrolase activity, acting on glycosyl bonds extracellular region BY4742 [00-12] G 6 YKL005C BYE1 - Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit transcription from RNA polymerase II promoter histone binding nucleus BY4742 [00-12] G 7 YKL030W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1 biological_process molecular_function membrane BY4742 [00-12] G 8 - - - - - - - - [00-12] G 9 YLR146C SPE4 - Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm BY4742 [00-12] G 11 YLR286C CTS1 - Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p carbohydrate metabolic process, cytokinesis hydrolase activity, acting on glycosyl bonds nucleus, endomembrane system, cell wall, cellular bud, extracellular region, site of polarized growth, endoplasmic reticulum, cytoplasm BY4742 [00-12] G 10 - - - - - - - - [00-12] G 12 YLR343W GAS2 - 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p sporulation, cell wall organization or biogenesis other membrane, cytoplasm BY4742 [00-12] H 1 - - - - - - - - [00-12] H 2 - - - - - - - - [00-12] H 3 - - - - - - - - [00-12] H 4 YML067C ERV41 - Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus BY4742 [00-12] H 5 YML068W ITT1 - Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins regulation of translation molecular_function cellular_component BY4742 [00-12] H 6 YML072C TCB3 - Lipid-binding ER protein, enriched at ER-plasma membrane contact sites; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding cellular bud, cell cortex, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-12] H 7 - - - - - - - - [00-12] H 8 YMR136W GAT2 - Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine other nucleic acid binding transcription factor activity nucleus BY4742 [00-12] H 9 YMR172W HOT1 - Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity nucleus BY4742 [00-12] H 10 - - - - - - - - [00-12] H 11 YOR300W - HUF1 Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 cytokinesis molecular_function cellular_component BY4742 [00-12] H 12 YOR309C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 biological_process molecular_function cellular_component BY4742 [00-13] A 1 - - - - - - - - [00-13] A 2 - - - - - - - - [00-13] A 3 YJL003W COX16 - Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-13] A 4 YJL004C SYS1 - Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Golgi vesicle transport, endosome transport, vesicle organization molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-13] A 5 - - - - - - - - [00-13] A 6 YJL006C CTK2 - Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity transcription from RNA polymerase I promoter, regulation of translation, protein phosphorylation, transcription elongation, DNA-dependent, mRNA processing, translational elongation kinase activity nucleus, nucleolus BY4742 [00-13] A 7 - - - - - - - - [00-13] A 8 - - - - - - - - [00-13] A 9 - - - - - - - - [00-13] A 10 - - - - - - - - [00-13] A 11 - - - - - - - - [00-13] A 12 YJL012C VTC4 YJL012C-A|PHM3 Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion vacuole organization, membrane fusion, membrane invagination kinase activity vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-13] B 1 YJL013C MAD3 - Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle, chromosome segregation molecular_function nucleus BY4742 [00-13] B 2 - - - - - - - - [00-13] B 3 - - - - - - - - [00-13] B 4 YJL016W - YJL017W Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species biological_process molecular_function cytoplasm BY4742 [00-13] B 5 YJL017W - - - - - - BY4742 [00-13] B 6 - - - - - - - - [00-13] B 7 - - - - - - - - [00-13] B 8 YJL020C BBC1 YJL021C|MTI1 Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches cytoskeleton organization cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm BY4742 [00-13] B 9 YJL021C - - - - - - BY4742 [00-13] B 10 YJL022W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 biological_process molecular_function cellular_component BY4742 [00-13] B 11 YJL023C PET130 - Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-13] B 12 YJL024C APS3 YKS7 Small subunit of the clathrin-associated adaptor complex AP-3, which is involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function Golgi vesicle transport molecular_function membrane, cytoplasm BY4742 [00-13] C 1 - - - - - - - - [00-13] C 2 - - - - - - - - [00-13] C 3 YJL027C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-13] C 4 YJL028W - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome, cytoplasm BY4742 [00-13] C 5 YJL029C VPS53 - Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Golgi vesicle transport, endosome transport molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [00-13] C 6 YJR001W AVT1 - Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-13] C 7 - - - - - - - - [00-13] C 8 - - - - - - - - [00-13] C 9 YJR004C SAG1 AG(ALPHA)1 Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor conjugation, response to chemical stimulus other cell wall BY4742 [00-13] C 10 YJR005W APL1 YAP80 Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, plasma membrane, cytoplasm BY4742 [00-13] C 11 - - - - - - - - [00-13] C 12 - - - - - - - - [00-13] D 1 YJR008W - - Putative protein of unknown function; expression repressed by inosine and choline in an Opi1p-dependent manner; expression induced by mild heat-stress on a non-fermentable carbon source. biological_process molecular_function nucleus, cytoplasm BY4742 [00-13] D 2 YJR009C TDH2 GAPDH|GLD2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall carbohydrate metabolic process, generation of precursor metabolites and energy oxidoreductase activity cell wall, membrane fraction, mitochondrion, cytoplasm BY4742 [00-13] D 3 YJR010C-A SPC1 - Subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER), homolog of the SPC12 subunit of mammalian signal peptidase complex protein targeting, protein maturation molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-13] D 4 - - - - - - - - [00-13] D 5 YJR011C - - Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component BY4742 [00-13] D 6 - - - - - - - - [00-13] D 7 - - - - - - - - [00-13] D 8 YJR014W TMA22 RBF22 Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1 biological_process RNA binding ribosome, cytoplasm BY4742 [00-13] D 9 YJR015W - - Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene biological_process molecular_function membrane, endoplasmic reticulum, cytoplasm BY4742 [00-13] D 10 - - - - - - - - [00-13] D 11 - - - - - - - - [00-13] D 12 YJR018W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-13] E 1 YJR019C TES1 PTE1 Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids lipid metabolic process other peroxisome, mitochondrion, cytoplasm BY4742 [00-13] E 2 YJR020W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-13] E 3 YJR021C REC107 MER2 Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation meiotic cell cycle, DNA recombination molecular_function nucleus, chromosome BY4742 [00-13] E 4 - - - - - - - - [00-13] E 5 - - - - - - - - [00-13] E 6 YJR024C MDE1 - 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-13] E 7 YJR025C BNA1 HAD1 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity cytoplasm BY4742 [00-13] E 8 YJR026W - - - - - - BY4742 [00-13] E 9 - - - - - - - - [00-13] E 10 - - - - - - - - [00-13] E 11 - - - - - - - - [00-13] E 12 YJR030C - - Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; YJR030C is a non-essential gene biological_process molecular_function cellular_component BY4742 [00-13] F 1 - - - - - - - - [00-13] F 2 YJR032W CPR7 - Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity protein folding unfolded protein binding, isomerase activity cytoplasm BY4742 [00-13] F 3 YJR033C RAV1 SOI3 Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate cellular ion homeostasis, endosome transport, protein complex biogenesis molecular_function membrane, cytoplasm BY4742 [00-13] F 4 - - - - - - - - [00-13] F 5 YJR035W RAD26 - Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein response to DNA damage stimulus, DNA repair ATPase activity nucleus, cytoplasm BY4742 [00-13] F 6 YJR036C HUL4 - Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability protein modification by small protein conjugation or removal ligase activity cellular_component BY4742 [00-13] F 7 YJR037W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component BY4742 [00-13] F 8 YJR038C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-13] F 9 - - - - - - - - [00-13] F 10 YJR040W GEF1 CLC Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels cellular ion homeostasis transmembrane transporter activity vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, plasma membrane BY4742 [00-13] F 11 - - - - - - - - [00-13] F 12 - - - - - - - - [00-13] G 1 YJR043C POL32 - Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p DNA repair, DNA replication, response to DNA damage stimulus, RNA catabolic process, DNA recombination nucleotidyltransferase activity, nuclease activity nucleus, chromosome BY4742 [00-13] G 2 - - - - - - - - [00-13] G 3 - - - - - - - - [00-13] G 4 - - - - - - - - [00-13] G 5 YJR047C ANB1 eIF-5A|TIF51B|HYP1 Translation elongation factor eIF-5A, previously thought to function in translation initiation; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions regulation of translation, translational elongation RNA binding, translation factor activity, nucleic acid binding ribosome, cytoplasm BY4742 [00-13] G 6 YJR048W CYC1 iso-1-cytochrome c Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration generation of precursor metabolites and energy, cellular respiration other mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-13] G 7 YJR049C UTR1 - ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p) cofactor metabolic process, cellular ion homeostasis, nucleobase-containing small molecule metabolic process kinase activity nucleus, cytoplasm BY4742 [00-13] G 8 YJR050W ISY1 NTC30|UTR3 Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles RNA splicing, mRNA processing other nucleus BY4742 [00-13] G 9 YJR051W OSM1 FRDS2 Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-13] G 10 YJR052W RAD7 - Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex DNA repair, proteolysis involved in cellular protein catabolic process, response to DNA damage stimulus, protein modification by small protein conjugation or removal ligase activity, DNA binding, ATPase activity nucleus BY4742 [00-13] G 11 YJR053W BFA1 IBD1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis mitotic cell cycle, regulation of cell cycle enzyme regulator activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-13] G 12 - - - - - - - - [00-13] H 1 - - - - - - - - [00-13] H 2 YJR056C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [00-13] H 3 - - - - - - - - [00-13] H 4 YJR058C APS2 YAP17 Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, plasma membrane, cytoplasm BY4742 [00-13] H 5 YJR059W PTK2 STK2 Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake mitotic cell cycle, cellular ion homeostasis kinase activity nucleus, membrane, plasma membrane, cytoplasm BY4742 [00-13] H 6 YJR060W CBF1 CP1|CPF1|CEP1 Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation carbohydrate metabolic process, chromatin organization, chromosome segregation, cellular amino acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus, chromosome, mitochondrion, cytoplasm BY4742 [00-13] H 7 YJR061W - - Putative protein of unknown function; non-essential gene with similarity to Mnn4, a putative membrane protein involved in glycosylation; transcription repressed by Rm101p biological_process molecular_function cellular_component BY4742 [00-13] H 8 YJR062C NTA1 DEA1 Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds mitochondrion, cytoplasm BY4742 [00-13] H 9 YJR063W RPA12 A12.2|RRN4 RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex transcription from RNA polymerase I promoter, transcription termination, DNA-dependent molecular_function nucleus, nucleolus BY4742 [00-13] H 10 - - - - - - - - [00-13] H 11 - - - - - - - - [00-13] H 12 - - - - - - - - [00-14] A 1 YBR189W RPS9B rp21|YS11|S9B|S13|SUP46|RPS13A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins regulation of translation, rRNA processing, translational elongation, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-14] A 2 YCR095C OCA4 - Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm BY4742 [00-14] A 3 YCR102W-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] A 4 YDL133C-A RPL41B YL41|L47B|L41B|YDL134C-A|RPL47B Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Ap and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] A 5 - - - - - - - - [00-14] A 6 - - - - - - - - [00-14] A 7 YDR058C TGL2 - Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli lipid metabolic process other mitochondrion, cytoplasm BY4742 [00-14] A 8 - - - - - - - - [00-14] A 9 YDR174W HMO1 HSM2 Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase transcription from RNA polymerase I promoter, chromatin organization, transcription from RNA polymerase II promoter DNA binding nucleus, nucleolus, chromosome, cytoplasm BY4742 [00-14] A 10 YDR202C RAV2 - Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme cellular ion homeostasis, protein complex biogenesis molecular_function cytoplasm BY4742 [00-14] A 11 YDR205W MSC2 - Member of the cation diffusion facilitator family, localizes to the endoplasmic reticulum and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm BY4742 [00-14] A 12 YDR445C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] B 1 YDR537C - - Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W biological_process molecular_function cellular_component BY4742 [00-14] B 2 - - - - - - - - [00-14] B 3 YFR039C - - Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component BY4742 [00-14] B 4 YGL219C MDM34 MMM2 Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth mitochondrion organization, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [00-14] B 5 - - - - - - - - [00-14] B 6 YGR028W MSP1 YTA4 Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase protein targeting, mitochondrion organization ATPase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-14] B 7 YGR032W GSC2 FKS2 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) carbohydrate metabolic process, sporulation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups cell cortex, membrane fraction, cytoskeleton, membrane, cytoplasm, plasma membrane BY4742 [00-14] B 8 YGR038W ORM1 - Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control lipid metabolic process, response to chemical stimulus molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-14] B 9 YGR040W KSS1 - Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains protein phosphorylation, invasive growth in response to glucose limitation, response to chemical stimulus, conjugation, signaling kinase activity, signal transducer activity, transcription factor binding nucleus BY4742 [00-14] B 10 - - - - - - - - [00-14] B 11 - - - - - - - - [00-14] B 12 YGR050C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] C 1 YGR053C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-14] C 2 YGR063C SPT4 - Protein involved in the regulating Pol I and Pol II transcription, pre-mRNA processing, kinetochore function, and gene silencing; forms a complex with Spt5p transcription from RNA polymerase I promoter, DNA repair, chromatin organization, chromosome segregation, response to DNA damage stimulus, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus, chromosome BY4742 [00-14] C 3 - - - - - - - - [00-14] C 4 YGR086C PIL1 - Primary component of eisosomes, which are large immobile cell cortex structures associated with endocytosis; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family response to heat, protein phosphorylation, protein complex biogenesis, regulation of protein modification process, membrane invagination, endocytosis lipid binding cell cortex, membrane fraction, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-14] C 5 YGR089W NNF2 - Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation biological_process molecular_function membrane, membrane fraction BY4742 [00-14] C 6 YGR092W DBF2 - Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis protein phosphorylation, organelle fission, cellular ion homeostasis kinase activity cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm BY4742 [00-14] C 7 YGR093W - - Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus biological_process molecular_function nucleus BY4742 [00-14] C 8 YGR106C VOA1 - Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval protein complex biogenesis molecular_function membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm BY4742 [00-14] C 9 YGR110W CLD1 - Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 lipid metabolic process other mitochondrion, cytoplasm BY4742 [00-14] C 10 - - - - - - - - [00-14] C 11 YGR117C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-14] C 12 YGR238C KEL2 - Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate mitotic cell cycle, conjugation, regulation of cell cycle molecular_function cellular bud, site of polarized growth BY4742 [00-14] D 1 YGR239C PEX21 - Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex18p protein targeting, peroxisome organization other peroxisome, cytoplasm BY4742 [00-14] D 2 YGR248W SOL4 - 6-phosphogluconolactonase with similarity to Sol3p carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process other nucleus, cytoplasm BY4742 [00-14] D 3 YGR250C - - Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid biological_process RNA binding cytoplasm BY4742 [00-14] D 4 YJL129C TRK1 - Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation cellular ion homeostasis transmembrane transporter activity membrane BY4742 [00-14] D 5 YJL132W - - Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene biological_process molecular_function membrane fraction BY4742 [00-14] D 6 YJL136C RPS21B YS25|S26B|S21B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Ap and has similarity to rat S21 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] D 7 YJL137C GLG2 - Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups cytoplasm BY4742 [00-14] D 8 YJL139C YUR1 - Mannosyltransferase of the KTR1 family, involved in protein N-glycosylation; located in the Golgi apparatus protein glycosylation, carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus BY4742 [00-14] D 9 YJL140W RPB4 B32|CTF15 RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation response to heat, translational initiation, regulation of translation, nucleobase-containing compound transport, RNA catabolic process, transcription initiation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport RNA binding, nucleotidyltransferase activity, DNA binding nucleus, cytoplasm BY4742 [00-14] D 10 YJL141C YAK1 - Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose protein phosphorylation kinase activity nucleus, cytoplasm BY4742 [00-14] D 11 YJL151C SNA3 - Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles endosome transport enzyme binding membrane, vacuole, cytoplasm BY4742 [00-14] D 12 - - - - - - - - [00-14] E 1 YJL160C - - Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis biological_process molecular_function cellular_component BY4742 [00-14] E 2 YJL161W FMP33 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-14] E 3 YJL163C - - Putative protein of unknown function biological_process molecular_function membrane BY4742 [00-14] E 4 YJL165C HAL5 - Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters cellular ion homeostasis, protein dephosphorylation kinase activity cellular_component BY4742 [00-14] E 5 YJL172W CPS1 - Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions proteolysis involved in cellular protein catabolic process peptidase activity vacuole, cytoplasm BY4742 [00-14] E 6 - - - - - - - - [00-14] E 7 YJL175W - - Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor biological_process molecular_function membrane BY4742 [00-14] E 8 YJL177W RPL17B YL17|L20B|L17B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similarity to E. coli L22 and rat L17 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] E 9 YJL184W GON7 PCC2|LDB6 Component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Bud32p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; implicated in osmotic stress response telomere organization, cell wall organization or biogenesis, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome BY4742 [00-14] E 10 YJL189W RPL39 YL40|L46|L39|SPB2|RPL46|PUB2 Protein component of the large (60S) ribosomal subunit, has similarity to rat L39 ribosomal protein; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1 cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] E 11 YJL191W RPS14B rp59B|S14B|CRY2 Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, rRNA binding, structural molecule activity ribosome, cytoplasm BY4742 [00-14] E 12 - - - - - - - - [00-14] F 1 YJL196C ELO1 - Elongase I, medium-chain acyl elongase, catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids lipid metabolic process transferase activity, transferring acyl groups membrane BY4742 [00-14] F 2 YJL200C ACO2 - Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol biological_process lyase activity mitochondrion, cytoplasm BY4742 [00-14] F 3 - - - - - - - - [00-14] F 4 YJL206C - - Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment biological_process molecular_function cellular_component BY4742 [00-14] F 5 YJL213W - - Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p biological_process molecular_function ribosome, cytoplasm BY4742 [00-14] F 6 YKL096W-A CWP2 YKL097W-A|LPR1 Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored cell wall organization or biogenesis structural molecule activity cell wall BY4742 [00-14] F 7 YKL115C - - Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 biological_process molecular_function cellular_component BY4742 [00-14] F 8 YKL139W CTK1 - Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 transcription from RNA polymerase I promoter, peptidyl-amino acid modification, regulation of translation, protein phosphorylation, translational elongation, mRNA processing, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter kinase activity nucleus, nucleolus BY4742 [00-14] F 9 - - - - - - - - [00-14] F 10 - - - - - - - - [00-14] F 11 - - - - - - - - [00-14] F 12 YKL194C MST1 - Mitochondrial threonyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-14] G 1 - - - - - - - - [00-14] G 2 YKL201C MNN4 YKL200C Putative positive regulator of mannosylphosphate transferase (Mnn6p), involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases protein glycosylation, carbohydrate metabolic process molecular_function membrane BY4742 [00-14] G 3 YKL202W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] G 4 YKL204W EAP1 - eIF4E-associated protein, competes with eIF4G for binding to eIF4E; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade regulation of translation other cytoplasm BY4742 [00-14] G 5 - - - - - - - - [00-14] G 6 YKL215C OXP1 - 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm cellular amino acid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm BY4742 [00-14] G 7 YKL220C FRE2 - Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels transmembrane transport, ion transport oxidoreductase activity membrane, plasma membrane BY4742 [00-14] G 8 - - - - - - - - [00-14] G 9 YKR010C TOF2 - Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site mitotic cell cycle, organelle fission, chromosome segregation, nucleus organization enzyme regulator activity nucleus, mitochondrion, nucleolus, cytoplasm BY4742 [00-14] G 10 YKR019C IRS4 - EH domain-containing protein involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS response to starvation, cell wall organization or biogenesis, signaling molecular_function mitochondrion, cytoplasm BY4742 [00-14] G 11 YKR023W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-14] G 12 YKR027W BCH2 FMP50 Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane carbohydrate metabolic process, Golgi vesicle transport, cell wall organization or biogenesis molecular_function cytoplasmic membrane-bounded vesicle, mitochondrion, cytoplasm, Golgi apparatus BY4742 [00-14] H 1 YKR028W SAP190 - Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p mitotic cell cycle, tRNA processing, signaling, RNA modification molecular_function cytoplasm BY4742 [00-14] H 2 YKR029C SET3 - Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains meiotic cell cycle, chromatin organization, regulation of cell cycle, histone modification histone binding nucleus, chromosome BY4742 [00-14] H 3 YKR034W DAL80 UGA43 Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Deh1p transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-14] H 4 YKR036C CAF4 - WD40 repeat-containing protein associated with the CCR4-NOT complex, interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission organelle fission, mitochondrion organization, peroxisome organization, transcription from RNA polymerase II promoter molecular_function nucleus, mitochondrion, cytoplasm BY4742 [00-14] H 5 - - - - - - - - [00-14] H 6 YKR039W GAP1 - General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed cellular amino acid metabolic process, amino acid transport transmembrane transporter activity vacuole, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm, plasma membrane BY4742 [00-14] H 7 YKR040C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W biological_process molecular_function membrane BY4742 [00-14] H 8 YKR041W - - Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking biological_process molecular_function nucleus, cytoskeleton, cytoplasm BY4742 [00-14] H 9 YKR046C PET10 - Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange generation of precursor metabolites and energy, cellular respiration molecular_function cytoplasm BY4742 [00-14] H 10 YKR053C YSR3 LBP2 Dihydrosphingosine 1-phosphate phosphatase, membrane protein involved in sphingolipid metabolism; has similarity to Lcb3p lipid metabolic process phosphatase activity membrane, endoplasmic reticulum, cytoplasm BY4742 [00-14] H 11 YML035C AMD1 AMD3 AMP deaminase, tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; may be involved in regulation of intracellular adenine nucleotide pools nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm BY4742 [00-14] H 12 - - - - - - - - [00-15] A 1 - - - - - - - - [00-15] A 2 - - - - - - - - [00-15] A 3 - - - - - - - - [00-15] A 4 - - - - - - - - [00-15] A 5 - - - - - - - - [00-15] A 6 - - - - - - - - [00-15] A 7 - - - - - - - - [00-15] A 8 - - - - - - - - [00-15] A 9 - - - - - - - - [00-15] A 10 - - - - - - - - [00-15] A 11 - - - - - - - - [00-15] A 12 - - - - - - - - [00-15] B 1 YFL013W-A - - Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component BY4742 [00-15] B 2 YFL014W HSP12 HOR5|GLP1 Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; regulated by HOG and Ras-Pka pathways response to heat, response to oxidative stress, response to chemical stimulus, response to osmotic stress lipid binding nucleus, membrane fraction, membrane, cytoplasm, plasma membrane BY4742 [00-15] B 3 - - - - - - - - [00-15] B 4 YFL019C - - Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-15] B 5 - - - - - - - - [00-15] B 6 - - - - - - - - [00-15] B 7 - - - - - - - - [00-15] B 8 YFL042C - YFL043C Putative protein of unknown function; YFL042C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-15] B 9 - - - - - - - - [00-15] B 10 - - - - - - - - [00-15] B 11 - - - - - - - - [00-15] B 12 YFR019W FAB1 SVL7 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis lipid metabolic process kinase activity, lipid binding membrane, vacuole, mitochondrion, cytoplasm BY4742 [00-15] C 1 YFR024C - - - - - - BY4742 [00-15] C 2 YFR025C HIS2 - Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control cellular amino acid metabolic process phosphatase activity other BY4742 [00-15] C 3 - - - - - - - - [00-15] C 4 - - - - - - - - [00-15] C 5 YFR030W MET10 - Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide other oxidoreductase activity other BY4742 [00-15] C 6 - - - - - - - - [00-15] C 7 - - - - - - - - [00-15] C 8 - - - - - - - - [00-15] C 9 YHR146W CRP1 - Protein that binds to cruciform DNA structures biological_process DNA binding nucleus BY4742 [00-15] C 10 YHR171W ATG7 APG11|APG7|CVT2 Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation protein targeting, membrane invagination, protein modification by small protein conjugation or removal, response to starvation, protein lipidation other mitochondrion, membrane, cytoplasm BY4742 [00-15] C 11 - - - - - - - - [00-15] C 12 YJL042W MHP1 - Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins cytoskeleton organization, regulation of organelle organization, cell wall organization or biogenesis structural molecule activity cytoskeleton BY4742 [00-15] D 1 YJL070C - - Putative protein of unknown function with similarity to AMP deaminases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YJL070C is a non-essential gene biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-15] D 2 YJL078C PRY3 - Cell wall protein with a role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p biological_process molecular_function cell wall BY4742 [00-15] D 3 YJL094C KHA1 - Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies ion transport transmembrane transporter activity membrane, mitochondrion, cytoplasm, Golgi apparatus BY4742 [00-15] D 4 - - - - - - - - [00-15] D 5 YJL101C GSH1 - Gamma glutamylcysteine synthetase catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury response to oxidative stress, response to chemical stimulus, cellular amino acid metabolic process ligase activity other BY4742 [00-15] D 6 - - - - - - - - [00-15] D 7 YJL105W SET4 - Protein of unknown function, contains a SET domain biological_process molecular_function cellular_component BY4742 [00-15] D 8 - - - - - - - - [00-15] D 9 YJL128C PBS2 SSK4|SFS4|HOG4 MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition response to heat, protein targeting, protein phosphorylation, cytoskeleton organization, regulation of protein modification process, nuclear transport, signaling, response to osmotic stress kinase activity, structural molecule activity cellular bud, site of polarized growth, cytoplasm BY4742 [00-15] D 10 YKR085C MRPL20 YmL20 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-15] D 11 YKR094C RPL40B L40B|UB12|CEP52B|UBI2 Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes cytoplasmic translation, protein modification by small protein conjugation or removal structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-15] D 12 YKR095W MLP1 MPL1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs protein targeting, chromatin organization, nucleobase-containing compound transport, RNA catabolic process, transcription from RNA polymerase II promoter, nuclear transport other nucleus, cytoskeleton, microtubule organizing center, endomembrane system, membrane, cytoplasm BY4742 [00-15] E 1 YKR096W - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain biological_process molecular_function nucleus, ribosome, cytoplasm BY4742 [00-15] E 2 YKR102W FLO10 - Lectin-like protein with similarity to Flo1p, thought to be involved in flocculation other other cell wall BY4742 [00-15] E 3 YLR110C CCW12 - Cell wall mannoprotein with a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip conjugation, cell wall organization or biogenesis, response to chemical stimulus molecular_function cellular bud, cell wall, site of polarized growth BY4742 [00-15] E 4 YLR390W-A CCW14 ICWP|YLR391W|SSR1|YLR391W-A Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall cell wall organization or biogenesis structural molecule activity cell wall, mitochondrion, cytoplasm BY4742 [00-15] E 5 YLR439W MRPL4 YmL4 Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-15] E 6 YLR442C SIR3 STE8|MAR2|CMT1 Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p DNA repair, chromatin organization, response to DNA damage stimulus, RNA catabolic process histone binding, DNA binding, structural molecule activity nucleus, chromosome, mitochondrion, nucleolus, cytoplasm BY4742 [00-15] E 7 YLR455W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) biological_process molecular_function nucleus BY4742 [00-15] E 8 - - - - - - - - [00-15] E 9 YML066C SMA2 - Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation sporulation, cell wall organization or biogenesis molecular_function membrane, cytoplasm BY4742 [00-15] E 10 - - - - - - - - [00-15] E 11 - - - - - - - - [00-15] E 12 YML115C VAN1 LDB13|VRG8|VRG7 Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus BY4742 [00-15] F 1 YMR037C MSN2 - Transcriptional activator related to Msn4p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression response to heat, chromatin organization, response to oxidative stress, response to chemical stimulus, response to starvation, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-15] F 2 - - - - - - - - [00-15] F 3 YMR104C YPK2 YKR2 Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK protein phosphorylation, protein complex biogenesis kinase activity nucleus, cytoplasm BY4742 [00-15] F 4 - - - - - - - - [00-15] F 5 - - - - - - - - [00-15] F 6 YMR119W ASI1 - Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals proteolysis involved in cellular protein catabolic process ligase activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-15] F 7 YNR052C POP2 CAF1 RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation RNA catabolic process, mRNA processing, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter nuclease activity nucleus, site of polarized growth, cytoplasm BY4742 [00-15] F 8 YNR055C HOL1 - Putative transporter in the major facilitator superfamily (DHA1 family) of multidrug resistance transporters; mutations in membrane-spanning domains permit cation and histidinol uptake ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-15] F 9 YNR069C BSC5 - Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function cellular_component BY4742 [00-15] F 10 YOL125W TRM13 - 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases tRNA processing, RNA modification methyltransferase activity nucleus, cytoplasm BY4742 [00-15] F 11 - - - - - - - - [00-15] F 12 - - - - - - - - [00-15] G 1 YOL147C PEX11 PMP27|PMP24 Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p organelle fission, peroxisome organization, lipid metabolic process molecular_function peroxisome, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-15] G 2 - - - - - - - - [00-15] G 3 - - - - - - - - [00-15] G 4 - - - - - - - - [00-15] G 5 - - - - - - - - [00-15] G 6 - - - - - - - - [00-15] G 7 YPL268W PLC1 - Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes lipid metabolic process, signaling other nucleus, chromosome BY4742 [00-15] G 8 YPR007C REC8 SPO69 Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p meiotic cell cycle, DNA recombination, chromosome segregation molecular_function nucleus, chromosome BY4742 [00-15] G 9 YPR008W HAA1 - Transcriptional activator involved in the transcription of TPO2, YRO2, and other genes putatively encoding membrane stress proteins; involved in adaptation to weak acid stress transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity nucleus BY4742 [00-15] G 10 - - - - - - - - [00-15] G 11 - - - - - - - - [00-15] G 12 YPR013C - - Putative zinc finger protein; YPR013C is not an essential gene biological_process DNA binding cellular_component BY4742 [00-15] H 1 - - - - - - - - [00-15] H 2 YPR022C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process DNA binding nucleus, cytoplasm BY4742 [00-15] H 3 YPR023C EAF3 - Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, DNA replication, histone modification, response to DNA damage stimulus, transcription elongation, DNA-dependent, regulation of DNA metabolic process, transcription from RNA polymerase II promoter transferase activity, transferring acyl groups nucleus, chromosome BY4742 [00-15] H 4 YPR024W YME1 YTA11|OSD1 Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; mutation causes an elevated rate of mitochondrial turnover mitochondrion organization, proteolysis involved in cellular protein catabolic process peptidase activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-15] H 5 YPR026W ATH1 - Acid trehalase required for utilization of extracellular trehalose carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, acting on glycosyl bonds cell wall, vacuole, cytoplasm BY4742 [00-15] H 6 YPR031W NTO1 - Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification histone binding, transferase activity, transferring acyl groups nucleus, cytoplasm BY4742 [00-15] H 7 YPR037C ERV2 - Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER protein folding oxidoreductase activity membrane fraction BY4742 [00-15] H 8 YPR043W RPL43A L43A Protein component of the large (60S) ribosomal subunit, identical to Rpl43Bp and has similarity to rat L37a ribosomal protein; null mutation confers a dominant lethal phenotype cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-15] H 9 YPR050C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W biological_process molecular_function cellular_component BY4742 [00-15] H 10 YPR064W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-15] H 11 YPR067W ISA2 - Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations cofactor metabolic process, vitamin metabolic process ion binding mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-15] H 12 YPR078C - - Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible biological_process molecular_function cellular_component BY4742 [00-16] A 1 YAR002C-A ERP1 - Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, mitochondrion, cytoplasm BY4742 A 2 - - - - - - - - [00-16] A 3 YBR083W TEC1 ROC1 Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter, transposition protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-16] A 4 YBR084C-A RPL19A rpl5L|YL14|L23A|L19A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 A 5 - - - - - - - - A 6 - - - - - - - - [00-16] A 7 YBR090C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [00-16] A 8 YBR100W - - - - - - BY4742 [00-16] A 9 YBR112C CYC8 [OCT]|[OCT1+]|SSN6|CRT8 General transcriptional co-repressor, acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+] chromatin organization, regulation of transport, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus BY4742 A 10 - - - - - - - - [00-16] A 11 YBR125C PTC4 GCT1 Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity protein dephosphorylation phosphatase activity cytoplasm BY4742 [00-16] A 12 YBR131W CCZ1 CVT16 Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway membrane fusion, protein targeting, response to starvation, organelle fusion enzyme regulator activity membrane, cytoplasm BY4742 [00-16] B 1 YBR150C TBS1 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process DNA binding nucleus, mitochondrion, cytoplasm BY4742 [00-16] B 2 YBR168W PEX32 - Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function peroxisome, membrane, cytoplasm BY4742 [00-16] B 3 YBR169C SSE2 - Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; highly homologous to the heat shock protein Sse1p protein folding enzyme regulator activity cytoplasm BY4742 [00-16] B 4 YBR270C BIT2 - Subunit of TORC2, a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; similar to Bit61p biological_process molecular_function cellular_component BY4742 [00-16] B 5 YBR272C HSM3 - Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b response to DNA damage stimulus, DNA repair, protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [00-16] B 6 YBR275C RIF1 - Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation telomere organization DNA binding nucleus, chromosome BY4742 [00-16] B 7 YBR276C PPS1 - Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle mitotic cell cycle, protein phosphorylation, protein dephosphorylation, regulation of protein modification process, regulation of cell cycle, signaling phosphatase activity cellular_component BY4742 [00-16] B 8 YBR280C SAF1 - F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 proteolysis involved in cellular protein catabolic process ligase activity other BY4742 [00-16] B 9 YBR287W - ZSP1 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene biological_process molecular_function membrane, endoplasmic reticulum, cytoplasm BY4742 [00-16] B 10 YBR288C APM3 YKS6 Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Golgi vesicle transport molecular_function membrane, cytoplasm BY4742 [00-16] B 11 YBR289W SNF5 TYE4|SWI10|HAF4 Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p response to DNA damage stimulus, DNA repair, chromatin organization molecular_function nucleus BY4742 [00-16] B 12 YBR294W SUL1 SFP2 High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-16] C 1 YBR301W PAU24 DAN3 Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function cell wall BY4742 [00-16] C 2 YCL026C-A FRM2 YCLX08C Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis; expression induced in cells treated with the mycotoxin patulin; has similarity to bacterial nitroreductases lipid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-16] C 3 YCR028C-A RIM1 - Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication DNA replication, mitochondrion organization DNA binding mitochondrion, cytoplasm BY4742 [00-16] C 4 YCR030C SYP1 YCR029C-A Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization cytoskeleton organization molecular_function cellular bud, site of polarized growth BY4742 [00-16] C 5 YCR032W BPH1 - PProtein homologous to human Chediak-Higashi syndrome and murine Beige proteins, which are implicated in disease syndromes due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting cell wall organization or biogenesis molecular_function membrane fraction, mitochondrion, cytoplasm BY4742 [00-16] C 6 YCR033W SNT1 - Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance meiotic cell cycle, chromatin organization, histone modification, regulation of cell cycle, signaling molecular_function nucleus, chromosome BY4742 [00-16] C 7 YCR046C IMG1 - Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-16] C 8 YCR047C BUD23 - Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern rRNA processing, cytokinesis, ribosomal subunit export from nucleus, ribosomal small subunit biogenesis, nuclear transport, RNA modification methyltransferase activity nucleus, nucleolus, cytoplasm BY4742 [00-16] C 9 YCR048W ARE1 SAT2 Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen lipid metabolic process transferase activity, transferring acyl groups membrane, endoplasmic reticulum, cytoplasm BY4742 [00-16] C 10 YCR053W THR4 - Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway membrane invagination, endocytosis, cellular amino acid metabolic process lyase activity nucleus, cytoplasm BY4742 [00-16] C 11 - - - - - - - - [00-16] C 12 - - - - - - - - [00-16] D 1 YCR060W TAH1 - HSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2, contains a single TPR domain with at least two TPR motifs protein folding other nucleus, cytoplasm BY4742 [00-16] D 2 YCR062W - - - - - - BY4742 [00-16] D 3 - - - - - - - - [00-16] D 4 YCR067C SED4 - Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p regulation of organelle organization, regulation of transport, vesicle organization, Golgi vesicle transport, nucleobase-containing small molecule metabolic process enzyme regulator activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-16] D 5 YCR069W CPR4 SCC3|YCR070W|CYP4 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway biological_process isomerase activity membrane BY4742 [00-16] D 6 - - - - - - - - [00-16] D 7 YCR073C SSK22 - MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p signaling, response to osmotic stress kinase activity, signal transducer activity cellular_component BY4742 [00-16] D 8 YCR075C ERS1 - Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-16] D 9 YCR083W TRX3 - Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-16] D 10 YCR084C TUP1 UMR7|SFL2|ROX4|FLK1|CYC9|CRT4|AMM1|AER2|AAR1 General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes chromatin organization, regulation of transport, transcription from RNA polymerase II promoter small conjugating protein binding nucleus BY4742 [00-16] D 11 YCR088W ABP1 - Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization cytoskeleton organization cytoskeletal protein binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [00-16] D 12 YCR089W FIG2 - Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating cell morphogenesis, conjugation, response to chemical stimulus molecular_function cell wall BY4742 [00-16] E 1 YDL194W SNF3 - Plasma membrane low glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; similar to Rgt2p meiotic cell cycle, response to chemical stimulus, carbohydrate transport, regulation of cell cycle, signaling other membrane, plasma membrane BY4742 [00-16] E 2 YDR007W TRP1 - Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) cellular amino acid metabolic process isomerase activity cytoplasm BY4742 [00-16] E 3 - - - - - - - - [00-16] E 4 YDR048C - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-16] E 5 - - - - - - - - [00-16] E 6 YFR011C AIM13 MCS19 Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-16] E 7 YFR013W IOC3 - Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing chromatin organization DNA binding, ATPase activity nucleus BY4742 [00-16] E 8 - - - - - - - - [00-16] E 9 YNL051W COG5 COD4|API4 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting molecular_function cytoplasm, Golgi apparatus BY4742 [00-16] E 10 YNL052W COX5A - Subunit Va of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] E 11 YNL056W OCA2 - Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene biological_process molecular_function cytoplasm BY4742 [00-16] E 12 - - - - - - - - [00-16] F 1 YNL065W AQR1 - Plasma membrane multidrug transporter of the major facilitator superfamily, confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids transmembrane transport, amino acid transport, response to chemical stimulus transmembrane transporter activity membrane, plasma membrane BY4742 [00-16] F 2 YNL066W SUN4 SCW3 Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family mitochondrion organization hydrolase activity, acting on glycosyl bonds cell wall, mitochondrion, cytoplasm BY4742 [00-16] F 3 YNL067W RPL9B rp24|YL11|L9B|L8B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-16] F 4 YNL068C FKH2 - Forkhead family transcription factor with a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase mitotic cell cycle, chromatin organization, pseudohyphal growth, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding, transcription factor binding nucleus BY4742 [00-16] F 5 YNL070W TOM7 YOK22|MOM7 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] F 6 YNL071W LAT1 PDA2|ODP2 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA cofactor metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm BY4742 [00-16] F 7 YNL072W RNH201 Rnh2A|RNH35 Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome RNA catabolic process, DNA replication nuclease activity nucleus BY4742 [00-16] F 8 YNL073W MSK1 - Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, nucleobase-containing compound transport, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-16] F 9 YNL074C MLF3 YMK1 Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide response to chemical stimulus molecular_function cytoplasm BY4742 [00-16] F 10 YNL076W MKS1 LYS80 Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling transcription from RNA polymerase II promoter, signaling molecular_function cytoplasm BY4742 [00-16] F 11 YNL078W NIS1 JIP1 Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle molecular_function cellular bud, nucleus, site of polarized growth BY4742 [00-16] F 12 YNL079C TPM1 tropomyosin Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently vacuole organization, cell budding, mitochondrion organization, cytoskeleton organization, organelle inheritance, exocytosis, pseudohyphal growth cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [00-16] G 1 YNL080C EOS1 - Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus, protein glycosylation molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-16] G 2 YNL082W PMS1 - ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL response to DNA damage stimulus, meiotic cell cycle, DNA repair DNA binding, ATPase activity nucleus, cytoplasm BY4742 [00-16] G 3 YNL083W SAL1 - ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity, ion binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] G 4 YNL085W MKT1 - Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more response to DNA damage stimulus, regulation of translation nuclease activity cytoplasm BY4742 [00-16] G 5 YNL087W TCB2 - Bud-specific protein with a potential role in membrane trafficking; localizes to endoplasmic reticulum and is enriched at ER-plasma membrane contact sites; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud biological_process lipid binding cellular bud, cell cortex, endoplasmic reticulum, cytoplasm BY4742 [00-16] G 6 YNL090W RHO2 - Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly cytoskeleton organization, cell wall organization or biogenesis, signaling GTPase activity, signal transducer activity membrane fraction BY4742 [00-16] G 7 YNL091W NST1 - Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 response to osmotic stress molecular_function cytoplasm BY4742 [00-16] G 8 YNL093W YPT53 - Rab family GTPase, similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis protein targeting, membrane invagination, endocytosis GTPase activity cytoplasm BY4742 [00-16] G 9 YNL095C - - Putative protein of unknown function predicted to contain a transmembrane domain; YNL095C is not an essential gene biological_process molecular_function membrane BY4742 [00-16] G 10 YNL097C PHO23 - Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 transcription from RNA polymerase I promoter, response to heat, chromatin organization, transcription from RNA polymerase II promoter histone binding nucleus, chromosome BY4742 [00-16] G 11 YNL099C OCA1 - Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA response to oxidative stress, response to chemical stimulus phosphatase activity cytoplasm BY4742 [00-16] G 12 YNL100W AIM37 MCS27 Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-16] H 1 YNL104C LEU4 - Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway cellular amino acid metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm BY4742 [00-16] H 2 YNL105W RRT16 - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component BY4742 [00-16] H 3 YNL106C INP52 SJL2 Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain lipid metabolic process phosphatase activity cell cortex, site of polarized growth, membrane fraction, cytoskeleton, cytoplasm BY4742 [00-16] H 4 YNL107W YAF9 - Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain response to DNA damage stimulus, DNA repair, chromatin organization molecular_function nucleus, chromosome, cytoplasm BY4742 [00-16] H 5 YNL115C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-16] H 6 YNL119W NCS2 TUC2 Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA processing, invasive growth in response to glucose limitation, protein modification by small protein conjugation or removal, pseudohyphal growth, RNA modification molecular_function cytoplasm BY4742 [00-16] H 7 YNL120C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene biological_process molecular_function cellular_component BY4742 [00-16] H 8 YNL121C TOM70 OMP1|MOM72|MAS70 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] H 9 YNL125C ESBP6 MCH3 Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane other other membrane, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-16] H 10 - - - - - - - - [00-16] H 11 YNL130C CPT1 - Cholinephosphotransferase, required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription lipid metabolic process other membrane fraction, mitochondrion, membrane, mitochondrial envelope, Golgi apparatus, cytoplasm BY4742 [00-16] H 12 - - - - - - - - [00-17] A 1 YAL024C LTE1 MSI2 Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures mitotic cell cycle, regulation of cell cycle enzyme regulator activity cellular bud BY4742 [00-17] A 2 YBR299W MAL32 MALS|MAL3S Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, acting on glycosyl bonds membrane fraction BY4742 [00-17] A 3 - - - - - - - - [00-17] A 4 - - - - - - - - [00-17] A 5 YCR107W AAD3 - Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role other oxidoreductase activity cellular_component BY4742 [00-17] A 6 - - - - - - - - [00-17] A 7 - - - - - - - - [00-17] A 8 YDR242W AMD2 - Putative amidase biological_process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component BY4742 [00-17] A 9 YDR326C YSP2 - Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification other molecular_function mitochondrion, cytoplasm BY4742 [00-17] A 10 YDR417C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W biological_process molecular_function membrane BY4742 [00-17] A 11 YDR444W - - Putative protein of unknown function biological_process molecular_function cytoplasm BY4742 [00-17] A 12 YDR461W MFA1 - Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 conjugation, signaling, response to chemical stimulus other extracellular region BY4742 [00-17] B 1 YDR493W MZM1 AIM8|FMP36 Protein required for assembly of the cytochrome bc(1) complex; facilitates insertion of Rip1p into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; conserved in metazoans mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm BY4742 [00-17] B 2 - - - - - - - - [00-17] B 3 YDR500C RPL37B YL35|L43|L37B Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Ap and to rat L37 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-17] B 4 YDR502C SAM2 ETH2 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cellular_component BY4742 [00-17] B 5 YDR506C - - Possible membrane-localized protein biological_process molecular_function cellular_component BY4742 [00-17] B 6 YDR512C EMI1 - Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs sporulation, mitochondrion organization, transcription from RNA polymerase II promoter, protein complex biogenesis molecular_function cellular_component BY4742 [00-17] B 7 YDR515W SLF1 SRO99 RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts cellular ion homeostasis, regulation of translation RNA binding cytoplasm BY4742 [00-17] B 8 - - - - - - - - [00-17] B 9 YER089C PTC2 - Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint protein phosphorylation, protein dephosphorylation, response to chemical stimulus, response to DNA damage stimulus, regulation of protein modification process, signaling, response to osmotic stress, regulation of cell cycle phosphatase activity nucleus, cytoplasm BY4742 [00-17] B 10 YFL001W DEG1 HRM3|PUS3 tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability tRNA processing, RNA modification isomerase activity nucleus, cytoplasm BY4742 [00-17] B 11 - - - - - - - - [00-17] B 12 YFL003C MSH4 - Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein meiotic cell cycle, DNA recombination DNA binding nucleus, chromosome BY4742 [00-17] C 1 YFL004W VTC2 PHM1 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion vacuole organization, membrane fusion, membrane invagination molecular_function vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-17] C 2 - - - - - - - - [00-17] C 3 YFL007W BLM10 YFL006W Proteasome activator subunit; found in association with core particles, with and without the 19S regulatory particle; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 protein complex biogenesis molecular_function nucleus, membrane BY4742 [00-17] C 4 YFL010C WWM1 - WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 biological_process molecular_function nucleus, mitochondrion, cytoplasm BY4742 [00-17] C 5 YFL010W-A AUA1 YFL011W-A Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease amino acid transport molecular_function cellular_component BY4742 [00-17] C 6 YFL012W - - Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin biological_process molecular_function cellular_component BY4742 [00-17] C 7 YFL013C IES1 - Subunit of the INO80 chromatin remodeling complex chromatin organization molecular_function nucleus, chromosome BY4742 [00-17] C 8 YFL016C MDJ1 - Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones response to heat, mitochondrion organization, proteolysis involved in cellular protein catabolic process, protein folding enzyme regulator activity, unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 - [00-17] C 9 YFL033C RIM15 TAK1 Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase meiotic cell cycle, protein phosphorylation, response to oxidative stress, response to chemical stimulus, regulation of cell cycle kinase activity nucleus, cytoplasm BY4742 [00-17] C 10 - - - - - - - - [00-17] C 11 YGR122C-A - - Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W biological_process molecular_function cellular_component BY4742 [00-17] C 12 YGR162W TIF4631 eiF4G1 Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit translational initiation, ribosomal large subunit biogenesis RNA binding, translation factor activity, nucleic acid binding ribosome, mitochondrion, cytoplasm BY4742 [00-17] D 1 YGR252W GCN5 AAS104|KAT2|SWI9|ADA4 Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, histone binding, transferase activity, transferring acyl groups nucleus, chromosome BY4742 [00-17] D 2 YGR254W ENO1 enolase|HSP48 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose vacuole organization, carbohydrate metabolic process, regulation of organelle organization, generation of precursor metabolites and energy, membrane fusion lyase activity membrane fraction, vacuole, mitochondrion, cytoplasm BY4742 [00-17] D 3 YGR255C COQ6 - Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) cofactor metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-17] D 4 YGR257C MTM1 - Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor ion transport other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-17] D 5 YGR258C RAD2 - Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein response to DNA damage stimulus, DNA repair, transcription from RNA polymerase II promoter nuclease activity nucleus BY4742 [00-17] D 6 YGR271W SLH1 - Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses cytoplasmic translation, regulation of translation helicase activity ribosome, cytoplasm BY4742 [00-17] D 7 YGR272C - - - - - - BY4742 [00-17] D 8 YGR273C - - Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-17] D 9 YGR276C RNH70 RNA82|REX1 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts tRNA processing, rRNA processing nuclease activity nucleus BY4742 [00-17] D 10 YGR289C MAL11 MALT|AGT1 Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization carbohydrate metabolic process, carbohydrate transport, oligosaccharide metabolic process transmembrane transporter activity membrane, plasma membrane BY4742 [00-17] D 11 YGR291C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-17] D 12 YGR292W MAL12 MALS Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, acting on glycosyl bonds membrane fraction BY4742 [00-17] E 1 YGR295C COS6 - Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-17] E 2 YHR132W-A IGO2 - Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p other molecular_function nucleus, cytoplasm BY4742 [00-17] E 3 YIL030C SSM4 KIS3|DOA10 Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation proteolysis involved in cellular protein catabolic process ligase activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-17] E 4 - - - - - - - - [00-17] E 5 YIL058W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-17] E 6 YIL092W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [00-17] E 7 YIR023W DAL81 UGA35 Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism transcription from RNA polymerase II promoter, response to chemical stimulus, cellular amino acid metabolic process protein binding transcription factor activity, DNA binding nucleus BY4742 [00-17] E 8 YIR030C DCG1 - Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain other molecular_function cellular_component BY4742 [00-17] E 9 YIR032C DAL3 - Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds membrane BY4742 [00-17] E 10 YIR043C - - - - - - BY4742 [00-17] E 11 YIR044C - - - - - - BY4742 [00-17] E 12 YJR003C - - Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-17] F 1 - - - - - - - - [00-17] F 2 YJR055W HIT1 - Protein of unknown function, required for growth at high temperature biological_process molecular_function nucleus, cytoplasm BY4742 [00-17] F 3 YKL053C-A MDM35 - Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs mitochondrion organization, protein complex biogenesis molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-17] F 4 YKR106W GEX2 - Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-17] F 5 - - - - - - - - [00-17] F 6 YMR191W SPG5 - Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component BY4742 [00-17] F 7 YMR322C SNO4 HSP34 Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation biological_process peptidase activity cellular_component BY4742 [00-17] F 8 YNL138W SRV2 CAP CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis cytoskeleton organization, signaling enzyme binding, cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [00-17] F 9 YNL140C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene THO2/YNL139C biological_process molecular_function cellular_component BY4742 [00-17] F 10 YNL142W MEP2 - Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation pseudohyphal growth, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-17] F 11 YNL315C ATP11 - Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase mitochondrion organization, protein complex biogenesis unfolded protein binding mitochondrion, cytoplasm BY4742 [00-17] F 12 YOL151W GRE2 - 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway lipid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-17] G 1 YOL152W FRE7 - Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels transmembrane transport, ion transport oxidoreductase activity membrane, plasma membrane BY4742 [00-17] G 2 YOL155C HPF1 - Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds cell wall, extracellular region BY4742 [00-17] G 3 YOR265W RBL2 - Protein involved in microtubule morphogenesis, required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A protein folding, cytoskeleton organization, protein complex biogenesis cytoskeletal protein binding cytoplasm BY4742 [00-17] G 4 YOR266W PNT1 - Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-17] G 5 YOR267C HRK1 - Protein kinase implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis cellular ion homeostasis kinase activity cytoplasm BY4742 [00-17] G 6 YOR268C - - Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-17] G 7 YOR269W PAC1 - Protein involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; synthetic lethal with bni1; homolog of human LIS1 other cytoskeletal protein binding nucleus, cytoskeleton, cytoplasm BY4742 [00-17] G 8 YOR270C VPH1 - Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes cellular ion homeostasis, protein complex biogenesis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-17] G 9 YOR271C FSF1 - Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis biological_process molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-17] G 10 - - - - - - - - [00-17] G 11 YOR273C TPO4 - Polyamine transport protein, recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane; member of the major facilitator superfamily other transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-17] G 12 YOR274W MOD5 - Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase, required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms tRNA processing, RNA modification transferase activity, transferring alkyl or aryl (other than methyl) groups nucleus, nucleolus, mitochondrion, cytoplasm BY4742 [00-17] H 1 YOR275C RIM20 - Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation invasive growth in response to glucose limitation, sporulation, protein maturation molecular_function nucleus, cytoplasm BY4742 [00-17] H 2 YOR276W CAF20 p20|CAP20|CAF2 Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E regulation of translation other other BY4742 [00-17] H 3 YOR298C-A MBF1 SUF13 Transcriptional coactivator that bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus, mitochondrion, cytoplasm BY4742 [00-17] H 4 YOR302W - - CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation other cytoplasm BY4742 [00-17] H 5 YOR303W CPA1 - Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader cellular amino acid metabolic process ligase activity cytoplasm BY4742 [00-17] H 6 YPL004C LSP1 - Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family response to heat, protein phosphorylation, regulation of protein modification process, membrane invagination, endocytosis lipid binding cell cortex, membrane fraction, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-17] H 7 YPL017C IRC15 - Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation, DNA recombination, regulation of cell cycle methyltransferase activity, cytoskeletal protein binding cytoskeleton, cytoplasm BY4742 [00-17] H 8 YPL027W SMA1 - Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p sporulation molecular_function membrane BY4742 [00-17] H 9 YPL034W - - Putative protein of unknown function; YPL034W is not essential gene biological_process molecular_function cellular_component BY4742 [00-17] H 10 YPL036W PMA2 - Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential ion transport ATPase activity, transmembrane transporter activity membrane, membrane fraction, mitochondrion, plasma membrane, cytoplasm BY4742 [00-17] H 11 YPL078C ATP4 LPF7 Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-17] H 12 YPL137C GIP3 - Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments chromosome segregation enzyme binding, enzyme regulator activity ribosome, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-18] A 1 YBR020W GAL1 - Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p carbohydrate metabolic process, response to chemical stimulus kinase activity cytoplasm BY4742 [00-18] A 2 YBR075W - - - - - - BY4742 [00-18] A 3 YDR417C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W biological_process molecular_function membrane BY4742 [00-18] A 4 - - - - - - - - [00-18] A 5 - - - - - - - - [00-18] A 6 - - - - - - - - [00-18] A 7 - - - - - - - - [00-18] A 8 - - - - - - - - [00-18] A 9 - - - - - - - - [00-18] A 10 - - - - - - - - [00-18] A 11 - - - - - - - - [00-18] A 12 - - - - - - - - [00-18] B 1 - - - - - - - - [00-18] B 2 - - - - - - - - [00-18] B 3 - - - - - - - - [00-18] B 4 - - - - - - - - [00-18] B 5 - - - - - - - - [00-18] B 6 YFL033C RIM15 TAK1 Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase meiotic cell cycle, protein phosphorylation, response to oxidative stress, response to chemical stimulus, regulation of cell cycle kinase activity nucleus, cytoplasm BY4742 [00-18] B 7 - - - - - - - - [00-18] B 8 YFL063W - - Dubious open reading frame, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-18] B 9 - - - - - - - - [00-18] B 10 - - - - - - - - [00-18] B 11 YJL103C GSM1 - Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis generation of precursor metabolites and energy DNA binding cellular_component BY4742 [00-18] B 12 YML073C RPL6A rp18|YL16|L6A|L17A|YL16A N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal protein; binds to 5.8S rRNA cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-18] C 1 YNL011C - - Putative protein of unknown function; YNL011C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-18] C 2 YNL014W HEF3 ZRG7 Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells translational elongation RNA binding, translation factor activity, nucleic acid binding, ATPase activity ribosome, cytoplasm BY4742 [00-18] C 3 - - - - - - - - [00-18] C 4 YNL047C SLM2 LIT1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex endosome transport, cytoskeleton organization, signaling, protein complex biogenesis lipid binding membrane, plasma membrane BY4742 [00-18] C 5 YNL053W MSG5 - Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p protein targeting, protein phosphorylation, protein dephosphorylation, regulation of transport, response to chemical stimulus, regulation of protein modification process, conjugation, cell wall organization or biogenesis, nuclear transport, signaling phosphatase activity nucleus, cytoplasm BY4742 [00-18] C 6 YNL055C POR1 VDAC|YVDAC1|OMP2 Mitochondrial porin (voltage-dependent anion channel), outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated mitochondrion organization, ion transport, nucleobase-containing compound transport transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-18] C 7 YNL059C ARP5 - Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes chromatin organization helicase activity, ATPase activity nucleus, chromosome BY4742 [00-18] C 8 YNL069C RPL16B rp23|YL15|L21B|L16B|RP23 N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Ap, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-18] C 9 YNL086W SNN1 BLS1 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes biological_process molecular_function cytoplasm BY4742 [00-18] C 10 YNL089C - - Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins biological_process molecular_function cellular_component BY4742 [00-18] C 11 YNL096C RPS7B rp30|S7B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-18] C 12 - - - - - - - - [00-18] D 1 YNL109W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C biological_process molecular_function membrane BY4742 [00-18] D 2 YNL111C CYB5 - Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation lipid metabolic process other membrane, membrane fraction, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-18] D 3 YNL147W LSM7 - Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA RNA splicing, rRNA processing, ribosomal small subunit biogenesis, RNA catabolic process, mRNA processing RNA binding nucleus, nucleolus, cytoplasm BY4742 [00-18] D 4 YNL220W ADE12 BRA9 Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence nucleobase-containing small molecule metabolic process DNA binding, ligase activity cytoplasm BY4742 [00-18] D 5 YNL268W LYP1 - Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids amino acid transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-18] D 6 YNL284C MRPL10 YmL10|YmL18|MRPL18 Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-18] D 7 YNL315C ATP11 - Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase mitochondrion organization, protein complex biogenesis unfolded protein binding mitochondrion, cytoplasm BY4742 [00-18] D 8 - - - - - - - - [00-18] D 9 YNR033W ABZ1 - Para-aminobenzoate (PABA) synthase, has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis cellular amino acid metabolic process other cytoplasm BY4742 [00-18] D 10 YOL148C SPT20 ADA5 Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay peptidyl-amino acid modification, protein acylation, chromatin organization, response to chemical stimulus, histone modification, mRNA processing, signaling protein binding transcription factor activity nucleus BY4742 [00-18] D 11 YOL151W GRE2 - 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway lipid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-18] D 12 YOL152W FRE7 - Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels transmembrane transport, ion transport oxidoreductase activity membrane, plasma membrane BY4742 [00-18] E 1 - - - - - - - - [00-18] E 2 YPL158C AIM44 - Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular bud, site of polarized growth BY4742 [00-18] E 3 YPL183W-A RTC6 TAE4|GON5 Protein involved translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein encoded in the nucleus mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-18] E 4 YPL194W DDC1 - DNA damage checkpoint protein, part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate mitotic cell cycle, meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [00-18] E 5 - - - - - - - - [00-18] E 6 - - - - - - - - [00-18] E 7 YPR011C - - Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies other other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-18] E 8 YPR021C AGC1 - Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-18] E 9 YPR083W MDM36 - Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission organelle fission, mitochondrion organization molecular_function mitochondrion, cytoplasm BY4742 [00-18] E 10 - - - - - - - - [00-18] E 11 YPR091C NVJ2 - Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments biological_process lipid binding membrane, ribosome, endoplasmic reticulum, cytoplasm BY4742 [00-18] E 12 - - - - - - - - [00-18] F 1 YPR118W MRI1 - 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway cellular amino acid metabolic process isomerase activity nucleus, cytoplasm BY4742 [00-18] F 2 YPR133W-A TOM5 MOM8A Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore protein targeting, mitochondrion organization, protein complex biogenesis protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-18] F 3 YPR151C SUE1 - Mitochondrial protein required for degradation of unstable forms of cytochrome c other molecular_function mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-18] F 4 YMR074C - - Protein with homology to human PDCD5, which is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene other molecular_function nucleus, cytoplasm BY4742 [00-18] F 5 YMR118C SHH3 - Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase biological_process molecular_function membrane BY4742 [00-18] F 6 YOL153C - - - - - - BY4742 [00-18] F 7 - - - - - - - - [00-18] F 8 - - - - - - - - [00-18] F 9 - - - - - - - - [00-18] F 10 - - - - - - - - [00-18] F 11 - - - - - - - - [00-18] F 12 - - - - - - - - [00-18] G 1 - - - - - - - - [00-18] G 2 - - - - - - - - [00-18] G 3 - - - - - - - - [00-18] G 4 - - - - - - - - [00-18] G 5 - - - - - - - - [00-18] G 6 - - - - - - - - [00-18] G 7 - - - - - - - - [00-18] G 8 - - - - - - - - [00-18] G 9 - - - - - - - - [00-18] G 10 - - - - - - - - [00-18] G 11 - - - - - - - - [00-18] G 12 - - - - - - - - [00-18] H 1 - - - - - - - - [00-18] H 2 - - - - - - - - [00-18] H 3 - - - - - - - - [00-18] H 4 - - - - - - - - [00-18] H 5 - - - - - - - - [00-18] H 6 - - - - - - - - [00-18] H 7 - - - - - - - - [00-18] H 8 - - - - - - - - [00-18] H 9 - - - - - - - - [00-18] H 10 - - - - - - - - [00-18] H 11 - - - - - - - - [00-18] H 12 - - - - - - - - [1-1] A 1 - - - - - - - - [1-1] A 2 YAL068C PAU8 - Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component BY4739 [1-1] A 3 YAL067C SEO1 - Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide other other membrane BY4739 [1-1] A 4 YAL066W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4739 [1-1] A 5 YAL065C - - Putative protein of unknown function; has homology to FLO1; possible pseudogene biological_process molecular_function cellular_component BY4739 [1-1] A 6 - - - - - - - - [1-1] A 7 - - - - - - - - [1-1] A 8 - - - - - - - - [1-1] A 9 YAL062W GDH3 FUN51 NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources cellular amino acid metabolic process oxidoreductase activity nucleus, mitochondrion, cytoplasm BY4739 [1-1] A 10 YAL061W BDH2 - Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity nucleus, cytoplasm BY4742 [1-1] A 11 YAL060W BDH1 (2R,3R)-2,3-butanediol dehydrogenase|BDH NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source other oxidoreductase activity cytoplasm BY4739 [1-1] A 12 YAL059W ECM1 - Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm cell wall organization or biogenesis, ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus BY4739 [1-1] B 1 YAL058W CNE1 FUN48 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast protein folding, proteolysis involved in cellular protein catabolic process unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4739 [1-1] B 2 - - - - - - - - [1-1] B 3 YAL056W GPB2 KRH1 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; homolog of Gpb1p invasive growth in response to glucose limitation, sporulation, response to chemical stimulus, pseudohyphal growth, transcription from RNA polymerase II promoter, signaling enzyme regulator activity, signal transducer activity mitochondrion, membrane, cytoplasm, plasma membrane BY4739 [1-1] B 4 YAL055W PEX22 YAF5 Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation protein targeting, peroxisome organization, signaling other peroxisome, membrane, cytoplasm BY4739 [1-1] B 5 - - - - - - - - [1-1] B 6 YAL053W FLC2 HUF2 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance protein folding, transmembrane transport, cell wall organization or biogenesis, nucleobase-containing compound transport transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] B 7 YAL051W OAF1 YAF1 Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis lipid metabolic process, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus BY4739 [1-1] B 8 YAL049C AIM2 - Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor biological_process molecular_function cytoplasm BY4739 [1-1] B 9 - - - - - - - - [1-1] B 10 - - - - - - - - [1-1] B 11 - - - - - - - - [1-1] B 12 YAL045C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A biological_process molecular_function cellular_component BY4739 [1-1] C 1 YAL044C GCV3 - H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4739 [1-1] C 2 - - - - - - - - [1-1] C 3 YAL042W ERV46 FUN9 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus BY4739 [1-1] C 4 YAL043C-a - - - - - - BY4739 [1-1] C 5 - - - - - - - - [1-1] C 6 YAL040C CLN3 WHI1|FUN10|DAF1 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis vacuole organization, mitotic cell cycle, protein phosphorylation, membrane fusion, regulation of protein modification process, transcription from RNA polymerase II promoter, regulation of cell cycle enzyme regulator activity nucleus BY4739 [1-1] C 7 YAL039C CYC3 CCHL|cytochrome c heme lyase Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS) protein complex biogenesis lyase activity mitochondrial envelope, mitochondrion, cytoplasm BY4739 [1-1] C 8 - - - - - - - - [1-1] C 9 YAL037W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4739 [1-1] C 10 YAL036C RBG1 FUN11 Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid cytoplasmic translation other ribosome, cytoplasm BY4739 [1-1] C 11 YAL035W FUN12 eIF5B|yIF2 GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 translational initiation, regulation of translation RNA binding, GTPase activity, translation factor activity, nucleic acid binding ribosome, mitochondrion, cytoplasm BY4739 [1-1] C 12 - - - - - - - - [1-1] D 1 - - - - - - - - [1-1] D 2 YAL034C FUN19 - Non-essential protein of unknown function; expression induced in response to heat stress biological_process molecular_function cellular_component BY4739 [1-1] D 3 - - - - - - - - [1-1] D 4 - - - - - - - - [1-1] D 5 YAL031C GIP4 FUN21 Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate chromosome segregation enzyme binding, enzyme regulator activity cytoplasm BY4739 [1-1] D 6 YAL030W SNC1 - Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, membrane invagination, exocytosis, endocytosis other endomembrane system, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane BY4739 [1-1] D 7 YAL029C MYO4 SHE1|FUN22 One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p organelle inheritance cytoskeletal protein binding cellular bud, site of polarized growth, cytoskeleton, mitochondrion, cytoplasm BY4739 [1-1] D 8 YAL028W FRT2 HPH2 Tail-anchored ER membrane protein, interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate other molecular_function endoplasmic reticulum, cytoplasm BY4739 [1-1] D 9 YAL027W SAW1 - Protein involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus response to DNA damage stimulus, DNA repair, DNA recombination molecular_function nucleus BY4739 [1-1] D 10 YAL026C DRS2 SWA3|FUN38 Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease organelle assembly, ribosomal small subunit biogenesis, ribosome assembly, Golgi vesicle transport, lipid transport, membrane invagination, endocytosis ATPase activity membrane, Golgi apparatus, cytoplasm BY4739 [1-1] D 11 - - - - - - - - [1-1] D 12 - - - - - - - - [1-1] E 1 YAL023C PMT2 FUN25 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein Ser/Thr residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5; target for new antifungal carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] E 2 YAL022C FUN26 - Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4739 [1-1] E 3 YAL021C CCR4 NUT21|FUN27 Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening mitotic cell cycle, DNA replication, RNA catabolic process, transcription elongation, DNA-dependent, mRNA processing, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle nuclease activity nucleus, cytoplasm BY4739 [1-1] E 4 YAL020C ATS1 KTI13|FUN28 Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle tRNA processing, cell budding, cytoskeleton organization, RNA modification molecular_function cytoplasm BY4739 [1-1] E 5 YAL019W FUN30 - Conserved member of the Snf2p family with ATP-dependent chromatin remodeling activity; has a role in silencing; potential Cdc28p substrate; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies chromatin organization DNA binding, ATPase activity nucleus, chromosome, mitochondrion, cytoplasm BY4739 [1-1] E 6 YAL018C - - Putative protein of unknown function biological_process molecular_function membrane BY4739 [1-1] E 7 YAL017W PSK1 FUN31 One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity cytoplasm BY4739 [1-1] E 8 - - - - - - - - [1-1] E 9 YAL015C NTG1 ogg2|SCR1|FUN33 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress DNA repair, DNA replication, mitochondrion organization, regulation of organelle organization, response to oxidative stress, response to chemical stimulus, response to DNA damage stimulus, regulation of DNA metabolic process hydrolase activity, acting on glycosyl bonds, lyase activity nucleus, mitochondrion, cytoplasm BY4739 [1-1] E 10 YAL014C SYN8 SLT2|UIP2 Endosomal SNARE related to mammalian syntaxin 8 other other cytoplasm BY4739 [1-1] E 11 YAL013W DEP1 FUN54 Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation response to heat, lipid metabolic process, chromatin organization, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, histone modification molecular_function nucleus, chromosome BY4742 [1-1] E 12 - - - - - - - - [1-1] F 1 YAL011W SWC3 SWC1 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae chromatin organization molecular_function nucleus, chromosome, mitochondrion, cytoplasm BY4739 [1-1] F 2 YAL010C MDM10 FUN37 Subunit of both the ERMES complex that links the ER to mitochondria, and of the mitochondrial sorting and assembly machinery (SAM complex) that functions in import and assembly of outer membrane beta-barrel proteins protein targeting, mitochondrion organization, protein complex biogenesis, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4739 [1-1] F 3 YAL009W SPO7 - Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation meiotic cell cycle, lipid metabolic process, sporulation, nucleus organization phosphatase activity membrane, endomembrane system BY4739 [1-1] F 4 YAL008W FUN14 - Mitochondrial protein of unknown function biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4739 [1-1] F 5 YAL007C ERP2 - Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm BY4739 [1-1] F 6 YAL004W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C biological_process molecular_function cellular_component BY4739 [1-1] F 7 YAL005C SSA1 YG100 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall; 98% identical with Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes cytoplasmic translation, protein targeting, mitochondrion organization, transmembrane transport, protein folding, nuclear transport unfolded protein binding, ATPase activity nucleus, membrane fraction, vacuole, cell wall, membrane, cytoplasm BY4739 [1-1] F 8 - - - - - - - - [1-1] F 9 YAL002W VPS8 VPL8|VPT8|FUN15 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif protein targeting, endosome transport enzyme binding membrane fraction, cytoplasm BY4739 [1-1] F 10 - - - - - - - - [1-1] F 11 YAR002W NUP60 - Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; involved in nuclear export and cytoplasmic localization of specific mRNAs such as ASH1 response to heat, DNA repair, protein targeting, nucleobase-containing compound transport, response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport lipid binding, structural molecule activity nucleus, endomembrane system, membrane BY4739 [1-1] F 12 YAR003W SWD1 SAF49|CPS50|FUN16 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus BY4739 [1-1] G 1 - - - - - - - - [1-1] G 2 - - - - - - - - [1-1] G 3 - - - - - - - - [1-1] G 4 - - - - - - - - [1-1] G 5 YAR014C BUD14 - Protein involved in bud-site selection, Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cell morphogenesis, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis enzyme regulator activity cellular bud, site of polarized growth BY4739 [1-1] G 6 YAR015W ADE1 - N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process ligase activity nucleus, cytoplasm BY4739 [1-1] G 7 YAR018C KIN3 NPK1|FUN52 Nonessential serine/threonine protein kinase; possible role in DNA damage response chromosome segregation kinase activity cellular_component BY4739 [1-1] G 8 - - - - - - - - [1-1] G 9 YAR020C PAU7 - Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component BY4739 [1-1] G 10 YAR023C - - Putative integral membrane protein, member of DUP240 gene family biological_process molecular_function cellular_component BY4739 [1-1] G 11 YAR027W UIP3 - Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family biological_process molecular_function nucleus, endomembrane system BY4739 [1-1] G 12 YAR028W - - Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component BY4739 [1-1] H 1 YAR029W - - Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm BY4742 [1-1] H 2 YAR031W PRM9 - Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family vesicle organization molecular_function membrane, endoplasmic reticulum, plasma membrane, cytoplasm BY4739 [1-1] H 3 YAR030C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 biological_process molecular_function cellular_component BY4742 [1-1] H 4 - - - - - - - - [1-1] H 5 YAR035W YAT1 - Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated cellular amino acid metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm BY4739 [1-1] H 6 YAR037W - - - - - - BY4739 [1-1] H 7 YAR040C - - - - - - BY4739 [1-1] H 8 YAR042W SWH1 OSH1|YAR044W Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction lipid transport, membrane invagination, exocytosis, endocytosis lipid binding nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4739 [1-1] H 9 YAR043C - - - - - - BY4739 [1-1] H 10 YAR044W - - - - - - BY4739 [1-1] H 11 YAR047C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4739 [1-1] H 12 - - - - - - - - [2-1] A 1 YBL001C ECM15 - Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis cell wall organization or biogenesis molecular_function nucleus, cytoplasm BY4742 [2-1] A 2 YBL002W HTB2 - Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation chromatin organization DNA binding nucleus, chromosome BY4742 [2-1] A 3 YBL003C HTA2 H2A2 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p response to DNA damage stimulus, DNA repair, chromatin organization DNA binding nucleus, chromosome BY4742 [2-1] A 4 - - - - - - - - [2-1] A 5 YBL005W PDR3 TPE2|AMY2 Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity nucleus, cytoplasm BY4742 [2-1] A 6 - - - - - - - - [2-1] A 7 - - - - - - - - [2-1] A 8 YBL006C LDB7 RSC14 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions chromatin organization, transcription elongation, DNA-dependent, cell wall organization or biogenesis, transcription from RNA polymerase II promoter ATPase activity nucleus BY4742 [2-1] A 9 YBL007C SLA1 - Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains cytoskeleton organization, membrane invagination, cell wall organization or biogenesis, endocytosis protein binding, bridging, small conjugating protein binding nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [2-1] A 10 YBL008W HIR1 - Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus, chromosome BY4742 [2-1] A 11 YBL009W ALK2 - Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk1p and to mammalian haspins mitotic cell cycle, meiotic cell cycle, protein phosphorylation, organelle fission kinase activity cellular_component BY4742 [2-1] A 12 YBL010C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles biological_process molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm BY4742 [2-1] B 1 YBL011W SCT1 GAT2 Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed lipid metabolic process transferase activity, transferring acyl groups membrane, membrane fraction, endoplasmic reticulum, cytoplasm BY4742 [2-1] B 2 YBL012C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] B 3 YBL013W FMT1 methionyl-tRNA transformylase Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate translational initiation, tRNA aminoacylation for protein translation, cellular amino acid metabolic process other mitochondrion, cytoplasm BY4742 [2-1] B 4 - - - - - - - - [2-1] B 5 YBL015W ACH1 - Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth other other mitochondrion, cytoplasm BY4742 [2-1] B 6 YBL016W FUS3 DAC2 Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its degradation protein phosphorylation, invasive growth in response to glucose limitation, transposition, response to chemical stimulus, conjugation, signaling, regulation of cell cycle kinase activity, signal transducer activity nucleus, mitochondrion, site of polarized growth, cytoplasm BY4742 [2-1] B 7 YBL017C PEP1 VPT1|VPS10 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments protein targeting other membrane, cytoplasm, Golgi apparatus BY4742 [2-1] B 8 - - - - - - - - [2-1] B 9 YBL019W APN2 ETH1 Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII response to DNA damage stimulus, DNA repair lyase activity, nuclease activity nucleus BY4742 [2-1] B 10 - - - - - - - - [2-1] B 11 YBL021C HAP3 - Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, response to chemical stimulus, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [2-1] B 12 YBL022C PIM1 LON1 ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria proteolysis involved in cellular protein catabolic process, protein complex biogenesis peptidase activity, ATPase activity mitochondrion, cytoplasm BY4742 [2-1] C 1 - - - - - - - - [2-1] C 2 YBL024W NCL1 TRM4 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; similar to Nop2p and human proliferation associated nucleolar protein p120 tRNA processing, RNA modification methyltransferase activity nucleus BY4742 [2-1] C 3 YBL025W RRN10 - Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I transcription from RNA polymerase I promoter nucleic acid binding transcription factor activity, DNA binding nucleus, nucleolus BY4742 [2-1] C 4 - - - - - - - - [2-1] C 5 YBL027W RPL19B rpl5L|YL14|L23B|L19B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-1] C 6 YBL028C - - Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus, ribosome, cytoplasm BY4742 [2-1] C 7 YBL029W - - Non-essential protein of unknown function biological_process molecular_function nucleus, cytoplasm BY4742 [2-1] C 8 - - - - - - - - [2-1] C 9 YBL031W SHE1 - Mitotic spindle protein that interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure mitotic cell cycle, cytoskeleton organization molecular_function nucleus, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] C 10 YBL032W HEK2 KHD1 RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K telomere organization, DNA replication RNA binding, mRNA binding nucleus, chromosome, cytoplasm BY4742 Slow Growing [2-1] C 11 YBL033C RIB1 - GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway vitamin metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cytoplasm BY4742 [2-1] C 12 - - - - - - - - [2-1] D 1 - - - - - - - - [2-1] D 2 YBL036C - - Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process other cellular_component BY4742 [2-1] D 3 YBL037W APL3 - Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, plasma membrane, cytoplasm BY4742 [2-1] D 4 YBL038W MRPL16 YmL47 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity, transferase activity, transferring acyl groups ribosome, mitochondrion, cytoplasm BY4742 [2-1] D 5 YBL039C URA7 - Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis lipid metabolic process, nucleobase-containing small molecule metabolic process ligase activity cytoplasm BY4742 [2-1] D 6 - - - - - - - - [2-1] D 7 - - - - - - - - [2-1] D 8 YBL042C FUI1 - High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane BY4742 [2-1] D 9 YBL043W ECM13 - Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation cell wall organization or biogenesis molecular_function cellular_component BY4742 [2-1] D 10 YBL044W - - Putative protein of unknown function; YBL044W is not an essential protein biological_process molecular_function cellular_component BY4742 [2-1] D 11 YBL045C COR1 QCR1 Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-1] D 12 YBL046W PSY4 - Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 response to DNA damage stimulus, protein dephosphorylation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm BY4742 [2-1] E 1 YBL047C EDE1 BUD15 Key endocytic protein involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins response to chemical stimulus, membrane invagination, signaling, endocytosis small conjugating protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] E 2 YBL048W RRT1 - Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data biological_process molecular_function membrane BY4742 [2-1] E 3 YBL049W MOH1 - Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase biological_process molecular_function cellular_component BY4742 [2-1] E 4 - - - - - - - - [2-1] E 5 YBL051C PIN4 MDT1 Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage response to DNA damage stimulus, mitotic cell cycle, regulation of cell cycle molecular_function cytoplasm BY4742 "Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p" [2-1] E 6 YBL052C SAS3 KAT6 Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups nucleus BY4742 [2-1] E 7 YBL053W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] E 8 YBL054W TOD6 PBF1 PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor, similar to Dot6p; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manne transcription from RNA polymerase II promoter DNA binding nucleus, chromosome, cytoplasm BY4742 [2-1] E 9 YBL055C - Tat-D 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases response to oxidative stress, response to chemical stimulus nuclease activity cytoplasm BY4742 [2-1] E 10 YBL056W PTC3 - Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation protein phosphorylation, protein dephosphorylation, regulation of protein modification process, signaling, regulation of cell cycle, response to osmotic stress phosphatase activity nucleus, cytoplasm BY4742 [2-1] E 11 YBL057C PTH2 - One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-1] E 12 YBL058W SHP1 UBX1 UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate vacuole organization, mitotic cell cycle, carbohydrate metabolic process, organelle fission, sporulation, cytoskeleton organization, organelle assembly, proteolysis involved in cellular protein catabolic process, generation of precursor metabolites and energy, chromosome segregation, membrane invagination, response to starvation enzyme regulator activity nucleus, cytoplasm BY4742 Slow Growing [2-1] F 1 YBL059W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-1] F 2 YBL060W YEL1 - Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip lipid metabolic process enzyme regulator activity cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] F 3 YBL061C SKT5 CSD4|CHS4|CAL2 Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation carbohydrate metabolic process, cytokinesis, cell wall organization or biogenesis, response to osmotic stress enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] F 4 YBL062W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] F 5 YBL063W KIP1 CIN9 Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [2-1] F 6 YBL064C PRX1 - Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm BY4742 Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity [2-1] F 7 YBL065W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene biological_process molecular_function cellular_component BY4742 [2-1] F 8 YBL066C SEF1 - Putative transcription factor, has homolog in Kluyveromyces lactis biological_process molecular_function cellular_component BY4742 [2-1] F 9 YBL067C UBP13 - Putative ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions biological_process peptidase activity cellular_component BY4742 [2-1] F 10 YBL068W PRS4 - 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes carbohydrate metabolic process, cell wall organization or biogenesis other cytoplasm BY4742 [2-1] F 11 YBL069W AST1 - Peripheral membrane protein that interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane, possibly by influencing its incorporation into lipid rafts protein targeting molecular_function membrane BY4742 [2-1] F 12 YBL070C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] G 1 YBL071C - - Dubious open reading frame, predicted protein contains a peroxisomal targeting signal biological_process molecular_function cellular_component BY4742 [2-1] G 2 YBL072C RPS8A rp19|YS9|S8A|S14A Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-1] G 3 - - - - - - - - [2-1] G 4 - - - - - - - - [2-1] G 5 YBL075C SSA3 YG106 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm protein targeting, protein folding, transmembrane transport unfolded protein binding, ATPase activity cytoplasm BY4742 [2-1] G 6 - - - - - - - - [2-1] G 7 - - - - - - - - [2-1] G 8 YBL078C ATG8 AUT7|CVT5|APG8 Component of autophagosomes and Cvt vesicles; undergoes conjugation to phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation vacuole organization, protein targeting, organelle assembly, Golgi vesicle transport, membrane fusion, membrane invagination, response to starvation molecular_function vacuole, membrane, cytoplasm BY4742 [2-1] G 9 YBL079W NUP170 NLE3 Subunit of the nuclear pore complex (NPC), required for NPC localization of specific nucleoporins; involved in nuclear envelope permeability and chromosome segregation; has similar to Nup157p; essential role, with Nup157p, in NPC assembly protein targeting, nucleobase-containing compound transport, protein complex biogenesis, chromosome segregation, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane BY4742 [2-1] G 10 YBL080C PET112 - Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog mitochondrial translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [2-1] G 11 YBL081W - - Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function cellular_component BY4742 [2-1] G 12 YBL082C ALG3 RHK1 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins carbohydrate metabolic process, protein glycosylation, lipid metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm BY4742 [2-1] H 1 YBL083C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 biological_process molecular_function cellular_component BY4742 [2-1] H 2 - - - - - - - - [2-1] H 3 YBL085W BOI1 BOB1|GIN7 Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain cell budding, cytokinesis, signaling lipid binding cellular bud, site of polarized growth BY4742 [2-1] H 4 YBL086C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component BY4742 [2-1] H 5 YBL087C RPL23A YL32|L23A|L17aA Protein component of the large (60S) ribosomal subunit, identical to Rpl23Bp and has similarity to E. coli L14 and rat L23 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-1] H 6 YBL088C TEL1 - Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene DNA repair, telomere organization, protein phosphorylation, chromatin organization, histone modification, response to DNA damage stimulus kinase activity, DNA binding nucleus, mitochondrion, cytoplasm BY4742 [2-1] H 7 YBL089W AVT5 - Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters other other membrane BY4742 "Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters" [2-1] H 8 YBL090W MRP21 MRP50 Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [2-1] H 9 YBL091C MAP2 - Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p protein maturation peptidase activity cytoplasm BY4742 [2-1] H 10 - - - - - - - - [2-1] H 11 YBL093C ROX3 MED19|SSN7|NUT3 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus BY4742 Slow Growing [2-1] H 12 YBL094C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W biological_process molecular_function cellular_component BY4742 [2-2] A 1 YBL095W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-2] A 2 YBL096C - - Non-essential protein of unknown function biological_process molecular_function cellular_component BY4742 [2-2] A 3 - - - - - - - - [2-2] A 4 YBL098W BNA4 - Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] A 5 YBL099W ATP1 - Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] A 6 YBL100C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1 biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-2] A 7 YBL101C ECM21 ART2 Protein involved in regulating the endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements membrane invagination, cell wall organization or biogenesis, endocytosis enzyme binding cytoplasm BY4742 [2-2] A 8 - - - - - - - - [2-2] A 9 - - - - - - - - [2-2] A 10 YBL102W SFT2 - Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Golgi vesicle transport, endosome transport molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [2-2] A 11 YBL103C RTG3 - Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways protein targeting, response to chemical stimulus, transcription from RNA polymerase II promoter, nuclear transport, signaling nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus, cytoplasm BY4742 [2-2] A 12 YBL104C SEA4 YBL103C-A Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [2-2] B 1 - - - - - - - - [2-2] B 2 YBL106C SRO77 SNI2|SOP2 Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p Golgi vesicle transport, exocytosis molecular_function membrane, plasma membrane BY4742 [2-2] B 3 YBL107C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBL107C is not an essential gene biological_process molecular_function cytoplasm BY4742 [2-2] B 4 YBR001C NTH2 - Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, acting on glycosyl bonds mitochondrion, cytoplasm BY4742 [2-2] B 5 - - - - - - - - [2-2] B 6 YBR003W COQ1 - Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis cofactor metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups mitochondrion, cytoplasm BY4742 [2-2] B 7 - - - - - - - - [2-2] B 8 YBR005W RCR1 SSH6 Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane cell wall organization or biogenesis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-2] B 9 YBR006W UGA2 UGA5 Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm response to oxidative stress, response to chemical stimulus, cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [2-2] B 10 YBR007C DSF2 - Deletion suppressor of mpt5 mutation biological_process molecular_function cellular_component BY4742 [2-2] B 11 YBR008C FLR1 - Plasma membrane multidrug transporter of the major facilitator superfamily, involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin transmembrane transport, response to chemical stimulus transmembrane transporter activity membrane, plasma membrane BY4742 [2-2] B 12 YBR009C HHF1 - Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity protein alkylation, chromatin organization, sporulation, histone modification DNA binding nucleus, chromosome BY4742 [2-2] C 1 YBR010W HHT1 SIN2|BUR5 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation mitotic cell cycle, DNA repair, organelle fission, chromatin organization, sporulation, regulation of organelle organization, response to DNA damage stimulus, regulation of cell cycle DNA binding nucleus, chromosome BY4742 [2-2] C 2 - - - - - - - - [2-2] C 3 YBR012C - - Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p biological_process molecular_function cellular_component BY4742 [2-2] C 4 - - - - - - - - [2-2] C 5 - - - - - - - - [2-2] C 6 YBR013C - - Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component BY4742 [2-2] C 7 YBR014C GRX7 - Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; functional overlap with GRX6 response to oxidative stress, response to chemical stimulus oxidoreductase activity membrane, vacuole, cytoplasm, Golgi apparatus BY4742 [2-2] C 8 YBR015C MNN2 LDB8|CRV4|TTP1 Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus BY4742 [2-2] C 9 YBR016W - - Tail-anchored plasma membrane protein containing a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions biological_process molecular_function cellular bud, membrane, plasma membrane BY4742 [2-2] C 10 - - - - - - - - [2-2] C 11 YBR018C GAL7 - Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism carbohydrate metabolic process nucleotidyltransferase activity cytoplasm BY4742 [2-2] C 12 YBR019C GAL10 - UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers carbohydrate metabolic process isomerase activity other BY4742 [2-2] D 1 YBR020W GAL1 - Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p carbohydrate metabolic process, response to chemical stimulus kinase activity cytoplasm BY4742 [2-2] D 2 YBR021W FUR4 - Uracil permease, localized to the plasma membrane; expression is tightly regulated by uracil levels and environmental cues transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane BY4742 [2-2] D 3 YBR022W POA1 - Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing RNA splicing, tRNA processing phosphatase activity cellular_component BY4742 [2-2] D 4 YBR023C CHS3 KTI2|DIT101|CSD2|CAL1 Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan carbohydrate metabolic process, sporulation, cytokinesis, cell wall organization or biogenesis transferase activity, transferring glycosyl groups cellular bud, membrane, cytoplasmic membrane-bounded vesicle, site of polarized growth, cytoplasm BY4742 [2-2] D 5 YBR024W SCO2 - Protein anchored to the mitochondrial inner membrane, similar to Sco1p and may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p ion transport oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] D 6 YBR025C OLA1 - P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein levels are induced by hydrogen peroxide biological_process ATPase activity cytoplasm BY4742 [2-2] D 7 YBR026C ETR1 2-enoyl thioester reductase, E.C. 1.3.1.-|MRF1'|MRF1 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis lipid metabolic process, generation of precursor metabolites and energy, cellular respiration oxidoreductase activity mitochondrion, cytoplasm BY4742 [2-2] D 8 YBR027C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-2] D 9 YBR028C YPK3 - An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner biological_process kinase activity cytoplasm BY4742 [2-2] D 10 - - - - - - - - [2-2] D 11 YBR030W RKM3 - Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein peptidyl-amino acid modification, protein alkylation methyltransferase activity nucleus BY4742 [2-2] D 12 YBR031W RPL4A rp2|YL2|L4A|L2A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-2] E 1 YBR032W - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-2] E 2 YBR033W EDS1 - Putative zinc cluster protein; YBR033W is not an essential gene biological_process DNA binding cellular_component BY4742 [2-2] E 3 YBR034C HMT1 RMT1|ODP1|HCP1 Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p peptidyl-amino acid modification, protein alkylation, nucleobase-containing compound transport, transcription termination, DNA-dependent, transcription elongation, DNA-dependent, nuclear transport, transcription from RNA polymerase II promoter methyltransferase activity nucleus BY4742 [2-2] E 4 YBR035C PDX3 - Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism lipid metabolic process oxidoreductase activity cellular_component BY4742 [2-2] E 5 YBR036C CSG2 CLS2 Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations lipid metabolic process, cellular ion homeostasis enzyme regulator activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-2] E 6 YBR037C SCO1 PET161 Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins ion transport, response to chemical stimulus, protein complex biogenesis oxidoreductase activity, ion binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] E 7 - - - - - - - - [2-2] E 8 YBR039W ATP3 - Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] E 9 YBR040W FIG1 - Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating cell morphogenesis, conjugation, response to chemical stimulus molecular_function cell wall, membrane, site of polarized growth BY4742 [2-2] E 10 YBR041W FAT1 - Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids lipid metabolic process, lipid transport ligase activity peroxisome, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [2-2] E 11 YBR042C CST26 PSI1 Protein required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed lipid metabolic process transferase activity, transferring acyl groups cytoplasm BY4742 [2-2] E 12 YBR043C QDR3 AQR2 Multidrug transporter of the major facilitator superfamily, has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin transmembrane transport, response to chemical stimulus transmembrane transporter activity membrane, plasma membrane BY4742 [2-2] F 1 YBR044C TCM62 - Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone mitochondrion organization, protein complex biogenesis unfolded protein binding membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] F 2 YBR045C GIP1 - Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p sporulation, cell wall organization or biogenesis enzyme binding, enzyme regulator activity membrane, cytoplasm BY4742 [2-2] F 3 YBR046C ZTA1 - NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin response to oxidative stress, response to chemical stimulus RNA binding, oxidoreductase activity nucleus, cytoplasm BY4742 [2-2] F 4 YBR047W FMP23 - Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-2] F 5 YBR048W RPS11B rp41B|YS12|S18B|S11B Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [2-2] F 6 - - - - - - - - [2-2] F 7 YBR050C REG2 - Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease transcription from RNA polymerase II promoter enzyme regulator activity cytoplasm BY4742 [2-2] F 8 YBR051W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase biological_process molecular_function cellular_component BY4742 [2-2] F 9 YBR052C RFS1 - Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function membrane, cytoplasm BY4742 [2-2] F 10 YBR053C - - Putative protein of unknown function; induced by cell wall perturbation biological_process molecular_function cellular_component BY4742 [2-2] F 11 YBR054W YRO2 - Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p biological_process molecular_function cellular bud, membrane, mitochondrion, cytoplasm BY4742 [2-2] F 12 - - - - - - - - [2-2] G 1 YBR056W - - Putative cytoplasmic protein of unknown function biological_process molecular_function cytoplasm BY4742 [2-2] G 2 YBR057C MUM2 SPOT8 Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex DNA replication molecular_function cytoplasm BY4742 [2-2] G 3 YBR058C UBP14 GID6 Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T carbohydrate metabolic process, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process peptidase activity cytoplasm BY4742 [2-2] G 4 YBR059C AKL1 - Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization membrane invagination, cytoskeleton organization, regulation of transport, endocytosis kinase activity cellular bud, site of polarized growth, cytoplasm BY4742 [2-2] G 5 - - - - - - - - [2-2] G 6 - - - - - - - - [2-2] G 7 YBR062C - - Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. biological_process molecular_function cellular_component BY4742 [2-2] G 8 YBR063C - - Putative protein of unknown function; YBR063C is not an essential gene biological_process molecular_function cellular_component BY4742 [2-2] G 9 YBR064W - - Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C biological_process molecular_function cellular_component BY4742 [2-2] G 10 YBR065C ECM2 SLT11 Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p RNA splicing, mRNA processing molecular_function nucleus BY4742 [2-2] G 11 YBR066C NRG2 - Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter, response to osmotic stress, response to chemical stimulus DNA binding nucleus BY4742 [2-2] G 12 YBR067C TIP1 - Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins cell wall organization or biogenesis structural molecule activity cell wall BY4742 [2-2] H 1 YBR068C BAP2 - High-affinity leucine permease, functions as a branched-chain amino acid permease involved in the uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains transmembrane transport, amino acid transport transmembrane transporter activity membrane, plasma membrane BY4742 [2-2] H 2 YBR069C TAT1 VAP1|TAP1 Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance transmembrane transport, amino acid transport transmembrane transporter activity membrane, plasma membrane BY4742 [2-2] H 3 - - - - - - - - [2-2] H 4 YBR071W - - Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking biological_process molecular_function cellular bud, site of polarized growth, cytoplasm BY4742 [2-2] H 5 YBR072W HSP26 - Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity protein folding RNA binding, unfolded protein binding, mRNA binding nucleus, cytoplasm BY4742 [2-2] H 6 YBR073W RDH54 TID1 DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference meiotic cell cycle, DNA repair, chromosome segregation, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity nucleus BY4742 [2-2] H 7 YBR074W - YBR075W Putative metalloprotease biological_process molecular_function cellular_component BY4742 [2-2] H 8 YBR075W - - - - - - BY4742 [2-2] H 9 YBR076W ECM8 - Non-essential protein of unknown function cell wall organization or biogenesis molecular_function cellular_component BY4742 [2-2] H 10 YBR077C SLM4 GSE1|NIR1|EGO3 Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 membrane invagination, signaling lipid binding membrane, vacuole, cytoplasm BY4742 [2-2] H 11 YBR078W ECM33 - GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p cell wall organization or biogenesis molecular_function cell wall, membrane, membrane fraction, mitochondrion, plasma membrane, cytoplasm BY4742 [2-2] H 12 - - - - - - - - [2-3] A 1 - - - - - - - - [2-3] A 2 YBR081C SPT7 GIT2 Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex peptidyl-amino acid modification, protein acylation, chromatin organization, protein complex biogenesis, histone modification, conjugation structural molecule activity nucleus, mitochondrion, cytoplasm BY4742 [2-3] A 3 - - - - - - - - [2-3] A 4 - - - - - - - - [2-3] A 5 - - - - - - - - [2-3] A 6 YBR084W MIS1 - Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ligase activity, oxidoreductase activity mitochondrion, cytoplasm BY4742 [2-3] A 7 YBR085W AAC3 ANC3 Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration cofactor metabolic process, transmembrane transport, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-3] A 8 - - - - - - - - [2-3] A 9 - - - - - - - - [2-3] A 10 - - - - - - - - [2-3] A 11 - - - - - - - - [2-3] A 12 - - - - - - - - [2-3] B 1 YBR090C-A - - - - - - BY4742 [2-3] B 2 - - - - - - - - [2-3] B 3 YBR092C PHO3 phoC Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin vitamin metabolic process phosphatase activity other BY4742 [2-3] B 4 YBR093C PHO5 phoE Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 response to starvation phosphatase activity cell wall BY4742 [2-3] B 5 YBR094W PBY1 - Putative tubulin tyrosine ligase associated with P-bodies biological_process molecular_function cytoplasm BY4742 [2-3] B 6 YBR095C RXT2 RAF60 Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth invasive growth in response to glucose limitation, conjugation, transcription from RNA polymerase II promoter hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, chromosome BY4742 [2-3] B 7 YBR096W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER biological_process molecular_function endoplasmic reticulum, cytoplasm BY4742 [2-3] B 8 YBR097W VPS15 VPS40|VPL19|VAC4|GRD8 Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p vacuole organization, protein targeting, protein phosphorylation, peroxisome organization, endosome transport, organelle inheritance, response to starvation, signaling kinase activity, small conjugating protein binding endomembrane system, mitochondrion, membrane, Golgi apparatus, cytoplasm BY4742 [2-3] B 9 YBR098W MMS4 YBR100W|SLX2 Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination meiotic cell cycle, DNA repair, chromosome segregation, response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle protein binding transcription factor activity, nuclease activity nucleus BY4742 [2-3] B 10 YBR099C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 biological_process molecular_function cellular_component BY4742 [2-3] B 11 YBR100W - - - - - - BY4742 [2-3] B 12 YBR101C FES1 - Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum cytoplasmic translation enzyme regulator activity ribosome, cytoplasm BY4742 [2-3] C 1 - - - - - - - - [2-3] C 2 YBR103W SIF2 EMB1 WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus meiotic cell cycle, chromatin organization, histone modification, regulation of cell cycle, signaling molecular_function nucleus, chromosome BY4742 [2-3] C 3 YBR104W YMC2 - Mitochondrial protein, putative inner membrane transporter with a role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; has similarity with Ymc1p transmembrane transport transmembrane transporter activity membrane, mitochondrion, cytoplasm BY4742 [2-3] C 4 YBR105C VID24 GID4 Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase vacuole organization, carbohydrate metabolic process, protein targeting, organelle fusion, vesicle organization, proteolysis involved in cellular protein catabolic process, membrane fusion molecular_function cytoplasmic membrane-bounded vesicle, membrane, cytoplasm BY4742 [2-3] C 5 YBR106W PHO88 - Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations ion transport molecular_function membrane, mitochondrion, cytoplasm BY4742 [2-3] C 6 YBR107C IML3 MCM19 Protein with a role in kinetochore function, localizes to the outer kinetochore in a Ctf19p-dependent manner, interacts with Chl4p and Ctf19p mitotic cell cycle, meiotic cell cycle, organelle fission, sporulation, chromosome segregation molecular_function nucleus, chromosome BY4742 [2-3] C 7 YBR108W AIM3 - Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss cytoskeleton organization molecular_function membrane, cell cortex, cytoskeleton, cytoplasm BY4742 [2-3] C 8 - - - - - - - - [2-3] C 9 - - - - - - - - [2-3] C 10 YBR111C YSA1 RMA2 Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate carbohydrate metabolic process other nucleus, mitochondrion, cytoplasm BY4742 [2-3] C 11 - - - - - - - - [2-3] C 12 YBR113W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 biological_process molecular_function membrane BY4742 [2-3] D 1 YBR114W RAD16 PSO5 Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex DNA repair, proteolysis involved in cellular protein catabolic process, response to DNA damage stimulus, protein modification by small protein conjugation or removal ligase activity, DNA binding, ATPase activity nucleus BY4742 [2-3] D 2 YBR115C LYS2 - Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [2-3] D 3 YBR116C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 biological_process molecular_function cellular_component BY4742 [2-3] D 4 YBR117C TKL2 - Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process other nucleus, cytoplasm BY4742 [2-3] D 5 - - - - - - - - [2-3] D 6 YBR119W MUD1 U1A|U1-A U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing RNA splicing, mRNA processing RNA binding nucleus BY4742 [2-3] D 7 YBR120C CBP6 - Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other ribosome, mitochondrion, cytoplasm BY4742 [2-3] D 8 - - - - - - - - [2-3] D 9 - - - - - - - - [2-3] D 10 - - - - - - - - [2-3] D 11 - - - - - - - - [2-3] D 12 - - - - - - - - [2-3] E 1 YBR126C TPS1 TSS1|GLC6|GGS1|FDP1|CIF1|BYP1 Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups cytoplasm BY4742 [2-3] E 2 YBR127C VMA2 ATPSV|VAT2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm cellular ion homeostasis, transmembrane transport ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [2-3] E 3 YBR128C ATG14 APG14|CVT12 Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; homolog of human Barkor protein targeting, proteolysis involved in cellular protein catabolic process, peroxisome organization, membrane invagination, response to starvation kinase activity membrane fraction, vacuole, membrane, cytoplasm BY4742 [2-3] E 4 YBR129C OPY1 - Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-3] E 5 YBR130C SHE3 - Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance organelle inheritance RNA binding, mRNA binding cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [2-3] E 6 - - - - - - - - [2-3] E 7 YBR132C AGP2 - High affinity polyamine permease, preferentially uses spermidine over putrescine; expression is down-regulated by osmotic stress; plasma membrane carnitine transporter, also functions as a low-affinity amino acid permease transmembrane transport, ion transport, lipid metabolic process, amino acid transport, response to osmotic stress transmembrane transporter activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [2-3] E 8 YBR133C HSL7 - Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p mitotic cell cycle, regulation of cell cycle methyltransferase activity cellular bud, site of polarized growth BY4742 [2-3] E 9 YBR134W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-3] E 10 - - - - - - - - [2-3] E 11 - - - - - - - - [2-3] E 12 YBR137W - - Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene biological_process molecular_function cytoplasm BY4742 [2-3] F 1 YBR138C - HDR1 Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene biological_process molecular_function cytoplasm BY4742 [2-3] F 2 YBR139W - - Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner other peptidase activity vacuole, cytoplasm BY4742 [2-3] F 3 - - - - - - - - [2-3] F 4 YBR141C - - Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene biological_process methyltransferase activity nucleus, nucleolus BY4742 [2-3] F 5 - - - - - - - - [2-3] F 6 - - - - - - - - [2-3] F 7 YBR144C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene biological_process molecular_function cellular_component BY4742 [2-3] F 8 YBR145W ADH5 - Alcohol dehydrogenase isoenzyme V; involved in ethanol production carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [2-3] F 9 YBR146W MRPS9 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [2-3] F 10 YBR147W RTC2 RRT11 Protein of unknown function; mutant produces large lipid droplets, is resistant to fluconazole, has decreased levels of rDNA transcription, growth defects on minimal media, and suppresses cdc13-1; detected in highly purified mitochondria; similar to a G-protein coupled receptor from S. pombe biological_process molecular_function membrane, mitochondrion, cytoplasm BY4742 [2-3] F 11 YBR148W YSW1 - Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane sporulation molecular_function membrane BY4742 [2-3] F 12 YBR149W ARA1 - NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product carbohydrate metabolic process oxidoreductase activity cytoplasm BY4742 [2-3] G 1 - - - - - - - - [2-3] G 2 YBR151W APD1 - Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [2-3] G 3 - - - - - - - - [2-3] G 4 - - - - - - - - [2-3] G 5 - - - - - - - - [2-3] G 6 - - - - - - - - [2-3] G 7 YBR156C SLI15 - Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly protein phosphorylation, cytokinesis, chromosome segregation enzyme regulator activity cytoskeleton BY4742 [2-3] G 8 YBR157C ICS2 - Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization biological_process molecular_function cellular_component BY4742 [2-3] G 9 YBR158W AMN1 CST13|ICS4 Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) mitotic cell cycle, regulation of cell cycle other cellular bud, nucleus, cytoplasm BY4742 [2-3] G 10 YBR159W IFA38 - Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm BY4742 [2-3] G 11 - - - - - - - - [2-3] G 12 YBR161W CSH1 - Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p lipid metabolic process transferase activity, transferring glycosyl groups membrane, vacuole, cytoplasm BY4742 [2-3] H 1 YBR162C TOS1 - Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C biological_process molecular_function cell wall, vacuole, cytoplasm BY4742 [2-3] H 2 YBR162W-A YSY6 - Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion other molecular_function endoplasmic reticulum, cytoplasm BY4742 [2-3] H 3 YBR163W EXO5 DEM1 Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 mitochondrion organization nuclease activity mitochondrion, cytoplasm BY4742 [2-3] H 4 YBR164C ARL1 DLP2 Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Golgi vesicle transport, protein targeting GTPase activity cytoplasm, Golgi apparatus BY4742 [2-3] H 5 YBR165W UBS1 - Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity protein targeting, protein modification by small protein conjugation or removal, nuclear transport molecular_function nucleus BY4742 [2-3] H 6 YBR166C TYR1 - Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [2-3] H 7 - - - - - - - - [2-3] H 8 - - - - - - - - [2-3] H 9 - - - - - - - - [2-3] H 10 YBR170C NPL4 HRD4 Ubiquitin-binding protein involved in protein degradation; Cdc48p-Npl4p-Ufd1p complex participates in ER-associated degradation (ERAD) while Cdc48p-Npl4p-Vms1p complex participates in mitochondria-associated degradation (MAD) proteolysis involved in cellular protein catabolic process, response to chemical stimulus small conjugating protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] H 11 YBR171W SEC66 SEC71|HSS1 Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER protein targeting, transmembrane transport protein transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] H 12 YBR172C SMY2 - Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; has similarity to S. pombe Mpd2 Golgi vesicle transport molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-4] A 1 YBR173C UMP1 RNS2 Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [2-4] A 2 YBR174C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective biological_process molecular_function cellular_component BY4742 [2-4] A 3 YBR175W SWD3 SAF35|CPS30 Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus BY4742 [2-4] A 4 YBR176W ECM31 - Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process other mitochondrion, cytoplasm BY4742 [2-4] A 5 YBR177C EHT1 - Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane lipid metabolic process transferase activity, transferring acyl groups membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-4] A 6 YBR178W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C biological_process molecular_function cellular_component BY4742 [2-4] A 7 YBR179C FZO1 - Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system mitochondrion organization, organelle fusion, membrane fusion GTPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] A 8 YBR180W DTR1 - Putative dityrosine transporter, required for spore wall synthesis; expressed during sporulation; member of the major facilitator superfamily (DHA1 family) of multidrug resistance transporters sporulation, transmembrane transport, cell wall organization or biogenesis transmembrane transporter activity membrane BY4742 [2-4] A 9 YBR181C RPS6B RPS102|RPS101|LPG18 Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-4] A 10 YBR182C SMP1 - Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [2-4] A 11 YBR183W YPC1 alkaline ceramidase Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance lipid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, transferase activity, transferring acyl groups, hydrolase activity, acting on glycosyl bonds membrane, endoplasmic reticulum, cytoplasm BY4742 [2-4] A 12 YBR184W - - Putative protein of unknown function; YBR184W is not an essential gene biological_process molecular_function cellular_component BY4742 [2-4] B 1 YBR185C MBA1 - Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] B 2 YBR186W PCH2 - Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA meiotic cell cycle, regulation of cell cycle molecular_function nucleus, nucleolus BY4742 [2-4] B 3 YBR187W GDT1 - Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [2-4] B 4 YBR188C NTC20 - Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs RNA splicing, mRNA processing other nucleus BY4742 [2-4] B 5 - - - - - - - - [2-4] B 6 - - - - - - - - [2-4] B 7 - - - - - - - - [2-4] B 8 - - - - - - - - [2-4] B 9 - - - - - - - - [2-4] B 10 YBR194W AIM4 SOY1 Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress biological_process molecular_function cytoplasm BY4742 [2-4] B 11 YBR195C MSI1 CAC3 Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase chromatin organization histone binding nucleus, cytoplasm BY4742 [2-4] B 12 - - - - - - - - [2-4] C 1 YBR197C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [2-4] C 2 - - - - - - - - [2-4] C 3 YBR199W KTR4 - Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus BY4742 [2-4] C 4 YBR200W BEM1 SRO1 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p cell morphogenesis, conjugation, response to chemical stimulus structural molecule activity, lipid binding cellular bud, site of polarized growth BY4742 [2-4] C 5 YBR201W DER1 - Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-4] C 6 - - - - - - - - [2-4] C 7 YBR203W COS111 - Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signaling molecular_function mitochondrion, cytoplasm BY4742 [2-4] C 8 YBR204C LDH1 - Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) other other cytoplasm BY4742 [2-4] C 9 YBR205W KTR3 - Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles protein glycosylation, carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups vacuole, cytoplasm BY4742 [2-4] C 10 YBR206W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 biological_process molecular_function cellular_component BY4742 [2-4] C 11 YBR207W FTH1 - Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis transmembrane transport, ion transport, membrane invagination, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm BY4742 [2-4] C 12 YBR208C DUR1,2 DUR80 Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ligase activity cytoplasm BY4742 [2-4] D 1 YBR209W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene biological_process molecular_function cellular_component BY4742 [2-4] D 2 YBR210W ERV15 - Protein involved in export of proteins from the endoplasmic reticulum, has similarity to Erv14p Golgi vesicle transport, cell budding, cytokinesis molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm BY4742 [2-4] D 3 - - - - - - - - [2-4] D 4 YBR212W NGR1 RBP1 RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase mitochondrion organization RNA binding, mRNA binding cytoplasm BY4742 [2-4] D 5 YBR213W MET8 - Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis cofactor metabolic process lyase activity, oxidoreductase activity cellular_component BY4742 [2-4] D 6 YBR214W SDS24 - One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; may play an indirect role in fluid-phase endocytosis membrane invagination, cytokinesis, endocytosis molecular_function cytoplasm BY4742 [2-4] D 7 YBR215W HPC2 - Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes mitotic cell cycle, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter DNA binding nucleus BY4742 [2-4] D 8 YBR216C YBP1 - Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress response to oxidative stress, response to chemical stimulus molecular_function cytoplasm BY4742 [2-4] D 9 YBR217W ATG12 APG12 Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation vacuole organization, protein targeting, organelle assembly, membrane invagination, response to starvation, protein lipidation ligase activity membrane fraction, cytoplasm BY4742 [2-4] D 10 YBR218C PYC2 - Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process ligase activity cytoplasm BY4742 [2-4] D 11 YBR219C - - Putative protein of unknown function; YBR219C is not an essential gene biological_process molecular_function membrane BY4742 [2-4] D 12 YBR220C - - Putative protein of unknown function; YBR220C is not an essential gene biological_process molecular_function membrane BY4742 [2-4] E 1 YBR221C PDB1 - E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria cofactor metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [2-4] E 2 YBR222C PCS60 FAT2 Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase biological_process RNA binding, mRNA binding peroxisome, membrane, cytoplasm BY4742 [2-4] E 3 YBR223C TDP1 - Tyrosyl-DNA Phosphodiesterase I, hydrolyzes 3' and 5'-phosphotyrosyl bonds, involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI response to DNA damage stimulus, DNA repair other nucleus BY4742 [2-4] E 4 YBR224W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 biological_process molecular_function membrane BY4742 [2-4] E 5 YBR225W - - Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components biological_process molecular_function cellular_component BY4742 [2-4] E 6 YBR226C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W biological_process molecular_function cellular_component BY4742 [2-4] E 7 YBR227C MCX1 - Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein folding molecular_function mitochondrion, cytoplasm BY4742 [2-4] E 8 YBR228W SLX1 - Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA replication nuclease activity nucleus, chromosome BY4742 [2-4] E 9 YBR229C ROT2 GLS2 Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [2-4] E 10 YBR230C OM14 - Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-4] E 11 YBR231C SWC5 AOR1 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin organization molecular_function nucleus, chromosome BY4742 [2-4] E 12 - - - - - - - - [2-4] F 1 YBR233W PBP2 HEK1 RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length telomere organization RNA binding, mRNA binding cytoplasm BY4742 [2-4] F 2 - - - - - - - - [2-4] F 3 YBR235W - - Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene transmembrane transport transmembrane transporter activity membrane BY4742 [2-4] F 4 - - - - - - - - [2-4] F 5 - - - - - - - - [2-4] F 6 YBR238C - - Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span generation of precursor metabolites and energy, cellular respiration molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-4] F 7 YBR239C ERT1 - Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition biological_process DNA binding nucleus, cytoplasm BY4742 [2-4] F 8 YBR240C THI2 phoF|PHO6 Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type vitamin metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus BY4742 [2-4] F 9 YBR241C - - Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [2-4] F 10 YBR242W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [2-4] F 11 - - - - - - - - [2-4] F 12 YBR244W GPX2 AMI1 Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] G 1 YBR245C ISW1 SGN2 Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation transcription from RNA polymerase I promoter, chromatin organization, protein complex biogenesis, transcription initiation, DNA-dependent, transcription termination, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter DNA binding, ATPase activity nucleus, chromosome, nucleolus BY4742 [2-4] G 2 YBR246W RRT2 ERE1 Diphthamide synthetase; required for last step of diphthamide biosynthesis, deletion leads to accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription peptidyl-amino acid modification, endosome transport ligase activity cytoplasm BY4742 [2-4] G 3 - - - - - - - - [2-4] G 4 YBR248C HIS7 - Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, transferring glycosyl groups other BY4742 [2-4] G 5 YBR249C ARO4 - 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups nucleus, cytoplasm BY4742 [2-4] G 6 YBR250W SPO23 - Protein of unknown function; associates with meiosis-specific protein Spo1p meiotic cell cycle molecular_function cellular_component BY4742 [2-4] G 7 YBR251W MRPS5 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [2-4] G 8 - - - - - - - - [2-4] G 9 - - - - - - - - [2-4] G 10 - - - - - - - - [2-4] G 11 YBR255W MTC4 - Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm BY4742 [2-4] G 12 - - - - - - - - [2-4] H 1 - - - - - - - - [2-4] H 2 YBR258C SHG1 CPS15 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus BY4742 [2-4] H 3 YBR259W - - Putative protein of unknown function; YBR259W is not an essential gene biological_process molecular_function cellular_component BY4742 [2-4] H 4 YBR260C RGD1 - GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization response to chemical stimulus, signaling, response to osmotic stress lipid binding, enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-4] H 5 YBR261C TAE1 NTM1 AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm peptidyl-amino acid modification, cytoplasmic translation, protein alkylation methyltransferase activity cytoplasm BY4742 [2-4] H 6 YBR262C AIM5 MCS12|FMP51 Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-4] H 7 YBR263W SHM1 TMP3|SHMT1 Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine cellular amino acid metabolic process other mitochondrion, cytoplasm BY4742 [2-4] H 8 YBR264C YPT10 - Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles other GTPase activity cytoplasm BY4742 [2-4] H 9 - - - - - - - - [2-4] H 10 YBR266C SLM6 - Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase membrane invagination, endocytosis molecular_function cellular_component BY4742 [2-4] H 11 YBR267W REI1 - Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network mitotic cell cycle, cell budding, ribosomal large subunit biogenesis, nuclear transport DNA binding ribosome, cytoplasm BY4742 [2-4] H 12 YBR268W MRPL37 YmL37 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [3-1] A 1 YCL001W RER1 - Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus BY4742 [3-1] A 2 YCL002C - - Putative protein of unknown function; YCL002C is not an essential gene biological_process molecular_function membrane BY4742 [3-1] A 3 YCL003W - - - - - - BY4742 [3-1] A 4 - - - - - - - - [3-1] A 5 YCL005W LDB16 - Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria biological_process molecular_function mitochondrion, cytoplasm BY4742 [3-1] A 6 YCL006C - - - - - - BY4742 [3-1] A 7 YCL007C - CWH36 Dubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A biological_process molecular_function cellular_component BY4742 [3-1] A 8 YCL008C STP22 VPL15|VPS23 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process small conjugating protein binding membrane, plasma membrane, cytoplasm BY4742 [3-1] A 9 YCL009C ILV6 - Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria cellular amino acid metabolic process enzyme regulator activity mitochondrion, cytoplasm BY4742 [3-1] A 10 YCL010C SGF29 - Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA recruitment to target promoters and H3 acetylation peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification protein binding transcription factor activity, histone binding nucleus BY4742 [3-1] A 11 YCL011C GBP2 RLF6 Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro telomere organization, nucleobase-containing compound transport, nuclear transport RNA binding, DNA binding nucleus, cytoplasm BY4742 [3-1] A 12 YCL012W - - - - - - BY4742 [3-1] B 1 YCL013W - - - - - - BY4742 [3-1] B 2 YCL014W BUD3 YCL012W Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding cell budding, cytokinesis molecular_function cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [3-1] B 3 YCL016C DCC1 - Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance response to DNA damage stimulus, mitotic cell cycle, organelle fission, chromosome segregation molecular_function chromosome BY4742 [3-1] B 4 - - - - - - - - [3-1] B 5 - - - - - - - - [3-1] B 6 - - - - - - - - [3-1] B 7 - - - - - - - - [3-1] B 8 - - - - - - - - [3-1] B 9 - - - - - - - - [3-1] B 10 YCL023C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 biological_process molecular_function cellular_component BY4742 [3-1] B 11 YCL024W KCC4 - Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p protein phosphorylation, cell budding, cytoskeleton organization, cytokinesis, protein complex biogenesis, regulation of cell cycle kinase activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [3-1] B 12 YCL025C AGP1 YCC5 Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) transmembrane transport, amino acid transport transmembrane transporter activity membrane, plasma membrane BY4742 [3-1] C 1 YCL026C - - - - - - BY4742 [3-1] C 2 YCL027W FUS1 - Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate cell morphogenesis, conjugation, response to chemical stimulus molecular_function membrane, site of polarized growth, plasma membrane BY4742 [3-1] C 3 YCL028W RNQ1 [PIN(+)] [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate biological_process molecular_function cytoplasm BY4742 [3-1] C 4 YCL029C BIK1 PAC14|ARM5 Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 mitotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, conjugation, regulation of organelle organization, protein complex biogenesis, nucleus organization cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, chromosome, microtubule organizing center, site of polarized growth, cytoplasm BY4742 [3-1] C 5 YCL030C HIS4 - Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis cellular amino acid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, oxidoreductase activity other BY4742 [3-1] C 6 - - - - - - - - [3-1] C 7 YCL032W STE50 - Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction response to heat, conjugation, signaling, response to osmotic stress, response to chemical stimulus enzyme regulator activity cytoplasm BY4742 [3-1] C 8 YCL033C MXR2 MSRB Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm BY4742 [3-1] C 9 YCL034W LSB5 - Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat membrane invagination, cytoskeleton organization, endocytosis molecular_function cell cortex, cytoskeleton, cytoplasm BY4742 [3-1] C 10 - - - - - - - - [3-1] C 11 YCL036W GFD2 YCD6 Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation biological_process molecular_function cellular_component BY4742 [3-1] C 12 YCL037C SRO9 - Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif cytoplasmic translation RNA binding other BY4742 [3-1] D 1 YCL038C ATG22 AUT4 Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation transmembrane transport, amino acid transport molecular_function membrane, vacuole, cytoplasm BY4742 [3-1] D 2 YCL039W GID7 MOH2 Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm BY4742 [3-1] D 3 YCL040W GLK1 HOR3 Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources carbohydrate metabolic process, carbohydrate transport, generation of precursor metabolites and energy kinase activity membrane fraction, cytoplasm BY4742 [3-1] D 4 - - - - - - - - [3-1] D 5 YCL042W - - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [3-1] D 6 - - - - - - - - [3-1] D 7 YCL044C MGR1 - Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [3-1] D 8 YCL045C EMC1 - Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [3-1] D 9 YCL046W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C biological_process molecular_function cellular_component BY4742 [3-1] D 10 YCL047C POF1 - Protein involved in the filamentation pathway; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion invasive growth in response to glucose limitation, pseudohyphal growth molecular_function cellular_component BY4742 [3-1] D 11 YCL048W SPS22 - Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane BY4742 [3-1] D 12 YCL049C - - Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane fraction BY4742 [3-1] E 1 YCL050C APA1 DTP1 Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p nucleobase-containing small molecule metabolic process other nucleus, cytoplasm BY4742 [3-1] E 2 YCL051W LRE1 - Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway cell wall organization or biogenesis molecular_function cellular_component BY4742 [3-1] E 3 - - - - - - - - [3-1] E 4 - - - - - - - - [3-1] E 5 - - - - - - - - [3-1] E 6 YCL055W KAR4 - Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone mitotic cell cycle, meiotic cell cycle, organelle fusion, response to chemical stimulus, conjugation, transcription from RNA polymerase II promoter, nucleus organization nucleic acid binding transcription factor activity nucleus, cytoplasm BY4742 [3-1] E 7 YCL056C PEX34 - Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation peroxisome organization molecular_function peroxisome, membrane, cytoplasm BY4742 [3-1] E 8 YCL057W PRD1 - Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins other peptidase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [3-1] E 9 - - - - - - - - [3-1] E 10 - - - - - - - - [3-1] E 11 YCL060C - - - - - - BY4742 [3-1] E 12 YCL061C MRC1 YCL060C S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres mitotic cell cycle, DNA repair, telomere organization, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [3-1] F 1 YCL062W - - - - - - BY4742 [3-1] F 2 YCL063W VAC17 YCL062W Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p vacuole organization, organelle inheritance other membrane, vacuole, cytoplasm BY4742 [3-1] F 3 YCL064C CHA1 - Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [3-1] F 4 - - - - - - - - [3-1] F 5 - - - - - - - - [3-1] F 6 - - - - - - - - [3-1] F 7 - - - - - - - - [3-1] F 8 YCL069W VBA3 - Permease of basic amino acids in the vacuolar membrane transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [3-1] F 9 - - - - - - - - [3-1] F 10 - - - - - - - - [3-1] F 11 - - - - - - - - [3-1] F 12 - - - - - - - - [3-1] G 1 YCR001W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] G 2 YCR002C CDC10 - Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus contributes septin interaction with phosphatidylinositol-4,5-bisphosphate mitotic cell cycle, sporulation, cytoskeleton organization, cytokinesis, protein complex biogenesis GTPase activity, lipid binding, structural molecule activity cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [3-1] G 3 YCR003W MRPL32 YmL32 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [3-1] G 4 YCR004C YCP4 - Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other membrane, membrane fraction, mitochondrion, cytoplasm BY4742 [3-1] G 5 YCR005C CIT2 - Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors carbohydrate metabolic process, cellular amino acid metabolic process transferase activity, transferring acyl groups peroxisome, mitochondrion, cytoplasm BY4742 [3-1] G 6 YCR006C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [3-1] G 7 YCR007C - - Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] G 8 YCR008W SAT4 HAL4 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p mitotic cell cycle, cellular ion homeostasis, protein dephosphorylation kinase activity cellular_component BY4742 [3-1] G 9 YCR009C RVS161 SPE161|FUS7|END6 Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress cytoskeleton organization, membrane invagination, conjugation, response to starvation, response to osmotic stress, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, membrane, site of polarized growth, cytoplasm BY4742 [3-1] G 10 YCR010C ADY2 ATO1 Acetate transporter required for normal sporulation; phosphorylated in mitochondria transmembrane transport, ion transport transmembrane transporter activity membrane fraction, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [3-1] G 11 YCR011C ADP1 - Putative ATP-dependent permease of the ABC transporter family of proteins transmembrane transport ATPase activity, transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm BY4742 [3-1] G 12 - - - - - - - - [3-1] H 1 - - - - - - - - [3-1] H 2 YCR014C POL4 POLX DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta response to DNA damage stimulus, DNA repair nucleotidyltransferase activity nucleus BY4742 [3-1] H 3 YCR015C - - Putative protein of unknown function; YCR015C is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] H 4 YCR016W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus BY4742 [3-1] H 5 YCR017C CWH43 - Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion lipid metabolic process, cell wall organization or biogenesis, protein lipidation molecular_function cellular bud, membrane, site of polarized growth, plasma membrane BY4742 [3-1] H 6 - - - - - - - - [3-1] H 7 YCR019W MAK32 - Protein necessary for structural stability of L-A double-stranded RNA-containing particles other molecular_function cellular_component BY4742 [3-1] H 8 YCR020C PET18 HIT2 Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin vitamin metabolic process molecular_function cytoplasm BY4742 [3-1] H 9 YCR020C-A MAK31 NAA38|SMX1|LSM9 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family protein acylation transferase activity, transferring acyl groups cytoplasm BY4742 [3-1] H 10 YCR021C HSP30 YRO1 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase nucleobase-containing small molecule metabolic process molecular_function membrane, membrane fraction, plasma membrane BY4742 [3-1] H 11 YCR022C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] H 12 YCR023C - - Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-1] A 1 YDL001W RMD1 - Cytoplasmic protein required for sporulation biological_process molecular_function cytoplasm BY4742 [4-1] A 2 YDL002C NHP10 HMO2 Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair response to DNA damage stimulus, DNA repair, chromatin organization DNA binding nucleus, chromosome BY4742 [4-1] A 3 - - - - - - - - [4-1] A 4 - - - - - - - - [4-1] A 5 YDL005C MED2 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus BY4742 [4-1] A 6 YDL006W PTC1 TPD1|KCS2|CWH47 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation RNA splicing, protein phosphorylation, protein dephosphorylation, mitochondrion organization, response to chemical stimulus, regulation of protein modification process, tRNA processing, conjugation, organelle inheritance, signaling, response to osmotic stress phosphatase activity nucleus, cytoplasm BY4742 [4-1] A 7 - - - - - - - - [4-1] A 8 - - - - - - - - [4-1] A 9 YDL009C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YDL010W; YDL009C is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] A 10 YDL010W GRX6 - Cis-golgi localized monothiol glutaredoxin that binds an iron-sulfur cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; functional overlap with GRX7 response to oxidative stress, response to chemical stimulus oxidoreductase activity, ion binding endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [4-1] A 11 YDL011C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W biological_process molecular_function cellular_component BY4742 [4-1] A 12 YDL012C - - Tail-anchored plasma membrane protein containing a conserved CYSTM module, possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype biological_process molecular_function membrane, plasma membrane BY4742 [4-1] B 1 YDL013W SLX5 ULS2|HEX3 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers response to DNA damage stimulus, telomere organization, protein modification by small protein conjugation or removal small conjugating protein binding, ligase activity nucleus BY4742 [4-1] B 2 - - - - - - - - [4-1] B 3 - - - - - - - - [4-1] B 4 - - - - - - - - [4-1] B 5 - - - - - - - - [4-1] B 6 YDL018C ERP3 - Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport other molecular_function membrane BY4742 [4-1] B 7 YDL019C OSH2 - Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability lipid transport, membrane invagination, exocytosis, endocytosis lipid binding cell cortex, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [4-1] B 8 YDL020C RPN4 UFD5|SON1 Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses DNA repair, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, response to DNA damage stimulus, regulation of DNA metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [4-1] B 9 YDL021W GPM2 - Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process molecular_function, isomerase activity cytoplasm BY4742 [4-1] B 10 YDL022W GPD1 OSR5|OSG1|HOR1|DAR1 NAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to osmotic stress oxidoreductase activity peroxisome, nucleus, cytoplasm BY4742 [4-1] B 11 YDL023C - SRF4 Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component BY4742 [4-1] B 12 YDL024C DIA3 - Protein of unknown function, involved in invasive and pseudohyphal growth invasive growth in response to glucose limitation, pseudohyphal growth phosphatase activity cell wall BY4742 [4-1] C 1 YDL025C RTK1 - Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1 biological_process kinase activity cellular_component BY4742 [4-1] C 2 YDL026W - - Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-1] C 3 YDL027C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene biological_process molecular_function mitochondrion, cytoplasm BY4742 [4-1] C 4 - - - - - - - - [4-1] C 5 - - - - - - - - [4-1] C 6 - - - - - - - - [4-1] C 7 - - - - - - - - [4-1] C 8 YDL032W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] C 9 YDL033C SLM3 MTU1|MTO2 tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) tRNA processing, mitochondrion organization, nucleobase-containing small molecule metabolic process, RNA modification methyltransferase activity mitochondrion, cytoplasm BY4742 [4-1] C 10 YDL034W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] C 11 YDL035C GPR1 - Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis invasive growth in response to glucose limitation, pseudohyphal growth, signaling, response to chemical stimulus signal transducer activity membrane, plasma membrane BY4742 [4-1] C 12 YDL036C PUS9 - Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence tRNA processing, RNA modification isomerase activity nucleus, mitochondrion, cytoplasm BY4742 [4-1] D 1 YDL037C BSC1 - Protein of unconfirmed function, similar to cell surface flocculin Muc1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function cellular_component BY4742 [4-1] D 2 YDL038C - - - - - - BY4742 [4-1] D 3 YDL039C PRM7 YDL038C Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements conjugation molecular_function membrane BY4742 [4-1] D 4 YDL040C NAT1 NAA15|AAA1 Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing protein acylation transferase activity, transferring acyl groups ribosome, mitochondrion, cytoplasm BY4742 [4-1] D 5 YDL041W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C biological_process molecular_function cellular_component BY4742 [4-1] D 6 YDL042C SIR2 MAR1 Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication DNA repair, chromatin organization, DNA replication, response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, chromosome, nucleolus BY4742 [4-1] D 7 - - - - - - - - [4-1] D 8 YDL044C MTF2 NAM1 Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription cytoplasmic translation, mRNA processing RNA binding mitochondrion, cytoplasm BY4742 [4-1] D 9 - - - - - - - - [4-1] D 10 YDL045W-A MRP10 YmS-T Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs mitochondrial translation, mitochondrion organization, protein complex biogenesis structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [4-1] D 11 YDL046W NPC2 - Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes lipid transport molecular_function vacuole, cytoplasm BY4742 [4-1] D 12 - - - - - - - - [4-1] E 1 YDL048C STP4 - Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p biological_process DNA binding nucleus, mitochondrion, cytoplasm BY4742 [4-1] E 2 YDL049C KNH1 - Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance carbohydrate metabolic process molecular_function cell wall, extracellular region BY4742 [4-1] E 3 YDL050C - - Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-1] E 4 YDL051W LHP1 La protein|YLA1|LAH1 RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen tRNA processing RNA binding nucleus, nucleolus BY4742 [4-1] E 5 YDL052C SLC1 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes lipid metabolic process transferase activity, transferring acyl groups cytoplasm BY4742 [4-1] E 6 YDL053C PBP4 - Pbp1p binding protein, interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay biological_process molecular_function nucleus, cytoplasm BY4742 [4-1] E 7 YDL054C MCH1 - Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport other other membrane BY4742 [4-1] E 8 - - - - - - - - [4-1] E 9 YDL056W MBP1 - Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes mitotic cell cycle, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [4-1] E 10 YDL057W - - Putative protein of unknown function; YDL057W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] E 11 - - - - - - - - [4-1] E 12 YDL059C RAD59 - Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p response to DNA damage stimulus, DNA repair, telomere organization, DNA recombination DNA binding nucleus BY4742 [4-1] F 1 - - - - - - - - [4-1] F 2 YDL061W - - - - - - BY4742 [4-1] F 3 YDL062W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential biological_process molecular_function cellular_component BY4742 [4-1] F 4 YDL063C - - Protein required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus ribosomal large subunit biogenesis molecular_function nucleus, cytoplasm BY4742 [4-1] F 5 - - - - - - - - [4-1] F 6 YDL065C PEX19 PAS12 Chaperone and import receptor for newly-synthesized class I peroxisomal membrane proteins (PMPs), binds PMPs in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane protein targeting, peroxisome organization other peroxisome, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-1] F 7 YDL066W IDP1 - Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-1] F 8 YDL067C COX9 - Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-1] F 9 YDL068W - -