mat a Synced to SGD on Wed Feb 1 10:00:08 2012 Plate # Row Column ORF Gene Alias Description GO Biological Process GO Molecular Function GO Cellular Component record no. strain batch 1 A 2 YAL068C PAU8 - Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 338 BY4741 01_1 1 A 3 YAL067C SEO1 - Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide other other membrane 339 BY4741 01_1 1 A 4 YAL066W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 340 BY4741 01_1 1 A 5 YAL065C - - Putative protein of unknown function; has homology to FLO1; possible pseudogene biological_process molecular_function cellular_component 341 BY4741 01_1 1 A 6 YAL062W GDH3 FUN51 NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources cellular amino acid metabolic process oxidoreductase activity nucleus, mitochondrion, cytoplasm 345 BY4741 01_1 1 A 7 YAL061W BDH2 - Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity nucleus, cytoplasm 346 BY4741 01_1 1 A 8 YAL060W BDH1 (2R,3R)-2,3-butanediol dehydrogenase|BDH NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source other oxidoreductase activity cytoplasm 347 BY4741 01_1 1 A 9 YAL059W ECM1 - Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm cell wall organization or biogenesis, ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus 348 BY4741 01_1 1 A 10 YAL058W CNE1 FUN48 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast protein folding, proteolysis involved in cellular protein catabolic process unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 349 BY4741 01_1 1 A 11 YAL056W GPB2 KRH1 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; homolog of Gpb1p invasive growth in response to glucose limitation, sporulation, response to chemical stimulus, pseudohyphal growth, transcription from RNA polymerase II promoter, signaling enzyme regulator activity, signal transducer activity mitochondrion, membrane, cytoplasm, plasma membrane 351 BY4741 01_1 1 A 12 YAL055W PEX22 YAF5 Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation protein targeting, peroxisome organization, signaling other peroxisome, membrane, cytoplasm 352 BY4741 01_1 1 B 1 YAL053W FLC2 HUF2 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance protein folding, transmembrane transport, cell wall organization or biogenesis, nucleobase-containing compound transport transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 354 BY4741 01_1 1 B 2 YAL051W OAF1 YAF1 Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis lipid metabolic process, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 355 BY4741 01_1 1 B 3 YAL049C AIM2 - Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor biological_process molecular_function cytoplasm 356 BY4741 01_1 1 B 4 YAL048C GEM1 GON1 Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases mitochondrion organization, regulation of organelle organization, organelle inheritance GTPase activity, ion binding endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 357 BY4741 01_1 1 B 5 YAL046C AIM1 - Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 359 BY4741 01_1 1 B 6 YAL045C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A biological_process molecular_function cellular_component 360 BY4741 01_1 1 B 7 YAL044C GCV3 - H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 361 BY4741 01_1 1 B 8 YAL042W ERV46 FUN9 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus 363 BY4741 01_1 1 B 9 YAL043C-a - - - - - - 364 BY4741 01_1 1 B 10 YAL040C CLN3 WHI1|FUN10|DAF1 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis vacuole organization, mitotic cell cycle, protein phosphorylation, membrane fusion, regulation of protein modification process, transcription from RNA polymerase II promoter, regulation of cell cycle enzyme regulator activity nucleus 366 BY4741 01_1 1 B 11 YAL039C CYC3 CCHL|cytochrome c heme lyase Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS) protein complex biogenesis lyase activity mitochondrial envelope, mitochondrion, cytoplasm 367 BY4741 01_1 1 B 12 YAL037W - - Putative protein of unknown function biological_process molecular_function cellular_component 369 BY4741 01_1 1 C 1 YAL036C RBG1 FUN11 Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid cytoplasmic translation other ribosome, cytoplasm 370 BY4741 01_1 1 C 2 YAL035W FUN12 eIF5B|yIF2 GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 translational initiation, regulation of translation RNA binding, GTPase activity, translation factor activity, nucleic acid binding ribosome, mitochondrion, cytoplasm 371 BY4741 01_1 1 C 3 YAL034C FUN19 - Non-essential protein of unknown function; expression induced in response to heat stress biological_process molecular_function cellular_component 374 BY4741 01_1 1 C 4 YAL031C GIP4 FUN21 Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate chromosome segregation enzyme binding, enzyme regulator activity cytoplasm 377 BY4741 01_1 1 C 5 YAL030W SNC1 - Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, membrane invagination, exocytosis, endocytosis other endomembrane system, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 378 BY4741 01_1 1 C 6 YAL029C MYO4 SHE1|FUN22 One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p organelle inheritance cytoskeletal protein binding cellular bud, site of polarized growth, cytoskeleton, mitochondrion, cytoplasm 379 BY4741 01_1 1 C 7 YAL028W FRT2 HPH2 Tail-anchored ER membrane protein, interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate other molecular_function endoplasmic reticulum, cytoplasm 380 BY4741 01_1 1 C 8 YAL027W SAW1 - Protein involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus response to DNA damage stimulus, DNA repair, DNA recombination molecular_function nucleus 381 BY4741 01_1 1 C 9 YAL026C DRS2 SWA3|FUN38 Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease organelle assembly, ribosomal small subunit biogenesis, ribosome assembly, Golgi vesicle transport, lipid transport, membrane invagination, endocytosis ATPase activity membrane, Golgi apparatus, cytoplasm 382 BY4741 01_1 1 C 10 YAL023C PMT2 FUN25 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein Ser/Thr residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5; target for new antifungal carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 385 BY4741 01_1 1 C 11 YAL022C FUN26 - Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, cytoplasm 386 BY4741 01_1 1 C 12 YAL021C CCR4 NUT21|FUN27 Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening mitotic cell cycle, DNA replication, RNA catabolic process, transcription elongation, DNA-dependent, mRNA processing, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle nuclease activity nucleus, cytoplasm 387 BY4741 01_1 1 D 1 YAL020C ATS1 KTI13|FUN28 Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle tRNA processing, cell budding, cytoskeleton organization, RNA modification molecular_function cytoplasm 388 BY4741 01_1 1 D 2 YAL019W FUN30 - Conserved member of the Snf2p family with ATP-dependent chromatin remodeling activity; has a role in silencing; potential Cdc28p substrate; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies chromatin organization DNA binding, ATPase activity nucleus, chromosome, mitochondrion, cytoplasm 389 BY4741 01_1 1 D 3 YAL018C - - Putative protein of unknown function biological_process molecular_function membrane 390 BY4741 01_1 1 D 4 YAL017W PSK1 FUN31 One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity cytoplasm 391 BY4741 01_1 1 D 5 YAL015C NTG1 ogg2|SCR1|FUN33 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress DNA repair, DNA replication, mitochondrion organization, regulation of organelle organization, response to oxidative stress, response to chemical stimulus, response to DNA damage stimulus, regulation of DNA metabolic process hydrolase activity, acting on glycosyl bonds, lyase activity nucleus, mitochondrion, cytoplasm 393 BY4741 01_1 1 D 6 YAL014C SYN8 SLT2|UIP2 Endosomal SNARE related to mammalian syntaxin 8 other other cytoplasm 394 BY4741 01_1 1 D 7 YAL013W DEP1 FUN54 Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation response to heat, lipid metabolic process, chromatin organization, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, histone modification molecular_function nucleus, chromosome 395 BY4741 01_1 1 D 8 YAL011W SWC3 SWC1 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae chromatin organization molecular_function nucleus, chromosome, mitochondrion, cytoplasm 397 BY4741 01_1 1 D 9 YAL010C MDM10 FUN37 Subunit of both the ERMES complex that links the ER to mitochondria, and of the mitochondrial sorting and assembly machinery (SAM complex) that functions in import and assembly of outer membrane beta-barrel proteins protein targeting, mitochondrion organization, protein complex biogenesis, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 398 BY4741 01_1 1 D 10 YAL009W SPO7 - Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation meiotic cell cycle, lipid metabolic process, sporulation, nucleus organization phosphatase activity membrane, endomembrane system 399 BY4741 01_1 1 D 11 YAL008W FUN14 - Mitochondrial protein of unknown function biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 400 BY4741 01_1 1 D 12 YAL007C ERP2 - Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 401 BY4741 01_1 1 E 1 YAL004W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C biological_process molecular_function cellular_component 402 BY4741 01_1 1 E 2 YAL005C SSA1 YG100 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall; 98% identical with Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes cytoplasmic translation, protein targeting, mitochondrion organization, transmembrane transport, protein folding, nuclear transport unfolded protein binding, ATPase activity nucleus, membrane fraction, vacuole, cell wall, membrane, cytoplasm 403 BY4741 01_1 1 E 3 YAL002W VPS8 VPL8|VPT8|FUN15 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif protein targeting, endosome transport enzyme binding membrane fraction, cytoplasm 405 BY4741 01_1 1 E 4 YAR002W NUP60 - Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; involved in nuclear export and cytoplasmic localization of specific mRNAs such as ASH1 response to heat, DNA repair, protein targeting, nucleobase-containing compound transport, response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport lipid binding, structural molecule activity nucleus, endomembrane system, membrane 407 BY4741 01_1 1 E 5 YAR003W SWD1 SAF49|CPS50|FUN16 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 408 BY4741 01_1 1 E 6 YAR014C BUD14 - Protein involved in bud-site selection, Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cell morphogenesis, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis enzyme regulator activity cellular bud, site of polarized growth 413 BY4741 01_1 1 E 7 YAR015W ADE1 - N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process ligase activity nucleus, cytoplasm 414 BY4741 01_1 1 E 8 YAR018C KIN3 NPK1|FUN52 Nonessential serine/threonine protein kinase; possible role in DNA damage response chromosome segregation kinase activity cellular_component 415 BY4741 01_1 1 E 9 YAR020C PAU7 - Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component 417 BY4741 01_1 1 E 10 YAR023C - - Putative integral membrane protein, member of DUP240 gene family biological_process molecular_function cellular_component 418 BY4741 01_1 1 E 11 YAR027W UIP3 - Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family biological_process molecular_function nucleus, endomembrane system 419 BY4741 01_1 1 E 12 YAR028W - - Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component 420 BY4741 01_1 1 F 1 YAR029W - - Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm 421 BY4741 01_1 1 F 2 YAR031W PRM9 - Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family vesicle organization molecular_function membrane, endoplasmic reticulum, plasma membrane, cytoplasm 422 BY4741 01_1 1 F 3 YAR030C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 biological_process molecular_function cellular_component 423 BY4741 01_1 1 F 4 YAR035W YAT1 - Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated cellular amino acid metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm 425 BY4741 01_1 1 F 5 YAR037W - - - - - - 426 BY4741 01_1 1 F 6 YAR040C - - - - - - 427 BY4741 01_1 1 F 7 YAR042W SWH1 OSH1|YAR044W Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction lipid transport, membrane invagination, exocytosis, endocytosis lipid binding nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm 428 BY4741 01_1 1 F 8 YAR043C - - - - - - 429 BY4741 01_1 1 F 9 YAR044W - - - - - - 430 BY4741 01_1 1 F 10 YAR047C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 431 BY4741 01_1 1 F 11 YLL001W DNM1 - Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance organelle fission, mitochondrion organization, peroxisome organization, protein complex biogenesis, organelle inheritance GTPase activity peroxisome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1489 BY4741 12_1 1 F 12 YLL002W RTT109 KAT11|REM50|KIM2 Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, transposition, histone modification, response to DNA damage stimulus, transcription from RNA polymerase II promoter transferase activity, transferring acyl groups nucleus 1490 BY4741 12_1 1 G 1 YLL005C SPO75 - Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis sporulation, cell wall organization or biogenesis molecular_function membrane 1493 BY4741 12_1 1 G 2 YLL006W MMM1 YME6 ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth protein targeting, mitochondrion organization, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 1494 BY4741 12_1 1 G 3 YLL009C COX17 - Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrion organization, ion transport, protein complex biogenesis ion binding mitochondrial envelope, mitochondrion, cytoplasm 1497 BY4741 12_1 1 G 4 YLL010C PSR1 - Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p other phosphatase activity membrane, plasma membrane 1498 BY4741 12_1 1 G 5 YLL012W YEH1 - Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes lipid metabolic process other membrane, cytoplasm 1500 BY4741 12_1 1 G 6 YLL013C PUF3 - Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins mitochondrion organization, generation of precursor metabolites and energy, cellular respiration, RNA catabolic process RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 1501 BY4741 12_1 1 G 7 YLL014W EMC6 - Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1502 BY4741 12_1 1 G 8 YLL015W BPT1 - ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p ion transport transmembrane transporter activity membrane, vacuole, cytoplasm 1503 BY4741 12_1 1 G 9 YLL016W - - - - - - 1504 BY4741 12_1 1 G 10 YLL017W - - - - - - 1505 BY4741 12_1 1 G 11 YLL019C KNS1 L124 Nonessential putative protein kinase of unknown cellular role; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues protein phosphorylation kinase activity cellular_component 1507 BY4741 12_1 1 G 12 YLL020C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 biological_process molecular_function cellular_component 1508 BY4741 12_1 1 H 1 YLL021W SPA2 PEA1|FUS6 Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate cell morphogenesis, cell budding, cytoskeleton organization, cytokinesis, response to chemical stimulus, conjugation, pseudohyphal growth, signaling cytoskeletal protein binding cell cortex, cellular bud, site of polarized growth, cytoplasm 1509 BY4741 12_1 1 H 3 YLL023C POM33 - Transmembrane nucleoporin involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p nucleus organization molecular_function nucleus, endomembrane system, ribosome, membrane, endoplasmic reticulum, cytoplasm 1511 BY4741 12_1 1 H 4 YLL024C SSA2 YG102 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes protein targeting, transmembrane transport, protein folding unfolded protein binding, ATPase activity membrane fraction, vacuole, mitochondrion, cell wall, membrane, cytoplasm 1512 BY4741 12_1 1 H 5 YLL025W PAU17 - Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene biological_process molecular_function cellular_component 1513 BY4741 12_1 1 H 6 YLL026W HSP104 - Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation response to heat, carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus, protein folding, oligosaccharide metabolic process unfolded protein binding, ATPase activity nucleus, cytoplasm 1514 BY4741 12_1 1 H 7 YLL028W TPO1 - Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane other transmembrane transporter activity cellular bud, membrane, vacuole, plasma membrane, cytoplasm 1516 BY4741 12_1 1 H 8 YLL029W FRA1 RUP2 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation transcription from RNA polymerase II promoter, response to chemical stimulus molecular_function cytoplasm 1517 BY4741 12_1 1 H 9 YLL032C - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene biological_process molecular_function ribosome, cytoplasm 1520 BY4741 12_1 1 H 10 YLL033W IRC19 RRG4|GON2 Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci sporulation, DNA recombination molecular_function cellular_component 1521 BY4741 12_1 1 H 11 YLL038C ENT4 - Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome membrane invagination, cytoskeleton organization, endocytosis other cell cortex, cytoskeleton, cytoplasm 1526 BY4741 12_1 1 H 12 YLL039C UBI4 UB14|SCD2 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats sporulation, protein modification by small protein conjugation or removal other cytoplasm 1527 BY4741 12_1 2 A 1 YLL040C VPS13 VPT2|SOI1 Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention sporulation, endosome transport molecular_function membrane, mitochondrion, cytoplasm 1528 BY4741 12_1 2 A 3 YLL041C SDH2 SDHB|ACN17 Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1529 BY4741 12_1 2 A 4 YLL042C ATG10 APG10 Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy protein targeting, membrane invagination, response to starvation ligase activity cellular_component 1530 BY4741 12_1 2 A 5 YLL043W FPS1 - Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress ion transport, response to chemical stimulus, conjugation, carbohydrate transport transmembrane transporter activity vacuole, membrane, cytoplasm, plasma membrane 1531 BY4741 12_1 2 A 6 YLL045C RPL8B rp6|YL5|L8B|L4B|SCL41|KRB1 Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1533 BY4741 12_1 2 A 7 YLL046C RNP1 - Ribonucleoprotein that contains two RNA recognition motifs (RRM) other RNA binding cytoplasm 1534 BY4741 12_1 2 A 8 YLL047W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 biological_process molecular_function cellular_component 1535 BY4741 12_1 2 A 9 YLL051C FRE6 - Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels cellular ion homeostasis, transmembrane transport, ion transport oxidoreductase activity membrane, vacuole, cytoplasm 1539 BY4741 12_1 2 A 10 YLL052C AQY2 - Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains other transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 1540 BY4741 12_1 2 A 11 YLL053C - - Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin biological_process molecular_function membrane 1541 BY4741 12_1 2 A 12 YLL054C - - Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene biological_process nucleic acid binding transcription factor activity, DNA binding membrane, plasma membrane 1542 BY4741 12_1 2 B 1 YLL055W YCT1 - High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene amino acid transport transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 1543 BY4741 12_1 2 B 2 YLL056C - - Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component 1544 BY4741 12_1 2 B 3 YLL057C JLP1 - Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation other oxidoreductase activity cellular_component 1545 BY4741 12_1 2 B 4 YLL058W - - Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene other transferase activity, transferring alkyl or aryl (other than methyl) groups cellular_component 1546 BY4741 12_1 2 B 5 YLL060C GTT2 - Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups mitochondrion, cytoplasm 1548 BY4741 12_1 2 B 6 YLL061W MMP1 - High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p amino acid transport transmembrane transporter activity membrane, plasma membrane 1549 BY4741 12_1 2 B 7 YLL062C MHT1 - S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio cellular amino acid metabolic process methyltransferase activity cytoplasm 1550 BY4741 12_1 2 B 8 YLL063C AYT1 - Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis other transferase activity, transferring acyl groups cellular_component 1551 BY4741 12_1 2 B 9 YLR001C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm 1556 BY4741 12_1 2 B 10 YLR003C CMS1 - Subunit of U3-containing 90S preribosome processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; overexpression rescues supressor mutant of mcm10; null mutant is viable ribosomal small subunit biogenesis molecular_function nucleus 1558 BY4741 12_1 2 B 11 YLR004C THI73 - Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs other other membrane, endoplasmic reticulum, cytoplasm 1559 BY4741 12_1 2 B 12 YLR011W LOT6 - FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions protein targeting, transmembrane transport, regulation of transport, response to oxidative stress, response to chemical stimulus, nuclear transport oxidoreductase activity, transcription factor binding nucleus, cytoplasm 1566 BY4741 12_1 2 C 1 YLR012C - - Putative protein of unknown function; YLR012C is not an essential gene biological_process molecular_function cellular_component 1567 BY4741 12_1 2 C 2 YLR013W GAT3 - Protein containing GATA family zinc finger motifs other nucleic acid binding transcription factor activity, DNA binding nucleus 1568 BY4741 12_1 2 C 3 YLR014C PPR1 - Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p nucleobase-containing small molecule metabolic process nucleic acid binding transcription factor activity nucleus 1569 BY4741 12_1 2 C 4 YLR015W BRE2 CPS60 Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L peptidyl-amino acid modification, telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 1570 BY4741 12_1 2 C 5 YLR016C PML1 - Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p RNA splicing, mRNA processing, nucleobase-containing compound transport, nuclear transport molecular_function nucleus, cytoplasm 1571 BY4741 12_1 2 C 6 YLR017W MEU1 - Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression cellular amino acid metabolic process transferase activity, transferring glycosyl groups, RNA binding, mRNA binding cytoplasm 1572 BY4741 12_1 2 C 7 YLR018C POM34 - Integral membrane protein of the nuclear pore; has an important role in maintaining the architecture of the pore complex protein targeting, cytoskeleton organization, nuclear transport, nucleus organization molecular_function nucleus, endomembrane system, membrane 1573 BY4741 12_1 2 C 8 YLR019W PSR2 - Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p other phosphatase activity membrane, plasma membrane 1574 BY4741 12_1 2 C 9 YLR020C YEH2 - Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism lipid metabolic process, cell wall organization or biogenesis other membrane, plasma membrane 1575 BY4741 12_1 2 C 10 YLR021W IRC25 DMP2|PBA3|POC3 Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions RNA catabolic process, protein complex biogenesis molecular_function other 1576 BY4741 12_1 2 C 11 YLR023C IZH3 - Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity cellular ion homeostasis ion binding membrane, endoplasmic reticulum, cytoplasm 1578 BY4741 12_1 2 C 12 YLR024C UBR2 - Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex protein modification by small protein conjugation or removal ligase activity cytoplasm 1579 BY4741 12_1 Incorrect 2 D 1 YLR025W SNF7 VPL5|RNS4|VPS32|DID1 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes endosome transport, vesicle organization, proteolysis involved in cellular protein catabolic process, response to chemical stimulus other membrane, cytoplasm 1580 BY4741 12_1 2 D 2 YLR027C AAT2 ASP5 Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells cellular amino acid metabolic process other peroxisome, cytoplasm 1582 BY4741 12_1 2 D 3 YLR028C ADE16 - Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine sporulation, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm 1583 BY4741 12_1 2 D 4 YLR042C - - Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene biological_process molecular_function cell wall 2653 BY4741 12_2 2 D 5 YLR043C TRX1 LMA1 Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance vacuole organization, DNA replication, response to oxidative stress, response to chemical stimulus, Golgi vesicle transport, membrane fusion, organelle inheritance, regulation of DNA metabolic process oxidoreductase activity vacuole, cytoplasm 2654 BY4741 12_2 2 D 6 YLR044C PDC1 - Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism carbohydrate metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process lyase activity nucleus, cytoplasm 2655 BY4741 12_2 2 D 7 YLR046C - RLA1 Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function membrane 2657 BY4741 12_2 2 D 8 YLR047C FRE8 - Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p cellular ion homeostasis oxidoreductase activity membrane 2658 BY4741 12_2 2 D 9 YLR048W RPS0B S0B|YST2|NAB1B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal cytoplasmic translation, rRNA processing, organelle assembly, nucleobase-containing compound transport, ribosomal small subunit biogenesis, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 2659 BY4741 12_2 2 D 10 YLR049C - - Putative protein of unknown function biological_process molecular_function cellular_component 2660 BY4741 12_2 2 D 11 YLR053C - - Putative protein of unknown function biological_process molecular_function cellular_component 2664 BY4741 12_2 2 D 12 YLR054C OSW2 - Protein of unknown function proposed to be involved in the assembly of the spore wall sporulation, cell wall organization or biogenesis molecular_function membrane, cytoplasm 2665 BY4741 12_2 2 E 1 YLR055C SPT8 - Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 2666 BY4741 12_2 2 E 2 YLR056W ERG3 PSO6|SYR1 C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm 2667 BY4741 12_2 2 E 3 YLR057W MNL2 - Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene proteolysis involved in cellular protein catabolic process molecular_function endoplasmic reticulum, cytoplasm 2668 BY4741 12_2 2 E 4 YLR058C SHM2 SHMT2 Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis other other site of polarized growth, membrane fraction, cytoplasm 2669 BY4741 12_2 2 E 5 YLR059C REX2 YNT20 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease rRNA processing, mitochondrion organization nuclease activity mitochondrion, cytoplasm 2670 BY4741 12_2 2 E 6 YLR061W RPL22A rp4|l1c|YL31|L22A Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2672 BY4741 12_2 2 E 7 YLR062C BUD28 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay cytokinesis molecular_function cellular_component 2673 BY4741 12_2 2 E 8 YLR063W - - Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene biological_process methyltransferase activity cytoplasm 2674 BY4741 12_2 2 E 9 YLR064W PER33 - Protein that localizes to the endoplasmic reticulum with some nuclear pore complex association; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p biological_process molecular_function nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2675 BY4741 12_2 2 E 10 YLR065C ENV10 - Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene protein maturation molecular_function membrane 2676 BY4741 12_2 2 E 11 YLR067C PET309 - Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) translational initiation, mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration other mitochondrion, membrane, mitochondrial envelope, cytoplasm 2678 BY4741 12_2 2 E 12 YLR068W FYV7 - Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 2679 BY4741 12_2 2 F 1 YLR069C MEF1 - Mitochondrial elongation factor involved in translational elongation mitochondrial translation, mitochondrion organization, translational elongation RNA binding, translation factor activity, nucleic acid binding mitochondrion, cytoplasm 2680 BY4741 12_2 2 F 2 YLR070C XYL2 - Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect carbohydrate metabolic process oxidoreductase activity cellular_component 2681 BY4741 12_2 2 F 3 YLR072W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene biological_process molecular_function cytoplasm 2683 BY4741 12_2 2 F 4 YLR073C RFU1 - Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes other enzyme regulator activity cytoplasm 2684 BY4741 12_2 2 F 5 YLR074C BUD20 - Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cytokinesis molecular_function nucleus 2685 BY4741 12_2 2 F 6 YLR077W FMP25 BCA1 Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2688 BY4741 12_2 2 F 7 YLR079W SIC1 SDB25 Inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 mitotic cell cycle, regulation of protein modification process, protein phosphorylation, response to starvation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 2690 BY4741 12_2 2 F 8 YLR080W EMP46 - Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 2691 BY4741 12_2 2 F 9 YLR081W GAL2 - Galactose permease, required for utilization of galactose; also able to transport glucose carbohydrate metabolic process, carbohydrate transport transmembrane transporter activity membrane, plasma membrane 2692 BY4741 12_2 2 F 10 YLR082C SRL2 - Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation other molecular_function nucleus, cytoplasm 2693 BY4741 12_2 2 F 11 YLR083C EMP70 TMN1|p24a Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments cellular ion homeostasis, invasive growth in response to glucose limitation, endosome transport, pseudohyphal growth molecular_function membrane, vacuole, cytoplasm 2694 BY4741 12_2 2 F 12 YLR084C RAX2 - N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p cytokinesis molecular_function cellular bud, cell wall, membrane, site of polarized growth, mitochondrion, cytoplasm 2695 BY4741 12_2 2 G 1 YLR085C ARP6 - Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin organization other nucleus, chromosome, cytoplasm 2696 BY4741 12_2 2 G 2 YLR087C CSF1 - Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies generation of precursor metabolites and energy molecular_function mitochondrion, cytoplasm 2698 BY4741 12_2 2 G 3 YLR089C ALT1 - Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cellular amino acid metabolic process other mitochondrion, cytoplasm 2700 BY4741 12_2 2 G 4 YLR090W XDJ1 - Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein folding other nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm 2701 BY4741 12_2 2 G 5 YLR091W GEP5 RRG5 Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine mitochondrion organization molecular_function mitochondrion, cytoplasm 2702 BY4741 12_2 2 G 6 YLR092W SUL2 - High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates ion transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 2703 BY4741 12_2 2 G 7 YLR093C NYV1 MAM2 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane vacuole organization, organelle fusion, vesicle organization, membrane fusion other vacuole, membrane, cytoplasm 2704 BY4741 12_2 2 G 8 YLR094C GIS3 - Protein of unknown function signaling molecular_function nucleus, cytoplasm 2705 BY4741 12_2 2 G 9 YLR095C IOC2 - Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif chromatin organization DNA binding, ATPase activity nucleus 2706 BY4741 12_2 2 G 10 YLR096W KIN2 - Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p protein phosphorylation, exocytosis kinase activity membrane, plasma membrane 2707 BY4741 12_2 2 G 11 YLR097C HRT3 - Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies proteolysis involved in cellular protein catabolic process, response to chemical stimulus protein binding, bridging, ligase activity other 2708 BY4741 12_2 2 G 12 YLR098C CHA4 SIL3|SIL2 DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 2709 BY4741 12_2 2 H 1 YLR099C ICT1 - Lysophosphatidic acid acyltransferase, responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress lipid metabolic process transferase activity, transferring acyl groups cellular_component 2710 BY4741 12_2 2 H 3 YLR102C APC9 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition chromatin organization, protein modification by small protein conjugation or removal ligase activity nucleus 2713 BY4741 12_2 2 H 4 YLR104W LCL2 - Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) proteolysis involved in cellular protein catabolic process molecular_function cellular_component 2715 BY4741 12_2 2 H 5 YLR107W REX3 - RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases rRNA processing nuclease activity nucleus, cytoplasm 2718 BY4741 12_2 2 H 6 YLR108C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene biological_process molecular_function nucleus 2719 BY4741 12_2 2 H 7 YLR109W AHP1 cTPxIII Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p response to oxidative stress, response to chemical stimulus oxidoreductase activity membrane fraction, cytoplasm 2720 BY4741 12_2 2 H 8 YLR111W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2722 BY4741 12_2 2 H 9 YLR112W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2723 BY4741 12_2 2 H 10 YLR113W HOG1 SSK3 Mitogen-activated protein kinase involved in osmoregulation; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p response to heat, protein phosphorylation, response to chemical stimulus, transcription from RNA polymerase II promoter, signaling, response to osmotic stress kinase activity, signal transducer activity nucleus, cytoplasm 2724 BY4741 12_2 2 H 11 YLR114C AVL9 - Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms Golgi vesicle transport molecular_function cytoplasm 2725 BY4741 12_2 2 H 12 YLR118C - APT1 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS other other nucleus, cytoplasm 2729 BY4741 12_2 3 A 1 YLR119W SRN2 VPL16|VPS37|SRN10 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus protein targeting, proteolysis involved in cellular protein catabolic process other membrane, cytoplasm 2730 BY4741 12_2 3 A 2 YLR120C YPS1 YAP3 Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor cell wall organization or biogenesis, protein maturation peptidase activity cell wall 2731 BY4741 12_2 3 A 4 YLR121C YPS3 YPS4 Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor cell wall organization or biogenesis peptidase activity membrane, plasma membrane 2732 BY4741 12_2 3 A 5 YLR122C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C biological_process molecular_function cellular_component 2733 BY4741 12_2 3 A 6 YLR123C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif biological_process molecular_function cellular_component 2734 BY4741 12_2 3 A 7 YLR124W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2735 BY4741 12_2 3 A 8 YLR125W - - Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene biological_process molecular_function cellular_component 2736 BY4741 12_2 Incorrect 3 A 9 YML089C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage biological_process molecular_function cellular_component 481 BY4730 13_1 3 A 10 YML088W UFO1 - F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, response to chemical stimulus protein binding, bridging, ligase activity nucleus, cytoplasm 482 BY4730 13_1 3 A 11 YML087C AIM33 - Putative protein of unknown function, highly conserved across species and orthologous to human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss biological_process molecular_function cellular_component 483 BY4730 13_1 3 A 12 YML086C ALO1 - D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 484 BY4730 13_1 3 B 1 YML084W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 486 BY4730 13_1 3 B 2 YML083C - - Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component 487 BY4730 13_1 3 B 3 YML082W - - Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene other lyase activity nucleus, cytoplasm 488 BY4730 13_1 3 B 4 YML081W TDA9 - DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process DNA binding nucleus 489 BY4730 13_1 3 B 5 YML080W DUS1 - Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 tRNA processing, RNA modification oxidoreductase activity nucleus 490 BY4730 13_1 3 B 6 YML079W - - Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm biological_process molecular_function nucleus, cytoplasm 491 BY4730 13_1 3 B 7 YML078W CPR3 CYP3 Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria protein folding isomerase activity mitochondrion, cytoplasm 492 BY4730 13_1 3 B 8 YML063W RPS1B rp10B|S1B|RP10B|PLC2 Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 507 BY4741 13_1 3 B 9 YML062C MFT1 MFT52 Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance DNA recombination, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, nucleobase-containing compound transport, nuclear transport structural molecule activity nucleus 508 BY4741 13_1 3 B 10 YML061C PIF1 TST1 DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis telomere organization, DNA replication, mitochondrion organization, regulation of organelle organization, DNA recombination, regulation of DNA metabolic process enzyme regulator activity, helicase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 509 BY4741 13_1 3 B 11 YML060W OGG1 - Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance response to DNA damage stimulus, DNA repair lyase activity, hydrolase activity, acting on glycosyl bonds mitochondrion, cytoplasm 510 BY4741 13_1 3 B 12 YML059C NTE1 - Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes lipid metabolic process other membrane, endoplasmic reticulum, cytoplasm 511 BY4741 13_1 3 C 1 YML058W SML1 - Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase response to DNA damage stimulus, mitochondrion organization enzyme regulator activity nucleus, cytoplasm 512 BY4741 13_1 3 C 2 YML057W CMP2 CNA2 Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 cellular ion homeostasis, conjugation, signaling, response to chemical stimulus phosphatase activity cytoplasm 513 BY4741 13_1 3 C 3 YML058C-A - - - - - - 514 BY4741 13_1 3 C 4 YML056C IMD4 - Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed biological_process oxidoreductase activity cytoplasm 515 BY4741 13_1 3 C 5 YML055W SPC2 SPY1 Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 protein targeting, protein maturation peptidase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 516 BY4741 13_1 3 C 6 YML054C CYB2 - Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions other oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm 517 BY4741 13_1 3 C 7 YML053C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus, cytoplasm 518 BY4741 13_1 3 C 8 YML052W SUR7 - Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants sporulation, membrane invagination, endocytosis molecular_function cell cortex, membrane fraction, mitochondrion, cell wall, membrane, cytoplasm, plasma membrane 519 BY4741 13_1 3 C 9 YML051W GAL80 - Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding carbohydrate metabolic process, transcription from RNA polymerase II promoter transcription factor binding, enzyme regulator activity nucleus, cytoplasm 520 BY4741 13_1 3 C 10 YML050W AIM32 - Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 521 BY4741 13_1 3 C 11 YML048W GSF2 ECM6 ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression protein folding unfolded protein binding membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 523 BY4741 13_1 3 C 12 YML048W-A - - - - - - 524 BY4741 13_1 3 D 1 YML037C - - Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene biological_process molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 534 BY4741 13_2 3 D 2 YML035C AMD1 AMD3 AMP deaminase, tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; may be involved in regulation of intracellular adenine nucleotide pools nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm 536 BY4741 13_2 3 D 3 YML034W SRC1 HEH1|YML033W Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance mitotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, endomembrane system 537 BY4741 13_2 3 D 4 YML035C-A - - - - - - 538 BY4741 13_2 3 D 5 YML033W - - - - - - 539 BY4741 13_2 3 D 6 YML032C RAD52 - Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis meiotic cell cycle, DNA repair, telomere organization, response to DNA damage stimulus, DNA recombination DNA binding nucleus, chromosome 540 BY4741 13_2 3 D 7 YML030W AIM31 - Putative protein of unknown function; GFP-fusion protein localizes to mitochondria; may interact with respiratory chain complexes III or IV; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 543 BY4741 13_2 3 D 8 YML029W USA1 - Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p RNA splicing, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, mRNA processing structural molecule activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 544 BY4741 13_2 3 D 9 YML028W TSA1 cTPxI|TPX1|ZRG14 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype regulation of translation, response to oxidative stress, response to chemical stimulus, response to DNA damage stimulus, protein folding, regulation of cell cycle oxidoreductase activity, unfolded protein binding cytoplasm 545 BY4741 13_2 3 D 10 YML026C RPS18B S18B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins cytoplasmic translation, rRNA processing, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 547 BY4741 13_2 3 D 11 YML024W RPS17A rp51A|S17A|RPL51A|RP51A Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein cytoplasmic translation, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 549 BY4741 13_2 3 D 12 YML020W - - Putative protein of unknown function biological_process molecular_function cellular_component 553 BY4741 13_2 3 E 1 YML019W OST6 - Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p peptidyl-amino acid modification, carbohydrate metabolic process, protein complex biogenesis, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 554 BY4741 13_2 3 E 2 YML018C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 555 BY4741 13_2 3 E 3 YML017W PSP2 MRS15 Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing biological_process molecular_function cytoplasm 556 BY4741 13_2 3 E 4 YML016C PPZ1 - Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis, protein dephosphorylation phosphatase activity nucleus, membrane, plasma membrane, cytoplasm 557 BY4741 13_2 3 E 5 YML014W TRM9 KTI1 tRNA methyltransferase, catalyzes esterification of modified uridine nucleotides in tRNA(Arg3) and tRNA(Glu), likely as part of a complex with Trm112p; deletion confers resistance to zymocin tRNA processing, RNA modification methyltransferase activity nucleus, cytoplasm 559 BY4741 13_2 3 E 6 YML013W UBX2 SEL1 Protein involved in ER-associated protein degradation; proposed to coordinate the assembly of proteins involved in ERAD; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain proteolysis involved in cellular protein catabolic process protein binding, bridging endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 560 BY4741 13_2 3 E 7 YML013C-A - - - - - - 561 BY4741 13_2 3 E 8 YML012W ERV25 - Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 562 BY4741 13_2 3 E 9 YML011C RAD33 - Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus response to DNA damage stimulus, DNA repair molecular_function nucleus 563 BY4741 13_2 3 E 10 YML009c - - - - - - 567 BY4741 13_2 3 E 11 YML008C ERG6 VID1|SED6|LIS1|ISE1 Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to both lipid particles and mitochondrial outer membrane lipid metabolic process methyltransferase activity membrane, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 568 BY4741 13_2 3 E 12 YML007W YAP1 SNQ3|PAR1 Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; mediates resistance to cadmium response to heat, transcription from RNA polymerase II promoter, response to oxidative stress, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 569 BY4741 13_2 3 F 1 YML006C GIS4 - CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway cellular ion homeostasis, signaling molecular_function membrane, plasma membrane 570 BY4741 13_2 3 F 2 YML005W TRM12 TYW2 S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA tRNA processing, nucleobase-containing small molecule metabolic process, RNA modification methyltransferase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 571 BY4741 13_2 3 F 3 YML004C GLO1 - Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress cellular amino acid metabolic process lyase activity nucleus, cytoplasm 572 BY4741 13_2 3 F 4 YML003W - - Putative protein of unknown function biological_process molecular_function cellular_component 573 BY4741 13_2 3 F 5 YML002W - - Putative protein of unknown function; expression induced by heat and by calcium shortage biological_process molecular_function cellular_component 574 BY4741 13_2 3 F 6 YML001W YPT7 VAM4|AST4 Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion, similar to mammalian Rab7 vacuole organization, regulation of organelle organization, membrane fusion, Golgi vesicle transport, membrane invagination, organelle inheritance, endocytosis GTPase activity vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm 575 BY4741 13_2 3 F 7 YMR002W MIC17 - Mitochondrial intermembrane space protein, required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 577 BY4741 13_2 3 F 8 YMR003W AIM34 - Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 578 BY4741 13_2 3 F 9 YMR006C PLB2 - Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine lipid metabolic process other cell wall, extracellular region 581 BY4741 13_2 3 F 10 YMR007W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 582 BY4741 13_2 3 F 11 YMR008C PLB1 - Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol lipid metabolic process other cell wall, membrane fraction 583 BY4741 13_2 3 F 12 YMR009W ADI1 - Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 584 BY4741 13_2 3 G 1 YMR010W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 biological_process molecular_function membrane, cytoplasm 585 BY4741 13_2 3 G 2 YMR011W HXT2 - High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 586 BY4741 13_2 3 G 3 YMR012W CLU1 TIF31 eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant translational initiation, mitochondrion organization molecular_function cytoplasm 587 BY4741 13_2 3 G 4 YMR014W BUD22 - Protein required for 18S rRNA maturation and small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 589 BY4741 13_2 3 G 5 YMR015C ERG5 CYP61 C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs lipid metabolic process oxidoreductase activity endoplasmic reticulum, cytoplasm 590 BY4741 13_2 3 G 6 YMR016C SOK2 - Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors pseudohyphal growth nucleic acid binding transcription factor activity, DNA binding nucleus 591 BY4741 13_2 3 G 7 YMR017W SPO20 DBI9 Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog sporulation other membrane 592 BY4741 13_2 3 G 8 YMR018W - - Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene biological_process molecular_function cellular_component 593 BY4741 13_2 3 G 9 YMR019W STB4 - Protein that binds Sin3p in a two-hybrid assay; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters biological_process DNA binding nucleus 594 BY4741 13_2 3 G 10 YMR020W FMS1 - Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process oxidoreductase activity cytoplasm 595 BY4741 13_2 3 G 11 YMR021C MAC1 CUA1 Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport cellular ion homeostasis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 596 BY4741 13_2 3 G 12 YMR022W UBC7 DER2|QRI8 Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly chromatin organization, proteolysis involved in cellular protein catabolic process, cell wall organization or biogenesis ligase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 597 BY4741 13_2 3 H 1 YMR023C MSS1 PET53 Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 mitochondrion organization, tRNA processing, nucleobase-containing small molecule metabolic process, RNA modification other mitochondrion, membrane, mitochondrial envelope, cytoplasm 598 BY4741 13_2 3 H 3 YMR024W MRPL3 YmL3 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 599 BY4741 13_2 3 H 4 YMR025W CSI1 - Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling protein modification by small protein conjugation or removal, conjugation, signaling, response to chemical stimulus molecular_function nucleus 600 BY4741 13_2 3 H 5 YMR026C PEX12 PAS11 C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder protein targeting, peroxisome organization ligase activity peroxisome, membrane, cytoplasm 601 BY4741 13_2 3 H 6 YMR027W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene biological_process molecular_function nucleus, cytoplasm 602 BY4741 13_2 3 H 7 YMR029C FAR8 - Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p conjugation, signaling, response to chemical stimulus molecular_function cellular_component 604 BY4741 13_2 3 H 8 YMR030W RSF1 - Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes mitochondrion organization, generation of precursor metabolites and energy, cellular respiration, transcription from RNA polymerase II promoter, signaling molecular_function nucleus, mitochondrion, cytoplasm 605 BY4741 13_2 3 H 9 YMR031W-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C biological_process molecular_function cellular_component 606 BY4741 13_2 3 H 10 YMR031C EIS1 - Component of the eisosome that is required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies protein complex biogenesis molecular_function membrane, cell cortex, mitochondrion, cytoplasm 607 BY4741 13_2 3 H 11 YMR032W HOF1 CYK2 Bud neck-localized, SH3 domain-containing protein required for cytokinesis; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p cytokinesis, response to DNA damage stimulus cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 608 BY4741 13_2 3 H 12 YMR034C - - Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene biological_process other membrane 610 BY4741 13_2 4 A 1 YMR035W IMP2 - Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p protein targeting, mitochondrion organization, protein maturation peptidase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 611 BY4741 13_2 4 A 2 YMR036C MIH1 - Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 mitotic cell cycle, regulation of protein modification process, protein phosphorylation, regulation of cell cycle phosphatase activity nucleus, cytoplasm 612 BY4741 13_2 4 A 3 YMR039C SUB1 TSP1 Transcriptional coactivator, facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes transcription from RNA polymerase III promoter, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter, response to osmotic stress protein binding transcription factor activity nucleus 615 BY4741 13_2 4 A 5 YMR040W YET2 - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function membrane, ribosome, cytoplasm 616 BY4741 13_2 4 A 6 YMR041C ARA2 - NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase other oxidoreductase activity cellular_component 617 BY4741 13_2 4 A 7 YMR042W ARG80 ARGRI|ARGR1 Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p transcription from RNA polymerase II promoter, cellular amino acid metabolic process DNA binding nucleus 618 BY4741 13_2 4 A 8 YMR044W IOC4 - Member of a complex (Isw1b) with Isw1p and Ioc2p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PWWP motif chromatin organization DNA binding, ATPase activity nucleus 620 BY4741 13_2 4 A 9 YMR140W SIP5 - Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase response to starvation molecular_function cytoplasm 721 BY4741 13_4 4 A 10 YMR141C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 722 BY4741 13_4 4 A 11 YMR143W RPS16A rp61R|S16A Protein component of the small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosomal proteins cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 724 BY4741 13_4 4 A 12 YMR144W - - Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene biological_process molecular_function nucleus 725 BY4741 13_4 4 B 1 YMR145C NDE1 NDH1 Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy oxidoreductase activity mitochondrion, cytoplasm 726 BY4741 13_4 4 B 2 YMR147W - - Putative protein of unknown function biological_process molecular_function cellular_component 728 BY4741 13_4 4 B 3 YMR148W OSW5 - Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion biological_process molecular_function membrane 729 BY4741 13_4 4 B 4 YMR151W YIM2 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 biological_process molecular_function cellular_component 731 BY4741 13_4 4 B 5 YMR150C IMP1 PET-TS2858 Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p protein targeting, mitochondrion organization, protein maturation peptidase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 732 BY4741 13_4 4 B 6 YMR152W YIM1 - Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies response to DNA damage stimulus molecular_function mitochondrion, cytoplasm 733 BY4741 13_4 4 B 7 YMR153W NUP53 - Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex mitotic cell cycle, protein targeting, organelle fission, regulation of organelle organization, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport, regulation of cell cycle, nucleus organization lipid binding, DNA binding, structural molecule activity nucleus, endomembrane system, membrane 734 BY4741 13_4 4 B 8 YMR153C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 biological_process molecular_function cellular_component 735 BY4741 13_4 4 B 9 YMR155W - - Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen biological_process molecular_function membrane 737 BY4741 13_4 4 B 10 YMR156C TPP1 - DNA 3'-phosphatase that functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; has similarity to the l-2-haloacid dehalogenase superfamily response to DNA damage stimulus, DNA repair phosphatase activity cellular_component 738 BY4741 13_4 4 B 11 YMR157C AIM36 FMP39 Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 739 BY4741 13_4 4 B 12 YMR158W-A - - - - - - 741 BY4741 13_4 4 C 1 YMR159C ATG16 APG15|APG16|CVT11|SAP18 Conserved protein that interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy protein targeting, membrane invagination, response to starvation, protein lipidation protein binding, bridging, ligase activity membrane fraction, cytoplasm 742 BY4741 13_4 4 C 2 YMR161W HLJ1 - Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ proteolysis involved in cellular protein catabolic process enzyme regulator activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 744 BY4741 13_4 4 C 3 YMR162C DNF3 - Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase lipid transport ATPase activity membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 745 BY4741 13_4 4 C 4 YMR163C INP2 - Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene organelle inheritance, peroxisome organization other peroxisome, membrane, cytoplasm 746 BY4741 13_4 4 C 5 YMR164C MSS11 - Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of MUC1 and STA2 in response to nutritional signals carbohydrate metabolic process, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 747 BY4741 13_4 4 C 6 YMR166C - - Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene other other membrane, mitochondrial envelope, mitochondrion, cytoplasm 749 BY4741 13_4 4 C 7 YMR167W MLH1 PMS2 Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity nucleus, mitochondrion, cytoplasm 750 BY4741 13_4 4 C 8 YMR169C ALD3 - Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose cellular amino acid metabolic process oxidoreductase activity cytoplasm 752 BY4741 13_4 4 C 9 YMR170C ALD2 - Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p cellular amino acid metabolic process oxidoreductase activity cytoplasm 753 BY4741 13_4 4 C 10 YMR172C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 756 BY4741 13_4 4 C 11 YMR173W-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W biological_process molecular_function membrane 758 BY4741 13_4 4 C 12 YMR174C PAI3 IA3 Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact other enzyme regulator activity cytoplasm 759 BY4741 13_4 4 D 1 YMR175W SIP18 - Phospholipid-binding hydrophilin with a role in dessication resistance; expression is induced by osmotic stress response to chemical stimulus lipid binding cytoplasm 760 BY4741 13_4 4 D 2 YMR176W ECM5 - Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks cell wall organization or biogenesis molecular_function nucleus, chromosome 761 BY4741 13_4 4 D 3 YMR177W MMT1 - Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p cellular ion homeostasis molecular_function membrane, mitochondrion, cytoplasm 762 BY4741 13_4 4 D 4 YMR178W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR178W is not an essential gene biological_process molecular_function nucleus, cytoplasm 763 BY4741 13_4 4 D 5 YMR179W SPT21 - Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p transcription from RNA polymerase II promoter molecular_function nucleus 764 BY4741 13_4 4 D 6 YMR180C CTL1 CTH1 RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm biological_process phosphatase activity nucleus, cytoplasm 765 BY4741 13_4 4 D 7 YMR182C RGM1 - Putative transcriptional repressor with proline-rich zinc fingers; overproduction impairs cell growth transcription from RNA polymerase II promoter DNA binding nucleus 767 BY4741 13_4 4 D 8 YMR183C SSO2 - Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p sporulation, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion lipid binding membrane fraction, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 768 BY4741 13_4 4 D 9 YMR184W ADD37 - Protein of unknown function involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene proteolysis involved in cellular protein catabolic process molecular_function cytoplasm 769 BY4741 13_4 4 D 10 YMR186W HSC82 HSP90 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock telomere organization, protein folding, protein complex biogenesis unfolded protein binding, ATPase activity membrane fraction, mitochondrion, cytoplasm 771 BY4741 13_4 4 D 11 YMR187C - - Putative protein of unknown function; YMR187C is not an essential gene biological_process molecular_function membrane 772 BY4741 13_4 4 D 12 YMR188C MRPS17 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 773 BY4741 13_4 4 E 1 YMR189W GCV2 GSD2 P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 774 BY4741 13_4 4 E 2 YMR190C SGS1 - Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes mitotic cell cycle, meiotic cell cycle, organelle fission, DNA replication, response to DNA damage stimulus, DNA recombination, DNA repair, telomere organization, chromosome segregation, regulation of DNA metabolic process, regulation of cell cycle helicase activity, ATPase activity nucleus, chromosome, nucleolus 775 BY4741 13_4 4 E 3 YMR191W SPG5 - Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component 776 BY4741 13_4 4 E 4 YMR192W GYL1 APP2 Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth regulation of transport, Golgi vesicle transport, exocytosis other mitochondrion, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 777 BY4741 13_4 4 E 5 YMR193W MRPL24 YmL14|YmL24|MRPL14 Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 778 BY4741 13_4 4 E 6 YMR194W RPL36A YL39|L39|L36A|RPL39B N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Bp and has similarity to rat L36 ribosomal protein; binds to 5.8 S rRNA cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 779 BY4741 13_4 4 E 7 YMR193C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 780 BY4741 13_4 4 E 8 YMR195W ICY1 - Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation biological_process molecular_function membrane, vacuole, cytoplasm 781 BY4741 13_4 4 E 9 YMR196W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene biological_process molecular_function cytoplasm 782 BY4741 13_4 4 E 10 YMR198W CIK1 - Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, chromosome segregation, conjugation, nucleus organization other cytoskeleton, microtubule organizing center, cytoplasm 784 BY4741 13_4 4 E 11 YMR199W CLN1 - G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) regulation of protein modification process, protein phosphorylation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 785 BY4741 13_4 4 E 12 YMR201C RAD14 - Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein response to DNA damage stimulus, DNA repair DNA binding, ion binding nucleus 787 BY4741 13_4 4 F 1 YMR202W ERG2 END11 C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis lipid metabolic process isomerase activity endoplasmic reticulum, cytoplasm 788 BY4741 13_4 4 F 2 YMR204C INP1 - Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane organelle inheritance, peroxisome organization molecular_function peroxisome, membrane, cytoplasm 790 BY4741 13_4 4 F 3 YMR205C PFK2 - Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes carbohydrate metabolic process, generation of precursor metabolites and energy RNA binding, kinase activity, mRNA binding mitochondrion, cytoplasm 791 BY4741 13_4 4 F 4 YMR206W - - Putative protein of unknown function; YMR206W is not an essential gene biological_process molecular_function cellular_component 792 BY4741 13_4 4 F 5 YMR207C HFA1 - Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis lipid metabolic process ligase activity mitochondrion, cytoplasm 793 BY4741 13_4 4 F 6 YMR210W - - Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification lipid metabolic process transferase activity, transferring acyl groups cellular_component 796 BY4741 13_4 4 F 7 YMR214W SCJ1 - One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins protein folding, proteolysis involved in cellular protein catabolic process, response to chemical stimulus other endoplasmic reticulum, cytoplasm 800 BY4741 13_4 4 F 8 YMR215W GAS3 - Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation biological_process other cell wall, membrane, membrane fraction 801 BY4741 13_4 4 F 9 YMR216C SKY1 - SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 protein targeting, RNA splicing, protein phosphorylation, transmembrane transport, regulation of transport, response to chemical stimulus, cellular ion homeostasis, mRNA processing, nuclear transport kinase activity cytoplasm 802 BY4741 13_4 4 F 10 YMR219W ESC1 - Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p other molecular_function nucleus 805 BY4741 13_4 4 F 11 YMR221C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm 807 BY4741 13_4 4 F 12 YMR222C FSH2 - Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cytoplasm 808 BY4741 13_4 4 G 1 YMR223W UBP8 - Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B protein alkylation, chromatin organization, protein modification by small protein conjugation or removal, histone modification peptidase activity, structural molecule activity nucleus 809 BY4741 13_4 4 G 2 YMR224C MRE11 NGS1|XRS4|RAD58 Subunit of a complex with Rad50p and Xrs2p (MRX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for MRX function; widely conserved meiotic cell cycle, DNA repair, sporulation, response to DNA damage stimulus, DNA recombination kinase activity, DNA binding, structural molecule activity, nuclease activity nucleus, mitochondrion, cytoplasm 810 BY4741 13_4 4 G 3 YMR225C MRPL44 YmL44|YMR44 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 811 BY4741 13_4 4 G 4 YMR226C - TMA29 NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments other oxidoreductase activity nucleus, membrane, ribosome, cytoplasm 812 BY4741 13_4 4 G 5 YMR228W MTF1 - Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; binding of Mtf1p to the core polymerase (Rpo41p)-promoter complex stabilizes the binding and induces DNA bending mitochondrion organization, transcription initiation, DNA-dependent protein binding transcription factor activity mitochondrial envelope, mitochondrion, cytoplasm 814 BY4741 13_4 4 G 6 YMR230W RPS10B S10B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Ap and has similarity to rat ribosomal protein S10 cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 816 BY4741 13_4 4 G 7 YMR231W PEP5 VPT11|VPS11|VPL9|VAM1|END1 Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p vacuole organization, regulation of organelle organization, regulation of transport, organelle fusion, vesicle organization, protein complex biogenesis, Golgi vesicle transport, membrane fusion, endosome transport, exocytosis lipid binding vacuole, membrane, cytoplasm 817 BY4741 13_5 4 G 8 YMR232W FUS2 - Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating cell morphogenesis, organelle fusion, response to chemical stimulus, conjugation, nucleus organization molecular_function nucleus, mitochondrion, site of polarized growth, cytoplasm 818 BY4741 13_5 4 G 9 YMR233W TRI1 - Non-essential sumoylated protein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus biological_process molecular_function nucleus, nucleolus, cytoplasm 819 BY4741 13_5 4 G 10 YMR234W RNH1 - Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) RNA catabolic process, cell wall organization or biogenesis nuclease activity nucleus 820 BY4741 13_5 4 G 11 YMR237W BCH1 - Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes carbohydrate metabolic process, Golgi vesicle transport, cell wall organization or biogenesis molecular_function cytoplasmic membrane-bounded vesicle, ribosome, cytoplasm, Golgi apparatus 823 BY4741 13_5 4 G 12 YMR238W DFG5 - Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p cell budding, pseudohyphal growth, cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds membrane, plasma membrane 824 BY4741 13_5 4 H 1 YMR241W YHM2 - Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome mitochondrion organization, ion transport transmembrane transporter activity, DNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 827 BY4741 13_5 4 H 3 YMR242C RPL20A L20A|L18A|RPL18A2 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similarity to rat L18a ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 828 BY4741 13_5 4 H 4 YMR243C ZRC1 OSR1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; also has a role in resistance to zinc shock resulting from a sudden influx of zinc into the cytoplasm cellular ion homeostasis, ion transport, cellular amino acid metabolic process transmembrane transporter activity membrane, vacuole, cytoplasm 829 BY4741 13_5 4 H 5 YMR244W - - Putative protein of unknown function biological_process molecular_function cellular_component 830 BY4741 13_5 4 H 6 YMR245W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 831 BY4741 13_5 4 H 7 YMR244C-A - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR244C-A is not an essential gene biological_process molecular_function nucleus, cytoplasm 832 BY4741 13_5 4 H 8 YMR246W FAA4 - Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles lipid transport, cofactor metabolic process ligase activity cytoplasm 833 BY4741 13_5 4 H 9 YMR247C RKR1 LTN1 RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus, ribosome, cytoplasm 834 BY4741 13_5 4 H 10 YMR250W GAD1 - Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress response to oxidative stress, response to chemical stimulus, cellular amino acid metabolic process lyase activity cytoplasm 835 BY4741 13_5 4 H 11 YMR251W GTO3 - Omega class glutathione transferase; putative cytosolic localization cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 836 BY4741 13_5 4 H 12 YMR251W-A HOR7 - Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor other molecular_function cell wall, membrane, membrane fraction, endoplasmic reticulum, plasma membrane, cytoplasm 837 BY4741 13_5 5 A 1 YMR252C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 838 BY4741 13_5 5 A 2 YMR253C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene biological_process molecular_function membrane, cytoplasm 839 BY4741 13_5 5 A 3 YMR254C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 840 BY4741 13_5 5 A 4 YMR255W GFD1 - Coiled-coiled protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation nucleobase-containing compound transport, nuclear transport molecular_function nucleus, membrane, endomembrane system, cytoplasm 841 BY4741 13_5 5 A 6 YMR256C COX7 - Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 842 BY4741 13_5 5 A 7 YMR257C PET111 - Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 843 BY4741 13_5 5 A 8 YMR258C ROY1 - GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p protein targeting, membrane invagination, nucleobase-containing small molecule metabolic process, endocytosis enzyme regulator activity nucleus, membrane fraction, cytoplasm 844 BY4741 13_5 5 A 9 YMR259C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene biological_process molecular_function cytoplasm 845 BY4741 13_5 5 A 10 YMR261C TPS3 - Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, phosphatase activity, enzyme regulator activity cytoplasm 847 BY4741 13_5 5 A 11 YMR262W - - Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene biological_process molecular_function cellular_component 848 BY4741 13_5 5 A 12 YNL047C SLM2 LIT1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex endosome transport, cytoskeleton organization, signaling, protein complex biogenesis lipid binding membrane, plasma membrane 7372 BY4741 00_18 5 B 1 YMR264W CUE1 KIS4 Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination proteolysis involved in cellular protein catabolic process enzyme regulator activity membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 850 BY4741 13_5 5 B 2 YMR265C - - Putative protein of unknown function biological_process molecular_function cellular_component 851 BY4741 13_5 5 B 3 YMR266W RSN1 - Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane Golgi vesicle transport molecular_function membrane 852 BY4741 13_5 5 B 4 YMR267W PPA2 IPP2 Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate generation of precursor metabolites and energy, cellular respiration other mitochondrion, cytoplasm 853 BY4741 13_5 5 B 5 YMR269W TMA23 YMR268W-A Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan ribosomal small subunit biogenesis molecular_function nucleus, nucleolus, ribosome, cytoplasm 855 BY4741 13_5 5 B 6 YMR272C SCS7 FAH1 Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm 858 BY4741 13_5 5 B 7 YMR273C ZDS1 OSS1|NRC1|CKM1|CES1 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at the telomere; has a role in Bcy1p localization; implicated in mRNA nuclear export mitotic cell cycle, nucleobase-containing compound transport, nuclear transport, regulation of cell cycle enzyme regulator activity nucleus, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm 859 BY4741 13_5 5 B 8 YMR274C RCE1 - Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone protein maturation peptidase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 860 BY4741 13_5 5 B 9 YMR275C BUL1 SMM2|RDS1|DAG1 Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases protein modification by small protein conjugation or removal, organelle inheritance, mitochondrion organization ligase activity membrane, plasma membrane 861 BY4741 13_5 5 B 10 YMR276W DSK2 - Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome cytoskeleton organization, proteolysis involved in cellular protein catabolic process protein binding, bridging nucleus 862 BY4741 13_5 5 B 11 YMR278W PGM3 - Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential biological_process isomerase activity nucleus, cytoplasm 864 BY4741 13_5 5 B 12 YMR280C CAT8 MSP8|DIL1 Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements carbohydrate metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 866 BY4741 13_5 5 C 1 YMR282C AEP2 ATP13 Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader translational initiation, mitochondrial translation, mitochondrion organization molecular_function mitochondrion, cytoplasm 868 BY4741 13_5 5 C 2 YMR283C RIT1 initiator methionine tRNA 2'-O-ribosyl phosphate transferase 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA tRNA processing, RNA modification transferase activity, transferring glycosyl groups cytoplasm 869 BY4741 13_5 5 C 3 YMR284W YKU70 KU70|NES24|HDF1 Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair DNA repair, telomere organization, chromatin organization, response to DNA damage stimulus, DNA recombination RNA binding, DNA binding nucleus, endomembrane system, chromosome 870 BY4741 13_5 5 C 4 YMR285C NGL2 - Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p and Ngl3p rRNA processing nuclease activity other 871 BY4741 13_5 5 C 5 YMR286W MRPL33 YmL33 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 872 BY4741 13_5 5 C 6 YMR287C DSS1 MSU1 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs RNA catabolic process nuclease activity mitochondrion, cytoplasm 873 BY4741 13_5 5 C 7 YMR289W ABZ2 - Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process lyase activity cytoplasm 875 BY4741 13_5 5 C 8 YMR291W TDA1 - Putative kinase of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process kinase activity nucleus, cytoplasm 878 BY4741 13_5 5 C 9 YMR292W GOT1 - Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Golgi vesicle transport, endosome transport molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 879 BY4741 13_5 5 C 10 YMR293C HER2 RRG6|GEP6|LRC6 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 880 BY4741 13_5 5 C 11 YMR294W JNM1 PAC3|INS1 Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B mitotic cell cycle, cytoskeleton organization structural molecule activity cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 881 BY4741 13_5 5 C 12 YMR294W-A - - Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function cellular_component 882 BY4741 13_5 5 D 1 YMR295C - - Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene biological_process molecular_function cellular bud, site of polarized growth, ribosome, cytoplasm 883 BY4741 13_5 5 D 2 YMR297W PRC1 CPY|LBC1 Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family other peptidase activity vacuole, endoplasmic reticulum, cytoplasm 885 BY4741 13_5 5 D 3 YMR299C DYN3 - Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration other other cytoskeleton, cytoplasm 887 BY4741 13_5 5 D 4 YMR300C ADE4 - Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups cytoplasm 888 BY4741 13_5 5 D 5 YMR302C YME2 PRP12|RNA12 Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 890 BY4741 13_5 5 D 6 YMR303C ADH2 ADR2 Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process oxidoreductase activity cytoplasm 891 BY4741 13_5 5 D 7 YMR304W UBP15 - Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p protein modification by small protein conjugation or removal peptidase activity cytoplasm 892 BY4741 13_5 5 D 8 YMR304C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 biological_process molecular_function cellular_component 893 BY4741 13_5 5 D 9 YMR305C SCW10 - Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p conjugation hydrolase activity, acting on glycosyl bonds cell wall, extracellular region, endoplasmic reticulum, cytoplasm 894 BY4741 13_5 5 D 10 YMR306C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 896 BY4741 13_5 5 D 11 YMR307W GAS1 GGP1|CWH52 Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery cell wall organization or biogenesis other nucleus, membrane fraction, mitochondrion, cell wall, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm, plasma membrane 897 BY4741 13_5 5 D 12 YMR310C - - Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene biological_process methyltransferase activity nucleus 900 BY4741 13_5 5 E 1 YNL339C YRF1-6 YRF1 Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p telomere organization, DNA recombination helicase activity nucleus 1105 BY4741 14_1 5 E 2 YNL338W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV biological_process molecular_function cellular_component 1106 BY4741 14_1 5 E 3 YNL336W COS1 - Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function membrane, vacuole, cytoplasm 1108 BY4741 14_1 5 E 4 YNL335W DDI3 - Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants biological_process molecular_function cellular_component 1109 BY4741 14_1 5 E 5 YNL334C SNO2 - Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin vitamin metabolic process molecular_function cellular_component 1110 BY4741 14_1 5 E 6 YNL333W SNZ2 - Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p vitamin metabolic process other cellular_component 1111 BY4741 14_1 5 E 7 YNL332W THI12 - Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 vitamin metabolic process molecular_function cellular_component 1112 BY4741 14_1 5 E 8 YNL330C RPD3 MOF6|SDS6|SDI2|REC3 Histone deacetylase; regulates transcription, silencing, and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members transcription from RNA polymerase I promoter, response to heat, mitotic cell cycle, meiotic cell cycle, chromatin organization, DNA replication, histone modification, DNA recombination, transcription elongation, DNA-dependent, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle protein binding transcription factor activity, hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, chromosome 1114 BY4741 14_1 5 E 9 YNL329C PEX6 PAS8 AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol protein targeting, peroxisome organization, signaling ATPase activity peroxisome, membrane fraction, cytoplasm 1115 BY4741 14_1 5 E 10 YNL328C MDJ2 - Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, enzyme regulator activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1116 BY4741 14_1 5 E 11 YNL327W EGT2 - Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner cytokinesis hydrolase activity, acting on glycosyl bonds cellular bud, cell wall 1117 BY4741 14_1 5 E 12 YNL326C PFA3 - Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions vacuole organization, protein acylation, membrane fusion, protein lipidation transferase activity, transferring acyl groups vacuole, membrane, cytoplasm 1118 BY4741 14_1 5 F 1 YNL324W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1119 BY4741 14_1 5 F 2 YNL325C FIG4 - Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain lipid metabolic process phosphatase activity membrane, vacuole, cytoplasm 1120 BY4741 14_1 5 F 3 YNL323W LEM3 ROS3|BRE3 Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane lipid transport, signaling ATPase activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 1121 BY4741 14_1 5 F 4 YNL322C KRE1 - Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor cell wall organization or biogenesis structural molecule activity cell wall, membrane 1122 BY4741 14_1 5 F 5 YNL321W VNX1 - Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate ion transport transmembrane transporter activity membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm 1123 BY4741 14_1 5 F 6 YNL320W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1124 BY4741 14_1 5 F 7 YNL319W - - Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 biological_process molecular_function cellular_component 1125 BY4741 14_1 5 F 8 YNL318C HXT14 - Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies carbohydrate transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 1126 BY4741 14_1 5 F 9 YNL314W DAL82 - Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 1130 BY4741 14_1 5 F 10 YNL311C SKP2 - F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments proteolysis involved in cellular protein catabolic process, cellular amino acid metabolic process protein binding, bridging, ligase activity ribosome, cytoplasm 1133 BY4741 14_1 5 F 11 YNL309W STB1 - Protein with a role in regulation of MBF-specific transcription at Start, phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p and binding is required for Stb1p function; expression is cell-cycle regulated mitotic cell cycle, regulation of DNA metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus, chromosome, cytoplasm 1135 BY4741 14_1 5 F 12 YNL053W MSG5 - Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p protein targeting, protein phosphorylation, protein dephosphorylation, regulation of transport, response to chemical stimulus, regulation of protein modification process, conjugation, cell wall organization or biogenesis, nuclear transport, signaling phosphatase activity nucleus, cytoplasm 7373 BY4741 00_18 5 G 1 YNL305C BXI1 YBH3 Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p response to chemical stimulus, signaling molecular_function vacuole, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 1139 BY4741 14_1 5 G 2 YNL304W YPT11 - Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells organelle inheritance, mitochondrion organization GTPase activity cellular bud, site of polarized growth, endoplasmic reticulum, cytoplasm 1140 BY4741 14_1 5 G 3 YNL303W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1141 BY4741 14_1 5 G 4 YNL302C RPS19B rp55B|YS16B|S19B|S16aB|RP55B Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Ap cytoplasmic translation, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 1142 BY4741 14_1 5 G 5 YNL301C RPL18B rp28B|L18B|RP28B Protein component of the large (60S) ribosomal subunit, identical to Rpl18Ap and has similarity to rat L18 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1143 BY4741 14_1 5 G 6 YNL299W TRF5 - Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p RNA catabolic process, snoRNA processing nucleotidyltransferase activity nucleus, nucleolus 1145 BY4741 14_1 5 G 7 YNL298W CLA4 ERC10 Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p vacuole organization, mitotic cell cycle, transmembrane transport, response to chemical stimulus, lipid transport, cell budding, protein phosphorylation, cytokinesis, organelle inheritance, regulation of cell cycle, signaling kinase activity nucleus, vacuole, cellular bud, cytoplasm 1146 BY4741 14_1 5 G 8 YNL086W SNN1 BLS1 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes biological_process molecular_function cytoplasm 7377 BY4741 00_18 5 G 9 YNL297C MON2 YSL2 Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins protein targeting, Golgi vesicle transport, membrane invagination, endosome transport, endocytosis enzyme regulator activity membrane, Golgi apparatus, cytoplasm 1148 BY4741 14_1 5 G 10 YNL295W - - Putative protein of unknown function biological_process molecular_function cellular_component 1149 BY4741 14_1 5 G 11 YNL294C RIM21 PAL2 Component of the RIM101 pathway, has a role in cell wall construction and alkaline pH response; has similarity to A. nidulans PalH invasive growth in response to glucose limitation, sporulation, cell wall organization or biogenesis molecular_function membrane 1150 BY4741 14_1 5 G 12 YNL293W MSB3 GYP3 GTPase-activating protein for Sec4p and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth exocytosis, cytoskeleton organization enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoplasm 1151 BY4741 14_1 5 H 1 YNL089C - - Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins biological_process molecular_function cellular_component 7378 BY4741 00_18 5 H 3 YNL291C MID1 - N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer ion transport transmembrane transporter activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 1153 BY4741 14_1 5 H 4 YNL289W PCL1 HCS26 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth mitotic cell cycle, regulation of protein modification process, protein phosphorylation, cytoskeleton organization, response to starvation, regulation of cell cycle enzyme regulator activity nucleus 1155 BY4741 14_1 5 H 5 YNL288W CAF40 - Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p transcription from RNA polymerase II promoter molecular_function nucleus 1156 BY4741 14_1 5 H 6 YNL286W CUS2 - Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) RNA splicing, mRNA processing RNA binding nucleus 1158 BY4741 14_1 5 H 7 YNL285W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1159 BY4741 14_1 5 H 8 YNL283C WSC2 STA3 Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response response to heat, cytoskeleton organization, cell wall organization or biogenesis, signaling signal transducer activity membrane fraction, cellular bud, site of polarized growth, cytoplasm 1161 BY4741 14_1 5 H 9 YNL281W HCH1 - Heat shock protein regulator that binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus protein folding enzyme regulator activity nucleus, cytoplasm 1163 BY4741 14_1 5 H 10 YNL280C ERG24 - C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm 1164 BY4741 14_1 5 H 11 YNL278W CAF120 - Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation transcription from RNA polymerase II promoter molecular_function nucleus 1166 BY4741 14_1 5 H 12 YNL096C RPS7B rp30|S7B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 7379 BY4741 00_18 6 A 1 YNL276C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W biological_process molecular_function cellular_component 1168 BY4741 14_1 6 A 2 YNL275W BOR1 - Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 protein targeting, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 1169 BY4741 14_1 6 A 3 YNL273W TOF1 - Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase mitotic cell cycle, DNA repair, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 1171 BY4741 14_1 6 A 4 YNL271C BNI1 SHE5|PPF3 Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 mitotic cell cycle, cytoskeleton organization, regulation of organelle organization, cytokinesis, protein complex biogenesis other cell cortex, membrane fraction, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 1173 BY4741 14_1 6 A 5 YNL270C ALP1 APL1 Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression amino acid transport transmembrane transporter activity membrane, plasma membrane 1174 BY4741 14_1 6 A 7 YNL269W BSC4 - Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p biological_process molecular_function cellular_component 1175 BY4741 14_1 6 A 8 YNL268W LYP1 - Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids amino acid transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 1176 BY4741 14_1 6 A 9 YNL266W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C biological_process molecular_function cellular_component 1178 BY4741 14_1 6 A 10 YNL265C IST1 - Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p endosome transport molecular_function cytoplasm 1179 BY4741 14_1 6 A 11 YNL264C PDR17 SFH4|ISS1 Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition lipid transport, lipid metabolic process, response to chemical stimulus other cytoplasm 1180 BY4741 14_1 6 A 12 YNL259C ATX1 - Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake cellular ion homeostasis, ion transport, response to oxidative stress, response to chemical stimulus ion binding cytoplasm 1185 BY4741 14_1 6 B 1 YNL257C SIP3 - Protein that activates transcription through interaction with DNA-bound Snf1p, C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate transcription from RNA polymerase II promoter protein binding transcription factor activity membrane 1187 BY4741 14_1 6 B 2 YNL255C GIS2 - Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 regulation of translation RNA binding, DNA binding, mRNA binding ribosome, cytoplasm 1189 BY4741 14_1 6 B 3 YNL254C RTC4 - Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 1190 BY4741 14_1 6 B 4 YNL253W TEX1 - Protein involved in mRNA export, component of the transcription export (TREX) complex nucleobase-containing compound transport, nuclear transport molecular_function nucleus 1191 BY4741 14_1 6 B 5 YNL249C MPA43 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1195 BY4741 14_1 6 B 6 YNL248C RPA49 A49 RNA polymerase I subunit A49 transcription from RNA polymerase I promoter, transcription elongation, DNA-dependent molecular_function nucleus, nucleolus 1196 BY4741 14_1 6 B 7 YNL246W VPS75 - NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, regulation of organelle organization, histone modification, response to DNA damage stimulus, regulation of protein modification process histone binding, enzyme regulator activity nucleus, chromosome 1198 BY4741 14_1 6 B 8 YOR001W RRP6 - Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay RNA catabolic process, snoRNA processing, rRNA processing nuclease activity nucleus, nucleolus 1777 BY4741 15_1 6 B 9 YOR002W ALG6 - Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease carbohydrate metabolic process, generation of precursor metabolites and energy, cellular respiration, protein glycosylation, lipid metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 1778 BY4741 15_1 6 B 10 YOR003W YSP3 - Putative precursor to the subtilisin-like protease III other peptidase activity cellular_component 1779 BY4741 15_1 6 B 11 YOR005C DNL4 LIG4 DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth response to DNA damage stimulus, DNA repair ligase activity nucleus, chromosome 1781 BY4741 15_1 6 B 12 YOR006C TSR3 - Protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, cytoplasm 1782 BY4741 15_1 6 C 1 YOR007C SGT2 - Glutamine-rich cytoplasmic protein that serves as a scaffold for binding Get4/5p and other proteins required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; has similarity to human cochaperone SGT response to heat, protein targeting molecular_function cytoplasm 1783 BY4741 15_1 6 C 2 YOR008C SLG1 WSC1|HCS77 Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response response to heat, cytoskeleton organization, regulation of transport, peroxisome organization, cell wall organization or biogenesis, response to osmotic stress, membrane invagination, signaling, endocytosis signal transducer activity cell cortex, membrane fraction, cytoskeleton, plasma membrane, cellular bud, membrane, site of polarized growth, cytoplasm 1784 BY4741 15_1 6 C 3 YOR009W TIR4 - Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock biological_process molecular_function cell wall 1785 BY4741 15_1 6 C 4 YOR010C TIR2 SRP2 Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis other molecular_function cell wall 1786 BY4741 15_1 6 C 5 YOR011W AUS1 - Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen lipid transport, transmembrane transport ATPase activity, transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 1787 BY4741 15_1 6 C 6 YOR012W - - Putative protein of unknown function biological_process molecular_function cellular_component 1788 BY4741 15_1 6 C 7 YOR013W IRC11 - Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci DNA recombination molecular_function cellular_component 1789 BY4741 15_1 6 C 8 YOR014W RTS1 SCS1 B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in absence of Cdc55p; homolog of the mammalian B' subunit of PP2A mitotic cell cycle, meiotic cell cycle, protein dephosphorylation, chromosome segregation, regulation of cell cycle phosphatase activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cellular bud, site of polarized growth, cytoplasm 1790 BY4741 15_1 6 C 9 YOR015W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1791 BY4741 15_1 6 C 10 YOR016C ERP4 - Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Golgi vesicle transport molecular_function membrane 1792 BY4741 15_1 6 C 11 YOR017W PET127 - Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane mitochondrion organization molecular_function mitochondrial envelope, mitochondrion, cytoplasm 1793 BY4741 15_1 6 C 12 YOR018W ROD1 ART4 Membrane protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene,zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins membrane invagination, response to chemical stimulus, endocytosis enzyme binding membrane, plasma membrane 1794 BY4741 15_1 6 D 1 YOR019W - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments other molecular_function ribosome, cytoplasm 1795 BY4741 15_1 6 D 2 YOR021C - - Putative protein of unknown function; YOR021C is not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism biological_process molecular_function cellular_component 1797 BY4741 15_1 6 D 3 YOR022C - - Protein with similarity to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other mitochondrion, cytoplasm 1798 BY4741 15_1 6 D 4 YOR023C AHC1 - Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups nucleus 1799 BY4741 15_1 6 D 5 YOR024W - IRC12 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1800 BY4741 15_1 6 D 6 YOR025W HST3 - Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism lipid metabolic process, chromatin organization, histone modification hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, DNA binding nucleus 1801 BY4741 15_1 6 D 7 YOR026W BUB3 - Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle small conjugating protein binding nucleus, chromosome 1802 BY4741 15_1 6 D 8 YOR027W STI1 - Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop protein folding RNA binding, mRNA binding, enzyme regulator activity cytoplasm 1803 BY4741 15_1 6 D 9 YOR028C CIN5 YAP4|HAL6 Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions transcription from RNA polymerase II promoter, response to osmotic stress, response to chemical stimulus DNA binding, transcription factor binding nucleus, cytoplasm 1804 BY4741 15_1 6 D 10 YOR029W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1805 BY4741 15_1 6 D 11 YOR030W DFG16 ZRG12|ECM41 Probable multiple transmembrane protein, involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; required for accumulation of processed Rim101p pseudohyphal growth, protein maturation molecular_function membrane 1806 BY4741 15_1 6 D 12 YOR031W CRS5 - Copper-binding metallothionein, required for wild-type copper resistance response to chemical stimulus ion binding cytoplasm 1807 BY4741 15_1 6 E 1 YOR032C HMS1 - Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant pseudohyphal growth nucleic acid binding transcription factor activity cellular_component 1808 BY4741 15_1 6 E 2 YOR033C EXO1 DHS1 5'-3' exonuclease and flap-endonuclease involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains response to DNA damage stimulus, meiotic cell cycle, DNA repair, telomere organization, DNA recombination nuclease activity nucleus 1809 BY4741 15_1 6 E 3 YOR034C AKR2 - Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p protein acylation, membrane invagination, endocytosis, protein lipidation transferase activity, transferring acyl groups membrane, Golgi apparatus, cytoplasm 1810 BY4741 15_1 6 E 4 YOR035C SHE4 DIM1 Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization cytoskeleton organization cytoskeletal protein binding cytoplasm 1811 BY4741 15_1 6 E 5 YOR036W PEP12 VPT13|VPS6|VPL6 Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin vacuole organization, Golgi vesicle transport, organelle inheritance other cytoplasm, Golgi apparatus 1812 BY4741 15_1 6 E 6 YOR037W CYC2 - Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) mitochondrion organization, protein complex biogenesis oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 1813 BY4741 15_1 6 E 7 YOR038C HIR2 SPT1 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus 1814 BY4741 15_1 6 E 8 YOR039W CKB2 CK2 subunit Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase transcription from RNA polymerase I promoter, response to DNA damage stimulus, protein phosphorylation, transcription from RNA polymerase III promoter enzyme regulator activity nucleus, nucleolus 1815 BY4741 15_1 6 E 9 YOR040W GLO4 - Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate carbohydrate metabolic process other mitochondrion, cytoplasm 1816 BY4741 15_1 6 E 10 YOR041C - SRF5 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component 1817 BY4741 15_1 6 E 11 YOR042W CUE5 - Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process small conjugating protein binding cytoplasm 1818 BY4741 15_1 6 E 12 YOR043W WHI2 - Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression response to heat, membrane invagination, cytoskeleton organization, response to osmotic stress, endocytosis enzyme regulator activity cellular_component 1819 BY4741 15_1 6 F 1 YOR044W IRC23 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endoplasmic reticulum, cytoplasm 1820 BY4741 15_1 6 F 2 YOR045W TOM6 MOM8B|ISP6 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex protein targeting, mitochondrion organization, transmembrane transport, protein complex biogenesis protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1821 BY4741 15_1 6 F 3 YOR047C STD1 SFS3|MSN3 Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] carbohydrate metabolic process, nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter, response to osmotic stress, signaling enzyme regulator activity nucleus, membrane, plasma membrane 1823 BY4741 15_1 6 F 4 YOR049C RSB1 - Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane lipid transport ATPase activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 1825 BY4741 15_1 6 F 5 YOR050C - YOR29-01 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable biological_process molecular_function cellular_component 1826 BY4741 15_1 6 F 6 YOR051C ETT1 NRO1|YOR29-02 Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough other molecular_function nucleus 1827 BY4741 15_1 6 F 7 YOR052C - YOR29-03 Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic biological_process molecular_function nucleus 1828 BY4741 15_1 6 F 8 YOR053W - YOR29-04 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C biological_process molecular_function cellular_component 1829 BY4741 15_1 6 F 9 YOR054C VHS3 YOR29-05 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis cofactor metabolic process, cellular ion homeostasis, nucleobase-containing small molecule metabolic process lyase activity, enzyme regulator activity cytoplasm 1830 BY4741 15_1 6 F 10 YOR055W - YOR29-06 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1831 BY4741 15_1 6 F 11 YOR058C ASE1 YOR29-09 Mitotic spindle midzone localized microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle assembly, cytokinesis, protein complex biogenesis, regulation of cell cycle cytoskeletal protein binding nucleus, cytoskeleton 1834 BY4741 15_1 6 F 12 YOR059C - YOR29-10 Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor RPN4 biological_process molecular_function cytoplasm 1835 BY4741 15_1 6 G 1 YOR061W CKA2 CK2 subunit|YOR29-12 Alpha' catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase transcription from RNA polymerase I promoter, response to DNA damage stimulus, protein phosphorylation, transcription from RNA polymerase III promoter kinase activity nucleus, nucleolus 1837 BY4741 15_1 6 G 2 YOR062C - YOR29-13 Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm 1838 BY4741 15_1 6 G 3 YOR064C YNG1 YOR29-15 Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification histone binding, transferase activity, transferring acyl groups nucleus 1840 BY4741 15_1 6 G 4 YOR065W CYT1 cytochrome c1|YOR29-16|CTC1 Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex generation of precursor metabolites and energy, cellular respiration other membrane, mitochondrial envelope, mitochondrion, cytoplasm 1841 BY4741 15_1 6 G 5 YOR066W MSA1 YOR29-17 Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation mitotic cell cycle, transcription from RNA polymerase II promoter molecular_function nucleus, cytoplasm 1842 BY4741 15_1 6 G 6 YOR067C ALG8 YOR29-18 Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p carbohydrate metabolic process, protein glycosylation, lipid metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1843 BY4741 15_1 6 G 7 YOR068C VAM10 YOR29-19 Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering vacuole organization, membrane fusion molecular_function membrane, vacuole, cytoplasm 1844 BY4741 15_1 6 G 8 YOR069W VPS5 YOR29-20|VPT5|PEP10|GRD2 Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p endosome transport protein transporter activity, lipid binding membrane, endomembrane system, cytoplasm 1845 BY4741 15_1 6 G 9 YOR070C GYP1 YOR29-21 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion other enzyme regulator activity mitochondrion, cytoplasm, Golgi apparatus 1846 BY4741 15_1 6 G 10 YOR071C NRT1 THI71|YOR29-22 High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 1847 BY4741 15_1 6 G 11 YOR072W - YOR29-23 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive biological_process molecular_function cellular_component 1848 BY4741 15_1 6 G 12 YOR073W SGO1 YOR29-24 Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle molecular_function nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 1849 BY4741 15_1 6 H 1 YOR076C SKI7 YOR29-27 Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype RNA catabolic process protein binding, bridging cytoplasm 1852 BY4741 15_1 6 H 3 YOR078W BUD21 YOR29-29|UTP16 Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 1854 BY4741 15_1 6 H 4 YOR079C ATX2 YOR29-30 Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation cellular ion homeostasis transmembrane transporter activity membrane, endomembrane system, cytoplasm, Golgi apparatus 1855 BY4741 15_1 6 H 5 YOR080W DIA2 YOR29-31 Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth invasive growth in response to glucose limitation, DNA replication, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process ligase activity, DNA binding nucleus, chromosome 1856 BY4741 15_1 6 H 6 YOR081C TGL5 STC2 Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate lipid metabolic process transferase activity, transferring acyl groups cytoplasm 1857 BY4741 15_1 6 H 7 YOR082C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W biological_process molecular_function membrane 1858 BY4741 15_1 6 H 8 YOR083W WHI5 - Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 mitotic cell cycle, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 1859 BY4741 15_1 6 H 9 YOR084W LPX1 - Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction peroxisome organization other peroxisome, cytoplasm 1860 BY4741 15_1 6 H 10 YOR085W OST3 - Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins carbohydrate metabolic process, protein complex biogenesis, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1861 BY4741 15_1 6 H 11 YOR086C TCB1 - Lipid-binding ER protein, enriched at ER-plasma membrane contact sites; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding membrane, cell cortex, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm 1862 BY4741 15_1 6 H 12 YOR087W YVC1 TRPY1|YOR088W Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 1863 BY4741 15_1 7 A 1 YOR088W - - - - - - 1864 BY4741 15_1 7 A 2 YOR089C VPS21 YPT51|YPT21|VPT12|VPS12 Rab family GTPase required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog vacuole organization, protein targeting, membrane invagination, organelle inheritance, endocytosis GTPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1865 BY4741 15_1 7 A 3 YOR090C PTC5 PPP1 Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p protein dephosphorylation phosphatase activity mitochondrion, cytoplasm 1866 BY4741 15_1 7 A 4 YOR091W TMA46 RBF46 Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p cytoplasmic translation molecular_function ribosome, cytoplasm 1867 BY4741 15_1 7 A 5 YOR092W ECM3 YOR3165W Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation cell wall organization or biogenesis ATPase activity membrane, endoplasmic reticulum, cytoplasm 1868 BY4741 15_1 7 A 6 YOR093C - - Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function membrane 1869 BY4741 15_1 7 A 8 YOR094W ARF3 ARL2 Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity cytoskeleton organization, cytokinesis, lipid metabolic process, membrane invagination, endocytosis RNA binding, GTPase activity, mRNA binding cellular bud, site of polarized growth 1870 BY4741 15_1 7 A 9 YOR289W - - Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 1585 BY4741 15_4 7 A 10 YOR290C SNF2 TYE3|SWI2|HAF1|GAM1 Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p response to DNA damage stimulus, DNA repair, chromatin organization, transcription from RNA polymerase II promoter histone binding, ATPase activity nucleus 1586 BY4741 15_4 7 A 11 YOR291W YPK9 - Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome biological_process molecular_function membrane, vacuole, cytoplasm 1587 BY4741 15_4 7 A 12 YOR292C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 1588 BY4741 15_4 7 B 1 YOR293W RPS10A S10A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Bp and has similarity to rat ribosomal protein S10 cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1589 BY4741 15_4 7 B 2 YOR295W UAF30 - Subunit of UAF (upstream activation factor), which is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate transcription from RNA polymerase I promoter, chromatin organization nucleic acid binding transcription factor activity, DNA binding nucleus, nucleolus 1591 BY4741 15_4 7 B 3 YOR296W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene biological_process molecular_function cytoplasm 1592 BY4741 15_4 7 B 4 YOR297C TIM18 - Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex protein targeting, mitochondrion organization, response to oxidative stress, response to chemical stimulus, response to osmotic stress protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1593 BY4741 15_4 7 B 5 YOR298W MUM3 - Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases lipid metabolic process, sporulation, cell wall organization or biogenesis transferase activity, transferring acyl groups cellular_component 1594 BY4741 15_4 7 B 6 YOR299W BUD7 - Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane carbohydrate metabolic process, Golgi vesicle transport, cytokinesis, cell wall organization or biogenesis molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 1595 BY4741 15_4 7 B 7 YOR301W RAX1 - Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily cytokinesis molecular_function cellular bud, membrane, site of polarized growth 1597 BY4741 15_4 7 B 8 YOR302W - - CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation other cytoplasm 1598 BY4741 15_4 7 B 9 YOR303W CPA1 - Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader cellular amino acid metabolic process ligase activity cytoplasm 1599 BY4741 15_4 7 B 10 YOR304C-A - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck biological_process molecular_function cellular bud, site of polarized growth, cytoplasm 1600 BY4741 15_4 7 B 11 YOR304W ISW2 - ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth chromatin organization, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter, response to chemical stimulus DNA binding, ATPase activity nucleus 1601 BY4741 15_4 7 B 12 YOR305W RRG7 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 1602 BY4741 15_4 7 C 1 YOR307C SLY41 - Protein involved in ER-to-Golgi transport Golgi vesicle transport molecular_function membrane, endoplasmic reticulum, cytoplasm 1604 BY4741 15_4 7 C 2 YOR308C SNU66 - Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 RNA splicing, rRNA processing, mRNA processing molecular_function nucleus 1605 BY4741 15_4 7 C 3 YOR311C DGK1 HSD1 Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain lipid metabolic process kinase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1608 BY4741 15_4 7 C 4 YOR312C RPL20B L20B|L18B|RPL18A1 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similarity to rat L18a ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1609 BY4741 15_4 7 C 5 YOR313C SPS4 - Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage sporulation molecular_function cellular_component 1610 BY4741 15_4 7 C 6 YOR314W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1611 BY4741 15_4 7 C 7 YOR315W SFG1 - Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate mitotic cell cycle, pseudohyphal growth molecular_function nucleus, cytoplasm 1612 BY4741 15_4 7 C 8 YOR316C COT1 - Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium cellular ion homeostasis, ion transport transmembrane transporter activity membrane, vacuole, mitochondrion, cytoplasm 1613 BY4741 15_4 7 C 9 YOR318C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo biological_process molecular_function cellular_component 1615 BY4741 15_4 7 C 10 YOR320C GNT1 - N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus 1617 BY4741 15_4 7 C 11 YOR321W PMT3 - Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; target for new antifungals protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 1618 BY4741 15_4 7 C 12 YOR322C LDB19 ART1 Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; localization changes in response to nutrient levels; null mutant has reduced affinity for alcian blue dye membrane invagination, endocytosis enzyme binding cytoplasmic membrane-bounded vesicle, cytoplasm 1619 BY4741 15_4 7 D 1 YOR323C PRO2 - Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 1620 BY4741 15_4 7 D 2 YOR324C FRT1 HPH1 Tail-anchored ER membrane protein that is a substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation other molecular_function endoplasmic reticulum, cytoplasm 1621 BY4741 15_4 7 D 3 YOR327C SNC2 - Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, membrane invagination, exocytosis, endocytosis other endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 1624 BY4741 15_4 7 D 4 YOR328W PDR10 - ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p transmembrane transport, response to chemical stimulus ATPase activity, transmembrane transporter activity membrane, plasma membrane 1625 BY4741 15_4 7 D 5 YOR330C MIP1 mitochondrial DNA polymerase catalytic subunit Catalytic subunit of the mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases DNA replication, mitochondrion organization DNA binding, nucleotidyltransferase activity, nuclease activity mitochondrion, cytoplasm 1627 BY4741 15_4 7 D 6 YOR332W VMA4 - Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 1629 BY4741 15_4 7 D 7 YOR334W MRS2 - Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 1631 BY4741 15_4 7 D 8 YOR337W TEA1 - Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein other DNA binding nucleus 1634 BY4741 15_4 7 D 9 YOR338W - - Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 1635 BY4741 15_4 7 D 10 YOR339C UBC11 - Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 protein modification by small protein conjugation or removal ligase activity cytoplasm 1636 BY4741 15_4 7 D 11 YOR342C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 1639 BY4741 15_4 7 D 12 YOR343C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1640 BY4741 15_4 7 E 1 YOR344C TYE7 SGC1 Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression carbohydrate metabolic process, generation of precursor metabolites and energy nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 1641 BY4741 15_4 7 E 2 YOR346W REV1 - Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; response to DNA damage stimulus, DNA repair nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm 1643 BY4741 15_4 7 E 3 YOR347C PYK2 - Pyruvate kinase that appears to be modulated by phosphorylation; PYK2 transcription is repressed by glucose, and Pyk2p may be active under low glycolytic flux other kinase activity mitochondrion, cytoplasm 1644 BY4741 15_4 7 E 4 YOR348C PUT4 - Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells amino acid transport transmembrane transporter activity membrane, plasma membrane 1645 BY4741 15_4 7 E 5 YOR349W CIN1 - Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl protein folding cytoskeletal protein binding cellular_component 1646 BY4741 15_4 7 E 6 YOR350C MNE1 - Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA RNA splicing molecular_function mitochondrion, cytoplasm 1647 BY4741 15_4 7 E 7 YOR351C MEK1 MRE4 Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids meiotic cell cycle, protein phosphorylation, regulation of cell cycle kinase activity nucleus 1648 BY4741 15_4 7 E 8 YOR352W - - Putative protein of unknown function; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm 1649 BY4741 15_4 7 E 9 YOR354C MSC6 - Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies meiotic cell cycle, DNA recombination molecular_function mitochondrion, cytoplasm 1651 BY4741 15_4 7 E 10 YOR355W GDS1 - Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrion, cytoplasm 1652 BY4741 15_4 7 E 11 YOR356W CIR2 - Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response biological_process molecular_function mitochondrion, cytoplasm 1653 BY4741 15_4 7 E 12 YOR357C SNX3 GRD19 Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Golgi vesicle transport, endosome transport lipid binding cytoplasm 1654 BY4741 15_4 7 F 1 YOR358W HAP5 - Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, response to chemical stimulus, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus 1655 BY4741 15_4 7 F 2 YOR359W VTS1 - Post-transcriptional gene regulator, flap-structured DNA-binding and RNA-binding protein; shows genetic interactions with Vti1p, a v-SNARE involved in cis-Golgi membrane traffic; stimulates Dna2p endonuclease activity; contains a SAM domain RNA catabolic process, mRNA processing RNA binding, DNA binding nucleus, cytoplasm 1656 BY4741 15_4 7 F 3 YOR360C PDE2 SRA5 High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon signaling other nucleus, cytoplasm 1657 BY4741 15_4 7 F 4 YOR363C PIP2 OAF2 Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes lipid metabolic process, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, membrane 1660 BY4741 15_4 7 F 5 YOR365C - - Putative protein of unknown function; YOR365C is not an essential protein biological_process molecular_function membrane 1662 BY4741 15_4 7 F 6 YOR367W SCP1 - Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin cytoskeleton organization protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm 1664 BY4741 15_4 7 F 7 YOR368W RAD17 - Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination, regulation of cell cycle DNA binding nucleus, chromosome 1665 BY4741 15_4 7 F 8 YOR371C GPB1 KRH2 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; homolog of Gpb2p invasive growth in response to glucose limitation, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, protein modification by small protein conjugation or removal, pseudohyphal growth, signaling enzyme regulator activity, signal transducer activity membrane, cytoplasm, plasma membrane 1668 BY4741 15_4 7 F 9 YOR374W ALD4 ALDH2|ALD7 Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, cytoplasm 1671 BY4741 15_4 7 F 10 YOR375C GDH1 DHE4|GDHA|GDH-A|URE1 NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 1672 BY4741 15_4 7 F 11 YOR376W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. biological_process molecular_function membrane 1673 BY4741 15_4 7 F 12 YOR377W ATF1 - Alcohol acetyltransferase with potential roles in lipid and sterol metabolism; responsible for the major part of volatile acetate ester production during fermentation lipid metabolic process, generation of precursor metabolites and energy transferase activity, transferring acyl groups endomembrane system, cytoplasm 1674 BY4741 15_4 7 G 1 YOR378W - - Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene biological_process molecular_function membrane 1675 BY4741 15_4 7 G 2 YOR380W RDR1 - Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 1677 BY4741 15_4 7 G 3 YOR381W FRE3 - Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels cellular ion homeostasis, ion transport oxidoreductase activity membrane, plasma membrane 1678 BY4741 15_4 7 G 4 YOR382W FIT2 - Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall cellular ion homeostasis, ion transport molecular_function cell wall 1679 BY4741 15_4 7 G 5 YOR383C FIT3 - Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall cellular ion homeostasis, ion transport molecular_function cell wall 1680 BY4741 15_4 7 G 6 YOR384W FRE5 - Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process oxidoreductase activity membrane, mitochondrion, cytoplasm 1681 BY4741 15_5 7 G 7 YOR385W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene biological_process molecular_function cytoplasm 1682 BY4741 15_5 7 G 8 YOR386W PHR1 - DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p DNA repair, response to DNA damage stimulus RNA binding, lyase activity, mRNA binding nucleus, mitochondrion, cytoplasm 1683 BY4741 15_5 7 G 9 YOL001W PHO80 phoR|VAC5|TUP7|AGS3 Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling protein phosphorylation, cellular ion homeostasis, regulation of protein modification process, response to starvation, transcription from RNA polymerase II promoter, regulation of cell cycle, signaling enzyme regulator activity nucleus 1692 BY4741 15_5 7 G 10 YOL002C IZH2 PHO36 Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc lipid metabolic process, cellular ion homeostasis, response to chemical stimulus ion binding membrane, plasma membrane 1693 BY4741 15_5 7 G 11 YOL003C PFA4 - Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases protein acylation, protein lipidation transferase activity, transferring acyl groups membrane, endoplasmic reticulum, cytoplasm 1694 BY4741 15_5 7 G 12 YOL004W SIN3 UME4|SDS16|SDI1|RPD1|GAM2|CPE1 Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity transcription from RNA polymerase I promoter, response to heat, mitotic cell cycle, meiotic cell cycle, DNA repair, chromatin organization, DNA replication, histone modification, response to DNA damage stimulus, regulation of DNA metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus, chromosome 1695 BY4741 15_5 7 H 1 YOL006C TOP1 MAK17|MAK1 Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination mitotic cell cycle, organelle fission, chromatin organization, DNA replication, chromosome segregation, DNA recombination, transcription elongation, DNA-dependent, regulation of DNA metabolic process, transcription from RNA polymerase II promoter isomerase activity, DNA binding nucleus, chromosome, nucleolus 1697 BY4741 15_5 7 H 3 YOL007C CSI2 - Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene biological_process molecular_function cellular bud, site of polarized growth, vacuole, cytoplasm 1698 BY4741 15_5 7 H 4 YOL008W COQ10 - Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes generation of precursor metabolites and energy, cellular respiration other membrane, mitochondrial envelope, mitochondrion, cytoplasm 1699 BY4741 15_5 7 H 5 YOL009C MDM12 - Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins protein targeting, mitochondrion organization, lipid transport, organelle inheritance molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 1700 BY4741 15_5 7 H 6 YOL011W PLB3 - Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro lipid metabolic process other membrane, extracellular region, plasma membrane 1702 BY4741 15_5 7 H 7 YOL012C HTZ1 H2AZ|H2A.F/Z|HTA3 Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin RNA catabolic process, transcription from RNA polymerase II promoter other nucleus, chromosome 1703 BY4741 15_5 7 H 8 YOL013C HRD1 DER3 Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger cell wall organization or biogenesis, proteolysis involved in cellular protein catabolic process ligase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1704 BY4741 15_5 7 H 9 YOL014W - - Putative protein of unknown function biological_process molecular_function cellular_component 1705 BY4741 15_5 7 H 10 YOL015W IRC10 - Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component 1706 BY4741 15_5 7 H 11 YOL017W ESC8 - Protein involved in telomeric and mating-type locus silencing, interacts with Sir2p and also interacts with the Gal11p, which is a component of the RNA pol II mediator complex other molecular_function nucleus 1708 BY4741 15_5 7 H 12 YOL018C TLG2 - Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) protein targeting, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion, lipid metabolic process, membrane invagination, endocytosis other membrane, Golgi apparatus, cytoplasm 1709 BY4741 15_5 8 A 1 YOL019W - TOS7 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole biological_process molecular_function membrane, vacuole, plasma membrane, cytoplasm 1710 BY4741 15_5 8 A 2 YOL020W TAT2 TAP2|SCM2|SAB2|LTG3 High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance amino acid transport transmembrane transporter activity membrane, plasma membrane 1711 BY4741 15_5 8 A 3 YOL023W IFM1 - Mitochondrial translation initiation factor 2 translational initiation, mitochondrial translation, mitochondrion organization RNA binding, GTPase activity, translation factor activity, nucleic acid binding mitochondrion, cytoplasm 1714 BY4741 15_5 8 A 4 YOL024W - - Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site biological_process molecular_function cellular_component 1715 BY4741 15_5 8 A 5 YOL025W LAG2 ECM36 Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 regulation of protein modification process, protein modification by small protein conjugation or removal molecular_function mitochondrion, cytoplasm 1716 BY4741 15_5 8 A 6 YOL027C MDM38 MKH1 Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, ion transport, protein complex biogenesis, cellular ion homeostasis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 1718 BY4741 15_5 8 A 7 YOL028C YAP7 - Putative basic leucine zipper (bZIP) transcription factor transcription from RNA polymerase II promoter DNA binding nucleus 1719 BY4741 15_5 8 A 9 YOL029C - - Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function cellular_component 1720 BY4741 15_5 8 A 10 YOL030W GAS5 - 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall biological_process other cell wall, membrane, membrane fraction 1721 BY4741 15_5 8 A 11 YOL031C SIL1 SLS1 Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein protein targeting, transmembrane transport enzyme regulator activity endoplasmic reticulum, cytoplasm 1722 BY4741 15_5 8 A 12 YOL032W OPI10 - Protein with a possible role in phospholipid biosynthesis, based on inositol-excreting phenotype of the null mutant and its suppression by exogenous choline carbohydrate metabolic process molecular_function nucleus, cytoplasm 1723 BY4741 15_5 8 B 1 YOL033W MSE1 mitochondrial glutamyl-tRNA synthetase Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 1724 BY4741 15_5 8 B 2 YOL035C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1726 BY4741 15_5 8 B 3 YOL036W - - Protein of unknown function; potential Cdc28p substrate biological_process molecular_function cellular_component 1727 BY4741 15_5 8 B 4 YOL037C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W biological_process molecular_function cellular_component 1728 BY4741 15_5 8 B 5 YOL039W RPP2A RPLA2|RPL44 Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1730 BY4741 15_5 8 B 6 YOL041C NOP12 - Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p rRNA processing, ribosomal large subunit biogenesis RNA binding nucleus, nucleolus 1732 BY4741 15_5 8 B 7 YOL042W NGL1 - Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process nuclease activity mitochondrion, cytoplasm 1733 BY4741 15_5 8 B 8 YOL043C NTG2 SCR2 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus; sumoylated response to DNA damage stimulus, DNA repair lyase activity, hydrolase activity, acting on glycosyl bonds nucleus 1734 BY4741 15_5 8 B 9 YOL044W PEX15 PAS21 Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly protein targeting, peroxisome organization, signaling other peroxisome, membrane, cytoplasm 1735 BY4741 15_5 8 B 10 YOL045W PSK2 - One of two (see also PSK1) PAS domain containing S/T protein kinases; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation) carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity mitochondrion, cytoplasm 1736 BY4741 15_5 8 B 11 YOL046C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W biological_process molecular_function cellular_component 1737 BY4741 15_5 8 B 12 YOL047C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function membrane, cytoplasm 1738 BY4741 15_5 8 C 1 YOL048C RRT8 - Putative protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles biological_process molecular_function membrane, cytoplasm 1739 BY4741 15_5 8 C 2 YOL049W GSH2 - Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock cellular amino acid metabolic process ligase activity other 1740 BY4741 15_5 8 C 3 YOL050C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) biological_process molecular_function cellular_component 1741 BY4741 15_5 8 C 4 YOL051W GAL11 MED15|ABE1|SPT13|SDS4|RAR3 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 1742 BY4741 15_5 8 C 5 YOL052C SPE2 - S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process lyase activity nucleus, cytoplasm 1743 BY4741 15_5 8 C 6 YOL053C-A - - - - - - 1744 BY4741 15_5 8 C 7 YOL053W AIM39 - Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 1745 BY4741 15_5 8 C 8 YOL054W PSH1 - E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus, chromosome 1746 BY4741 15_5 8 C 9 YOL055C THI20 - Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p vitamin metabolic process kinase activity, hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm 1747 BY4741 15_5 8 C 10 YOL056W GPM3 - Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process molecular_function, isomerase activity cytoplasm 1748 BY4741 15_5 8 C 11 YOL057W - - Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers other peptidase activity nucleus, cytoplasm 1749 BY4741 15_5 8 C 12 YOL058W ARG1 ARG10 Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate cellular amino acid metabolic process ligase activity cytoplasm 1750 BY4741 15_5 8 D 1 YOL059W GPD2 GPD3 NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, cytoplasm 1751 BY4741 15_5 8 D 2 YOL060C MAM3 - Protein required for normal mitochondrial morphology, has similarity to hemolysins cellular ion homeostasis, mitochondrion organization molecular_function membrane, vacuole, cytoplasm 1752 BY4741 15_5 8 D 3 YOL061W PRS5 - 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes carbohydrate metabolic process, cell wall organization or biogenesis other cytoplasm 1753 BY4741 15_5 8 D 4 YOL062C APM4 AMP1 Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, plasma membrane, cytoplasm 1754 BY4741 15_5 8 D 5 YOL063C CRT10 HUS1 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat transcription from RNA polymerase II promoter molecular_function cellular_component 1755 BY4741 15_5 8 D 6 YOL064C MET22 3'(2')5'-bisphosphate nucleotidase|HAL2 Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway response to osmotic stress, cellular amino acid metabolic process phosphatase activity cellular_component 1756 BY4741 15_5 8 D 7 YOL065C INP54 - Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins lipid metabolic process phosphatase activity endoplasmic reticulum, cytoplasm 1757 BY4741 15_5 8 D 8 YOL067C RTG1 - Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus transcription from RNA polymerase II promoter, signaling, response to chemical stimulus nucleic acid binding transcription factor activity nucleus, cytoplasm 1759 BY4741 15_5 8 D 9 YOL068C HST1 - NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance chromatin organization, DNA recombination, regulation of DNA metabolic process, histone modification hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, chromosome 1760 BY4741 15_5 8 D 10 YOL070C NBA1 - Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate biological_process molecular_function cellular bud, site of polarized growth, ribosome, cytoplasm 1762 BY4741 15_5 8 D 11 YOL071W EMI5 SDH5 Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) cofactor metabolic process, sporulation, generation of precursor metabolites and energy, cellular respiration, transcription from RNA polymerase II promoter oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1763 BY4741 15_5 8 D 12 YOL072W THP1 BUD29 Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding DNA repair, cytokinesis, nucleobase-containing compound transport, response to DNA damage stimulus, RNA catabolic process, transcription elongation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport RNA binding, DNA binding nucleus, endomembrane system, membrane 1764 BY4741 15_5 8 E 1 YOL075C - - Putative ABC transporter other ATPase activity, transmembrane transporter activity membrane 1766 BY4741 15_5 8 E 2 YOL076W MDM20 NAA25|DEC1 Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly peptidyl-amino acid modification, protein acylation, organelle inheritance, mitochondrion organization, cytoskeleton organization transferase activity, transferring acyl groups cytoplasm 1767 BY4741 15_5 8 E 3 YOL079W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 1770 BY4741 15_5 8 E 4 YOL080C REX4 - Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis nuclease activity nucleus, nucleolus 1771 BY4741 15_5 8 E 5 YOL081W IRA2 GLC4|CCS1 GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin nucleobase-containing small molecule metabolic process, signaling enzyme regulator activity membrane, mitochondrion, cytoplasm 1772 BY4741 15_5 8 E 6 YOL082W ATG19 CVT19 Receptor protein specific for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Lap4p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles protein targeting, vesicle organization, proteolysis involved in cellular protein catabolic process, protein maturation protein binding, bridging membrane fraction, cytoplasm 1773 BY4741 15_5 8 E 7 YOL083W ATG34 ATG19-B Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog protein complex biogenesis protein binding, bridging cytoplasm 1774 BY4741 15_5 8 E 8 YOL084W PHM7 - Protein of unknown function, expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole biological_process molecular_function membrane, vacuole, cytoplasm 1775 BY4741 15_5 8 E 9 YOL085C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A biological_process molecular_function cellular_component 1776 BY4741 15_5 8 E 10 YPL274W SAM3 - High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p transmembrane transport, amino acid transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 1018 BY4741 16_1 8 E 11 YPL273W SAM4 - S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process methyltransferase activity nucleus, cytoplasm 1019 BY4741 16_1 8 E 12 YPL272C - - Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene biological_process molecular_function cellular_component 1020 BY4741 16_1 8 F 1 YPL271W ATP15 ATPEPSILON Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1021 BY4741 16_1 8 F 2 YPL270W MDL2 - Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis transmembrane transport ATPase activity, transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 1022 BY4741 16_1 8 F 3 YPL269W KAR9 - Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase other molecular_function cell cortex, site of polarized growth, cytoskeleton, microtubule organizing center, cytoplasm 1023 BY4741 16_1 8 F 4 YPL267W ACM1 - Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein mitotic cell cycle, regulation of protein modification process, protein modification by small protein conjugation or removal enzyme regulator activity nucleus, cytoplasm 1025 BY4741 16_1 8 F 5 YPL265W DIP5 - Dicarboxylic amino acid permease, mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly amino acid transport transmembrane transporter activity membrane, plasma membrane 1027 BY4741 16_1 8 F 6 YPL264C - - Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene biological_process molecular_function membrane 1028 BY4741 16_1 8 F 7 YPL263C KEL3 - Cytoplasmic protein of unknown function biological_process molecular_function cytoplasm 1029 BY4741 16_1 8 F 8 YPL262W FUM1 - Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration lyase activity mitochondrion, cytoplasm 1030 BY4741 16_1 8 F 9 YPL260W - - Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene biological_process molecular_function nucleus, cytoplasm 1031 BY4741 16_1 8 F 10 YPL261C - - Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W biological_process molecular_function cellular_component 1032 BY4741 16_1 8 F 11 YPL259C APM1 YAP54 Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 1033 BY4741 16_1 8 F 12 YPL258C THI21 - Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p vitamin metabolic process kinase activity cellular_component 1034 BY4741 16_1 8 G 1 YPL257W - - Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene biological_process molecular_function membrane 1035 BY4741 16_1 8 G 2 YPL256C CLN2 - G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) protein phosphorylation, response to chemical stimulus, regulation of protein modification process, conjugation, signaling, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 1036 BY4741 16_1 8 G 3 YPL254W HFI1 GAN1|SRM12|SUP110|ADA1 Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification protein binding transcription factor activity nucleus 1038 BY4741 16_1 8 G 4 YPL253C VIK1 - Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p mitotic cell cycle, organelle fission, chromosome segregation other cytoskeleton, microtubule organizing center, cytoplasm 1039 BY4741 16_1 8 G 5 YPL250C ICY2 - Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate other molecular_function cellular_component 1042 BY4741 16_1 8 G 6 YPL249C GYP5 - GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth Golgi vesicle transport enzyme regulator activity membrane fraction, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 1043 BY4741 16_1 8 G 7 YPL248C GAL4 GAL81 DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p carbohydrate metabolic process, response to chemical stimulus, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus 1044 BY4741 16_1 8 G 8 YPL247C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest biological_process molecular_function nucleus, cytoplasm 1045 BY4741 16_1 8 G 9 YPL246C RBD2 - Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p biological_process molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 1046 BY4741 16_1 8 G 10 YPL245W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus, cytoplasm 1047 BY4741 16_1 8 G 11 YPL244C HUT1 - Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 transmembrane transport, carbohydrate transport transmembrane transporter activity membrane 1048 BY4741 16_1 8 G 12 YPL241C CIN2 - GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 protein folding enzyme regulator activity cellular_component 1051 BY4741 16_1 8 H 1 YPL240C HSP82 HSP90 Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p telomere organization, protein targeting, DNA replication, mitochondrion organization, regulation of organelle organization, protein complex biogenesis, protein folding, regulation of DNA metabolic process, response to osmotic stress unfolded protein binding, ATPase activity cytoplasm 1052 BY4741 16_1 8 H 3 YPL239W YAR1 - Cytoplasmic ankyrin-repeat containing protein of unknown function, proposed to link the processes of 40S ribosomal subunit biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock ribosomal small subunit biogenesis, response to oxidative stress, response to osmotic stress, response to chemical stimulus molecular_function cytoplasm 1053 BY4741 16_1 8 H 4 YPL236C ENV7 - Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; has sequence similarity with the human serine/threonine protein kinase STK16 protein maturation kinase activity membrane, vacuole, cytoplasm 1056 BY4741 16_1 8 H 5 YPL234C VMA11 TFP3|CLS9 Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 1058 BY4741 16_1 8 H 6 YPL232W SSO1 - Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p sporulation, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion lipid binding membrane fraction, vacuole, membrane, cytoplasm, plasma membrane 1060 BY4741 16_1 8 H 7 YPL230W USV1 NSF1 Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis transcription from RNA polymerase II promoter, response to osmotic stress, response to chemical stimulus DNA binding nucleus 1062 BY4741 16_1 8 H 8 YPL229W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL229W is not an essential gene biological_process molecular_function cytoplasm 1063 BY4741 16_1 8 H 9 YPL227C ALG5 - UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1065 BY4741 16_1 8 H 10 YPL226W NEW1 - ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction nucleobase-containing compound transport, nuclear transport, ribosomal small subunit biogenesis ATPase activity mitochondrion, cytoplasm 1066 BY4741 16_1 8 H 11 YPL225W - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function ribosome, cytoplasm 1067 BY4741 16_1 8 H 12 YPL223C GRE1 - Hydrophilin of unknown function; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway biological_process molecular_function cytoplasm 1069 BY4741 16_1 9 A 1 YPL222W FMP40 - Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1070 BY4741 16_1 9 A 2 YPL221W FLC1 BOP1|HUF1 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance nucleobase-containing compound transport, protein folding, cell wall organization or biogenesis transmembrane transporter activity vacuole, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm 1071 BY4741 16_1 9 A 3 YPL220W RPL1A L1A|SSM1 N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1072 BY4741 16_1 9 A 4 YPL219W PCL8 - Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other 1073 BY4741 16_1 9 A 5 YPL216W - - Putative protein of unknown function; YPL216W is not an essential gene biological_process molecular_function cellular_component 1076 BY4741 16_1 9 A 6 YPL215W CBP3 - Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1077 BY4741 16_1 9 A 7 YPL214C THI6 - Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern vitamin metabolic process kinase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 1078 BY4741 16_1 9 A 8 YPL213W LEA1 - Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein RNA splicing, mRNA processing molecular_function nucleus, cytoplasm 1079 BY4741 16_1 9 A 10 YPL212C PUS1 - tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA tRNA processing, RNA modification isomerase activity nucleus 1080 BY4741 16_1 9 A 11 YPL208W RKM1 - SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit protein L23a (RPL23A and RPL23B) peptidyl-amino acid modification, protein alkylation methyltransferase activity nucleus, cytoplasm 1084 BY4741 16_1 9 A 12 YPL207W TYW1 - Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron tRNA processing, nucleobase-containing small molecule metabolic process, RNA modification molecular_function endoplasmic reticulum, cytoplasm 1085 BY4741 16_1 9 B 1 YPL206C PGC1 - Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs lipid metabolic process other mitochondrion, cytoplasm 1086 BY4741 16_1 9 B 2 YPL205C - - Hypothetical protein; deletion of locus affects telomere length sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 1087 BY4741 16_1 9 B 3 YPL203W TPK2 PKA3|YKR1|PKA2 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase protein phosphorylation, invasive growth in response to glucose limitation, signaling kinase activity nucleus 1089 BY4741 16_1 9 B 4 YPL202C AFT2 - Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; similar to Aft1p response to oxidative stress, response to chemical stimulus, cellular ion homeostasis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, mitochondrion, cytoplasm 1090 BY4741 16_1 9 B 5 YPL201C YIG1 - Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol carbohydrate metabolic process, generation of precursor metabolites and energy molecular_function nucleus, cytoplasm 1091 BY4741 16_1 9 B 6 YPL200W CSM4 - Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements meiotic cell cycle, chromosome segregation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, endomembrane system, chromosome, membrane, endoplasmic reticulum, cytoplasm 1092 BY4741 16_1 9 B 7 YPL199C - - Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cytoplasm 1093 BY4741 16_1 9 B 8 YPL198W RPL7B YL8|L7B|L6B|rp11 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) cytoplasmic translation structural molecule activity, structural constituent of ribosome nucleus, nucleolus, ribosome, cytoplasm 1094 BY4741 16_1 9 B 9 YPL197C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B biological_process molecular_function cellular_component 1095 BY4741 16_1 9 B 10 YPL196W OXR1 - Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes response to oxidative stress, response to chemical stimulus molecular_function mitochondrion, cytoplasm 1096 BY4741 16_1 9 B 11 YPL195W APL5 YKS4 Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Golgi vesicle transport other membrane, cytoplasm 1097 BY4741 16_1 9 B 12 YPL194W DDC1 - DNA damage checkpoint protein, part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate mitotic cell cycle, meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus, chromosome 1098 BY4741 16_1 9 C 1 YPL193W RSA1 - Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus 1099 BY4741 16_1 9 C 2 YPL192C PRM3 - Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body organelle fusion, membrane fusion, conjugation, nucleus organization molecular_function nucleus, cytoskeleton, microtubule organizing center, endomembrane system, membrane, cytoplasm 1100 BY4741 16_1 9 C 3 YPL191C - - Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 1101 BY4741 16_1 9 C 4 YPL189W GUP2 - Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog carbohydrate transport transferase activity, transferring acyl groups membrane 1103 BY4741 16_1 9 C 5 YPL188W POS5 - Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to oxidative stress, response to chemical stimulus kinase activity mitochondrion, cytoplasm 1104 BY4741 16_1 9 C 6 YPL187W MF(ALPHA)1 alpha factor Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor conjugation, signaling, response to chemical stimulus other extracellular region 2065 BY4741 16_2 9 C 7 YPL185W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C biological_process molecular_function membrane 2066 BY4741 16_2 9 C 8 YPL186C UIP4 - Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope biological_process molecular_function nucleus, membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 2067 BY4741 16_2 9 C 9 YPL184C MRN1 PTR69 RNA-binding protein proposed to be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES) biological_process molecular_function cytoplasm 2068 BY4741 16_2 9 C 10 YPL181W CTI6 RXT1 Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain DNA replication, regulation of DNA metabolic process histone binding, transcription factor binding nucleus, chromosome 2070 BY4741 16_2 9 C 11 YPL182C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W biological_process molecular_function cellular_component 2071 BY4741 16_2 9 C 12 YPL180W TCO89 - Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin carbohydrate metabolic process, cell wall organization or biogenesis, signaling, response to osmotic stress molecular_function vacuole, membrane, cytoplasm, plasma membrane 2072 BY4741 16_2 9 D 1 YPL179W PPQ1 SAL6 Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors regulation of translation, protein dephosphorylation phosphatase activity cytoplasm 2073 BY4741 16_2 9 D 2 YPL178W CBC2 CBP20|SAE1|MUD13 Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif RNA splicing, mRNA processing RNA binding nucleus 2074 BY4741 16_2 9 D 3 YPL177C CUP9 - Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription regulation of transport, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus 2075 BY4741 16_2 9 D 4 YPL176C TRE1 - Plasma membrane protein that binds to Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; function is redundant with that of Tre2p; has similarity to transferrin receptors protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process molecular_function vacuole, cytoplasm 2076 BY4741 16_2 9 D 5 YPL174C NIP100 PAC13 Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) mitotic cell cycle, cytoskeleton organization cytoskeletal protein binding cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 2078 BY4741 16_2 9 D 6 YPL173W MRPL40 YmL40 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 2079 BY4741 16_2 9 D 7 YPL172C COX10 - Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders cofactor metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups membrane, mitochondrion, cytoplasm 2080 BY4741 16_2 9 D 8 YPL171C OYE3 Old Yellow Enzyme|ZRG6 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death other oxidoreductase activity cellular_component 2081 BY4741 16_2 9 D 9 YPL170W DAP1 - Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis lipid metabolic process ion binding membrane, cytoplasm 2082 BY4741 16_2 9 D 10 YPL168W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated biological_process molecular_function mitochondrion, cytoplasm 2084 BY4741 16_2 9 D 11 YPL167C REV3 PSO1 Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair response to DNA damage stimulus, DNA repair nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm 2085 BY4741 16_2 9 D 12 YPL166W ATG29 - Autophagy-specific protein that is required for recruitment of other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved membrane invagination, response to starvation molecular_function cytoplasm 2086 BY4741 16_2 9 E 1 YPL165C SET6 - SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability biological_process molecular_function cellular_component 2087 BY4741 16_2 9 E 2 YPL164C MLH3 - Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination molecular_function nucleus 2088 BY4741 16_2 9 E 3 YPL163C SVS1 - Cell wall and vacuolar protein, required for wild-type resistance to vanadate response to chemical stimulus molecular_function cell wall, vacuole, cytoplasm 2089 BY4741 16_2 9 E 4 YPL162C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology biological_process molecular_function membrane, vacuole, cytoplasm 2090 BY4741 16_2 9 E 5 YPL161C BEM4 ROM7 Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length cytoskeleton organization, signaling molecular_function nucleus, cytoplasm 2091 BY4741 16_2 9 E 6 YPL159C PET20 - Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome generation of precursor metabolites and energy, cellular respiration molecular_function mitochondrion, cytoplasm 2093 BY4741 16_2 9 E 7 YPL157W TGS1 - Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs meiotic cell cycle, telomere organization, DNA replication, regulation of organelle organization, regulation of DNA metabolic process, RNA modification, nucleus organization methyltransferase activity nucleus, nucleolus 2095 BY4741 16_2 9 E 8 YPL156C PRM4 - Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function membrane 2096 BY4741 16_2 9 E 9 YPL155C KIP2 - Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization other cytoskeleton, mitochondrion, cytoplasm 2097 BY4741 16_2 9 E 10 YPL154C PEP4 yscA|PRA1|PHO9 Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates membrane invagination, response to starvation, proteolysis involved in cellular protein catabolic process peptidase activity vacuole, mitochondrion, cytoplasm 2098 BY4741 16_2 9 E 11 YPL152W RRD2 YPA2 Activator of the phosphotyrosyl phosphatase activity of PP2A,peptidyl-prolyl cis/trans-isomerase; regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex mitotic cell cycle, cytoskeleton organization, response to osmotic stress isomerase activity, enzyme regulator activity cytoplasm 2100 BY4741 16_2 9 E 12 YPL150W - - Putative protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study biological_process kinase activity cellular_component 2102 BY4741 16_2 9 F 1 YPL149W ATG5 APG5 Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation protein targeting, membrane invagination, response to starvation, protein lipidation ligase activity vacuole, cytoplasm 2103 BY4741 16_2 9 F 2 YPL147W PXA1 SSH2|PAT2|PAL1|LPI1 Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins lipid transport ATPase activity, transmembrane transporter activity peroxisome, membrane, cytoplasm 2105 BY4741 16_2 9 F 3 YPL145C KES1 BSR3|OSH4|LPI3 Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex Golgi vesicle transport, lipid transport, membrane invagination, exocytosis, endocytosis lipid binding endomembrane system, membrane, Golgi apparatus, cytoplasm 2107 BY4741 16_2 9 F 4 YPL144W POC4 DMP1|PBA4 Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions protein complex biogenesis molecular_function cytoplasm 2108 BY4741 16_2 9 F 5 YPL141C FRK1 - Putative protein kinase; similar to Kin4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3 biological_process kinase activity cytoplasm 2111 BY4741 16_2 9 F 6 YPL140C MKK2 SSP33|LPI6 Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p protein phosphorylation, peroxisome organization, signaling kinase activity other 2112 BY4741 16_2 9 F 7 YPL139C UME1 WTM3 Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p transcription from RNA polymerase I promoter, response to heat, meiotic cell cycle, transcription from RNA polymerase II promoter, regulation of cell cycle protein binding transcription factor activity nucleus, chromosome 2113 BY4741 16_2 9 F 8 YPL138C SPP1 SAF41|CPS40 Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein protein alkylation, chromatin organization, histone modification histone binding, methyltransferase activity nucleus 2114 BY4741 16_2 9 F 9 YPL136W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C biological_process molecular_function cellular_component 2115 BY4741 16_2 9 F 10 YPL135W ISU1 NUA1 Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable cofactor metabolic process, cellular ion homeostasis, tRNA processing, RNA modification ion binding mitochondrion, cytoplasm 2117 BY4741 16_2 9 F 11 YPL133C RDS2 - Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole carbohydrate metabolic process, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 2119 BY4741 16_2 9 F 12 YPL130W SPO19 - Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation meiotic cell cycle, sporulation molecular_function cell wall, membrane 2122 BY4741 16_2 9 G 1 YPL129W TAF14 TafII30|ANC1|TFG3|TAF30|SWP29 Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes, involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity nucleus, chromosome 2123 BY4741 16_2 9 G 2 YPL127C HHO1 - Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation DNA recombination, regulation of DNA metabolic process DNA binding nucleus, chromosome 2125 BY4741 16_2 9 G 3 YPL125W KAP120 LPH2 Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p protein targeting, nuclear transport protein transporter activity, structural molecule activity nucleus, endomembrane system, membrane, cytoplasm 2127 BY4741 16_2 9 G 4 YPL123C RNY1 - Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity RNA catabolic process, cell morphogenesis nuclease activity extracellular region, vacuole, cytoplasm 2129 BY4741 16_2 9 G 5 YPL121C MEI5 LPH6 Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination molecular_function nucleus, chromosome 2131 BY4741 16_2 9 G 6 YPL120W VPS30 ATG6|VPT30|APG6 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; ortholog of the higher eukaryotic gene Beclin 1 protein targeting, proteolysis involved in cellular protein catabolic process, peroxisome organization, lipid metabolic process, membrane invagination, endosome transport, response to starvation kinase activity membrane fraction, cytoplasm 2132 BY4741 16_2 9 G 7 YPL119C DBP1 LPH8 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs translational initiation helicase activity cytoplasm 2133 BY4741 16_2 9 G 8 YPL118W MRP51 - Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 2134 BY4741 16_2 9 G 9 YPL116W HOS3 - Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats mitotic cell cycle, chromatin organization, histone modification, transcription from RNA polymerase II promoter hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cellular bud, site of polarized growth, cytoplasm 2136 BY4741 16_2 9 G 10 YPL115C BEM3 - Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly signaling lipid binding, enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoplasm 2137 BY4741 16_2 9 G 11 YPL114W - - Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index biological_process molecular_function cellular_component 2138 BY4741 16_2 9 G 12 YPL113C - - Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene other oxidoreductase activity cellular_component 2139 BY4741 16_2 9 H 1 YPL112C PEX25 - Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p protein targeting, peroxisome organization molecular_function peroxisome, membrane, cytoplasm 2140 BY4741 16_2 9 H 3 YPL111W CAR1 cargA|LPH15 Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance cellular amino acid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ion binding, enzyme regulator activity nucleus, site of polarized growth, cytoplasm 2141 BY4741 16_2 9 H 4 YPL110C GDE1 - Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes lipid metabolic process other ribosome, cytoplasm 2142 BY4741 16_2 9 H 5 YPL109C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 2143 BY4741 16_2 9 H 6 YPL108W - - Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cytoplasm 2144 BY4741 16_2 9 H 7 YPL107W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 2145 BY4741 16_2 9 H 8 YPL106C SSE1 MSI3|LPG3 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm protein folding enzyme regulator activity cytoplasm 2146 BY4741 16_2 9 H 9 YPL105C SYH1 MYR1 Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan nucleus organization molecular_function membrane, ribosome, mitochondrion, cytoplasm 2147 BY4741 16_2 9 H 10 YPL104W MSD1 mitochondrial aspartyl-tRNA synthetase|LPG5 Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 2148 BY4741 16_2 9 H 11 YPL103C FMP30 - Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D lipid metabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm 2149 BY4741 16_2 9 H 12 YPL101W ELP4 KTI9|TOT7|HAP1 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity tRNA processing, transcription from RNA polymerase II promoter, RNA modification molecular_function cytoplasm 2150 BY4741 16_2 10 A 1 YPL102C - - Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 biological_process molecular_function cellular_component 2151 BY4741 16_2 10 A 2 YPL100W ATG21 HSV1|MAI1 Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein protein targeting, vesicle organization, membrane invagination, response to starvation, protein lipidation lipid binding vacuole, membrane, cytoplasm 2152 BY4741 16_2 10 A 3 YPL099C AIM43 FMP14 Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 2153 BY4741 16_2 10 A 4 YPL098C MGR2 - Protein required for growth of cells lacking the mitochondrial genome biological_process molecular_function cellular_component 2154 BY4741 16_2 10 A 5 YPL097W MSY1 - Mitochondrial tyrosyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 2155 BY4741 16_2 10 A 6 YPL096W PNG1 - Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p proteolysis involved in cellular protein catabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, mitochondrion, cytoplasm 2156 BY4741 16_2 10 A 7 YPL095C EEB1 - Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification lipid metabolic process transferase activity, transferring acyl groups cellular_component 2157 BY4741 16_2 10 A 8 YPL092W SSU1 LPG16 Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein ion transport transmembrane transporter activity membrane, plasma membrane 2160 BY4741 16_2 10 A 9 YBR174C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective biological_process molecular_function cellular_component 3314 BY4741 02_4 10 A 11 YBR175W SWD3 SAF35|CPS30 Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 3315 BY4741 02_4 10 A 12 YBR176W ECM31 - Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process other mitochondrion, cytoplasm 3316 BY4741 02_4 10 B 1 YBR177C EHT1 - Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane lipid metabolic process transferase activity, transferring acyl groups membrane, mitochondrial envelope, mitochondrion, cytoplasm 3317 BY4741 02_4 10 B 2 YBR178W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C biological_process molecular_function cellular_component 3318 BY4741 02_4 10 B 3 YBR179C FZO1 - Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system mitochondrion organization, organelle fusion, membrane fusion GTPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 3319 BY4741 02_4 10 B 4 YBR180W DTR1 - Putative dityrosine transporter, required for spore wall synthesis; expressed during sporulation; member of the major facilitator superfamily (DHA1 family) of multidrug resistance transporters sporulation, transmembrane transport, cell wall organization or biogenesis transmembrane transporter activity membrane 3320 BY4741 02_4 10 B 5 YBR181C RPS6B RPS102|RPS101|LPG18 Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 3321 BY4741 02_4 10 B 6 YBR182C SMP1 - Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 3322 BY4741 02_4 10 B 7 YBR183W YPC1 alkaline ceramidase Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance lipid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, transferase activity, transferring acyl groups, hydrolase activity, acting on glycosyl bonds membrane, endoplasmic reticulum, cytoplasm 3323 BY4741 02_4 10 B 8 YBR184W - - Putative protein of unknown function; YBR184W is not an essential gene biological_process molecular_function cellular_component 3324 BY4741 02_4 10 B 9 YBR185C MBA1 - Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 3325 BY4741 02_4 10 B 10 YBR186W PCH2 - Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA meiotic cell cycle, regulation of cell cycle molecular_function nucleus, nucleolus 3326 BY4741 02_4 10 B 11 YBR187W GDT1 - Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis biological_process molecular_function membrane, vacuole, cytoplasm 3327 BY4741 02_4 10 B 12 YBR188C NTC20 - Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs RNA splicing, mRNA processing other nucleus 3328 BY4741 02_4 10 C 1 YBR194W AIM4 SOY1 Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress biological_process molecular_function cytoplasm 3334 BY4741 02_4 10 C 2 YBR195C MSI1 CAC3 Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase chromatin organization histone binding nucleus, cytoplasm 3335 BY4741 02_4 10 C 3 YBR197C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene biological_process molecular_function nucleus, cytoplasm 3337 BY4741 02_4 10 C 4 YBR199W KTR4 - Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus 3339 BY4741 02_4 10 C 5 YBR200W BEM1 SRO1 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p cell morphogenesis, conjugation, response to chemical stimulus structural molecule activity, lipid binding cellular bud, site of polarized growth 3340 BY4741 02_4 10 C 6 YBR201W DER1 - Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 3341 BY4741 02_4 10 C 7 YBR203W COS111 - Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signaling molecular_function mitochondrion, cytoplasm 3343 BY4741 02_4 10 C 8 YBR204C LDH1 - Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) other other cytoplasm 3344 BY4741 02_4 10 C 9 YBR205W KTR3 - Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles protein glycosylation, carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups vacuole, cytoplasm 3345 BY4741 02_4 10 C 10 YBR206W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 biological_process molecular_function cellular_component 3346 BY4741 02_4 10 C 11 YBR207W FTH1 - Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis transmembrane transport, ion transport, membrane invagination, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm 3347 BY4741 02_4 10 C 12 YBR208C DUR1,2 DUR80 Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ligase activity cytoplasm 3348 BY4741 02_4 10 D 1 YBR209W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene biological_process molecular_function cellular_component 3349 BY4741 02_4 "Hypothetical protein -- growth on -met, no growth on -lys, no growth on drop-in media" 10 D 2 YBR210W ERV15 - Protein involved in export of proteins from the endoplasmic reticulum, has similarity to Erv14p Golgi vesicle transport, cell budding, cytokinesis molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm 3350 BY4741 02_4 10 D 3 YBR212W NGR1 RBP1 RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase mitochondrion organization RNA binding, mRNA binding cytoplasm 3352 BY4741 02_4 10 D 4 YBR213W MET8 - Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis cofactor metabolic process lyase activity, oxidoreductase activity cellular_component 3353 BY4741 02_4 10 D 5 YBR214W SDS24 - One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; may play an indirect role in fluid-phase endocytosis membrane invagination, cytokinesis, endocytosis molecular_function cytoplasm 3354 BY4741 02_4 10 D 6 YBR215W HPC2 - Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes mitotic cell cycle, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter DNA binding nucleus 3355 BY4741 02_4 10 D 7 YBR216C YBP1 - Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress response to oxidative stress, response to chemical stimulus molecular_function cytoplasm 3356 BY4741 02_4 10 D 8 YBR217W ATG12 APG12 Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation vacuole organization, protein targeting, organelle assembly, membrane invagination, response to starvation, protein lipidation ligase activity membrane fraction, cytoplasm 3357 BY4741 02_4 10 D 9 YBR218C PYC2 - Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process ligase activity cytoplasm 3358 BY4741 02_4 10 D 10 YBR219C - - Putative protein of unknown function; YBR219C is not an essential gene biological_process molecular_function membrane 3359 BY4741 02_4 10 D 11 YBR220C - - Putative protein of unknown function; YBR220C is not an essential gene biological_process molecular_function membrane 3360 BY4741 02_4 10 D 12 YBR221C PDB1 - E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria cofactor metabolic process oxidoreductase activity mitochondrion, cytoplasm 3361 BY4741 02_4 10 E 1 YBR222C PCS60 FAT2 Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase biological_process RNA binding, mRNA binding peroxisome, membrane, cytoplasm 3362 BY4741 02_4 10 E 2 YBR223C TDP1 - Tyrosyl-DNA Phosphodiesterase I, hydrolyzes 3' and 5'-phosphotyrosyl bonds, involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI response to DNA damage stimulus, DNA repair other nucleus 3363 BY4741 02_4 10 E 3 YBR224W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 biological_process molecular_function membrane 3364 BY4741 02_4 10 E 4 YBR225W - - Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components biological_process molecular_function cellular_component 3365 BY4741 02_4 10 E 5 YBR226C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W biological_process molecular_function cellular_component 3366 BY4741 02_4 10 E 6 YBR227C MCX1 - Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein folding molecular_function mitochondrion, cytoplasm 3367 BY4741 02_4 10 E 7 YBR228W SLX1 - Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA replication nuclease activity nucleus, chromosome 3368 BY4741 02_4 10 E 8 YBR229C ROT2 GLS2 Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds mitochondrion, endoplasmic reticulum, cytoplasm 3369 BY4741 02_4 10 E 9 YBR230C OM14 - Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3370 BY4741 02_4 10 E 10 YBR231C SWC5 AOR1 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin organization molecular_function nucleus, chromosome 3371 BY4741 02_4 10 E 11 YBR233W PBP2 HEK1 RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length telomere organization RNA binding, mRNA binding cytoplasm 3373 BY4741 02_4 10 E 12 YBR235W - - Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene transmembrane transport transmembrane transporter activity membrane 3375 BY4741 02_4 10 F 1 YBR238C - - Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span generation of precursor metabolites and energy, cellular respiration molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3378 BY4741 02_4 10 F 2 YBR239C ERT1 - Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition biological_process DNA binding nucleus, cytoplasm 3379 BY4741 02_4 10 F 3 YBR240C THI2 phoF|PHO6 Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type vitamin metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 3380 BY4741 02_4 10 F 4 YBR241C - - Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm 3381 BY4741 02_4 10 F 5 YBR242W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene biological_process molecular_function nucleus, cytoplasm 3382 BY4741 02_4 10 F 6 YBR244W GPX2 AMI1 Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 3384 BY4741 02_4 10 F 7 YBR245C ISW1 SGN2 Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation transcription from RNA polymerase I promoter, chromatin organization, protein complex biogenesis, transcription initiation, DNA-dependent, transcription termination, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter DNA binding, ATPase activity nucleus, chromosome, nucleolus 3385 BY4741 02_4 10 F 8 YBR246W RRT2 ERE1 Diphthamide synthetase; required for last step of diphthamide biosynthesis, deletion leads to accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription peptidyl-amino acid modification, endosome transport ligase activity cytoplasm 3386 BY4741 02_4 10 F 9 YBR248C HIS7 - Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, transferring glycosyl groups other 3388 BY4741 02_4 10 F 10 YBR249C ARO4 - 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups nucleus, cytoplasm 3389 BY4741 02_4 10 F 11 YBR250W SPO23 - Protein of unknown function; associates with meiosis-specific protein Spo1p meiotic cell cycle molecular_function cellular_component 3390 BY4741 02_4 10 F 12 YBR251W MRPS5 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3391 BY4741 02_4 10 G 1 YBR255W MTC4 - Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm 3395 BY4741 02_4 10 G 2 YBR258C SHG1 CPS15 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 3398 BY4741 02_4 10 G 3 YBR259W - - Putative protein of unknown function; YBR259W is not an essential gene biological_process molecular_function cellular_component 3399 BY4741 02_4 10 G 4 YBR260C RGD1 - GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization response to chemical stimulus, signaling, response to osmotic stress lipid binding, enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 3400 BY4741 02_4 10 G 5 YBR261C TAE1 NTM1 AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm peptidyl-amino acid modification, cytoplasmic translation, protein alkylation methyltransferase activity cytoplasm 3401 BY4741 02_4 10 G 6 YBR262C AIM5 MCS12|FMP51 Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3402 BY4741 02_4 10 G 7 YBR263W SHM1 TMP3|SHMT1 Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine cellular amino acid metabolic process other mitochondrion, cytoplasm 3403 BY4741 02_4 10 G 8 YBR264C YPT10 - Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles other GTPase activity cytoplasm 3404 BY4741 02_4 10 G 9 YBR266C SLM6 - Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase membrane invagination, endocytosis molecular_function cellular_component 3406 BY4741 02_4 10 G 10 YBR267W REI1 - Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network mitotic cell cycle, cell budding, ribosomal large subunit biogenesis, nuclear transport DNA binding ribosome, cytoplasm 3407 BY4741 02_4 Incorrect -- PCR mating type A/Alpha 10 G 11 YBR268W MRPL37 YmL37 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3408 BY4741 02_4 10 G 12 YDR049W VMS1 - Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 3985 BY4741 04_4 10 H 1 YDR050C TPI1 - Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease carbohydrate metabolic process, generation of precursor metabolites and energy isomerase activity membrane fraction, mitochondrion, cytoplasm 3986 BY4741 04_4 10 H 3 YDR051C DET1 - Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel lipid transport phosphatase activity nucleus, cytoplasm 3987 BY4741 04_4 10 H 4 YDR055W PST1 HPF2 Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 cell wall organization or biogenesis molecular_function cell wall, membrane, plasma membrane 3991 BY4741 04_4 10 H 5 YDR056C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein biological_process molecular_function membrane fraction, endoplasmic reticulum, cytoplasm 3992 BY4741 04_4 10 H 6 YDR057W YOS9 - ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family proteolysis involved in cellular protein catabolic process other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 3993 BY4741 04_4 10 H 7 YDR059C UBC5 - Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible protein modification by small protein conjugation or removal ligase activity other 3994 BY4741 04_4 10 H 8 YDR061W - - Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance biological_process molecular_function mitochondrion, cytoplasm 3996 BY4741 04_4 10 H 9 YDR063W AIM7 GMF1|GMF|Glia maturation factor Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function nucleus, cell cortex, cytoskeleton, cytoplasm 3998 BY4741 04_4 10 H 10 YDR065W RRG1 - Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cellular ion homeostasis, mitochondrion organization molecular_function mitochondrion, cytoplasm 4000 BY4741 04_4 10 H 11 YDR066C RTR2 - Protein of unknown function with high similarity to Rtr1p; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene biological_process molecular_function cytoplasm 4001 BY4741 04_4 10 H 12 YDR067C OCA6 - Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT biological_process molecular_function cytoplasm 4002 BY4741 04_4 11 A 1 YDR068W DOS2 - Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 4003 BY4741 04_4 11 A 2 YDR069C DOA4 UBP4|SSV7|NPI2|MUT4|DOS1 Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole DNA replication, proteolysis involved in cellular protein catabolic process, membrane invagination, protein modification by small protein conjugation or removal, regulation of DNA metabolic process, endocytosis peptidase activity membrane fraction, mitochondrion, cytoplasm 4004 BY4741 04_4 11 A 3 YDR070C FMP16 - Putative protein of unknown function; proposed to be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 4005 BY4741 04_4 11 A 4 YDR072C IPT1 KTI6|SYR4 Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid;, can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin lipid metabolic process other membrane, membrane fraction 4007 BY4741 04_4 11 A 5 YDR073W SNF11 - Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p chromatin organization molecular_function nucleus 4008 BY4741 04_4 11 A 6 YDR075W PPH3 - Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes meiotic cell cycle, DNA repair, protein dephosphorylation, response to DNA damage stimulus, DNA recombination, signaling, regulation of cell cycle phosphatase activity nucleus, chromosome, cytoplasm 4010 BY4741 04_4 11 A 7 YDR076W RAD55 - Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination ATPase activity nucleus 4011 BY4741 04_4 11 A 8 YDR077W SED1 - Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites mitochondrion organization, cell wall organization or biogenesis structural molecule activity cell wall, ribosome, mitochondrion, cytoplasm 4012 BY4741 04_4 11 A 9 YDR078C SHU2 - Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function response to DNA damage stimulus, DNA repair, DNA recombination molecular_function other 4013 BY4741 04_4 11 A 10 YDR079W PET100 - Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme mitochondrion organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 4014 BY4741 04_4 11 A 12 YDR080W VPS41 VPL20|VAM2|SVL2|FET2|CVT8 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport vacuole organization, regulation of organelle organization, regulation of transport, organelle fusion, vesicle organization, protein maturation, protein complex biogenesis, membrane fusion, membrane invagination lipid binding, enzyme regulator activity vacuole, membrane, cytoplasm 4015 BY4741 04_4 11 B 1 YDR083W RRP8 - Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation rRNA processing methyltransferase activity nucleus, nucleolus 4018 BY4741 04_4 11 B 2 YDR084C TVP23 - Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern other molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus 4019 BY4741 04_4 11 B 3 YDR085C AFR1 - Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p cell morphogenesis, conjugation, signaling, response to chemical stimulus signal transducer activity other 4020 BY4741 04_4 11 B 4 YDR089W - - Protein of unknown function; deletion confers resistance to Nickel other molecular_function membrane 4024 BY4741 04_4 11 B 5 YDR090C - - Putative protein of unknown function biological_process molecular_function membrane 4025 BY4741 04_4 11 B 6 YDR092W UBC13 - Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus response to DNA damage stimulus, DNA repair, protein modification by small protein conjugation or removal, protein complex biogenesis ligase activity nucleus, cytoplasm 4027 BY4741 04_4 11 B 7 YDR093W DNF2 - Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase lipid transport, membrane invagination, endocytosis ATPase activity membrane, membrane fraction, plasma membrane 4028 BY4741 04_4 11 B 8 YDR094W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 biological_process molecular_function cellular_component 4029 BY4741 04_4 11 B 9 YDR095C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4030 BY4741 04_4 11 B 10 YDR096W GIS1 - JmjC domain-containing histone demethylase and transcription factor; involved in expression of genes during nutrient limitation; negatively regulates DPP1 and PHR1; activity is modulated by limited proteasome-mediated proteolysis; has a JmjC and a JmjN domain in the N-terminal region that interact, promoting Gis1p stability and proper transcriptional activity; contains transactivating domains TAD1 and TAD2 downstream of the Jmj domains and a C-terminal DNA binding domain chromatin organization, histone modification, lipid metabolic process, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, oxidoreductase activity, DNA binding nucleus, mitochondrion, cytoplasm 4031 BY4741 04_4 11 B 11 YDR097C MSH6 PMS3 Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p response to DNA damage stimulus, meiotic cell cycle, DNA repair DNA binding, ATPase activity nucleus 4032 BY4741 04_4 11 B 12 YDR098C GRX3 - Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage cellular ion homeostasis, cytoskeleton organization, response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 4033 BY4741 04_4 11 C 1 YDR099W BMH2 SCD3 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling mitotic cell cycle, carbohydrate metabolic process, DNA replication, sporulation, generation of precursor metabolites and energy, response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, pseudohyphal growth, cell wall organization or biogenesis, signaling, regulation of cell cycle DNA binding nucleus, membrane fraction 4034 BY4741 04_4 11 C 2 YDR100W TVP15 - Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 4035 BY4741 04_4 11 C 3 YDR101C ARX1 - Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex ribosomal large subunit biogenesis molecular_function nucleus, ribosome, cytoplasm 4036 BY4741 04_4 11 C 4 YDR102C - - Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index biological_process molecular_function cellular_component 4037 BY4741 04_4 11 C 5 YDR103W STE5 NUL3|HMD3 Pheromone-response scaffold protein that controls the mating decision; binds Ste11p, Ste7p, and Fus3p kinases, forming a MAPK cascade complex that interacts with the plasma membrane and Ste4p-Ste18p; allosteric activator of Fus3p protein phosphorylation, invasive growth in response to glucose limitation, transposition, response to chemical stimulus, regulation of protein modification process, conjugation, signaling lipid binding, structural molecule activity nucleus, membrane, site of polarized growth, cytoplasm, plasma membrane 4038 BY4741 04_4 11 C 6 YDR104C SPO71 - Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis sporulation, cell wall organization or biogenesis molecular_function cell wall 4039 BY4741 04_4 11 C 7 YDR105C TMS1 - Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance biological_process molecular_function membrane, vacuole, cytoplasm 4040 BY4741 04_4 11 C 8 YDR107C TMN2 - Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments cellular ion homeostasis, invasive growth in response to glucose limitation, pseudohyphal growth molecular_function membrane, vacuole, cytoplasm 4041 BY4741 04_4 11 C 9 YDR108W TRS85 GSG1|MUM1 Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role meiotic cell cycle, protein targeting, vesicle organization, peroxisome organization, Golgi vesicle transport, membrane invagination, response to starvation molecular_function Golgi apparatus, cytoplasm 4042 BY4741 04_4 11 C 10 YDR109C - - Putative kinase biological_process molecular_function cellular_component 4043 BY4741 04_4 11 C 11 YDR110W FOB1 HRM1 Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases mitotic cell cycle, organelle fission, chromatin organization, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process DNA binding nucleus, nucleolus 4044 BY4741 04_4 11 C 12 YDR111C ALT2 - Putative alanine transaminase (glutamic pyruvic transaminase) biological_process other nucleus, cytoplasm 4045 BY4741 04_4 11 D 1 YDR112W IRC2 - Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component 4046 BY4741 04_4 11 D 2 YDR114C - - Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium biological_process molecular_function cellular_component 4048 BY4741 04_4 11 D 3 YDR115W - - Putative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4049 BY4741 04_4 11 D 4 YDR116C MRPL1 - Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4050 BY4741 04_4 11 D 5 YDR117C TMA64 RBF64 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity biological_process RNA binding ribosome, cytoplasm 4051 BY4741 04_4 11 D 6 YDR119W VBA4 - Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function membrane, vacuole, cytoplasm 4053 BY4741 04_4 11 D 7 YDR120C TRM1 - tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments tRNA processing, RNA modification methyltransferase activity nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 4054 BY4741 04_4 11 D 8 YDR121W DPB4 - Shared subunit of DNA polymerase (II) epsilon and of ISW2/yCHRAC chromatin accessibility complex; involved in both chromosomal DNA replication and in inheritance of telomeric silencing DNA replication DNA binding, nucleotidyltransferase activity nucleus, chromosome 4055 BY4741 04_4 11 D 9 YDR122W KIN1 - Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p protein phosphorylation, exocytosis kinase activity membrane, plasma membrane 4056 BY4741 04_4 11 D 10 YDR123C INO2 SCS1|DIE1 Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 4057 BY4741 04_4 11 D 11 YDR124W - - Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor biological_process molecular_function cellular_component 4058 BY4741 04_4 11 D 12 YDR125C ECM18 - Protein of unknown function, similar to Rlp24p cell wall organization or biogenesis molecular_function mitochondrion, cytoplasm 4059 BY4741 04_4 11 E 1 YDR126W SWF1 PSL10 Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion vacuole organization, protein acylation, sporulation, cytoskeleton organization, regulation of transport, membrane fusion, exocytosis, cell wall organization or biogenesis, protein lipidation transferase activity, transferring acyl groups cell cortex, cytoskeleton, endomembrane system, membrane, cytoplasm 4060 BY4741 04_4 11 E 2 YDR127W ARO1 - Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids cellular amino acid metabolic process lyase activity, kinase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups, oxidoreductase activity cytoplasm 4061 BY4741 04_4 11 E 3 YDR128W MTC5 SEA3 Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1 biological_process molecular_function membrane, vacuole, cytoplasm 4062 BY4741 04_4 11 E 4 YDR129C SAC6 ABP67 Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton cell budding, cytoskeleton organization, cytokinesis, membrane invagination, response to osmotic stress, endocytosis protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm 4063 BY4741 04_4 11 E 5 YDR130C FIN1 - Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in mother and daughter cells; localization cell-cycle dependent; involved in Glc7p localization and regulation mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 4064 BY4741 04_4 11 E 6 YDR131C - - F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex proteolysis involved in cellular protein catabolic process protein binding, bridging, ligase activity other 4065 BY4741 04_4 11 E 7 YDR132C - - Putative protein of unknown function biological_process molecular_function nucleus, cytoplasm 4066 BY4741 04_4 11 E 8 YDR133C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C biological_process molecular_function cellular_component 4067 BY4741 04_4 11 E 9 YDR134C - - - - - - 4068 BY4741 04_4 11 E 10 YDR135C YCF1 - Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR transmembrane transport, response to chemical stimulus ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 4069 BY4741 04_4 11 E 11 YDR136C VPS61 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect protein targeting molecular_function cellular_component 4070 BY4741 04_4 11 E 12 YDR137W RGP1 - Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p endosome transport enzyme regulator activity membrane, endomembrane system, cytoplasm, Golgi apparatus 4071 BY4741 04_4 11 F 1 YPR021C AGC1 - Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 7400 BY4741 00_18 "Hyperrecombination protein related to Top 1 p -- growth on -met, growth on -lys, mates poorly" 11 F 2 YDR139C RUB1 - Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) protein modification by small protein conjugation or removal other cytoplasm 4073 BY4741 04_4 11 F 3 YDR140W MTQ2 - S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC regulation of translation, protein alkylation methyltransferase activity nucleus, cytoplasm 4074 BY4741 04_4 11 F 4 YDR142C PEX7 PEB1|PAS7 Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) protein targeting, peroxisome organization, protein complex biogenesis other peroxisome, cytoplasm 4076 BY4741 04_4 11 F 5 YDR143C SAN1 - Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process, response to chemical stimulus ligase activity nucleus, cytoplasm 4077 BY4741 04_4 11 F 6 YDR144C MKC7 YPS2 GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p cell wall organization or biogenesis peptidase activity cell wall 4078 BY4741 04_4 11 F 7 YDR146C SWI5 - Transcription factor that activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase mitotic cell cycle, transcription from RNA polymerase II promoter DNA binding nucleus, cytoplasm 4080 BY4741 04_4 "Transcription factor -- growth on -met, growth on -lys, mates poorly" 11 F 8 YDR338C - - Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily transmembrane transport transmembrane transporter activity membrane 4177 BY4741 04_7 11 F 9 YDR340W - - Putative protein of unknown function biological_process molecular_function cellular_component 4179 BY4741 04_7 11 F 10 YDR344C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4181 BY4741 04_7 11 F 11 YDR345C HXT3 - Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 4182 BY4741 04_7 11 F 12 YDR346C SVF1 SGI1 Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance response to oxidative stress, response to chemical stimulus molecular_function nucleus, cytoplasm 4183 BY4741 04_7 11 G 1 YDR347W MRP1 - Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4184 BY4741 04_7 11 G 2 YDR348C PAL1 - Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein biological_process molecular_function cellular bud, cell cortex, site of polarized growth, cytoplasm 4185 BY4741 04_7 11 G 3 YDR349C YPS7 - Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum cell wall organization or biogenesis peptidase activity endoplasmic reticulum, cytoplasm 4186 BY4741 04_7 11 G 4 YDR350C ATP22 TCM10 Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 4187 BY4741 04_7 11 G 5 YDR351W SBE2 - Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth cell wall organization or biogenesis molecular_function cytoplasm, Golgi apparatus 4188 BY4741 04_7 11 G 6 YDR352W - - Putative protein of unknown function biological_process molecular_function membrane, vacuole, cytoplasm 4189 BY4741 04_7 11 G 7 YDR354W TRP4 - Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis cellular amino acid metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm 4191 BY4741 04_7 11 G 8 YDR357C CNL1 BLC1 Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 4194 BY4741 04_7 11 G 9 YDR358W GGA1 - Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Golgi vesicle transport, protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process small conjugating protein binding cytoplasm, Golgi apparatus 4195 BY4741 04_7 11 G 10 YDR359C EAF1 VID21 Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 response to DNA damage stimulus, DNA repair, chromatin organization, protein complex biogenesis molecular_function nucleus 4196 BY4741 04_7 11 G 11 YDR360W OPI7 - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. biological_process molecular_function cellular_component 4197 BY4741 04_7 11 G 12 YDR363W ESC2 - Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member mitotic cell cycle, DNA repair, organelle fission, chromosome segregation, response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus 4200 BY4741 04_7 11 H 1 YDR364C CDC40 SLU4|SLT15|PRP17 Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats mitotic cell cycle, RNA splicing, mRNA processing other nucleus 4201 BY4741 04_7 11 H 3 YDR368W YPR1 - NADPH-dependent aldo-keto reductase, reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 4204 BY4741 04_7 11 H 4 YDR369C XRS2 - Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling meiotic cell cycle, DNA repair, telomere organization, sporulation, response to DNA damage stimulus, DNA recombination protein binding, bridging, DNA binding nucleus 4205 BY4741 04_7 11 H 5 YDR370C - - Putative protein of unknown function biological_process molecular_function cytoplasm 4206 BY4741 04_7 11 H 6 YDR371W CTS2 - Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect sporulation molecular_function cytoplasm 4207 BY4741 04_7 11 H 7 YDR372C VPS74 MNN3|API1 Protein required for Golgi localization of glycosyltransferases; binds the cytosolic domains of Golgi glycosyltransferases; binding to PtdIns4P required for Golgi targeting and function; tetramer formation required for function response to chemical stimulus, Golgi vesicle transport, signaling lipid binding, enzyme binding nucleus, membrane, Golgi apparatus, cytoplasm 4208 BY4741 04_7 11 H 8 YDR374C - - Putative protein of unknown function biological_process molecular_function cellular_component 4210 BY4741 04_7 11 H 9 YDR375C BCS1 - Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases mitochondrion organization, protein complex biogenesis protein transporter activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4211 BY4741 04_7 11 H 10 YDR377W ATP17 - Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4213 BY4741 04_7 11 H 11 YDR378C LSM6 - Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA RNA splicing, rRNA processing, ribosomal small subunit biogenesis, RNA catabolic process, mRNA processing RNA binding nucleus, nucleolus, cytoplasm 4214 BY4741 04_7 11 H 12 YDR379W RGA2 - GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p invasive growth in response to glucose limitation, cell budding, cytoskeleton organization, response to chemical stimulus, conjugation, pseudohyphal growth, signaling enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 4215 BY4741 04_7 12 A 1 YDR380W ARO10 - Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway cellular amino acid metabolic process lyase activity cytoplasm 4216 BY4741 04_7 12 A 2 YDR382W RPP2B YP2beta|P2B|L45|YPA1|RPL45 Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4218 BY4741 04_7 12 A 3 YDR383C NKP1 - Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) biological_process molecular_function chromosome 4219 BY4741 04_7 12 A 4 YDR384C ATO3 - Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 4220 BY4741 04_7 12 A 5 YDR385W EFT2 - Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin regulation of translation, translational elongation RNA binding, translation factor activity, nucleic acid binding ribosome, cytoplasm 4221 BY4741 04_7 12 A 6 YDR386W MUS81 SLX3 Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination, chromosome segregation nuclease activity nucleus 4222 BY4741 04_7 12 A 7 YDR387C - - Putative transporter, member of the sugar porter family; YDR387C is not an essential gene transmembrane transport transmembrane transporter activity membrane 4223 BY4741 04_7 12 A 8 YDR388W RVS167 - Actin-associated protein with roles in endocytosis and exocytosis; interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin membrane invagination, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm 4224 BY4741 04_7 12 A 9 YDR389W SAC7 - GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate cytoskeleton organization, signaling enzyme regulator activity other 4225 BY4741 04_7 12 A 10 YDR391C - - Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 4227 BY4741 04_7 12 A 11 YDR392W SPT3 - Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters peptidyl-amino acid modification, protein acylation, invasive growth in response to glucose limitation, chromatin organization, sporulation, histone modification, conjugation, pseudohyphal growth, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 4228 BY4741 04_7 12 B 1 YDR393W SHE9 MDM33 Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4229 BY4741 04_7 12 B 2 YDR395W SXM1 KAP108 Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 nucleobase-containing compound transport, nuclear transport protein transporter activity nucleus, membrane, cytoplasm 4231 BY4741 04_7 12 B 3 YDR399W HPT1 HGPRTase|HPRT|BRA6 Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the formation of both inosine monophosphate and guanosine monophosphate; mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm 4235 BY4741 04_7 12 B 4 YDR400W URH1 - Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways cofactor metabolic process, nucleobase-containing small molecule metabolic process, proteolysis involved in cellular protein catabolic process hydrolase activity, acting on glycosyl bonds nucleus, cytoplasm 4236 BY4741 04_7 12 B 5 YDR401W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4237 BY4741 04_7 12 B 6 YDR402C DIT2 CYP56 N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s sporulation, cell wall organization or biogenesis oxidoreductase activity cellular_component 4238 BY4741 04_7 12 B 7 YDR403W DIT1 - Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure sporulation, cell wall organization or biogenesis other cellular_component 4239 BY4741 04_7 12 B 8 YDR405W MRP20 YmL41|MRPL41 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4241 BY4741 04_7 12 B 9 YDR406W PDR15 - Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element response to chemical stimulus ATPase activity, transmembrane transporter activity membrane 4242 BY4741 04_7 12 B 10 YDR408C ADE8 - Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process other nucleus, cytoplasm 4244 BY4741 04_7 12 B 11 YDR409W SIZ1 ULL1 SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring protein modification by small protein conjugation or removal, chromosome segregation ligase activity nucleus, cell cortex, cytoskeleton, chromosome, cytoplasm 4245 BY4741 04_7 12 B 12 YDR410C STE14 - Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane protein alkylation, protein maturation methyltransferase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4246 BY4741 04_7 Sterile [expected phenotype] 12 C 1 YDR411C DFM1 - Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p proteolysis involved in cellular protein catabolic process, response to chemical stimulus, signaling molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4247 BY4741 04_7 12 C 2 YDR414C ERD1 LDB2 Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) protein glycosylation, carbohydrate metabolic process molecular_function membrane 4250 BY4741 04_7 12 C 3 YDR415C - - Putative protein of unknown function biological_process molecular_function cellular_component 4251 BY4741 04_7 12 C 4 YDR418W RPL12B YL23|L15B|L12B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 4254 BY4741 04_7 12 C 5 YDR419W RAD30 DBH1 DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; mutations in human pol eta are responsible for XPV response to DNA damage stimulus, DNA repair nucleotidyltransferase activity chromosome 4255 BY4741 04_7 12 C 6 YDR420W HKR1 - Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection carbohydrate metabolic process, cytokinesis, cell wall organization or biogenesis, signaling, response to osmotic stress signal transducer activity membrane, site of polarized growth, plasma membrane 4256 BY4741 04_7 12 C 7 YDR421W ARO80 - Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 4257 BY4741 04_7 12 C 8 YDR422C SIP1 - Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions protein phosphorylation, signaling, protein complex biogenesis kinase activity vacuole, cytoplasm 4258 BY4741 04_7 12 C 9 YDR423C CAD1 YAP2 AP-1-like basic leucine zipper (bZIP) transcriptional activator involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4259 BY4741 04_7 12 C 10 YDR425W SNX41 - Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p endosome transport lipid binding cytoplasm 4261 BY4741 04_7 12 C 11 YDR426C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 biological_process molecular_function cellular_component 4262 BY4741 04_7 12 C 12 YDR428C BNA7 - Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component 4264 BY4741 04_7 12 D 1 YDR430C CYM1 MOP112 Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis involved in cellular protein catabolic process peptidase activity mitochondrial envelope, mitochondrion, cytoplasm 4266 BY4741 04_7 12 D 2 YDR431W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4267 BY4741 04_7 12 D 3 YDR432W NPL3 NAB1|NOP3|MTS1|MTR13 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm RNA splicing, nucleobase-containing compound transport, transcription termination, DNA-dependent, transcription elongation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport RNA binding, enzyme binding, mRNA binding nucleus, cytoplasm 4268 BY4741 04_7 12 D 4 YDR433W - KRE22 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data response to DNA damage stimulus molecular_function cellular_component 4269 BY4741 04_7 12 D 5 YDR435C PPM1 - Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits protein alkylation, protein complex biogenesis methyltransferase activity cellular_component 4271 BY4741 04_7 12 D 6 YDR436W PPZ2 - Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis phosphatase activity cellular_component 4272 BY4741 04_7 12 D 7 YEL001C IRC22 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endoplasmic reticulum, cytoplasm 241 BY4741 05_2 12 D 8 YEL003W GIM4 PFD2 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it protein complex biogenesis cytoskeletal protein binding cytoplasm 243 BY4741 05_2 12 D 9 YEL004W YEA4 - Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER carbohydrate metabolic process, transmembrane transport, carbohydrate transport, cell wall organization or biogenesis transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 244 BY4741 05_2 "Similar to K. lactis golgi uridine diphosphate-N-acetylglucosamine transporter -- growth on -met, no growth on -lys, no growth on drop-in media, confirmed alpha -- CORRECT STRAIN CAN BE FOUND IN PLATE 121 D6" 12 D 10 YEL005C VAB2 VAB31 Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter biological_process molecular_function cytoplasm 245 BY4741 05_2 12 D 11 YEL006W YEA6 NDT2 Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog other other membrane, mitochondrial envelope, mitochondrion, cytoplasm 246 BY4741 05_2 12 D 12 YEL007W MIT1 TOS9 Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 biological_process molecular_function nucleus, cytoplasm 247 BY4741 05_2 12 E 1 YEL008W - - Hypothetical protein predicted to be involved in metabolism biological_process molecular_function cellular_component 248 BY4741 05_2 12 E 2 YEL009C GCN4 AAS101|ARG9|AAS3 Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, response to chemical stimulus, protein complex biogenesis protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus 249 BY4741 05_2 12 E 3 YEL010W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 250 BY4741 05_2 12 E 4 YEL013W VAC8 YEB3 Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions vacuole organization, protein targeting, organelle assembly, vesicle organization, membrane fusion, membrane invagination, organelle inheritance, response to starvation other vacuole, membrane, cytoplasm 253 BY4741 05_2 12 E 5 YEL014C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 254 BY4741 05_2 12 E 6 YEL015W EDC3 LSM16|DCP3 Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies RNA catabolic process molecular_function cytoplasm 255 BY4741 05_2 12 E 7 YEL016C NPP2 - Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation nucleobase-containing small molecule metabolic process nuclease activity cellular_component 256 BY4741 05_2 12 E 8 YEL017C-A PMP2 - Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; nearly identical to PMP1 ion transport molecular_function membrane 257 BY4741 05_2 12 E 9 YEL017W GTT3 - Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery biological_process molecular_function cellular_component 258 BY4741 05_2 12 E 10 YPR083W MDM36 - Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission organelle fission, mitochondrion organization molecular_function mitochondrion, cytoplasm 7401 BY4741 00_18 12 E 11 YEL020C - - Hypothetical protein with low sequence identity to Pdc1p biological_process molecular_function cytoplasm 261 BY4741 05_2 12 E 12 YEL023C - - Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene biological_process molecular_function cellular_component 264 BY4741 05_2 12 F 1 YEL024W RIP1 - Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 265 BY4741 05_2 12 F 2 YEL025C - SRI1 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 266 BY4741 05_2 12 F 3 YEL027W VMA3 CUP5|GEF2|CLS7 Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis vacuole organization, protein targeting, ion transport, cellular ion homeostasis, membrane invagination, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm 268 BY4741 05_2 12 F 4 YEL028W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 269 BY4741 05_2 12 F 5 YEL030W ECM10 SSC3 Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations protein targeting, protein folding, mitochondrion organization molecular_function mitochondrion, cytoplasm 271 BY4741 05_2 12 F 6 YEL031W SPF1 PER9|PIO1|COD1 P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 transmembrane transport, cellular ion homeostasis transmembrane transporter activity, ATPase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 272 BY4741 05_2 12 F 7 YEL033W MTC7 - Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant biological_process molecular_function cellular_component 274 BY4741 05_2 12 F 8 YPR091C NVJ2 - Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments biological_process lipid binding membrane, ribosome, endoplasmic reticulum, cytoplasm 7403 BY4741 00_18 12 F 9 YEL037C RAD23 - Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover DNA repair, proteolysis involved in cellular protein catabolic process, response to DNA damage stimulus protein binding, bridging, hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, small conjugating protein binding, DNA binding nucleus, mitochondrion, cytoplasm 278 BY4741 05_2 12 F 10 YEL038W UTR4 - Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus cellular amino acid metabolic process phosphatase activity nucleus, cytoplasm 279 BY4741 05_2 12 F 11 YEL039C CYC7 iso-2-cytochrome c Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration generation of precursor metabolites and energy, cellular respiration other mitochondrial envelope, mitochondrion, cytoplasm 280 BY4741 05_2 12 F 12 YEL040W UTR2 CRH2 Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups cell cortex, membrane fraction, cytoskeleton, cell wall, cellular bud, site of polarized growth, cytoplasm 281 BY4741 05_2 12 G 1 YEL041W YEF1 - ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes cofactor metabolic process, nucleobase-containing small molecule metabolic process kinase activity cellular_component 282 BY4741 05_2 12 G 2 YEL042W GDA1 - Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate protein glycosylation, carbohydrate metabolic process other cytoplasm, Golgi apparatus 283 BY4741 05_2 12 G 3 YEL043W - - Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold biological_process molecular_function ribosome, endoplasmic reticulum, cytoplasm 284 BY4741 05_2 12 G 4 YEL045C - - Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress biological_process molecular_function membrane 286 BY4741 05_2 "Similar to cytochrome c oxidase III of T. brucei kinetoplast -- no growth on -met, super slow growth on -lys, super slow growth on drop in media" 12 G 5 YEL046C GLY1 - Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis cellular amino acid metabolic process lyase activity cytoplasm 287 BY4741 05_2 "L-threonine aldolase -- no growth on -met, super slow growth on -lys, super slow growth on drop in media" 12 G 6 YEL047C - FRDS1 Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies response to chemical stimulus oxidoreductase activity membrane fraction, ribosome, mitochondrion, cytoplasm 288 BY4741 05_2 12 G 7 YEL048C TCA17 - Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Golgi vesicle transport, endosome transport, protein complex biogenesis molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 289 BY4741 05_2 12 G 8 YEL049W PAU2 - Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component 290 BY4741 05_2 12 G 9 YEL050C RML2 - Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor cytoplasmic translation, lipid metabolic process structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 291 BY4741 05_2 12 G 10 YEL051W VMA8 - Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 292 BY4741 05_2 12 G 11 YEL052W AFG1 - Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain protein targeting, proteolysis involved in cellular protein catabolic process, peroxisome organization, response to oxidative stress, response to chemical stimulus molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 293 BY4741 05_2 12 G 12 YEL053C MAK10 NAA35 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible protein acylation transferase activity, transferring acyl groups cytoplasm 294 BY4741 05_2 12 H 1 YEL054C RPL12A YL23|L15A|L12A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 295 BY4741 05_2 12 H 3 YEL056W HAT2 - Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification histone binding, transferase activity, transferring acyl groups nucleus, cytoplasm 297 BY4741 05_2 12 H 4 YEL057C - - Protein of unknown function involved in telomere maintenance; target of UME6 regulation biological_process molecular_function cellular_component 298 BY4741 05_2 12 H 5 YEL059W HHY1 ENV6 Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking biological_process molecular_function membrane 301 BY4741 05_2 12 H 6 YEL060C PRB1 CVT1 Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p sporulation, response to starvation peptidase activity vacuole, cytoplasm 302 BY4741 05_2 12 H 7 YEL061C CIN8 SDS15|KSL2 Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation other nucleus, cytoskeleton, chromosome, mitochondrion, cytoplasm 303 BY4741 05_2 12 H 8 YEL062W NPR2 - Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline response to chemical stimulus, amino acid transport, response to starvation, signaling molecular_function vacuole, membrane, cytoplasm 304 BY4741 05_2 12 H 9 YEL063C CAN1 - Plasma membrane arginine permease, requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance transmembrane transport, amino acid transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 305 BY4741 05_2 12 H 10 YEL064C AVT2 - Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters other other membrane, endoplasmic reticulum, cytoplasm 306 BY4741 05_2 12 H 11 YEL065W SIT1 ARN3 Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, cytoplasm 307 BY4741 05_2 12 H 12 YEL066W HPA3 - D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro cellular amino acid metabolic process transferase activity, transferring acyl groups nucleus, cytoplasm 308 BY4741 05_2 13 A 1 YEL067C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 309 BY4741 05_2 13 A 2 YEL068C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 310 BY4741 05_2 13 A 3 YEL071W DLD3 - D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm other oxidoreductase activity cytoplasm 313 BY4741 05_2 13 A 4 YEL072W RMD6 - Protein required for sporulation biological_process molecular_function cellular_component 314 BY4741 05_2 Hypothetical protein -- mates like alpha PCR mating type alpha 13 A 5 YER001W MNN1 - Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family protein glycosylation, carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus 322 BY4741 05_2 13 A 6 YER002W NOP16 - Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 323 BY4741 05_2 13 A 7 YER004W FMP52 - Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 325 BY4741 05_2 13 A 8 YER005W YND1 YEJ5 Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity carbohydrate metabolic process, protein glycosylation other membrane fraction, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 326 BY4741 05_2 13 A 9 YER007C-A TMA20 RBF20 Protein of unknown function that associates with ribosomes and has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1 other RNA binding ribosome, cytoplasm 328 BY4741 05_2 13 A 10 YER007W PAC2 - Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl protein folding cytoskeletal protein binding cellular_component 329 BY4741 05_2 13 A 11 YER010C - - Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction biological_process molecular_function cellular_component 332 BY4741 05_2 13 A 12 YER011W TIR1 SRP1 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking other structural molecule activity cell wall 333 BY4741 05_2 13 B 2 YPR011C - - Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies other other membrane, mitochondrial envelope, mitochondrion, cytoplasm 7399 BY4741 00_18 13 B 3 YER017C AFG3 YTA10 Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes protein targeting, mitochondrion organization, transmembrane transport, protein maturation, protein complex biogenesis peptidase activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 148 BY4741 05_3 13 B 4 YER019W ISC1 - Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance lipid metabolic process, response to osmotic stress other mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 150 BY4741 05_3 13 B 5 YER019C-A SBH2 SEB2 Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p protein targeting enzyme regulator activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 151 BY4741 05_3 13 B 6 YER020W GPA2 SSP101 Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery sporulation, response to chemical stimulus, pseudohyphal growth, nucleobase-containing small molecule metabolic process, signaling GTPase activity membrane fraction, mitochondrion, membrane, cytoplasm, plasma membrane 152 BY4741 05_3 13 B 7 YER024W YAT2 - Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane cellular amino acid metabolic process transferase activity, transferring acyl groups cytoplasm 156 BY4741 05_3 13 B 8 YER030W CHZ1 - Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif chromatin organization histone binding nucleus 162 BY4741 05_3 13 B 9 YER032W FIR1 PIP1 Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate mRNA processing molecular_function cellular bud, site of polarized growth 164 BY4741 05_3 13 B 10 YER033C ZRG8 - Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency cell wall organization or biogenesis molecular_function cellular bud, site of polarized growth, mitochondrion, cytoplasm 165 BY4741 05_3 13 B 11 YER034W - - Putative protein of unknown function; non-essential gene; expression induced upon calcium shortage biological_process molecular_function nucleus, cytoplasm 166 BY4741 05_3 13 B 12 YER035W EDC2 - RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress RNA catabolic process, regulation of translation RNA binding, mRNA binding nucleus, nucleolus, cytoplasm 167 BY4741 05_3 13 C 1 YER038W-A - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria biological_process molecular_function mitochondrion, cytoplasm 171 BY4741 05_3 13 C 2 YER039C HVG1 YEM9 Protein of unknown function, has homology to Vrg4p biological_process molecular_function membrane 172 BY4741 05_3 13 C 3 YER040W GLN3 - Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 173 BY4741 05_3 13 C 4 YER041W YEN1 - Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p response to DNA damage stimulus, DNA repair nuclease activity nucleus, cytoplasm 174 BY4741 05_3 13 C 5 YER042W MXR1 MSRA Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 175 BY4741 05_3 13 C 6 YER044C-A MEI4 - Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic cell cycle molecular_function nucleus, chromosome 178 BY4741 05_3 13 C 7 YER045C ACA1 - Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, may regulate transcription of genes involved in utilization of non-optimal carbon sources transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 179 BY4741 05_3 13 C 8 YER046W-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 181 BY4741 05_3 13 C 9 YER047C SAP1 - Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system biological_process ATPase activity cytoplasm 182 BY4741 05_3 13 C 10 YER048C CAJ1 - Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly protein folding other nucleus 183 BY4741 05_3 13 C 11 YER049W TPA1 - Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate RNA catabolic process RNA binding, mRNA binding nucleus 184 BY4741 05_3 13 C 12 YER050C RSM18 - Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 185 BY4741 05_3 13 D 1 YER051W JHD1 KDM2|JHDM1 JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe chromatin organization, histone modification histone binding, oxidoreductase activity cellular_component 186 BY4741 05_3 13 D 2 YER052C HOM3 SIL4|BOR1 Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis cellular amino acid metabolic process kinase activity cytoplasm 187 BY4741 05_3 13 D 3 YER053C PIC2 - Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, cytoplasm 188 BY4741 05_3 13 D 4 YER054C GIP2 - Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p carbohydrate metabolic process, protein dephosphorylation, generation of precursor metabolites and energy enzyme regulator activity cytoplasm 189 BY4741 05_3 13 D 5 YER056C FCY2 BRA7 Purine-cytosine permease, mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 191 BY4741 05_3 13 D 6 YER056C-A RPL34A L34A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Bp and has similarity to rat L34 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 192 BY4741 05_3 13 D 7 YER057C HMF1 HIG1 Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro biological_process molecular_function nucleus, cytoplasm 193 BY4741 05_3 13 D 8 YER058W PET117 - Protein required for assembly of cytochrome c oxidase mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 194 BY4741 05_3 13 D 9 YER059W PCL6 - Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other 195 BY4741 05_3 13 D 10 YER060W FCY21 - Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport transmembrane transporter activity membrane 196 BY4741 05_3 13 D 11 YER060W-A FCY22 - Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport transmembrane transporter activity membrane 197 BY4741 05_3 "similar to Fcy2p -- growth on -met, no growth on -lys, no growth on drop-in media" 13 D 12 YER061C CEM1 - Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration lipid metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm 198 BY4741 05_3 13 E 1 YER062C HOR2 GPP2 One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition carbohydrate metabolic process, generation of precursor metabolites and energy, response to osmotic stress phosphatase activity nucleus, cytoplasm 199 BY4741 05_3 13 E 2 YER065C ICL1 - Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose carbohydrate metabolic process lyase activity cellular_component 202 BY4741 05_3 13 E 3 YER066C-A - - Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W biological_process molecular_function cellular_component 204 BY4741 05_3 13 E 4 YER067W RGI1 - Protein of unknown function involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion generation of precursor metabolites and energy molecular_function nucleus, membrane fraction, cytoplasm 205 BY4741 05_3 13 E 5 YER067C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W biological_process molecular_function cellular_component 206 BY4741 05_3 13 E 6 YER068W MOT2 SIG1|NOT4 Subunit of the CCR4-NOT complex, which has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p RNA catabolic process, conjugation, protein modification by small protein conjugation or removal, transcription elongation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter ligase activity, DNA binding nucleus, cytoplasm 207 BY4741 05_3 13 E 7 YER068C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 208 BY4741 05_3 13 E 8 YER069W ARG5,6 - Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p cellular amino acid metabolic process kinase activity, oxidoreductase activity mitochondrion, cytoplasm 209 BY4741 05_3 13 E 9 YPR118W MRI1 - 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway cellular amino acid metabolic process isomerase activity nucleus, cytoplasm 7405 BY4741 00_18 13 E 10 YER071C TDA2 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function site of polarized growth, cytoplasm 211 BY4741 05_3 13 E 11 YER072W VTC1 PHM4|NRF1 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity vacuole organization, membrane fusion, membrane invagination RNA binding, mRNA binding vacuole, membrane, endoplasmic reticulum, cytoplasm 212 BY4741 05_3 13 E 12 YER073W ALD5 - Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed other oxidoreductase activity mitochondrion, cytoplasm 213 BY4741 05_3 13 F 1 YER074W RPS24A S24A|RPS24EA Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 214 BY4741 05_3 13 F 2 YER075C PTP3 - Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm protein phosphorylation, sporulation, protein dephosphorylation, response to chemical stimulus, regulation of protein modification process, conjugation, signaling, response to osmotic stress phosphatase activity nucleus, cytoplasm 215 BY4741 05_3 13 F 3 YER079W - - Putative protein of unknown function biological_process molecular_function nucleus, cytoplasm 219 BY4741 05_3 13 F 4 YER080W AIM9 FMP29 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 220 BY4741 05_3 13 F 5 YER081W SER3 - 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser33p cellular amino acid metabolic process oxidoreductase activity cytoplasm 221 BY4741 05_3 13 F 6 YER083C GET2 RMD7|HUR2 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Golgi vesicle transport other membrane, endoplasmic reticulum, cytoplasm 223 BY4741 05_3 13 F 7 YER084W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 224 BY4741 05_3 13 F 8 YER085C - - Putative protein of unknown function biological_process molecular_function cellular_component 225 BY4741 05_3 13 F 9 YER086W ILV1 ISO1 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 226 BY4741 05_3 13 F 10 YER087C-A - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase biological_process molecular_function cellular_component 228 BY4741 05_3 13 F 11 YGR123C PPT1 - Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth; computational analyses suggest roles in phosphate metabolism and rRNA processing protein dephosphorylation phosphatase activity nucleus, cytoplasm 4753 BY4741 07_5 13 F 12 YGR124W ASN2 - Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway cellular amino acid metabolic process ligase activity cytoplasm 4754 BY4741 07_5 13 G 1 YGR125W - - Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function membrane, vacuole, cytoplasm 4755 BY4741 07_5 13 G 2 YGR126W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm 4756 BY4741 07_5 13 G 3 YGR127W - - Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component 4757 BY4741 07_5 13 G 4 YGR129W SYF2 NTC31 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest RNA splicing, mRNA processing other nucleus 4759 BY4741 07_5 13 G 5 YGR130C - - Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) biological_process molecular_function membrane, cell cortex, cytoplasm 4760 BY4741 07_5 13 G 6 YGR131W FHN1 - Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain other molecular_function membrane 4761 BY4741 07_5 13 G 7 YGR132C PHB1 - Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation protein folding, organelle inheritance, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4762 BY4741 07_5 13 G 8 YGR133W PEX4 UBC10|PAS2 Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis protein targeting, protein modification by small protein conjugation or removal, peroxisome organization, signaling ligase activity peroxisome, cytoplasm 4763 BY4741 07_5 13 G 9 YGR135W PRE9 - Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, endomembrane system, mitochondrion, membrane, cytoplasm 4765 BY4741 07_5 13 G 10 YGR136W LSB1 - Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization biological_process molecular_function nucleus, cytoplasm 4766 BY4741 07_5 13 G 11 YGR137W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4767 BY4741 07_5 13 G 12 YGR138C TPO2 - Polyamine transport protein specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; member of the major facilitator superfamily other transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 4768 BY4741 07_5 13 H 1 YGR139W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4769 BY4741 07_5 13 H 3 YGR141W VPS62 - Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins protein targeting molecular_function cellular_component 4771 BY4741 07_5 13 H 4 YGR142W BTN2 - v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase endosome transport, amino acid transport other cytoplasm 4772 BY4741 07_5 13 H 5 YGR143W SKN1 - Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p carbohydrate metabolic process, lipid metabolic process, cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds membrane 4773 BY4741 07_5 13 H 6 YGR144W THI4 MOL1|ESP35 Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents mitochondrion organization, vitamin metabolic process ion binding cytoplasm 4774 BY4741 07_5 13 H 7 YGR146C ECL1 - Protein of unknown function, affects chronological lifespan; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function cellular_component 4776 BY4741 07_5 13 H 8 YGR148C RPL24B rp29|YL21|L30B|L24B|RPL30B Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4778 BY4741 07_5 13 H 9 YGR149W - - Putative protein of unknown function; predicted to be an integal membrane protein biological_process molecular_function membrane 4779 BY4741 07_5 13 H 10 YGR150C CCM1 DMR1|RRG2 Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport mitochondrion organization RNA binding, rRNA binding mitochondrion, cytoplasm 4780 BY4741 07_5 13 H 11 YGR151C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C biological_process molecular_function cellular_component 4781 BY4741 07_5 13 H 12 YGR152C RSR1 BUD1 GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases cell budding, cytokinesis, signaling GTPase activity, signal transducer activity cellular bud, membrane, site of polarized growth, plasma membrane 4782 BY4741 07_5 14 A 1 YGR153W - TOS10 Putative protein of unknown function biological_process molecular_function cellular_component 4783 BY4741 07_5 14 A 2 YGR154C GTO1 - Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups peroxisome, cytoplasm 4784 BY4741 07_5 14 A 3 YGR157W CHO2 PEM1 Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis lipid metabolic process methyltransferase activity membrane, membrane fraction 4787 BY4741 07_5 14 A 4 YGR159C NSR1 SHE5 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, DNA binding nucleus, mitochondrion, nucleolus, cytoplasm 4789 BY4741 07_5 14 A 5 YGR160W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4790 BY4741 07_5 14 A 6 YGR161C RTS3 - Putative component of the protein phosphatase type 2A complex biological_process molecular_function nucleus, cytoplasm 4791 BY4741 07_5 14 A 7 YGR163W GTR2 - Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD membrane invagination, transcription from RNA polymerase II promoter other nucleus, chromosome, vacuole, membrane, cytoplasm 4793 BY4741 07_5 14 A 8 YGR164W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4794 BY4741 07_5 14 A 9 YGR165W MRPS35 - Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4795 BY4741 07_5 14 A 10 YGR166W TRS65 KRE11 Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Golgi vesicle transport, protein complex biogenesis enzyme regulator activity cytoplasm, Golgi apparatus 4796 BY4741 07_5 14 A 11 YOL153C - - - - - - 7133 BY4741 00_15 14 A 12 YGR168C - - Putative protein of unknown function; YGR168C is not an essential gene biological_process molecular_function membrane 4798 BY4741 07_5 "Hypothetical protein -- growth on -met, no growth on -lys, no growth on drop-in media" 14 B 1 YGR169C PUS6 - tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability tRNA processing, RNA modification isomerase activity mitochondrion, cytoplasm 4799 BY4741 07_5 14 B 3 YGR170W PSD2 - Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine lipid metabolic process lyase activity cytoplasm 4800 BY4741 07_5 14 B 4 YGR171C MSM1 mitochondrial methionyl-tRNA synthetase Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 4801 BY4741 07_5 14 B 5 YGR173W RBG2 GIR1 Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein cytoplasmic translation other cytoplasm 4803 BY4741 07_5 14 B 6 YGR174C CBP4 - Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4804 BY4741 07_5 14 B 7 YGR176W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4806 BY4741 07_5 14 B 8 YGR177C ATF2 - Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking lipid metabolic process, response to chemical stimulus transferase activity, transferring acyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4807 BY4741 07_5 14 B 9 YGR178C PBP1 MRS16 Component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation regulation of translation, mRNA processing molecular_function nucleus, mitochondrion, cytoplasm 4808 BY4741 07_5 14 B 10 YGR180C RNR4 PSO3|CRT3 Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity nucleus, cytoplasm 4810 BY4741 07_5 14 B 11 YGR181W TIM13 - Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein targeting, mitochondrion organization protein transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4811 BY4741 07_5 14 B 12 YGR182C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W biological_process molecular_function cellular_component 4812 BY4741 07_5 14 C 1 YGR183C QCR9 UCR9 Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4813 BY4741 07_5 14 C 2 YGR184C UBR1 PTR1 E3 ubiquitin ligase (N-recognin), forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) protein modification by small protein conjugation or removal, regulation of transport, proteolysis involved in cellular protein catabolic process, response to chemical stimulus ligase activity cytoplasm 4814 BY4741 07_5 14 C 3 YGR187C HGH1 - Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16) biological_process molecular_function cytoplasm 4817 BY4741 07_5 14 C 4 YGR189C CRH1 - Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups cell wall 4819 BY4741 07_5 14 C 5 YGR192C TDH3 GAPDH|GPD|SSS2|HSP36|HSP35|GLD1 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall carbohydrate metabolic process, generation of precursor metabolites and energy oxidoreductase activity cell wall, membrane fraction, mitochondrion, cytoplasm 4822 BY4741 07_5 14 C 6 YGR193C PDX1 - Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core cofactor metabolic process structural molecule activity mitochondrion, cytoplasm 4823 BY4741 07_5 14 C 7 YGR194C XKS1 - Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains carbohydrate metabolic process kinase activity cytoplasm 4824 BY4741 07_5 14 C 8 YGR196C FYV8 - Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cytoplasm 4826 BY4741 07_5 14 C 9 YGR197C SNG1 - Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance nucleobase-containing compound transport molecular_function membrane, plasma membrane 4827 BY4741 07_5 14 C 10 YGR199W PMT6 - Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 4829 BY4741 07_5 14 C 11 YGR200C ELP2 KTI3|TOT2 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin tRNA processing, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, RNA modification molecular_function nucleus, cytoplasm 4830 BY4741 07_5 14 C 12 YGR202C PCT1 CCT1|BSR2 Cholinephosphate cytidylyltransferase, also known as CTP:phosphocholine cytidylyltransferase, rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding lipid metabolic process nucleotidyltransferase activity nucleus, endomembrane system, cytoplasm, Golgi apparatus 4832 BY4741 07_5 14 D 1 YGR203W YCH1 - Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus other phosphatase activity nucleus, cytoplasm 4833 BY4741 07_5 14 D 2 YGR205W TDA10 - ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele biological_process other nucleus, cytoplasm 4835 BY4741 07_5 14 D 3 YGR206W MVB12 - ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin protein targeting, endosome transport, protein complex biogenesis small conjugating protein binding membrane, cytoplasm 4836 BY4741 07_5 14 D 4 YGR207C CIR1 - Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response biological_process molecular_function mitochondrion, cytoplasm 4837 BY4741 07_5 14 D 5 YGR208W SER2 - Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source cellular amino acid metabolic process phosphatase activity nucleus, cytoplasm 4838 BY4741 07_5 14 D 6 YGR209C TRX2 LMA1 Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance vacuole organization, DNA replication, response to oxidative stress, response to chemical stimulus, Golgi vesicle transport, membrane fusion, organelle inheritance, regulation of DNA metabolic process oxidoreductase activity vacuole, cytoplasm 4839 BY4741 07_5 14 D 7 YGR212W SLI1 - N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin response to chemical stimulus transferase activity, transferring acyl groups nucleus, membrane, endomembrane system, plasma membrane 4842 BY4741 07_5 14 D 8 YGR213C RTA1 - Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein biological_process molecular_function membrane, plasma membrane 4843 BY4741 07_5 14 D 9 YGR214W RPS0A S0A|YST1|NAB1A|NAB1 Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal cytoplasmic translation, rRNA processing, organelle assembly, nucleobase-containing compound transport, ribosomal small subunit biogenesis, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 4844 BY4741 07_5 14 D 10 YGR215W RSM27 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4845 BY4741 07_5 14 D 11 YGR217W CCH1 - Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together ion transport transmembrane transporter activity membrane, plasma membrane 4847 BY4741 07_5 14 D 12 YHL047C ARN2 TAF1 Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C cofactor metabolic process, cellular ion homeostasis, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, plasma membrane, cytoplasm 916 BY4741 08_1 14 E 1 YHL046C PAU13 - Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock biological_process molecular_function cellular_component 917 BY4741 08_1 14 E 2 YHL045W - - Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component 918 BY4741 08_1 14 E 3 YHL044W - - Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern biological_process molecular_function membrane, plasma membrane 919 BY4741 08_1 14 E 4 YHL043W ECM34 - Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins cell wall organization or biogenesis molecular_function cellular_component 920 BY4741 08_1 14 E 5 YHL042W - - Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function cellular_component 921 BY4741 08_1 14 E 6 YHL041W - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data biological_process molecular_function cellular_component 922 BY4741 08_1 14 E 7 YHL040C ARN1 - Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores cellular ion homeostasis, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, plasma membrane, cytoplasm 923 BY4741 08_1 14 E 8 YHL038C CBP2 - Mitochondrial protein required for splicing of the group I intron aI5 of the COB pre-mRNA, binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene RNA splicing RNA binding mitochondrion, cytoplasm 925 BY4741 08_1 14 E 9 YHL037C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 926 BY4741 08_1 14 E 10 YHL036W MUP3 - Low affinity methionine permease, similar to Mup1p amino acid transport transmembrane transporter activity membrane 927 BY4741 08_1 14 E 11 YHL035C VMR1 - Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions response to chemical stimulus transmembrane transporter activity, ATPase activity vacuole, ribosome, mitochondrion, membrane, cytoplasm 928 BY4741 08_1 14 E 12 YHL034C SBP1 SSB1|SSBR1 Putative RNA binding protein; involved in translational repression and found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 regulation of translation RNA binding nucleus, nucleolus, cytoplasm 929 BY4741 08_1 14 F 1 YHL033C RPL8A rp6|YL5|L8A|L4A|MAK7 Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 930 BY4741 08_1 14 F 2 YHL032C GUT1 - Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p carbohydrate metabolic process kinase activity mitochondrion, cytoplasm 931 BY4741 08_1 14 F 3 YHL031C GOS1 - v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 membrane fusion, Golgi vesicle transport, organelle fusion, vesicle organization other membrane, cytoplasm, Golgi apparatus 932 BY4741 08_1 14 F 4 YHL030W ECM29 - Scaffold protein that assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats protein complex biogenesis structural molecule activity nucleus, cytoplasm 933 BY4741 08_1 "Cell wall biogenesis & architecture -- growth on -met, no growth on -lys, no growth on drop-in media -- APPEARS ALPHA " 14 F 5 YHL029C OCA5 - Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm 934 BY4741 08_1 14 F 6 YHL028W WSC4 YFW1|YHC8 ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 response to heat, protein targeting, transmembrane transport signal transducer activity membrane fraction, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 935 BY4741 08_1 14 F 7 YHL027W RIM101 RIM1 Transcriptional repressor involved in response to pH and in cell wall construction; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by proteolytic processing; similar to A. nidulans PacC meiotic cell cycle, sporulation, cytokinesis, cell wall organization or biogenesis, transcription from RNA polymerase II promoter, response to chemical stimulus DNA binding nucleus 936 BY4741 08_1 14 F 8 YHL026C - - Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) biological_process molecular_function cellular_component 937 BY4741 08_1 14 F 9 YHL023C NPR3 RMD11 Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair meiotic cell cycle, response to starvation, signaling molecular_function membrane, vacuole, cytoplasm 940 BY4741 08_1 14 F 10 YHL022C SPO11 - Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation meiotic cell cycle nuclease activity nucleus, chromosome 941 BY4741 08_1 14 F 11 YHL021C AIM17 FMP12 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 942 BY4741 08_1 14 F 12 YHL020C OPI1 - Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance response to chemical stimulus, lipid metabolic process, transcription from RNA polymerase II promoter, signaling protein binding transcription factor activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 943 BY4741 08_1 14 G 1 YHL019C APM2 - Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 944 BY4741 08_1 14 G 2 YHL017W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles biological_process molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm 946 BY4741 08_1 14 G 3 YHL016C DUR3 - Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway other transmembrane transporter activity membrane, plasma membrane 947 BY4741 08_1 14 G 4 YHL014C YLF2 YLF1 Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other mitochondrion, cytoplasm 949 BY4741 08_1 14 G 5 YHL013C OTU2 - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization biological_process molecular_function ribosome, cytoplasm 950 BY4741 08_1 14 G 6 YHL012W - - Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase biological_process molecular_function cellular_component 951 BY4741 08_1 14 G 7 YHL010C ETP1 BRP2 Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences response to chemical stimulus small conjugating protein binding cellular_component 953 BY4741 08_1 14 G 8 YHL009C YAP3 - Basic leucine zipper (bZIP) transcription factor transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 954 BY4741 08_1 14 G 9 YHL008C - - Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole ion transport other membrane, vacuole, cytoplasm 955 BY4741 08_1 14 G 10 YHL007C STE20 - Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p vacuole organization, response to heat, mitotic cell cycle, transmembrane transport, response to chemical stimulus, lipid transport, conjugation, response to osmotic stress, invasive growth in response to glucose limitation, protein phosphorylation, cytokinesis, regulation of protein modification process, organelle inheritance, pseudohyphal growth, signaling, regulation of cell cycle signal transducer activity, kinase activity nucleus, membrane fraction, site of polarized growth 956 BY4741 08_1 Sterile [expected phenotype] 14 G 11 YHL006C SHU1 - Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function response to DNA damage stimulus, DNA repair, DNA recombination molecular_function nucleus, nucleolus 957 BY4741 08_1 14 G 12 YHL005C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W biological_process molecular_function cellular_component 959 BY4741 08_1 "Hypothetical protein -- growth on -met, no growth on -lys, no growth on drop-in media, petite -- APPEARS ALPHA" 14 H 1 YHL003C LAG1 - Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p lipid metabolic process transferase activity, transferring acyl groups membrane, endoplasmic reticulum, cytoplasm 960 BY4741 08_1 "Longevity assurance protein -- growth on -met, no growth on -lys, no growth on drop-in media -- APPEARS ALPHA" 14 H 3 YHR001W-A QCR10 - Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 964 BY4741 08_1 14 H 4 YHR011W DIA4 - Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth mitochondrial translation, invasive growth in response to glucose limitation, mitochondrion organization, cellular amino acid metabolic process, tRNA aminoacylation for protein translation, pseudohyphal growth ligase activity mitochondrion, cytoplasm 974 BY4741 08_1 14 H 5 YHR012W VPS29 VPT6|PEP11 Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval endosome transport protein transporter activity membrane, endomembrane system, cytoplasm 975 BY4741 08_1 14 H 6 YHR013C ARD1 NAA10 Subunit of N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups ribosome, cytoplasm 976 BY4741 08_1 14 H 7 YHR014W SPO13 - Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II meiotic cell cycle, regulation of organelle organization, regulation of cell cycle, chromosome segregation molecular_function nucleus, chromosome 977 BY4741 08_1 14 H 8 YHR015W MIP6 - Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export nucleobase-containing compound transport, nuclear transport RNA binding nucleus, membrane, endomembrane system 978 BY4741 08_1 14 H 9 YHR018C ARG4 - Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway cellular amino acid metabolic process lyase activity cytoplasm 981 BY4741 08_1 14 H 10 YHR022C - - Putative protein of unknown function; YHR022C is not an essential gene biological_process molecular_function cellular_component 985 BY4741 08_1 14 H 11 YHR029C YHI9 - Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production signaling, response to chemical stimulus molecular_function cellular_component 992 BY4741 08_1 14 H 12 YHR030C SLT2 SLK2|MPK1|BYC2 Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway transmembrane transport, regulation of transport, peroxisome organization, response to chemical stimulus, mRNA processing, cell wall organization or biogenesis, protein targeting, protein phosphorylation, cytokinesis, nuclear transport, signaling signal transducer activity, kinase activity nucleus, cellular bud, site of polarized growth, cytoplasm 993 BY4741 08_1 15 A 1 YHR031C RRM3 RTT104 DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p DNA replication, mitochondrion organization, regulation of DNA metabolic process helicase activity, ATPase activity nucleus, chromosome 994 BY4741 08_1 15 A 2 YHR034C PIH1 NOP17 Protein of unresolved function; may function in protein folding and/or rRNA processing, interacts with a chaperone (Hsp82p), two chromatin remodeling factors (Rvb1p, Rvb2p) and two rRNA processing factors (Rrp43p, Nop58p) protein folding, rRNA processing molecular_function nucleus, cytoplasm 997 BY4741 08_1 15 A 3 YHR035W - - Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component 998 BY4741 08_1 15 A 4 YHR037W PUT2 - Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 1000 BY4741 08_1 15 A 5 YHR038W RRF1 FIL1|KIM4 Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria mitochondrial translation, mitochondrion organization RNA binding, translation factor activity, nucleic acid binding mitochondrion, cytoplasm 1001 BY4741 08_1 15 A 6 YHR039C MSC7 - Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids meiotic cell cycle, DNA recombination molecular_function membrane, endoplasmic reticulum, cytoplasm 1002 BY4741 08_1 15 A 7 YHR043C DOG2 2-deoxyglucose-6-phosphate phosphatase 2-deoxyglucose-6-phosphate phosphatase, member of a family of low molecular weight phosphatases, similar to Dog1p, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process phosphatase activity nucleus, cytoplasm 1006 BY4741 08_1 15 A 8 YHR044C DOG1 2-deoxyglucose-6-phosphate phosphatase 2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p, member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified carbohydrate metabolic process phosphatase activity cellular_component 1007 BY4741 08_1 15 A 9 YHR046C INM1 - Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate carbohydrate metabolic process phosphatase activity nucleus, cytoplasm 1873 BY4741 08_2 15 A 10 YHR047C AAP1 AAP1' Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation carbohydrate metabolic process, generation of precursor metabolites and energy peptidase activity nucleus, cytoplasm 1874 BY4741 08_2 15 A 11 YHR048W YHK8 - Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles response to chemical stimulus molecular_function membrane 1875 BY4741 08_2 15 A 12 YHR049W FSH1 - Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process other nucleus, cytoplasm 1876 BY4741 08_2 15 B 1 YHR049C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1877 BY4741 08_2 15 B 2 YHR050W SMF2 - Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins cellular ion homeostasis, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, mitochondrion, cytoplasm 1878 BY4741 08_2 15 B 4 YHR051W COX6 - Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1879 BY4741 08_2 APPEARS TO BE ALPHA 15 B 5 YHR057C CPR2 CYP2 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway biological_process isomerase activity cellular_component 1885 BY4741 08_2 15 B 6 YHR060W VMA22 VPH6|CEV1 Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) cellular ion homeostasis, protein complex biogenesis unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1888 BY4741 08_2 15 B 7 YHR061C GIC1 - Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain mitotic cell cycle, cell budding, cytokinesis, signaling, regulation of cell cycle enzyme regulator activity, enzyme binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 1889 BY4741 08_2 15 B 8 YHR066W SSF1 - Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family ribosome assembly, conjugation, organelle assembly, ribosomal large subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus 1894 BY4741 08_2 15 B 9 YHR073W OSH3 - Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability invasive growth in response to glucose limitation, organelle fusion, lipid transport, membrane invagination, conjugation, exocytosis, pseudohyphal growth, nucleus organization, endocytosis lipid binding cell cortex, endoplasmic reticulum, cytoplasm 1901 BY4741 08_2 15 B 10 YHR075C PPE1 YmS2|MRPS2 Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 1903 BY4741 08_2 15 B 11 YHR076W PTC7 - Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope biological_process phosphatase activity nucleus, endomembrane system, mitochondrion, cytoplasm 1904 BY4741 08_2 15 B 12 YHR077C NMD2 SUP111|UPF2|SUA1|IFS1 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance RNA catabolic process molecular_function cytoplasm 1905 BY4741 08_2 15 C 1 YHR078W - - High osmolarity-regulated gene of unknown function biological_process molecular_function membrane 1906 BY4741 08_2 15 C 2 YHR079C IRE1 ERN1 Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress carbohydrate metabolic process, protein phosphorylation, response to chemical stimulus, cell wall organization or biogenesis, signaling kinase activity, unfolded protein binding, nuclease activity nucleus 1907 BY4741 08_2 15 C 3 YHR080C - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ribosome, mitochondrion, cytoplasm 1908 BY4741 08_2 15 C 4 YHR081W LRP1 RRP47|YC1D Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination RNA catabolic process, snoRNA processing, rRNA processing RNA binding, DNA binding nucleus 1909 BY4741 08_2 15 C 5 YHR082C KSP1 - Ser/thr protein kinase; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10 protein phosphorylation kinase activity nucleus 1910 BY4741 08_2 15 C 6 YHR086W NAM8 MUD15|MRE2 RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 1914 BY4741 08_2 15 C 7 YHR087W RTC3 - Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity other molecular_function nucleus, cytoplasm 1915 BY4741 08_2 15 C 8 YHR091C MSR1 mitochondrial arginyl-tRNA synthetase Mitochondrial arginyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 1919 BY4741 08_2 15 C 9 YHR092C HXT4 RAG1|LGT1 High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 1920 BY4741 08_2 15 C 10 YHR093W AHT1 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region biological_process molecular_function cellular_component 1921 BY4741 08_2 15 C 11 YHR094C HXT1 HOR4 Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 1922 BY4741 08_2 15 C 12 YHR095W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1923 BY4741 08_2 15 D 1 YHR096C HXT5 - Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 1924 BY4741 08_2 15 D 2 YHR097C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 1925 BY4741 08_2 15 D 3 YHR100C GEP4 - Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT carbohydrate metabolic process, lipid metabolic process phosphatase activity mitochondrion, cytoplasm 1928 BY4741 08_2 15 D 4 YHR103W SBE22 - Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth cell wall organization or biogenesis molecular_function cytoplasm 1931 BY4741 08_2 15 D 5 YHR104W GRE3 - Aldose reductase involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus RNA binding, oxidoreductase activity, mRNA binding nucleus, cytoplasm 1932 BY4741 08_2 15 D 6 YHR105W YPT35 - Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport biological_process lipid binding cytoplasm 1933 BY4741 08_2 15 D 7 YHR106W TRR2 - Mitochondrial thioredoxin reductase involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm 1934 BY4741 08_2 15 D 8 YHR108W GGA2 - Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin protein targeting, proteolysis involved in cellular protein catabolic process, Golgi vesicle transport, endosome transport lipid binding, small conjugating protein binding Golgi apparatus, cytoplasm 1936 BY4741 08_2 15 D 9 YHR109W CTM1 - Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth peptidyl-amino acid modification, protein alkylation methyltransferase activity cytoplasm 1937 BY4741 08_2 15 D 10 YHR110W ERP5 - Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport other molecular_function membrane 1938 BY4741 08_2 15 D 11 YHR111W UBA4 YHR1 Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p invasive growth in response to glucose limitation, cell budding, response to oxidative stress, response to chemical stimulus, tRNA processing, protein modification by small protein conjugation or removal, pseudohyphal growth, RNA modification nucleotidyltransferase activity cytoplasm 1939 BY4741 08_2 15 D 12 YHR112C - - Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway other lyase activity nucleus, cytoplasm 1940 BY4741 08_2 15 E 1 YHR113W APE4 - Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family other peptidase activity vacuole, ribosome, cytoplasm 1941 BY4741 08_2 15 E 2 YHR114W BZZ1 LSB7 SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins cytoskeleton organization, membrane invagination, response to osmotic stress, endocytosis molecular_function cell cortex, cytoskeleton, site of polarized growth, cytoplasm 1942 BY4741 08_2 15 E 3 YHR115C DMA1 CHF1 Protein involved in ubiquitin ligation; plays a role in regulating spindle position and orientation; functionally redundant with Dma2p; orthologous to human RNF8 protein, also has sequence similarity to human Chfr. mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis, protein modification by small protein conjugation or removal, regulation of cell cycle ligase activity cytoplasm 1943 BY4741 08_2 15 E 4 YHR116W COX23 - Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm 1944 BY4741 08_2 15 E 5 YHR117W TOM71 TOM72 Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins biological_process protein transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 1945 BY4741 08_2 15 E 6 YHR120W MSH1 - DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis response to DNA damage stimulus, DNA repair, mitochondrion organization DNA binding, ATPase activity mitochondrion, cytoplasm 1948 BY4741 08_2 15 E 7 YHR121W LSM12 - Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS other RNA binding nucleus, ribosome, cytoplasm 1949 BY4741 08_2 15 E 8 YHR123W EPT1 - sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability lipid metabolic process other membrane, cytoplasm, Golgi apparatus 1951 BY4741 08_2 15 E 9 YHR124W NDT80 - Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) meiotic cell cycle nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 1952 BY4741 08_2 15 E 10 YHR125W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1953 BY4741 08_2 15 E 11 YHR126C ANS1 - Putative protein of unknown function; transcription dependent upon Azf1p biological_process molecular_function cellular_component 1954 BY4741 08_2 15 E 12 YHR129C ARP1 ACT5 Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin mitotic cell cycle, cytoskeleton organization structural molecule activity cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 1957 BY4741 08_2 15 F 1 YHR130C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1958 BY4741 08_2 15 F 2 YHR132C ECM14 - Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly cell wall organization or biogenesis peptidase activity vacuole, cytoplasm 1960 BY4741 08_2 15 F 3 YHR133C NSG1 - Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins lipid metabolic process unfolded protein binding membrane, endoplasmic reticulum, cytoplasm 1961 BY4741 08_2 15 F 4 YHR134W WSS1 - Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response response to DNA damage stimulus, protein modification by small protein conjugation or removal molecular_function nucleus, endomembrane system 1962 BY4741 08_2 15 F 5 YHR135C YCK1 CKI2 Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p protein phosphorylation, cell morphogenesis, cytokinesis, response to chemical stimulus, membrane invagination, endocytosis kinase activity membrane fraction, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1963 BY4741 08_2 15 F 6 YHR136C SPL2 - Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm other enzyme regulator activity cytoplasm 1964 BY4741 08_2 15 F 7 YHR137W ARO9 - Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism cellular amino acid metabolic process other nucleus, cytoplasm 1965 BY4741 08_2 15 F 8 YHR138C - - Putative protein of unknown function; has similarity to Pbi2p; double null mutant lacking Pbi2p and Yhr138p exhibits highly fragmented vacuoles vacuole organization, membrane fusion enzyme regulator activity cellular_component 1966 BY4741 08_2 15 F 9 YHR139C SPS100 - Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin sporulation, cell wall organization or biogenesis molecular_function cell wall 1967 BY4741 08_2 15 F 10 YHR139C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1968 BY4741 08_2 15 F 11 YHR142W CHS7 - Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER carbohydrate metabolic process, Golgi vesicle transport, protein folding, cell wall organization or biogenesis unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2835 BY4741 08_3 15 F 12 YHR143W DSE2 - Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP pseudohyphal growth, cytokinesis hydrolase activity, acting on glycosyl bonds cell wall, extracellular region 2836 BY4741 08_3 15 G 1 YHR147C MRPL6 YmL16 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 2841 BY4741 08_3 15 G 2 YHR150W PEX28 - Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p peroxisome organization molecular_function peroxisome, membrane, cytoplasm 2844 BY4741 08_3 15 G 3 YHR151C MTC6 - Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component 2845 BY4741 08_3 15 G 4 YHR152W SPO12 - Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis mitotic cell cycle, meiotic cell cycle, regulation of cell cycle molecular_function nucleus, nucleolus 2846 BY4741 08_3 15 G 5 YHR153C SPO16 - Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation meiotic cell cycle, sporulation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 2847 BY4741 08_3 15 G 6 YHR154W RTT107 ESC4 Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition response to DNA damage stimulus, DNA repair, transposition molecular_function nucleus 2848 BY4741 08_3 15 G 7 YHR155W YSP1 - Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone biological_process molecular_function mitochondrion, cytoplasm 2849 BY4741 08_3 15 G 8 YHR156C LIN1 SNU40 Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication biological_process other nucleus, chromosome 2850 BY4741 08_3 15 G 9 YHR157W REC104 - Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiotic cell cycle, DNA recombination molecular_function nucleus, chromosome 2851 BY4741 08_3 15 G 10 YHR158C KEL1 - Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate mitotic cell cycle, cell morphogenesis, membrane fusion, conjugation, regulation of cell cycle molecular_function cellular bud, site of polarized growth, cytoplasm 2852 BY4741 08_3 15 G 11 YHR159W TDA11 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function cytoplasm 2853 BY4741 08_3 15 G 12 YHR160C PEX18 - Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p protein targeting, peroxisome organization other peroxisome, cytoplasm 2854 BY4741 08_3 15 H 1 YHR161C YAP1801 - Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1802p, member of the AP180 protein family membrane invagination, endocytosis other cell cortex, cytoskeleton, cytoplasm 2855 BY4741 08_3 15 H 3 YHR163W SOL3 - 6-phosphogluconolactonase, catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process other nucleus, cytoplasm 2857 BY4741 08_3 15 H 4 YHR167W THP2 - Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance DNA recombination, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, nucleobase-containing compound transport, nuclear transport other nucleus 2861 BY4741 08_3 15 H 5 YHR176W FMO1 - Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins protein folding oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2870 BY4741 08_3 15 H 6 YHR177W - GON3 Putative protein of unknown function; overexpression causes a cell cycle delay or arrest biological_process molecular_function cellular_component 2871 BY4741 08_3 15 H 7 YHR178W STB5 - Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro transcription from RNA polymerase II promoter, response to oxidative stress, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 2872 BY4741 08_3 15 H 8 YHR179W OYE2 Old Yellow Enzyme Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death other oxidoreductase activity nucleus, mitochondrion, cytoplasm 2873 BY4741 08_3 15 H 9 YHR182W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm biological_process molecular_function cytoplasm 2876 BY4741 08_3 15 H 10 YHR183W GND1 - 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress carbohydrate metabolic process, cofactor metabolic process, response to oxidative stress, response to chemical stimulus, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, cytoplasm 2877 BY4741 08_3 15 H 11 YHR184W SSP1 SPO3 Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis sporulation, cell wall organization or biogenesis, protein complex biogenesis molecular_function membrane 2878 BY4741 08_3 15 H 12 YHR189W PTH1 PTH One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium mitochondrial translation, mitochondrion organization other mitochondrion, cytoplasm 2883 BY4741 08_3 16 A 1 YHR195W NVJ1 VAB36 Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) membrane invagination other nucleus, endomembrane system 2889 BY4741 08_3 16 A 2 YHR198C AIM18 FMP22 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 2892 BY4741 08_3 16 A 3 YHR199C AIM46 FMP34 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 2893 BY4741 08_3 16 A 4 YHR200W RPN10 SUN1|MCB1 Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein proteolysis involved in cellular protein catabolic process small conjugating protein binding, structural molecule activity other 2894 BY4741 08_3 16 A 5 YHR202W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization biological_process molecular_function vacuole, cytoplasm 2896 BY4741 08_3 16 A 6 YHR203C RPS4B rp5|YS6|S7B|S4B Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to rat S4 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2897 BY4741 08_3 16 A 7 YHR204W MNL1 HTM1 Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation proteolysis involved in cellular protein catabolic process hydrolase activity, acting on glycosyl bonds endoplasmic reticulum, cytoplasm 2898 BY4741 08_3 16 A 8 YHR206W SKN7 POS9|BRY1 Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation transcription from RNA polymerase II promoter, response to oxidative stress, response to osmotic stress, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding, signal transducer activity nucleus 2900 BY4741 08_3 16 A 9 YHR207C SET5 - Putative S-adenosylmethionine-dependent methyltransferase; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus biological_process methyltransferase activity nucleus, cytoplasm 2901 BY4741 08_3 16 A 10 YHR209W CRG1 - S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin other RNA binding, methyltransferase activity, mRNA binding cellular_component 2903 BY4741 08_3 16 A 11 YHR210C - - Putative protein of unknown function; non-essential gene; highly expressed under anaeorbic conditions; sequence similarity to aldose 1-epimerases such as GAL10 biological_process molecular_function cellular_component 2904 BY4741 08_3 16 A 12 YCL001W RER1 - Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 3409 BY4741 03_1 16 B 1 YCL002C - - Putative protein of unknown function; YCL002C is not an essential gene biological_process molecular_function membrane 3410 BY4741 03_1 16 B 2 YCL005W LDB16 - Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria biological_process molecular_function mitochondrion, cytoplasm 3413 BY4741 03_1 16 B 3 YMR118C SHH3 - Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase biological_process molecular_function membrane 7121 BY4741 00_15 16 B 5 YCL008C STP22 VPL15|VPS23 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process small conjugating protein binding membrane, plasma membrane, cytoplasm 3416 BY4741 03_1 16 B 6 YCL009C ILV6 - Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria cellular amino acid metabolic process enzyme regulator activity mitochondrion, cytoplasm 3417 BY4741 03_1 16 B 7 YCL010C SGF29 - Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA recruitment to target promoters and H3 acetylation peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification protein binding transcription factor activity, histone binding nucleus 3418 BY4741 03_1 16 B 8 YCL011C GBP2 RLF6 Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro telomere organization, nucleobase-containing compound transport, nuclear transport RNA binding, DNA binding nucleus, cytoplasm 3419 BY4741 03_1 16 B 9 YCL012W - - - - - - 3420 BY4741 03_1 16 B 10 YCL013W - - - - - - 3421 BY4741 03_1 16 B 11 YCL014W BUD3 YCL012W Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding cell budding, cytokinesis molecular_function cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm 3422 BY4741 03_1 16 B 12 YCL016C DCC1 - Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance response to DNA damage stimulus, mitotic cell cycle, organelle fission, chromosome segregation molecular_function chromosome 3423 BY4741 03_1 16 C 1 YCL023C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 biological_process molecular_function cellular_component 3430 BY4741 03_1 16 C 2 YCL024W KCC4 - Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p protein phosphorylation, cell budding, cytoskeleton organization, cytokinesis, protein complex biogenesis, regulation of cell cycle kinase activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 3431 BY4741 03_1 16 C 3 YCL025C AGP1 YCC5 Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) transmembrane transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 3432 BY4741 03_1 16 C 4 YCL026C - - - - - - 3433 BY4741 03_1 16 C 5 YCL027W FUS1 - Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate cell morphogenesis, conjugation, response to chemical stimulus molecular_function membrane, site of polarized growth, plasma membrane 3434 BY4741 03_1 16 C 6 YCL028W RNQ1 [PIN(+)] [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate biological_process molecular_function cytoplasm 3435 BY4741 03_1 16 C 7 YCL029C BIK1 PAC14|ARM5 Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 mitotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, conjugation, regulation of organelle organization, protein complex biogenesis, nucleus organization cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, chromosome, microtubule organizing center, site of polarized growth, cytoplasm 3436 BY4741 03_1 16 C 8 YCL030C HIS4 - Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis cellular amino acid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, oxidoreductase activity other 3437 BY4741 03_1 16 C 9 YCL032W STE50 - Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction response to heat, conjugation, signaling, response to osmotic stress, response to chemical stimulus enzyme regulator activity cytoplasm 3439 BY4741 03_1 16 C 10 YCL033C MXR2 MSRB Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm 3440 BY4741 03_1 16 C 11 YCL034W LSB5 - Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat membrane invagination, cytoskeleton organization, endocytosis molecular_function cell cortex, cytoskeleton, cytoplasm 3441 BY4741 03_1 16 C 12 YCL036W GFD2 YCD6 Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation biological_process molecular_function cellular_component 3443 BY4741 03_1 16 D 1 YCL037C SRO9 - Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif cytoplasmic translation RNA binding other 3444 BY4741 03_1 16 D 2 YCL039W GID7 MOH2 Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm 3446 BY4741 03_1 16 D 3 YCL040W GLK1 HOR3 Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources carbohydrate metabolic process, carbohydrate transport, generation of precursor metabolites and energy kinase activity membrane fraction, cytoplasm 3447 BY4741 03_1 16 D 4 YCL042W - - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm 3449 BY4741 03_1 16 D 5 YCL044C MGR1 - Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm 3451 BY4741 03_1 16 D 6 YCL045C EMC1 - Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 3452 BY4741 03_1 16 D 7 YCL046W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C biological_process molecular_function cellular_component 3453 BY4741 03_1 16 D 8 YCL047C POF1 - Protein involved in the filamentation pathway; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion invasive growth in response to glucose limitation, pseudohyphal growth molecular_function cellular_component 3454 BY4741 03_1 16 D 9 YCL048W SPS22 - Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane 3455 BY4741 03_1 16 D 10 YCL049C - - Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane fraction 3456 BY4741 03_1 16 D 11 YCL050C APA1 DTP1 Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p nucleobase-containing small molecule metabolic process other nucleus, cytoplasm 3457 BY4741 03_1 16 D 12 YCL051W LRE1 - Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway cell wall organization or biogenesis molecular_function cellular_component 3458 BY4741 03_1 16 E 1 YCL055W KAR4 - Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone mitotic cell cycle, meiotic cell cycle, organelle fusion, response to chemical stimulus, conjugation, transcription from RNA polymerase II promoter, nucleus organization nucleic acid binding transcription factor activity nucleus, cytoplasm 3462 BY4741 03_1 16 E 2 YCL056C PEX34 - Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation peroxisome organization molecular_function peroxisome, membrane, cytoplasm 3463 BY4741 03_1 16 E 3 YCL057W PRD1 - Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins other peptidase activity mitochondrial envelope, mitochondrion, cytoplasm 3464 BY4741 03_1 16 E 4 YCL060C - - - - - - 3467 BY4741 03_1 16 E 5 YCL061C MRC1 YCL060C S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres mitotic cell cycle, DNA repair, telomere organization, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 3468 BY4741 03_1 16 E 6 YCL062W - - - - - - 3469 BY4741 03_1 16 E 7 YCL063W VAC17 YCL062W Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p vacuole organization, organelle inheritance other membrane, vacuole, cytoplasm 3470 BY4741 03_1 16 E 8 YCL064C CHA1 - Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 3471 BY4741 03_1 16 E 9 YCL069W VBA3 - Permease of basic amino acids in the vacuolar membrane transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm 3476 BY4741 03_1 16 E 10 YCR001W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene biological_process molecular_function cellular_component 3481 BY4741 03_1 16 E 11 YCR002C CDC10 - Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus contributes septin interaction with phosphatidylinositol-4,5-bisphosphate mitotic cell cycle, sporulation, cytoskeleton organization, cytokinesis, protein complex biogenesis GTPase activity, lipid binding, structural molecule activity cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm 3482 BY4741 03_1 16 E 12 YCR003W MRPL32 YmL32 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 3483 BY4741 03_1 16 F 1 YCR004C YCP4 - Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other membrane, membrane fraction, mitochondrion, cytoplasm 3484 BY4741 03_1 16 F 2 YCR005C CIT2 - Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors carbohydrate metabolic process, cellular amino acid metabolic process transferase activity, transferring acyl groups peroxisome, mitochondrion, cytoplasm 3485 BY4741 03_1 16 F 3 YCR006C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3486 BY4741 03_1 16 F 4 YCR007C - - Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene biological_process molecular_function cellular_component 3487 BY4741 03_1 16 F 5 YCR008W SAT4 HAL4 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p mitotic cell cycle, cellular ion homeostasis, protein dephosphorylation kinase activity cellular_component 3488 BY4741 03_1 16 F 6 YCR009C RVS161 SPE161|FUS7|END6 Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress cytoskeleton organization, membrane invagination, conjugation, response to starvation, response to osmotic stress, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, membrane, site of polarized growth, cytoplasm 3489 BY4741 03_1 16 F 7 YCR010C ADY2 ATO1 Acetate transporter required for normal sporulation; phosphorylated in mitochondria transmembrane transport, ion transport transmembrane transporter activity membrane fraction, mitochondrion, membrane, cytoplasm, plasma membrane 3490 BY4741 03_1 16 F 8 YCR011C ADP1 - Putative ATP-dependent permease of the ABC transporter family of proteins transmembrane transport ATPase activity, transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 3491 BY4741 03_1 16 F 9 YCR014C POL4 POLX DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta response to DNA damage stimulus, DNA repair nucleotidyltransferase activity nucleus 3494 BY4741 03_1 16 F 10 YCR015C - - Putative protein of unknown function; YCR015C is not an essential gene biological_process molecular_function cellular_component 3495 BY4741 03_1 16 F 11 YCR016W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus 3496 BY4741 03_1 16 F 12 YCR017C CWH43 - Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion lipid metabolic process, cell wall organization or biogenesis, protein lipidation molecular_function cellular bud, membrane, site of polarized growth, plasma membrane 3497 BY4741 03_1 16 G 1 YCR019W MAK32 - Protein necessary for structural stability of L-A double-stranded RNA-containing particles other molecular_function cellular_component 3499 BY4741 03_1 16 G 2 YCR020C PET18 HIT2 Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin vitamin metabolic process molecular_function cytoplasm 3500 BY4741 03_1 16 G 3 YCR020C-A MAK31 NAA38|SMX1|LSM9 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family protein acylation transferase activity, transferring acyl groups cytoplasm 3501 BY4741 03_1 16 G 4 YCR021C HSP30 YRO1 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase nucleobase-containing small molecule metabolic process molecular_function membrane, membrane fraction, plasma membrane 3502 BY4741 03_1 16 G 5 YCR022C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene biological_process molecular_function cellular_component 3503 BY4741 03_1 16 G 6 YCR023C - - Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 3504 BY4741 03_1 16 G 7 YLR420W URA4 - Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cytoplasm 4081 BY4741 12_3 16 G 8 YLR451W LEU3 - Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 4082 BY4741 12_3 16 G 9 YLR126C - - Putative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein cellular ion homeostasis molecular_function cytoplasm 4083 BY4741 12_3 16 G 10 YLR128W DCN1 - Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation regulation of protein modification process, protein modification by small protein conjugation or removal small conjugating protein binding, ligase activity cellular_component 4085 BY4741 12_3 16 G 11 YLR130C ZRT2 - Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane 4087 BY4741 12_3 16 G 12 YLR131C ACE2 - Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit of Ace2p during the M-to-G1 transition, causing its specific localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of the RAM network that regulates cellular polarity and morphogenesis mitotic cell cycle, cytokinesis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4088 BY4741 12_3 16 H 1 YLR133W CKI1 - Choline kinase, catalyzing the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway lipid metabolic process kinase activity cytoplasm 4090 BY4741 12_3 16 H 3 YLR134W PDC5 - Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism carbohydrate metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process lyase activity nucleus, cytoplasm 4091 BY4741 12_3 16 H 4 YLR135W SLX4 - Endonuclease involved in processing DNA during recombination and repair; cleaves branched structures in a complex with Slx1p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing response to DNA damage stimulus, DNA repair, DNA replication, DNA recombination nuclease activity nucleus, chromosome 4092 BY4741 12_3 16 H 5 YLR136C TIS11 CTH2 mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis RNA catabolic process, cellular ion homeostasis RNA binding, mRNA binding nucleus, cytoplasm 4093 BY4741 12_3 16 H 6 YLR137W RKM5 - Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species protein alkylation methyltransferase activity cellular_component 4094 BY4741 12_3 16 H 7 YLR138W NHA1 - Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH cellular ion homeostasis, response to osmotic stress transmembrane transporter activity membrane, plasma membrane 4095 BY4741 12_3 16 H 8 YLR139C SLS1 - Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery mitochondrial translation, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 4096 BY4741 12_3 16 H 9 YLR142W PUT1 - Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 4099 BY4741 12_3 16 H 10 YLR143W - - Putative protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; YLR143W is not an essential gene biological_process molecular_function cytoplasm 4100 BY4741 12_3 16 H 11 YLR144C ACF2 PCA1|ENG2 Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly cytoskeleton organization hydrolase activity, acting on glycosyl bonds other 4101 BY4741 12_3 16 H 12 YMR074C - - Protein with homology to human PDCD5, which is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene other molecular_function nucleus, cytoplasm 7118 BY4741 00_15 17 A 1 YLR149C - - Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene biological_process molecular_function cellular_component 4106 BY4741 12_3 17 A 2 YLR150W STM1 MPT4 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure translational initiation, telomere organization, regulation of translation, translational elongation, signaling DNA binding ribosome, cytoplasm 4107 BY4741 12_3 17 A 3 YLR151C PCD1 - Peroxisomal nudix pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids biological_process other peroxisome, cytoplasm 4108 BY4741 12_3 17 A 4 YLR152C - - Putative protein of unknown function; YLR152C is not an essential gene biological_process molecular_function membrane 4109 BY4741 12_3 17 A 5 YLR154C RNH203 Rnh2C Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome RNA catabolic process, DNA replication nuclease activity nucleus, cytoplasm 4111 BY4741 12_3 17 A 6 YLR164W SHH4 - Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4113 BY4741 12_3 17 A 7 YLR165C PUS5 - Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability rRNA processing, RNA modification lyase activity mitochondrion, cytoplasm 4114 BY4741 12_3 17 A 8 YLR168C UPS2 GEP1|AIM30|MSF1|MSF1' Mitochondrial intermembrane space protein involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI lipid metabolic process, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4117 BY4741 12_3 17 A 9 YLR169W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4118 BY4741 12_3 17 A 10 YLR170C APS1 YAP19 Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 4119 BY4741 12_3 17 A 11 YLR171W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 4120 BY4741 12_3 17 A 12 YLR172C DPH5 - Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm peptidyl-amino acid modification methyltransferase activity cytoplasm 4121 BY4741 12_3 17 B 1 YLR173W - - Putative protein of unknown function biological_process molecular_function cellular_component 4122 BY4741 12_3 17 B 2 YLR174W IDP2 - Cytosolic NADP-specific isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose cellular amino acid metabolic process oxidoreductase activity cytoplasm 4123 BY4741 12_3 17 B 3 YLR176C RFX1 CRT1 Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins transcription from RNA polymerase II promoter DNA binding nucleus, cytoplasm 4125 BY4741 12_3 17 B 4 YLR177W - - Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; YLR177W is not an essential gene biological_process molecular_function cytoplasm 4126 BY4741 12_3 17 B 6 YLR178C TFS1 DKA1 Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase signaling lipid binding, enzyme regulator activity membrane, vacuole, cytoplasm 4127 BY4741 12_3 17 B 7 YLR179C - - Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm 4128 BY4741 12_3 17 B 8 YLR180W SAM1 ETH10 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 4129 BY4741 12_3 17 B 9 YLR181C VTA1 - Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain endosome transport, protein complex biogenesis enzyme regulator activity membrane, membrane fraction, cytoplasm 4130 BY4741 12_3 17 B 10 YLR182W SWI6 SDS11|PSL8 Transcription cofactor, forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization mitotic cell cycle, meiotic cell cycle, response to chemical stimulus, mRNA processing, transcription from RNA polymerase II promoter, signaling protein binding transcription factor activity nucleus, cytoplasm 4131 BY4741 12_3 17 B 11 YLR183C TOS4 - Forkhead Associated domain containing protein and putative transcription factor found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; similar to PLM2 response to DNA damage stimulus other nucleus, chromosome, mitochondrion, cytoplasm 4132 BY4741 12_3 17 B 12 YLR184W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4133 BY4741 12_3 17 C 1 YLR185W RPL37A YL35|L43|L37A Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Bp and to rat L37 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4134 BY4741 12_3 17 C 2 YLR187W SKG3 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p biological_process molecular_function cellular bud, site of polarized growth, cytoplasm 4136 BY4741 12_3 17 C 3 YLR188W MDL1 - Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress transmembrane transport ATPase activity, transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4137 BY4741 12_3 17 C 4 YLR189C ATG26 UGT51 UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy lipid metabolic process, sporulation transferase activity, transferring glycosyl groups cytoplasm 4138 BY4741 12_3 17 C 5 YLR190W MMR1 - Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p mitochondrion organization, organelle inheritance molecular_function mitochondrion, cellular bud, membrane, site of polarized growth, mitochondrial envelope, cytoplasm 4139 BY4741 12_3 17 C 6 YLR191W PEX13 PAS20 Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p protein targeting, peroxisome organization, protein complex biogenesis protein binding, bridging peroxisome, membrane, cytoplasm 4140 BY4741 12_3 17 C 7 YLR193C UPS1 - Mitochondrial intermembrane space protein that regulates mitochondrial cardiolipin levels, null has defects in Mgm1p processing, integrity of mitochondrial inner membrane complexes, and mitochondrial morphology; ortholog of human PRELI lipid metabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4142 BY4741 12_3 17 C 8 YLR194C - - Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress cell wall organization or biogenesis structural molecule activity cell wall, membrane, plasma membrane 4143 BY4741 12_3 17 C 9 YLR199C PBA1 POC1 Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly protein complex biogenesis molecular_function cytoplasm 4148 BY4741 12_3 17 C 10 YLR200W YKE2 PFD6|GIM1 Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin protein folding, protein complex biogenesis cytoskeletal protein binding cytoplasm 4149 BY4741 12_3 17 C 11 YLR201C COQ9 FMP53 Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 4150 BY4741 12_3 17 C 12 YLR202C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron biological_process molecular_function cellular_component 4151 BY4741 12_3 17 D 1 YLR203C MSS51 - Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 4152 BY4741 12_3 17 D 2 YLR204W QRI5 COX24 Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA mRNA processing molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4153 BY4741 12_3 17 D 3 YLR205C HMX1 - ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants cofactor metabolic process, response to oxidative stress, response to chemical stimulus, cellular ion homeostasis oxidoreductase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4154 BY4741 12_3 17 D 4 YLR206W ENT2 - Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus cytoskeleton organization, membrane invagination, endocytosis other cell cortex, cytoskeleton, site of polarized growth, cytoplasm 4155 BY4741 12_3 17 D 5 YLR207W HRD3 - Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events proteolysis involved in cellular protein catabolic process ligase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4156 BY4741 12_3 17 D 6 YLR209C PNP1 - Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, hydrolase activity, acting on glycosyl bonds other 4158 BY4741 12_3 17 D 7 YLR210W CLB4 - B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation mitotic cell cycle, protein phosphorylation, cytoskeleton organization, regulation of organelle organization, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cytoskeleton, microtubule organizing center, cytoplasm 4159 BY4741 12_3 17 D 8 YLR211C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron biological_process molecular_function cytoplasm 4160 BY4741 12_3 17 D 9 YLR213C CRR1 - Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation sporulation, cell wall organization or biogenesis hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cell wall 4162 BY4741 12_3 17 D 10 YLR214W FRE1 - Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels transmembrane transport, ion transport oxidoreductase activity membrane, membrane fraction, plasma membrane 4163 BY4741 12_3 17 D 11 YLR216C CPR6 CYP40 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity protein folding unfolded protein binding, isomerase activity cytoplasm 4165 BY4741 12_3 17 D 12 YLR217W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 biological_process molecular_function cellular_component 4166 BY4741 12_3 17 E 1 YLR218C COA4 CMC3 Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors mitochondrion organization, protein complex biogenesis molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 4167 BY4741 12_3 17 E 2 YLR219W MSC3 - Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate meiotic cell cycle, DNA recombination molecular_function cellular_component 4168 BY4741 12_3 17 E 3 YLR220W CCC1 - Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation cellular ion homeostasis, ion transport transmembrane transporter activity membrane, membrane fraction, vacuole, cytoplasm, Golgi apparatus 4169 BY4741 12_3 17 E 4 YLR221C RSA3 - Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 4170 BY4741 12_3 17 E 5 YLR224W - - F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene proteolysis involved in cellular protein catabolic process, response to chemical stimulus protein binding, bridging, ligase activity other 4173 BY4741 12_3 17 E 6 YLR225C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene biological_process molecular_function cytoplasm 4174 BY4741 12_3 17 E 7 YLR227C ADY4 - Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane sporulation structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 4176 BY4741 12_3 17 E 8 YKL001C MET14 - Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism cellular amino acid metabolic process kinase activity cytoplasm 4849 BY4741 11_1 17 E 9 YKL002W DID4 VPS14|CHM2|VPS2|VPT14|VPL2|REN1|GRD7 Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis endosome transport, vesicle organization, proteolysis involved in cellular protein catabolic process molecular_function membrane, cytoplasm 4850 BY4741 11_1 17 E 10 YKL003C MRP17 - Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4851 BY4741 11_1 17 E 11 YKL006W RPL14A L14A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to rat L14 ribosomal protein; rpl14a csh5 double null mutant exhibits synthetic slow growth cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4855 BY4741 11_1 17 E 12 YKL007W CAP1 - Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches cytoskeleton organization, regulation of organelle organization, protein complex biogenesis cytoskeletal protein binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm 4856 BY4741 11_1 17 F 1 YKL008C LAC1 DGT1 Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p lipid metabolic process transferase activity, transferring acyl groups membrane, endoplasmic reticulum, cytoplasm 4857 BY4741 11_1 17 F 2 YKL009W MRT4 - Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus rRNA processing, organelle assembly, ribosomal large subunit biogenesis, RNA catabolic process, ribosome assembly RNA binding, rRNA binding nucleus, nucleolus 4858 BY4741 11_1 17 F 3 YKL010C UFD4 - Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins protein modification by small protein conjugation or removal ligase activity mitochondrion, cytoplasm 4859 BY4741 11_1 17 F 4 YKL011C CCE1 MGT1 Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA mitochondrion organization nuclease activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4860 BY4741 11_1 17 F 5 YKL015W PUT3 - Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitogen sources; has a Zn(2)-Cys(6) binuclear cluster domain transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 4864 BY4741 11_1 17 F 6 YKL016C ATP7 - Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4865 BY4741 11_1 17 F 7 YKL017C HCS1 DIP1 Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities DNA replication helicase activity, ATPase activity nucleus, chromosome 4866 BY4741 11_1 17 F 8 YKL020C SPT23 - ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting lipid metabolic process, transcription from RNA polymerase II promoter DNA binding nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4869 BY4741 11_1 17 F 9 YKL023W - - Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 4872 BY4741 11_1 17 F 10 YKL025C PAN3 ECM35 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes response to DNA damage stimulus, DNA repair, mRNA processing nuclease activity cytoplasm 4874 BY4741 11_1 17 F 11 YKL026C GPX1 - Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity cellular_component 4875 BY4741 11_1 17 F 12 YKL027W - - Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4876 BY4741 11_1 17 G 1 YKL029C MAE1 - Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 4878 BY4741 11_1 17 G 2 YKL031W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species biological_process molecular_function cellular_component 4880 BY4741 11_1 17 G 3 YKL032C IXR1 ORD1 Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b response to DNA damage stimulus, DNA repair, transcription from RNA polymerase II promoter, response to chemical stimulus DNA binding nucleus, chromosome 4881 BY4741 11_1 17 G 4 YKL034W TUL1 - Golgi-localized RING-finger ubiquitin ligase (E3), involved in ubiquitinating and sorting membrane proteins that contain polar transmembrane domains to multivesicular bodies for delivery to the vacuole for quality control purposes protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity membrane, cytoplasm, Golgi apparatus 4883 BY4741 11_1 17 G 5 YKL037W AIM26 - Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT other molecular_function membrane, mitochondrion, cytoplasm 4886 BY4741 11_1 17 G 6 YKL038W RGT1 - Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor carbohydrate metabolic process, carbohydrate transport,