heterozygous essentials b isolates release 1 Synced to SGD on Wed Feb 1 10:00:12 2012 Plate # Row Column ORF Gene Alias Description GO Biological Process GO Molecular Function GO Cellular Component record no. strain rg notes db notes 401 A 2 YAL043C PTA1 FUN39 Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII tRNA processing, transcription termination, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter RNA binding nucleus 20362 chr1_1 - - 401 A 3 YAL041W CDC24 CLS4 Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing mitotic cell cycle, regulation of cell cycle, signaling enzyme regulator activity nucleus, cellular bud, site of polarized growth, cytoplasm 20365 chr1_1 met- did not mate 401 A 4 YAL038W CDC19 PYK1 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration carbohydrate metabolic process, generation of precursor metabolites and energy kinase activity membrane fraction, cytoplasm 20368 chr1_1 - - 401 A 5 YAL034W-a - - - - - - 20372 chr1_1 - - 401 A 6 YAL035C-a - - - - - - 20373 chr1_1 - - 401 A 7 YAL033W POP5 FUN53 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs snoRNA processing, tRNA processing, rRNA processing, mRNA processing nuclease activity nucleus, nucleolus 20375 chr1_1 - - 401 A 8 YAL032C PRP45 FUN20 Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene RNA splicing, mRNA processing other nucleus 20376 chr1_1 - - 401 A 9 YAL025C MAK16 - Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus rRNA processing, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 20383 chr1_1 - - 401 A 10 YAL003W EFB1 EF-1beta|eEF1Balpha|TEF5 Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site translational elongation RNA binding, translation factor activity, nucleic acid binding, enzyme regulator activity ribosome, cytoplasm 20404 chr1_1 - - 401 A 11 YAL001C TFC3 tau 138|TSV115|FUN24 Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding transcription from RNA polymerase III promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome, mitochondrion, cytoplasm 20406 chr1_1 - - 401 A 12 YAR007C RFA1 RPA1|FUN3|BUF2 Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination meiotic cell cycle, DNA repair, telomere organization, DNA replication, response to DNA damage stimulus, protein modification by small protein conjugation or removal, DNA recombination DNA binding nucleus, chromosome, cytoplasm 20409 chr1_1 - - 401 B 1 YAR008W SEN34 tRNA splicing endonuclease subunit|FUN4 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease RNA splicing, tRNA processing nuclease activity nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm 20410 chr1_1 - - 401 B 2 YAR019C CDC15 LYT1 Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II mitotic cell cycle, meiotic cell cycle, protein phosphorylation, cytokinesis, regulation of cell cycle kinase activity cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm 20416 chr1_1 - - 401 B 3 YLL003W SFI1 - Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C mitotic cell cycle, cytoskeleton organization, organelle assembly molecular_function cytoskeleton, microtubule organizing center, cytoplasm 21491 chr12_1 - - 401 B 4 YLL004W ORC3 - Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing DNA replication DNA binding nucleus, chromosome 21492 chr12_1 - - 401 B 5 YLL008W DRS1 - Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis helicase activity, ATPase activity nucleus, nucleolus 21496 chr12_1 - - 401 B 6 YLL011W SOF1 - Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 21499 chr12_1 - - 401 B 7 YLL018C DPS1 AspRS|aspartyl-tRNA synthetase Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation cytoplasmic translation, tRNA aminoacylation for protein translation, cellular amino acid metabolic process RNA binding, ligase activity nucleus, cytoplasm 21506 chr12_1 - - 401 B 8 YLL031C GPI13 MPC1 ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein lipid metabolic process, protein lipidation other membrane, endoplasmic reticulum, cytoplasm 21519 chr12_1 - - 401 B 9 YLL034C RIX7 - Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport ATPase activity nucleus, nucleolus 21522 chr12_1 - - 401 B 10 YLL035W GRC3 - Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated transcription from RNA polymerase I promoter, rRNA processing, transcription termination, DNA-dependent kinase activity nucleus, nucleolus, chromosome 21523 chr12_1 - - 401 B 11 YLL036C PRP19 PSO4 Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain RNA splicing, mRNA processing ligase activity nucleus, mitochondrion, cytoplasm 21524 chr12_1 - - 401 B 12 YLL037W - - Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor biological_process molecular_function cellular_component 21525 chr12_1 - - 401 C 1 YLL050C COF1 cofilin Cofilin, promotes actin filament depolarization in a pH-dependent manner; binds both actin monomers and filaments and severs filaments; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes cytoskeleton organization cytoskeletal protein binding cell cortex, membrane fraction, cytoskeleton, cytoplasm 21538 chr12_1 - - 401 C 2 YLR002C NOC3 - Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation DNA replication, rRNA processing other nucleus, nucleolus, cytoplasm 21557 chr12_1 - - 401 C 3 YLR005W SSL1 - Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p response to DNA damage stimulus, DNA repair, translational initiation, protein phosphorylation, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 21560 chr12_1 - - 401 C 4 YLR007W NSE1 - Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair response to DNA damage stimulus, DNA repair molecular_function nucleus, chromosome 21562 chr12_1 - - 401 C 5 YLR008C PAM18 TIM14 Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p protein targeting, mitochondrion organization, transmembrane transport enzyme regulator activity, unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 21563 chr12_1 - - 401 C 6 YLR009W RLP24 - Essential protein with similarity to Rpl24Ap and Rpl24Bp, associated with pre-60S ribosomal subunits and required for ribosomal large subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 21564 chr12_1 - - 401 C 7 YLR010C TEN1 - Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p telomere organization, DNA replication, regulation of organelle organization, regulation of DNA metabolic process DNA binding nucleus, chromosome 21565 chr12_1 - - 401 C 8 YLR022C SDO1 - Essential protein involved in 60S ribosome maturation; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archae and eukaryotes rRNA processing, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport molecular_function nucleus, cytoplasm 21577 chr12_1 - - 401 C 9 YLR026C SED5 - cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins membrane fusion, Golgi vesicle transport, organelle fusion, vesicle organization other membrane, cytoplasm, Golgi apparatus 21581 chr12_1 - - 401 C 10 YLR029C RPL15A rp15R|YL10|L15A|L13A|RPL10A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 21584 chr12_1 - - 401 C 11 YLR045C STU2 - Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p mitotic cell cycle, cytoskeleton organization cytoskeletal protein binding, structural molecule activity nucleus, cell cortex, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 22656 chr12_2 - - 401 C 12 YLR051C FCF2 - Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 22662 chr12_2 - - 401 D 1 YLR060W FRS1 cytoplasmic phenylalanyl-tRNA synthetase beta subunit Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity cytoplasm 22671 chr12_2 - - 401 D 2 YLR066W SPC3 - Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 protein targeting, protein maturation peptidase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 22677 chr12_2 - - 401 D 3 YLR071C RGR1 MED14 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation transcription from RNA polymerase II promoter transcription factor binding nucleus 22682 chr12_2 - - 401 D 4 YLR075W RPL10 L10|QSR1|GRC5 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 22686 chr12_2 - - 401 D 5 YLR076C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 biological_process molecular_function cellular_component 22687 chr12_2 - - 401 D 6 YLR078C BOS1 SEC32 v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion other endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, cytoplasm 22689 chr12_2 - - 401 D 7 YLR086W SMC4 - Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate mitotic cell cycle, organelle fission, chromosome segregation ATPase activity nucleus, chromosome 22697 chr12_2 - - 401 D 8 YLR088W GAA1 END2 Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER lipid metabolic process, protein lipidation other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 22699 chr12_2 - - 401 D 9 YLR100W ERG27 - 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs lipid metabolic process oxidoreductase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 22711 chr12_2 - - 401 D 10 YLR101C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W biological_process molecular_function membrane 22712 chr12_2 - - 401 D 11 YLR103C CDC45 SLD4 DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination DNA binding nucleus, chromosome 22714 chr12_2 - - 401 D 12 YLR105C SEN2 tRNA splicing endonuclease subunit Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease RNA splicing, tRNA processing nuclease activity nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm 22716 chr12_2 - - 401 E 1 YLR106C MDN1 REA1 Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis ATPase activity nucleus, mitochondrion, cytoplasm 22717 chr12_2 - - 401 E 2 YLR115W CFT2 YDH1 Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. mRNA processing RNA binding nucleus 22726 chr12_2 - - 401 E 3 YLR116W MSL5 BBP Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA RNA splicing, mRNA processing RNA binding nucleus 22727 chr12_2 - - 401 E 4 YLR117C CLF1 NTC77|SYF3 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins RNA splicing, DNA replication, mRNA processing DNA binding nucleus, chromosome 22728 chr12_2 - - 401 E 5 YML043C RRN11 - Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription from RNA polymerase I promoter, transcription initiation, DNA-dependent protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus, nucleolus 20528 chr13_1b - - 401 E 6 YML049C RSE1 - Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport RNA splicing, mRNA processing RNA binding nucleus 20522 chr13_1b - - 401 E 7 YML064C TEM1 - GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis mitotic cell cycle, regulation of cell cycle GTPase activity cytoskeleton, microtubule organizing center, cytoplasm 20506 chr13_1b - - 401 E 8 YML065W ORC1 - Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity DNA replication DNA binding, ATPase activity nucleus, chromosome 20505 chr13_1b - - 401 E 9 YML010W SPT5 - Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a complex with Spt4p; contains a C-terminal repeat domain that is a target for phosphorylation by Sgv1p transcription from RNA polymerase I promoter, DNA repair, chromatin organization, response to DNA damage stimulus, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, enzyme binding nucleus, chromosome, mitochondrion, cytoplasm 20564 chr13_2 - - 401 E 10 YML025C YML6 YmL6 Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 20548 chr13_2 - - 401 E 11 YMR001C CDC5 PKX2|MSD2 Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate meiotic cell cycle, protein phosphorylation, cytoskeleton organization, regulation of organelle organization, organelle assembly, chromosome segregation, DNA recombination, regulation of cell cycle kinase activity nucleus, cytoskeleton, cellular bud, site of polarized growth 20576 chr13_2 - - 401 E 12 YMR013C SEC59 - Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation protein glycosylation, carbohydrate metabolic process kinase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 20588 chr13_2 - - 401 F 1 YMR028W TAP42 - Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits transcription from RNA polymerase I promoter, signaling molecular_function membrane fraction 20603 chr13_2 - - 401 F 2 YMR043W MCM1 FUN80 Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes DNA replication, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 20619 chr13_2 - - 401 F 3 YMR146C TIF34 - eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation cytoplasmic translation, translational initiation RNA binding, translation factor activity, nucleic acid binding cytoplasm 20727 chr13_4 - - 401 F 4 YMR149W SWP1 - Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 20730 chr13_4 - - 401 F 5 YMR168C CEP3 CSL1|CBF3B|CBF3 Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis, regulation of cell cycle DNA binding nucleus, chromosome 20751 chr13_4 - - 401 F 6 YMR197C VTI1 - Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways vacuole organization, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion other endomembrane system, membrane, Golgi apparatus, cytoplasm 20783 chr13_4 - - 401 F 7 YMR200W ROT1 - Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations cell budding, cytoskeleton organization, cytokinesis, protein folding, cell wall organization or biogenesis unfolded protein binding endomembrane system, membrane, endoplasmic reticulum, cytoplasm 20786 chr13_4 - - 401 F 8 YMR203W TOM40 MOM38|ISP42 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 20789 chr13_4 - - 401 F 9 YMR208W ERG12 RAR1 Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate lipid metabolic process, cofactor metabolic process kinase activity cytoplasm 20794 chr13_4 - - 401 F 10 YJL061W NUP82 HRB187 Nucleoporin, subunit of the nuclear pore complex (NPC); forms a subcomplex with Gle2p, Nup159p, Nsp1p, and Nup116p and is required for proper localization of Nup116p in the NPC protein targeting, ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport molecular_function nucleus, endomembrane system, membrane 21362 chr10_2 - - 401 F 11 YMR213W CEF1 NTC85 Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p RNA splicing, mRNA processing other nucleus 20799 chr13_4 - - 401 F 12 YMR218C TRS130 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1 Golgi vesicle transport, endosome transport, nucleobase-containing small molecule metabolic process, signaling enzyme regulator activity cytoplasm, Golgi apparatus 20804 chr13_4 - - 401 G 1 YMR220W ERG8 - Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate lipid metabolic process, cofactor metabolic process kinase activity nucleus, cytoplasm 20806 chr13_4 - - 401 G 2 YMR229C RRP5 - RNA binding protein with preference for single stranded tracts of U's involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome rRNA processing, ribosomal large subunit biogenesis, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 20815 chr13_4 - - 401 G 3 YMR235C RNA1 - GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport protein targeting, ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport enzyme regulator activity nucleus, membrane, cytoplasm 20821 chr13_5 - - 401 G 4 YMR236W TAF9 TafII17|TAF17 Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 peptidyl-amino acid modification, protein acylation, chromatin organization, transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, histone modification nucleic acid binding transcription factor activity, structural molecule activity nucleus 20822 chr13_5 - - 401 G 5 YMR239C RNT1 - RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination chromatin organization, rRNA processing, mRNA processing, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter nuclease activity nucleus, nucleolus 20825 chr13_5 - - 401 G 6 YMR240C CUS1 - Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p RNA splicing, mRNA processing molecular_function nucleus 20826 chr13_5 - - 401 G 7 YMR260C TIF11 - Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 translational initiation RNA binding, translation factor activity, nucleic acid binding ribosome, cytoplasm 20846 chr13_5 - - 401 G 8 YMR268C PRP24 - Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling RNA splicing, mRNA processing RNA binding nucleus 20854 chr13_5 - - 401 G 9 YMR270C RRN9 - Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I transcription from RNA polymerase I promoter nucleic acid binding transcription factor activity, transcription factor binding nucleus, nucleolus 20856 chr13_5 - - 401 G 10 YMR281W GPI12 - ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp lipid metabolic process, protein lipidation hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds membrane, endomembrane system, endoplasmic reticulum, cytoplasm 20867 chr13_5 - - 401 G 11 YMR288W HSH155 - U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 20874 chr13_5 - - 401 G 12 YMR290C HAS1 - ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles rRNA processing RNA binding, helicase activity, ATPase activity nucleus, nucleolus, endomembrane system 20877 chr13_5 - - 401 H 1 YMR290W-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase biological_process molecular_function cellular_component 20876 chr13_5 - - 401 H 2 YMR296C LCB1 TSC2|END8 Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine lipid metabolic process transferase activity, transferring acyl groups membrane, membrane fraction, endomembrane system, endoplasmic reticulum, cytoplasm 20884 chr13_5 met- did not mate 401 H 3 YMR298W LIP1 - Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis lipid metabolic process transferase activity, transferring acyl groups nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 20886 chr13_5 - - 401 H 4 YMR301C ATM1 - Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 20889 chr13_5 - - 401 H 6 YMR308C PSE1 KAP121 Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p mitotic cell cycle, protein targeting, organelle fission, regulation of organelle organization, nucleobase-containing compound transport, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport, regulation of cell cycle protein transporter activity nucleus, membrane, cytoplasm 20898 chr13_5 - - 401 H 7 YMR309C NIP1 - eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection translational initiation RNA binding, translation factor activity, nucleic acid binding cytoplasm 20899 chr13_5 - - 401 H 8 YOR004W UTP23 - Essential nucleolar protein that is a component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus, mitochondrion, cytoplasm 21780 chr15_1 - - 401 H 9 YOR020C HSP10 CPN10 Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES protein folding unfolded protein binding mitochondrion, cytoplasm 21796 chr15_1 met- did not mate 401 H 10 YOR046C DBP5 RAT8 Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p nucleobase-containing compound transport, nuclear transport ATPase activity, helicase activity nucleus, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm 21822 chr15_1 - - 401 H 11 YOR048C RAT1 XRN2|TAP1|HKE1 Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination RNA catabolic process, rRNA processing, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter nuclease activity nucleus, mitochondrion, cytoplasm 21824 chr15_1 - - 401 H 12 YOR056C NOB1 YOR29-07 Essential nuclear protein involved in proteasome maturation and synthesis of 40S ribosomal subunits; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA rRNA processing, ribosomal small subunit biogenesis, protein complex biogenesis RNA binding, rRNA binding, nuclease activity nucleus, cytoplasm 21832 chr15_1 - - 402 A 1 YOR057W SGT1 YOR29-08 Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes protein modification by small protein conjugation or removal, organelle assembly, signaling, regulation of cell cycle, protein complex biogenesis protein binding, bridging other 21833 chr15_1 - - 402 A 3 YOR060C SLD7 YOR29-11 Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria DNA replication, regulation of DNA metabolic process molecular_function nucleus, cytoskeleton, microtubule organizing center, endomembrane system, endoplasmic reticulum, cytoplasm 21836 chr15_1 - - 402 A 4 YOR063W RPL3 rp1|YL1|L3|YOR29-14|TCM1|MAK8 Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribosomal proteins; involved in the replication and maintenance of killer double stranded RNA virus cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 21839 chr15_1 - - 402 A 5 YOR074C CDC21 YOR29-25|TMP1|CRT9 Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S nucleobase-containing small molecule metabolic process methyltransferase activity nucleus 21850 chr15_1 - - 402 A 6 YOR075W UFE1 YOR29-26 t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion other membrane, endoplasmic reticulum, cytoplasm 21851 chr15_1 - - 402 A 7 YOR077W RTS2 YOR29-28 Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication biological_process molecular_function nucleus, cytoplasm 21853 chr15_1 - - 402 A 8 YOR095C RKI1 - Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, vitamin metabolic process isomerase activity nucleus, cytoplasm 21871 chr15_1 - - 402 A 9 YOR294W RRS1 - Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice rRNA processing, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, ribosomal small subunit biogenesis, nuclear transport molecular_function nucleus, nucleolus 21590 chr15_4 - - 402 A 10 YOR310C NOP58 NOP5 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 21607 chr15_4 met- did not mate 402 A 11 YJL072C PSF2 CDC102 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery response to DNA damage stimulus, DNA repair, DNA replication, DNA recombination molecular_function nucleus, chromosome 21351 chr10_2 - - 402 A 12 YOR326W MYO2 CDC66 One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle vacuole organization, mitotic cell cycle, cytoskeleton organization, peroxisome organization, cell budding, cell morphogenesis, mitochondrion organization, cytokinesis, organelle inheritance cytoskeletal protein binding cytoskeleton, vacuole, cellular bud, membrane, site of polarized growth, cytoplasm 21623 chr15_4 - - 402 B 1 YOR329C SCD5 FTB1 Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency cytoskeleton organization, membrane invagination, endocytosis enzyme binding nucleus, cell cortex, membrane fraction, cytoskeleton, cytoplasm 21626 chr15_4 - - 402 B 2 YOR335C ALA1 CDC64 Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 21632 chr15_4 - - 402 B 3 YJL069C UTP18 - Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 21354 chr10_2 - - 402 B 4 YOR341W RPA190 A190|RRN1 RNA polymerase I largest subunit A190 transcription from RNA polymerase I promoter nucleotidyltransferase activity nucleus, nucleolus 21638 chr15_4 - - 402 B 5 YOR353C SOG2 - Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis cell budding, cytokinesis, signaling molecular_function cellular bud, site of polarized growth, cytoplasm 21650 chr15_4 - - 402 B 6 YOR361C PRT1 DNA26|CDC63 eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes translational initiation RNA binding, translation factor activity, nucleic acid binding cytoplasm 21658 chr15_4 - - 402 B 7 YOR362C PRE10 - Alpha 7 subunit of the 20S proteasome proteolysis involved in cellular protein catabolic process RNA binding, mRNA binding nucleus, endomembrane system, mitochondrion, membrane, cytoplasm 21659 chr15_4 - - 402 B 8 YOR370C MRS6 MSI4 Rab escort protein, forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene protein targeting, Golgi vesicle transport, nucleobase-containing small molecule metabolic process, signaling enzyme binding, transferase activity, transferring alkyl or aryl (other than methyl) groups membrane, cytoplasm 21667 chr15_4 - - 402 B 9 YOR372C NDD1 - Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes mitotic cell cycle, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 21669 chr15_4 - - 402 B 10 YOR373W NUD1 - Component of the spindle pole body outer plaque, required for exit from mitosis mitotic cell cycle, meiotic cell cycle, chromosome segregation structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 21670 chr15_4 - - 402 B 11 YOR336W KRE5 - Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects carbohydrate metabolic process transferase activity, transferring glycosyl groups endoplasmic reticulum, cytoplasm 21633 chr15_4 - - 402 B 12 YOL005C RPB11 B12.5 RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit transcription termination, DNA-dependent, transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus 21696 chr15_5 - - 402 C 1 YOL010W RCL1 - Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected rRNA processing, ribosomal small subunit biogenesis nuclease activity, enzyme regulator activity nucleus, nucleolus 21701 chr15_5 - - 402 C 2 YOL021C DIS3 MTR17|RRP44 Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3 snoRNA processing, rRNA processing, RNA catabolic process RNA binding, nuclease activity nucleus, mitochondrion, nucleolus, cytoplasm 21712 chr15_5 - - 402 C 3 YOL022C TSR4 - Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 rRNA processing, ribosomal small subunit biogenesis molecular_function cytoplasm 21713 chr15_5 - - 402 C 4 YOL026C MIM1 TOM13 Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present as an oligomer in the outer membrane that may create a local environment that facilitates membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex protein targeting, mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 21717 chr15_5 - - 402 C 5 YOL034W SMC5 - Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair DNA repair, chromosome segregation, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity nucleus, chromosome 21725 chr15_5 - - 402 C 6 YOL038W PRE6 - Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress proteolysis involved in cellular protein catabolic process molecular_function nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 21729 chr15_5 - - 402 C 7 YOL040C RPS15 rp52|S21|S15|RIG protein|RPS21 Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ribosomal proteins cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 21731 chr15_5 - - 402 C 8 YOL066C RIB2 PUS8 Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs tRNA processing, vitamin metabolic process, RNA modification hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, isomerase activity cytoplasm 21758 chr15_5 - - 402 C 9 YOL069W NUF2 - Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering cytoskeleton organization, chromosome segregation structural molecule activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 21761 chr15_5 - - 402 C 10 YOL077C BRX1 - Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif ribosome assembly, organelle assembly, ribosomal large subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus 21768 chr15_5 - - 402 C 11 YPL190C NAB3 HMD1 Single stranded RNA binding protein; subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); acidic ribonucleoprotein; required for termination of non-poly(A) transcripts and efficient splicing transcription termination, DNA-dependent, transcription from RNA polymerase II promoter RNA binding, mRNA binding nucleus 21102 chr16_1 - - 402 C 12 YPL204W HRR25 KTI14 Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, response to DNA damage stimulus, Golgi vesicle transport, tRNA processing, RNA modification, DNA repair, protein phosphorylation, ribosomal small subunit biogenesis, chromosome segregation kinase activity nucleus, cytoskeleton, chromosome, plasma membrane, microtubule organizing center, cellular bud, membrane, site of polarized growth, cytoplasm 21088 chr16_1 - - 402 D 1 YPL209C IPL1 PAC15 Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation mitotic cell cycle, meiotic cell cycle, protein phosphorylation, cytoskeleton organization, cytokinesis, chromosome segregation, regulation of cell cycle kinase activity nucleus, cytoskeleton, chromosome 21083 chr16_1 - - 402 D 2 YPL210C SRP72 - Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane protein targeting, protein complex biogenesis other cytoplasm 21082 chr16_1 - - 402 D 3 YPL211W NIP7 - Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p rRNA processing, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly molecular_function nucleus, ribosome, nucleolus, cytoplasm 21081 chr16_1 - - 402 D 4 YPL218W SAR1 - GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport regulation of organelle organization, regulation of transport, vesicle organization, Golgi vesicle transport, nucleobase-containing small molecule metabolic process GTPase activity endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm 21074 chr16_1 - - 402 D 5 YPL228W CET1 CES5 Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide mRNA processing phosphatase activity nucleus 21064 chr16_1 - - 402 D 6 YPL231W FAS2 - Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities lipid metabolic process transferase activity, transferring acyl groups, oxidoreductase activity mitochondrion, cytoplasm 21061 chr16_1 - - 402 D 7 YPL233W NSL1 - Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nucleus, cytoskeleton, chromosome 21059 chr16_1 - - 402 D 8 YPL235W RVB2 TIP48|TIH2|TIP49B Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; also referred to as reptin; member of the RUVB-like protein family chromatin organization, rRNA processing, transcription from RNA polymerase II promoter helicase activity, ATPase activity nucleus, chromosome 21057 chr16_1 - - 402 D 9 YPL237W SUI3 - Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding translational initiation RNA binding, translation factor activity, nucleic acid binding ribosome, cytoplasm 21054 chr16_1 - - 402 D 10 YPL238C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W biological_process molecular_function membrane 21055 chr16_1 - - 402 D 11 YPL242C IQG1 CYK1 Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family cytoskeleton organization, cytokinesis, response to osmotic stress cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 21050 chr16_1 - - 402 D 12 YPL243W SRP68 - Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane protein targeting, protein complex biogenesis other cytoplasm 21049 chr16_1 - - 402 E 1 YPL251W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C biological_process molecular_function cellular_component 21040 chr16_1 - - 402 E 2 YPL252C YAH1 - Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin cofactor metabolic process oxidoreductase activity mitochondrion, cytoplasm 21041 chr16_1 - - 402 E 3 YPL255W BBP1 - Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p cytoskeleton organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 21037 chr16_1 - - 402 E 4 YPL266W DIM1 CDH1 Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing rRNA processing, RNA modification methyltransferase activity nucleus, nucleolus 21026 chr16_1 - - 402 E 5 YPL093W NOG1 - Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins rRNA processing, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport other nucleus, nucleolus 22159 chr16_2 - - 402 E 6 YPL094C SEC62 LPG14 Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER protein targeting, transmembrane transport protein transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 22158 chr16_2 - - 402 E 7 YPL117C IDI1 LPH10|BOT2 Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability lipid metabolic process isomerase activity nucleus, cytoplasm 22135 chr16_2 - - 402 E 8 YPL122C TFB2 - Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH response to DNA damage stimulus, DNA repair, protein phosphorylation, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus 22130 chr16_2 - - 402 E 9 YPL124W SPC29 NIP29|LPH3 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication cytoskeleton organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 22128 chr16_2 - - 402 E 10 YPL126W NAN1 UTP17 U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA transcription from RNA polymerase I promoter, rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus, chromosome 22126 chr16_2 - - 402 E 11 YPL128C TBF1 LPI16 Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation telomere organization, DNA replication, regulation of organelle organization, regulation of DNA metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 22124 chr16_2 - - 402 E 12 YPL142C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit biological_process molecular_function cellular_component 22110 chr16_2 - - 402 F 1 YPL143W RPL33A rp47|l37A|YL37|L33A|RPL37A N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Bp and has similarity to rat L35a; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 22109 chr16_2 - - 402 F 2 YPL151C PRP46 NTC50 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs RNA splicing, mRNA processing molecular_function nucleus 22101 chr16_2 - - 402 F 3 YPL153C RAD53 SPK1|MEC2|LSD1 Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication DNA repair, DNA replication, response to DNA damage stimulus, nucleobase-containing small molecule metabolic process, regulation of cell cycle kinase activity, DNA binding nucleus 22099 chr16_2 - - 402 F 4 YPL160W CDC60 LeuRS|leucyl-tRNA synthetase Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA tRNA aminoacylation for protein translation, DNA replication, cellular amino acid metabolic process ligase activity cytoplasm 22092 chr16_2 - - 402 F 5 YPL169C MEX67 - Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport RNA binding, structural molecule activity nucleus, endomembrane system, membrane, cytoplasm 22083 chr16_2 - - 402 F 6 YPL175W SPT14 GPI3|CWH6 UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins lipid metabolic process, protein lipidation transferase activity, transferring glycosyl groups endoplasmic reticulum, cytoplasm 22077 chr16_2 - - 402 F 7 YBR190W - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W biological_process molecular_function cellular_component 23330 chr2_4 - - 402 F 8 YBR192W RIM2 PYT1 Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family mitochondrion organization, transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, mitochondrion, cytoplasm 23332 chr2_4 - - 402 F 9 YBR193C MED8 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription from RNA polymerase II promoter protein binding transcription factor activity, structural molecule activity, DNA binding, transcription factor binding nucleus 23333 chr2_4 - - 402 F 10 YBR196C PGI1 CDC30 Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, nucleobase-containing small molecule metabolic process isomerase activity membrane fraction, mitochondrion, cytoplasm 23336 chr2_4 - - 402 F 11 YBR198C TAF5 TafII90|TAF90 Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, small conjugating protein binding, structural molecule activity nucleus 23338 chr2_4 - - 402 F 12 YBR202W MCM7 CDC47 Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding, ATPase activity, helicase activity nucleus, chromosome, cytoplasm 23342 chr2_4 - - 402 G 1 YBR211C AME1 ARP100 Essential kinetochore protein associated with microtubules and spindle pole bodies; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance cytoskeleton organization, chromosome segregation molecular_function nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 23351 chr2_4 - - 402 G 2 YBR234C ARC40 - Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches organelle inheritance, mitochondrion organization, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis small conjugating protein binding cytoskeleton 23374 chr2_4 - - 402 G 3 YBR236C ABD1 - Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA mRNA processing, transcription from RNA polymerase II promoter methyltransferase activity nucleus 23376 chr2_4 - - 402 G 4 YBR237W PRP5 RNA5 RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA RNA splicing, mRNA processing ATPase activity nucleus 23377 chr2_4 - - 402 G 5 YBR243C ALG7 TUR1 UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin carbohydrate metabolic process, generation of precursor metabolites and energy, cellular respiration, protein glycosylation other endomembrane system, membrane, endoplasmic reticulum, cytoplasm 23383 chr2_4 - - 402 G 6 YBR247C ENP1 MEG1 Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus, cytoplasm 23387 chr2_4 - - 402 G 7 YBR252W DUT1 - deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate nucleobase-containing small molecule metabolic process other nucleus, cytoplasm 23392 chr2_4 - - 402 G 8 YBR253W SRB6 MED22 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription from RNA polymerase II promoter molecular_function nucleus 23393 chr2_4 met- did not mate 402 G 9 YBR254C TRS20 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Golgi vesicle transport molecular_function cytoplasm, Golgi apparatus 23394 chr2_4 - - 402 G 10 YBR256C RIB5 - Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway vitamin metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups other 23396 chr2_4 - - 402 G 11 YBR257W POP4 - Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P snoRNA processing, tRNA processing, rRNA processing, mRNA processing nuclease activity nucleus, nucleolus 23397 chr2_4 - - 402 G 12 YBR265W TSC10 - 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family lipid metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 23405 chr2_4 - - 402 H 1 YBR189W RPS9B rp21|YS11|S9B|S13|SUP46|RPS13A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins regulation of translation, rRNA processing, translational elongation, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 23329 chr2_4 - - 402 H 2 YBR191W RPL21A L21A|URP1 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 23331 chr2_4 - - 402 H 3 YDR052C DBF4 DNA52|LSD7 Regulatory subunit of Cdc7p-Dbf4p kinase complex, required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated mitotic cell cycle, protein phosphorylation, DNA replication, regulation of protein modification process, regulation of cell cycle enzyme regulator activity, DNA binding other 23988 chr4_4 - - 402 H 4 YDR053W - - Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex biological_process molecular_function membrane 23989 chr4_4 - - 402 H 6 YDR054C CDC34 UBC3|DNA6 Ubiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation mitotic cell cycle, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus, cytoplasm 23990 chr4_4 - - 402 H 7 YDR060W MAK21 NOC1 Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus, cytoplasm 23995 chr4_4 - - 402 H 8 YDR062W LCB2 TSC1|SCS1 Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine lipid metabolic process transferase activity, transferring acyl groups membrane, membrane fraction, endomembrane system, endoplasmic reticulum, cytoplasm 23997 chr4_4 - - 402 H 9 YDR064W RPS13 YS15|S27a|S13|RPS13C|RPS13B Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis RNA binding, structural constituent of ribosome, rRNA binding, structural molecule activity ribosome, cytoplasm 23999 chr4_4 - - 402 H 10 YDR081C PDC2 - Putative transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions carbohydrate metabolic process, vitamin metabolic process, transcription from RNA polymerase II promoter, generation of precursor metabolites and energy molecular_function nucleus 24016 chr4_4 - - 402 H 11 YDR082W STN1 - Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping telomere organization, DNA replication, regulation of organelle organization, regulation of DNA metabolic process DNA binding nucleus, chromosome 24017 chr4_4 - - 402 H 12 YDR086C SSS1 - Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p protein targeting, transmembrane transport protein transporter activity, transmembrane transporter activity, structural molecule activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 24021 chr4_4 - - 403 A 1 YDR087C RRP1 - Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles rRNA processing molecular_function nucleus, nucleolus 24022 chr4_4 - - 403 A 2 YDR088C SLU7 SLT17 RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain RNA splicing, mRNA processing other nucleus 24023 chr4_4 met- did not mate 403 A 4 YDR091C RLI1 - Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase translational initiation, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport ion binding, ATPase activity nucleus, ribosome, cytoplasm 24026 chr4_4 - - 403 A 5 YDR113C PDS1 - Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, chromosome segregation, response to DNA damage stimulus, DNA recombination enzyme binding nucleus, cytoskeleton, cytoplasm 24047 chr4_4 - - 403 A 6 YDR118W APC4 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus 24052 chr4_4 - - 403 A 7 YDR141C DOP1 - Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Golgi vesicle transport, cell morphogenesis, endosome transport molecular_function mitochondrion, cytoplasm, Golgi apparatus 24075 chr4_4 - - 403 A 8 YDR145W TAF12 TafII61|TafII68|TAF68|TAF61 Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, structural molecule activity, transcription factor binding nucleus 24079 chr4_4 met- did not mate 403 A 9 YDR339C FCF1 UTP24 Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 24178 chr4_7 - - 403 A 10 YDR341C - ArgRS|RRS1 Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cytoplasmic translation, tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 24180 chr4_7 - - 403 A 11 YDR353W TRR1 - Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress response to oxidative stress, response to chemical stimulus ion binding, oxidoreductase activity cytoplasm 24190 chr4_7 - - 403 A 12 YDR355C - - Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W biological_process molecular_function cellular_component 24192 chr4_7 - - 403 B 1 YDR356W SPC110 NUF1|XCM1 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner cytoskeleton organization, conjugation, organelle fusion, nucleus organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 24193 chr4_7 - - 403 B 2 YDR361C BCP1 - Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport protein targeting, ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, cytoplasm 24198 chr4_7 met- did not mate 403 B 3 YDR362C TFC6 tau 91 One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 transcription from RNA polymerase III promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus 24199 chr4_7 - - 403 B 4 YDR365C ESF1 - Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels rRNA processing RNA binding nucleus, nucleolus 24202 chr4_7 - - 403 B 5 YDR367W KEI1 - Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene lipid metabolic process enzyme regulator activity membrane, endomembrane system, cytoplasm, Golgi apparatus 24203 chr4_7 - - 403 B 6 YDR373W FRQ1 - N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily signaling ion binding, enzyme regulator activity membrane, endomembrane system, cytoplasm, Golgi apparatus 24209 chr4_7 - - 403 B 7 YDR376W ARH1 - Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability cofactor metabolic process, cellular ion homeostasis oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 24212 chr4_7 - - 403 B 8 YDR381W YRA1 SHE11 RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member response to DNA damage stimulus, DNA repair, nucleobase-containing compound transport, nuclear transport RNA binding nucleus 24217 chr4_7 - - 403 B 9 YDR390C UBA2 UAL1 Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein modification by small protein conjugation or removal other nucleus 24226 chr4_7 - - 403 B 10 YDR394W RPT3 YTA2|YNT1 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, proteolysis involved in cellular protein catabolic process, protein complex biogenesis ATPase activity other 24230 chr4_7 - - 403 B 11 YDR396W - - Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex biological_process molecular_function cellular_component 24232 chr4_7 - - 403 B 12 YDR397C NCB2 YDR1 Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 24233 chr4_7 - - 403 C 1 YDR398W UTP5 - Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit transcription from RNA polymerase I promoter, rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus, chromosome 24234 chr4_7 - - 403 C 2 YDR404C RPB7 B16 RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation translational initiation, regulation of translation, RNA catabolic process, transcription initiation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter RNA binding, nucleotidyltransferase activity, DNA binding nucleus, cytoplasm 24240 chr4_7 - - 403 C 3 YDR407C TRS120 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Golgi vesicle transport, endosome transport, nucleobase-containing small molecule metabolic process, signaling enzyme regulator activity cytoplasm, Golgi apparatus 24243 chr4_7 - - 403 C 4 YDR412W RRP17 - Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA rRNA processing molecular_function cytoplasm 24248 chr4_7 - - 403 C 5 YDR413C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing biological_process molecular_function membrane 24249 chr4_7 met- did not mate 403 C 6 YDR416W SYF1 NTC90 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans RNA splicing, mRNA processing other nucleus 24252 chr4_7 - - 403 C 7 YDR429C TIF35 - eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation cytoplasmic translation, translational initiation RNA binding, translation factor activity, nucleic acid binding cytoplasm 24265 chr4_7 - - 403 C 8 YDR434W GPI17 - Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog lipid metabolic process, protein lipidation other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 24270 chr4_7 - - 403 C 9 YDR427W RPN9 - Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects proteolysis involved in cellular protein catabolic process, protein complex biogenesis structural molecule activity cytoplasm 24263 chr4_7 - - 403 C 10 YEL002C WBP1 - Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum carbohydrate metabolic process, protein glycosylation transferase activity, transferring glycosyl groups nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 20242 chr5_2 - - 403 C 11 YEL019C MMS21 PSO10|NSE2 SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination response to DNA damage stimulus, DNA repair ligase activity nucleus, endomembrane system, chromosome, cytoplasm 20260 chr5_2 - - 403 C 12 YEL026W SNU13 - RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein RNA splicing, rRNA processing, mRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 20267 chr5_2 - - 403 D 1 YEL032W MCM3 - Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity nucleus, chromosome, cytoplasm 20273 chr5_2 - - 403 D 2 YEL035C UTR5 - Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions biological_process molecular_function cellular_component 20276 chr5_2 - - 403 D 3 YEL055C POL5 - DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA other nucleotidyltransferase activity nucleus, nucleolus 20296 chr5_2 - - 403 D 4 YEL058W PCM1 AGM1 Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors carbohydrate metabolic process, cell wall organization or biogenesis isomerase activity nucleus, cytoplasm 20299 chr5_2 - - 403 D 5 YER003C PMI40 PMI Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation protein glycosylation, carbohydrate metabolic process, cell wall organization or biogenesis isomerase activity nucleus, cytoplasm 20324 chr5_2 - - 403 D 6 YER006W NUG1 - GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus rRNA processing, ribosomal subunit export from nucleus, nuclear transport RNA binding, GTPase activity nucleus, nucleolus 20327 chr5_2 - - 403 D 7 YER009W NTF2 - Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport protein targeting, nuclear transport enzyme binding nucleus, membrane, endomembrane system 20331 chr5_2 - - 403 D 8 YER012W PRE1 - Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle proteolysis involved in cellular protein catabolic process enzyme regulator activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 20334 chr5_2 - - 403 D 9 YER013W PRP22 - DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes RNA splicing, mRNA processing helicase activity, ATPase activity nucleus 20335 chr5_2 - - 403 D 10 YER021W RPN3 SUN2 Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control proteolysis involved in cellular protein catabolic process molecular_function other 20153 chr5_3 - - 403 D 11 YER022W SRB4 MED17 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, structural molecule activity, transcription factor binding nucleus 20154 chr5_3 - - 403 D 12 YER023W PRO3 ORE2 Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis cellular amino acid metabolic process oxidoreductase activity cytoplasm 20155 chr5_3 - - 403 E 1 YER025W GCD11 SUI4 Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met regulation of translation, translational elongation RNA binding, translation factor activity, nucleic acid binding ribosome, cytoplasm 20157 chr5_3 - - 403 E 2 YER036C ARB1 - ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p ribosomal subunit export from nucleus, nuclear transport ATPase activity nucleus, ribosome, cytoplasm 20168 chr5_3 - - 403 E 3 YER038C KRE29 NSE6 Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance response to DNA damage stimulus, DNA repair molecular_function nucleus, chromosome, cytoplasm 20170 chr5_3 met- did not mate 403 E 4 YER043C SAH1 - S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor cellular amino acid metabolic process other cytoplasm 20176 chr5_3 - - 403 E 5 YER082C UTP7 KRE31 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 20222 chr5_3 - - 403 E 6 YGR128C UTP8 - Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA transcription from RNA polymerase I promoter, rRNA processing, nucleobase-containing compound transport, nuclear transport, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus, chromosome 24758 chr7_5 - - 403 E 7 YGR140W CBF2 CSL5|NDC10|CTF14|CEP2|CBF3A Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo mitotic cell cycle, cytoskeleton organization, protein complex biogenesis, chromosome segregation DNA binding nucleus, cytoskeleton, chromosome 24770 chr7_5 - - 403 E 8 YGR147C NAT2 - Protein with an apparent role in acetylation of N-terminal methionine residues peptidyl-amino acid modification, protein acylation molecular_function mitochondrion, cytoplasm 24777 chr7_5 - - 403 E 9 YGR156W PTI1 - Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 snoRNA processing, transcription termination, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter RNA binding nucleus 24786 chr7_5 - - 403 E 10 YGR158C MTR3 - Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) RNA catabolic process, snoRNA processing, rRNA processing molecular_function nucleus, nucleolus, cytoplasm 24788 chr7_5 - - 403 E 11 YGR172C YIP1 - Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 24802 chr7_5 - - 403 E 12 YGR175C ERG1 - Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine lipid metabolic process oxidoreductase activity endoplasmic reticulum, cytoplasm 24805 chr7_5 - - 403 F 1 YGR179C OKP1 - Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly cytoskeleton organization, chromosome segregation molecular_function nucleus, chromosome 24809 chr7_5 - - 403 F 2 YGR185C TYS1 TyrRS|TTS1 Cytoplasmic tyrosyl-tRNA synthetase, required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity nucleus, cytoplasm 24815 chr7_5 - - 403 F 3 YGR186W TFG1 RAP74|SSU71 TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 protein dephosphorylation, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, enzyme regulator activity nucleus 24816 chr7_5 - - 403 F 4 YGR190C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W biological_process molecular_function cellular_component 24820 chr7_5 - - 403 F 5 YGR191W HIP1 - High-affinity histidine permease, also involved in the transport of manganese ions ion transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 24821 chr7_5 - - 403 F 6 YGR195W SKI6 RRP41|ECM20 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) RNA catabolic process, snoRNA processing, rRNA processing molecular_function nucleus, cytoplasm 24825 chr7_5 - - 403 F 7 YGR198W YPP1 - Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene protein targeting, response to chemical stimulus, membrane invagination, endocytosis molecular_function cell cortex, cytoskeleton, ribosome, membrane, cytoplasm, plasma membrane 24828 chr7_5 - - 403 F 8 YGR211W ZPR1 - Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p) mitotic cell cycle, response to starvation, regulation of cell cycle other nucleus, cytoplasm 24841 chr7_5 - - 403 F 9 YGR216C GPI1 - Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs lipid metabolic process, protein lipidation transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 24846 chr7_5 - - 403 F 10 YGR218W CRM1 XPO1|KAP124 Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin protein targeting, ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport protein transporter activity nucleus, cytoskeleton, microtubule organizing center, chromosome, membrane, cytoplasm 24848 chr7_5 - - 403 F 11 YHR019C DED81 asparaginyl-tRNA synthetase Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity cytoplasm 20982 chr8_1 - - 403 F 12 YHR024C MAS2 mitochondrial processing protease alpha subunit|MIF2 Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins protein targeting, mitochondrion organization, protein maturation peptidase activity mitochondrion, cytoplasm 20987 chr8_1 - - 403 G 1 YHR036W BRL1 - Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export protein targeting, nucleobase-containing compound transport, nuclear transport, nucleus organization molecular_function nucleus, endomembrane system, endoplasmic reticulum, cytoplasm 20999 chr8_1 - - 403 G 2 YHR040W BCD1 - Essential protein required for the accumulation of box C/D snoRNA other other nucleus, cytoplasm 21003 chr8_1 met- did not mate 403 G 3 YHR042W NCP1 CPR1 NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p lipid metabolic process other membrane, membrane fraction, mitochondrial envelope, mitochondrion, cytoplasm 21005 chr8_1 - - 403 G 4 YHR062C RPP1 - Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs snoRNA processing, tRNA processing, rRNA processing, mRNA processing nuclease activity nucleus, nucleolus 21890 chr8_2 - - 403 G 5 YHR065C RRP3 - Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity rRNA processing, ribosomal small subunit biogenesis helicase activity, ATPase activity nucleus, nucleolus 21893 chr8_2 - - 403 G 6 YHR068W DYS1 - Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric peptidyl-amino acid modification, cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 21896 chr8_2 - - 403 G 7 YHR069C RRP4 - Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) RNA catabolic process, snoRNA processing, rRNA processing molecular_function nucleus, cytoplasm 21897 chr8_2 - - 403 G 8 YHR070W TRM5 - tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya tRNA processing, RNA modification methyltransferase activity nucleus, mitochondrion, cytoplasm 21898 chr8_2 - - 403 G 9 YHR072W ERG7 - Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis lipid metabolic process isomerase activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 21900 chr8_2 - - 403 G 10 YHR074W QNS1 - Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ligase activity nucleus, cytoplasm 21902 chr8_2 - - 403 G 11 YHR083W SAM35 TOM38|TOB38|FMP20 Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane protein targeting, mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 21911 chr8_2 - - 403 G 12 YHR085W IPI1 - Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus 21913 chr8_2 - - 403 H 1 YHR088W RPF1 - Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA rRNA processing, organelle assembly, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, ribosome assembly, nuclear transport RNA binding, rRNA binding nucleus, nucleolus 21916 chr8_2 - - 403 H 2 YHR101C BIG1 - Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan cell wall organization or biogenesis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 21929 chr8_2 - - 403 H 3 YHR107C CDC12 PSL7|CLA10 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells protein phosphorylation, cytoskeleton organization, cytokinesis, protein complex biogenesis, regulation of protein modification process GTPase activity, lipid binding, structural molecule activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 21935 chr8_2 - - 403 H 4 YHR118C ORC6 - Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p DNA replication DNA binding nucleus, chromosome 21946 chr8_2 - - 403 H 6 YHR122W - - Protein of unknown function required for establishment of sister chromatid cohesion; synthetically lethal with RFC5, an RF-C subunit that links replication to cohesion establishment; YHR122W is an essential gene mitotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, cytoplasm 21950 chr8_2 - - 403 H 7 YHR084W STE12 - Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth invasive growth in response to glucose limitation, conjugation, pseudohyphal growth, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 21912 chr8_2 - - 403 H 8 YHR143W-A RPC10 ABC10-alpha|RPB12 RNA polymerase subunit ABC10-alpha, found in RNA polymerase complexes I, II, and III transcription from RNA polymerase I promoter, transcription from RNA polymerase III promoter, transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus, nucleolus 22837 chr8_3 - - 403 H 9 YHR148W IMP3 - Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA rRNA processing RNA binding nucleus, nucleolus 22842 chr8_3 - - 403 H 10 YHR164C DNA2 WEB2 Tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization meiotic cell cycle, DNA repair, telomere organization, DNA replication, response to DNA damage stimulus DNA binding, ATPase activity, helicase activity, nuclease activity nucleus, chromosome, cytoplasm 22858 chr8_3 - - 403 H 11 YHR166C CDC23 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus 22860 chr8_3 met- did not mate 403 H 12 YHR172W SPC97 - Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque cytoskeleton organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 22866 chr8_3 - - 404 A 1 YHR186C KOG1 LAS24 Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors response to starvation, signaling small conjugating protein binding membrane fraction, vacuole, mitochondrion, membrane, cytoplasm, plasma membrane 22880 chr8_3 - - 404 A 2 YHR188C GPI16 - Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog lipid metabolic process, protein lipidation other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 22882 chr8_3 - - 404 A 3 YHR190W ERG9 - Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway lipid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups membrane fraction, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 22884 chr8_3 - - 404 A 5 YHR196W UTP9 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA transcription from RNA polymerase I promoter, rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus, chromosome 22890 chr8_3 - - 404 A 6 YHR197W RIX1 IPI2 Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles ribosome assembly, rRNA processing, organelle assembly, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport molecular_function nucleus 22891 chr8_3 - - 404 A 7 YCL003W - - - - - - 23411 chr3_1 - - 404 A 8 YCL004W PGS1 YCL003W|PEL1 Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis lipid metabolic process other mitochondrion, cytoplasm 23412 chr3_1 - - 404 A 9 YCL017C NFS1 SPL1 Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria cofactor metabolic process, cellular ion homeostasis, tRNA processing, mitochondrion organization, nucleobase-containing small molecule metabolic process, RNA modification other nucleus, mitochondrion, cytoplasm 23424 chr3_1 - - 404 A 10 YCL031C RRP7 - Essential protein involved in rRNA processing and ribosome biogenesis ribosome assembly, rRNA processing, organelle assembly, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 23438 chr3_1 - - 404 A 11 YCL043C PDI1 TRG1|MFP1 Protein disulfide isomerase; multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; forms a complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2 which promotes degradation in the unfolded protein response protein folding isomerase activity, oxidoreductase activity endoplasmic reticulum, cytoplasm 23450 chr3_1 - - 404 A 12 YCL052C PBN1 - Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X lipid metabolic process, proteolysis involved in cellular protein catabolic process, protein maturation, protein lipidation transferase activity, transferring glycosyl groups endoplasmic reticulum, cytoplasm 23459 chr3_1 - - 404 B 1 YCL054W SPB1 - AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants rRNA processing, ribosomal large subunit biogenesis, RNA modification methyltransferase activity nucleus, nucleolus 23461 chr3_1 - - 404 B 2 YCL059C KRR1 - Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 23466 chr3_1 met- did not mate 404 B 3 YCR012W PGK1 - 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis carbohydrate metabolic process, generation of precursor metabolites and energy kinase activity membrane fraction, mitochondrion, cytoplasm 23492 chr3_1 - - 404 B 4 YCR013C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant biological_process molecular_function cellular_component 23493 chr3_1 - - 404 B 5 YLR127C APC2 TID2|RSI1 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p mitotic cell cycle, organelle fission, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus 24084 chr12_3 - - 404 B 6 YLR129W DIP2 UTP12 Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 24086 chr12_3 - - 404 B 7 YLR132C USB1 - Essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants biological_process molecular_function nucleus, mitochondrion, cytoplasm 24089 chr12_3 - - 404 B 8 YLR140W - - Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I biological_process molecular_function cellular_component 24097 chr12_3 - - 404 B 9 YLR141W RRN5 - Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p transcription from RNA polymerase I promoter, chromatin organization nucleic acid binding transcription factor activity nucleus, nucleolus 24098 chr12_3 - - 404 B 10 YLR145W RMP1 - Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P rRNA processing, mRNA processing nuclease activity nucleus, nucleolus, cytoplasm 24102 chr12_3 - - 404 B 11 YLR147C SMD3 Sm D3|SLT16 Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 24104 chr12_3 - - 404 B 12 YLR163C MAS1 mitochondrial processing protease beta subunit|MIF1 Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins protein targeting, mitochondrion organization, protein maturation peptidase activity mitochondrion, cytoplasm 24112 chr12_3 - - 404 C 1 YLR166C SEC10 - Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis cell budding, cytokinesis, organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, exocytosis molecular_function cell cortex, site of polarized growth, cytoplasm 24115 chr12_3 - - 404 C 2 YLR167W RPS31 YS24|S37|S31|UBI3|RPS37 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 24116 chr12_3 - - 404 C 3 YLR175W CBF5 - Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita snoRNA processing, rRNA processing, RNA modification isomerase activity nucleus, nucleolus 24124 chr12_3 - - 404 C 4 YLR186W EMG1 NEP1 Member of the alpha/beta knot fold methyltransferase superfamily; required for maturation of 18S rRNA and for 40S ribosome production; interacts with RNA and with S-adenosylmethionine; associates with spindle/microtubules; forms homodimers rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus, cytoskeleton, cytoplasm 24135 chr12_3 - - 404 C 5 YLR195C NMT1 CDC72 N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction peptidyl-amino acid modification, protein acylation, protein lipidation transferase activity, transferring acyl groups cytoplasm 24144 chr12_3 - - 404 C 6 YLR196W PWP1 - Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily rRNA processing molecular_function nucleus, nucleolus, cytoplasm 24145 chr12_3 - - 404 C 7 YLR197W NOP56 SIK1 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects rRNA processing, RNA modification molecular_function nucleus, nucleolus 24146 chr12_3 - - 404 C 8 YLR198C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W biological_process molecular_function cellular_component 24147 chr12_3 - - 404 C 9 YLR208W SEC13 ANU3 Component of the Nup84 nuclear pore sub-complex, the Sec13p-Sec31p complex of the COPII vesicle coat, and the SEA (Seh1-associated) complex; required for vesicle formation in ER to Golgi transport and nuclear pore complex organization; the Nup84 subcomplex has a role in transcription elongation vesicle organization, Golgi vesicle transport, nucleobase-containing small molecule metabolic process, nucleus organization structural molecule activity nucleus, endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm 24157 chr12_3 - - 404 C 10 YLR212C TUB4 - Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body mitotic cell cycle, cytoskeleton organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 24161 chr12_3 - - 404 C 11 YLR222C UTP13 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 24171 chr12_3 - - 404 C 12 YLR223C IFH1 - Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing transcription from RNA polymerase III promoter, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity nucleus, nucleolus, chromosome 24172 chr12_3 - - 404 D 1 YKL004W AUR1 - Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance lipid metabolic process transferase activity, transferring glycosyl groups membrane, cytoplasm, Golgi apparatus 24852 chr11_1 - - 404 D 2 YKL006C-A SFT1 - Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment membrane fusion, Golgi vesicle transport, organelle fusion, vesicle organization other membrane, endomembrane system, cytoplasm, Golgi apparatus 24854 chr11_1 - - 404 D 3 YKL012W PRP40 - U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex RNA splicing, mRNA processing RNA binding nucleus 24861 chr11_1 - - 404 D 4 YKL013C ARC19 - Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches organelle inheritance, mitochondrion organization, cytoskeleton organization structural molecule activity cytoskeleton 24862 chr11_1 - - 404 D 5 YKL014C URB1 NPA1 Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit rRNA processing, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 24863 chr11_1 - - 404 D 6 YKL018W SWD2 SAF37|CPS35 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination telomere organization, snoRNA processing, protein alkylation, chromatin organization, histone modification, transcription termination, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter methyltransferase activity nucleus 24867 chr11_1 - - 404 D 7 YKL019W RAM2 - Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor protein maturation transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 24868 chr11_1 - - 404 D 8 YKL021C MAK11 - Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis molecular_function membrane fraction 24870 chr11_1 - - 404 D 9 YKL022C CDC16 - Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation DNA replication, protein modification by small protein conjugation or removal, regulation of DNA metabolic process, proteolysis involved in cellular protein catabolic process ligase activity nucleus 24871 chr11_1 - - 404 D 10 YKL024C URA6 SOC8 Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) nucleobase-containing small molecule metabolic process kinase activity nucleus, cytoplasm 24873 chr11_1 - - 404 D 11 YKL028W TFA1 - TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter enzyme binding, DNA binding, transcription factor binding nucleus, mitochondrion, cytoplasm 24877 chr11_1 - - 404 D 12 YKL033W TTI1 FMP47 Putative protein of unknown function; subunit of the ASTRA complex which is part of the chromatin remodeling machinery; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies biological_process molecular_function nucleus, chromosome, mitochondrion, cytoplasm 24882 chr11_1 - - 404 E 1 YKL035W UGP1 - UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p carbohydrate metabolic process, generation of precursor metabolites and energy, oligosaccharide metabolic process nucleotidyltransferase activity membrane fraction, cytoplasm 24884 chr11_1 - - 404 E 2 YKL036C - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W biological_process molecular_function cellular_component 24885 chr11_1 - - 404 E 3 YKL042W SPC42 - Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane cytoskeleton organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 24891 chr11_1 - - 404 E 4 YKL045W PRI2 - Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair DNA repair, DNA replication, response to DNA damage stimulus nucleotidyltransferase activity, DNA binding nucleus, endomembrane system, chromosome 24894 chr11_1 - - 404 E 5 YKL049C CSE4 CSL2 Centromere protein that resembles histone H3, required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p mitotic cell cycle, organelle fission, chromosome segregation DNA binding nucleus, chromosome 24898 chr11_1 - - 404 E 6 YKL052C ASK1 - Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, chromosome 24901 chr11_1 - - 404 E 7 YKL058W TOA2 - TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 24907 chr11_1 - - 404 E 8 YKL059C MPE1 - Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif mRNA processing RNA binding nucleus 24908 chr11_1 - - 404 E 9 YKL060C FBA1 LOT1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress carbohydrate metabolic process, generation of precursor metabolites and energy lyase activity mitochondrion, cytoplasm 24909 chr11_1 - - 404 E 10 YKL078W DHR2 - Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis rRNA processing, ribosomal small subunit biogenesis helicase activity nucleus, nucleolus 24927 chr11_1 - - 404 E 11 YKL083W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 biological_process molecular_function membrane 24932 chr11_1 - - 404 E 12 YKL088W CAB3 - Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to osmotic stress lyase activity cytoplasm 24937 chr11_1 - - 404 F 1 YKL089W MIF2 - Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p mitotic cell cycle, cytoskeleton organization, chromosome segregation DNA binding nucleus, chromosome 24938 chr11_1 - - 404 F 2 YKL095W YJU2 CWC16 Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing RNA splicing, mRNA processing other nucleus 24944 chr11_1 met- did not mate 404 F 3 YKL099C UTP11 - Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 24949 chr11_2 - - 404 F 4 YKL104C GFA1 - Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis carbohydrate metabolic process, cell wall organization or biogenesis other cellular_component 24954 chr11_2 - - 404 F 5 YKL108W SLD2 DRC1 Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination, regulation of cell cycle DNA binding nucleus 24958 chr11_2 - - 404 F 6 YKL111C - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 biological_process molecular_function cellular_component 24961 chr11_2 - - 404 F 7 YKL112W ABF1 SBF1|REB2|OBF1|BAF1 DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair response to DNA damage stimulus, DNA repair, chromatin organization, DNA replication, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome 24962 chr11_2 - - 404 F 8 YKL122C SRP21 - Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm protein targeting, protein complex biogenesis other nucleus, cytoplasm 24972 chr11_2 - - 404 F 9 YKL125W RRN3 - Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo transcription from RNA polymerase I promoter, transcription initiation, DNA-dependent enzyme binding, protein binding transcription factor activity, transcription factor binding nucleus, nucleolus 24975 chr11_2 - - 404 F 10 YKL144C RPC25 C25|YKL1 RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p transcription initiation, DNA-dependent, transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus 24994 chr11_2 - - 404 F 11 YKL145W RPT1 YTA3|CIM5 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, proteolysis involved in cellular protein catabolic process, protein complex biogenesis ATPase activity other 24995 chr11_2 - - 404 F 12 YKL152C GPM1 - Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis carbohydrate metabolic process, generation of precursor metabolites and energy isomerase activity mitochondrion, cytoplasm 25002 chr11_2 - - 404 G 1 YKL153W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant biological_process molecular_function cellular_component 25003 chr11_2 - - 404 G 2 YKL154W SRP102 - Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane protein targeting GTPase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 25004 chr11_2 - - 404 G 3 YKL165C MCD4 FSR2|SSU21|ZRG16 Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes transmembrane transport, nucleobase-containing compound transport, lipid metabolic process, protein lipidation other vacuole, cell wall, membrane, endoplasmic reticulum, cytoplasm 25015 chr11_2 - - 404 G 4 YKL172W EBP2 - Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering organelle fission, rRNA processing molecular_function nucleus, nucleolus 25022 chr11_2 - - 404 G 5 YKL173W SNU114 GIN10 GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 RNA splicing, mRNA processing RNA binding, GTPase activity nucleus 25023 chr11_2 - - 404 G 6 YKL180W RPL17A YL17|L20A|L17A|RPL17 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the Dam1 complex (aka DASH complex) cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 25030 chr11_2 - - 404 G 7 YKL186C MTR2 - mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport other nucleus, membrane, endomembrane system 25036 chr11_2 - - 404 G 8 YKL189W HYM1 - Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response cell morphogenesis, cell budding, cytokinesis molecular_function cellular bud, site of polarized growth 25039 chr11_2 - - 404 G 9 YKL139W CTK1 - Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 transcription from RNA polymerase I promoter, peptidyl-amino acid modification, regulation of translation, protein phosphorylation, translational elongation, mRNA processing, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter kinase activity nucleus, nucleolus 24989 chr11_2 - - 404 G 10 YKL141W SDH3 YKL4|CYB3 Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex protein targeting, cofactor metabolic process, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 24991 chr11_2 - - 404 G 11 YKL181W PRS1 PRP1 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes carbohydrate metabolic process, cell wall organization or biogenesis other cytoplasm 25031 chr11_2 - - 404 G 12 YKL182W FAS1 - Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities lipid metabolic process lyase activity, transferase activity, transferring acyl groups, oxidoreductase activity mitochondrion, cytoplasm 25032 chr11_2 - - 404 H 1 YGR029W ERV1 - Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) protein targeting, mitochondrion organization, transmembrane transport, response to oxidative stress, response to chemical stimulus, cellular ion homeostasis oxidoreductase activity mitochondrion, mitochondrial envelope, cytoplasm 24659 chr7_4 - - 404 H 2 YGR030C POP6 - Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs snoRNA processing, tRNA processing, rRNA processing, mRNA processing nuclease activity nucleus, nucleolus 24660 chr7_4 - - 404 H 3 YGR047C TFC4 tau 131|PCF1 One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 transcription from RNA polymerase III promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus 24677 chr7_4 - - 404 H 4 YGR060W ERG25 - C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 24690 chr7_4 - - 404 H 6 YGR065C VHT1 - High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin transmembrane transport transmembrane transporter activity membrane, plasma membrane 24695 chr7_4 - - 404 H 7 YGR073C - - Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein biological_process molecular_function cellular_component 24703 chr7_4 - - 404 H 8 YGR074W SMD1 Sm D1|SPP92 Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1 RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 24704 chr7_4 - - 404 H 9 YGR075C PRP38 - Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly RNA splicing, mRNA processing molecular_function nucleus 24705 chr7_4 - - 404 H 10 YGR083C GCD2 GCD12 Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression translational initiation, regulation of translation RNA binding, translation factor activity, nucleic acid binding, enzyme regulator activity cytoplasm 24713 chr7_4 met- did not mate 404 H 11 YGR090W UTP22 - Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 24720 chr7_4 - - 404 H 12 YGR091W PRP31 - Splicing factor, component of the U4/U6-U5 snRNP complex RNA splicing, mRNA processing molecular_function nucleus, mitochondrion, cytoplasm 24721 chr7_4 - - 405 A 1 YGR094W VAS1 - Mitochondrial and cytoplasmic valyl-tRNA synthetase tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 24724 chr7_4 - - 405 A 2 YGR095C RRP46 - Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) RNA catabolic process, snoRNA processing, rRNA processing molecular_function nucleus, cytoplasm 24725 chr7_4 - - 405 A 3 YGR098C ESP1 - Separase, a caspase-like cysteine protease that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle peptidase activity nucleus, cytoskeleton, mitochondrion, cytoplasm 24728 chr7_4 - - 405 A 4 YGR099W TEL2 - Essential DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect telomere organization, DNA replication DNA binding nucleus, chromosome 24729 chr7_4 - - 405 A 6 YGR103W NOP7 YPH1 Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation rRNA processing, ribosomal large subunit biogenesis, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 24733 chr7_4 - - 405 A 7 YGR113W DAM1 - Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments mitotic cell cycle, cytoskeleton organization, regulation of organelle organization cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, chromosome 24743 chr7_4 - - 405 A 8 YGR114C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 biological_process molecular_function membrane 24744 chr7_4 - - 405 A 9 YGR115C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C biological_process molecular_function membrane 24745 chr7_4 - - 405 A 10 YGR119C NUP57 - Nucleoporin, essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p protein targeting, nuclear transport, nucleus organization structural molecule activity nucleus, membrane, endomembrane system 24749 chr7_4 - - 405 A 11 YGR120C COG2 SEC35 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting, response to starvation, peroxisome organization molecular_function membrane fraction, cytoplasm, Golgi apparatus 24750 chr7_4 - - 405 A 12 YOR098C NUP1 - Nuclear pore complex (NPC) subunit, involved in protein import/export and in export of RNAs, possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; potential Cdc28p substrate protein targeting, ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport structural molecule activity nucleus, endomembrane system, membrane 22354 chr15_2 - - 405 B 1 YOR102W - - Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex biological_process molecular_function cellular_component 22358 chr15_2 - - 405 B 2 YOR110W TFC7 tau 55 One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes transcription from RNA polymerase III promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus 22366 chr15_2 - - 405 B 3 YOR116C RPO31 C160|RPC160|RPC1 RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus 22372 chr15_2 - - 405 B 4 YOR117W RPT5 YTA1 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, proteolysis involved in cellular protein catabolic process, protein complex biogenesis ATPase activity other 22373 chr15_2 - - 405 B 5 YOR119C RIO1 RRP10 Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA mitotic cell cycle, rRNA processing, ribosomal small subunit biogenesis, regulation of cell cycle kinase activity nucleus, cytoplasm 22375 chr15_2 - - 405 B 6 YOR122C PFY1 PRF1 Profilin, binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress cytoskeleton organization, regulation of organelle organization, protein complex biogenesis cytoskeletal protein binding, lipid binding membrane, plasma membrane, cytoplasm 22378 chr15_2 - - 405 B 7 YOR143C THI80 - Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) cofactor metabolic process, vitamin metabolic process other cytoplasm 22399 chr15_2 - - 405 B 8 YOR145C PNO1 RRP20|DIM2 Essential nucleolar protein required for pre-18S rRNA processing, interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain rRNA processing, ribosomal small subunit biogenesis, protein complex biogenesis unfolded protein binding nucleus, nucleolus 22401 chr15_2 - - 405 B 9 YOR146W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C biological_process molecular_function cellular_component 22402 chr15_2 - - 405 B 10 YOR148C SPP2 - Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction RNA splicing, mRNA processing molecular_function nucleus 22404 chr15_2 - - 405 B 11 YOR149C SMP3 SAP2|LAS2 Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure lipid metabolic process, protein lipidation transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 22405 chr15_2 - - 405 B 12 YOR151C RPB2 B150|SOH2|SIT2|RPO22|RPB150 RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus, mitochondrion, cytoplasm 22407 chr15_2 - - 405 C 1 YJL074C SMC3 - Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member mitotic cell cycle, meiotic cell cycle, organelle fission, sporulation, DNA recombination, chromosome segregation ATPase activity nucleus, chromosome 21349 chr10_2 - - 405 C 2 YOR159C SME1 Sm E|SmE Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E RNA splicing, mRNA processing molecular_function nucleus 22415 chr15_2 - - 405 C 3 YJL076W NET1 SRM8|CFI1|ESC5 Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure mitotic cell cycle, regulation of cell cycle, nucleus organization DNA binding nucleus, nucleolus 21347 chr10_2 met- did not mate 405 C 4 YOR168W GLN4 glutamyl-tRNA synthetase Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 22424 chr15_2 - - 405 C 5 YOR169C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W biological_process molecular_function cellular_component 22425 chr15_2 - - 405 C 6 YOR174W MED4 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription from RNA polymerase II promoter molecular_function nucleus 22430 chr15_2 - - 405 C 7 YOR176W HEM15 - Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway cofactor metabolic process lyase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 22432 chr15_2 - - 405 C 8 YOR181W LAS17 BEE1 Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) cell budding, cytoskeleton organization, regulation of organelle organization, cytokinesis, membrane invagination, response to osmotic stress, endocytosis cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm 22437 chr15_2 - - 405 C 9 YOR103C OST2 - Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 22359 chr15_2 - - 405 C 10 YOR194C TOA1 - TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 22450 chr15_3 - - 405 C 11 YOR203W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation biological_process molecular_function cellular_component 22459 chr15_3 - - 405 C 12 YOR204W DED1 SPP81 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility translational initiation RNA binding, helicase activity, ATPase activity cytoplasm 22460 chr15_3 - - 405 D 1 YOR206W NOC2 - Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors organelle assembly, ribosomal subunit export from nucleus, ribosome assembly, nuclear transport molecular_function nucleus, mitochondrion, nucleolus, cytoplasm 22462 chr15_3 - - 405 D 2 YOR207C RET1 C128|RPC128|RPC2|PDS2 Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus 22463 chr15_3 - - 405 D 3 YOR210W RPB10 ABC10-beta RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III transcription from RNA polymerase I promoter, transcription from RNA polymerase III promoter, transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus, nucleolus 22466 chr15_3 - - 405 D 4 YOR217W RFC1 CDC44 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon response to DNA damage stimulus, mitotic cell cycle, DNA repair, DNA replication DNA binding, ATPase activity chromosome 22473 chr15_3 - - 405 D 5 YOR218C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W biological_process molecular_function cellular_component 22474 chr15_3 met- did not mate 405 D 6 YJL081C ARP4 ACT3 Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, organelle assembly, protein complex biogenesis, histone modification, response to DNA damage stimulus, transcription from RNA polymerase II promoter ATPase activity, helicase activity, transferase activity, transferring acyl groups nucleus, chromosome 21342 chr10_2 lys- did not mate 405 D 7 YOR232W MGE1 GRPE|YGE1 Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE protein targeting, mitochondrion organization, transmembrane transport, protein folding enzyme regulator activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 22488 chr15_3 - - 405 D 8 YOR236W DFR1 - Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function cofactor metabolic process RNA binding, mRNA binding, oxidoreductase activity cytoplasm 22492 chr15_3 - - 405 D 9 YOR244W ESA1 KAT5|TAS1 Catalytic subunit of the histone acetyltransferase complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression and transcriptional silencing at the rDNA locus peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, histone modification, response to DNA damage stimulus, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, regulation of cell cycle transferase activity, transferring acyl groups nucleus 22500 chr15_3 - - 405 D 10 YOR249C APC5 RMC1 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus 22505 chr15_3 - - 405 D 11 YOR250C CLP1 - Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation mRNA processing RNA binding nucleus 22506 chr15_3 - - 405 D 12 YOR254C SEC63 PTL1 Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER protein targeting, transmembrane transport protein transporter activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 22510 chr15_3 - - 405 E 1 YOR256C TRE2 - Protein that functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; has similarity to transferrin receptors; inviability of null mutant in systematic studies is due to proximity to CDC31 protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process molecular_function cellular_component 22512 chr15_3 - - 405 E 2 YOR257W CDC31 DSK1 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation cytoskeleton organization, proteolysis involved in cellular protein catabolic process structural molecule activity nucleus, cytoskeleton, microtubule organizing center, endomembrane system, membrane, cytoplasm 22513 chr15_3 - - 405 E 3 YOR259C RPT4 SUG2|PCS1|CRL13 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization DNA repair, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, response to DNA damage stimulus, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity nucleus 22515 chr15_3 - - 405 E 4 YJL085W EXO70 - Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane targeting of the exocyst via PI(4,5)P2 binding cell budding, cytokinesis, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion, exocytosis lipid binding cell cortex, site of polarized growth, cytoplasm 21337 chr10_2 - - 405 E 5 YOR261C RPN8 - Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p proteolysis involved in cellular protein catabolic process molecular_function cytoplasm 22517 chr15_3 - - 405 E 6 YOR262W - - Protein of unknown function required for establishment of sister chromatid cohesion; contains an ATP/GTP binding site motif; similar to YLR243W and is highly conserved across species and homologous to human gene GPN2/ATPBD1B mitotic cell cycle, organelle fission, chromosome segregation molecular_function cytoplasm 22518 chr15_3 - - 405 E 7 YJL086C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 biological_process molecular_function cellular_component 21338 chr10_2 - - 405 E 8 YOR281C PLP2 - Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP protein folding, transcription from RNA polymerase II promoter, response to chemical stimulus cytoskeletal protein binding cytoplasm 22537 chr15_3 - - 405 E 9 YOR282W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C biological_process molecular_function cellular_component 22538 chr15_3 - - 405 E 10 YOR287C RRP36 - Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 22543 chr15_3 - - 405 E 11 YJL173C RFA3 RPA3 Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination meiotic cell cycle, DNA repair, telomere organization, DNA replication, response to DNA damage stimulus, protein modification by small protein conjugation or removal, DNA recombination DNA binding nucleus, chromosome 21252 chr10_1 - - 405 E 12 YJL194W CDC6 - Essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p mitotic cell cycle, DNA replication, regulation of DNA metabolic process GTPase activity, DNA binding, ATPase activity nucleus 21230 chr10_1 - - 405 F 1 YJL203W PRP21 SPP91 Subunit of the SF3a splicing factor complex, required for spliceosome assembly RNA splicing, mRNA processing RNA binding nucleus 21222 chr10_1 - - 405 F 2 YJL195C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 biological_process molecular_function cellular_component 21231 chr10_1 - - 405 F 3 YJL156C SSY5 - Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p protein maturation, response to chemical stimulus peptidase activity membrane, plasma membrane 21269 chr10_1 - - 405 F 4 YJL202C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex biological_process molecular_function cellular_component 21223 chr10_1 - - 405 F 5 YLR336C SGD1 - Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia ribosomal small subunit biogenesis, response to osmotic stress molecular_function nucleus, nucleolus 25245 chr12_5 - - 405 F 6 YLR339C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 biological_process molecular_function cellular_component 25248 chr12_5 - - 405 F 7 YLR340W RPP0 P0|L10E|A0|RPL10E Conserved ribosomal protein P0 of the ribosomal stalk, which is involved in interaction between translational elongation factors and the ribosome; similar to rat P0, human P0, and E. coli L10e; phosphorylated on serine 302 cytoplasmic translation, organelle assembly, translational elongation, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, rRNA binding, structural molecule activity ribosome, cytoplasm 25249 chr12_5 - - 405 F 8 YLR355C ILV5 - Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids mitochondrion organization, cellular amino acid metabolic process DNA binding, oxidoreductase activity mitochondrion, cytoplasm 25264 chr12_5 - - 405 F 9 YLR359W ADE13 BRA8|BRA1 Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency nucleobase-containing small molecule metabolic process lyase activity cellular_component 25268 chr12_5 - - 405 F 10 YLR378C SEC61 - Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER protein targeting, transmembrane transport, proteolysis involved in cellular protein catabolic process protein transporter activity, transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 25287 chr12_5 - - 405 F 11 YLR379W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C biological_process molecular_function cellular_component 25288 chr12_5 - - 405 F 12 YLR397C AFG2 DRG1 ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; may be involved in degradation of aberrant mRNAs ribosomal large subunit biogenesis, response to chemical stimulus ATPase activity other 25306 chr12_5 - - 405 G 1 YLR409C UTP21 - Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 25318 chr12_5 - - 405 G 2 YLR229C CDC42 - Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins vacuole organization, mitotic cell cycle, regulation of transport, response to chemical stimulus, conjugation, cell budding, cell morphogenesis, invasive growth in response to glucose limitation, regulation of organelle organization, membrane fusion, exocytosis, pseudohyphal growth, regulation of cell cycle, signaling GTPase activity, signal transducer activity plasma membrane, cellular bud, membrane, site of polarized growth 25138 chr12_4 - - 405 G 3 YLR230W - - Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity biological_process molecular_function cellular_component 25139 chr12_4 - - 405 G 4 YLR243W - - Putative protein of unknown function; YLR243W is an essential gene biological_process other cellular_component 25152 chr12_4 - - 405 G 5 YLR249W YEF3 EF-3|eEF3|TEF3 Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP translational elongation RNA binding, translation factor activity, nucleic acid binding, ATPase activity ribosome, cytoplasm 25158 chr12_4 - - 405 G 6 YLR259C HSP60 MNA2|MIF4|CPN60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated protein targeting, mitochondrion organization, transmembrane transport, protein maturation, protein complex biogenesis, protein folding DNA binding, unfolded protein binding, ATPase activity mitochondrion, cytoplasm 25168 chr12_4 - - 405 G 7 YLR272C YCS4 LOC7 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus mitotic cell cycle, meiotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, nucleolus, chromosome 25181 chr12_4 - - 405 G 8 YLR274W MCM5 CDC46|BOB1 Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase mitotic cell cycle, DNA repair, chromatin organization, DNA replication, response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process DNA binding nucleus, chromosome, cytoplasm 25183 chr12_4 - - 405 G 9 YLR275W SMD2 Sm D2 Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 RNA splicing, mRNA processing molecular_function nucleus 25184 chr12_4 - - 405 G 10 YLR276C DBP9 - ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 60S ribosomal subunit ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis helicase activity, ATPase activity nucleus, nucleolus 25185 chr12_4 - - 405 G 11 YLR277C YSH1 BRR5 Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs snoRNA processing, RNA splicing, mRNA processing, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter RNA binding, nuclease activity nucleus 25186 chr12_4 - - 405 G 12 YLR291C GCD7 - Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression translational initiation, regulation of translation RNA binding, translation factor activity, nucleic acid binding, enzyme regulator activity mitochondrion, cytoplasm 25201 chr12_4 - - 405 H 1 YLR298C YHC1 U1C|U1-C Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 25208 chr12_4 - - 405 H 2 YLR305C STT4 - Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization lipid metabolic process kinase activity membrane, mitochondrion, plasma membrane, cytoplasm 25213 chr12_4 - - 405 H 3 YLR310C CDC25 CDC25'|CTN1 Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 mitotic cell cycle, signaling, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 25218 chr12_4 - - 405 H 4 YLR314C CDC3 - Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells cytoskeleton organization, cytokinesis, protein complex biogenesis lipid binding, structural molecule activity cell cortex, cytoskeleton, cell wall, cellular bud, membrane, site of polarized growth, cytoplasm 25223 chr12_4 - - 405 H 6 YLR316C TAD3 - Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs tRNA processing, RNA modification hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cytoplasm 25225 chr12_4 - - 405 H 7 YLR317W - - Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C biological_process molecular_function cellular_component 25226 chr12_4 - - 405 H 8 YLR321C SFH1 - Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog mitotic cell cycle, DNA repair, chromatin organization, DNA replication, chromosome segregation, response to DNA damage stimulus, transcription elongation, DNA-dependent, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle ATPase activity nucleus 25230 chr12_4 - - 405 H 9 YLR323C CWC24 - Essential protein, component of a complex containing Cef1p; has similarity to S. pombe Cwf24p snoRNA processing, RNA splicing molecular_function nucleus 25232 chr12_4 - - 405 H 10 YLR293C GSP1 CST17|CNR1 Ran GTPase, GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; yeast Gsp2p homolog rRNA processing, nuclear transport, nucleus organization GTPase activity nucleus, membrane, cytoplasm 25203 chr12_4 - - 405 H 11 YDR160W SSY1 SHR10 Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes response to chemical stimulus molecular_function membrane, plasma membrane 23518 chr4_5 - - 405 H 12 YDR164C SEC1 - Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function regulation of organelle organization, regulation of transport, organelle fusion, vesicle organization, membrane fusion, exocytosis other cellular bud, membrane, site of polarized growth, plasma membrane 23522 chr4_5 - - 406 A 1 YDR166C SEC5 - Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis cell budding, cytokinesis, organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, exocytosis molecular_function cell cortex, cellular bud, site of polarized growth, cytoplasm 23524 chr4_5 - - 406 A 2 YDR167W TAF10 TafII25|TAF25|TAF23 Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification peptidyl-amino acid modification, protein acylation, chromatin organization, transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, histone modification nucleic acid binding transcription factor activity, structural molecule activity nucleus 23525 chr4_5 - - 406 A 3 YDR168W CDC37 SMO1 Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding cytoskeleton organization, signaling, response to osmotic stress, regulation of cell cycle unfolded protein binding cytoplasm 23526 chr4_5 - - 406 A 4 YDR170C SEC7 - Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles vacuole organization, organelle assembly, Golgi vesicle transport, response to starvation enzyme regulator activity cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 23528 chr4_5 - - 406 A 5 YDR172W SUP35 eRF3|[PSI(+)]|[PSI]|SUP36|SUP2|SUF12|SAL3|PNM2|GST1 Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype RNA catabolic process RNA binding, translation factor activity, nucleic acid binding cytoplasm 23530 chr4_5 - - 406 A 7 YDR177W UBC1 - Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response membrane invagination, protein modification by small protein conjugation or removal, vesicle organization, proteolysis involved in cellular protein catabolic process, endocytosis ligase activity other 23535 chr4_5 - - 406 A 8 YDR180W SCC2 - Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin mitotic cell cycle, DNA repair, organelle fission, chromosome segregation, response to DNA damage stimulus molecular_function nucleus, chromosome 23539 chr4_5 - - 406 A 9 YDR182W CDC1 ESP2|DSR1|DSC1 Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution DNA repair, cytoskeleton organization, response to DNA damage stimulus, cellular ion homeostasis molecular_function membrane, endoplasmic reticulum, cytoplasm 23541 chr4_5 - - 406 A 10 YDR187C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W biological_process molecular_function cellular_component 23546 chr4_5 - - 406 A 11 YDR188W CCT6 TCP20|TCP6|HTR3 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif protein folding unfolded protein binding cytoplasm 23547 chr4_5 - - 406 A 12 YDR189W SLY1 - Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex regulation of organelle organization, regulation of transport, organelle fusion, vesicle organization, protein complex biogenesis, Golgi vesicle transport, membrane fusion other endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 23548 chr4_5 - - 406 B 1 YDR190C RVB1 TIP49|TIH1|TIP49A Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; also referred to as pontin; member of the RUVB-like protein family chromatin organization, transcription from RNA polymerase II promoter helicase activity, ATPase activity nucleus, chromosome 23549 chr4_5 - - 406 B 2 YDR196C CAB5 - Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies cofactor metabolic process, nucleobase-containing small molecule metabolic process kinase activity nucleus, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 23555 chr4_5 - - 406 B 3 YDR201W SPC19 - Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body mitotic cell cycle, cytoskeleton organization, regulation of organelle organization cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 23560 chr4_5 - - 406 B 4 YDR208W MSS4 - Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation lipid metabolic process, sporulation kinase activity nucleus, membrane, plasma membrane 23567 chr4_5 - - 406 B 5 YDR211W GCD6 - Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression translational initiation, regulation of translation RNA binding, translation factor activity, nucleic acid binding, enzyme regulator activity cytoplasm 23570 chr4_5 - - 406 B 6 YDR212W TCP1 CCT1 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein protein folding unfolded protein binding membrane fraction, cytoplasm 23571 chr4_5 - - 406 B 7 YDR224C HTB1 SPT12 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation response to DNA damage stimulus, DNA repair, chromatin organization, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome 23583 chr4_5 - - 406 B 8 YDR228C PCF11 - mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping transcription termination, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter enzyme binding, RNA binding, mRNA binding nucleus 23587 chr4_5 - - 406 B 9 YDR232W HEM1 OLE3|CYD1 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p cofactor metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm 23591 chr4_5 - - 406 B 10 YDR235W PRP42 SNU65|MUD16 U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats RNA splicing, mRNA processing RNA binding nucleus 23594 chr4_5 - - 406 B 11 YDR236C FMN1 - Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enzymes; localizes to microsomes and to the mitochondrial inner membrane cofactor metabolic process, nucleobase-containing small molecule metabolic process kinase activity membrane fraction, mitochondrion, membrane, mitochondrial envelope, cytoplasm 23595 chr4_5 - - 406 B 12 YDR238C SEC26 - Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 23597 chr4_5 - - 406 C 1 YDR240C SNU56 MUD10 Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 23599 chr4_5 - - 406 C 2 YGL201C MCM6 - Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity, helicase activity nucleus, chromosome, cytoplasm 24568 chr7_3 - - 406 C 3 YGL207W SPT16 SSF1|CDC68 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases chromatin organization, DNA replication, protein complex biogenesis, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter histone binding nucleus, chromosome 24573 chr7_3 - - 406 C 4 YGL225W VRG4 VIG4|LDB3|GOG5|VAN2 Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi carbohydrate metabolic process, protein glycosylation, lipid metabolic process, carbohydrate transport transmembrane transporter activity mitochondrion, membrane, Golgi apparatus, cytoplasm 24591 chr7_3 met- did not mate 406 C 5 YGL233W SEC15 - Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which mediates polarized targeting of vesicles to active sites of exocytosis; Sec15p associates with Sec4p and vesicles cell budding, cytokinesis, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion, exocytosis enzyme binding cell cortex, site of polarized growth, cytoplasm 24600 chr7_3 - - 406 C 6 YGL238W CSE1 KAP109 Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation protein targeting, nuclear transport protein transporter activity nucleus, membrane, endomembrane system 24605 chr7_3 - - 406 C 7 YGL245W GUS1 GluRS|GSN1 Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 24612 chr7_3 - - 406 C 8 YGL247W BRR6 - Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism protein targeting, lipid metabolic process, nucleobase-containing compound transport, nuclear transport molecular_function nucleus, endomembrane system 24614 chr7_3 - - 406 C 9 YGR002C SWC4 GOD1|EAF2 Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex peptidyl-amino acid modification, mitotic cell cycle, DNA repair, protein acylation, organelle fission, chromatin organization, histone modification, chromosome segregation, response to DNA damage stimulus DNA binding nucleus, chromosome 24632 chr7_3 - - 406 C 10 YGR005C TFG2 - TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 24635 chr7_3 - - 406 C 11 YGR009C SEC9 HSS7 t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog membrane fusion, Golgi vesicle transport, organelle fusion, vesicle organization other membrane, plasma membrane 24639 chr7_3 - - 406 C 12 YGR013W SNU71 - Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart RNA splicing, mRNA processing RNA binding nucleus 24643 chr7_3 - - 406 D 1 YPL007C TFC8 tau 60 One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 transcription from RNA polymerase III promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus 22821 chr16_3 - - 406 D 2 YPL010W RET3 - Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 22818 chr16_3 - - 406 D 3 YPL011C TAF3 TafII47|TAF47 TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 22817 chr16_3 - - 406 D 4 YPL012W RRP12 - Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, ribosome, cytoplasm 22816 chr16_3 - - 406 D 5 YPL016W SWI1 [SWI(+)]|[SWI+]|LPA1|GAM3|ADR6 Subunit of the SWI/SNF chromatin remodeling complex, which regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer chromatin organization, transcription from RNA polymerase II promoter molecular_function nucleus 22812 chr16_3 - - 406 D 6 YPL020C ULP1 NIB1 Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions mitotic cell cycle, protein modification by small protein conjugation or removal peptidase activity nucleus, nucleolus, membrane, endomembrane system 22808 chr16_3 - - 406 D 7 YPL028W ERG10 TSM0115|LPB3 Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis lipid metabolic process transferase activity, transferring acyl groups cytoplasm 22800 chr16_3 - - 406 D 8 YPL043W NOP4 NOP77 Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) rRNA processing RNA binding nucleus, nucleolus 22784 chr16_3 - - 406 D 9 YPL044C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W biological_process molecular_function cellular_component 22785 chr16_3 - - 406 D 10 YPL063W TIM50 - Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 22765 chr16_3 - - 406 D 11 YPL075W GCR1 LPF10 Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p carbohydrate metabolic process, chromatin organization, generation of precursor metabolites and energy, response to chemical stimulus, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, endomembrane system 22753 chr16_3 - - 406 D 12 YPL076W GPI2 GCR4 Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein lipid metabolic process, protein lipidation transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 22752 chr16_3 - - 406 E 1 YPL082C MOT1 BTAF1|LPF4|BUR3 Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity transcription from RNA polymerase I promoter, rRNA processing, protein complex biogenesis, transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity, transcription factor binding nucleus, chromosome, mitochondrion, cytoplasm 22746 chr16_3 - - 406 E 2 YPL083C SEN54 tRNA splicing endonuclease subunit Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p RNA splicing, tRNA processing nuclease activity nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm 22745 chr16_3 - - 406 E 3 YPL085W SEC16 LPF1 COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Golgi vesicle transport, vesicle organization other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm 22743 chr16_3 - - 406 E 4 YPR105C COG4 SGF1|SEC38|COD1 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting, response to starvation, peroxisome organization molecular_function cytoplasm, Golgi apparatus 25521 chr16_5 - - 406 E 5 YPR107C YTH1 - Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation mRNA processing RNA binding nucleus 25523 chr16_5 - - 406 E 6 YPR108W RPN7 - Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits proteolysis involved in cellular protein catabolic process structural molecule activity nucleus 25524 chr16_5 - - 406 E 7 YPR110C RPC40 AC40|RPC5 RNA polymerase subunit AC40, common to RNA polymerase I and III transcription from RNA polymerase I promoter, transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus, nucleolus 25526 chr16_5 - - 406 E 8 YPR112C MRD1 - Essential conserved protein that is part of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains ribosome assembly, rRNA processing, organelle assembly, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 25528 chr16_5 - - 406 E 9 YPR113W PIS1 - Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins lipid metabolic process other membrane fraction, mitochondrion, membrane, mitochondrial envelope, Golgi apparatus, cytoplasm 25529 chr16_5 - - 406 E 10 YPR133C SPN1 IWS1 Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, nucleobase-containing compound transport, nuclear transport protein binding transcription factor activity nucleus 25548 chr16_5 - - 406 E 11 YPR136C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 biological_process molecular_function cellular_component 25551 chr16_5 - - 406 E 12 YPR137W RRP9 - Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein rRNA processing, RNA modification RNA binding nucleus, nucleolus 25552 chr16_5 - - 406 F 1 YPR142C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing biological_process molecular_function cellular_component 25557 chr16_5 - - 406 F 2 YPR143W RRP15 - Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs rRNA processing, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 25558 chr16_5 - - 406 F 3 YPR144C NOC4 UTP19 Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus, cytoplasm 25559 chr16_5 - - 406 F 4 YPR161C SGV1 BUR1 Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins protein phosphorylation, protein alkylation, chromatin organization, regulation of organelle organization, histone modification, regulation of protein modification process, transcription from RNA polymerase II promoter kinase activity nucleus 25576 chr16_5 - - 406 F 5 YPR162C ORC4 - Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing DNA replication DNA binding nucleus, chromosome 25577 chr16_5 - - 406 F 6 YPR165W RHO1 - GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) vacuole organization, cytoskeleton organization, regulation of transport, cell wall organization or biogenesis, carbohydrate metabolic process, cell budding, regulation of organelle organization, membrane fusion, membrane invagination, signaling, endocytosis GTPase activity, signal transducer activity peroxisome, membrane fraction, mitochondrion, plasma membrane, Golgi apparatus, cellular bud, membrane, site of polarized growth, mitochondrial envelope, cytoplasm 25580 chr16_5 - - 406 F 7 YPR168W NUT2 MED10 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription transcription from RNA polymerase II promoter molecular_function nucleus 25583 chr16_5 - - 406 F 8 YPR169W JIP5 - Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 25584 chr16_5 - - 406 F 9 YPR175W DPB2 - Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate DNA repair, DNA replication, response to DNA damage stimulus nucleotidyltransferase activity, DNA binding nucleus, chromosome, cytoplasm 25590 chr16_5 - - 406 F 10 YPR176C BET2 - Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Golgi vesicle transport, protein targeting transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 25591 chr16_5 - - 406 F 11 YPR177C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex biological_process molecular_function cellular_component 25592 chr16_5 - - 406 F 12 YPR178W PRP4 RNA4 Splicing factor, component of the U4/U6-U5 snRNP complex RNA splicing, mRNA processing molecular_function nucleus 25593 chr16_5 - - 406 G 1 YPR180W AOS1 RHC31 Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein modification by small protein conjugation or removal other nucleus 25595 chr16_5 - - 406 G 2 YPR181C SEC23 - GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p regulation of organelle organization, regulation of transport, vesicle organization, Golgi vesicle transport, nucleobase-containing small molecule metabolic process enzyme regulator activity endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm 25596 chr16_5 - - 406 G 3 YPR182W SMX3 Sm F|SmF Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F RNA splicing, mRNA processing molecular_function nucleus 25597 chr16_5 - - 406 G 4 YPR183W DPM1 SED3 Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation carbohydrate metabolic process, protein glycosylation, lipid metabolic process, protein lipidation transferase activity, transferring glycosyl groups endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 25598 chr16_5 - - 406 G 5 YPR186C PZF1 TFC2 Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA transcription from RNA polymerase III promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus 25601 chr16_5 - - 406 G 6 YPR187W RPO26 ABC23|RPB6 RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit transcription from RNA polymerase I promoter, transcription from RNA polymerase III promoter, transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus, nucleolus 25602 chr16_5 - - 406 G 7 YPR190C RPC82 RPC80|C82|RPC3 RNA polymerase III subunit C82 transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus 25605 chr16_5 - - 406 G 8 YCR093W CDC39 SMD6|ROS1|NOT1 Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to chemical stimulus, RNA catabolic process, conjugation, pseudohyphal growth, transcription elongation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter, regulation of cell cycle molecular_function nucleus, cytoplasm 25812 Chr00_2 - - 406 G 9 YER048W-A ISD11 - Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis cofactor metabolic process enzyme regulator activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 25839 Chr00_2 - - 406 G 10 YFR037C RSC8 SWH3 Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters response to DNA damage stimulus, mitotic cell cycle, DNA repair, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity nucleus 25851 Chr00_2 - - 406 G 11 YFR042W KEG1 - Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability carbohydrate metabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 25856 Chr00_2 - - 406 G 12 YFR050C PRE4 - Beta 7 subunit of the 20S proteasome proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 25864 Chr00_2 - - 406 H 1 YFR051C RET2 - Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Golgi vesicle transport, organelle inheritance molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 25865 Chr00_2 - - 406 H 2 YFR052W RPN12 NIN1 Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p proteolysis involved in cellular protein catabolic process molecular_function cytoplasm 25866 Chr00_2 - - 406 H 3 YGR245C SDA1 - Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis mitotic cell cycle, cytoskeleton organization, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport, regulation of cell cycle molecular_function nucleus 25898 Chr00_2 - - 406 H 4 YGR246C BRF1 TFIIIB70|TDS4|PCF4 TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB transcription initiation, DNA-dependent, transcription from RNA polymerase III promoter, transposition enzyme binding, protein binding transcription factor activity, DNA binding, transcription factor binding nucleus 25899 Chr00_2 - - 406 H 6 YGR251W NOP19 DHI1 Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 25904 Chr00_2 - - 406 H 7 YBL004W UTP20 - Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus, cytoplasm 23028 chr2_1 - - 406 H 8 YBL014C RRN6 - Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription from RNA polymerase I promoter, transcription initiation, DNA-dependent protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus, nucleolus 23040 chr2_1 - - 406 H 9 YBL018C POP8 - Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs snoRNA processing, tRNA processing, rRNA processing, mRNA processing nuclease activity nucleus, nucleolus 23044 chr2_1 - - 406 H 10 YBL020W RFT1 - Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein carbohydrate metabolic process, protein glycosylation, lipid transport molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm 23046 chr2_1 - - 406 H 11 YBL023C MCM2 - Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination DNA binding nucleus, chromosome, cytoplasm 23049 chr2_1 - - 406 H 12 YBL026W LSM2 SNP3|SMX5 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA RNA catabolic process, RNA splicing, mRNA processing RNA binding nucleus, nucleolus, cytoplasm 23052 chr2_1 - - 407 A 1 YBL030C PET9 OP1|ANC2|AAC2 Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation transmembrane transport, nucleobase-containing compound transport, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 23056 chr2_1 - - 407 A 2 YBL034C STU1 - Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles cytoskeleton organization structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 23060 chr2_1 met- did not mate 407 A 3 YBL035C POL12 - B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation telomere organization, DNA replication nucleotidyltransferase activity nucleus, endomembrane system, chromosome 23061 chr2_1 - - 407 A 4 YBL040C ERD2 - HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Golgi vesicle transport other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 23066 chr2_1 - - 407 A 5 YBL041W PRE7 PRS3 Beta 6 subunit of the 20S proteasome proteolysis involved in cellular protein catabolic process molecular_function nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 23067 chr2_1 - - 407 A 6 YBL050W SEC17 RNS3 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP vacuole organization, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion enzyme regulator activity membrane fraction, membrane, cytoplasm 23076 chr2_1 - - 407 A 8 YBL073W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C biological_process molecular_function cellular_component 23099 chr2_1 - - 407 A 9 YBL074C AAR2 - Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron RNA splicing, mRNA processing molecular_function nucleus 23100 chr2_1 - - 407 A 10 YBL076C ILS1 isoleucyl-tRNA synthetase Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A cytoplasmic translation, tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity cytoplasm 23102 chr2_1 - - 407 A 11 YBL077W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C biological_process molecular_function cellular_component 23103 chr2_1 - - 407 A 12 YBL084C CDC27 SNB1|APC3 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus 23110 chr2_1 - - 407 B 1 YBL092W RPL32 L32 Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein; overexpression disrupts telomeric silencing cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 23118 chr2_1 - - 407 B 2 YGL001C ERG26 - C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 24369 chr7_1 - - 407 B 3 YGL008C PMA1 KTI10 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] transmembrane transport, ion transport transmembrane transporter activity, ATPase activity membrane fraction, mitochondrion, membrane, cytoplasm, plasma membrane 24376 chr7_1 - - 407 B 4 YGL011C SCL1 PRC2 Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria proteolysis involved in cellular protein catabolic process molecular_function nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 24379 chr7_1 - - 407 B 5 YGL022W STT3 - Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 24390 chr7_1 - - 407 B 6 YGL030W RPL30 rp73|YL38|L32|L30 Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript cytoplasmic translation, RNA splicing, regulation of translation, rRNA processing, mRNA processing RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 24398 chr7_1 - - 407 B 7 YGL040C HEM2 OLE4|SLU1 Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus cofactor metabolic process lyase activity nucleus, cytoplasm 24408 chr7_1 - - 407 B 8 YGL044C RNA15 - Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA processing RNA binding, mRNA binding nucleus 24412 chr7_1 - - 407 B 9 YGL048C RPT6 SUG1|SCB68|CRL3|CIM3 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle DNA repair, chromatin organization, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, response to DNA damage stimulus, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity nucleus, cytoplasm 24416 chr7_1 - - 407 B 10 YGL055W OLE1 MDM2 Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 24422 chr7_1 - - 407 B 11 YGL061C DUO1 - Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic cell cycle, cytoskeleton organization, regulation of organelle organization cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 24428 chr7_1 - - 407 B 12 YGL065C ALG2 - Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol carbohydrate metabolic process, protein glycosylation, lipid metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 24432 chr7_1 - - 407 C 1 YGL069C - SRF3 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component 24436 chr7_1 - - 407 C 2 YGL073W HSF1 MAS3|EXA3 Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated response to heat, cytoskeleton organization, transcription from RNA polymerase II promoter, signaling nucleic acid binding transcription factor activity, DNA binding nucleus, mitochondrion, cytoplasm 24440 chr7_1 - - 407 C 3 YGL074C - - Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor biological_process molecular_function cellular_component 24441 chr7_1 - - 407 C 4 YGL075C MPS2 MMC1 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p mitotic cell cycle, organelle fission, cytoskeleton organization structural molecule activity nucleus, cytoskeleton, microtubule organizing center, endomembrane system, cytoplasm 24442 chr7_1 - - 407 C 5 YGL091C NBP35 - Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases cofactor metabolic process, tRNA processing, RNA modification ion binding, ATPase activity nucleus, cytoplasm 24458 chr7_1 - - 407 C 6 YGL092W NUP145 RAT10 Essential nucleoporin, subunit of the Nup84p subcomplex; catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p; the Nup84 subcomplex has a role in transcription elongation protein targeting, nucleobase-containing compound transport, nuclear transport, nucleus organization RNA binding, structural molecule activity nucleus, endomembrane system, membrane 24459 chr7_1 - - 407 C 7 YNL149C PGA2 - Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect other molecular_function nucleus, endomembrane system, endoplasmic reticulum, cytoplasm 22063 chr14_2 - - 407 C 8 YNL150W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking biological_process molecular_function cellular_component 22062 chr14_2 - - 407 C 9 YNL151C RPC31 C31|RPC8|ACP2 RNA polymerase III subunit C31 transcription initiation, DNA-dependent, transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus 22061 chr14_2 - - 407 C 10 YNL158W PGA1 - Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes lipid metabolic process, protein lipidation transferase activity, transferring glycosyl groups nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 22054 chr14_2 - - 407 C 11 YNL161W CBK1 - Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization protein phosphorylation, cell budding, cytoskeleton organization, cytokinesis, cell wall organization or biogenesis kinase activity nucleus, cell cortex, cellular bud, site of polarized growth, cytoplasm 22051 chr14_2 - - 407 C 12 YNL163C RIA1 EFL1 Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p) rRNA processing, ribosomal large subunit biogenesis GTPase activity cytoplasm 22049 chr14_2 - - 407 D 1 YNL178W RPS3 rp13|YS3|S3|SUF14 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; has similarity to E. coli S3 and rat S3 ribosomal proteins cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural constituent of ribosome, lyase activity, structural molecule activity ribosome, cytoplasm 22034 chr14_2 - - 407 D 2 YNL181W - - Putative oxidoreductase; required for cell viability biological_process oxidoreductase activity nucleus, endomembrane system, endoplasmic reticulum, cytoplasm 22031 chr14_2 - - 407 D 3 YNL182C IPI3 - Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; highly conserved and contains WD40 motifs; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus 22030 chr14_2 - - 407 D 4 YNL189W SRP1 SCM1|KAP60 Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation protein targeting, nuclear transport protein transporter activity nucleus, membrane, cytoplasm 22023 chr14_2 - - 407 D 5 YNL207W RIO2 - Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p rRNA processing, ribosomal small subunit biogenesis kinase activity nucleus, cytoplasm 22005 chr14_2 - - 407 D 6 YNL216W RAP1 TUF1|TBA1|GRF1 DNA-binding protein involved in either activation or repression of transcription, depending on binding site context; also binds telomere sequences and plays a role in telomeric position effect (silencing) and telomere structure telomere organization, DNA replication, response to DNA damage stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, endomembrane system, chromosome 21996 chr14_2 - - 407 D 7 YNL221C POP1 - Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P snoRNA processing, tRNA processing, rRNA processing, mRNA processing nuclease activity nucleus, nucleolus 21991 chr14_2 - - 407 D 8 YNL222W SSU72 - Transcription/RNA-processing factor that associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 of the RNA polymerase II C-terminal domain; affects start site selection in vivo rRNA processing, transcription initiation, DNA-dependent, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter phosphatase activity, RNA binding nucleus 21990 chr14_2 - - 407 D 9 YNL232W CSL4 SKI4 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) RNA catabolic process, snoRNA processing, rRNA processing molecular_function nucleus, cytoplasm 21980 chr14_2 - - 407 D 10 YNL240C NAR1 - Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf cofactor metabolic process oxidoreductase activity membrane, cytoplasm 21972 chr14_2 - - 407 D 11 YKL192C ACP1 - Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p lipid metabolic process, cofactor metabolic process other mitochondrion, cytoplasm 25042 chr11_3 - - 407 D 12 YKL193C SDS22 EGP1 Conserved nuclear regulatory subunit of Glc7p type 1 protein serine-threonine phosphatase (PP1), functions positively with Glc7p to promote dephosphorylation of nuclear substrates required for chromosome transmission during mitosis chromosome segregation enzyme regulator activity nucleus, cytoplasm 25043 chr11_3 - - 407 E 1 YKL196C YKT6 - Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus vacuole organization, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion transferase activity, transferring acyl groups vacuole, mitochondrion, membrane, Golgi apparatus, cytoplasm 25046 chr11_3 - - 407 E 2 YKL203C TOR2 DRR2 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis mitotic cell cycle, cytoskeleton organization, regulation of transport, membrane invagination, signaling, regulation of cell cycle, endocytosis kinase activity membrane fraction, vacuole, mitochondrion, membrane, cytoplasm, plasma membrane 25053 chr11_3 - - 407 E 3 YKL210W UBA1 - Ubiquitin activating enzyme (E1), involved in ubiquitin-mediated protein degradation and essential for viability protein modification by small protein conjugation or removal other nucleus, cytoplasm 25060 chr11_3 - - 407 E 4 YKR002W PAP1 - Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping mRNA processing nucleotidyltransferase activity nucleus 25073 chr11_3 - - 407 E 5 YKR008W RSC4 - Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p mitotic cell cycle, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter histone binding, ATPase activity nucleus 25079 chr11_3 - - 407 E 6 YKR022C NTR2 - Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly RNA splicing, mRNA processing molecular_function nucleus, endoplasmic reticulum, cytoplasm 25093 chr11_3 - - 407 E 7 YKR025W RPC37 C37 RNA polymerase III subunit C37 transcription from RNA polymerase III promoter nucleotidyltransferase activity nucleus 25096 chr11_3 - - 407 E 8 YKR037C SPC34 - Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body mitotic cell cycle, cytoskeleton organization, regulation of organelle organization cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 25108 chr11_3 - - 407 E 9 YKR062W TFA2 - TFIIE small subunit, involved in RNA polymerase II transcription initiation transcription from RNA polymerase II promoter enzyme binding, DNA binding, transcription factor binding nucleus 25133 chr11_3 - - 407 E 10 YKR063C LAS1 - Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability rRNA processing, ribosomal large subunit biogenesis molecular_function nucleus, mitochondrion, cytoplasm 25134 chr11_3 - - 407 E 11 YKL209C STE6 - Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells signaling transmembrane transporter activity, ATPase activity membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 25059 chr11_3 - - 407 E 12 YDR243C PRP28 - RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site RNA splicing, mRNA processing helicase activity, ATPase activity nucleus 23602 chr4_6 - - 407 F 1 YDR246W TRS23 - One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Golgi vesicle transport enzyme regulator activity cytoplasm, Golgi apparatus 23605 chr4_6 - - 407 F 2 YDR280W RRP45 - Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9) RNA catabolic process, snoRNA processing, rRNA processing molecular_function nucleus, nucleolus, cytoplasm 23639 chr4_6 - - 407 F 3 YDR288W NSE3 - Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair response to DNA damage stimulus, DNA repair DNA binding nucleus, chromosome, cytoplasm 23647 chr4_6 - - 407 F 4 YDR292C SRP101 - Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p protein targeting other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 23651 chr4_6 - - 407 F 5 YDR299W BFR2 - Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock rRNA processing molecular_function nucleus, nucleolus 23658 chr4_6 - - 407 F 6 YDR301W CFT1 YHH1 RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF transcription termination, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter RNA binding nucleus, mitochondrion, cytoplasm 23660 chr4_6 - - 407 F 7 YDR302W GPI11 - ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog lipid metabolic process, protein lipidation other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 23661 chr4_6 - - 407 F 8 YDR303C RSC3 - Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p mitotic cell cycle, chromatin organization, DNA replication, regulation of DNA metabolic process, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter, regulation of cell cycle DNA binding, ATPase activity nucleus 23662 chr4_6 - - 407 F 9 YDR308C SRB7 MED21 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 23667 chr4_6 - - 407 F 10 YDR311W TFB1 - Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators transcription from RNA polymerase I promoter, DNA repair, protein phosphorylation, response to DNA damage stimulus, transcription from RNA polymerase II promoter protein binding transcription factor activity, lipid binding nucleus 23670 chr4_6 - - 407 F 11 YDR324C UTP4 - Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript transcription from RNA polymerase I promoter, rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 23683 chr4_6 - - 407 F 12 YDR325W YCG1 YCS5|TIE1 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus mitotic cell cycle, meiotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, chromosome 23684 chr4_6 - - 407 G 1 YDR328C SKP1 CBF3D|MGO1 Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase mitotic cell cycle, cytoskeleton organization, organelle assembly, cytokinesis, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, cellular ion homeostasis, protein modification by small protein conjugation or removal, regulation of cell cycle ligase activity, DNA binding nucleus, chromosome, cytoplasm 23687 chr4_6 - - 407 G 2 YDR331W GPI8 - ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog lipid metabolic process, protein lipidation other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 23690 chr4_6 - - 407 G 3 YIL004C BET1 SLY12 Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion other endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, cytoplasm 21396 chr9_1 - - 407 G 4 YIL021W RPB3 B44 RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit transcription termination, DNA-dependent, transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus 21414 chr9_1 - - 407 G 5 YIL022W TIM44 MPI1|MIM44|ISP45 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) protein targeting, mitochondrion organization, transmembrane transport protein binding, bridging membrane, mitochondrial envelope, mitochondrion, cytoplasm 21415 chr9_1 - - 407 G 6 YIL026C IRR1 SCC3 Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability mitotic cell cycle, organelle fission, conjugation, cell wall organization or biogenesis, chromosome segregation other nucleus, chromosome 21419 chr9_1 - - 407 G 7 YIL046W MET30 ZRG11 F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus mitotic cell cycle, DNA replication, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, protein modification by small protein conjugation or removal, regulation of DNA metabolic process, transcription from RNA polymerase II promoter small conjugating protein binding nucleus 21439 chr9_1 - - 407 G 8 YIL048W NEO1 - Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus vacuole organization, Golgi vesicle transport, membrane invagination, endocytosis transmembrane transporter activity, ATPase activity endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 21441 chr9_1 - - 407 G 9 YIL063C YRB2 - Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential protein targeting, ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, membrane, endomembrane system 21456 chr9_1 - - 407 G 10 YIL068C SEC6 - Essential 88kDa subunit of the exocyst complex, which mediates polarized targeting of secretory vesicles to active sites of exocytosis; dimeric form of Sec6p interacts with Sec9p in vitro and inhibits t-SNARE assembly cell budding, cytokinesis, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion, exocytosis other cell cortex, site of polarized growth, cytoplasm 21461 chr9_1 - - 407 G 11 YIL078W THS1 threonyl-tRNA synthetase Threonyl-tRNA synthetase, essential cytoplasmic protein cytoplasmic translation, tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 21471 chr9_1 - - 407 G 12 YFL008W SMC1 CHL10 Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure mitotic cell cycle, DNA repair, organelle fission, chromosome segregation, response to DNA damage stimulus DNA binding, ATPase activity nucleus, chromosome 25624 chr6_1 - - 407 H 1 YFL009W CDC4 - F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p mitotic cell cycle, meiotic cell cycle, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process small conjugating protein binding, ligase activity nucleus 25625 chr6_1 - - 407 H 2 YFL017C GNA1 PAT1 Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA carbohydrate metabolic process transferase activity, transferring acyl groups nucleus, cytoplasm 25635 chr6_1 - - 407 H 3 YFL018W-A - - - - - - 25637 chr6_1 - - 407 H 4 YFL022C FRS2 cytoplasmic phenylalanyl-tRNA synthetase alpha subunit Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity cytoplasm 25641 chr6_1 - - 407 H 6 YFL024C EPL1 - Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); homologous to Drosophila Enhancer of Polycomb peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, histone modification, response to DNA damage stimulus, transcription from RNA polymerase II promoter transferase activity, transferring acyl groups nucleus 25643 chr6_1 - - 407 H 7 YFL035C - - - - - - 25654 chr6_1 - - 407 H 8 YFL035C-A - - - - - - 25655 chr6_1 - - 407 H 9 YFL037W TUB2 SHE8|ARM10 Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules mitotic cell cycle, meiotic cell cycle, organelle fission, chromosome segregation, conjugation structural molecule activity nucleus, cytoskeleton, microtubule organizing center, cytoplasm 25658 chr6_1 - - 407 H 10 YFL038C YPT1 YP2 Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) protein targeting, vesicle organization, protein complex biogenesis, Golgi vesicle transport, endosome transport, response to starvation GTPase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 25659 chr6_1 - - 407 H 11 YFL045C SEC53 ALG4 Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen protein targeting isomerase activity cytoplasm 25666 chr6_1 - - 407 H 12 YFR003C YPI1 - Inhibitor of the type I protein phosphatase Glc7p, which is involved in regulation of a variety of metabolic processes; overproduction causes decreased cellular content of glycogen carbohydrate metabolic process, protein dephosphorylation, generation of precursor metabolites and energy enzyme regulator activity nucleus 25682 chr6_1 - - 408 A 1 YFR004W RPN11 MPR1 Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes organelle fission, mitochondrion organization, proteolysis involved in cellular protein catabolic process, peroxisome organization, protein modification by small protein conjugation or removal peptidase activity nucleus, mitochondrion, cytoplasm 25683 chr6_1 - - 408 A 2 YFR027W ECO1 CTF7 Acetyltransferase required for establishment of sister chromatid cohesion; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome peptidyl-amino acid modification, mitotic cell cycle, DNA repair, telomere organization, protein acylation, organelle fission, DNA replication, regulation of organelle organization, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle transferase activity, transferring acyl groups nucleus, chromosome 25707 chr6_1 - - 408 A 3 YGR253C PUP2 DOA5 Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta proteolysis involved in cellular protein catabolic process molecular_function nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 25906 Chr00_3 lys- did not mate 408 A 4 YGR264C MES1 MetRS|methionyl-tRNA synthetase|MESI Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity cytoplasm 25916 Chr00_3 - - 408 A 5 YGR265W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase biological_process molecular_function cellular_component 25917 Chr00_3 - - 408 A 6 YGR267C FOL2 - GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway cofactor metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cytoplasm 25919 Chr00_3 - - 408 A 7 YGR274C TAF1 KAT4|TafII145|TafII130|TAF145|TAF130 TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, structural molecule activity, transcription factor binding, transferase activity, transferring acyl groups nucleus 25926 Chr00_3 - - 408 A 9 YGR277C CAB4 - Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved cofactor metabolic process, nucleobase-containing small molecule metabolic process nucleotidyltransferase activity nucleus, cytoplasm 25929 Chr00_3 - - 408 A 10 YGR278W CWC22 - Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein RNA splicing, mRNA processing molecular_function nucleus 25930 Chr00_3 - - 408 A 11 YGR280C PXR1 PinX1|GNO1 Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain telomere organization, snoRNA processing, DNA replication, regulation of organelle organization, regulation of DNA metabolic process enzyme regulator activity nucleus, nucleolus 25932 Chr00_3 - - 408 A 12 YHR005C-A TIM10 MRS11 Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein targeting, mitochondrion organization protein transporter activity, unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 25946 Chr00_3 - - 408 B 1 YIR022W SEC11 - 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum protein targeting, protein maturation peptidase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 25957 Chr00_3 - - 408 B 2 YKR068C BET3 - Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Golgi vesicle transport enzyme regulator activity membrane, endomembrane system, cytoplasm, Golgi apparatus 25984 Chr00_3 - - 408 B 3 YKR071C DRE2 - Conserved component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18p; ortholog of human Ciapin1 cofactor metabolic process other mitochondrial envelope, mitochondrion, cytoplasm 25987 Chr00_3 - - 408 B 4 YKR079C TRZ1 - tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 tRNA processing nuclease activity nucleus, mitochondrion, cytoplasm 25995 Chr00_3 - - 408 B 5 YKR081C RPF2 - Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus 25997 Chr00_3 - - 408 B 6 YKR083C DAD2 HSK1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic cell cycle, cytoskeleton organization, regulation of organelle organization cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, chromosome 25999 Chr00_3 - - 408 B 7 YMR059W SEN15 tRNA splicing endonuclease subunit Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p RNA splicing, tRNA processing nuclease activity nucleus 26193 chr00_6 - - 408 B 8 YMR061W RNA14 - Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA processing RNA binding nucleus, mitochondrion, cytoplasm 26194 chr00_6 - - 408 B 9 YMR076C PDS5 - Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, chromosome segregation, response to DNA damage stimulus structural molecule activity nucleus, chromosome 26210 chr00_6 - - 408 B 10 YMR079W SEC14 PIT1 Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; functionally homologous to mammalian PITPs Golgi vesicle transport, lipid transport, lipid metabolic process, sporulation other cytoplasm, Golgi apparatus 26213 chr00_6 - - 408 B 11 YMR093W UTP15 - Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA transcription from RNA polymerase I promoter, rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus, chromosome 26228 chr00_6 - - 408 B 12 YOL094C RFC4 - Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon response to DNA damage stimulus, DNA repair, DNA replication, chromosome segregation DNA binding, ATPase activity chromosome 26244 chr00_6 - - 408 C 1 YOL097C WRS1 tryptophanyl-tRNA synthetase|HRE342 Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity cytoplasm 26247 chr00_6 - - 408 C 2 YOL102C TPT1 tRNA 2'-phosphotransferase tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide RNA splicing, tRNA processing other nucleus 26252 chr00_6 - - 408 C 3 YOL120C RPL18A rp28A|L18A|RP28A Protein component of the large (60S) ribosomal subunit, identical to Rpl18Bp and has similarity to rat L18 ribosomal protein; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 26270 chr00_6 - - 408 C 4 YOL123W HRP1 NAB5|NAB4 Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences mRNA processing RNA binding, mRNA binding nucleus, cytoplasm 26273 chr00_6 - - 408 C 5 YJL087C TRL1 RLG1|LIG1 tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes RNA splicing, regulation of translation, response to chemical stimulus, tRNA processing, mRNA processing, signaling kinase activity, ligase activity nucleus, endomembrane system, membrane 21336 chr10_2 - - 408 C 6 YOL133W HRT1 HRT2|ROC1|RBX1 RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF mitotic cell cycle, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus, cytoplasm 26283 chr00_6 - - 408 C 7 YOL134C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit biological_process molecular_function cellular_component 26284 chr00_6 - - 408 C 8 YOL135C MED7 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription from RNA polymerase II promoter molecular_function nucleus 26285 chr00_6 - - 408 C 9 YEL034W HYP2 eIF-5A|eIF5A|TIF51A Translation elongation factor eIF-5A that may function in translation initiation; similar to and functionally redundant with Anb1p; structural homolog of bacterial EF-P; undergoes an essential hypusination modification translational initiation, regulation of translation, translational elongation RNA binding, translation factor activity, nucleic acid binding ribosome, mitochondrion, cytoplasm 26389 chr00_8 - - 408 C 10 YER093C TSC11 AVO3 Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain lipid metabolic process, cytoskeleton organization, cell wall organization or biogenesis, signaling other membrane fraction 26398 chr00_8 - - 408 C 11 YER094C PUP3 SCS32 Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 proteolysis involved in cellular protein catabolic process enzyme regulator activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 26400 chr00_8 - - 408 C 12 YHR170W NMD3 SRC5 Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex ribosomal subunit export from nucleus, nuclear transport other ribosome, cytoplasm 26418 chr00_8 - - 408 D 1 YLR033W RSC58 - Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance mitotic cell cycle, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter molecular_function nucleus 26431 chr00_8 - - 408 D 2 YMR314W PRE5 - Alpha 6 subunit of the 20S proteasome proteolysis involved in cellular protein catabolic process molecular_function nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 26454 chr00_8 - - 408 D 3 YNL244C SUI1 RFR1|MOF2 Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase translational initiation RNA binding, translation factor activity, nucleic acid binding cytoplasm 26463 chr00_8 - - 408 D 4 YNL256W FOL1 - Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities cofactor metabolic process lyase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups mitochondrial envelope, mitochondrion, cytoplasm 26466 chr00_8 - - 408 D 5 YNL260C - - Putative protein of unknown function with similarity to a human protein overexpressed in oral cancers; essential gene with defects in anaerobic and filamentous growth; localizes to the nucleus and cytoplasm biological_process molecular_function nucleus, cytoplasm 26467 chr00_8 - - 408 D 6 YNL287W SEC21 - Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 26469 chr00_8 - - 408 D 7 YOL078W AVO1 - Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth cytoskeleton organization, cell wall organization or biogenesis, signaling lipid binding, enzyme binding membrane, cytoplasm, plasma membrane 26474 chr00_8 - - 408 D 8 YPL131W RPL5 YL3|L5|L1a|RPL1|LPI14 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 26478 chr00_8 - - 408 D 9 YML091C RPM2 - Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus cytoplasmic translation, mitochondrion organization, tRNA processing, mRNA processing, transcription from RNA polymerase II promoter nuclease activity nucleus, mitochondrion, cytoplasm 26488 chr00_9 - - 408 D 10 YML092C PRE8 - Alpha 2 subunit of the 20S proteasome proteolysis involved in cellular protein catabolic process molecular_function nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 26489 chr00_9 (-met) did not mate 408 D 11 YML093W UTP14 - Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 26490 chr00_9 - - 408 D 12 YML098W TAF13 TafII19|TAF19|FUN81 TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 26496 chr00_9 - - 408 E 1 YML105C SEC65 - Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 protein targeting, protein complex biogenesis RNA binding cytoplasm 26505 chr00_9 - - 408 E 2 YML114C TAF8 TafII65|TAF65 TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 26514 chr00_9 - - 408 E 3 YML125C PGA3 NQR1 Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking other molecular_function membrane, endoplasmic reticulum, plasma membrane, cytoplasm 26526 chr00_9 - - 408 E 4 YML126C ERG13 HMGS 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis lipid metabolic process transferase activity, transferring acyl groups endoplasmic reticulum, cytoplasm 26527 chr00_9 - - 408 E 5 YML127W RSC9 - Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway mitotic cell cycle, chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity nucleus 26528 chr00_9 - - 408 E 6 YML130C ERO1 - Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum protein folding oxidoreductase activity endoplasmic reticulum, cytoplasm 26531 chr00_9 - - 408 E 7 YMR094W CTF13 CBF3C Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis cytoskeleton organization, organelle assembly, protein complex biogenesis DNA binding nucleus, chromosome 26534 chr00_9 - - 408 E 8 YMR112C MED11 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 26552 chr00_9 - - 408 E 9 YMR113W FOL3 - Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis cofactor metabolic process ligase activity cytoplasm 26553 chr00_9 - - 408 E 10 YMR117C SPC24 - Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering cytoskeleton organization, chromosome segregation structural molecule activity nucleus, chromosome 26557 chr00_9 - - 408 E 11 YMR128W ECM16 DHR1 Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis rRNA processing, ribosomal small subunit biogenesis helicase activity nucleus, nucleolus, mitochondrion, cytoplasm 26569 chr00_9 - - 408 E 12 YMR131C RRB1 - Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p other molecular_function nucleus, nucleolus 26572 chr00_9 - - 408 F 1 YMR134W - - Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes cellular ion homeostasis, mitochondrion organization molecular_function nucleus, endomembrane system, endoplasmic reticulum, cytoplasm 26575 chr00_9 - - 408 F 2 YPR016C TIF6 CDC95 Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits rRNA processing, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, nuclear transport other nucleus, nucleolus, cytoplasm 25435 chr16_4 - - 408 F 3 YPR019W MCM4 CDC54|HCD21 Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p mitotic cell cycle, DNA repair, DNA replication, response to DNA damage stimulus, DNA recombination DNA binding, ATPase activity, helicase activity nucleus, chromosome, cytoplasm 25438 chr16_4 - - 408 F 4 YPR025C CCL1 - Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters protein phosphorylation, transcription from RNA polymerase II promoter protein binding transcription factor activity, enzyme regulator activity nucleus, mitochondrion, cytoplasm 25444 chr16_4 - - 408 F 5 YPR033C HTS1 histidyl-tRNA synthetase|TSM4572 Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 25452 chr16_4 - - 408 F 6 YPR034W ARP7 RSC11|SWP61 Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity nucleus 25453 chr16_4 - - 408 F 7 YPR035W GLN1 - Glutamine synthetase (GS), synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation cellular amino acid metabolic process ligase activity cytoplasm 25454 chr16_4 - - 408 F 8 YPR041W TIF5 SUI5 Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 translational initiation, regulation of translation, organelle assembly, ribosome assembly RNA binding, enzyme regulator activity, translation factor activity, nucleic acid binding ribosome, cytoplasm 25460 chr16_4 - - 408 F 9 YPR048W TAH18 - Conserved NAPDH-dependent diflavin reductase, component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress cofactor metabolic process oxidoreductase activity mitochondrion, cytoplasm 25467 chr16_4 - - 408 F 10 YPR055W SEC8 - Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis cell budding, cytokinesis, organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, exocytosis molecular_function cell cortex, cellular bud, site of polarized growth, cytoplasm 25474 chr16_4 - - 408 F 11 YPR056W TFB4 - Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p response to DNA damage stimulus, DNA repair, protein phosphorylation, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 25475 chr16_4 - - 408 F 12 YPR082C DIB1 SNU16 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein RNA splicing, mRNA processing molecular_function nucleus 25499 chr16_4 - - 408 G 1 YPR085C ASA1 - Putative protein of unknown function; subunit of the ASTRA complex (Rvb1p, Rvb2p, Tra1p, Tti1p, Tti2, Asa1p and Tra1p) which is part of the chromatin remodeling machinery biological_process molecular_function nucleus, chromosome 25502 chr16_4 - - 408 G 2 YPR088C SRP54 SRH1 Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain protein targeting, protein complex biogenesis RNA binding cytoplasm 25505 chr16_4 - - 408 G 3 YPR094W RDS3 - Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance RNA splicing, mRNA processing, response to chemical stimulus molecular_function nucleus 25511 chr16_4 - - 408 G 4 YPR103W PRE2 SRR2|PRG1|DOA3 Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome proteolysis involved in cellular protein catabolic process, protein complex biogenesis peptidase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 25519 chr16_4 - - 408 G 5 YJL031C BET4 - Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Golgi vesicle transport, protein targeting transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 21391 chr10_2 - - 408 G 6 YJL032W - - Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase biological_process molecular_function cellular_component 21390 chr10_2 - - 408 G 7 YJL033W HCA4 ECM24|DBP4 Putative nucleolar DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis rRNA processing helicase activity, ATPase activity nucleus, nucleolus 21389 chr10_2 - - 408 G 8 YJL034W KAR2 BIP|GRP78 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p protein targeting, transmembrane transport, organelle fusion, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, conjugation, nucleus organization unfolded protein binding, ATPase activity endoplasmic reticulum, cytoplasm 21388 chr10_2 - - 408 G 9 YJL035C TAD2 - Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs tRNA processing, RNA modification hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cytoplasm 21387 chr10_2 - - 408 G 10 YJL039C NUP192 - Essential structural subunit of the nuclear pore complex (NPC), localizes to the nuclear periphery of nuclear pores, homologous to human p205 nuclear transport, nucleus organization structural molecule activity nucleus, membrane, endomembrane system 21383 chr10_2 - - 408 G 11 YJL050W MTR4 DOB1 ATP-dependent 3'-5' RNA helicase of the Dead-box family, involved in nuclear RNA processing and degradation both as a component of the TRAMP complex and in TRAMP independent processes; has a KOW domain that shows RNA binding activity snoRNA processing, rRNA processing, RNA catabolic process RNA binding, ATPase activity, helicase activity, mRNA binding nucleus, nucleolus 21373 chr10_2 - - 408 G 12 YJL054W TIM54 - Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane protein targeting, mitochondrion organization protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 21369 chr10_2 - - 408 H 1 YJL091C GWT1 - Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors lipid metabolic process, protein lipidation transferase activity, transferring acyl groups membrane, membrane fraction, endomembrane system 21332 chr10_2 met- did not mate 480 A 1 YOR160W MTR10 KAP111 Nuclear import receptor, mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs protein targeting, nucleobase-containing compound transport, nuclear transport other nucleus, membrane, cytoplasm 22416 chr15_2 FAILED QC! did not grow on dropout plates but were lys+ and met+ 480 A 2 YOR260W GCD1 TRA3 Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression translational initiation, regulation of translation RNA binding, translation factor activity, nucleic acid binding, enzyme regulator activity cytoplasm 22516 chr15_3 FAILED QC! did not grow on dropout plates but were lys+ and met+ 480 A 3 YOR319W HSH49 - U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) RNA splicing, mRNA processing RNA binding nucleus 21616 chr15_4 FAILED QC! mated as MAT alpha 480 A 4 YOR340C RPA43 A43 RNA polymerase I subunit A43 transcription from RNA polymerase I promoter nucleotidyltransferase activity nucleus, nucleolus 21637 chr15_4 FAILED QC! mated as MAT alpha 480 A 5 YOR224C RPB8 ABC14.5 RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III transcription from RNA polymerase I promoter, transcription from RNA polymerase III promoter, transcription from RNA polymerase II promoter nucleotidyltransferase activity nucleus, nucleolus 22480 chr15_3 FAILED QC! mated as MAT alpha 480 A 6 YMR211W DML1 - Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family mitochondrion organization molecular_function mitochondrion, cytoplasm 20797 chr13_4 FAILED QC! mated as MAT a 480 A 7 YOR157C PUP1 - Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z proteolysis involved in cellular protein catabolic process peptidase activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 22413 chr15_2 FAILED QC! mated as MAT a 480 A 8 YOR278W HEM4 SLU2 Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria cofactor metabolic process lyase activity cellular_component 22534 chr15_3 FAILED QC! mated as MAT a 480 A 9 YOL127W RPL25 rpl6L|YL25|L25 Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarity to E. coli L23 and rat L23a ribosomal proteins; binds to 25S rRNA via a conserved C-terminal motif cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 26277 chr00_6 FAILED QC! grew on ura and YP+5% glycerol but did not grow on YPD+G418 480 A 10 YJL090C DPB11 - Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1 mitotic cell cycle, DNA repair, protein phosphorylation, DNA replication, response to DNA damage stimulus, regulation of protein modification process, DNA recombination, regulation of cell cycle enzyme regulator activity nucleus, chromosome 21333 chr10_2 FAILED QC! grew on ura and YP+5% glycerol but did not grow on YPD+G418