mat alpha Synced to SGD on Wed Feb 1 10:00:09 2012 Plate # Row Column ORF Gene Alias Description GO Biological Process GO Molecular Function GO Cellular Component record no. go feature type r384 position 1 A 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 A 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 B 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 B 2 YLL046C RNP1 - Ribonucleoprotein that contains two RNA recognition motifs (RRM) other RNA binding cytoplasm 11534 ORF|Verified 1 - H12 1 B 3 YLR119W SRN2 VPL16|VPS37|SRN10 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus protein targeting, proteolysis involved in cellular protein catabolic process other membrane, cytoplasm 12730 ORF|Verified 2 - H1 1 B 4 YAL068C PAU8 - Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 10338 ORF|Uncharacterized 1 - A2 1 B 5 YLL051C FRE6 - Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels cellular ion homeostasis, transmembrane transport, ion transport oxidoreductase activity membrane, vacuole, cytoplasm 11539 ORF|Verified 2 - A3 1 B 6 YAL067C SEO1 - Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide other other membrane 10339 ORF|Verified 1 - A3 1 B 7 YLL052C AQY2 - Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains other transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 11540 ORF|Verified 2 - A4 1 B 8 YAL066W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10340 - 1 - A4 1 B 9 YLL053C - - Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin biological_process molecular_function membrane 11541 ORF|Uncharacterized 2 - A5 1 B 10 YAL065C - - Putative protein of unknown function; has homology to FLO1; possible pseudogene biological_process molecular_function cellular_component 10341 ORF|Uncharacterized 1 - A5 1 B 11 YLL054C - - Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene biological_process nucleic acid binding transcription factor activity, DNA binding membrane, plasma membrane 11542 ORF|Uncharacterized 2 - A6 1 B 12 YAL062W GDH3 FUN51 NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources cellular amino acid metabolic process oxidoreductase activity nucleus, mitochondrion, cytoplasm 10345 ORF|Verified 1 - A6 1 B 13 YLL055W YCT1 - High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene amino acid transport transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 11543 ORF|Verified 2 - A7 1 B 14 YAL061W BDH2 - Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity nucleus, cytoplasm 10346 ORF|Uncharacterized 1 - A7 1 B 15 YLL056C - - Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component 11544 ORF|Uncharacterized 2 - A8 1 B 16 YAL060W BDH1 (2R,3R)-2,3-butanediol dehydrogenase|BDH NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source other oxidoreductase activity cytoplasm 10347 ORF|Verified 1 - A8 1 B 17 YLL057C JLP1 - Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation other oxidoreductase activity cellular_component 11545 ORF|Verified 2 - A9 1 B 18 YAL059W ECM1 - Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm cell wall organization or biogenesis, ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus 10348 ORF|Verified 1 - A9 1 B 19 YLL058W - - Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene other transferase activity, transferring alkyl or aryl (other than methyl) groups cellular_component 11546 ORF|Uncharacterized 2 - A10 1 B 20 YAL058W CNE1 FUN48 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast protein folding, proteolysis involved in cellular protein catabolic process unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 10349 ORF|Verified 1 - A10 1 B 21 YLL060C GTT2 - Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups mitochondrion, cytoplasm 11548 ORF|Verified 2 - A11 1 B 22 YAL056W GPB2 KRH1 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; homolog of Gpb1p invasive growth in response to glucose limitation, sporulation, response to chemical stimulus, pseudohyphal growth, transcription from RNA polymerase II promoter, signaling enzyme regulator activity, signal transducer activity mitochondrion, membrane, cytoplasm, plasma membrane 10351 ORF|Verified 1 - A11 1 B 23 YLL061W MMP1 - High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p amino acid transport transmembrane transporter activity membrane, plasma membrane 11549 ORF|Verified 2 - A12 1 B 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 C 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 C 2 YML057W CMP2 CNA2 Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 cellular ion homeostasis, conjugation, signaling, response to chemical stimulus phosphatase activity cytoplasm 10513 ORF|Verified 3 - B1 1 C 3 YMR170C ALD2 - Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p cellular amino acid metabolic process oxidoreductase activity cytoplasm 10753 ORF|Verified 4 - B2 1 C 4 YML057C-A - YML058C-A Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W biological_process molecular_function cellular_component 10514 - 3 - B2 1 C 5 YMR172C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10756 - 4 - B3 1 C 6 YML056C IMD4 - Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed biological_process oxidoreductase activity cytoplasm 10515 ORF|Verified 3 - B3 1 C 7 YMR173W-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W biological_process molecular_function membrane 10758 - 4 - B4 1 C 8 YML055W SPC2 SPY1 Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 protein targeting, protein maturation peptidase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 10516 ORF|Verified 3 - B4 1 C 9 YMR174C PAI3 IA3 Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact other enzyme regulator activity cytoplasm 10759 ORF|Verified 35 - E8 1 C 10 YML054C CYB2 - Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions other oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm 10517 ORF|Verified 3 - B5 1 C 11 YMR175W SIP18 - Phospholipid-binding hydrophilin with a role in dessication resistance; expression is induced by osmotic stress response to chemical stimulus lipid binding cytoplasm 10760 ORF|Verified 35 - E9 1 C 12 YML053C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus, cytoplasm 10518 ORF|Uncharacterized 3 - B6 1 C 13 YMR176W ECM5 - Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks cell wall organization or biogenesis molecular_function nucleus, chromosome 10761 ORF|Verified 4 - B7 1 C 14 YML052W SUR7 - Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants sporulation, membrane invagination, endocytosis molecular_function cell cortex, membrane fraction, mitochondrion, cell wall, membrane, cytoplasm, plasma membrane 10519 ORF|Verified 3 - B7 1 C 15 YMR177W MMT1 - Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p cellular ion homeostasis molecular_function membrane, mitochondrion, cytoplasm 10762 ORF|Verified 4 - B8 1 C 16 YML051W GAL80 - Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding carbohydrate metabolic process, transcription from RNA polymerase II promoter transcription factor binding, enzyme regulator activity nucleus, cytoplasm 10520 ORF|Verified 3 - B8 1 C 17 YMR178W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR178W is not an essential gene biological_process molecular_function nucleus, cytoplasm 10763 ORF|Uncharacterized 4 - B9 1 C 18 YML050W AIM32 - Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 10521 ORF|Uncharacterized 3 - B9 1 C 19 YMR179W SPT21 - Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p transcription from RNA polymerase II promoter molecular_function nucleus 10764 ORF|Verified 4 - B10 1 C 20 YML048W GSF2 ECM6 ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression protein folding unfolded protein binding membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 10523 ORF|Verified 3 - B10 1 C 21 YMR180C CTL1 CTH1 RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm biological_process phosphatase activity nucleus, cytoplasm 10765 ORF|Verified 4 - B11 1 C 22 YML047W-A - YML048W-A Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 10524 - 3 - B11 1 C 23 YMR182C RGM1 - Putative transcriptional repressor with proline-rich zinc fingers; overproduction impairs cell growth transcription from RNA polymerase II promoter DNA binding nucleus 10767 ORF|Verified 4 - B12 1 C 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 D 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 D 2 YAL053W FLC2 HUF2 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance protein folding, transmembrane transport, cell wall organization or biogenesis, nucleobase-containing compound transport transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 10354 ORF|Verified 1 - B1 1 D 3 YLL063C AYT1 - Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis other transferase activity, transferring acyl groups cellular_component 11551 ORF|Verified 2 - B2 1 D 4 YAL051W OAF1 YAF1 Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis lipid metabolic process, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 10355 ORF|Verified 1 - B2 1 D 5 YLR001C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm 11556 ORF|Uncharacterized 2 - B3 1 D 6 YAL049C AIM2 - Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor biological_process molecular_function cytoplasm 10356 ORF|Uncharacterized 1 - B3 1 D 7 YLR003C CMS1 - Subunit of U3-containing 90S preribosome processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; overexpression rescues supressor mutant of mcm10; null mutant is viable ribosomal small subunit biogenesis molecular_function nucleus 11558 ORF|Uncharacterized 2 - B4 1 D 8 YAL048C GEM1 GON1 Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases mitochondrion organization, regulation of organelle organization, organelle inheritance GTPase activity, ion binding endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 10357 ORF|Verified 1 - B4 1 D 9 YLR004C THI73 - Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs other other membrane, endoplasmic reticulum, cytoplasm 11559 ORF|Verified 2 - B5 1 D 10 YAL046C AIM1 - Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 10359 ORF|Uncharacterized 1 - B5 1 D 11 YLR011W LOT6 - FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions protein targeting, transmembrane transport, regulation of transport, response to oxidative stress, response to chemical stimulus, nuclear transport oxidoreductase activity, transcription factor binding nucleus, cytoplasm 11566 ORF|Verified 2 - B6 1 D 12 YAL045C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A biological_process molecular_function cellular_component 10360 - 1 - B6 1 D 13 YLR012C - - Putative protein of unknown function; YLR012C is not an essential gene biological_process molecular_function cellular_component 11567 ORF|Uncharacterized 2 - B7 1 D 14 YAL044C GCV3 - H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 10361 ORF|Verified 1 - B7 1 D 15 YLR013W GAT3 - Protein containing GATA family zinc finger motifs other nucleic acid binding transcription factor activity, DNA binding nucleus 11568 ORF|Verified 2 - B8 1 D 16 YAL042W ERV46 FUN9 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus 10363 ORF|Verified 1 - B8 1 D 17 YLR014C PPR1 - Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p nucleobase-containing small molecule metabolic process nucleic acid binding transcription factor activity nucleus 11569 ORF|Verified 2 - B9 1 D 18 YAL042C-A - YAL043C-A Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene biological_process molecular_function cellular_component 10364 - 1 - B9 1 D 19 YLR015W BRE2 CPS60 Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L peptidyl-amino acid modification, telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 11570 ORF|Verified 2 - B10 1 D 20 YAL040C CLN3 WHI1|FUN10|DAF1 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis vacuole organization, mitotic cell cycle, protein phosphorylation, membrane fusion, regulation of protein modification process, transcription from RNA polymerase II promoter, regulation of cell cycle enzyme regulator activity nucleus 10366 ORF|Verified 1 - B10 1 D 21 YLR016C PML1 - Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p RNA splicing, mRNA processing, nucleobase-containing compound transport, nuclear transport molecular_function nucleus, cytoplasm 11571 ORF|Verified 2 - B11 1 D 22 YAL037W - - Putative protein of unknown function biological_process molecular_function cellular_component 10369 ORF|Uncharacterized 1 - B11 1 D 23 YLR017W MEU1 - Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression cellular amino acid metabolic process transferase activity, transferring glycosyl groups, RNA binding, mRNA binding cytoplasm 11572 ORF|Verified 2 - B12 1 D 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 E 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 E 2 YML034W SRC1 HEH1|YML033W Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance mitotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, endomembrane system 10537 ORF|Verified 3 - C3 1 E 3 YMR184W ADD37 - Protein of unknown function involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene proteolysis involved in cellular protein catabolic process molecular_function cytoplasm 10769 ORF|Verified 4 - C2 1 E 4 YML034C-A - YML035C-A Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10538 - 3 - C2 1 E 5 YMR185W - - Putative protein of unknown function; conflicting evidence on whether null mutant is viable with elongated buds, or inviable biological_process molecular_function cellular_component 10770 ORF|Uncharacterized 4 - C3 1 E 6 YML034W SRC1 HEH1|YML033W Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance mitotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, endomembrane system 10539 ORF|Verified 3 - C3 1 E 7 YMR186W HSC82 HSP90 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock telomere organization, protein folding, protein complex biogenesis unfolded protein binding, ATPase activity membrane fraction, mitochondrion, cytoplasm 10771 ORF|Verified 4 - C4 1 E 8 YML032C RAD52 - Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis meiotic cell cycle, DNA repair, telomere organization, response to DNA damage stimulus, DNA recombination DNA binding nucleus, chromosome 10540 ORF|Verified 3 - C4 1 E 9 YMR187C - - Putative protein of unknown function; YMR187C is not an essential gene biological_process molecular_function membrane 10772 ORF|Uncharacterized 4 - C5 1 E 10 YML030W AIM31 - Putative protein of unknown function; GFP-fusion protein localizes to mitochondria; may interact with respiratory chain complexes III or IV; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 10543 ORF|Uncharacterized 3 - C5 1 E 11 YMR188C MRPS17 - Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 10773 ORF|Verified 4 - C6 1 E 12 YML029W USA1 - Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p RNA splicing, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, mRNA processing structural molecule activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10544 ORF|Verified 3 - C6 1 E 13 YMR189W GCV2 GSD2 P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 10774 ORF|Verified 4 - C7 1 E 14 YML028W TSA1 cTPxI|TPX1|ZRG14 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype regulation of translation, response to oxidative stress, response to chemical stimulus, response to DNA damage stimulus, protein folding, regulation of cell cycle oxidoreductase activity, unfolded protein binding cytoplasm 10545 ORF|Verified 3 - C7 1 E 15 YMR190C SGS1 - Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes mitotic cell cycle, meiotic cell cycle, organelle fission, DNA replication, response to DNA damage stimulus, DNA recombination, DNA repair, telomere organization, chromosome segregation, regulation of DNA metabolic process, regulation of cell cycle helicase activity, ATPase activity nucleus, chromosome, nucleolus 10775 ORF|Verified 4 - C8 1 E 16 YML026C RPS18B S18B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins cytoplasmic translation, rRNA processing, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 10547 ORF|Verified 3 - C8 1 E 17 YMR192W GYL1 APP2 Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth regulation of transport, Golgi vesicle transport, exocytosis other mitochondrion, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 10777 ORF|Verified 4 - C9 1 E 18 YML024W RPS17A rp51A|S17A|RPL51A|RP51A Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein cytoplasmic translation, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 10549 ORF|Verified 3 - C9 1 E 19 YMR194W RPL36A YL39|L39|L36A|RPL39B N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Bp and has similarity to rat L36 ribosomal protein; binds to 5.8 S rRNA cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 10779 ORF|Verified 4 - C10 1 E 20 YML020W - - Putative protein of unknown function biological_process molecular_function cellular_component 10553 ORF|Uncharacterized 3 - C10 1 E 21 YMR193C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10780 - 4 - C11 1 E 22 YML019W OST6 - Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p peptidyl-amino acid modification, carbohydrate metabolic process, protein complex biogenesis, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10554 ORF|Verified 3 - C11 1 E 23 YMR195W ICY1 - Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation biological_process molecular_function membrane, vacuole, cytoplasm 10781 ORF|Verified 4 - C12 1 E 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 F 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 F 2 YAL035W FUN12 eIF5B|yIF2 GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 translational initiation, regulation of translation RNA binding, GTPase activity, translation factor activity, nucleic acid binding ribosome, mitochondrion, cytoplasm 10371 ORF|Verified 1 - C1 1 F 3 YLR019W PSR2 - Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p other phosphatase activity membrane, plasma membrane 11574 ORF|Verified 2 - C2 1 F 4 YAL034C FUN19 - Non-essential protein of unknown function; expression induced in response to heat stress biological_process molecular_function cellular_component 10374 ORF|Verified 1 - C2 1 F 5 YLR020C YEH2 - Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism lipid metabolic process, cell wall organization or biogenesis other membrane, plasma membrane 11575 ORF|Verified 2 - C3 1 F 6 YAL031C GIP4 FUN21 Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate chromosome segregation enzyme binding, enzyme regulator activity cytoplasm 10377 ORF|Verified 1 - C3 1 F 7 YLR021W IRC25 DMP2|PBA3|POC3 Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions RNA catabolic process, protein complex biogenesis molecular_function other 11576 ORF|Verified 2 - C4 1 F 8 YAL030W SNC1 - Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, membrane invagination, exocytosis, endocytosis other endomembrane system, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 10378 ORF|Verified 1 - C4 1 F 9 YLR023C IZH3 - Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity cellular ion homeostasis ion binding membrane, endoplasmic reticulum, cytoplasm 11578 ORF|Verified 2 - C5 1 F 10 YAL029C MYO4 SHE1|FUN22 One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p organelle inheritance cytoskeletal protein binding cellular bud, site of polarized growth, cytoskeleton, mitochondrion, cytoplasm 10379 ORF|Verified 1 - C5 1 F 11 YLR024C UBR2 - Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex protein modification by small protein conjugation or removal ligase activity cytoplasm 11579 ORF|Verified 2 - C6 1 F 12 YAL028W FRT2 HPH2 Tail-anchored ER membrane protein, interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate other molecular_function endoplasmic reticulum, cytoplasm 10380 ORF|Verified 1 - C6 1 F 13 YLR025W SNF7 VPL5|RNS4|VPS32|DID1 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes endosome transport, vesicle organization, proteolysis involved in cellular protein catabolic process, response to chemical stimulus other membrane, cytoplasm 11580 ORF|Verified 2 - C7 1 F 14 YAL026C DRS2 SWA3|FUN38 Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease organelle assembly, ribosomal small subunit biogenesis, ribosome assembly, Golgi vesicle transport, lipid transport, membrane invagination, endocytosis ATPase activity membrane, Golgi apparatus, cytoplasm 10382 ORF|Verified 1 - C7 1 F 15 YLR028C ADE16 - Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine sporulation, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm 11583 ORF|Verified 2 - C8 1 F 16 YAL023C PMT2 FUN25 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein Ser/Thr residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5; target for new antifungal carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10385 ORF|Verified 1 - C8 1 F 17 YLR042C - - Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene biological_process molecular_function cell wall 12653 ORF|Uncharacterized 2 - C9 1 F 18 YAL022C FUN26 - Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, cytoplasm 10386 ORF|Verified 1 - C9 1 F 19 YLR043C TRX1 LMA1 Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance vacuole organization, DNA replication, response to oxidative stress, response to chemical stimulus, Golgi vesicle transport, membrane fusion, organelle inheritance, regulation of DNA metabolic process oxidoreductase activity vacuole, cytoplasm 12654 ORF|Verified 2 - C10 1 F 20 YAL021C CCR4 NUT21|FUN27 Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening mitotic cell cycle, DNA replication, RNA catabolic process, transcription elongation, DNA-dependent, mRNA processing, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle nuclease activity nucleus, cytoplasm 10387 ORF|Verified 1 - C10 1 F 21 YLR044C PDC1 - Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism carbohydrate metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process lyase activity nucleus, cytoplasm 12655 ORF|Verified 2 - C11 1 F 22 YAL020C ATS1 KTI13|FUN28 Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle tRNA processing, cell budding, cytoskeleton organization, RNA modification molecular_function cytoplasm 10388 ORF|Verified 1 - C11 1 F 23 YLR046C - RLA1 Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function membrane 12657 ORF|Uncharacterized 2 - C12 1 F 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 G 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 G 2 YML017W PSP2 MRS15 Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing biological_process molecular_function cytoplasm 10556 ORF|Verified 3 - D1 1 G 3 YMR198W CIK1 - Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, chromosome segregation, conjugation, nucleus organization other cytoskeleton, microtubule organizing center, cytoplasm 10784 ORF|Verified 44 - E12 1 G 4 YML016C PPZ1 - Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis, protein dephosphorylation phosphatase activity nucleus, membrane, plasma membrane, cytoplasm 10557 ORF|Verified 3 - D2 1 G 5 YMR199W CLN1 - G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) regulation of protein modification process, protein phosphorylation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 10785 ORF|Verified 4 - D3 1 G 6 YML014W TRM9 KTI1 tRNA methyltransferase, catalyzes esterification of modified uridine nucleotides in tRNA(Arg3) and tRNA(Glu), likely as part of a complex with Trm112p; deletion confers resistance to zymocin tRNA processing, RNA modification methyltransferase activity nucleus, cytoplasm 10559 ORF|Verified 3 - D3 1 G 7 YMR201C RAD14 - Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein response to DNA damage stimulus, DNA repair DNA binding, ion binding nucleus 10787 ORF|Verified 4 - D4 1 G 8 YML013W UBX2 SEL1 Protein involved in ER-associated protein degradation; proposed to coordinate the assembly of proteins involved in ERAD; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain proteolysis involved in cellular protein catabolic process protein binding, bridging endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 10560 ORF|Verified 3 - D4 1 G 9 YMR202W ERG2 END11 C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis lipid metabolic process isomerase activity endoplasmic reticulum, cytoplasm 10788 ORF|Verified 4 - D5 1 G 10 YML012C-A - YML013C-A Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 biological_process molecular_function cellular_component 10561 - 3 - D5 1 G 11 YMR204C INP1 - Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane organelle inheritance, peroxisome organization molecular_function peroxisome, membrane, cytoplasm 10790 ORF|Verified 4 - D6 1 G 12 YML012W ERV25 - Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 10562 ORF|Verified 3 - D6 1 G 13 YMR205C PFK2 - Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes carbohydrate metabolic process, generation of precursor metabolites and energy RNA binding, kinase activity, mRNA binding mitochondrion, cytoplasm 10791 ORF|Verified 4 - D7 1 G 14 YML011C RAD33 - Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus response to DNA damage stimulus, DNA repair molecular_function nucleus 10563 ORF|Verified 3 - D7 1 G 15 YMR206W - - Putative protein of unknown function; YMR206W is not an essential gene biological_process molecular_function cellular_component 10792 ORF|Uncharacterized 4 - D8 1 G 16 YML009W-B - YML010W-A Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition biological_process molecular_function cellular_component 10565 - 3 - D8 1 G 17 YMR207C HFA1 - Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis lipid metabolic process ligase activity mitochondrion, cytoplasm 10793 ORF|Verified 4 - D9 1 G 18 YML009C MRPL39 YmL39 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 10567 ORF|Verified 35 - E1 1 G 19 YMR210W - - Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification lipid metabolic process transferase activity, transferring acyl groups cellular_component 10796 ORF|Verified 4 - D10 1 G 20 YML008C ERG6 VID1|SED6|LIS1|ISE1 Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to both lipid particles and mitochondrial outer membrane lipid metabolic process methyltransferase activity membrane, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 10568 ORF|Verified 3 - D10 1 G 21 YMR214W SCJ1 - One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins protein folding, proteolysis involved in cellular protein catabolic process, response to chemical stimulus other endoplasmic reticulum, cytoplasm 10800 ORF|Verified 4 - D11 1 G 22 YML007W YAP1 SNQ3|PAR1 Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; mediates resistance to cadmium response to heat, transcription from RNA polymerase II promoter, response to oxidative stress, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 10569 ORF|Verified 3 - D11 1 G 23 YMR215W GAS3 - Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation biological_process other cell wall, membrane, membrane fraction 10801 ORF|Verified 4 - D12 1 G 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 H 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 H 2 YAL018C - - Putative protein of unknown function biological_process molecular_function membrane 10390 ORF|Uncharacterized 1 - D1 1 H 3 YLR048W RPS0B S0B|YST2|NAB1B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal cytoplasmic translation, rRNA processing, organelle assembly, nucleobase-containing compound transport, ribosomal small subunit biogenesis, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 12659 ORF|Verified 2 - D2 1 H 4 YAL017W PSK1 FUN31 One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity cytoplasm 10391 ORF|Verified 1 - D2 1 H 5 YLR049C - - Putative protein of unknown function biological_process molecular_function cellular_component 12660 ORF|Uncharacterized 2 - D3 1 H 6 YAL015C NTG1 ogg2|SCR1|FUN33 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress DNA repair, DNA replication, mitochondrion organization, regulation of organelle organization, response to oxidative stress, response to chemical stimulus, response to DNA damage stimulus, regulation of DNA metabolic process hydrolase activity, acting on glycosyl bonds, lyase activity nucleus, mitochondrion, cytoplasm 10393 ORF|Verified 1 - D3 1 H 7 YLR053C - - Putative protein of unknown function biological_process molecular_function cellular_component 12664 ORF|Uncharacterized 2 - D4 1 H 8 YAL014C SYN8 SLT2|UIP2 Endosomal SNARE related to mammalian syntaxin 8 other other cytoplasm 10394 ORF|Verified 1 - D4 1 H 9 YLR054C OSW2 - Protein of unknown function proposed to be involved in the assembly of the spore wall sporulation, cell wall organization or biogenesis molecular_function membrane, cytoplasm 12665 ORF|Uncharacterized 2 - D5 1 H 10 YAL013W DEP1 FUN54 Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation response to heat, lipid metabolic process, chromatin organization, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, histone modification molecular_function nucleus, chromosome 10395 ORF|Verified 1 - D5 1 H 11 YLR055C SPT8 - Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 12666 ORF|Verified 2 - D6 1 H 12 YAL011W SWC3 SWC1 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae chromatin organization molecular_function nucleus, chromosome, mitochondrion, cytoplasm 10397 ORF|Verified 1 - D6 1 H 13 YLR056W ERG3 PSO6|SYR1 C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm 12667 ORF|Verified 2 - D7 1 H 14 YAL010C MDM10 FUN37 Subunit of both the ERMES complex that links the ER to mitochondria, and of the mitochondrial sorting and assembly machinery (SAM complex) that functions in import and assembly of outer membrane beta-barrel proteins protein targeting, mitochondrion organization, protein complex biogenesis, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 10398 ORF|Verified 1 - D7 1 H 15 YLR057W MNL2 - Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene proteolysis involved in cellular protein catabolic process molecular_function endoplasmic reticulum, cytoplasm 12668 ORF|Uncharacterized 2 - D8 1 H 16 YAL009W SPO7 - Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation meiotic cell cycle, lipid metabolic process, sporulation, nucleus organization phosphatase activity membrane, endomembrane system 10399 ORF|Verified 1 - D8 1 H 17 YLR058C SHM2 SHMT2 Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis other other site of polarized growth, membrane fraction, cytoplasm 12669 ORF|Verified 2 - D9 1 H 18 YAL008W FUN14 - Mitochondrial protein of unknown function biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 10400 ORF|Verified 1 - D9 1 H 19 YLR059C REX2 YNT20 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease rRNA processing, mitochondrion organization nuclease activity mitochondrion, cytoplasm 12670 ORF|Verified 2 - D10 1 H 20 YAL007C ERP2 - Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 10401 ORF|Verified 1 - D10 1 H 21 YLR061W RPL22A rp4|l1c|YL31|L22A Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 12672 ORF|Verified 2 - D11 1 H 22 YAL004W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C biological_process molecular_function cellular_component 10402 - 1 - D11 1 H 23 YLR062C BUD28 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay cytokinesis molecular_function cellular_component 12673 - 2 - D12 1 H 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 I 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 I 2 YML005W TRM12 TYW2 S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA tRNA processing, nucleobase-containing small molecule metabolic process, RNA modification methyltransferase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 10571 ORF|Verified 3 - E1 1 I 3 YMR219W ESC1 - Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p other molecular_function nucleus 10805 ORF|Verified 4 - E2 1 I 4 YML004C GLO1 - Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress cellular amino acid metabolic process lyase activity nucleus, cytoplasm 10572 ORF|Verified 3 - E2 1 I 5 YMR221C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm 10807 ORF|Uncharacterized 4 - E3 1 I 6 YML003W - - Putative protein of unknown function biological_process molecular_function cellular_component 10573 ORF|Uncharacterized 3 - E3 1 I 7 YMR222C FSH2 - Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cytoplasm 10808 ORF|Verified 4 - E4 1 I 8 YML002W - - Putative protein of unknown function; expression induced by heat and by calcium shortage biological_process molecular_function cellular_component 10574 ORF|Uncharacterized 3 - E4 1 I 9 YMR223W UBP8 - Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B protein alkylation, chromatin organization, protein modification by small protein conjugation or removal, histone modification peptidase activity, structural molecule activity nucleus 10809 ORF|Verified 4 - E5 1 I 10 YMR002W MIC17 - Mitochondrial intermembrane space protein, required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 10577 ORF|Verified 3 - E5 1 I 11 YMR224C MRE11 NGS1|XRS4|RAD58 Subunit of a complex with Rad50p and Xrs2p (MRX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for MRX function; widely conserved meiotic cell cycle, DNA repair, sporulation, response to DNA damage stimulus, DNA recombination kinase activity, DNA binding, structural molecule activity, nuclease activity nucleus, mitochondrion, cytoplasm 10810 ORF|Verified 4 - E6 1 I 12 YMR003W AIM34 - Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 10578 ORF|Uncharacterized 3 - E6 1 I 13 YMR225C MRPL44 YmL44|YMR44 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 10811 ORF|Verified 4 - E7 1 I 14 YMR006C PLB2 - Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine lipid metabolic process other cell wall, extracellular region 10581 ORF|Verified 3 - E7 1 I 15 YMR226C - TMA29 NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments other oxidoreductase activity nucleus, membrane, ribosome, cytoplasm 10812 ORF|Verified 4 - E8 1 I 16 YMR007W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10582 - 3 - E8 1 I 17 YMR230W RPS10B S10B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Ap and has similarity to rat ribosomal protein S10 cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 10816 ORF|Verified 4 - E9 1 I 18 YMR008C PLB1 - Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol lipid metabolic process other cell wall, membrane fraction 10583 ORF|Verified 3 - E9 1 I 19 YMR232W FUS2 - Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating cell morphogenesis, organelle fusion, response to chemical stimulus, conjugation, nucleus organization molecular_function nucleus, mitochondrion, site of polarized growth, cytoplasm 10818 ORF|Verified 4 - E10 1 I 20 YMR009W ADI1 - Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 10584 ORF|Verified 3 - E10 1 I 21 YMR233W TRI1 - Non-essential sumoylated protein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus biological_process molecular_function nucleus, nucleolus, cytoplasm 10819 ORF|Verified 4 - E11 1 I 22 YMR010W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 biological_process molecular_function membrane, cytoplasm 10585 ORF|Uncharacterized 3 - E11 1 I 23 YMR234W RNH1 - Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) RNA catabolic process, cell wall organization or biogenesis nuclease activity nucleus 10820 ORF|Verified 4 - E12 1 I 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 J 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 J 2 YAL002W VPS8 VPL8|VPT8|FUN15 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif protein targeting, endosome transport enzyme binding membrane fraction, cytoplasm 10405 ORF|Verified 1 - E1 1 J 3 YLR064W PER33 - Protein that localizes to the endoplasmic reticulum with some nuclear pore complex association; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p biological_process molecular_function nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 12675 ORF|Uncharacterized 2 - E2 1 J 4 YAR002W NUP60 - Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; involved in nuclear export and cytoplasmic localization of specific mRNAs such as ASH1 response to heat, DNA repair, protein targeting, nucleobase-containing compound transport, response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport lipid binding, structural molecule activity nucleus, endomembrane system, membrane 10407 ORF|Verified 1 - E2 1 J 5 YLR065C ENV10 - Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene protein maturation molecular_function membrane 12676 ORF|Uncharacterized 2 - E3 1 J 6 YAR003W SWD1 SAF49|CPS50|FUN16 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 10408 ORF|Verified 1 - E3 1 J 7 YLR068W FYV7 - Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 12679 ORF|Verified 2 - E4 1 J 8 YAR014C BUD14 - Protein involved in bud-site selection, Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cell morphogenesis, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis enzyme regulator activity cellular bud, site of polarized growth 10413 ORF|Verified 1 - E4 1 J 9 YLR072W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene biological_process molecular_function cytoplasm 12683 ORF|Uncharacterized 2 - E5 1 J 10 YAR015W ADE1 - N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process ligase activity nucleus, cytoplasm 10414 ORF|Verified 1 - E5 1 J 11 YLR073C RFU1 - Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes other enzyme regulator activity cytoplasm 12684 ORF|Uncharacterized 2 - E6 1 J 12 YAR018C KIN3 NPK1|FUN52 Nonessential serine/threonine protein kinase; possible role in DNA damage response chromosome segregation kinase activity cellular_component 10415 ORF|Verified 1 - E6 1 J 13 YLR074C BUD20 - Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cytokinesis molecular_function nucleus 12685 ORF|Verified 2 - E7 1 J 14 YAR020C PAU7 - Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component 10417 ORF|Verified 1 - E7 1 J 15 YLR077W FMP25 BCA1 Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 12688 ORF|Uncharacterized 2 - E8 1 J 16 YAR023C - - Putative integral membrane protein, member of DUP240 gene family biological_process molecular_function cellular_component 10418 ORF|Uncharacterized 1 - E8 1 J 17 YLR079W SIC1 SDB25 Inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 mitotic cell cycle, regulation of protein modification process, protein phosphorylation, response to starvation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 12690 ORF|Verified 2 - E9 1 J 18 YAR027W UIP3 - Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family biological_process molecular_function nucleus, endomembrane system 10419 ORF|Verified 1 - E9 1 J 19 YLR080W EMP46 - Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 12691 ORF|Verified 2 - E10 1 J 20 YAR028W - - Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component 10420 ORF|Uncharacterized 1 - E10 1 J 21 YLR081W GAL2 - Galactose permease, required for utilization of galactose; also able to transport glucose carbohydrate metabolic process, carbohydrate transport transmembrane transporter activity membrane, plasma membrane 12692 ORF|Verified 2 - E11 1 J 22 YAR029W - - Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm 10421 ORF|Uncharacterized 1 - E11 1 J 23 YLR082C SRL2 - Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation other molecular_function nucleus, cytoplasm 12693 ORF|Verified 2 - E12 1 J 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 K 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 K 2 YMR012W CLU1 TIF31 eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant translational initiation, mitochondrion organization molecular_function cytoplasm 10587 ORF|Verified 3 - F1 1 K 3 YMR238W DFG5 - Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p cell budding, pseudohyphal growth, cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds membrane, plasma membrane 10824 ORF|Verified 4 - F2 1 K 4 YMR014W BUD22 - Protein required for 18S rRNA maturation and small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 10589 ORF|Verified 3 - F2 1 K 5 YMR241W YHM2 - Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome mitochondrion organization, ion transport transmembrane transporter activity, DNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 10827 ORF|Verified 4 - F3 1 K 6 YMR016C SOK2 - Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors pseudohyphal growth nucleic acid binding transcription factor activity, DNA binding nucleus 10591 ORF|Verified 3 - F3 1 K 7 YMR242C RPL20A L20A|L18A|RPL18A2 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similarity to rat L18a ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 10828 ORF|Verified 4 - F4 1 K 8 YMR017W SPO20 DBI9 Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog sporulation other membrane 10592 ORF|Verified 3 - F4 1 K 9 YMR243C ZRC1 OSR1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; also has a role in resistance to zinc shock resulting from a sudden influx of zinc into the cytoplasm cellular ion homeostasis, ion transport, cellular amino acid metabolic process transmembrane transporter activity membrane, vacuole, cytoplasm 10829 ORF|Verified 4 - F5 1 K 10 YMR018W - - Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene biological_process molecular_function cellular_component 10593 ORF|Uncharacterized 3 - F5 1 K 11 YMR244W - - Putative protein of unknown function biological_process molecular_function cellular_component 10830 ORF|Uncharacterized 4 - F6 1 K 12 YMR019W STB4 - Protein that binds Sin3p in a two-hybrid assay; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters biological_process DNA binding nucleus 10594 ORF|Verified 3 - F6 1 K 13 YMR245W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10831 - 4 - F7 1 K 14 YMR020W FMS1 - Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process oxidoreductase activity cytoplasm 10595 ORF|Verified 3 - F7 1 K 15 YMR244C-A - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR244C-A is not an essential gene biological_process molecular_function nucleus, cytoplasm 10832 ORF|Uncharacterized 4 - F8 1 K 16 YMR022W UBC7 DER2|QRI8 Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly chromatin organization, proteolysis involved in cellular protein catabolic process, cell wall organization or biogenesis ligase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10597 ORF|Verified 3 - F8 1 K 17 YMR246W FAA4 - Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles lipid transport, cofactor metabolic process ligase activity cytoplasm 10833 ORF|Verified 4 - F9 1 K 18 YMR023C MSS1 PET53 Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 mitochondrion organization, tRNA processing, nucleobase-containing small molecule metabolic process, RNA modification other mitochondrion, membrane, mitochondrial envelope, cytoplasm 10598 ORF|Verified 3 - F9 1 K 19 YMR247C RKR1 LTN1 RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity nucleus, ribosome, cytoplasm 10834 ORF|Verified 4 - F10 1 K 20 YMR024W MRPL3 YmL3 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 10599 ORF|Verified 3 - F10 1 K 21 YMR250W GAD1 - Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress response to oxidative stress, response to chemical stimulus, cellular amino acid metabolic process lyase activity cytoplasm 10835 ORF|Verified 4 - F11 1 K 22 YMR025W CSI1 - Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling protein modification by small protein conjugation or removal, conjugation, signaling, response to chemical stimulus molecular_function nucleus 10600 ORF|Verified 3 - F11 1 K 23 YMR251W GTO3 - Omega class glutathione transferase; putative cytosolic localization cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 10836 ORF|Verified 4 - F12 1 K 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 L 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 L 2 YAR030C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 biological_process molecular_function cellular_component 10423 - 1 - F1 1 L 3 YLR084C RAX2 - N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p cytokinesis molecular_function cellular bud, cell wall, membrane, site of polarized growth, mitochondrion, cytoplasm 12695 ORF|Verified 2 - F2 1 L 4 YAR035W YAT1 - Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated cellular amino acid metabolic process transferase activity, transferring acyl groups mitochondrion, cytoplasm 10425 ORF|Verified 1 - F2 1 L 5 YLR085C ARP6 - Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin organization other nucleus, chromosome, cytoplasm 12696 ORF|Verified 2 - F3 1 L 6 YAR037W - - - - - - 10426 - 1 - F3 1 L 7 YLR087C CSF1 - Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies generation of precursor metabolites and energy molecular_function mitochondrion, cytoplasm 12698 ORF|Verified 2 - F4 1 L 8 YAR040C - - - - - - 10427 - 1 - F4 1 L 9 YLR089C ALT1 - Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cellular amino acid metabolic process other mitochondrion, cytoplasm 12700 ORF|Uncharacterized 2 - F5 1 L 10 YAR042W SWH1 OSH1|YAR044W Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction lipid transport, membrane invagination, exocytosis, endocytosis lipid binding nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm 10428 ORF|Verified 1 - F5 1 L 11 YLR090W XDJ1 - Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein folding other nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm 12701 ORF|Verified 2 - F6 1 L 12 YAR043C-A - - - - - - 10429 - 1 L 13 YLR092W SUL2 - High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates ion transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 12703 ORF|Verified 2 - F7 1 L 14 YAR042W SWH1 OSH1|YAR044W Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction lipid transport, membrane invagination, exocytosis, endocytosis lipid binding nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm 10430 ORF|Verified 1 - F5 1 L 15 YLR093C NYV1 MAM2 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane vacuole organization, organelle fusion, vesicle organization, membrane fusion other vacuole, membrane, cytoplasm 12704 ORF|Verified 2 - F8 1 L 16 YAR047C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10431 - 1 - F8 1 L 17 YLR094C GIS3 - Protein of unknown function signaling molecular_function nucleus, cytoplasm 12705 ORF|Verified 2 - F9 1 L 18 YLL001W DNM1 - Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance organelle fission, mitochondrion organization, peroxisome organization, protein complex biogenesis, organelle inheritance GTPase activity peroxisome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 11489 ORF|Verified 1 - F9 1 L 19 YLR095C IOC2 - Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif chromatin organization DNA binding, ATPase activity nucleus 12706 ORF|Verified 2 - F10 1 L 20 YLL002W RTT109 KAT11|REM50|KIM2 Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, transposition, histone modification, response to DNA damage stimulus, transcription from RNA polymerase II promoter transferase activity, transferring acyl groups nucleus 11490 ORF|Verified 1 - F10 1 L 21 YLR096W KIN2 - Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p protein phosphorylation, exocytosis kinase activity membrane, plasma membrane 12707 ORF|Verified 2 - F11 1 L 22 YLL005C SPO75 - Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis sporulation, cell wall organization or biogenesis molecular_function membrane 11493 ORF|Verified 1 - F11 1 L 23 YLR097C HRT3 - Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies proteolysis involved in cellular protein catabolic process, response to chemical stimulus protein binding, bridging, ligase activity other 12708 ORF|Verified 2 - F12 1 L 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 M 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 M 2 YMR027W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene biological_process molecular_function nucleus, cytoplasm 10602 ORF|Uncharacterized 3 - G1 1 M 3 YMR252C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 10838 ORF|Uncharacterized 4 - G2 1 M 4 YMR029C FAR8 - Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p conjugation, signaling, response to chemical stimulus molecular_function cellular_component 10604 ORF|Verified 3 - G2 1 M 5 YMR253C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene biological_process molecular_function membrane, cytoplasm 10839 ORF|Uncharacterized 4 - G3 1 M 6 YMR030W RSF1 - Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes mitochondrion organization, generation of precursor metabolites and energy, cellular respiration, transcription from RNA polymerase II promoter, signaling molecular_function nucleus, mitochondrion, cytoplasm 10605 ORF|Verified 3 - G3 1 M 7 YMR254C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10840 - 4 - G4 1 M 8 YMR031W-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C biological_process molecular_function cellular_component 10606 - 3 - G4 1 M 9 YMR255W GFD1 - Coiled-coiled protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation nucleobase-containing compound transport, nuclear transport molecular_function nucleus, membrane, endomembrane system, cytoplasm 10841 ORF|Verified 4 - G5 1 M 10 YMR031C EIS1 - Component of the eisosome that is required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies protein complex biogenesis molecular_function membrane, cell cortex, mitochondrion, cytoplasm 10607 ORF|Uncharacterized 3 - G5 1 M 11 YMR258C ROY1 - GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p protein targeting, membrane invagination, nucleobase-containing small molecule metabolic process, endocytosis enzyme regulator activity nucleus, membrane fraction, cytoplasm 10844 ORF|Uncharacterized 4 - G6 1 M 12 YMR032W HOF1 CYK2 Bud neck-localized, SH3 domain-containing protein required for cytokinesis; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p cytokinesis, response to DNA damage stimulus cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 10608 ORF|Verified 3 - G6 1 M 13 YMR259C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene biological_process molecular_function cytoplasm 10845 ORF|Uncharacterized 4 - G7 1 M 14 YMR034C - - Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene biological_process other membrane 10610 ORF|Uncharacterized 3 - G7 1 M 15 YMR261C TPS3 - Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, phosphatase activity, enzyme regulator activity cytoplasm 10847 ORF|Verified 4 - G8 1 M 16 YMR036C MIH1 - Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 mitotic cell cycle, regulation of protein modification process, protein phosphorylation, regulation of cell cycle phosphatase activity nucleus, cytoplasm 10612 ORF|Verified 3 - G8 1 M 17 YMR262W - - Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene biological_process molecular_function cellular_component 10848 ORF|Uncharacterized 4 - G9 1 M 18 YMR039C SUB1 TSP1 Transcriptional coactivator, facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes transcription from RNA polymerase III promoter, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter, response to osmotic stress protein binding transcription factor activity nucleus 10615 ORF|Verified 3 - G9 1 M 19 YMR263W SAP30 - Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance transcription from RNA polymerase I promoter, response to heat, transcription from RNA polymerase II promoter hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, chromosome 10849 ORF|Verified 4 - G10 1 M 20 YMR040W YET2 - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function membrane, ribosome, cytoplasm 10616 ORF|Verified 3 - G10 1 M 21 YMR264W CUE1 KIS4 Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination proteolysis involved in cellular protein catabolic process enzyme regulator activity membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 10850 ORF|Verified 4 - G11 1 M 22 YMR041C ARA2 - NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase other oxidoreductase activity cellular_component 10617 ORF|Verified 3 - G11 1 M 23 YMR265C - - Putative protein of unknown function biological_process molecular_function cellular_component 10851 ORF|Uncharacterized 4 - G12 1 M 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 N 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 N 2 YLL012W YEH1 - Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes lipid metabolic process other membrane, cytoplasm 11500 ORF|Verified 1 - G1 1 N 3 YLR099C ICT1 - Lysophosphatidic acid acyltransferase, responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress lipid metabolic process transferase activity, transferring acyl groups cellular_component 12710 ORF|Verified 2 - G2 1 N 4 YLL013C PUF3 - Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins mitochondrion organization, generation of precursor metabolites and energy, cellular respiration, RNA catabolic process RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 11501 ORF|Verified 1 - G2 1 N 5 YLR102C APC9 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition chromatin organization, protein modification by small protein conjugation or removal ligase activity nucleus 12713 ORF|Verified 2 - G3 1 N 6 YLL014W EMC6 - Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 11502 ORF|Uncharacterized 1 - G3 1 N 7 YLR104W LCL2 - Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) proteolysis involved in cellular protein catabolic process molecular_function cellular_component 12715 ORF|Uncharacterized 2 - G4 1 N 8 YLL015W BPT1 - ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p ion transport transmembrane transporter activity membrane, vacuole, cytoplasm 11503 ORF|Verified 1 - G4 1 N 9 YLR107W REX3 - RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases rRNA processing nuclease activity nucleus, cytoplasm 12718 ORF|Verified 2 - G5 1 N 10 YLL016W - - - - - - 11504 - 1 - G5 1 N 11 YLR108C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene biological_process molecular_function nucleus 12719 ORF|Uncharacterized 2 - G6 1 N 12 YLL017W - - - - - - 11505 - 1 - G6 1 N 13 YLR109W AHP1 cTPxIII Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p response to oxidative stress, response to chemical stimulus oxidoreductase activity membrane fraction, cytoplasm 12720 ORF|Verified 2 - G7 1 N 14 YLL019C KNS1 L124 Nonessential putative protein kinase of unknown cellular role; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues protein phosphorylation kinase activity cellular_component 11507 ORF|Verified 1 - G7 1 N 15 YLR111W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 12722 - 2 - G8 1 N 16 YLL020C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 biological_process molecular_function cellular_component 11508 - 1 - G8 1 N 17 YLR112W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 12723 - 2 - G9 1 N 18 YLL021W SPA2 PEA1|FUS6 Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate cell morphogenesis, cell budding, cytoskeleton organization, cytokinesis, response to chemical stimulus, conjugation, pseudohyphal growth, signaling cytoskeletal protein binding cell cortex, cellular bud, site of polarized growth, cytoplasm 11509 ORF|Verified 1 - G9 1 N 19 YLR113W HOG1 SSK3 Mitogen-activated protein kinase involved in osmoregulation; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p response to heat, protein phosphorylation, response to chemical stimulus, transcription from RNA polymerase II promoter, signaling, response to osmotic stress kinase activity, signal transducer activity nucleus, cytoplasm 12724 ORF|Verified 2 - G10 1 N 20 YLL023C POM33 - Transmembrane nucleoporin involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p nucleus organization molecular_function nucleus, endomembrane system, ribosome, membrane, endoplasmic reticulum, cytoplasm 11511 ORF|Verified 1 - G10 1 N 21 YLR114C AVL9 - Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms Golgi vesicle transport molecular_function cytoplasm 12725 ORF|Verified 2 - G11 1 N 22 YLL024C SSA2 YG102 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes protein targeting, transmembrane transport, protein folding unfolded protein binding, ATPase activity membrane fraction, vacuole, mitochondrion, cell wall, membrane, cytoplasm 11512 ORF|Verified 1 - G11 1 N 23 YLR118C - APT1 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS other other nucleus, cytoplasm 12729 ORF|Verified 2 - G12 1 N 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 O 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 O 2 YMR044W IOC4 - Member of a complex (Isw1b) with Isw1p and Ioc2p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PWWP motif chromatin organization DNA binding, ATPase activity nucleus 10620 ORF|Verified 3 - H1 1 O 3 YMR272C SCS7 FAH1 Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm 10858 ORF|Verified 4 - H2 1 O 4 YMR140W SIP5 - Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase response to starvation molecular_function cytoplasm 10721 ORF|Verified 3 - H2 1 O 5 YMR156C TPP1 - DNA 3'-phosphatase that functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; has similarity to the l-2-haloacid dehalogenase superfamily response to DNA damage stimulus, DNA repair phosphatase activity cellular_component 10738 ORF|Verified 4 - A1 1 O 6 YML058W SML1 - Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase response to DNA damage stimulus, mitochondrion organization enzyme regulator activity nucleus, cytoplasm 10512 ORF|Verified 3 - A12 1 O 7 YMR273C ZDS1 OSS1|NRC1|CKM1|CES1 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at the telomere; has a role in Bcy1p localization; implicated in mRNA nuclear export mitotic cell cycle, nucleobase-containing compound transport, nuclear transport, regulation of cell cycle enzyme regulator activity nucleus, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm 10859 ORF|Verified 4 - H4 1 O 8 YMR141C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10722 - 3 - H4 1 O 9 YMR274C RCE1 - Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone protein maturation peptidase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 10860 ORF|Verified 4 - H5 1 O 10 YMR143W RPS16A rp61R|S16A Protein component of the small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosomal proteins cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 10724 ORF|Verified 3 - H5 1 O 11 YMR275C BUL1 SMM2|RDS1|DAG1 Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases protein modification by small protein conjugation or removal, organelle inheritance, mitochondrion organization ligase activity membrane, plasma membrane 10861 ORF|Verified 4 - H6 1 O 12 YMR144W - - Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene biological_process molecular_function nucleus 10725 ORF|Uncharacterized 3 - H6 1 O 13 YMR276W DSK2 - Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome cytoskeleton organization, proteolysis involved in cellular protein catabolic process protein binding, bridging nucleus 10862 ORF|Verified 4 - H7 1 O 14 YMR145C NDE1 NDH1 Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy oxidoreductase activity mitochondrion, cytoplasm 10726 ORF|Verified 3 - H7 1 O 15 YMR278W PGM3 - Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential biological_process isomerase activity nucleus, cytoplasm 10864 ORF|Verified 4 - H8 1 O 16 YMR147W - - Putative protein of unknown function biological_process molecular_function cellular_component 10728 ORF|Uncharacterized 3 - H8 1 O 17 YMR280C CAT8 MSP8|DIL1 Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements carbohydrate metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 10866 ORF|Verified 4 - H9 1 O 18 YMR148W OSW5 - Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion biological_process molecular_function membrane 10729 ORF|Uncharacterized 3 - H9 1 O 19 YMR283C RIT1 initiator methionine tRNA 2'-O-ribosyl phosphate transferase 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA tRNA processing, RNA modification transferase activity, transferring glycosyl groups cytoplasm 10869 ORF|Verified 4 - H10 1 O 20 YMR153W NUP53 - Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex mitotic cell cycle, protein targeting, organelle fission, regulation of organelle organization, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport, regulation of cell cycle, nucleus organization lipid binding, DNA binding, structural molecule activity nucleus, endomembrane system, membrane 10734 ORF|Verified 3 - H10 1 O 21 YMR284W YKU70 KU70|NES24|HDF1 Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair DNA repair, telomere organization, chromatin organization, response to DNA damage stimulus, DNA recombination RNA binding, DNA binding nucleus, endomembrane system, chromosome 10870 ORF|Verified 4 - H11 1 O 22 YMR153C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 biological_process molecular_function cellular_component 10735 - 3 - H11 1 O 23 YMR285C NGL2 - Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p and Ngl3p rRNA processing nuclease activity other 10871 ORF|Verified 4 - H12 1 O 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 1 YOR128C ADE2 - Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process lyase activity cytoplasm 12384 ORF|Verified 42 - E4 1 P 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 1 P 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 A 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 B 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 B 2 YMR289W ABZ2 - Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process lyase activity cytoplasm 10875 ORF|Verified 5 - A1 2 B 3 YOR021C - - Putative protein of unknown function; YOR021C is not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism biological_process molecular_function cellular_component 11797 ORF|Uncharacterized 6 - A2 2 B 4 YMR291W TDA1 - Putative kinase of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process kinase activity nucleus, cytoplasm 10878 ORF|Uncharacterized 5 - A2 2 B 5 YOR022C - - Protein with similarity to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other mitochondrion, cytoplasm 11798 ORF|Uncharacterized 6 - A3 2 B 6 YMR292W GOT1 - Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Golgi vesicle transport, endosome transport molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 10879 ORF|Verified 5 - A3 2 B 7 YOR023C AHC1 - Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups nucleus 11799 ORF|Verified 6 - A4 2 B 8 YMR294W JNM1 PAC3|INS1 Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B mitotic cell cycle, cytoskeleton organization structural molecule activity cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 10881 ORF|Verified 5 - A4 2 B 9 YOR024W - IRC12 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11800 - 6 - A5 2 B 10 YOR018W ROD1 ART4 Membrane protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene,zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins membrane invagination, response to chemical stimulus, endocytosis enzyme binding membrane, plasma membrane 11794 ORF|Verified 5 - H12 2 B 11 YOR312C RPL20B L20B|L18B|RPL18A1 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similarity to rat L18a ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 11609 ORF|Verified 6 - H1 2 B 12 YMR294W-A - - Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function cellular_component 10882 - 5 - A6 2 B 13 YOR025W HST3 - Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism lipid metabolic process, chromatin organization, histone modification hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, DNA binding nucleus 11801 ORF|Verified 6 - A7 2 B 14 YMR295C - - Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene biological_process molecular_function cellular bud, site of polarized growth, ribosome, cytoplasm 10883 ORF|Verified 5 - A7 2 B 15 YOR026W BUB3 - Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle small conjugating protein binding nucleus, chromosome 11802 ORF|Verified 6 - A8 2 B 16 YMR297W PRC1 CPY|LBC1 Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family other peptidase activity vacuole, endoplasmic reticulum, cytoplasm 10885 ORF|Verified 5 - A8 2 B 17 YOR027W STI1 - Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop protein folding RNA binding, mRNA binding, enzyme regulator activity cytoplasm 11803 ORF|Verified 6 - A9 2 B 18 YMR299C DYN3 - Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration other other cytoskeleton, cytoplasm 10887 ORF|Verified 5 - A9 2 B 19 YOR028C CIN5 YAP4|HAL6 Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions transcription from RNA polymerase II promoter, response to osmotic stress, response to chemical stimulus DNA binding, transcription factor binding nucleus, cytoplasm 11804 ORF|Verified 6 - A10 2 B 20 YMR300C ADE4 - Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups cytoplasm 10888 ORF|Verified 5 - A10 2 B 21 YOR029W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11805 - 6 - A11 2 B 22 YMR302C YME2 PRP12|RNA12 Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 10890 ORF|Verified 5 - A11 2 B 23 YOR030W DFG16 ZRG12|ECM41 Probable multiple transmembrane protein, involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; required for accumulation of processed Rim101p pseudohyphal growth, protein maturation molecular_function membrane 11806 ORF|Verified 6 - A12 2 B 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 C 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 C 2 YOR348C PUT4 - Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells amino acid transport transmembrane transporter activity membrane, plasma membrane 11645 ORF|Verified 7 - B1 2 C 3 YOL085C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A biological_process molecular_function cellular_component 11776 - 8 - B2 2 C 4 YOR349W CIN1 - Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl protein folding cytoskeletal protein binding cellular_component 11646 ORF|Verified 7 - B2 2 C 5 YPL274W SAM3 - High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p transmembrane transport, amino acid transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 11018 ORF|Verified 8 - B3 2 C 6 YOR350C MNE1 - Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA RNA splicing molecular_function mitochondrion, cytoplasm 11647 ORF|Verified 7 - B3 2 C 7 YPL273W SAM4 - S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process methyltransferase activity nucleus, cytoplasm 11019 ORF|Verified 8 - B4 2 C 8 YOR351C MEK1 MRE4 Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids meiotic cell cycle, protein phosphorylation, regulation of cell cycle kinase activity nucleus 11648 ORF|Verified 7 - B4 2 C 9 YPL272C - - Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene biological_process molecular_function cellular_component 11020 ORF|Uncharacterized 8 - B5 2 C 10 YOR352W - - Putative protein of unknown function; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm 11649 ORF|Uncharacterized 7 - B5 2 C 11 YPL270W MDL2 - Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis transmembrane transport ATPase activity, transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 11022 ORF|Verified 8 - B6 2 C 12 YOR354C MSC6 - Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies meiotic cell cycle, DNA recombination molecular_function mitochondrion, cytoplasm 11651 ORF|Verified 7 - B6 2 C 13 YPL269W KAR9 - Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase other molecular_function cell cortex, site of polarized growth, cytoskeleton, microtubule organizing center, cytoplasm 11023 ORF|Verified 8 - B7 2 C 14 YOR355W GDS1 - Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrion, cytoplasm 11652 ORF|Verified 7 - B7 2 C 15 YPL267W ACM1 - Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein mitotic cell cycle, regulation of protein modification process, protein modification by small protein conjugation or removal enzyme regulator activity nucleus, cytoplasm 11025 ORF|Verified 8 - B8 2 C 16 YOR356W CIR2 - Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response biological_process molecular_function mitochondrion, cytoplasm 11653 ORF|Verified 7 - B8 2 C 17 YPL265W DIP5 - Dicarboxylic amino acid permease, mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly amino acid transport transmembrane transporter activity membrane, plasma membrane 11027 ORF|Verified 8 - B9 2 C 18 YOR357C SNX3 GRD19 Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Golgi vesicle transport, endosome transport lipid binding cytoplasm 11654 ORF|Verified 7 - B9 2 C 19 YPL264C - - Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene biological_process molecular_function membrane 11028 ORF|Uncharacterized 8 - B10 2 C 20 YOR358W HAP5 - Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, response to chemical stimulus, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus 11655 ORF|Verified 7 - B10 2 C 21 YPL263C KEL3 - Cytoplasmic protein of unknown function biological_process molecular_function cytoplasm 11029 ORF|Verified 8 - B11 2 C 22 YOR359W VTS1 - Post-transcriptional gene regulator, flap-structured DNA-binding and RNA-binding protein; shows genetic interactions with Vti1p, a v-SNARE involved in cis-Golgi membrane traffic; stimulates Dna2p endonuclease activity; contains a SAM domain RNA catabolic process, mRNA processing RNA binding, DNA binding nucleus, cytoplasm 11656 ORF|Verified 7 - B11 2 C 23 YPL262W FUM1 - Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration lyase activity mitochondrion, cytoplasm 11030 ORF|Verified 8 - B12 2 C 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 D 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 D 2 YMR304W UBP15 - Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p protein modification by small protein conjugation or removal peptidase activity cytoplasm 10892 ORF|Verified 5 - B1 2 D 3 YOR032C HMS1 - Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant pseudohyphal growth nucleic acid binding transcription factor activity cellular_component 11808 ORF|Verified 6 - B2 2 D 4 YMR304C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 biological_process molecular_function cellular_component 10893 - 5 - B2 2 D 5 YOR033C EXO1 DHS1 5'-3' exonuclease and flap-endonuclease involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains response to DNA damage stimulus, meiotic cell cycle, DNA repair, telomere organization, DNA recombination nuclease activity nucleus 11809 ORF|Verified 6 - B3 2 D 6 YMR305C SCW10 - Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p conjugation hydrolase activity, acting on glycosyl bonds cell wall, extracellular region, endoplasmic reticulum, cytoplasm 10894 ORF|Verified 5 - B3 2 D 7 YOR034C AKR2 - Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p protein acylation, membrane invagination, endocytosis, protein lipidation transferase activity, transferring acyl groups membrane, Golgi apparatus, cytoplasm 11810 ORF|Verified 6 - B4 2 D 8 YMR306C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10896 - 5 - B4 2 D 9 YOR035C SHE4 DIM1 Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization cytoskeleton organization cytoskeletal protein binding cytoplasm 11811 ORF|Verified 6 - B5 2 D 10 YMR307W GAS1 GGP1|CWH52 Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery cell wall organization or biogenesis other nucleus, membrane fraction, mitochondrion, cell wall, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm, plasma membrane 10897 ORF|Verified 5 - B5 2 D 11 YOR036W PEP12 VPT13|VPS6|VPL6 Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin vacuole organization, Golgi vesicle transport, organelle inheritance other cytoplasm, Golgi apparatus 11812 ORF|Verified 6 - B6 2 D 12 YMR310C - - Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene biological_process methyltransferase activity nucleus 10900 ORF|Uncharacterized 5 - B6 2 D 13 YOR037W CYC2 - Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) mitochondrion organization, protein complex biogenesis oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 11813 ORF|Verified 6 - B7 2 D 14 YNL339C YRF1-6 YRF1 Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p telomere organization, DNA recombination helicase activity nucleus 11105 ORF|Verified 5 - B7 2 D 15 YOR038C HIR2 SPT1 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p chromatin organization, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, DNA binding nucleus 11814 ORF|Verified 6 - B8 2 D 16 YNL338W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV biological_process molecular_function cellular_component 11106 - 5 - B8 2 D 17 YOR039W CKB2 CK2 subunit Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase transcription from RNA polymerase I promoter, response to DNA damage stimulus, protein phosphorylation, transcription from RNA polymerase III promoter enzyme regulator activity nucleus, nucleolus 11815 ORF|Verified 6 - B9 2 D 18 YNL336W COS1 - Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function membrane, vacuole, cytoplasm 11108 ORF|Verified 5 - B9 2 D 19 YOR040W GLO4 - Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate carbohydrate metabolic process other mitochondrion, cytoplasm 11816 ORF|Verified 6 - B10 2 D 20 YNL335W DDI3 - Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants biological_process molecular_function cellular_component 11109 ORF|Uncharacterized 5 - B10 2 D 21 YOR041C - SRF5 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component 11817 - 6 - B11 2 D 22 YNL334C SNO2 - Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin vitamin metabolic process molecular_function cellular_component 11110 ORF|Verified 5 - B11 2 D 23 YOR042W CUE5 - Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process small conjugating protein binding cytoplasm 11818 ORF|Verified 6 - B12 2 D 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 E 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 E 2 YOR363C PIP2 OAF2 Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes lipid metabolic process, transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, membrane 11660 ORF|Verified 7 - C1 2 E 3 YPL261C - - Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W biological_process molecular_function cellular_component 11032 - 8 - C2 2 E 4 YOR365C - - Putative protein of unknown function; YOR365C is not an essential protein biological_process molecular_function membrane 11662 ORF|Uncharacterized 7 - C2 2 E 5 YPL259C APM1 YAP54 Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 11033 ORF|Verified 8 - C3 2 E 6 YOR367W SCP1 - Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin cytoskeleton organization protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm 11664 ORF|Verified 7 - C3 2 E 7 YPL258C THI21 - Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p vitamin metabolic process kinase activity cellular_component 11034 ORF|Verified 8 - C4 2 E 8 YOR368W RAD17 - Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins meiotic cell cycle, DNA repair, response to DNA damage stimulus, DNA recombination, regulation of cell cycle DNA binding nucleus, chromosome 11665 ORF|Verified 7 - C4 2 E 9 YPL257W - - Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene biological_process molecular_function membrane 11035 ORF|Uncharacterized 8 - C5 2 E 10 YOR371C GPB1 KRH2 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; homolog of Gpb2p invasive growth in response to glucose limitation, proteolysis involved in cellular protein catabolic process, response to chemical stimulus, protein modification by small protein conjugation or removal, pseudohyphal growth, signaling enzyme regulator activity, signal transducer activity membrane, cytoplasm, plasma membrane 11668 ORF|Verified 7 - C5 2 E 11 YPL256C CLN2 - G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) protein phosphorylation, response to chemical stimulus, regulation of protein modification process, conjugation, signaling, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 11036 ORF|Verified 8 - C6 2 E 12 YOR374W ALD4 ALDH2|ALD7 Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, cytoplasm 11671 ORF|Verified 7 - C6 2 E 13 YPL254W HFI1 GAN1|SRM12|SUP110|ADA1 Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification protein binding transcription factor activity nucleus 11038 ORF|Verified 8 - C7 2 E 14 YOR376W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. biological_process molecular_function membrane 11673 - 7 - C7 2 E 15 YPL253C VIK1 - Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p mitotic cell cycle, organelle fission, chromosome segregation other cytoskeleton, microtubule organizing center, cytoplasm 11039 ORF|Verified 8 - C8 2 E 16 YOR377W ATF1 - Alcohol acetyltransferase with potential roles in lipid and sterol metabolism; responsible for the major part of volatile acetate ester production during fermentation lipid metabolic process, generation of precursor metabolites and energy transferase activity, transferring acyl groups endomembrane system, cytoplasm 11674 ORF|Verified 7 - C8 2 E 17 YPL250C ICY2 - Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate other molecular_function cellular_component 11042 ORF|Verified 8 - C9 2 E 18 YOR378W - - Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene biological_process molecular_function membrane 11675 ORF|Uncharacterized 7 - C9 2 E 19 YPL249C GYP5 - GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth Golgi vesicle transport enzyme regulator activity membrane fraction, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 11043 ORF|Verified 8 - C10 2 E 20 YOR380W RDR1 - Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 11677 ORF|Verified 7 - C10 2 E 21 YPL248C GAL4 GAL81 DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p carbohydrate metabolic process, response to chemical stimulus, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus 11044 ORF|Verified 8 - C11 2 E 22 YOR381W FRE3 - Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels cellular ion homeostasis, ion transport oxidoreductase activity membrane, plasma membrane 11678 ORF|Verified 7 - C11 2 E 23 YPL247C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest biological_process molecular_function nucleus, cytoplasm 11045 ORF|Uncharacterized 8 - C12 2 E 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 F 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 F 2 YNL332W THI12 - Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 vitamin metabolic process molecular_function cellular_component 11112 ORF|Verified 5 - C1 2 F 3 YOR044W IRC23 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endoplasmic reticulum, cytoplasm 11820 ORF|Uncharacterized 6 - C2 2 F 4 YNL329C PEX6 PAS8 AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol protein targeting, peroxisome organization, signaling ATPase activity peroxisome, membrane fraction, cytoplasm 11115 ORF|Verified 5 - C2 2 F 5 YOR045W TOM6 MOM8B|ISP6 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex protein targeting, mitochondrion organization, transmembrane transport, protein complex biogenesis protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 11821 ORF|Verified 6 - C3 2 F 6 YNL328C MDJ2 - Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, enzyme regulator activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 11116 ORF|Verified 5 - C3 2 F 7 YOR047C STD1 SFS3|MSN3 Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] carbohydrate metabolic process, nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter, response to osmotic stress, signaling enzyme regulator activity nucleus, membrane, plasma membrane 11823 ORF|Verified 6 - C4 2 F 8 YNL327W EGT2 - Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner cytokinesis hydrolase activity, acting on glycosyl bonds cellular bud, cell wall 11117 ORF|Verified 5 - C4 2 F 9 YOR049C RSB1 - Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane lipid transport ATPase activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 11825 ORF|Verified 6 - C5 2 F 10 YNL326C PFA3 - Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions vacuole organization, protein acylation, membrane fusion, protein lipidation transferase activity, transferring acyl groups vacuole, membrane, cytoplasm 11118 ORF|Verified 5 - C5 2 F 11 YOR050C - YOR29-01 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable biological_process molecular_function cellular_component 11826 - 6 - C6 2 F 12 YNL324W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11119 - 5 - C6 2 F 13 YOR051C ETT1 NRO1|YOR29-02 Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough other molecular_function nucleus 11827 ORF|Uncharacterized 6 - C7 2 F 14 YNL325C FIG4 - Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain lipid metabolic process phosphatase activity membrane, vacuole, cytoplasm 11120 ORF|Verified 5 - C7 2 F 15 YOR052C - YOR29-03 Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic biological_process molecular_function nucleus 11828 ORF|Verified 6 - C8 2 F 16 YNL323W LEM3 ROS3|BRE3 Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane lipid transport, signaling ATPase activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 11121 ORF|Verified 5 - C8 2 F 17 YOR053W - YOR29-04 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C biological_process molecular_function cellular_component 11829 - 6 - C9 2 F 18 YNL322C KRE1 - Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor cell wall organization or biogenesis structural molecule activity cell wall, membrane 11122 ORF|Verified 5 - C9 2 F 19 YOR054C VHS3 YOR29-05 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis cofactor metabolic process, cellular ion homeostasis, nucleobase-containing small molecule metabolic process lyase activity, enzyme regulator activity cytoplasm 11830 ORF|Verified 6 - C10 2 F 20 YNL321W VNX1 - Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate ion transport transmembrane transporter activity membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm 11123 ORF|Verified 5 - C10 2 F 21 YOR055W - YOR29-06 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11831 - 6 - C11 2 F 22 YNL320W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 11124 ORF|Uncharacterized 5 - C11 2 F 23 YOR058C ASE1 YOR29-09 Mitotic spindle midzone localized microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle assembly, cytokinesis, protein complex biogenesis, regulation of cell cycle cytoskeletal protein binding nucleus, cytoskeleton 11834 ORF|Verified 6 - C12 2 F 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 G 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 G 2 YOR383C FIT3 - Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall cellular ion homeostasis, ion transport molecular_function cell wall 11680 ORF|Verified 7 - D1 2 G 3 YPL245W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus, cytoplasm 11047 ORF|Uncharacterized 8 - D2 2 G 4 YOR384W FRE5 - Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process oxidoreductase activity membrane, mitochondrion, cytoplasm 11681 ORF|Verified 7 - D2 2 G 5 YPL244C HUT1 - Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 transmembrane transport, carbohydrate transport transmembrane transporter activity membrane 11048 ORF|Verified 8 - D3 2 G 6 YOR385W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene biological_process molecular_function cytoplasm 11682 ORF|Uncharacterized 7 - D3 2 G 7 YPL241C CIN2 - GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 protein folding enzyme regulator activity cellular_component 11051 ORF|Verified 8 - D4 2 G 8 YOR386W PHR1 - DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p DNA repair, response to DNA damage stimulus RNA binding, lyase activity, mRNA binding nucleus, mitochondrion, cytoplasm 11683 ORF|Verified 7 - D4 2 G 9 YPL240C HSP82 HSP90 Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p telomere organization, protein targeting, DNA replication, mitochondrion organization, regulation of organelle organization, protein complex biogenesis, protein folding, regulation of DNA metabolic process, response to osmotic stress unfolded protein binding, ATPase activity cytoplasm 11052 ORF|Verified 8 - D5 2 G 10 YOL001W PHO80 phoR|VAC5|TUP7|AGS3 Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling protein phosphorylation, cellular ion homeostasis, regulation of protein modification process, response to starvation, transcription from RNA polymerase II promoter, regulation of cell cycle, signaling enzyme regulator activity nucleus 11692 ORF|Verified 7 - D5 2 G 11 YPL239W YAR1 - Cytoplasmic ankyrin-repeat containing protein of unknown function, proposed to link the processes of 40S ribosomal subunit biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock ribosomal small subunit biogenesis, response to oxidative stress, response to osmotic stress, response to chemical stimulus molecular_function cytoplasm 11053 ORF|Verified 8 - D6 2 G 12 YOL002C IZH2 PHO36 Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc lipid metabolic process, cellular ion homeostasis, response to chemical stimulus ion binding membrane, plasma membrane 11693 ORF|Verified 7 - D6 2 G 13 YPL236C ENV7 - Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; has sequence similarity with the human serine/threonine protein kinase STK16 protein maturation kinase activity membrane, vacuole, cytoplasm 11056 ORF|Uncharacterized 8 - D7 2 G 14 YOL003C PFA4 - Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases protein acylation, protein lipidation transferase activity, transferring acyl groups membrane, endoplasmic reticulum, cytoplasm 11694 ORF|Verified 7 - D7 2 G 15 YPL234C VMA11 TFP3|CLS9 Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 11058 ORF|Verified 8 - D8 2 G 16 YOL004W SIN3 UME4|SDS16|SDI1|RPD1|GAM2|CPE1 Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity transcription from RNA polymerase I promoter, response to heat, mitotic cell cycle, meiotic cell cycle, DNA repair, chromatin organization, DNA replication, histone modification, response to DNA damage stimulus, regulation of DNA metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus, chromosome 11695 ORF|Verified 7 - D8 2 G 17 YPL232W SSO1 - Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p sporulation, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion lipid binding membrane fraction, vacuole, membrane, cytoplasm, plasma membrane 11060 ORF|Verified 8 - D9 2 G 18 YOL006C TOP1 MAK17|MAK1 Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination mitotic cell cycle, organelle fission, chromatin organization, DNA replication, chromosome segregation, DNA recombination, transcription elongation, DNA-dependent, regulation of DNA metabolic process, transcription from RNA polymerase II promoter isomerase activity, DNA binding nucleus, chromosome, nucleolus 11697 ORF|Verified 7 - D9 2 G 19 YPL230W USV1 NSF1 Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis transcription from RNA polymerase II promoter, response to osmotic stress, response to chemical stimulus DNA binding nucleus 11062 ORF|Verified 8 - D10 2 G 20 YOL007C CSI2 - Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene biological_process molecular_function cellular bud, site of polarized growth, vacuole, cytoplasm 11698 ORF|Verified 7 - D10 2 G 21 YPL229W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL229W is not an essential gene biological_process molecular_function cytoplasm 11063 ORF|Uncharacterized 8 - D11 2 G 22 YOL008W COQ10 - Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes generation of precursor metabolites and energy, cellular respiration other membrane, mitochondrial envelope, mitochondrion, cytoplasm 11699 ORF|Verified 7 - D11 2 G 23 YPL227C ALG5 - UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 11065 ORF|Verified 8 - D12 2 G 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 H 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 H 2 YNL318C HXT14 - Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies carbohydrate transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 11126 ORF|Verified 5 - D1 2 H 3 YOR061W CKA2 CK2 subunit|YOR29-12 Alpha' catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase transcription from RNA polymerase I promoter, response to DNA damage stimulus, protein phosphorylation, transcription from RNA polymerase III promoter kinase activity nucleus, nucleolus 11837 ORF|Verified 6 - D2 2 H 4 YNL314W DAL82 - Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus 11130 ORF|Verified 5 - D2 2 H 5 YOR062C - YOR29-13 Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm 11838 ORF|Uncharacterized 6 - D3 2 H 6 YNL311C SKP2 - F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments proteolysis involved in cellular protein catabolic process, cellular amino acid metabolic process protein binding, bridging, ligase activity ribosome, cytoplasm 11133 ORF|Verified 5 - D3 2 H 7 YOR064C YNG1 YOR29-15 Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification histone binding, transferase activity, transferring acyl groups nucleus 11840 ORF|Verified 6 - D4 2 H 8 YNL307C MCK1 CMS1|YPK1 Protein serine/threonine/tyrosine (dual-specificity) kinase involved in control of chromosome segregation and in regulating entry into meiosis; related to mammalian glycogen synthase kinases of the GSK-3 family mitotic cell cycle, meiotic cell cycle, DNA repair, protein phosphorylation, organelle fission, sporulation, chromosome segregation, response to DNA damage stimulus kinase activity other 11137 ORF|Verified 5 - D4 2 H 9 YOR066W MSA1 YOR29-17 Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation mitotic cell cycle, transcription from RNA polymerase II promoter molecular_function nucleus, cytoplasm 11842 ORF|Uncharacterized 6 - D5 2 H 10 YNL305C BXI1 YBH3 Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p response to chemical stimulus, signaling molecular_function vacuole, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 11139 ORF|Uncharacterized 5 - D5 2 H 11 YOR067C ALG8 YOR29-18 Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p carbohydrate metabolic process, protein glycosylation, lipid metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 11843 ORF|Verified 6 - D6 2 H 12 YNL304W YPT11 - Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells organelle inheritance, mitochondrion organization GTPase activity cellular bud, site of polarized growth, endoplasmic reticulum, cytoplasm 11140 ORF|Verified 5 - D6 2 H 13 YOR068C VAM10 YOR29-19 Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering vacuole organization, membrane fusion molecular_function membrane, vacuole, cytoplasm 11844 ORF|Verified 6 - D7 2 H 14 YNL303W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11141 - 5 - D7 2 H 15 YOR069W VPS5 YOR29-20|VPT5|PEP10|GRD2 Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p endosome transport protein transporter activity, lipid binding membrane, endomembrane system, cytoplasm 11845 ORF|Verified 6 - D8 2 H 16 YNL302C RPS19B rp55B|YS16B|S19B|S16aB|RP55B Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Ap cytoplasmic translation, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 11142 ORF|Verified 5 - D8 2 H 17 YOR070C GYP1 YOR29-21 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion other enzyme regulator activity mitochondrion, cytoplasm, Golgi apparatus 11846 ORF|Verified 6 - D9 2 H 18 YNL301C RPL18B rp28B|L18B|RP28B Protein component of the large (60S) ribosomal subunit, identical to Rpl18Ap and has similarity to rat L18 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 11143 ORF|Verified 5 - D9 2 H 19 YOR071C NRT1 THI71|YOR29-22 High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 11847 ORF|Verified 6 - D10 2 H 20 YNL299W TRF5 - Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p RNA catabolic process, snoRNA processing nucleotidyltransferase activity nucleus, nucleolus 11145 ORF|Verified 5 - D10 2 H 21 YOR072W - YOR29-23 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive biological_process molecular_function cellular_component 11848 - 6 - D11 2 H 22 YNL298W CLA4 ERC10 Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p vacuole organization, mitotic cell cycle, transmembrane transport, response to chemical stimulus, lipid transport, cell budding, protein phosphorylation, cytokinesis, organelle inheritance, regulation of cell cycle, signaling kinase activity nucleus, vacuole, cellular bud, cytoplasm 11146 ORF|Verified 5 - D11 2 H 23 YOR073W SGO1 YOR29-24 Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle molecular_function nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm 11849 ORF|Verified 6 - D12 2 H 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 I 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 I 2 YOL012C HTZ1 H2AZ|H2A.F/Z|HTA3 Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin RNA catabolic process, transcription from RNA polymerase II promoter other nucleus, chromosome 11703 ORF|Verified 7 - E1 2 I 3 YPL225W - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function ribosome, cytoplasm 11067 ORF|Verified 8 - E2 2 I 4 YOL013C HRD1 DER3 Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger cell wall organization or biogenesis, proteolysis involved in cellular protein catabolic process ligase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 11704 ORF|Verified 7 - E2 2 I 5 YPL223C GRE1 - Hydrophilin of unknown function; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway biological_process molecular_function cytoplasm 11069 ORF|Verified 8 - E3 2 I 6 YOL014W - - Putative protein of unknown function biological_process molecular_function cellular_component 11705 ORF|Uncharacterized 7 - E3 2 I 7 YPL222W FMP40 - Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 11070 ORF|Uncharacterized 8 - E4 2 I 8 YOL015W IRC10 - Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component 11706 ORF|Uncharacterized 7 - E4 2 I 9 YPL221W FLC1 BOP1|HUF1 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance nucleobase-containing compound transport, protein folding, cell wall organization or biogenesis transmembrane transporter activity vacuole, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm 11071 ORF|Verified 8 - E5 2 I 10 YOL017W ESC8 - Protein involved in telomeric and mating-type locus silencing, interacts with Sir2p and also interacts with the Gal11p, which is a component of the RNA pol II mediator complex other molecular_function nucleus 11708 ORF|Verified 7 - E5 2 I 11 YPL220W RPL1A L1A|SSM1 N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 11072 ORF|Verified 8 - E6 2 I 12 YOL018C TLG2 - Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) protein targeting, organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion, lipid metabolic process, membrane invagination, endocytosis other membrane, Golgi apparatus, cytoplasm 11709 ORF|Verified 7 - E6 2 I 13 YPL219W PCL8 - Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other 11073 ORF|Verified 8 - E7 2 I 14 YOL019W - TOS7 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole biological_process molecular_function membrane, vacuole, plasma membrane, cytoplasm 11710 ORF|Uncharacterized 7 - E7 2 I 15 YPL216W - - Putative protein of unknown function; YPL216W is not an essential gene biological_process molecular_function cellular_component 11076 ORF|Uncharacterized 8 - E8 2 I 16 YOL020W TAT2 TAP2|SCM2|SAB2|LTG3 High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance amino acid transport transmembrane transporter activity membrane, plasma membrane 11711 ORF|Verified 7 - E8 2 I 17 YPL214C THI6 - Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern vitamin metabolic process kinase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm 11078 ORF|Verified 8 - E9 2 I 18 YOL023W IFM1 - Mitochondrial translation initiation factor 2 translational initiation, mitochondrial translation, mitochondrion organization RNA binding, GTPase activity, translation factor activity, nucleic acid binding mitochondrion, cytoplasm 11714 ORF|Verified 7 - E9 2 I 19 YPL213W LEA1 - Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein RNA splicing, mRNA processing molecular_function nucleus, cytoplasm 11079 ORF|Verified 8 - E10 2 I 20 YOL024W - - Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site biological_process molecular_function cellular_component 11715 ORF|Uncharacterized 7 - E10 2 I 21 YPL212C PUS1 - tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA tRNA processing, RNA modification isomerase activity nucleus 11080 ORF|Verified 8 - E11 2 I 22 YOL025W LAG2 ECM36 Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 regulation of protein modification process, protein modification by small protein conjugation or removal molecular_function mitochondrion, cytoplasm 11716 ORF|Verified 7 - E11 2 I 23 YPL208W RKM1 - SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit protein L23a (RPL23A and RPL23B) peptidyl-amino acid modification, protein alkylation methyltransferase activity nucleus, cytoplasm 11084 ORF|Verified 8 - E12 2 I 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 J 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 J 2 YNL297C MON2 YSL2 Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins protein targeting, Golgi vesicle transport, membrane invagination, endosome transport, endocytosis enzyme regulator activity membrane, Golgi apparatus, cytoplasm 11148 ORF|Verified 5 - E1 2 J 3 YOR078W BUD21 YOR29-29|UTP16 Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 11854 ORF|Verified 6 - E2 2 J 4 YNL295W - - Putative protein of unknown function biological_process molecular_function cellular_component 11149 ORF|Uncharacterized 5 - E2 2 J 5 YOR079C ATX2 YOR29-30 Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation cellular ion homeostasis transmembrane transporter activity membrane, endomembrane system, cytoplasm, Golgi apparatus 11855 ORF|Verified 6 - E3 2 J 6 YNL294C RIM21 PAL2 Component of the RIM101 pathway, has a role in cell wall construction and alkaline pH response; has similarity to A. nidulans PalH invasive growth in response to glucose limitation, sporulation, cell wall organization or biogenesis molecular_function membrane 11150 ORF|Verified 5 - E3 2 J 7 YOR080W DIA2 YOR29-31 Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth invasive growth in response to glucose limitation, DNA replication, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process ligase activity, DNA binding nucleus, chromosome 11856 ORF|Verified 6 - E4 2 J 8 YNL293W MSB3 GYP3 GTPase-activating protein for Sec4p and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth exocytosis, cytoskeleton organization enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoplasm 11151 ORF|Verified 5 - E4 2 J 9 YOR081C TGL5 STC2 Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate lipid metabolic process transferase activity, transferring acyl groups cytoplasm 11857 ORF|Verified 6 - E5 2 J 10 YNL292W PUS4 - Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) tRNA processing, RNA modification isomerase activity nucleus, mitochondrion, cytoplasm 11152 ORF|Verified 5 - E5 2 J 11 YOR082C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W biological_process molecular_function membrane 11858 - 6 - E6 2 J 12 YNL291C MID1 - N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer ion transport transmembrane transporter activity membrane, endoplasmic reticulum, plasma membrane, cytoplasm 11153 ORF|Verified 5 - E6 2 J 13 YOR083W WHI5 - Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 mitotic cell cycle, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 11859 ORF|Verified 6 - E7 2 J 14 YNL289W PCL1 HCS26 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth mitotic cell cycle, regulation of protein modification process, protein phosphorylation, cytoskeleton organization, response to starvation, regulation of cell cycle enzyme regulator activity nucleus 11155 ORF|Verified 5 - E7 2 J 15 YOR084W LPX1 - Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction peroxisome organization other peroxisome, cytoplasm 11860 ORF|Verified 6 - E8 2 J 16 YNL288W CAF40 - Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p transcription from RNA polymerase II promoter molecular_function nucleus 11156 ORF|Verified 5 - E8 2 J 17 YOR085W OST3 - Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins carbohydrate metabolic process, protein complex biogenesis, protein glycosylation transferase activity, transferring glycosyl groups endomembrane system, membrane, endoplasmic reticulum, cytoplasm 11861 ORF|Verified 6 - E9 2 J 18 YNL286W CUS2 - Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) RNA splicing, mRNA processing RNA binding nucleus 11158 ORF|Verified 5 - E9 2 J 19 YOR086C TCB1 - Lipid-binding ER protein, enriched at ER-plasma membrane contact sites; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding membrane, cell cortex, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm 11862 ORF|Verified 6 - E10 2 J 20 YNL285W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11159 - 5 - E10 2 J 21 YOR087W YVC1 TRPY1|YOR088W Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 11863 ORF|Verified 6 - E12 2 J 22 YNL283C WSC2 STA3 Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response response to heat, cytoskeleton organization, cell wall organization or biogenesis, signaling signal transducer activity membrane fraction, cellular bud, site of polarized growth, cytoplasm 11161 ORF|Verified 5 - E11 2 J 23 YOR087W YVC1 TRPY1|YOR088W Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 11864 ORF|Verified 6 - E12 2 J 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 K 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 K 2 YOL028C YAP7 - Putative basic leucine zipper (bZIP) transcription factor transcription from RNA polymerase II promoter DNA binding nucleus 11719 ORF|Verified 7 - F1 2 K 3 YPL206C PGC1 - Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs lipid metabolic process other mitochondrion, cytoplasm 11086 ORF|Uncharacterized 8 - F2 2 K 4 YOL029C - - Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function cellular_component 11720 ORF|Uncharacterized 7 - F2 2 K 5 YPL205C - - Hypothetical protein; deletion of locus affects telomere length sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 11087 - 8 - F3 2 K 6 YOL030W GAS5 - 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall biological_process other cell wall, membrane, membrane fraction 11721 ORF|Verified 7 - F3 2 K 7 YPL203W TPK2 PKA3|YKR1|PKA2 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase protein phosphorylation, invasive growth in response to glucose limitation, signaling kinase activity nucleus 11089 ORF|Verified 8 - F4 2 K 8 YOL031C SIL1 SLS1 Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein protein targeting, transmembrane transport enzyme regulator activity endoplasmic reticulum, cytoplasm 11722 ORF|Verified 7 - F4 2 K 9 YPL202C AFT2 - Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; similar to Aft1p response to oxidative stress, response to chemical stimulus, cellular ion homeostasis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, mitochondrion, cytoplasm 11090 ORF|Verified 8 - F5 2 K 10 YOL032W OPI10 - Protein with a possible role in phospholipid biosynthesis, based on inositol-excreting phenotype of the null mutant and its suppression by exogenous choline carbohydrate metabolic process molecular_function nucleus, cytoplasm 11723 ORF|Verified 7 - F5 2 K 11 YPL201C YIG1 - Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol carbohydrate metabolic process, generation of precursor metabolites and energy molecular_function nucleus, cytoplasm 11091 ORF|Verified 8 - F6 2 K 12 YOL035C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11726 - 7 - F6 2 K 13 YPL200W CSM4 - Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements meiotic cell cycle, chromosome segregation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, endomembrane system, chromosome, membrane, endoplasmic reticulum, cytoplasm 11092 ORF|Verified 8 - F7 2 K 14 YOL036W - - Protein of unknown function; potential Cdc28p substrate biological_process molecular_function cellular_component 11727 ORF|Uncharacterized 7 - F7 2 K 15 YPL199C - - Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cytoplasm 11093 ORF|Uncharacterized 8 - F8 2 K 16 YOL037C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W biological_process molecular_function cellular_component 11728 - 7 - F8 2 K 17 YPL198W RPL7B YL8|L7B|L6B|rp11 Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) cytoplasmic translation structural molecule activity, structural constituent of ribosome nucleus, nucleolus, ribosome, cytoplasm 11094 ORF|Verified 8 - F9 2 K 18 YOL039W RPP2A RPLA2|RPL44 Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 11730 ORF|Verified 7 - F9 2 K 19 YPL197C - - Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B biological_process molecular_function cellular_component 11095 - 8 - F10 2 K 20 YOL041C NOP12 - Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p rRNA processing, ribosomal large subunit biogenesis RNA binding nucleus, nucleolus 11732 ORF|Verified 7 - F10 2 K 21 YPL196W OXR1 - Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes response to oxidative stress, response to chemical stimulus molecular_function mitochondrion, cytoplasm 11096 ORF|Verified 8 - F11 2 K 22 YOL042W NGL1 - Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process nuclease activity mitochondrion, cytoplasm 11733 ORF|Verified 7 - F11 2 K 23 YPL195W APL5 YKS4 Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Golgi vesicle transport other membrane, cytoplasm 11097 ORF|Verified 8 - F12 2 K 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 L 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 L 2 YNL280C ERG24 - C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions lipid metabolic process oxidoreductase activity membrane, endoplasmic reticulum, cytoplasm 11164 ORF|Verified 5 - F1 2 L 3 YOR090C PTC5 PPP1 Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p protein dephosphorylation phosphatase activity mitochondrion, cytoplasm 11866 ORF|Verified 6 - F2 2 L 4 YNL278W CAF120 - Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation transcription from RNA polymerase II promoter molecular_function nucleus 11166 ORF|Verified 5 - F2 2 L 5 YOR091W TMA46 RBF46 Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p cytoplasmic translation molecular_function ribosome, cytoplasm 11867 ORF|Verified 6 - F3 2 L 6 YNL275W BOR1 - Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 protein targeting, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 11169 ORF|Verified 5 - F3 2 L 7 YOR092W ECM3 YOR3165W Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation cell wall organization or biogenesis ATPase activity membrane, endoplasmic reticulum, cytoplasm 11868 ORF|Verified 6 - F4 2 L 8 YNL273W TOF1 - Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase mitotic cell cycle, DNA repair, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 11171 ORF|Verified 5 - F4 2 L 9 YOR093C - - Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function membrane 11869 ORF|Uncharacterized 6 - F5 2 L 10 YNL271C BNI1 SHE5|PPF3 Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 mitotic cell cycle, cytoskeleton organization, regulation of organelle organization, cytokinesis, protein complex biogenesis other cell cortex, membrane fraction, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 11173 ORF|Verified 5 - F5 2 L 11 YOR094W ARF3 ARL2 Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity cytoskeleton organization, cytokinesis, lipid metabolic process, membrane invagination, endocytosis RNA binding, GTPase activity, mRNA binding cellular bud, site of polarized growth 11870 ORF|Verified 6 - F6 2 L 12 YNL270C ALP1 APL1 Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression amino acid transport transmembrane transporter activity membrane, plasma membrane 11174 ORF|Verified 5 - F6 2 L 13 YOR289W - - Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 11585 ORF|Uncharacterized 6 - F7 2 L 14 YNL269W BSC4 - Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p biological_process molecular_function cellular_component 11175 ORF|Verified 5 - F7 2 L 15 YER070W RNR1 SDS12|RIR1|CRT7 Major isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits nucleobase-containing small molecule metabolic process oxidoreductase activity cytoplasm 10210 ORF|Verified 6 - F8 2 L 16 YNL266W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C biological_process molecular_function cellular_component 11178 - 5 - F8 2 L 17 YOR291W YPK9 - Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome biological_process molecular_function membrane, vacuole, cytoplasm 11587 ORF|Uncharacterized 6 - F9 2 L 18 YNL265C IST1 - Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p endosome transport molecular_function cytoplasm 11179 ORF|Verified 5 - F9 2 L 19 YOR292C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 11588 ORF|Uncharacterized 6 - F10 2 L 20 YNL264C PDR17 SFH4|ISS1 Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition lipid transport, lipid metabolic process, response to chemical stimulus other cytoplasm 11180 ORF|Verified 5 - F10 2 L 21 YOR293W RPS10A S10A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Bp and has similarity to rat ribosomal protein S10 cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 11589 ORF|Verified 6 - F11 2 L 22 YNL259C ATX1 - Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake cellular ion homeostasis, ion transport, response to oxidative stress, response to chemical stimulus ion binding cytoplasm 11185 ORF|Verified 5 - F11 2 L 23 YOR295W UAF30 - Subunit of UAF (upstream activation factor), which is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate transcription from RNA polymerase I promoter, chromatin organization nucleic acid binding transcription factor activity, DNA binding nucleus, nucleolus 11591 ORF|Verified 6 - F12 2 L 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 M 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 M 2 YOL044W PEX15 PAS21 Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly protein targeting, peroxisome organization, signaling other peroxisome, membrane, cytoplasm 11735 ORF|Verified 7 - G1 2 M 3 YPL193W RSA1 - Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus 11099 ORF|Verified 8 - G2 2 M 4 YOL045W PSK2 - One of two (see also PSK1) PAS domain containing S/T protein kinases; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation) carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity mitochondrion, cytoplasm 11736 ORF|Verified 7 - G2 2 M 5 YPL192C PRM3 - Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body organelle fusion, membrane fusion, conjugation, nucleus organization molecular_function nucleus, cytoskeleton, microtubule organizing center, endomembrane system, membrane, cytoplasm 11100 ORF|Verified 8 - G3 2 M 6 YOL046C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W biological_process molecular_function cellular_component 11737 - 7 - G3 2 M 7 YPL191C - - Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 11101 ORF|Uncharacterized 8 - G4 2 M 8 YOL047C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function membrane, cytoplasm 11738 ORF|Uncharacterized 7 - G4 2 M 9 YPL189W GUP2 - Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog carbohydrate transport transferase activity, transferring acyl groups membrane 11103 ORF|Verified 44 - F6 2 M 10 YOL048C RRT8 - Putative protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles biological_process molecular_function membrane, cytoplasm 11739 ORF|Uncharacterized 7 - G5 2 M 11 YPL188W POS5 - Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to oxidative stress, response to chemical stimulus kinase activity mitochondrion, cytoplasm 11104 ORF|Verified 8 - G6 2 M 12 YOL049W GSH2 - Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock cellular amino acid metabolic process ligase activity other 11740 ORF|Verified 7 - G6 2 M 13 YPL187W MF(ALPHA)1 alpha factor Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor conjugation, signaling, response to chemical stimulus other extracellular region 12065 ORF|Verified 8 - G7 2 M 14 YOL050C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) biological_process molecular_function cellular_component 11741 - 7 - G7 2 M 15 YPL185W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C biological_process molecular_function membrane 12066 - 8 - G8 2 M 16 YOL051W GAL11 MED15|ABE1|SPT13|SDS4|RAR3 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 11742 ORF|Verified 7 - G8 2 M 17 YPL186C UIP4 - Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope biological_process molecular_function nucleus, membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 12067 ORF|Verified 8 - G9 2 M 18 YOL052C SPE2 - S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process lyase activity nucleus, cytoplasm 11743 ORF|Verified 7 - G9 2 M 19 YPL184C MRN1 PTR69 RNA-binding protein proposed to be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES) biological_process molecular_function cytoplasm 12068 ORF|Uncharacterized 8 - G10 2 M 20 YOL052C-A DDR2 YOL053C-A|DDRA2 Multistress response protein, expression is activated by a variety of xenobiotic agents and environmental or physiological stresses other molecular_function vacuole, cytoplasm 11744 ORF|Verified 7 - G10 2 M 21 YPL181W CTI6 RXT1 Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain DNA replication, regulation of DNA metabolic process histone binding, transcription factor binding nucleus, chromosome 12070 ORF|Verified 8 - G11 2 M 22 YOL053W AIM39 - Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 11745 ORF|Uncharacterized 7 - G11 2 M 23 YPL182C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W biological_process molecular_function cellular_component 12071 - 8 - G12 2 M 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 N 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 N 2 YNL255C GIS2 - Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 regulation of translation RNA binding, DNA binding, mRNA binding ribosome, cytoplasm 11189 ORF|Verified 5 - G1 2 N 3 YOR297C TIM18 - Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex protein targeting, mitochondrion organization, response to oxidative stress, response to chemical stimulus, response to osmotic stress protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 11593 ORF|Verified 6 - G2 2 N 4 YNL254C RTC4 - Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 11190 ORF|Uncharacterized 5 - G2 2 N 5 YOR298W MUM3 - Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases lipid metabolic process, sporulation, cell wall organization or biogenesis transferase activity, transferring acyl groups cellular_component 11594 ORF|Verified 6 - G3 2 N 6 YNL253W TEX1 - Protein involved in mRNA export, component of the transcription export (TREX) complex nucleobase-containing compound transport, nuclear transport molecular_function nucleus 11191 ORF|Verified 5 - G3 2 N 7 YOR299W BUD7 - Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane carbohydrate metabolic process, Golgi vesicle transport, cytokinesis, cell wall organization or biogenesis molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 11595 ORF|Verified 6 - G4 2 N 8 YNL249C MPA43 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 11195 ORF|Verified 5 - G4 2 N 9 YOR301W RAX1 - Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily cytokinesis molecular_function cellular bud, membrane, site of polarized growth 11597 ORF|Verified 6 - G5 2 N 10 YNL248C RPA49 A49 RNA polymerase I subunit A49 transcription from RNA polymerase I promoter, transcription elongation, DNA-dependent molecular_function nucleus, nucleolus 11196 ORF|Verified 5 - G5 2 N 11 YOR302W - - CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation other cytoplasm 11598 ORF|Verified 45 - A7 2 N 12 YNL246W VPS75 - NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, regulation of organelle organization, histone modification, response to DNA damage stimulus, regulation of protein modification process histone binding, enzyme regulator activity nucleus, chromosome 11198 ORF|Verified 5 - G6 2 N 13 YOR303W CPA1 - Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader cellular amino acid metabolic process ligase activity cytoplasm 11599 ORF|Verified 45 - A8 2 N 14 YOR001W RRP6 - Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay RNA catabolic process, snoRNA processing, rRNA processing nuclease activity nucleus, nucleolus 11777 ORF|Verified 5 - G7 2 N 15 YOR304C-A - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck biological_process molecular_function cellular bud, site of polarized growth, cytoplasm 11600 ORF|Uncharacterized 6 - G8 2 N 16 YOR002W ALG6 - Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease carbohydrate metabolic process, generation of precursor metabolites and energy, cellular respiration, protein glycosylation, lipid metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 11778 ORF|Verified 5 - G8 2 N 17 YOR304W ISW2 - ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth chromatin organization, transcription termination, DNA-dependent, transcription from RNA polymerase II promoter, response to chemical stimulus DNA binding, ATPase activity nucleus 11601 ORF|Verified 6 - G9 2 N 18 YOR003W YSP3 - Putative precursor to the subtilisin-like protease III other peptidase activity cellular_component 11779 ORF|Verified 5 - G9 2 N 19 YOR307C SLY41 - Protein involved in ER-to-Golgi transport Golgi vesicle transport molecular_function membrane, endoplasmic reticulum, cytoplasm 11604 ORF|Verified 6 - G10 2 N 20 YOR005C DNL4 LIG4 DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth response to DNA damage stimulus, DNA repair ligase activity nucleus, chromosome 11781 ORF|Verified 5 - G10 2 N 21 YOR308C SNU66 - Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 RNA splicing, rRNA processing, mRNA processing molecular_function nucleus 11605 ORF|Verified 6 - G11 2 N 22 YOR006C TSR3 - Protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, cytoplasm 11782 ORF|Uncharacterized 5 - G11 2 N 23 YOR311C DGK1 HSD1 Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain lipid metabolic process kinase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 11608 ORF|Verified 6 - G12 2 N 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 O 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 O 2 YOL055C THI20 - Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p vitamin metabolic process kinase activity, hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cytoplasm 11747 ORF|Verified 7 - H1 2 O 3 YPL179W PPQ1 SAL6 Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors regulation of translation, protein dephosphorylation phosphatase activity cytoplasm 12073 ORF|Verified 8 - H2 2 O 4 YOL056W GPM3 - Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process molecular_function, isomerase activity cytoplasm 11748 ORF|Verified 7 - H2 2 O 5 YOL067C RTG1 - Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus transcription from RNA polymerase II promoter, signaling, response to chemical stimulus nucleic acid binding transcription factor activity nucleus, cytoplasm 11759 ORF|Verified 8 - A1 2 O 6 YOR347C PYK2 - Pyruvate kinase that appears to be modulated by phosphorylation; PYK2 transcription is repressed by glucose, and Pyk2p may be active under low glycolytic flux other kinase activity mitochondrion, cytoplasm 11644 ORF|Verified 7 - A12 2 O 7 YPL178W CBC2 CBP20|SAE1|MUD13 Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif RNA splicing, mRNA processing RNA binding nucleus 12074 ORF|Verified 8 - H4 2 O 8 YOL057W - - Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers other peptidase activity nucleus, cytoplasm 11749 ORF|Uncharacterized 7 - H4 2 O 9 YPL177C CUP9 - Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription regulation of transport, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus 12075 ORF|Verified 8 - H5 2 O 10 YOL058W ARG1 ARG10 Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate cellular amino acid metabolic process ligase activity cytoplasm 11750 ORF|Verified 7 - H5 2 O 11 YPL176C TRE1 - Plasma membrane protein that binds to Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; function is redundant with that of Tre2p; has similarity to transferrin receptors protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process molecular_function vacuole, cytoplasm 12076 ORF|Verified 8 - H6 2 O 12 YOL059W GPD2 GPD3 NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, cytoplasm 11751 ORF|Verified 7 - H6 2 O 13 YPL174C NIP100 PAC13 Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) mitotic cell cycle, cytoskeleton organization cytoskeletal protein binding cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 12078 ORF|Verified 8 - H7 2 O 14 YOL060C MAM3 - Protein required for normal mitochondrial morphology, has similarity to hemolysins cellular ion homeostasis, mitochondrion organization molecular_function membrane, vacuole, cytoplasm 11752 ORF|Verified 7 - H7 2 O 15 YPL171C OYE3 Old Yellow Enzyme|ZRG6 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death other oxidoreductase activity cellular_component 12081 ORF|Verified 8 - H8 2 O 16 YOL061W PRS5 - 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes carbohydrate metabolic process, cell wall organization or biogenesis other cytoplasm 11753 ORF|Verified 7 - H8 2 O 17 YPL170W DAP1 - Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis lipid metabolic process ion binding membrane, cytoplasm 12082 ORF|Verified 8 - H9 2 O 18 YOL062C APM4 AMP1 Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, plasma membrane, cytoplasm 11754 ORF|Verified 7 - H9 2 O 19 YPL168W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated biological_process molecular_function mitochondrion, cytoplasm 12084 ORF|Uncharacterized 8 - H10 2 O 20 YOL063C CRT10 HUS1 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat transcription from RNA polymerase II promoter molecular_function cellular_component 11755 ORF|Verified 7 - H10 2 O 21 YPL167C REV3 PSO1 Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair response to DNA damage stimulus, DNA repair nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm 12085 ORF|Verified 8 - H11 2 O 22 YOL064C MET22 3'(2')5'-bisphosphate nucleotidase|HAL2 Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway response to osmotic stress, cellular amino acid metabolic process phosphatase activity cellular_component 11756 ORF|Verified 7 - H11 2 O 23 YPL166W ATG29 - Autophagy-specific protein that is required for recruitment of other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved membrane invagination, response to starvation molecular_function cytoplasm 12086 ORF|Verified 8 - H12 2 O 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 2 YOR128C ADE2 - Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process lyase activity cytoplasm 12384 ORF|Verified 42 - E4 2 P 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 2 P 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 A 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 B 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 B 2 YPL165C SET6 - SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability biological_process molecular_function cellular_component 12087 ORF|Verified 9 - A1 3 B 3 YBR224W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 biological_process molecular_function membrane 13364 - 10 - A2 3 B 4 YPL164C MLH3 - Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination molecular_function nucleus 12088 ORF|Verified 9 - A2 3 B 5 YBR225W - - Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components biological_process molecular_function cellular_component 13365 ORF|Uncharacterized 10 - A3 3 B 6 YPL163C SVS1 - Cell wall and vacuolar protein, required for wild-type resistance to vanadate response to chemical stimulus molecular_function cell wall, vacuole, cytoplasm 12089 ORF|Verified 9 - A3 3 B 7 YBR226C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W biological_process molecular_function cellular_component 13366 - 10 - A4 3 B 8 YPL162C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology biological_process molecular_function membrane, vacuole, cytoplasm 12090 ORF|Uncharacterized 9 - A4 3 B 9 YBR227C MCX1 - Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein folding molecular_function mitochondrion, cytoplasm 13367 ORF|Verified 10 - A5 3 B 10 YPL161C BEM4 ROM7 Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length cytoskeleton organization, signaling molecular_function nucleus, cytoplasm 12091 ORF|Verified 9 - A5 3 B 11 YBR228W SLX1 - Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA replication nuclease activity nucleus, chromosome 13368 ORF|Verified 10 - A6 3 B 12 YPL159C PET20 - Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome generation of precursor metabolites and energy, cellular respiration molecular_function mitochondrion, cytoplasm 12093 ORF|Verified 9 - A6 3 B 13 YBR229C ROT2 GLS2 Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds mitochondrion, endoplasmic reticulum, cytoplasm 13369 ORF|Verified 10 - A7 3 B 14 YPL157W TGS1 - Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs meiotic cell cycle, telomere organization, DNA replication, regulation of organelle organization, regulation of DNA metabolic process, RNA modification, nucleus organization methyltransferase activity nucleus, nucleolus 12095 ORF|Verified 9 - A7 3 B 15 YBR230C OM14 - Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 13370 ORF|Verified 10 - A8 3 B 16 YPL156C PRM4 - Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function membrane 12096 ORF|Verified 9 - A8 3 B 17 YBR231C SWC5 AOR1 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin organization molecular_function nucleus, chromosome 13371 ORF|Verified 10 - A9 3 B 18 YBR222C PCS60 FAT2 Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase biological_process RNA binding, mRNA binding peroxisome, membrane, cytoplasm 13362 ORF|Verified 9 - H12 3 B 19 YDR123C INO2 SCS1|DIE1 Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 14057 ORF|Verified 10 - H1 3 B 20 YPL155C KIP2 - Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization other cytoskeleton, mitochondrion, cytoplasm 12097 ORF|Verified 9 - A10 3 B 21 YBR233W PBP2 HEK1 RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length telomere organization RNA binding, mRNA binding cytoplasm 13373 ORF|Verified 10 - A11 3 B 22 YPL154C PEP4 yscA|PRA1|PHO9 Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates membrane invagination, response to starvation, proteolysis involved in cellular protein catabolic process peptidase activity vacuole, mitochondrion, cytoplasm 12098 ORF|Verified 9 - A11 3 B 23 YBR235W - - Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene transmembrane transport transmembrane transporter activity membrane 13375 ORF|Uncharacterized 10 - A12 3 B 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 C 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 C 2 YDR340W - - Putative protein of unknown function biological_process molecular_function cellular_component 14179 - 11 - B1 3 C 3 YEL043W - - Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold biological_process molecular_function ribosome, endoplasmic reticulum, cytoplasm 10284 ORF|Verified 12 - B2 3 C 4 YDR344C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14181 - 11 - B2 3 C 5 YER028C MIG3 - Probable transcriptional repressor involved in response to toxic agents such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes response to DNA damage stimulus, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 10160 ORF|Verified 12 - B3 3 C 6 YDR345C HXT3 - Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 14182 ORF|Verified 11 - B3 3 C 7 YEL045C - - Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress biological_process molecular_function membrane 10286 - 12 - B4 3 C 8 YDR346C SVF1 SGI1 Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance response to oxidative stress, response to chemical stimulus molecular_function nucleus, cytoplasm 14183 ORF|Verified 11 - B4 3 C 9 YEL046C GLY1 - Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis cellular amino acid metabolic process lyase activity cytoplasm 10287 ORF|Verified 12 - B5 3 C 10 YDR348C PAL1 - Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein biological_process molecular_function cellular bud, cell cortex, site of polarized growth, cytoplasm 14185 ORF|Uncharacterized 11 - B5 3 C 11 YEL047C - FRDS1 Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies response to chemical stimulus oxidoreductase activity membrane fraction, ribosome, mitochondrion, cytoplasm 10288 ORF|Verified 12 - B6 3 C 12 YDR349C YPS7 - Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum cell wall organization or biogenesis peptidase activity endoplasmic reticulum, cytoplasm 14186 ORF|Verified 11 - B6 3 C 13 YEL048C TCA17 - Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Golgi vesicle transport, endosome transport, protein complex biogenesis molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 10289 ORF|Uncharacterized 12 - B7 3 C 14 YDR351W SBE2 - Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth cell wall organization or biogenesis molecular_function cytoplasm, Golgi apparatus 14188 ORF|Verified 11 - B7 3 C 15 YEL049W PAU2 - Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component 10290 ORF|Verified 12 - B8 3 C 16 YDR352W - - Putative protein of unknown function biological_process molecular_function membrane, vacuole, cytoplasm 14189 ORF|Uncharacterized 11 - B8 3 C 17 YEL050C RML2 - Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor cytoplasmic translation, lipid metabolic process structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 10291 ORF|Verified 12 - B9 3 C 18 YDR354W TRP4 - Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis cellular amino acid metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm 14191 ORF|Verified 11 - B9 3 C 19 YEL051W VMA8 - Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 10292 ORF|Verified 12 - B10 3 C 20 YDR357C CNL1 BLC1 Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 14194 ORF|Uncharacterized 11 - B10 3 C 21 YEL052W AFG1 - Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain protein targeting, proteolysis involved in cellular protein catabolic process, peroxisome organization, response to oxidative stress, response to chemical stimulus molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 10293 ORF|Verified 12 - B11 3 C 22 YDR358W GGA1 - Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Golgi vesicle transport, protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process small conjugating protein binding cytoplasm, Golgi apparatus 14195 ORF|Verified 11 - B11 3 C 23 YEL053C MAK10 NAA35 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible protein acylation transferase activity, transferring acyl groups cytoplasm 10294 ORF|Verified 12 - B12 3 C 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 D 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 D 2 YPL150W - - Putative protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study biological_process kinase activity cellular_component 12102 ORF|Uncharacterized 9 - B1 3 D 3 YBR239C ERT1 - Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition biological_process DNA binding nucleus, cytoplasm 13379 ORF|Uncharacterized 10 - B2 3 D 4 YPL149W ATG5 APG5 Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation protein targeting, membrane invagination, response to starvation, protein lipidation ligase activity vacuole, cytoplasm 12103 ORF|Verified 9 - B2 3 D 5 YBR240C THI2 phoF|PHO6 Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type vitamin metabolic process, transcription from RNA polymerase II promoter protein binding transcription factor activity, transcription factor binding nucleus 13380 ORF|Verified 10 - B3 3 D 6 YPL147W PXA1 SSH2|PAT2|PAL1|LPI1 Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins lipid transport ATPase activity, transmembrane transporter activity peroxisome, membrane, cytoplasm 12105 ORF|Verified 9 - B3 3 D 7 YBR241C - - Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm 13381 ORF|Uncharacterized 10 - B4 3 D 8 YPL145C KES1 BSR3|OSH4|LPI3 Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex Golgi vesicle transport, lipid transport, membrane invagination, exocytosis, endocytosis lipid binding endomembrane system, membrane, Golgi apparatus, cytoplasm 12107 ORF|Verified 9 - B4 3 D 9 YBR242W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene biological_process molecular_function nucleus, cytoplasm 13382 ORF|Uncharacterized 10 - B5 3 D 10 YPL144W POC4 DMP1|PBA4 Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions protein complex biogenesis molecular_function cytoplasm 12108 ORF|Verified 9 - B5 3 D 11 YBR244W GPX2 AMI1 Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 13384 ORF|Verified 10 - B6 3 D 12 YPL141C FRK1 - Putative protein kinase; similar to Kin4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3 biological_process kinase activity cytoplasm 12111 ORF|Uncharacterized 9 - B6 3 D 13 YBR245C ISW1 SGN2 Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation transcription from RNA polymerase I promoter, chromatin organization, protein complex biogenesis, transcription initiation, DNA-dependent, transcription termination, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter DNA binding, ATPase activity nucleus, chromosome, nucleolus 13385 ORF|Verified 10 - B7 3 D 14 YPL140C MKK2 SSP33|LPI6 Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p protein phosphorylation, peroxisome organization, signaling kinase activity other 12112 ORF|Verified 9 - B7 3 D 15 YBR246W RRT2 ERE1 Diphthamide synthetase; required for last step of diphthamide biosynthesis, deletion leads to accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription peptidyl-amino acid modification, endosome transport ligase activity cytoplasm 13386 ORF|Uncharacterized 10 - B8 3 D 16 YPL139C UME1 WTM3 Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p transcription from RNA polymerase I promoter, response to heat, meiotic cell cycle, transcription from RNA polymerase II promoter, regulation of cell cycle protein binding transcription factor activity nucleus, chromosome 12113 ORF|Verified 9 - B8 3 D 17 YBR248C HIS7 - Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, transferring glycosyl groups other 13388 ORF|Verified 10 - B9 3 D 18 YPL138C SPP1 SAF41|CPS40 Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein protein alkylation, chromatin organization, histone modification histone binding, methyltransferase activity nucleus 12114 ORF|Verified 9 - B9 3 D 19 YBR249C ARO4 - 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups nucleus, cytoplasm 13389 ORF|Verified 10 - B10 3 D 20 YPL136W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C biological_process molecular_function cellular_component 12115 - 9 - B10 3 D 21 YBR250W SPO23 - Protein of unknown function; associates with meiosis-specific protein Spo1p meiotic cell cycle molecular_function cellular_component 13390 ORF|Verified 10 - B11 3 D 22 YPL135W ISU1 NUA1 Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable cofactor metabolic process, cellular ion homeostasis, tRNA processing, RNA modification ion binding mitochondrion, cytoplasm 12117 ORF|Verified 9 - B11 3 D 23 YBR255W MTC4 - Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm 13395 ORF|Uncharacterized 10 - B12 3 D 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 E 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 E 2 YDR360W OPI7 - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. biological_process molecular_function cellular_component 14197 - 11 - C1 3 E 3 YEL056W HAT2 - Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification histone binding, transferase activity, transferring acyl groups nucleus, cytoplasm 10297 ORF|Verified 12 - C2 3 E 4 YDR363W ESC2 - Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member mitotic cell cycle, DNA repair, organelle fission, chromosome segregation, response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus 14200 ORF|Verified 11 - C2 3 E 5 YEL057C - - Protein of unknown function involved in telomere maintenance; target of UME6 regulation biological_process molecular_function cellular_component 10298 ORF|Uncharacterized 12 - C3 3 E 6 YDR364C CDC40 SLU4|SLT15|PRP17 Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats mitotic cell cycle, RNA splicing, mRNA processing other nucleus 14201 ORF|Verified 11 - C3 3 E 7 YEL059W HHY1 ENV6 Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking biological_process molecular_function membrane 10301 - 12 - C4 3 E 8 YDR368W YPR1 - NADPH-dependent aldo-keto reductase, reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 14204 ORF|Verified 11 - C4 3 E 9 YEL060C PRB1 CVT1 Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p sporulation, response to starvation peptidase activity vacuole, cytoplasm 10302 ORF|Verified 12 - C5 3 E 10 YDR369C XRS2 - Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling meiotic cell cycle, DNA repair, telomere organization, sporulation, response to DNA damage stimulus, DNA recombination protein binding, bridging, DNA binding nucleus 14205 ORF|Verified 11 - C5 3 E 11 YEL061C CIN8 SDS15|KSL2 Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation other nucleus, cytoskeleton, chromosome, mitochondrion, cytoplasm 10303 ORF|Verified 12 - C6 3 E 12 YDR370C - - Putative protein of unknown function biological_process molecular_function cytoplasm 14206 ORF|Uncharacterized 11 - C6 3 E 13 YEL062W NPR2 - Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline response to chemical stimulus, amino acid transport, response to starvation, signaling molecular_function vacuole, membrane, cytoplasm 10304 ORF|Verified 12 - C7 3 E 14 YDR371W CTS2 - Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect sporulation molecular_function cytoplasm 14207 ORF|Uncharacterized 11 - C7 3 E 15 YEL063C CAN1 - Plasma membrane arginine permease, requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance transmembrane transport, amino acid transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 10305 ORF|Verified 12 - C8 3 E 16 YDR372C VPS74 MNN3|API1 Protein required for Golgi localization of glycosyltransferases; binds the cytosolic domains of Golgi glycosyltransferases; binding to PtdIns4P required for Golgi targeting and function; tetramer formation required for function response to chemical stimulus, Golgi vesicle transport, signaling lipid binding, enzyme binding nucleus, membrane, Golgi apparatus, cytoplasm 14208 ORF|Verified 11 - C8 3 E 17 YEL064C AVT2 - Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters other other membrane, endoplasmic reticulum, cytoplasm 10306 ORF|Verified 12 - C9 3 E 18 YDR374C - - Putative protein of unknown function biological_process molecular_function cellular_component 14210 ORF|Uncharacterized 11 - C9 3 E 19 YEL065W SIT1 ARN3 Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, cytoplasm 10307 ORF|Verified 12 - C10 3 E 20 YDR378C LSM6 - Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA RNA splicing, rRNA processing, ribosomal small subunit biogenesis, RNA catabolic process, mRNA processing RNA binding nucleus, nucleolus, cytoplasm 14214 ORF|Verified 11 - C10 3 E 21 YEL066W HPA3 - D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro cellular amino acid metabolic process transferase activity, transferring acyl groups nucleus, cytoplasm 10308 ORF|Verified 12 - C11 3 E 22 YDR379W RGA2 - GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p invasive growth in response to glucose limitation, cell budding, cytoskeleton organization, response to chemical stimulus, conjugation, pseudohyphal growth, signaling enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 14215 ORF|Verified 11 - C11 3 E 23 YEL067C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 10309 ORF|Uncharacterized 12 - C12 3 E 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 F 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 F 2 YPL130W SPO19 - Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation meiotic cell cycle, sporulation molecular_function cell wall, membrane 12122 ORF|Verified 9 - C1 3 F 3 YBR259W - - Putative protein of unknown function; YBR259W is not an essential gene biological_process molecular_function cellular_component 13399 ORF|Uncharacterized 10 - C2 3 F 4 YPL129W TAF14 TafII30|ANC1|TFG3|TAF30|SWP29 Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes, involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription initiation, DNA-dependent, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter protein binding transcription factor activity, nucleic acid binding transcription factor activity nucleus, chromosome 12123 ORF|Verified 9 - C2 3 F 5 YBR260C RGD1 - GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization response to chemical stimulus, signaling, response to osmotic stress lipid binding, enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 13400 ORF|Verified 10 - C3 3 F 6 YPL127C HHO1 - Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation DNA recombination, regulation of DNA metabolic process DNA binding nucleus, chromosome 12125 ORF|Verified 9 - C3 3 F 7 YBR261C TAE1 NTM1 AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm peptidyl-amino acid modification, cytoplasmic translation, protein alkylation methyltransferase activity cytoplasm 13401 ORF|Uncharacterized 10 - C4 3 F 8 YPL125W KAP120 LPH2 Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p protein targeting, nuclear transport protein transporter activity, structural molecule activity nucleus, endomembrane system, membrane, cytoplasm 12127 ORF|Verified 9 - C4 3 F 9 YBR262C AIM5 MCS12|FMP51 Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 13402 ORF|Uncharacterized 10 - C5 3 F 10 YPL123C RNY1 - Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity RNA catabolic process, cell morphogenesis nuclease activity extracellular region, vacuole, cytoplasm 12129 ORF|Verified 9 - C5 3 F 11 YBR263W SHM1 TMP3|SHMT1 Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine cellular amino acid metabolic process other mitochondrion, cytoplasm 13403 ORF|Verified 10 - C6 3 F 12 YPL121C MEI5 LPH6 Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination molecular_function nucleus, chromosome 12131 ORF|Verified 9 - C6 3 F 13 YBR264C YPT10 - Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles other GTPase activity cytoplasm 13404 ORF|Verified 10 - C7 3 F 14 YPL120W VPS30 ATG6|VPT30|APG6 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; ortholog of the higher eukaryotic gene Beclin 1 protein targeting, proteolysis involved in cellular protein catabolic process, peroxisome organization, lipid metabolic process, membrane invagination, endosome transport, response to starvation kinase activity membrane fraction, cytoplasm 12132 ORF|Verified 9 - C7 3 F 15 YBR266C SLM6 - Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase membrane invagination, endocytosis molecular_function cellular_component 13406 - 10 - C8 3 F 16 YPL119C DBP1 LPH8 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs translational initiation helicase activity cytoplasm 12133 ORF|Verified 9 - C8 3 F 17 YBR267W REI1 - Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network mitotic cell cycle, cell budding, ribosomal large subunit biogenesis, nuclear transport DNA binding ribosome, cytoplasm 13407 ORF|Verified 10 - C9 3 F 18 YPL118W MRP51 - Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 12134 ORF|Verified 9 - C9 3 F 19 YDR049W VMS1 - Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 13985 ORF|Uncharacterized 10 - C10 3 F 20 YPL116W HOS3 - Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats mitotic cell cycle, chromatin organization, histone modification, transcription from RNA polymerase II promoter hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cellular bud, site of polarized growth, cytoplasm 12136 ORF|Verified 9 - C10 3 F 21 YDR051C DET1 - Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel lipid transport phosphatase activity nucleus, cytoplasm 13987 ORF|Uncharacterized 10 - C11 3 F 22 YPL115C BEM3 - Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly signaling lipid binding, enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoplasm 12137 ORF|Verified 9 - C11 3 F 23 YDR055W PST1 HPF2 Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 cell wall organization or biogenesis molecular_function cell wall, membrane, plasma membrane 13991 ORF|Verified 10 - C12 3 F 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 G 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 G 2 YDR382W RPP2B YP2beta|P2B|L45|YPA1|RPL45 Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 14218 ORF|Verified 11 - D1 3 G 3 YEL071W DLD3 - D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm other oxidoreductase activity cytoplasm 10313 ORF|Verified 12 - D2 3 G 4 YDR383C NKP1 - Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) biological_process molecular_function chromosome 14219 ORF|Verified 11 - D2 3 G 5 YEL072W RMD6 - Protein required for sporulation biological_process molecular_function cellular_component 10314 ORF|Verified 12 - D3 3 G 6 YDR384C ATO3 - Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 14220 ORF|Verified 11 - D3 3 G 7 YER001W MNN1 - Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family protein glycosylation, carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus 10322 ORF|Verified 12 - D4 3 G 8 YDR385W EFT2 - Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin regulation of translation, translational elongation RNA binding, translation factor activity, nucleic acid binding ribosome, cytoplasm 14221 ORF|Verified 11 - D4 3 G 9 YER002W NOP16 - Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 10323 ORF|Verified 12 - D5 3 G 10 YDR386W MUS81 SLX3 Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination, chromosome segregation nuclease activity nucleus 14222 ORF|Verified 11 - D5 3 G 11 YER004W FMP52 - Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 10325 ORF|Verified 12 - D6 3 G 12 YDR387C - - Putative transporter, member of the sugar porter family; YDR387C is not an essential gene transmembrane transport transmembrane transporter activity membrane 14223 ORF|Uncharacterized 11 - D6 3 G 13 YER005W YND1 YEJ5 Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity carbohydrate metabolic process, protein glycosylation other membrane fraction, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 10326 ORF|Verified 12 - D7 3 G 14 YDR388W RVS167 - Actin-associated protein with roles in endocytosis and exocytosis; interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin membrane invagination, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm 14224 ORF|Verified 11 - D7 3 G 15 YER007C-A TMA20 RBF20 Protein of unknown function that associates with ribosomes and has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1 other RNA binding ribosome, cytoplasm 10328 ORF|Verified 12 - D8 3 G 16 YDR389W SAC7 - GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate cytoskeleton organization, signaling enzyme regulator activity other 14225 ORF|Verified 11 - D8 3 G 17 YER007W PAC2 - Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl protein folding cytoskeletal protein binding cellular_component 10329 ORF|Verified 12 - D9 3 G 18 YDR391C - - Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 14227 ORF|Uncharacterized 11 - D9 3 G 19 YER010C - - Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction biological_process molecular_function cellular_component 10332 ORF|Verified 12 - D10 3 G 20 YDR392W SPT3 - Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters peptidyl-amino acid modification, protein acylation, invasive growth in response to glucose limitation, chromatin organization, sporulation, histone modification, conjugation, pseudohyphal growth, transcription from RNA polymerase II promoter protein binding transcription factor activity nucleus 14228 ORF|Verified 11 - D10 3 G 21 YER011W TIR1 SRP1 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking other structural molecule activity cell wall 10333 ORF|Verified 12 - D11 3 G 22 YDR393W SHE9 MDM33 Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 14229 ORF|Verified 11 - D11 3 G 23 YER019W ISC1 - Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance lipid metabolic process, response to osmotic stress other mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 10150 ORF|Verified 12 - D12 3 G 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 H 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 H 2 YPL113C - - Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene other oxidoreductase activity cellular_component 12139 ORF|Uncharacterized 9 - D1 3 H 3 YDR057W YOS9 - ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family proteolysis involved in cellular protein catabolic process other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 13993 ORF|Verified 10 - D2 3 H 4 YPL112C PEX25 - Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p protein targeting, peroxisome organization molecular_function peroxisome, membrane, cytoplasm 12140 ORF|Verified 9 - D2 3 H 5 YDR059C UBC5 - Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible protein modification by small protein conjugation or removal ligase activity other 13994 ORF|Verified 10 - D3 3 H 6 YPL111W CAR1 cargA|LPH15 Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance cellular amino acid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ion binding, enzyme regulator activity nucleus, site of polarized growth, cytoplasm 12141 ORF|Verified 9 - D3 3 H 7 YDR061W - - Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance biological_process molecular_function mitochondrion, cytoplasm 13996 ORF|Uncharacterized 10 - D4 3 H 8 YPL110C GDE1 - Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes lipid metabolic process other ribosome, cytoplasm 12142 ORF|Verified 9 - D4 3 H 9 YDR063W AIM7 GMF1|GMF|Glia maturation factor Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function nucleus, cell cortex, cytoskeleton, cytoplasm 13998 ORF|Uncharacterized 10 - D5 3 H 10 YPL109C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 12143 ORF|Uncharacterized 9 - D5 3 H 11 YDR065W RRG1 - Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cellular ion homeostasis, mitochondrion organization molecular_function mitochondrion, cytoplasm 14000 ORF|Uncharacterized 10 - D6 3 H 12 YPL108W - - Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cytoplasm 12144 ORF|Uncharacterized 9 - D6 3 H 13 YDR066C RTR2 - Protein of unknown function with high similarity to Rtr1p; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene biological_process molecular_function cytoplasm 14001 ORF|Uncharacterized 10 - D7 3 H 14 YPL107W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 12145 ORF|Uncharacterized 9 - D7 3 H 15 YDR067C OCA6 - Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT biological_process molecular_function cytoplasm 14002 ORF|Uncharacterized 10 - D8 3 H 16 YPL106C SSE1 MSI3|LPG3 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm protein folding enzyme regulator activity cytoplasm 12146 ORF|Verified 9 - D8 3 H 17 YDR068W DOS2 - Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 14003 ORF|Verified 10 - D9 3 H 18 YPL105C SYH1 MYR1 Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan nucleus organization molecular_function membrane, ribosome, mitochondrion, cytoplasm 12147 ORF|Verified 9 - D9 3 H 19 YDR069C DOA4 UBP4|SSV7|NPI2|MUT4|DOS1 Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole DNA replication, proteolysis involved in cellular protein catabolic process, membrane invagination, protein modification by small protein conjugation or removal, regulation of DNA metabolic process, endocytosis peptidase activity membrane fraction, mitochondrion, cytoplasm 14004 ORF|Verified 10 - D10 3 H 20 YPL103C FMP30 - Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D lipid metabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm 12149 ORF|Uncharacterized 9 - D10 3 H 21 YDR070C FMP16 - Putative protein of unknown function; proposed to be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 14005 ORF|Uncharacterized 10 - D11 3 H 22 YPL101W ELP4 KTI9|TOT7|HAP1 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity tRNA processing, transcription from RNA polymerase II promoter, RNA modification molecular_function cytoplasm 12150 ORF|Verified 9 - D11 3 H 23 YDR072C IPT1 KTI6|SYR4 Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid;, can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin lipid metabolic process other membrane, membrane fraction 14007 ORF|Verified 10 - D12 3 H 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 I 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 I 2 YDR399W HPT1 HGPRTase|HPRT|BRA6 Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the formation of both inosine monophosphate and guanosine monophosphate; mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups nucleus, cytoplasm 14235 ORF|Verified 11 - E1 3 I 3 YER020W GPA2 SSP101 Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery sporulation, response to chemical stimulus, pseudohyphal growth, nucleobase-containing small molecule metabolic process, signaling GTPase activity membrane fraction, mitochondrion, membrane, cytoplasm, plasma membrane 10152 ORF|Verified 12 - E2 3 I 4 YDR400W URH1 - Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways cofactor metabolic process, nucleobase-containing small molecule metabolic process, proteolysis involved in cellular protein catabolic process hydrolase activity, acting on glycosyl bonds nucleus, cytoplasm 14236 ORF|Verified 11 - E2 3 I 5 YER024W YAT2 - Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane cellular amino acid metabolic process transferase activity, transferring acyl groups cytoplasm 10156 ORF|Verified 12 - E3 3 I 6 YDR401W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14237 - 11 - E3 3 I 7 YER030W CHZ1 - Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif chromatin organization histone binding nucleus 10162 ORF|Verified 12 - E4 3 I 8 YDR402C DIT2 CYP56 N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s sporulation, cell wall organization or biogenesis oxidoreductase activity cellular_component 14238 ORF|Verified 11 - E4 3 I 9 YER033C ZRG8 - Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency cell wall organization or biogenesis molecular_function cellular bud, site of polarized growth, mitochondrion, cytoplasm 10165 ORF|Verified 12 - E5 3 I 10 YDR403W DIT1 - Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure sporulation, cell wall organization or biogenesis other cellular_component 14239 ORF|Verified 11 - E5 3 I 11 YER034W - - Putative protein of unknown function; non-essential gene; expression induced upon calcium shortage biological_process molecular_function nucleus, cytoplasm 10166 ORF|Uncharacterized 12 - E6 3 I 12 YDR405W MRP20 YmL41|MRPL41 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 14241 ORF|Verified 11 - E6 3 I 13 YER035W EDC2 - RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress RNA catabolic process, regulation of translation RNA binding, mRNA binding nucleus, nucleolus, cytoplasm 10167 ORF|Verified 12 - E7 3 I 14 YDR406W PDR15 - Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element response to chemical stimulus ATPase activity, transmembrane transporter activity membrane 14242 ORF|Verified 11 - E7 3 I 15 YER038W-A - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria biological_process molecular_function mitochondrion, cytoplasm 10171 - 12 - E8 3 I 16 YDR408C ADE8 - Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process other nucleus, cytoplasm 14244 ORF|Verified 11 - E8 3 I 17 YER040W GLN3 - Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 10173 ORF|Verified 12 - E9 3 I 18 YDR409W SIZ1 ULL1 SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring protein modification by small protein conjugation or removal, chromosome segregation ligase activity nucleus, cell cortex, cytoskeleton, chromosome, cytoplasm 14245 ORF|Verified 11 - E9 3 I 19 YER041W YEN1 - Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p response to DNA damage stimulus, DNA repair nuclease activity nucleus, cytoplasm 10174 ORF|Verified 12 - E10 3 I 20 YDR410C STE14 - Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane protein alkylation, protein maturation methyltransferase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 14246 ORF|Verified 11 - E10 3 I 21 YER042W MXR1 MSRA Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 10175 ORF|Verified 12 - E11 3 I 22 YDR411C DFM1 - Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p proteolysis involved in cellular protein catabolic process, response to chemical stimulus, signaling molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm 14247 ORF|Verified 11 - E11 3 I 23 YER044C-A MEI4 - Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic cell cycle molecular_function nucleus, chromosome 10178 ORF|Verified 12 - E12 3 I 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 J 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 J 2 YPL100W ATG21 HSV1|MAI1 Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein protein targeting, vesicle organization, membrane invagination, response to starvation, protein lipidation lipid binding vacuole, membrane, cytoplasm 12152 ORF|Verified 9 - E1 3 J 3 YDR075W PPH3 - Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes meiotic cell cycle, DNA repair, protein dephosphorylation, response to DNA damage stimulus, DNA recombination, signaling, regulation of cell cycle phosphatase activity nucleus, chromosome, cytoplasm 14010 ORF|Verified 10 - E2 3 J 4 YPL099C AIM43 FMP14 Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 12153 ORF|Uncharacterized 9 - E2 3 J 5 YDR076W RAD55 - Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p response to DNA damage stimulus, meiotic cell cycle, DNA repair, DNA recombination ATPase activity nucleus 14011 ORF|Verified 10 - E3 3 J 6 YPL098C MGR2 - Protein required for growth of cells lacking the mitochondrial genome biological_process molecular_function cellular_component 12154 ORF|Verified 9 - E3 3 J 7 YDR078C SHU2 - Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function response to DNA damage stimulus, DNA repair, DNA recombination molecular_function other 14013 ORF|Verified 10 - E4 3 J 8 YPL097W MSY1 - Mitochondrial tyrosyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 12155 ORF|Verified 9 - E4 3 J 9 YDR079W PET100 - Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme mitochondrion organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 14014 ORF|Verified 10 - E5 3 J 10 YPL096W PNG1 - Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p proteolysis involved in cellular protein catabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, mitochondrion, cytoplasm 12156 ORF|Verified 9 - E5 3 J 11 YDR080W VPS41 VPL20|VAM2|SVL2|FET2|CVT8 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport vacuole organization, regulation of organelle organization, regulation of transport, organelle fusion, vesicle organization, protein maturation, protein complex biogenesis, membrane fusion, membrane invagination lipid binding, enzyme regulator activity vacuole, membrane, cytoplasm 14015 ORF|Verified 10 - E6 3 J 12 YPL095C EEB1 - Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification lipid metabolic process transferase activity, transferring acyl groups cellular_component 12157 ORF|Verified 9 - E6 3 J 13 YDR083W RRP8 - Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation rRNA processing methyltransferase activity nucleus, nucleolus 14018 ORF|Verified 10 - E7 3 J 14 YPL092W SSU1 LPG16 Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein ion transport transmembrane transporter activity membrane, plasma membrane 12160 ORF|Verified 9 - E7 3 J 15 YDR084C TVP23 - Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern other molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus 14019 ORF|Verified 10 - E8 3 J 16 YBR173C UMP1 RNS2 Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, cytoplasm 13313 ORF|Verified 9 - E8 3 J 17 YDR085C AFR1 - Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p cell morphogenesis, conjugation, signaling, response to chemical stimulus signal transducer activity other 14020 ORF|Verified 10 - E9 3 J 18 YBR174C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective biological_process molecular_function cellular_component 13314 - 9 - E9 3 J 19 YDR089W - - Protein of unknown function; deletion confers resistance to Nickel other molecular_function membrane 14024 ORF|Uncharacterized 10 - E10 3 J 20 YBR175W SWD3 SAF35|CPS30 Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 telomere organization, protein alkylation, chromatin organization, histone modification methyltransferase activity nucleus 13315 ORF|Verified 9 - E10 3 J 21 YDR090C - - Putative protein of unknown function biological_process molecular_function membrane 14025 ORF|Uncharacterized 10 - E11 3 J 22 YBR176W ECM31 - Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process other mitochondrion, cytoplasm 13316 ORF|Verified 9 - E11 3 J 23 YDR092W UBC13 - Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus response to DNA damage stimulus, DNA repair, protein modification by small protein conjugation or removal, protein complex biogenesis ligase activity nucleus, cytoplasm 14027 ORF|Verified 10 - E12 3 J 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 K 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 K 2 YDR415C - - Putative protein of unknown function biological_process molecular_function cellular_component 14251 ORF|Uncharacterized 11 - F1 3 K 3 YER046W-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10181 - 12 - F2 3 K 4 YDR418W RPL12B YL23|L15B|L12B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 14254 ORF|Verified 11 - F2 3 K 5 YER047C SAP1 - Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system biological_process ATPase activity cytoplasm 10182 ORF|Verified 12 - F3 3 K 6 YDR419W RAD30 DBH1 DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; mutations in human pol eta are responsible for XPV response to DNA damage stimulus, DNA repair nucleotidyltransferase activity chromosome 14255 ORF|Verified 11 - F3 3 K 7 YER048C CAJ1 - Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly protein folding other nucleus 10183 ORF|Verified 12 - F4 3 K 8 YDR420W HKR1 - Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection carbohydrate metabolic process, cytokinesis, cell wall organization or biogenesis, signaling, response to osmotic stress signal transducer activity membrane, site of polarized growth, plasma membrane 14256 ORF|Verified 11 - F4 3 K 9 YER049W TPA1 - Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate RNA catabolic process RNA binding, mRNA binding nucleus 10184 ORF|Verified 12 - F5 3 K 10 YDR421W ARO80 - Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 14257 ORF|Verified 11 - F5 3 K 11 YER051W JHD1 KDM2|JHDM1 JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe chromatin organization, histone modification histone binding, oxidoreductase activity cellular_component 10186 ORF|Verified 12 - F6 3 K 12 YDR422C SIP1 - Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions protein phosphorylation, signaling, protein complex biogenesis kinase activity vacuole, cytoplasm 14258 ORF|Verified 11 - F6 3 K 13 YER053C PIC2 - Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, cytoplasm 10188 ORF|Verified 12 - F7 3 K 14 YDR423C CAD1 YAP2 AP-1-like basic leucine zipper (bZIP) transcriptional activator involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA transcription from RNA polymerase II promoter, response to chemical stimulus nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 14259 ORF|Verified 11 - F7 3 K 15 YER054C GIP2 - Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p carbohydrate metabolic process, protein dephosphorylation, generation of precursor metabolites and energy enzyme regulator activity cytoplasm 10189 ORF|Verified 12 - F8 3 K 16 YDR425W SNX41 - Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p endosome transport lipid binding cytoplasm 14261 ORF|Verified 11 - F8 3 K 17 YER056C FCY2 BRA7 Purine-cytosine permease, mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 10191 ORF|Verified 12 - F9 3 K 18 YDR426C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 biological_process molecular_function cellular_component 14262 - 11 - F9 3 K 19 YER056C-A RPL34A L34A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Bp and has similarity to rat L34 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 10192 ORF|Verified 12 - F10 3 K 20 YDR428C BNA7 - Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component 14264 ORF|Uncharacterized 11 - F10 3 K 21 YER058W PET117 - Protein required for assembly of cytochrome c oxidase mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 10194 ORF|Verified 12 - F11 3 K 22 YDR430C CYM1 MOP112 Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis involved in cellular protein catabolic process peptidase activity mitochondrial envelope, mitochondrion, cytoplasm 14266 ORF|Verified 11 - F11 3 K 23 YER059W PCL6 - Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other 10195 ORF|Verified 12 - F12 3 K 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 L 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 L 2 YBR178W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C biological_process molecular_function cellular_component 13318 - 9 - F1 3 L 3 YDR094W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 biological_process molecular_function cellular_component 14029 - 10 - F2 3 L 4 YBR180W DTR1 - Putative dityrosine transporter, required for spore wall synthesis; expressed during sporulation; member of the major facilitator superfamily (DHA1 family) of multidrug resistance transporters sporulation, transmembrane transport, cell wall organization or biogenesis transmembrane transporter activity membrane 13320 ORF|Verified 9 - F2 3 L 5 YDR095C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14030 - 10 - F3 3 L 6 YBR181C RPS6B RPS102|RPS101|LPG18 Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 13321 ORF|Verified 9 - F3 3 L 7 YDR096W GIS1 - JmjC domain-containing histone demethylase and transcription factor; involved in expression of genes during nutrient limitation; negatively regulates DPP1 and PHR1; activity is modulated by limited proteasome-mediated proteolysis; has a JmjC and a JmjN domain in the N-terminal region that interact, promoting Gis1p stability and proper transcriptional activity; contains transactivating domains TAD1 and TAD2 downstream of the Jmj domains and a C-terminal DNA binding domain chromatin organization, histone modification, lipid metabolic process, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, oxidoreductase activity, DNA binding nucleus, mitochondrion, cytoplasm 14031 ORF|Verified 10 - F4 3 L 8 YBR182C SMP1 - Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 13322 ORF|Verified 9 - F4 3 L 9 YDR097C MSH6 PMS3 Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p response to DNA damage stimulus, meiotic cell cycle, DNA repair DNA binding, ATPase activity nucleus 14032 ORF|Verified 10 - F5 3 L 10 YBR183W YPC1 alkaline ceramidase Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance lipid metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, transferase activity, transferring acyl groups, hydrolase activity, acting on glycosyl bonds membrane, endoplasmic reticulum, cytoplasm 13323 ORF|Verified 9 - F5 3 L 11 YDR098C GRX3 - Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage cellular ion homeostasis, cytoskeleton organization, response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 14033 ORF|Verified 10 - F6 3 L 12 YBR184W - - Putative protein of unknown function; YBR184W is not an essential gene biological_process molecular_function cellular_component 13324 ORF|Uncharacterized 9 - F6 3 L 13 YDR099W BMH2 SCD3 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling mitotic cell cycle, carbohydrate metabolic process, DNA replication, sporulation, generation of precursor metabolites and energy, response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, pseudohyphal growth, cell wall organization or biogenesis, signaling, regulation of cell cycle DNA binding nucleus, membrane fraction 14034 ORF|Verified 10 - F7 3 L 14 YBR185C MBA1 - Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 13325 ORF|Verified 9 - F7 3 L 15 YDR100W TVP15 - Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 14035 ORF|Verified 10 - F8 3 L 16 YBR186W PCH2 - Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA meiotic cell cycle, regulation of cell cycle molecular_function nucleus, nucleolus 13326 ORF|Verified 9 - F8 3 L 17 YDR101C ARX1 - Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex ribosomal large subunit biogenesis molecular_function nucleus, ribosome, cytoplasm 14036 ORF|Verified 10 - F9 3 L 18 YBR187W GDT1 - Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis biological_process molecular_function membrane, vacuole, cytoplasm 13327 ORF|Uncharacterized 9 - F9 3 L 19 YDR102C - - Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index biological_process molecular_function cellular_component 14037 - 10 - F10 3 L 20 YBR188C NTC20 - Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs RNA splicing, mRNA processing other nucleus 13328 ORF|Verified 9 - F10 3 L 21 YDR104C SPO71 - Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis sporulation, cell wall organization or biogenesis molecular_function cell wall 14039 ORF|Verified 10 - F11 3 L 22 YBR194W AIM4 SOY1 Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress biological_process molecular_function cytoplasm 13334 ORF|Uncharacterized 9 - F11 3 L 23 YDR105C TMS1 - Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance biological_process molecular_function membrane, vacuole, cytoplasm 14040 ORF|Verified 10 - F12 3 L 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 M 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 M 2 YDR432W NPL3 NAB1|NOP3|MTS1|MTR13 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm RNA splicing, nucleobase-containing compound transport, transcription termination, DNA-dependent, transcription elongation, DNA-dependent, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport RNA binding, enzyme binding, mRNA binding nucleus, cytoplasm 14268 ORF|Verified 11 - G1 3 M 3 YER062C HOR2 GPP2 One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition carbohydrate metabolic process, generation of precursor metabolites and energy, response to osmotic stress phosphatase activity nucleus, cytoplasm 10199 ORF|Verified 12 - G2 3 M 4 YDR433W - KRE22 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data response to DNA damage stimulus molecular_function cellular_component 14269 - 11 - G2 3 M 5 YER065C ICL1 - Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose carbohydrate metabolic process lyase activity cellular_component 10202 ORF|Verified 12 - G3 3 M 6 YDR435C PPM1 - Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits protein alkylation, protein complex biogenesis methyltransferase activity cellular_component 14271 ORF|Verified 11 - G3 3 M 7 YER067W RGI1 - Protein of unknown function involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion generation of precursor metabolites and energy molecular_function nucleus, membrane fraction, cytoplasm 10205 ORF|Uncharacterized 12 - G4 3 M 8 YDR436W PPZ2 - Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis phosphatase activity cellular_component 14272 ORF|Verified 11 - G4 3 M 9 YER067C-A - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W biological_process molecular_function cellular_component 10206 - 12 - G5 3 M 10 YEL001C IRC22 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endoplasmic reticulum, cytoplasm 10241 ORF|Uncharacterized 11 - G5 3 M 11 YER044C ERG28 BUD18 Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p lipid metabolic process protein binding, bridging membrane, endomembrane system, endoplasmic reticulum, cytoplasm 10177 ORF|Verified 12 - G6 3 M 12 YEL003W GIM4 PFD2 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it protein complex biogenesis cytoskeletal protein binding cytoplasm 10243 ORF|Verified 11 - G6 3 M 13 YER068C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10208 - 12 - G7 3 M 14 YEL005C VAB2 VAB31 Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter biological_process molecular_function cytoplasm 10245 ORF|Verified 11 - G7 3 M 15 YER069W ARG5,6 - Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p cellular amino acid metabolic process kinase activity, oxidoreductase activity mitochondrion, cytoplasm 10209 ORF|Verified 12 - G8 3 M 16 YEL006W YEA6 NDT2 Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog other other membrane, mitochondrial envelope, mitochondrion, cytoplasm 10246 ORF|Verified 11 - G8 3 M 17 YER071C TDA2 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function site of polarized growth, cytoplasm 10211 ORF|Uncharacterized 12 - G9 3 M 18 YEL007W MIT1 TOS9 Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 biological_process molecular_function nucleus, cytoplasm 10247 ORF|Uncharacterized 11 - G9 3 M 19 YER072W VTC1 PHM4|NRF1 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity vacuole organization, membrane fusion, membrane invagination RNA binding, mRNA binding vacuole, membrane, endoplasmic reticulum, cytoplasm 10212 ORF|Verified 12 - G10 3 M 20 YEL008W - - Hypothetical protein predicted to be involved in metabolism biological_process molecular_function cellular_component 10248 - 11 - G10 3 M 21 YER073W ALD5 - Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed other oxidoreductase activity mitochondrion, cytoplasm 10213 ORF|Verified 12 - G11 3 M 22 YEL009C GCN4 AAS101|ARG9|AAS3 Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels transcription initiation, DNA-dependent, transcription from RNA polymerase II promoter, response to chemical stimulus, protein complex biogenesis protein binding transcription factor activity, nucleic acid binding transcription factor activity, DNA binding nucleus 10249 ORF|Verified 11 - G11 3 M 23 YER074W RPS24A S24A|RPS24EA Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 10214 ORF|Verified 12 - G12 3 M 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 N 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 N 2 YBR197C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene biological_process molecular_function nucleus, cytoplasm 13337 ORF|Uncharacterized 9 - G1 3 N 3 YDR108W TRS85 GSG1|MUM1 Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role meiotic cell cycle, protein targeting, vesicle organization, peroxisome organization, Golgi vesicle transport, membrane invagination, response to starvation molecular_function Golgi apparatus, cytoplasm 14042 ORF|Verified 10 - G2 3 N 4 YBR199W KTR4 - Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups cytoplasm, Golgi apparatus 13339 ORF|Verified 9 - G2 3 N 5 YDR109C - - Putative kinase biological_process molecular_function cellular_component 14043 ORF|Uncharacterized 10 - G3 3 N 6 YBR200W BEM1 SRO1 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p cell morphogenesis, conjugation, response to chemical stimulus structural molecule activity, lipid binding cellular bud, site of polarized growth 13340 ORF|Verified 9 - G3 3 N 7 YDR110W FOB1 HRM1 Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases mitotic cell cycle, organelle fission, chromatin organization, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process DNA binding nucleus, nucleolus 14044 ORF|Verified 10 - G4 3 N 8 YBR201W DER1 - Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 13341 ORF|Verified 9 - G4 3 N 9 YDR111C ALT2 - Putative alanine transaminase (glutamic pyruvic transaminase) biological_process other nucleus, cytoplasm 14045 ORF|Uncharacterized 10 - G5 3 N 10 YBR203W COS111 - Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signaling molecular_function mitochondrion, cytoplasm 13343 ORF|Verified 9 - G5 3 N 11 YDR112W IRC2 - Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component 14046 - 10 - G6 3 N 12 YBR204C LDH1 - Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) other other cytoplasm 13344 ORF|Uncharacterized 9 - G6 3 N 13 YDR116C MRPL1 - Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 14050 ORF|Verified 10 - G7 3 N 14 YBR205W KTR3 - Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles protein glycosylation, carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups vacuole, cytoplasm 13345 ORF|Verified 9 - G7 3 N 15 YDR117C TMA64 RBF64 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity biological_process RNA binding ribosome, cytoplasm 14051 ORF|Verified 10 - G8 3 N 16 YBR206W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 biological_process molecular_function cellular_component 13346 - 9 - G8 3 N 17 YDR119W VBA4 - Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function membrane, vacuole, cytoplasm 14053 ORF|Uncharacterized 10 - G9 3 N 18 YBR207W FTH1 - Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis transmembrane transport, ion transport, membrane invagination, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm 13347 ORF|Verified 9 - G9 3 N 19 YDR120C TRM1 - tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments tRNA processing, RNA modification methyltransferase activity nucleus, membrane, endomembrane system, mitochondrion, cytoplasm 14054 ORF|Verified 10 - G10 3 N 20 YBR208C DUR1,2 DUR80 Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, ligase activity cytoplasm 13348 ORF|Verified 9 - G10 3 N 21 YDR121W DPB4 - Shared subunit of DNA polymerase (II) epsilon and of ISW2/yCHRAC chromatin accessibility complex; involved in both chromosomal DNA replication and in inheritance of telomeric silencing DNA replication DNA binding, nucleotidyltransferase activity nucleus, chromosome 14055 ORF|Verified 10 - G11 3 N 22 YBR209W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene biological_process molecular_function cellular_component 13349 - 9 - G11 3 N 23 YDR122W KIN1 - Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p protein phosphorylation, exocytosis kinase activity membrane, plasma membrane 14056 ORF|Verified 10 - G12 3 N 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 O 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 O 2 YEL013W VAC8 YEB3 Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions vacuole organization, protein targeting, organelle assembly, vesicle organization, membrane fusion, membrane invagination, organelle inheritance, response to starvation other vacuole, membrane, cytoplasm 10253 ORF|Verified 11 - H1 3 O 3 YER080W AIM9 FMP29 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 10220 ORF|Uncharacterized 12 - H2 3 O 4 YEL014C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10254 - 11 - H2 3 O 5 YEL027W VMA3 CUP5|GEF2|CLS7 Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis vacuole organization, protein targeting, ion transport, cellular ion homeostasis, membrane invagination, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm 10268 ORF|Verified 12 - A1 3 O 6 YDR338C - - Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily transmembrane transport transmembrane transporter activity membrane 14177 ORF|Uncharacterized 11 - A12 3 O 7 YER081W SER3 - 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser33p cellular amino acid metabolic process oxidoreductase activity cytoplasm 10221 ORF|Verified 12 - H4 3 O 8 YEL015W EDC3 LSM16|DCP3 Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies RNA catabolic process molecular_function cytoplasm 10255 ORF|Verified 11 - H4 3 O 9 YER083C GET2 RMD7|HUR2 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Golgi vesicle transport other membrane, endoplasmic reticulum, cytoplasm 10223 ORF|Verified 12 - H5 3 O 10 YEL016C NPP2 - Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation nucleobase-containing small molecule metabolic process nuclease activity cellular_component 10256 ORF|Verified 11 - H5 3 O 11 YER084W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10224 - 12 - H6 3 O 12 YEL017C-A PMP2 - Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; nearly identical to PMP1 ion transport molecular_function membrane 10257 ORF|Verified 11 - H6 3 O 13 YER085C - - Putative protein of unknown function biological_process molecular_function cellular_component 10225 ORF|Uncharacterized 12 - H7 3 O 14 YEL017W GTT3 - Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery biological_process molecular_function cellular_component 10258 ORF|Verified 11 - H7 3 O 15 YER086W ILV1 ISO1 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 10226 ORF|Verified 12 - H8 3 O 16 YEL018W EAF5 - Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex response to DNA damage stimulus, DNA repair molecular_function nucleus 10259 ORF|Verified 11 - H8 3 O 17 YER087W AIM10 - Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process ligase activity mitochondrion, cytoplasm 10227 ORF|Uncharacterized 12 - H9 3 O 18 YEL020C - - Hypothetical protein with low sequence identity to Pdc1p biological_process molecular_function cytoplasm 10261 ORF|Uncharacterized 11 - H9 3 O 19 YER087C-B SBH1 YER087C-A|SEB1 Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p protein targeting, transmembrane transport protein transporter activity, enzyme regulator activity, transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10228 ORF|Verified 12 - H10 3 O 20 YEL023C - - Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene biological_process molecular_function cellular_component 10264 ORF|Uncharacterized 11 - H10 3 O 21 YGR123C PPT1 - Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth; computational analyses suggest roles in phosphate metabolism and rRNA processing protein dephosphorylation phosphatase activity nucleus, cytoplasm 14753 ORF|Verified 12 - H11 3 O 22 YEL024W RIP1 - Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 10265 ORF|Verified 11 - H11 3 O 23 YGR124W ASN2 - Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway cellular amino acid metabolic process ligase activity cytoplasm 14754 ORF|Verified 12 - H12 3 O 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 3 YOR128C ADE2 - Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process lyase activity cytoplasm 12384 ORF|Verified 42 - E4 3 P 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 3 P 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 A 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 B 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 B 2 YGR125W - - Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function membrane, vacuole, cytoplasm 14755 ORF|Uncharacterized 13 - A1 4 B 3 YHR015W MIP6 - Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export nucleobase-containing compound transport, nuclear transport RNA binding nucleus, membrane, endomembrane system 10978 ORF|Verified 14 - A2 4 B 4 YGR126W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm 14756 ORF|Uncharacterized 13 - A2 4 B 5 YHR018C ARG4 - Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway cellular amino acid metabolic process lyase activity cytoplasm 10981 ORF|Verified 14 - A3 4 B 6 YGR127W - - Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component 14757 ORF|Uncharacterized 13 - A3 4 B 7 YHR021C RPS27B rp61|YS20|S27B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Ap and has similarity to rat S27 ribosomal protein cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 10984 ORF|Verified 14 - A4 4 B 8 YGR129W SYF2 NTC31 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest RNA splicing, mRNA processing other nucleus 14759 ORF|Verified 13 - A4 4 B 9 YHR022C - - Putative protein of unknown function; YHR022C is not an essential gene biological_process molecular_function cellular_component 10985 ORF|Uncharacterized 14 - A5 4 B 10 YGR130C - - Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) biological_process molecular_function membrane, cell cortex, cytoplasm 14760 ORF|Uncharacterized 13 - A5 4 B 11 YHR028C DAP2 DPP2 Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p protein maturation peptidase activity membrane, vacuole, cytoplasm 10991 ORF|Verified 14 - A6 4 B 12 YGR131W FHN1 - Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain other molecular_function membrane 14761 ORF|Uncharacterized 13 - A6 4 B 13 YHR029C YHI9 - Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production signaling, response to chemical stimulus molecular_function cellular_component 10992 ORF|Verified 14 - A7 4 B 14 YGR132C PHB1 - Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation protein folding, organelle inheritance, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 14762 ORF|Verified 13 - A7 4 B 15 YHR030C SLT2 SLK2|MPK1|BYC2 Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway transmembrane transport, regulation of transport, peroxisome organization, response to chemical stimulus, mRNA processing, cell wall organization or biogenesis, protein targeting, protein phosphorylation, cytokinesis, nuclear transport, signaling signal transducer activity, kinase activity nucleus, cellular bud, site of polarized growth, cytoplasm 10993 ORF|Verified 14 - A8 4 B 16 YGR133W PEX4 UBC10|PAS2 Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis protein targeting, protein modification by small protein conjugation or removal, peroxisome organization, signaling ligase activity peroxisome, cytoplasm 14763 ORF|Verified 13 - A8 4 B 17 YHR031C RRM3 RTT104 DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p DNA replication, mitochondrion organization, regulation of DNA metabolic process helicase activity, ATPase activity nucleus, chromosome 10994 ORF|Verified 14 - A9 4 B 18 YGR135W PRE9 - Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, endomembrane system, mitochondrion, membrane, cytoplasm 14765 ORF|Verified 13 - A9 4 B 19 YHR033W - - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm 10996 ORF|Uncharacterized 14 - A10 4 B 20 YGR136W LSB1 - Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization biological_process molecular_function nucleus, cytoplasm 14766 ORF|Verified 13 - A10 4 B 21 YHR034C PIH1 NOP17 Protein of unresolved function; may function in protein folding and/or rRNA processing, interacts with a chaperone (Hsp82p), two chromatin remodeling factors (Rvb1p, Rvb2p) and two rRNA processing factors (Rrp43p, Nop58p) protein folding, rRNA processing molecular_function nucleus, cytoplasm 10997 ORF|Verified 14 - A11 4 B 22 YGR137W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14767 - 13 - A11 4 B 23 YHR035W - - Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component 10998 ORF|Uncharacterized 14 - A12 4 B 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 C 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 C 2 YHR210C - - Putative protein of unknown function; non-essential gene; highly expressed under anaeorbic conditions; sequence similarity to aldose 1-epimerases such as GAL10 biological_process molecular_function cellular_component 12904 ORF|Uncharacterized 15 - B1 4 C 3 YLR181C VTA1 - Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain endosome transport, protein complex biogenesis enzyme regulator activity membrane, membrane fraction, cytoplasm 14130 ORF|Verified 16 - B2 4 C 4 YCL001W RER1 - Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm, Golgi apparatus 13409 ORF|Verified 15 - B2 4 C 5 YLR182W SWI6 SDS11|PSL8 Transcription cofactor, forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization mitotic cell cycle, meiotic cell cycle, response to chemical stimulus, mRNA processing, transcription from RNA polymerase II promoter, signaling protein binding transcription factor activity nucleus, cytoplasm 14131 ORF|Verified 16 - B3 4 C 6 YHR209W CRG1 - S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin other RNA binding, methyltransferase activity, mRNA binding cellular_component 12903 ORF|Uncharacterized 15 - A12 4 C 7 YLR165C PUS5 - Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability rRNA processing, RNA modification lyase activity mitochondrion, cytoplasm 14114 ORF|Verified 16 - A1 4 C 8 YCL002C - - Putative protein of unknown function; YCL002C is not an essential gene biological_process molecular_function membrane 13410 ORF|Uncharacterized 15 - B4 4 C 9 YLR183C TOS4 - Forkhead Associated domain containing protein and putative transcription factor found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; similar to PLM2 response to DNA damage stimulus other nucleus, chromosome, mitochondrion, cytoplasm 14132 ORF|Verified 16 - B5 4 C 10 YCL004W PGS1 YCL003W|PEL1 Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis lipid metabolic process other mitochondrion, cytoplasm 13411 ORF|Verified 15 - B5 4 C 11 YLR184W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14133 - 16 - B6 4 C 12 YCL005W LDB16 - Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria biological_process molecular_function mitochondrion, cytoplasm 13413 ORF|Verified 15 - B6 4 C 13 YLR185W RPL37A YL35|L43|L37A Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Bp and to rat L37 ribosomal protein cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 14134 ORF|Verified 16 - B7 4 C 14 YCL007C - CWH36 Dubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A biological_process molecular_function cellular_component 13415 - 15 - B7 4 C 15 YLR187W SKG3 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p biological_process molecular_function cellular bud, site of polarized growth, cytoplasm 14136 ORF|Uncharacterized 16 - B8 4 C 16 YCL008C STP22 VPL15|VPS23 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype protein targeting, endosome transport, proteolysis involved in cellular protein catabolic process small conjugating protein binding membrane, plasma membrane, cytoplasm 13416 ORF|Verified 15 - B8 4 C 17 YLR188W MDL1 - Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress transmembrane transport ATPase activity, transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 14137 ORF|Verified 16 - B9 4 C 18 YCL009C ILV6 - Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria cellular amino acid metabolic process enzyme regulator activity mitochondrion, cytoplasm 13417 ORF|Verified 15 - B9 4 C 19 YLR189C ATG26 UGT51 UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy lipid metabolic process, sporulation transferase activity, transferring glycosyl groups cytoplasm 14138 ORF|Verified 16 - B10 4 C 20 YCL011C GBP2 RLF6 Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro telomere organization, nucleobase-containing compound transport, nuclear transport RNA binding, DNA binding nucleus, cytoplasm 13419 ORF|Verified 15 - B10 4 C 21 YLR190W MMR1 - Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p mitochondrion organization, organelle inheritance molecular_function mitochondrion, cellular bud, membrane, site of polarized growth, mitochondrial envelope, cytoplasm 14139 ORF|Verified 16 - B11 4 C 22 YCL014W BUD3 YCL012W Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding cell budding, cytokinesis molecular_function cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm 13420 ORF|Verified 15 - C1 4 C 23 YLR191W PEX13 PAS20 Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p protein targeting, peroxisome organization, protein complex biogenesis protein binding, bridging peroxisome, membrane, cytoplasm 14140 ORF|Verified 16 - B12 4 C 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 D 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 D 2 YHR013C ARD1 NAA10 Subunit of N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, transferring acyl groups ribosome, cytoplasm 10976 ORF|Verified 13 - H12 4 D 3 YHR157W REC104 - Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiotic cell cycle, DNA recombination molecular_function nucleus, chromosome 12851 ORF|Verified 14 - H1 4 D 4 YGR139W - - Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14769 - 13 - B2 4 D 5 YHR038W RRF1 FIL1|KIM4 Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria mitochondrial translation, mitochondrion organization RNA binding, translation factor activity, nucleic acid binding mitochondrion, cytoplasm 11001 ORF|Verified 14 - B3 4 D 6 YGR141W VPS62 - Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins protein targeting molecular_function cellular_component 14771 ORF|Verified 13 - B3 4 D 7 YHR039C MSC7 - Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids meiotic cell cycle, DNA recombination molecular_function membrane, endoplasmic reticulum, cytoplasm 11002 ORF|Verified 14 - B4 4 D 8 YGR142W BTN2 - v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase endosome transport, amino acid transport other cytoplasm 14772 ORF|Verified 13 - B4 4 D 9 YHR043C DOG2 2-deoxyglucose-6-phosphate phosphatase 2-deoxyglucose-6-phosphate phosphatase, member of a family of low molecular weight phosphatases, similar to Dog1p, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process phosphatase activity nucleus, cytoplasm 11006 ORF|Verified 14 - B5 4 D 10 YGR143W SKN1 - Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p carbohydrate metabolic process, lipid metabolic process, cell wall organization or biogenesis hydrolase activity, acting on glycosyl bonds membrane 14773 ORF|Verified 13 - B5 4 D 11 YHR044C DOG1 2-deoxyglucose-6-phosphate phosphatase 2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p, member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified carbohydrate metabolic process phosphatase activity cellular_component 11007 ORF|Verified 14 - B6 4 D 12 YGR144W THI4 MOL1|ESP35 Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents mitochondrion organization, vitamin metabolic process ion binding cytoplasm 14774 ORF|Verified 13 - B6 4 D 13 YHR046C INM1 - Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate carbohydrate metabolic process phosphatase activity nucleus, cytoplasm 11873 ORF|Verified 14 - B7 4 D 14 YGR146C ECL1 - Protein of unknown function, affects chronological lifespan; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function cellular_component 14776 ORF|Uncharacterized 13 - B7 4 D 15 YHR047C AAP1 AAP1' Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation carbohydrate metabolic process, generation of precursor metabolites and energy peptidase activity nucleus, cytoplasm 11874 ORF|Verified 14 - B8 4 D 16 YGR148C RPL24B rp29|YL21|L30B|L24B|RPL30B Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 14778 ORF|Verified 13 - B8 4 D 17 YHR048W YHK8 - Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles response to chemical stimulus molecular_function membrane 11875 ORF|Uncharacterized 14 - B9 4 D 18 YGR149W - - Putative protein of unknown function; predicted to be an integal membrane protein biological_process molecular_function membrane 14779 ORF|Uncharacterized 13 - B9 4 D 19 YHR049W FSH1 - Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process other nucleus, cytoplasm 11876 ORF|Verified 14 - B10 4 D 20 YGR151C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C biological_process molecular_function cellular_component 14781 - 13 - B10 4 D 21 YHR049C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11877 - 14 - B11 4 D 22 YGR152C RSR1 BUD1 GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases cell budding, cytokinesis, signaling GTPase activity, signal transducer activity cellular bud, membrane, site of polarized growth, plasma membrane 14782 ORF|Verified 13 - B11 4 D 23 YHR050W SMF2 - Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins cellular ion homeostasis, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, mitochondrion, cytoplasm 11878 ORF|Verified 14 - B12 4 D 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 E 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 E 2 YCL014W BUD3 YCL012W Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding cell budding, cytokinesis molecular_function cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm 13422 ORF|Verified 15 - C1 4 E 3 YLR194C - - Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress cell wall organization or biogenesis structural molecule activity cell wall, membrane, plasma membrane 14143 ORF|Uncharacterized 16 - C2 4 E 4 YCL016C DCC1 - Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance response to DNA damage stimulus, mitotic cell cycle, organelle fission, chromosome segregation molecular_function chromosome 13423 ORF|Verified 15 - C2 4 E 5 YLR199C PBA1 POC1 Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly protein complex biogenesis molecular_function cytoplasm 14148 ORF|Verified 16 - C3 4 E 6 YCL024W KCC4 - Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p protein phosphorylation, cell budding, cytoskeleton organization, cytokinesis, protein complex biogenesis, regulation of cell cycle kinase activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 13431 ORF|Verified 15 - C3 4 E 7 YLR200W YKE2 PFD6|GIM1 Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin protein folding, protein complex biogenesis cytoskeletal protein binding cytoplasm 14149 ORF|Verified 16 - C4 4 E 8 YCL025C AGP1 YCC5 Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) transmembrane transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 13432 ORF|Verified 15 - C4 4 E 9 YLR204W QRI5 COX24 Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA mRNA processing molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 14153 ORF|Verified 16 - C5 4 E 10 YCL026C-A FRM2 YCLX08C Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis; expression induced in cells treated with the mycotoxin patulin; has similarity to bacterial nitroreductases lipid metabolic process oxidoreductase activity nucleus, cytoplasm 13433 ORF|Verified 44 - A5 4 E 11 YLR205C HMX1 - ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants cofactor metabolic process, response to oxidative stress, response to chemical stimulus, cellular ion homeostasis oxidoreductase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 14154 ORF|Verified 16 - C6 4 E 12 YCL027W FUS1 - Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate cell morphogenesis, conjugation, response to chemical stimulus molecular_function membrane, site of polarized growth, plasma membrane 13434 ORF|Verified 15 - C6 4 E 13 YLR206W ENT2 - Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus cytoskeleton organization, membrane invagination, endocytosis other cell cortex, cytoskeleton, site of polarized growth, cytoplasm 14155 ORF|Verified 16 - C7 4 E 14 YCL028W RNQ1 [PIN(+)] [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate biological_process molecular_function cytoplasm 13435 ORF|Verified 15 - C7 4 E 15 YLR207W HRD3 - Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events proteolysis involved in cellular protein catabolic process ligase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 14156 ORF|Verified 16 - C8 4 E 16 YCL029C BIK1 PAC14|ARM5 Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 mitotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, conjugation, regulation of organelle organization, protein complex biogenesis, nucleus organization cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, chromosome, microtubule organizing center, site of polarized growth, cytoplasm 13436 ORF|Verified 15 - C8 4 E 17 YLR209C PNP1 - Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, hydrolase activity, acting on glycosyl bonds other 14158 ORF|Verified 16 - C9 4 E 18 YCL033C MXR2 MSRB Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm 13440 ORF|Uncharacterized 15 - C9 4 E 19 YLR210W CLB4 - B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation mitotic cell cycle, protein phosphorylation, cytoskeleton organization, regulation of organelle organization, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cytoskeleton, microtubule organizing center, cytoplasm 14159 ORF|Verified 16 - C10 4 E 20 YCL034W LSB5 - Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat membrane invagination, cytoskeleton organization, endocytosis molecular_function cell cortex, cytoskeleton, cytoplasm 13441 ORF|Verified 15 - C10 4 E 21 YLR211C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron biological_process molecular_function cytoplasm 14160 ORF|Uncharacterized 16 - C11 4 E 22 YCL036W GFD2 YCD6 Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation biological_process molecular_function cellular_component 13443 ORF|Verified 15 - C11 4 E 23 YLR213C CRR1 - Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation sporulation, cell wall organization or biogenesis hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cell wall 14162 ORF|Verified 16 - C12 4 E 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 F 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 F 2 YGR154C GTO1 - Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization cellular amino acid metabolic process transferase activity, transferring alkyl or aryl (other than methyl) groups peroxisome, cytoplasm 14784 ORF|Verified 13 - C1 4 F 3 YHR060W VMA22 VPH6|CEV1 Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) cellular ion homeostasis, protein complex biogenesis unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 11888 ORF|Verified 14 - C2 4 F 4 YGR157W CHO2 PEM1 Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis lipid metabolic process methyltransferase activity membrane, membrane fraction 14787 ORF|Verified 13 - C2 4 F 5 YHR061C GIC1 - Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain mitotic cell cycle, cell budding, cytokinesis, signaling, regulation of cell cycle enzyme regulator activity, enzyme binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 11889 ORF|Verified 14 - C3 4 F 6 YGR159C NSR1 SHE5 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, DNA binding nucleus, mitochondrion, nucleolus, cytoplasm 14789 ORF|Verified 13 - C3 4 F 7 YHR066W SSF1 - Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family ribosome assembly, conjugation, organelle assembly, ribosomal large subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus 11894 ORF|Verified 14 - C4 4 F 8 YGR160W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14790 - 13 - C4 4 F 9 YHR073W OSH3 - Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability invasive growth in response to glucose limitation, organelle fusion, lipid transport, membrane invagination, conjugation, exocytosis, pseudohyphal growth, nucleus organization, endocytosis lipid binding cell cortex, endoplasmic reticulum, cytoplasm 11901 ORF|Verified 14 - C5 4 F 10 YGR161C RTS3 - Putative component of the protein phosphatase type 2A complex biological_process molecular_function nucleus, cytoplasm 14791 ORF|Uncharacterized 13 - C5 4 F 11 YHR075C PPE1 YmS2|MRPS2 Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 11903 ORF|Verified 14 - C6 4 F 12 YGR163W GTR2 - Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD membrane invagination, transcription from RNA polymerase II promoter other nucleus, chromosome, vacuole, membrane, cytoplasm 14793 ORF|Verified 13 - C6 4 F 13 YHR076W PTC7 - Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope biological_process phosphatase activity nucleus, endomembrane system, mitochondrion, cytoplasm 11904 ORF|Verified 14 - C7 4 F 14 YGR164W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14794 - 13 - C7 4 F 15 YHR077C NMD2 SUP111|UPF2|SUA1|IFS1 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance RNA catabolic process molecular_function cytoplasm 11905 ORF|Verified 14 - C8 4 F 16 YGR165W MRPS35 - Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 14795 ORF|Verified 13 - C8 4 F 17 YHR078W - - High osmolarity-regulated gene of unknown function biological_process molecular_function membrane 11906 ORF|Uncharacterized 14 - C9 4 F 18 YGR166W TRS65 KRE11 Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Golgi vesicle transport, protein complex biogenesis enzyme regulator activity cytoplasm, Golgi apparatus 14796 ORF|Verified 13 - C9 4 F 19 YHR079C IRE1 ERN1 Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress carbohydrate metabolic process, protein phosphorylation, response to chemical stimulus, cell wall organization or biogenesis, signaling kinase activity, unfolded protein binding, nuclease activity nucleus 11907 ORF|Verified 14 - C10 4 F 20 YGR168C - - Putative protein of unknown function; YGR168C is not an essential gene biological_process molecular_function membrane 14798 ORF|Uncharacterized 13 - C10 4 F 21 YHR080C - - Protein of unknown function that may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ribosome, mitochondrion, cytoplasm 11908 ORF|Verified 14 - C11 4 F 22 YGR169C PUS6 - tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability tRNA processing, RNA modification isomerase activity mitochondrion, cytoplasm 14799 ORF|Verified 13 - C11 4 F 23 YHR081W LRP1 RRP47|YC1D Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination RNA catabolic process, snoRNA processing, rRNA processing RNA binding, DNA binding nucleus 11909 ORF|Verified 14 - C12 4 F 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 G 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 G 2 YCL039W GID7 MOH2 Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm 13446 ORF|Verified 15 - D1 4 G 3 YLR216C CPR6 CYP40 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity protein folding unfolded protein binding, isomerase activity cytoplasm 14165 ORF|Verified 16 - D2 4 G 4 YCL040W GLK1 HOR3 Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources carbohydrate metabolic process, carbohydrate transport, generation of precursor metabolites and energy kinase activity membrane fraction, cytoplasm 13447 ORF|Verified 15 - D2 4 G 5 YLR217W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 biological_process molecular_function cellular_component 14166 - 16 - D3 4 G 6 YCL042W - - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm 13449 ORF|Uncharacterized 15 - D3 4 G 7 YLR218C COA4 CMC3 Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors mitochondrion organization, protein complex biogenesis molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 14167 ORF|Uncharacterized 16 - D4 4 G 8 YCL044C MGR1 - Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm 13451 ORF|Verified 15 - D4 4 G 9 YLR219W MSC3 - Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate meiotic cell cycle, DNA recombination molecular_function cellular_component 14168 ORF|Verified 16 - D5 4 G 10 YCL045C EMC1 - Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 13452 ORF|Uncharacterized 15 - D5 4 G 11 YLR220W CCC1 - Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation cellular ion homeostasis, ion transport transmembrane transporter activity membrane, membrane fraction, vacuole, cytoplasm, Golgi apparatus 14169 ORF|Verified 16 - D6 4 G 12 YCL046W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C biological_process molecular_function cellular_component 13453 - 15 - D6 4 G 13 YLR221C RSA3 - Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 14170 ORF|Verified 16 - D7 4 G 14 YCL047C POF1 - Protein involved in the filamentation pathway; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion invasive growth in response to glucose limitation, pseudohyphal growth molecular_function cellular_component 13454 ORF|Uncharacterized 15 - D7 4 G 15 YLR224W - - F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene proteolysis involved in cellular protein catabolic process, response to chemical stimulus protein binding, bridging, ligase activity other 14173 ORF|Uncharacterized 16 - D8 4 G 16 YCL048W SPS22 - Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane 13455 ORF|Verified 15 - D8 4 G 17 YLR225C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene biological_process molecular_function cytoplasm 14174 ORF|Uncharacterized 16 - D9 4 G 18 YCL049C - - Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane fraction 13456 ORF|Uncharacterized 15 - D9 4 G 19 YLR226W BUR2 CST4 Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II mitotic cell cycle, protein phosphorylation, protein alkylation, organelle fission, chromatin organization, regulation of organelle organization, histone modification, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter enzyme regulator activity nucleus 14175 ORF|Verified 16 - D10 4 G 20 YCL050C APA1 DTP1 Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p nucleobase-containing small molecule metabolic process other nucleus, cytoplasm 13457 ORF|Verified 15 - D10 4 G 21 YLR227C ADY4 - Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane sporulation structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm 14176 ORF|Verified 16 - D11 4 G 22 YCL051W LRE1 - Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway cell wall organization or biogenesis molecular_function cellular_component 13458 ORF|Verified 15 - D11 4 G 23 YKL001C MET14 - Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism cellular amino acid metabolic process kinase activity cytoplasm 14849 ORF|Verified 16 - D12 4 G 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 H 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 H 2 YGR173W RBG2 GIR1 Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein cytoplasmic translation other cytoplasm 14803 ORF|Uncharacterized 13 - D1 4 H 3 YHR086W NAM8 MUD15|MRE2 RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 11914 ORF|Verified 14 - D2 4 H 4 YGR176W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14806 - 13 - D2 4 H 5 YHR087W RTC3 - Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity other molecular_function nucleus, cytoplasm 11915 ORF|Verified 14 - D3 4 H 6 YGR177C ATF2 - Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking lipid metabolic process, response to chemical stimulus transferase activity, transferring acyl groups membrane, endomembrane system, endoplasmic reticulum, cytoplasm 14807 ORF|Verified 13 - D3 4 H 7 YHR092C HXT4 RAG1|LGT1 High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 11920 ORF|Verified 14 - D4 4 H 8 YGR178C PBP1 MRS16 Component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation regulation of translation, mRNA processing molecular_function nucleus, mitochondrion, cytoplasm 14808 ORF|Verified 13 - D4 4 H 9 YHR093W AHT1 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region biological_process molecular_function cellular_component 11921 - 14 - D5 4 H 10 YGR181W TIM13 - Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein targeting, mitochondrion organization protein transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 14811 ORF|Verified 13 - D5 4 H 11 YHR094C HXT1 HOR4 Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 11922 ORF|Verified 14 - D6 4 H 12 YGR182C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W biological_process molecular_function cellular_component 14812 - 13 - D6 4 H 13 YHR095W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11923 - 14 - D7 4 H 14 YGR183C QCR9 UCR9 Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 14813 ORF|Verified 13 - D7 4 H 15 YHR096C HXT5 - Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs carbohydrate transport transmembrane transporter activity membrane, membrane fraction, plasma membrane 11924 ORF|Verified 14 - D8 4 H 16 YGR184C UBR1 PTR1 E3 ubiquitin ligase (N-recognin), forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) protein modification by small protein conjugation or removal, regulation of transport, proteolysis involved in cellular protein catabolic process, response to chemical stimulus ligase activity cytoplasm 14814 ORF|Verified 13 - D8 4 H 17 YHR097C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 11925 ORF|Uncharacterized 14 - D9 4 H 18 YGR187C HGH1 - Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16) biological_process molecular_function cytoplasm 14817 ORF|Verified 13 - D9 4 H 19 YHR100C GEP4 - Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT carbohydrate metabolic process, lipid metabolic process phosphatase activity mitochondrion, cytoplasm 11928 ORF|Uncharacterized 14 - D10 4 H 20 YGR189C CRH1 - Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups cell wall 14819 ORF|Verified 13 - D10 4 H 21 YHR103W SBE22 - Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth cell wall organization or biogenesis molecular_function cytoplasm 11931 ORF|Verified 14 - D11 4 H 22 YGR192C TDH3 GAPDH|GPD|SSS2|HSP36|HSP35|GLD1 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall carbohydrate metabolic process, generation of precursor metabolites and energy oxidoreductase activity cell wall, membrane fraction, mitochondrion, cytoplasm 14822 ORF|Verified 13 - D11 4 H 23 YHR104W GRE3 - Aldose reductase involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway carbohydrate metabolic process, response to oxidative stress, response to chemical stimulus RNA binding, oxidoreductase activity, mRNA binding nucleus, cytoplasm 11932 ORF|Verified 14 - D12 4 H 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 I 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 I 2 YCL056C PEX34 - Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation peroxisome organization molecular_function peroxisome, membrane, cytoplasm 13463 ORF|Uncharacterized 15 - E1 4 I 3 YKL007W CAP1 - Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches cytoskeleton organization, regulation of organelle organization, protein complex biogenesis cytoskeletal protein binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm 14856 ORF|Verified 16 - E2 4 I 4 YCL057W PRD1 - Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins other peptidase activity mitochondrial envelope, mitochondrion, cytoplasm 13464 ORF|Verified 15 - E2 4 I 5 YKL008C LAC1 DGT1 Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p lipid metabolic process transferase activity, transferring acyl groups membrane, endoplasmic reticulum, cytoplasm 14857 ORF|Verified 16 - E3 4 I 6 YCL061C MRC1 YCL060C S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres mitotic cell cycle, DNA repair, telomere organization, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 13467 ORF|Verified 15 - E4 4 I 7 YKL009W MRT4 - Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus rRNA processing, organelle assembly, ribosomal large subunit biogenesis, RNA catabolic process, ribosome assembly RNA binding, rRNA binding nucleus, nucleolus 14858 ORF|Verified 16 - E4 4 I 8 YCL061C MRC1 YCL060C S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres mitotic cell cycle, DNA repair, telomere organization, organelle fission, DNA replication, chromosome segregation, response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 13468 ORF|Verified 15 - E4 4 I 9 YKL010C UFD4 - Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins protein modification by small protein conjugation or removal ligase activity mitochondrion, cytoplasm 14859 ORF|Verified 16 - E5 4 I 10 YCL062W - - - - - - 13469 - 4 I 11 YKL011C CCE1 MGT1 Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA mitochondrion organization nuclease activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 14860 ORF|Verified 16 - E6 4 I 12 YCL063W VAC17 YCL062W Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p vacuole organization, organelle inheritance other membrane, vacuole, cytoplasm 13470 ORF|Verified 15 - E6 4 I 13 YKL015W PUT3 - Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitogen sources; has a Zn(2)-Cys(6) binuclear cluster domain transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 14864 ORF|Verified 16 - E7 4 I 14 YCL064C CHA1 - Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 13471 ORF|Verified 15 - E7 4 I 15 YKL017C HCS1 DIP1 Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities DNA replication helicase activity, ATPase activity nucleus, chromosome 14866 ORF|Verified 16 - E8 4 I 16 YCL069W VBA3 - Permease of basic amino acids in the vacuolar membrane transmembrane transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm 13476 ORF|Verified 15 - E8 4 I 17 YKL020C SPT23 - ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting lipid metabolic process, transcription from RNA polymerase II promoter DNA binding nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 14869 ORF|Verified 16 - E9 4 I 18 YCR001W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene biological_process molecular_function cellular_component 13481 - 15 - E9 4 I 19 YKL023W - - Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 14872 ORF|Uncharacterized 16 - E10 4 I 20 YCR004C YCP4 - Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other membrane, membrane fraction, mitochondrion, cytoplasm 13484 ORF|Verified 15 - E10 4 I 21 YKL025C PAN3 ECM35 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes response to DNA damage stimulus, DNA repair, mRNA processing nuclease activity cytoplasm 14874 ORF|Verified 16 - E11 4 I 22 YCR005C CIT2 - Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors carbohydrate metabolic process, cellular amino acid metabolic process transferase activity, transferring acyl groups peroxisome, mitochondrion, cytoplasm 13485 ORF|Verified 15 - E11 4 I 23 YKL026C GPX1 - Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity cellular_component 14875 ORF|Verified 16 - E12 4 I 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 J 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 J 2 YGR194C XKS1 - Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains carbohydrate metabolic process kinase activity cytoplasm 14824 ORF|Verified 13 - E1 4 J 3 YHR106W TRR2 - Mitochondrial thioredoxin reductase involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD response to oxidative stress, response to chemical stimulus oxidoreductase activity mitochondrion, cytoplasm 11934 ORF|Verified 14 - E2 4 J 4 YGR196C FYV8 - Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cytoplasm 14826 ORF|Verified 13 - E2 4 J 5 YHR108W GGA2 - Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin protein targeting, proteolysis involved in cellular protein catabolic process, Golgi vesicle transport, endosome transport lipid binding, small conjugating protein binding Golgi apparatus, cytoplasm 11936 ORF|Verified 14 - E3 4 J 6 YGR197C SNG1 - Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance nucleobase-containing compound transport molecular_function membrane, plasma membrane 14827 ORF|Verified 13 - E3 4 J 7 YHR109W CTM1 - Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth peptidyl-amino acid modification, protein alkylation methyltransferase activity cytoplasm 11937 ORF|Verified 14 - E4 4 J 8 YGR199W PMT6 - Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups membrane, endoplasmic reticulum, cytoplasm 14829 ORF|Verified 13 - E4 4 J 9 YHR110W ERP5 - Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport other molecular_function membrane 11938 ORF|Verified 14 - E5 4 J 10 YGR200C ELP2 KTI3|TOT2 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin tRNA processing, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, RNA modification molecular_function nucleus, cytoplasm 14830 ORF|Verified 13 - E5 4 J 11 YHR111W UBA4 YHR1 Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p invasive growth in response to glucose limitation, cell budding, response to oxidative stress, response to chemical stimulus, tRNA processing, protein modification by small protein conjugation or removal, pseudohyphal growth, RNA modification nucleotidyltransferase activity cytoplasm 11939 ORF|Verified 14 - E6 4 J 12 YGR202C PCT1 CCT1|BSR2 Cholinephosphate cytidylyltransferase, also known as CTP:phosphocholine cytidylyltransferase, rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding lipid metabolic process nucleotidyltransferase activity nucleus, endomembrane system, cytoplasm, Golgi apparatus 14832 ORF|Verified 13 - E6 4 J 13 YHR112C - - Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway other lyase activity nucleus, cytoplasm 11940 ORF|Uncharacterized 14 - E7 4 J 14 YGR203W YCH1 - Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus other phosphatase activity nucleus, cytoplasm 14833 ORF|Uncharacterized 13 - E7 4 J 15 YHR113W APE4 - Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family other peptidase activity vacuole, ribosome, cytoplasm 11941 ORF|Verified 14 - E8 4 J 16 YGR205W TDA10 - ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele biological_process other nucleus, cytoplasm 14835 ORF|Verified 13 - E8 4 J 17 YHR114W BZZ1 LSB7 SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins cytoskeleton organization, membrane invagination, response to osmotic stress, endocytosis molecular_function cell cortex, cytoskeleton, site of polarized growth, cytoplasm 11942 ORF|Verified 14 - E9 4 J 18 YGR206W MVB12 - ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin protein targeting, endosome transport, protein complex biogenesis small conjugating protein binding membrane, cytoplasm 14836 ORF|Verified 13 - E9 4 J 19 YHR115C DMA1 CHF1 Protein involved in ubiquitin ligation; plays a role in regulating spindle position and orientation; functionally redundant with Dma2p; orthologous to human RNF8 protein, also has sequence similarity to human Chfr. mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis, protein modification by small protein conjugation or removal, regulation of cell cycle ligase activity cytoplasm 11943 ORF|Verified 14 - E10 4 J 20 YGR207C CIR1 - Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response biological_process molecular_function mitochondrion, cytoplasm 14837 ORF|Verified 13 - E10 4 J 21 YHR116W COX23 - Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm 11944 ORF|Verified 14 - E11 4 J 22 YGR208W SER2 - Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source cellular amino acid metabolic process phosphatase activity nucleus, cytoplasm 14838 ORF|Verified 13 - E11 4 J 23 YHR117W TOM71 TOM72 Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins biological_process protein transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 11945 ORF|Verified 14 - E12 4 J 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 K 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 K 2 YCR007C - - Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene biological_process molecular_function cellular_component 13487 ORF|Uncharacterized 15 - F1 4 K 3 YKL029C MAE1 - Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 14878 ORF|Verified 16 - F2 4 K 4 YCR008W SAT4 HAL4 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p mitotic cell cycle, cellular ion homeostasis, protein dephosphorylation kinase activity cellular_component 13488 ORF|Verified 15 - F2 4 K 5 YKL031W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species biological_process molecular_function cellular_component 14880 - 16 - F3 4 K 6 YCR009C RVS161 SPE161|FUS7|END6 Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress cytoskeleton organization, membrane invagination, conjugation, response to starvation, response to osmotic stress, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, membrane, site of polarized growth, cytoplasm 13489 ORF|Verified 15 - F3 4 K 7 YKL032C IXR1 ORD1 Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b response to DNA damage stimulus, DNA repair, transcription from RNA polymerase II promoter, response to chemical stimulus DNA binding nucleus, chromosome 14881 ORF|Verified 16 - F4 4 K 8 YCR010C ADY2 ATO1 Acetate transporter required for normal sporulation; phosphorylated in mitochondria transmembrane transport, ion transport transmembrane transporter activity membrane fraction, mitochondrion, membrane, cytoplasm, plasma membrane 13490 ORF|Verified 15 - F4 4 K 9 YKL034W TUL1 - Golgi-localized RING-finger ubiquitin ligase (E3), involved in ubiquitinating and sorting membrane proteins that contain polar transmembrane domains to multivesicular bodies for delivery to the vacuole for quality control purposes protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process ligase activity membrane, cytoplasm, Golgi apparatus 14883 ORF|Verified 16 - F5 4 K 10 YCR011C ADP1 - Putative ATP-dependent permease of the ABC transporter family of proteins transmembrane transport ATPase activity, transmembrane transporter activity membrane, endoplasmic reticulum, cytoplasm 13491 ORF|Verified 15 - F5 4 K 11 YKL037W AIM26 - Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT other molecular_function membrane, mitochondrion, cytoplasm 14886 ORF|Uncharacterized 16 - F6 4 K 12 YCR014C POL4 POLX DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta response to DNA damage stimulus, DNA repair nucleotidyltransferase activity nucleus 13494 ORF|Verified 15 - F6 4 K 13 YKL038W RGT1 - Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor carbohydrate metabolic process, carbohydrate transport, regulation of transport DNA binding nucleus 14887 ORF|Verified 16 - F7 4 K 14 YCR015C - - Putative protein of unknown function; YCR015C is not an essential gene biological_process molecular_function cellular_component 13495 ORF|Uncharacterized 15 - F7 4 K 15 YKL039W PTM1 - Protein of unknown function, copurifies with late Golgi vesicles containing the v-SNARE Tlg2p biological_process molecular_function membrane 14888 ORF|Verified 16 - F8 4 K 16 YCR016W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus 13496 ORF|Uncharacterized 15 - F8 4 K 17 YKL040C NFU1 NUB1 Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria cofactor metabolic process, cellular ion homeostasis molecular_function mitochondrion, cytoplasm 14889 ORF|Verified 16 - F9 4 K 18 YCR017C CWH43 - Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion lipid metabolic process, cell wall organization or biogenesis, protein lipidation molecular_function cellular bud, membrane, site of polarized growth, plasma membrane 13497 ORF|Verified 15 - F9 4 K 19 YKL041W VPS24 VPL26|DID3 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway endosome transport, vesicle organization, proteolysis involved in cellular protein catabolic process molecular_function membrane, cytoplasm 14890 ORF|Verified 16 - F10 4 K 20 YCR019W MAK32 - Protein necessary for structural stability of L-A double-stranded RNA-containing particles other molecular_function cellular_component 13499 ORF|Verified 15 - F10 4 K 21 YKL043W PHD1 - Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate pseudohyphal growth, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 14892 ORF|Verified 16 - F11 4 K 22 YCR020C PET18 HIT2 Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin vitamin metabolic process molecular_function cytoplasm 13500 ORF|Verified 15 - F11 4 K 23 YKL044W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 14893 - 16 - F12 4 K 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 L 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 L 2 YGR212W SLI1 - N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin response to chemical stimulus transferase activity, transferring acyl groups nucleus, membrane, endomembrane system, plasma membrane 14842 ORF|Verified 13 - F1 4 L 3 YHR123W EPT1 - sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability lipid metabolic process other membrane, cytoplasm, Golgi apparatus 11951 ORF|Verified 14 - F2 4 L 4 YGR213C RTA1 - Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein biological_process molecular_function membrane, plasma membrane 14843 ORF|Verified 13 - F2 4 L 5 YHR124W NDT80 - Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) meiotic cell cycle nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 11952 ORF|Verified 14 - F3 4 L 6 YGR214W RPS0A S0A|YST1|NAB1A|NAB1 Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal cytoplasmic translation, rRNA processing, organelle assembly, nucleobase-containing compound transport, ribosomal small subunit biogenesis, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 14844 ORF|Verified 13 - F3 4 L 7 YHR125W - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11953 - 14 - F4 4 L 8 YGR217W CCH1 - Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together ion transport transmembrane transporter activity membrane, plasma membrane 14847 ORF|Verified 13 - F4 4 L 9 YHR126C ANS1 - Putative protein of unknown function; transcription dependent upon Azf1p biological_process molecular_function cellular_component 11954 ORF|Uncharacterized 14 - F5 4 L 10 YHL047C ARN2 TAF1 Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C cofactor metabolic process, cellular ion homeostasis, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, plasma membrane, cytoplasm 10916 ORF|Verified 13 - F5 4 L 11 YHR129C ARP1 ACT5 Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin mitotic cell cycle, cytoskeleton organization structural molecule activity cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 11957 ORF|Verified 14 - F6 4 L 12 YHL046C PAU13 - Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock biological_process molecular_function cellular_component 10917 ORF|Uncharacterized 13 - F6 4 L 13 YHR130C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11958 - 14 - F7 4 L 14 YHL045W - - Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component 10918 - 13 - F7 4 L 15 YHR132C ECM14 - Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly cell wall organization or biogenesis peptidase activity vacuole, cytoplasm 11960 ORF|Verified 14 - F8 4 L 16 YHL044W - - Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern biological_process molecular_function membrane, plasma membrane 10919 ORF|Uncharacterized 13 - F8 4 L 17 YHR133C NSG1 - Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins lipid metabolic process unfolded protein binding membrane, endoplasmic reticulum, cytoplasm 11961 ORF|Verified 14 - F9 4 L 18 YHL043W ECM34 - Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins cell wall organization or biogenesis molecular_function cellular_component 10920 ORF|Verified 13 - F9 4 L 19 YHR134W WSS1 - Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response response to DNA damage stimulus, protein modification by small protein conjugation or removal molecular_function nucleus, endomembrane system 11962 ORF|Verified 14 - F10 4 L 20 YHL040C ARN1 - Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores cellular ion homeostasis, ion transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, plasma membrane, cytoplasm 10923 ORF|Verified 13 - F10 4 L 21 YHR135C YCK1 CKI2 Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p protein phosphorylation, cell morphogenesis, cytokinesis, response to chemical stimulus, membrane invagination, endocytosis kinase activity membrane fraction, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 11963 ORF|Verified 14 - F11 4 L 22 YHL037C - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 10926 - 13 - F11 4 L 23 YHR136C SPL2 - Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm other enzyme regulator activity cytoplasm 11964 ORF|Verified 14 - F12 4 L 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 M 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 M 2 YCR021C HSP30 YRO1 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase nucleobase-containing small molecule metabolic process molecular_function membrane, membrane fraction, plasma membrane 13502 ORF|Verified 15 - G1 4 M 3 YKL047W - - Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 14896 ORF|Uncharacterized 16 - G2 4 M 4 YCR022C - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene biological_process molecular_function cellular_component 13503 - 15 - G2 4 M 5 YKL048C ELM1 LDB9 Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases; forms part of the bud neck ring response to chemical stimulus, response to osmotic stress, carbohydrate metabolic process, cell budding, cell morphogenesis, protein phosphorylation, cytokinesis, regulation of protein modification process, pseudohyphal growth, regulation of cell cycle kinase activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 14897 ORF|Verified 16 - G3 4 M 6 YCR023C - - Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 13504 ORF|Uncharacterized 15 - G3 4 M 7 YKL050C - - Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p biological_process molecular_function cellular_component 14899 ORF|Verified 16 - G4 4 M 8 YLR420W URA4 - Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate nucleobase-containing small molecule metabolic process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds nucleus, cytoplasm 14081 ORF|Verified 15 - G4 4 M 9 YKL051W SFK1 - Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane vacuole organization, cytoskeleton organization, signaling molecular_function membrane, plasma membrane 14900 ORF|Verified 16 - G5 4 M 10 YLR451W LEU3 - Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 14082 ORF|Verified 15 - G5 4 M 11 YKL053W - - Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 biological_process molecular_function membrane 14902 - 16 - G6 4 M 12 YLR126C - - Putative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein cellular ion homeostasis molecular_function cytoplasm 14083 ORF|Uncharacterized 15 - G6 4 M 13 YKL054C DEF1 VID31 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis response to DNA damage stimulus, DNA repair, telomere organization, proteolysis involved in cellular protein catabolic process molecular_function nucleus 14903 ORF|Verified 16 - G7 4 M 14 YLR128W DCN1 - Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation regulation of protein modification process, protein modification by small protein conjugation or removal small conjugating protein binding, ligase activity cellular_component 14085 ORF|Verified 15 - G7 4 M 15 YKL055C OAR1 - Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p lipid metabolic process, generation of precursor metabolites and energy, cellular respiration transferase activity, transferring acyl groups, oxidoreductase activity mitochondrion, cytoplasm 14904 ORF|Verified 16 - G8 4 M 16 YLR130C ZRT2 - Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane 14087 ORF|Verified 15 - G8 4 M 17 YKL056C TMA19 MMI1|RBF18 Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress cytoplasmic translation, response to oxidative stress, response to chemical stimulus molecular_function ribosome, mitochondrion, cytoplasm 14905 ORF|Verified 16 - G9 4 M 18 YLR131C ACE2 - Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit of Ace2p during the M-to-G1 transition, causing its specific localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of the RAM network that regulates cellular polarity and morphogenesis mitotic cell cycle, cytokinesis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 14088 ORF|Verified 15 - G9 4 M 19 YKL057C NUP120 RAT2 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; the Nup84 subcomplex has a role in transcription elongation response to heat, protein targeting, ribosomal subunit export from nucleus, nucleobase-containing compound transport, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane 14906 ORF|Verified 16 - G10 4 M 20 YLR133W CKI1 - Choline kinase, catalyzing the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway lipid metabolic process kinase activity cytoplasm 14090 ORF|Verified 15 - G10 4 M 21 YKL061W BLI1 - Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome biological_process molecular_function cytoplasm 14910 ORF|Uncharacterized 16 - G11 4 M 22 YLR134W PDC5 - Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism carbohydrate metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process lyase activity nucleus, cytoplasm 14091 ORF|Verified 15 - G11 4 M 23 YKL062W MSN4 - Transcriptional activator related to Msn2p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression response to heat, chromatin organization, response to oxidative stress, response to chemical stimulus, response to starvation, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 14911 ORF|Verified 16 - G12 4 M 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 N 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 N 2 YHL035C VMR1 - Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions response to chemical stimulus transmembrane transporter activity, ATPase activity vacuole, ribosome, mitochondrion, membrane, cytoplasm 10928 ORF|Verified 13 - G1 4 N 3 YHR138C - - Putative protein of unknown function; has similarity to Pbi2p; double null mutant lacking Pbi2p and Yhr138p exhibits highly fragmented vacuoles vacuole organization, membrane fusion enzyme regulator activity cellular_component 11966 ORF|Uncharacterized 14 - G2 4 N 4 YHL034C SBP1 SSB1|SSBR1 Putative RNA binding protein; involved in translational repression and found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 regulation of translation RNA binding nucleus, nucleolus, cytoplasm 10929 ORF|Verified 13 - G2 4 N 5 YHR139C SPS100 - Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin sporulation, cell wall organization or biogenesis molecular_function cell wall 11967 ORF|Verified 14 - G3 4 N 6 YHL033C RPL8A rp6|YL5|L8A|L4A|MAK7 Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 10930 ORF|Verified 13 - G3 4 N 7 YHR139C-A - - Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 11968 - 14 - G4 4 N 8 YHL032C GUT1 - Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p carbohydrate metabolic process kinase activity mitochondrion, cytoplasm 10931 ORF|Verified 13 - G4 4 N 9 YHR142W CHS7 - Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER carbohydrate metabolic process, Golgi vesicle transport, protein folding, cell wall organization or biogenesis unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 12835 ORF|Verified 14 - G5 4 N 10 YHL031C GOS1 - v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 membrane fusion, Golgi vesicle transport, organelle fusion, vesicle organization other membrane, cytoplasm, Golgi apparatus 10932 ORF|Verified 13 - G5 4 N 11 YHR143W DSE2 - Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP pseudohyphal growth, cytokinesis hydrolase activity, acting on glycosyl bonds cell wall, extracellular region 12836 ORF|Verified 14 - G6 4 N 12 YHL028W WSC4 YFW1|YHC8 ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 response to heat, protein targeting, transmembrane transport signal transducer activity membrane fraction, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10935 ORF|Verified 13 - G6 4 N 13 YHR151C MTC6 - Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component 12845 ORF|Uncharacterized 14 - G7 4 N 14 YHL027W RIM101 RIM1 Transcriptional repressor involved in response to pH and in cell wall construction; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by proteolytic processing; similar to A. nidulans PacC meiotic cell cycle, sporulation, cytokinesis, cell wall organization or biogenesis, transcription from RNA polymerase II promoter, response to chemical stimulus DNA binding nucleus 10936 ORF|Verified 13 - G7 4 N 15 YHR152W SPO12 - Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis mitotic cell cycle, meiotic cell cycle, regulation of cell cycle molecular_function nucleus, nucleolus 12846 ORF|Verified 14 - G8 4 N 16 YHL026C - - Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) biological_process molecular_function cellular_component 10937 ORF|Uncharacterized 13 - G8 4 N 17 YHR153C SPO16 - Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation meiotic cell cycle, sporulation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 12847 ORF|Verified 14 - G9 4 N 18 YHL023C NPR3 RMD11 Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair meiotic cell cycle, response to starvation, signaling molecular_function membrane, vacuole, cytoplasm 10940 ORF|Verified 13 - G9 4 N 19 YHR154W RTT107 ESC4 Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition response to DNA damage stimulus, DNA repair, transposition molecular_function nucleus 12848 ORF|Verified 14 - G10 4 N 20 YHL022C SPO11 - Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation meiotic cell cycle nuclease activity nucleus, chromosome 10941 ORF|Verified 13 - G10 4 N 21 YHR155W YSP1 - Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone biological_process molecular_function mitochondrion, cytoplasm 12849 ORF|Verified 14 - G11 4 N 22 YHL020C OPI1 - Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance response to chemical stimulus, lipid metabolic process, transcription from RNA polymerase II promoter, signaling protein binding transcription factor activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 10943 ORF|Verified 13 - G11 4 N 23 YHR156C LIN1 SNU40 Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication biological_process other nucleus, chromosome 12850 ORF|Verified 14 - G12 4 N 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 O 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 O 2 YLR136C TIS11 CTH2 mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis RNA catabolic process, cellular ion homeostasis RNA binding, mRNA binding nucleus, cytoplasm 14093 ORF|Verified 15 - H1 4 O 3 YKL064W MNR2 - Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 14913 ORF|Verified 16 - H2 4 O 4 YLR137W RKM5 - Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species protein alkylation methyltransferase activity cellular_component 14094 ORF|Uncharacterized 15 - H2 4 O 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 O 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 O 7 YKL065C YET1 - Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function membrane, endomembrane system, ribosome, endoplasmic reticulum, cytoplasm 14914 ORF|Verified 16 - H4 4 O 8 YLR138W NHA1 - Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH cellular ion homeostasis, response to osmotic stress transmembrane transporter activity membrane, plasma membrane 14095 ORF|Verified 15 - H4 4 O 9 YKL066W - - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 biological_process molecular_function cellular_component 14915 - 16 - H5 4 O 10 YLR142W PUT1 - Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 14099 ORF|Verified 15 - H5 4 O 11 YKL067W YNK1 NDK1 Nucleoside diphosphate kinase, catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate response to DNA damage stimulus, nucleobase-containing small molecule metabolic process kinase activity mitochondrial envelope, mitochondrion, cytoplasm 14916 ORF|Verified 16 - H6 4 O 12 YLR143W - - Putative protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; YLR143W is not an essential gene biological_process molecular_function cytoplasm 14100 ORF|Uncharacterized 15 - H6 4 O 13 YKL069W - fRMsr|YKG9 Methionine-R-sulfoxide reductase, reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress response to oxidative stress, response to chemical stimulus oxidoreductase activity nucleus, cytoplasm 14918 ORF|Uncharacterized 16 - H7 4 O 14 YLR149C - - Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene biological_process molecular_function cellular_component 14106 ORF|Uncharacterized 15 - H7 4 O 15 YKL070W - - Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 14919 ORF|Uncharacterized 16 - H8 4 O 16 YLR150W STM1 MPT4 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure translational initiation, telomere organization, regulation of translation, translational elongation, signaling DNA binding ribosome, cytoplasm 14107 ORF|Verified 15 - H8 4 O 17 YKL071W - - Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function membrane, cytoplasm 14920 ORF|Uncharacterized 16 - H9 4 O 18 YLR151C PCD1 - Peroxisomal nudix pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids biological_process other peroxisome, cytoplasm 14108 ORF|Verified 15 - H9 4 O 19 YKL072W STB6 - Protein that binds Sin3p in a two-hybrid assay biological_process molecular_function cellular_component 14921 ORF|Verified 16 - H10 4 O 20 YLR152C - - Putative protein of unknown function; YLR152C is not an essential gene biological_process molecular_function membrane 14109 ORF|Uncharacterized 15 - H10 4 O 21 YKL073W LHS1 SSI1|CER1 Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway protein targeting, transmembrane transport, response to chemical stimulus unfolded protein binding, enzyme regulator activity endoplasmic reticulum, cytoplasm 14922 ORF|Verified 16 - H11 4 O 22 YLR154C RNH203 Rnh2C Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome RNA catabolic process, DNA replication nuclease activity nucleus, cytoplasm 14111 ORF|Verified 15 - H11 4 O 23 YKL074C MUD2 - Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 RNA splicing, mRNA processing RNA binding nucleus 14923 ORF|Verified 16 - H12 4 O 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 4 YOR128C ADE2 - Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine nucleobase-containing small molecule metabolic process lyase activity cytoplasm 12384 ORF|Verified 42 - E4 4 P 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 4 P 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 2 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 3 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 4 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 5 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 6 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 7 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 8 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 9 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 10 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 11 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 12 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 13 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 14 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 15 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 16 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 17 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 18 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 19 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 20 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 21 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 22 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 23 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 A 24 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 B 1 YOR202W HIS3-BLANK HIS10 "Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p" 12458 ORF|Verified 19 - B6 5 B 2 YKL075C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin response to chemical stimulus molecular_function cytoplasm 14924 ORF|Uncharacterized 17 - A1 5 B 3 YGR055W MUP1 - High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake amino acid transport transmembrane transporter activity membrane, plasma membrane 14685 ORF|Verified 18 - A2 5 B 4 YKL076C PSY1 - Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C biological_process molecular_function cellular_component 14925 - 17 - A2 5 B 5 YGR056W RSC1 - Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook response to DNA damage stimulus, DNA repair, chromatin organization, sporulation, transcription elongation, DNA-dependent, transcription from RNA polymerase II promoter ATPase activity nucleus 14686 ORF|Verified 18 - A3 5 B 6 YKL077W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function vacuole, cytoplasm 14926 ORF|Uncharacterized 17 - A3 5 B 7 YGR057C LST7 - Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Golgi vesicle transport protein transporter activity membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm 14687 ORF|Verified 18 - A4 5 B 8 YKL079W SMY1 - Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins exocytosis other cellular bud, site of polarized growth 14928 ORF|Verified 17 - A4 5 B 9 YGR058W PEF1 - Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2 cell budding, cytokinesis ion binding cellular bud, nucleus, site of polarized growth, cytoplasm 14688 ORF|Verified 18 - A5 5 B 10 YKL080W VMA5 VAT3|CSL5 Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane cellular ion homeostasis ATPase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 14929 ORF|Verified 17 - A5 5 B 11 YGR059W SPR3 - Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI sporulation structural molecule activity cell wall, membrane, cell cortex, cytoskeleton, cytoplasm 14689 ORF|Verified 18 - A6 5 B 12 YKL081W TEF4 EFC1 Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex translational elongation RNA binding, translation factor activity, nucleic acid binding ribosome, mitochondrion, cytoplasm 14930 ORF|Verified 17 - A6 5 B 13 YGR061C ADE6 - Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process ligase activity cytoplasm 14691 ORF|Verified 18 - A7 5 B 14 YKL084W HOT13 - Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc protein targeting, mitochondrion organization, transmembrane transport ion binding mitochondrial envelope, mitochondrion, cytoplasm 14933 ORF|Verified 17 - A7 5 B 15 YGR0