LOCUS PWJ1077.GB 5784 BP DS-DNA CIRCULAR SYN 14-SEP-1995 DEFINITION Yeast integrative vector pWJ1077 with Kluveromyces lactis URA3 marker and Saccharomyces cerevisiae HIS3 marker, complete sequence. ACCESSION Not submitted as of 4/2002 KEYWORDS K. lactis URA3 adaptamer gene disruption SOURCE Cloning vector pRS303 ORGANISM Saccharomyces cerevisiae REFERENCE 1 AUTHORS Reid RJ, Sunjevaric I, Kedacche M, Rothstein R. TITLE Efficient PCR-based gene disruption in Saccharomyces strains using intergenic primers. JOURNAL Yeast 2002 Mar 15;19(4):319-28 FEATURES Location/Qualifiers CDS 4734..5594 /note="bla (amp-R)" CDS 503..1162 /note="HIS3" primer_bind complement(2894..2918) /note="Kli-3'" primer_bind 2471..2495 /note="Kli-5'" CDS <2366..>3168 /note="K.lactis URA3" primer_bind 1993..2016 /note="c-Kl" primer_bind complement(3522..3545) /note="d-Kl" primer_bind 2083..2102 /note="d2-Kl" primer_bind complement(3488..3509) /note="u2-Kl" frag 3361..3515 /note="Repeat" frag 2083..2225 /note="Repeat" BASE COUNT 1539 A 1281 C 1414 G 1550 T 0 OTHER ORIGIN - 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATAATTC CGTTTTAAGA GCTTGGTGAG CGCTAGGAGT CACTGCCAGG TATCGTTTGA 241 ACACGGCATT AGTCAGGGAA GTCATAACAC AGTCCTTTCC CGCAATTTTC TTTTTCTATT 301 ACTCTTGGCC TCCTCTAGTA CACTCTATAT TTTTTTATGC CTCGGTAATG ATTTTCATTT 361 TTTTTTTTCC ACCTAGCGGA TGACTCTTTT TTTTTCTTAG CGATTGGCAT TATCACATAA 421 TGAATTATAC ATTATATAAA GTAATGTGAT TTCTTCGAAG AATATACTAA AAAATGAGCA 481 GGCAAGATAA ACGAAGGCAA AGATGACAGA GCAGAAAGCC CTAGTAAAGC GTATTACAAA 541 TGAAACCAAG ATTCAGATTG CGATCTCTTT AAAGGGTGGT CCCCTAGCGA TAGAGCACTC 601 GATCTTCCCA GAAAAAGAGG CAGAAGCAGT AGCAGAACAG GCCACACAAT CGCAAGTGAT 661 TAACGTCCAC ACAGGTATAG GGTTTCTGGA CCATATGATA CATGCTCTGG CCAAGCATTC 721 CGGCTGGTCG CTAATCGTTG AGTGCATTGG TGACTTACAC ATAGACGACC ATCACACCAC 781 TGAAGACTGC GGGATTGCTC TCGGTCAAGC TTTTAAAGAG GCCCTACTGG CGCGTGGAGT 841 AAAAAGGTTT GGATCAGGAT TTGCGCCTTT GGATGAGGCA CTTTCCAGAG CGGTGGTAGA 901 TCTTTCGAAC AGGCCGTACG CAGTTGTCGA ACTTGGTTTG CAAAGGGAGA AAGTAGGAGA 961 TCTCTCTTGC GAGATGATCC CGCATTTTCT TGAAAGCTTT GCAGAGGCTA GCAGAATTAC 1021 CCTCCACGTT GATTGTCTGC GAGGCAAGAA TGATCATCAC CGTAGTGAGA GTGCGTTCAA 1081 GGCTCTTGCG GTTGCCATAA GAGAAGCCAC CTCGCCCAAT GGTACCAACG ATGTTCCCTC 1141 CACCAAAGGT GTTCTTATGT AGTGACACCG ATTATTTAAA GCTGCAGCAT ACGATATATA 1201 TACATGTGTA TATATGTATA CCTATGAATG TCAGTAAGTA TGTATACGAA CAGTATGATA 1261 CTGAAGATGA CAAGGTAATG CATCATTCTA TACGTGTCAT TCTGAACGAG GCGCGCTTTC 1321 CTTTTTTCTT TTTGCTTTTT CTTTTTTTTT CTCTTGAACT CGACGGATCA TATGCGGTGT 1381 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA 1441 TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG 1501 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC 1561 CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA 1621 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT 1681 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC 1741 GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA 1801 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG 1861 CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC 1921 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC 1981 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG 2041 AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGA TCGTGATTCT GGGTAGAAGA 2101 TCGGTCTGCA TTGGATGGTG GTAACGCATT TTTTTACACA CATTACTTGC CTCGAGCATC 2161 AAATGGTGGT TATTCGTGGA TCTATATCAC GTGATTTGCT TAAGAATTGT CGTTCATGGT 2221 GACACTTTTA GCTTTGACAT GATTAAGCTC ATCTCAATTG ATGTTATCTA AAGTCATTTC 2281 AACTATCTAA GATGTGGTTG TGATTGGGCC ATTTTGTGAA AGCCAGTACG CCAGCGTCAA 2341 TACACTCCCG TCAATTAGTT GCACCATGTC CACAAAATCA TATACCAGTA GAGCTGAGAC 2401 TCATGCAAGT CCGGTTGCAT CGAAATTGCT GAGACTGATG GATGAAAAGA AGACCAATTT 2461 GTGTGCTTCT CTTGACGTTC GTTCGACTGA TGAGCTATTG AAACTTGTTG AAACGTTGGG 2521 TCCATACATT TGCCTTTTGA AAACACACGT TGATATCTTG GATGATTTCA GTTATGAGGG 2581 TACTGTCGTT CCATTGAAAG CATTGGCAGA GAAATACAAG TTCTTGATAT TTGAGGACAG 2641 AAAATTCGCC GATATCGGTA ACACAGTCAA ATTACAATAT ACATCGGGCG TTTACCGTAT 2701 CGCAGAATGG TCTGATATCA CCAACGCCCA CGGGGTTACT GGTGCTGGTA TTGTTGCTGG 2761 CTTGAAACAA GGTGCGCAAG AGGTCACCAA AGAACCAAGG GGATTATTGA TGCTTGCTGA 2821 ATTGTCTTCC AAGGGTTCTC TAGCACACGG TGAATATACT AAGGGTACCG TTGATATTGC 2881 AAAGAGTGAT AAAGATTTCG TTATTGGGTT CATTGCTCAG AACGATATGG GAGGAAGAGA 2941 AGAAGGGTTT GATTGGCTAA TCATGACCCC AGGTGTAGGT TTAGACGACA AAGGCGATGC 3001 ATTGGGTCAG CAGTACAGAA CCGTCGACGA AGTTGTAAGT GGTGGATCAG ATATCATCAT 3061 TGTTGGCAGA GGACTTTTCG CCAAGGGTAG AGATCCTAAG GTTGAAGGTG AAAGATACAG 3121 AAATGCTGGA TGGGAAGCGT ACCAAAAGAG AATCAGCGCT CCCCATTAAT TATACAGGAA 3181 ACTTAATAGA ACAAATCACA TATTTAATCT AATAGCCACC TGCATTGGCA CGGTGCAACA 3241 CTACTTCAAC TTCATCTTAC AAAAAGATCA CGTGATCTGT TGTATTGAAC TGAAAATTTT 3301 TTGTTTGCTT CTCTCTCTCT CTCTTTCATT ATGTGAGATT TAAAAACCAG AAACTACATC 3361 ATCGATGTGA TTCTGGGTAG AAGATCGGTC TGCATTGGAT GGTGGTAACG CATTTTTTTA 3421 CACACATTAC TTGCCTCGAG CATCAAATGG TGGTTATTCG TGGATCTATA TCACGTGATT 3481 TGCTTAAGAA TTGTCGTTCA TGGTGACACG GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG 3541 TGAGGGTTAA TTCCGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 3601 TATCCGCTCA CAATTCCACA CAACATAGGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 3661 GCCTAATGAG TGAGGTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 3721 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 3781 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 3841 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 3901 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 3961 GCGTTGCTGG CGTTTTTCCA TAGGCTCGGC CCCCCTGACG AGCATCACAA AAATCGACGC 4021 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT CCCCCCTGGA 4081 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 4141 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG 4201 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 4261 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 4321 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 4381 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 4441 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 4501 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 4561 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 4621 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 4681 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 4741 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 4801 TGACTGCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 4861 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 4921 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 4981 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 5041 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 5101 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGAAAAAA AGCGGTTAGC 5161 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 5221 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 5281 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 5341 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 5401 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 5461 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 5521 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 5581 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 5641 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 5701 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TTATTATCAT GACATTAACC TATAAAAATA 5761 GGCGTATCAC GAGGCCCTTT CGTC //