LOCUS       PWJ1077.GB   5784 BP DS-DNA   CIRCULAR  SYN       14-SEP-1995
DEFINITION  Yeast integrative vector pWJ1077 with Kluveromyces lactis URA3
            marker and Saccharomyces cerevisiae HIS3 marker, complete sequence.
ACCESSION   Not submitted as of 4/2002
KEYWORDS    K. lactis URA3 adaptamer gene disruption
SOURCE      Cloning vector pRS303
  ORGANISM  Saccharomyces cerevisiae
REFERENCE   1
  AUTHORS   Reid RJ, Sunjevaric I, Kedacche M, Rothstein R.
  TITLE     Efficient PCR-based gene disruption in Saccharomyces strains using
            intergenic primers.
  JOURNAL   Yeast 2002 Mar 15;19(4):319-28
FEATURES             Location/Qualifiers
     CDS             4734..5594
                     /note="bla (amp-R)"
     CDS             503..1162
                     /note="HIS3"
     primer_bind     complement(2894..2918)
                     /note="Kli-3'"
     primer_bind     2471..2495
                     /note="Kli-5'"
     CDS             <2366..>3168
                     /note="K.lactis URA3"
     primer_bind     1993..2016
                     /note="c-Kl"
     primer_bind     complement(3522..3545)
                     /note="d-Kl"
     primer_bind     2083..2102
                     /note="d2-Kl"
     primer_bind     complement(3488..3509)
                     /note="u2-Kl"
     frag            3361..3515
                     /note="Repeat"
     frag            2083..2225
                     /note="Repeat"
BASE COUNT     1539 A   1281 C   1414 G   1550 T      0 OTHER
ORIGIN      -
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATAATTC CGTTTTAAGA GCTTGGTGAG CGCTAGGAGT CACTGCCAGG TATCGTTTGA
      241 ACACGGCATT AGTCAGGGAA GTCATAACAC AGTCCTTTCC CGCAATTTTC TTTTTCTATT
      301 ACTCTTGGCC TCCTCTAGTA CACTCTATAT TTTTTTATGC CTCGGTAATG ATTTTCATTT
      361 TTTTTTTTCC ACCTAGCGGA TGACTCTTTT TTTTTCTTAG CGATTGGCAT TATCACATAA
      421 TGAATTATAC ATTATATAAA GTAATGTGAT TTCTTCGAAG AATATACTAA AAAATGAGCA
      481 GGCAAGATAA ACGAAGGCAA AGATGACAGA GCAGAAAGCC CTAGTAAAGC GTATTACAAA
      541 TGAAACCAAG ATTCAGATTG CGATCTCTTT AAAGGGTGGT CCCCTAGCGA TAGAGCACTC
      601 GATCTTCCCA GAAAAAGAGG CAGAAGCAGT AGCAGAACAG GCCACACAAT CGCAAGTGAT
      661 TAACGTCCAC ACAGGTATAG GGTTTCTGGA CCATATGATA CATGCTCTGG CCAAGCATTC
      721 CGGCTGGTCG CTAATCGTTG AGTGCATTGG TGACTTACAC ATAGACGACC ATCACACCAC
      781 TGAAGACTGC GGGATTGCTC TCGGTCAAGC TTTTAAAGAG GCCCTACTGG CGCGTGGAGT
      841 AAAAAGGTTT GGATCAGGAT TTGCGCCTTT GGATGAGGCA CTTTCCAGAG CGGTGGTAGA
      901 TCTTTCGAAC AGGCCGTACG CAGTTGTCGA ACTTGGTTTG CAAAGGGAGA AAGTAGGAGA
      961 TCTCTCTTGC GAGATGATCC CGCATTTTCT TGAAAGCTTT GCAGAGGCTA GCAGAATTAC
     1021 CCTCCACGTT GATTGTCTGC GAGGCAAGAA TGATCATCAC CGTAGTGAGA GTGCGTTCAA
     1081 GGCTCTTGCG GTTGCCATAA GAGAAGCCAC CTCGCCCAAT GGTACCAACG ATGTTCCCTC
     1141 CACCAAAGGT GTTCTTATGT AGTGACACCG ATTATTTAAA GCTGCAGCAT ACGATATATA
     1201 TACATGTGTA TATATGTATA CCTATGAATG TCAGTAAGTA TGTATACGAA CAGTATGATA
     1261 CTGAAGATGA CAAGGTAATG CATCATTCTA TACGTGTCAT TCTGAACGAG GCGCGCTTTC
     1321 CTTTTTTCTT TTTGCTTTTT CTTTTTTTTT CTCTTGAACT CGACGGATCA TATGCGGTGT
     1381 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA
     1441 TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG
     1501 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC
     1561 CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA
     1621 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT
     1681 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC
     1741 GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA
     1801 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG
     1861 CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
     1921 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC
     1981 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG
     2041 AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGA TCGTGATTCT GGGTAGAAGA
     2101 TCGGTCTGCA TTGGATGGTG GTAACGCATT TTTTTACACA CATTACTTGC CTCGAGCATC
     2161 AAATGGTGGT TATTCGTGGA TCTATATCAC GTGATTTGCT TAAGAATTGT CGTTCATGGT
     2221 GACACTTTTA GCTTTGACAT GATTAAGCTC ATCTCAATTG ATGTTATCTA AAGTCATTTC
     2281 AACTATCTAA GATGTGGTTG TGATTGGGCC ATTTTGTGAA AGCCAGTACG CCAGCGTCAA
     2341 TACACTCCCG TCAATTAGTT GCACCATGTC CACAAAATCA TATACCAGTA GAGCTGAGAC
     2401 TCATGCAAGT CCGGTTGCAT CGAAATTGCT GAGACTGATG GATGAAAAGA AGACCAATTT
     2461 GTGTGCTTCT CTTGACGTTC GTTCGACTGA TGAGCTATTG AAACTTGTTG AAACGTTGGG
     2521 TCCATACATT TGCCTTTTGA AAACACACGT TGATATCTTG GATGATTTCA GTTATGAGGG
     2581 TACTGTCGTT CCATTGAAAG CATTGGCAGA GAAATACAAG TTCTTGATAT TTGAGGACAG
     2641 AAAATTCGCC GATATCGGTA ACACAGTCAA ATTACAATAT ACATCGGGCG TTTACCGTAT
     2701 CGCAGAATGG TCTGATATCA CCAACGCCCA CGGGGTTACT GGTGCTGGTA TTGTTGCTGG
     2761 CTTGAAACAA GGTGCGCAAG AGGTCACCAA AGAACCAAGG GGATTATTGA TGCTTGCTGA
     2821 ATTGTCTTCC AAGGGTTCTC TAGCACACGG TGAATATACT AAGGGTACCG TTGATATTGC
     2881 AAAGAGTGAT AAAGATTTCG TTATTGGGTT CATTGCTCAG AACGATATGG GAGGAAGAGA
     2941 AGAAGGGTTT GATTGGCTAA TCATGACCCC AGGTGTAGGT TTAGACGACA AAGGCGATGC
     3001 ATTGGGTCAG CAGTACAGAA CCGTCGACGA AGTTGTAAGT GGTGGATCAG ATATCATCAT
     3061 TGTTGGCAGA GGACTTTTCG CCAAGGGTAG AGATCCTAAG GTTGAAGGTG AAAGATACAG
     3121 AAATGCTGGA TGGGAAGCGT ACCAAAAGAG AATCAGCGCT CCCCATTAAT TATACAGGAA
     3181 ACTTAATAGA ACAAATCACA TATTTAATCT AATAGCCACC TGCATTGGCA CGGTGCAACA
     3241 CTACTTCAAC TTCATCTTAC AAAAAGATCA CGTGATCTGT TGTATTGAAC TGAAAATTTT
     3301 TTGTTTGCTT CTCTCTCTCT CTCTTTCATT ATGTGAGATT TAAAAACCAG AAACTACATC
     3361 ATCGATGTGA TTCTGGGTAG AAGATCGGTC TGCATTGGAT GGTGGTAACG CATTTTTTTA
     3421 CACACATTAC TTGCCTCGAG CATCAAATGG TGGTTATTCG TGGATCTATA TCACGTGATT
     3481 TGCTTAAGAA TTGTCGTTCA TGGTGACACG GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG
     3541 TGAGGGTTAA TTCCGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
     3601 TATCCGCTCA CAATTCCACA CAACATAGGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
     3661 GCCTAATGAG TGAGGTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
     3721 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     3781 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     3841 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     3901 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     3961 GCGTTGCTGG CGTTTTTCCA TAGGCTCGGC CCCCCTGACG AGCATCACAA AAATCGACGC
     4021 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT CCCCCCTGGA
     4081 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     4141 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG
     4201 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     4261 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     4321 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     4381 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     4441 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     4501 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     4561 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     4621 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     4681 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     4741 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     4801 TGACTGCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     4861 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     4921 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     4981 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     5041 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     5101 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGAAAAAA AGCGGTTAGC
     5161 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     5221 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     5281 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     5341 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     5401 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     5461 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     5521 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     5581 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     5641 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     5701 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TTATTATCAT GACATTAACC TATAAAAATA
     5761 GGCGTATCAC GAGGCCCTTT CGTC
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